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  • 99
    Thermo Fisher escherichia coli max efficiency dh 5α competent cells
    Escherichia Coli Max Efficiency Dh 5α Competent Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore escherichia coli dh5α cells
    Escherichia Coli Dh5α Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli dh5 α cells
    Immunoprecipitation to determine the specificity of DivIVA interactions. (A) Immunoprecipitation of GFP-DivIVA-cI-Spo0J complex (lane 1), GFP-Spo0J-cI-DivIVA complex (lane 2), GFP-FtsZ-cI-DivIVA complex (lane 3), and GFP-DivIVA-cI-FtsZ complex (lane 4). (B) Immunoprecipitation of cI-DivIVA-GFP-PrfA complex (lane 1). Protein extracts were immunoprecipitated with anti-GFP antibodies and were detected with anti-cI rabbit polyclonal antibodies by Western blotting as described in Materials and Methods. Lane M contained molecular weight markers. In panel B lane 2 contained crude extract of E. coli strain <t>DH5α</t> expressing cI-DivIVA, detected with anti-cI antibodies.
    Competent E Coli Dh5 α Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli e coli dh5α
    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli <t>DH5α,</t> ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p
    Escherichia Coli E Coli Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher e coli dh5α cells
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    E Coli Dh5α Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa escherichia coli dh5α cells
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Escherichia Coli Dh5α Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toyobo escherichia coli dh5α cells
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Escherichia Coli Dh5α Cells, supplied by Toyobo, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co escherichia coli dh5α cells
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Escherichia Coli Dh5α Cells, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioMed Diagnostics Inc escherichia coli dh5α cells
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Escherichia Coli Dh5α Cells, supplied by BioMed Diagnostics Inc, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene escherichia coli dh5α cells
    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli <t>DH5α.</t> B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.
    Escherichia Coli Dh5α Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli dh5α
    Effect of l -methionine- S -sulfoximine (MS) on the growth of various bacteria in broth culture. The bacterial strains indicated were inoculated into an appropriate broth at an initial cell density of 10 5 cells/ml, except M . avium (5 × 10 5 cells/ml) and L. pneumophila and E . coli (10 6 cells/ ml). Mycobacteria were cultured in supplemented 7H9 medium, L. pneumophila in yeast extract medium, and E . coli in Luria-Bertani medium. All bacterial cultures were grown in the presence of various concentrations of l -methionine- S -sulfoximine, as indicated, at 37°C and, except for E . coli , in a 5% CO 2 atmosphere. M . tuberculosis , M . bovis , and M . avium were grown for 4 wk; M . smegmatis and M . phlei for 3 d; L . pneumophila for 24 h; and E . coli for 6 h. For the enumeration of viable bacteria in each culture, aliquots were removed weekly ( M . tuberculosis , M . bovis , and M . avium ), daily ( M . smegmatis and M . phlei ), every 4 h ( L . pneumophila ), or hourly ( E . coli ), plated on solid medium, and incubated at 37°C in a 5% CO 2 atmosphere for 2 wk ( M . tuberculosis , M . bovis , and M . avium ), 3 d ( M . smegmatis and M . phlei ), 24 h ( L . pneumophila ), or 12 h ( E . coli ). All cultures were in duplicate. For each time point, the variation in the colony count averaged ∼5% and never exceeded 10%. (A) M. tuberculosis Erdman; (B) M. tuberculosis H37Rv; (C) M. tuberculosis H37Ra; (D) M. bovis strain 19210; (E) M. bovis strain BCG; (F) M. avium strain 25291; (G) M. smegmatis strain 1-2c; (H) M. phlei strain 11758; (I) L. pneumophila Philadelphia 1; (J) E. coli <t>DH5α.</t>
    E Coli Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 280 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega e coli dh5α cells
