escherichia coli strains dh5α Thermo Fisher Search Results


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  • 99
    Thermo Fisher e coli strain dh5α
    E Coli Strain Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 712 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli strains e coli strain dh5α
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
    Escherichia Coli Strains E Coli Strain Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 389 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli host strain dh5α
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
    Escherichia Coli Host Strain Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 76/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent dh5α escherichia coli strain
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
    Competent Dh5α Escherichia Coli Strain, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli strain dh5α mcr
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
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    Thermo Fisher escherichia coli electromax dh5α strain
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
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    Thermo Fisher reca e coli strain dh5α
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
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    Thermo Fisher escherichia coli k 12 strain dh5α
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
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    Thermo Fisher reagents escherichia coli strain dh5α
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
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    Thermo Fisher escherichia coli strain dh5α cells
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
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    Thermo Fisher e coli strain dh5alpha
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
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    Coomassie blue-stained SDS-PAGE of culture supernatants of E . coli strains <t>DH5α,</t> DH5α/pK18, and DH5α/pZH4. M, molecular mass markers.
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    Thermo Fisher growth conditions escherichia coli strain dh5α
    Coomassie blue-stained SDS-PAGE of culture supernatants of E . coli strains <t>DH5α,</t> DH5α/pK18, and DH5α/pZH4. M, molecular mass markers.
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    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Cell Culture

    Effect of chlorophyllin on the early growth phase of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698. Liquid cultures of bacteria (initial cell number: 10 6 cfu/mL) were incubated in Erlenmeyer flasks in standard LB medium (red) and in presence of a chlorophyllin concentration of 22 mg/L (green). Cells grew either illuminated with 12 mW/cm 2 (bright plots, upper row) or in darkness (grey plots, lower row). Depicted are measured values (circles) and fitted curves (solid lines) with corresponding 95% confidence limits (red and green areas). Dashed lines describe growth of example cultures in LB medium + MeOH/KOH (solvent of chlorophyllin).

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Effect of chlorophyllin on the early growth phase of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698. Liquid cultures of bacteria (initial cell number: 10 6 cfu/mL) were incubated in Erlenmeyer flasks in standard LB medium (red) and in presence of a chlorophyllin concentration of 22 mg/L (green). Cells grew either illuminated with 12 mW/cm 2 (bright plots, upper row) or in darkness (grey plots, lower row). Depicted are measured values (circles) and fitted curves (solid lines) with corresponding 95% confidence limits (red and green areas). Dashed lines describe growth of example cultures in LB medium + MeOH/KOH (solvent of chlorophyllin).

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Incubation, Concentration Assay

    ( A ) Chemical structure of isolated chlorophyllin. ( B ) Alterations of chlorophyllin’s absorption spectrum in darkness and ( C ) exposed to light with 12 mW/cm 2 . ( D ) Reduction of chlorophyllin concentration exposed to different light intensities. ( E ) Required light exposure times to sterilize an E. coli DH5α suspension culture initial cell number: ~10 6 cfu/mL) with a light intensity of 12 mW/cm 2 using different chlorophyllin concentrations. ( F ) Required light exposure times to sterilize an E. coli DH5α suspension culture (initial cell number: ~10 6 cfu/mL) with a chlorophyllin concentration of 25 mg/L and different light intensities. For 12.5% light intensity no sterilization was observed within 300 min. Depicted are means ± standard deviation.

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: ( A ) Chemical structure of isolated chlorophyllin. ( B ) Alterations of chlorophyllin’s absorption spectrum in darkness and ( C ) exposed to light with 12 mW/cm 2 . ( D ) Reduction of chlorophyllin concentration exposed to different light intensities. ( E ) Required light exposure times to sterilize an E. coli DH5α suspension culture initial cell number: ~10 6 cfu/mL) with a light intensity of 12 mW/cm 2 using different chlorophyllin concentrations. ( F ) Required light exposure times to sterilize an E. coli DH5α suspension culture (initial cell number: ~10 6 cfu/mL) with a chlorophyllin concentration of 25 mg/L and different light intensities. For 12.5% light intensity no sterilization was observed within 300 min. Depicted are means ± standard deviation.

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Isolation, Concentration Assay, Standard Deviation

    Schematic presentation of evaluation of CFU ability after incubation to chlorophyllin. Differently dense liquid cultures of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698 were supplemented with different chlorophyllin concentrations between 0.1 and 25 mg/L. Cells grew in 96-well matrix plates either illuminated with 12 mW/cm 2 ( A – C ) or protected from light ( D – F ). Samples (2.5 µL) were drawn at different time points and transferred onto LB agar plates. After overnight incubation at 37 °C in the dark, colony growth was analyzed. Dot size quantifies colony growth. Original pictures of the agar plates can be found in Figure S1 .

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Schematic presentation of evaluation of CFU ability after incubation to chlorophyllin. Differently dense liquid cultures of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698 were supplemented with different chlorophyllin concentrations between 0.1 and 25 mg/L. Cells grew in 96-well matrix plates either illuminated with 12 mW/cm 2 ( A – C ) or protected from light ( D – F ). Samples (2.5 µL) were drawn at different time points and transferred onto LB agar plates. After overnight incubation at 37 °C in the dark, colony growth was analyzed. Dot size quantifies colony growth. Original pictures of the agar plates can be found in Figure S1 .

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Incubation

    Fluorescence microscopic images of ( A ) Escherichia coli DH5α and ( B ) Escherichia coli NR698 after exposure to chlorophyllin (red fluorescence). Assumed modes of action of chlorophyllin against Gram-positive and Gram-negative bacteria. ( C ) Chlorophyllin (Chl) molecules cannot pass the intact outer membrane of Gram-negative bacteria. ( D ) Chlorophyllin is degraded in light. Degradation products can enter both Gram-negative and Gram-positive cells. The red crosses indicate cell death.

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Fluorescence microscopic images of ( A ) Escherichia coli DH5α and ( B ) Escherichia coli NR698 after exposure to chlorophyllin (red fluorescence). Assumed modes of action of chlorophyllin against Gram-positive and Gram-negative bacteria. ( C ) Chlorophyllin (Chl) molecules cannot pass the intact outer membrane of Gram-negative bacteria. ( D ) Chlorophyllin is degraded in light. Degradation products can enter both Gram-negative and Gram-positive cells. The red crosses indicate cell death.

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Fluorescence

    Coomassie blue-stained SDS-PAGE of culture supernatants of E . coli strains DH5α, DH5α/pK18, and DH5α/pZH4. M, molecular mass markers.

    Journal: Infection and Immunity

    Article Title: Identification and Characterization of a Novel Genomic Island Integrated at selC in Locus of Enterocyte Effacement-Negative, Shiga Toxin-Producing Escherichia coli

    doi: 10.1128/IAI.69.11.6863-6873.2001

    Figure Lengend Snippet: Coomassie blue-stained SDS-PAGE of culture supernatants of E . coli strains DH5α, DH5α/pK18, and DH5α/pZH4. M, molecular mass markers.

    Article Snippet: E. coli O157:H7 strain EDL933 and E. coli laboratory strain DH5α (Gibco-BRL, Eggenstein, Germany) were used as controls for cytotoxicity tests and PCR analyses.

    Techniques: Staining, SDS Page