    Effect of l -methionine- S -sulfoximine (MS) on the growth of various bacteria in broth culture. The bacterial strains indicated were inoculated into an appropriate broth at an initial cell density of 10 5 cells/ml, except M . avium (5 × 10 5 cells/ml) and L. pneumophila and E . coli (10 6 cells/ ml). Mycobacteria were cultured in supplemented 7H9 medium, L. pneumophila in yeast extract medium, and E . coli in Luria-Bertani medium. All bacterial cultures were grown in the presence of various concentrations of l -methionine- S -sulfoximine, as indicated, at 37°C and, except for E . coli , in a 5% CO 2 atmosphere. M . tuberculosis , M . bovis , and M . avium were grown for 4 wk; M . smegmatis and M . phlei for 3 d; L . pneumophila for 24 h; and E . coli for 6 h. For the enumeration of viable bacteria in each culture, aliquots were removed weekly ( M . tuberculosis , M . bovis , and M . avium ), daily ( M . smegmatis and M . phlei ), every 4 h ( L . pneumophila ), or hourly ( E . coli ), plated on solid medium, and incubated at 37°C in a 5% CO 2 atmosphere for 2 wk ( M . tuberculosis , M . bovis , and M . avium ), 3 d ( M . smegmatis and M . phlei ), 24 h ( L . pneumophila ), or 12 h ( E . coli ). All cultures were in duplicate. For each time point, the variation in the colony count averaged ∼5% and never exceeded 10%. (A) M. tuberculosis Erdman; (B) M. tuberculosis H37Rv; (C) M. tuberculosis H37Ra; (D) M. bovis strain 19210; (E) M. bovis strain BCG; (F) M. avium strain 25291; (G) M. smegmatis strain 1-2c; (H) M. phlei strain 11758; (I) L. pneumophila Philadelphia 1; (J) E. coli <t>DH5α.</t>
    E Coli Dh5α Cells, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Agilent technologies escherichia coli dh5α cells
    Effect of l -methionine- S -sulfoximine (MS) on the growth of various bacteria in broth culture. The bacterial strains indicated were inoculated into an appropriate broth at an initial cell density of 10 5 cells/ml, except M . avium (5 × 10 5 cells/ml) and L. pneumophila and E . coli (10 6 cells/ ml). Mycobacteria were cultured in supplemented 7H9 medium, L. pneumophila in yeast extract medium, and E . coli in Luria-Bertani medium. All bacterial cultures were grown in the presence of various concentrations of l -methionine- S -sulfoximine, as indicated, at 37°C and, except for E . coli , in a 5% CO 2 atmosphere. M . tuberculosis , M . bovis , and M . avium were grown for 4 wk; M . smegmatis and M . phlei for 3 d; L . pneumophila for 24 h; and E . coli for 6 h. For the enumeration of viable bacteria in each culture, aliquots were removed weekly ( M . tuberculosis , M . bovis , and M . avium ), daily ( M . smegmatis and M . phlei ), every 4 h ( L . pneumophila ), or hourly ( E . coli ), plated on solid medium, and incubated at 37°C in a 5% CO 2 atmosphere for 2 wk ( M . tuberculosis , M . bovis , and M . avium ), 3 d ( M . smegmatis and M . phlei ), 24 h ( L . pneumophila ), or 12 h ( E . coli ). All cultures were in duplicate. For each time point, the variation in the colony count averaged ∼5% and never exceeded 10%. (A) M. tuberculosis Erdman; (B) M. tuberculosis H37Rv; (C) M. tuberculosis H37Ra; (D) M. bovis strain 19210; (E) M. bovis strain BCG; (F) M. avium strain 25291; (G) M. smegmatis strain 1-2c; (H) M. phlei strain 11758; (I) L. pneumophila Philadelphia 1; (J) E. coli <t>DH5α.</t>
    Escherichia Coli Dh5α Cells, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co e coli dh5α cells
    Effect of l -methionine- S -sulfoximine (MS) on the growth of various bacteria in broth culture. The bacterial strains indicated were inoculated into an appropriate broth at an initial cell density of 10 5 cells/ml, except M . avium (5 × 10 5 cells/ml) and L. pneumophila and E . coli (10 6 cells/ ml). Mycobacteria were cultured in supplemented 7H9 medium, L. pneumophila in yeast extract medium, and E . coli in Luria-Bertani medium. All bacterial cultures were grown in the presence of various concentrations of l -methionine- S -sulfoximine, as indicated, at 37°C and, except for E . coli , in a 5% CO 2 atmosphere. M . tuberculosis , M . bovis , and M . avium were grown for 4 wk; M . smegmatis and M . phlei for 3 d; L . pneumophila for 24 h; and E . coli for 6 h. For the enumeration of viable bacteria in each culture, aliquots were removed weekly ( M . tuberculosis , M . bovis , and M . avium ), daily ( M . smegmatis and M . phlei ), every 4 h ( L . pneumophila ), or hourly ( E . coli ), plated on solid medium, and incubated at 37°C in a 5% CO 2 atmosphere for 2 wk ( M . tuberculosis , M . bovis , and M . avium ), 3 d ( M . smegmatis and M . phlei ), 24 h ( L . pneumophila ), or 12 h ( E . coli ). All cultures were in duplicate. For each time point, the variation in the colony count averaged ∼5% and never exceeded 10%. (A) M. tuberculosis Erdman; (B) M. tuberculosis H37Rv; (C) M. tuberculosis H37Ra; (D) M. bovis strain 19210; (E) M. bovis strain BCG; (F) M. avium strain 25291; (G) M. smegmatis strain 1-2c; (H) M. phlei strain 11758; (I) L. pneumophila Philadelphia 1; (J) E. coli <t>DH5α.</t>
    E Coli Dh5α Cells, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 94/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa e coli dh5α competent cells
    Phenotypic detection of carbapenemases and extended-spectrum β-lactamase (ESBL) in E. coli BJ01 . a. Antibacterial susceptibility testing for BJ01 on the Mueller-Hinton agar (MHA). b. Antimicrobial susceptibility testing for BJ01 on MHA impregnated with 2 mL of ethylenediaminetetraacetic acid (EDTA) 5 mM. c. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 ESBLs ). d. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 NDM-1 ). EC600 NDM-1 was resistant to imipenem, meropenem, and cephalosporins except for ATM and showed no synergy between AMC and cephalosporins. e. Antimicrobial susceptibility testing for the <t>DH5α</t> transformant ( DH5α NDM-1 ). The phenotype was the same as that of EC600 NDM-1 . IMP, imipenem; MEM, meropenem; CRO, ceftriaxone; AMC,amoxicillin-clavulanic acid; CAZ, ceftazidime; ATM, aztreonam; FEP, cefepime.
    E Coli Dh5α Competent Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore e coli dh5α competent cells
    Phenotypic detection of carbapenemases and extended-spectrum β-lactamase (ESBL) in E. coli BJ01 . a. Antibacterial susceptibility testing for BJ01 on the Mueller-Hinton agar (MHA). b. Antimicrobial susceptibility testing for BJ01 on MHA impregnated with 2 mL of ethylenediaminetetraacetic acid (EDTA) 5 mM. c. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 ESBLs ). d. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 NDM-1 ). EC600 NDM-1 was resistant to imipenem, meropenem, and cephalosporins except for ATM and showed no synergy between AMC and cephalosporins. e. Antimicrobial susceptibility testing for the <t>DH5α</t> transformant ( DH5α NDM-1 ). The phenotype was the same as that of EC600 NDM-1 . IMP, imipenem; MEM, meropenem; CRO, ceftriaxone; AMC,amoxicillin-clavulanic acid; CAZ, ceftazidime; ATM, aztreonam; FEP, cefepime.
    E Coli Dh5α Competent Cells, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toyobo e coli dh5α competent cells
    Phenotypic detection of carbapenemases and extended-spectrum β-lactamase (ESBL) in E. coli BJ01 . a. Antibacterial susceptibility testing for BJ01 on the Mueller-Hinton agar (MHA). b. Antimicrobial susceptibility testing for BJ01 on MHA impregnated with 2 mL of ethylenediaminetetraacetic acid (EDTA) 5 mM. c. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 ESBLs ). d. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 NDM-1 ). EC600 NDM-1 was resistant to imipenem, meropenem, and cephalosporins except for ATM and showed no synergy between AMC and cephalosporins. e. Antimicrobial susceptibility testing for the <t>DH5α</t> transformant ( DH5α NDM-1 ). The phenotype was the same as that of EC600 NDM-1 . IMP, imipenem; MEM, meropenem; CRO, ceftriaxone; AMC,amoxicillin-clavulanic acid; CAZ, ceftazidime; ATM, aztreonam; FEP, cefepime.
    E Coli Dh5α Competent Cells, supplied by Toyobo, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    TaKaRa e coli dh5α electro cells
    Phenotypic detection of carbapenemases and extended-spectrum β-lactamase (ESBL) in E. coli BJ01 . a. Antibacterial susceptibility testing for BJ01 on the Mueller-Hinton agar (MHA). b. Antimicrobial susceptibility testing for BJ01 on MHA impregnated with 2 mL of ethylenediaminetetraacetic acid (EDTA) 5 mM. c. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 ESBLs ). d. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 NDM-1 ). EC600 NDM-1 was resistant to imipenem, meropenem, and cephalosporins except for ATM and showed no synergy between AMC and cephalosporins. e. Antimicrobial susceptibility testing for the <t>DH5α</t> transformant ( DH5α NDM-1 ). The phenotype was the same as that of EC600 NDM-1 . IMP, imipenem; MEM, meropenem; CRO, ceftriaxone; AMC,amoxicillin-clavulanic acid; CAZ, ceftazidime; ATM, aztreonam; FEP, cefepime.
    E Coli Dh5α Electro Cells, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Immunoprecipitation to determine the specificity of DivIVA interactions. (A) Immunoprecipitation of GFP-DivIVA-cI-Spo0J complex (lane 1), GFP-Spo0J-cI-DivIVA complex (lane 2), GFP-FtsZ-cI-DivIVA complex (lane 3), and GFP-DivIVA-cI-FtsZ complex (lane 4). (B) Immunoprecipitation of cI-DivIVA-GFP-PrfA complex (lane 1). Protein extracts were immunoprecipitated with anti-GFP antibodies and were detected with anti-cI rabbit polyclonal antibodies by Western blotting as described in Materials and Methods. Lane M contained molecular weight markers. In panel B lane 2 contained crude extract of E. coli strain DH5α expressing cI-DivIVA, detected with anti-cI antibodies.

    Journal: Journal of Bacteriology

    Article Title: Streptococcus pneumoniae DivIVA: Localization and Interactions in a MinCD-Free Context ▿ DivIVA: Localization and Interactions in a MinCD-Free Context ▿ †

    doi: 10.1128/JB.01168-06

    Figure Lengend Snippet: Immunoprecipitation to determine the specificity of DivIVA interactions. (A) Immunoprecipitation of GFP-DivIVA-cI-Spo0J complex (lane 1), GFP-Spo0J-cI-DivIVA complex (lane 2), GFP-FtsZ-cI-DivIVA complex (lane 3), and GFP-DivIVA-cI-FtsZ complex (lane 4). (B) Immunoprecipitation of cI-DivIVA-GFP-PrfA complex (lane 1). Protein extracts were immunoprecipitated with anti-GFP antibodies and were detected with anti-cI rabbit polyclonal antibodies by Western blotting as described in Materials and Methods. Lane M contained molecular weight markers. In panel B lane 2 contained crude extract of E. coli strain DH5α expressing cI-DivIVA, detected with anti-cI antibodies.

    Article Snippet: The ligation mixture was transformed into E. coli DH5α competent cells (Invitrogen), and transformants were selected after overnight growth on LB medium plates with ampicillin.

    Techniques: Immunoprecipitation, Western Blot, Molecular Weight, Expressing

    Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Influence of different chlorophyllin concentrations on the growth of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168 and ( C ) Escherichia coli NR698 (initial cell number: ~10 8 cfu/mL). Bacteria were cultured for 24 h at 37 °C exposed to light (12 mW/cm 2 ; bright plots, upper row) or in darkness (grey plots, lower row). To determine the lower limit of efficacy, additional chlorophyllin concentrations were tested: ( D ) Escherichia coli DH5α, 20↑100 mg/L ( E ) Bacillus subtilis 168, 1.0↓0.01 mg/L and ( F ) Escherichia coli NR698, 1.0↓0.01 mg/L. Depicted are means (growth curves) together with corresponding 95% confidence limits (bar charts). Blue numbers indicate respective chlorophyllin concentrations. * p

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Cell Culture

    Effect of chlorophyllin on the early growth phase of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698. Liquid cultures of bacteria (initial cell number: 10 6 cfu/mL) were incubated in Erlenmeyer flasks in standard LB medium (red) and in presence of a chlorophyllin concentration of 22 mg/L (green). Cells grew either illuminated with 12 mW/cm 2 (bright plots, upper row) or in darkness (grey plots, lower row). Depicted are measured values (circles) and fitted curves (solid lines) with corresponding 95% confidence limits (red and green areas). Dashed lines describe growth of example cultures in LB medium + MeOH/KOH (solvent of chlorophyllin).

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Effect of chlorophyllin on the early growth phase of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698. Liquid cultures of bacteria (initial cell number: 10 6 cfu/mL) were incubated in Erlenmeyer flasks in standard LB medium (red) and in presence of a chlorophyllin concentration of 22 mg/L (green). Cells grew either illuminated with 12 mW/cm 2 (bright plots, upper row) or in darkness (grey plots, lower row). Depicted are measured values (circles) and fitted curves (solid lines) with corresponding 95% confidence limits (red and green areas). Dashed lines describe growth of example cultures in LB medium + MeOH/KOH (solvent of chlorophyllin).

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Incubation, Concentration Assay

    ( A ) Chemical structure of isolated chlorophyllin. ( B ) Alterations of chlorophyllin’s absorption spectrum in darkness and ( C ) exposed to light with 12 mW/cm 2 . ( D ) Reduction of chlorophyllin concentration exposed to different light intensities. ( E ) Required light exposure times to sterilize an E. coli DH5α suspension culture initial cell number: ~10 6 cfu/mL) with a light intensity of 12 mW/cm 2 using different chlorophyllin concentrations. ( F ) Required light exposure times to sterilize an E. coli DH5α suspension culture (initial cell number: ~10 6 cfu/mL) with a chlorophyllin concentration of 25 mg/L and different light intensities. For 12.5% light intensity no sterilization was observed within 300 min. Depicted are means ± standard deviation.

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: ( A ) Chemical structure of isolated chlorophyllin. ( B ) Alterations of chlorophyllin’s absorption spectrum in darkness and ( C ) exposed to light with 12 mW/cm 2 . ( D ) Reduction of chlorophyllin concentration exposed to different light intensities. ( E ) Required light exposure times to sterilize an E. coli DH5α suspension culture initial cell number: ~10 6 cfu/mL) with a light intensity of 12 mW/cm 2 using different chlorophyllin concentrations. ( F ) Required light exposure times to sterilize an E. coli DH5α suspension culture (initial cell number: ~10 6 cfu/mL) with a chlorophyllin concentration of 25 mg/L and different light intensities. For 12.5% light intensity no sterilization was observed within 300 min. Depicted are means ± standard deviation.

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Isolation, Concentration Assay, Standard Deviation

    Schematic presentation of evaluation of CFU ability after incubation to chlorophyllin. Differently dense liquid cultures of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698 were supplemented with different chlorophyllin concentrations between 0.1 and 25 mg/L. Cells grew in 96-well matrix plates either illuminated with 12 mW/cm 2 ( A – C ) or protected from light ( D – F ). Samples (2.5 µL) were drawn at different time points and transferred onto LB agar plates. After overnight incubation at 37 °C in the dark, colony growth was analyzed. Dot size quantifies colony growth. Original pictures of the agar plates can be found in Figure S1 .

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Schematic presentation of evaluation of CFU ability after incubation to chlorophyllin. Differently dense liquid cultures of ( A ) Escherichia coli DH5α, ( B ) Bacillus subtilis 168, and ( C ) Escherichia coli NR698 were supplemented with different chlorophyllin concentrations between 0.1 and 25 mg/L. Cells grew in 96-well matrix plates either illuminated with 12 mW/cm 2 ( A – C ) or protected from light ( D – F ). Samples (2.5 µL) were drawn at different time points and transferred onto LB agar plates. After overnight incubation at 37 °C in the dark, colony growth was analyzed. Dot size quantifies colony growth. Original pictures of the agar plates can be found in Figure S1 .

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Incubation

    Fluorescence microscopic images of ( A ) Escherichia coli DH5α and ( B ) Escherichia coli NR698 after exposure to chlorophyllin (red fluorescence). Assumed modes of action of chlorophyllin against Gram-positive and Gram-negative bacteria. ( C ) Chlorophyllin (Chl) molecules cannot pass the intact outer membrane of Gram-negative bacteria. ( D ) Chlorophyllin is degraded in light. Degradation products can enter both Gram-negative and Gram-positive cells. The red crosses indicate cell death.

    Journal: Microorganisms

    Article Title: What an Escherichia coli Mutant Can Teach Us About the Antibacterial Effect of Chlorophyllin

    doi: 10.3390/microorganisms7020059

    Figure Lengend Snippet: Fluorescence microscopic images of ( A ) Escherichia coli DH5α and ( B ) Escherichia coli NR698 after exposure to chlorophyllin (red fluorescence). Assumed modes of action of chlorophyllin against Gram-positive and Gram-negative bacteria. ( C ) Chlorophyllin (Chl) molecules cannot pass the intact outer membrane of Gram-negative bacteria. ( D ) Chlorophyllin is degraded in light. Degradation products can enter both Gram-negative and Gram-positive cells. The red crosses indicate cell death.

    Article Snippet: Bacteria Strains and Cell Culture Experiments were performed with E. coli DH5α (Invitrogen, Carlsbad, CA, USA), E. coli NR698 (MC4100 lptD4213 ; kindly provided by M. Grabowicz, Princeton University, NJ, USA) [ ] and B. subtilis 168 (trpC2 ; laboratory stock).

    Techniques: Fluorescence

    Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.

    Journal: Microbial Cell Factories

    Article Title: An engineered autotransporter-based surface expression vector enables efficient display of Affibody molecules on OmpT-negative E. coli as well as protease-mediated secretion in OmpT-positive strains

    doi: 10.1186/s12934-014-0179-z

    Figure Lengend Snippet: Evaluation of OmpT-mediated release and small-scale purification of displayed Affibody molecule. A . Flow-cytometric analysis for comparison of surface expression of recombinant proteins between p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. B . SDS-PAGE showing IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-negative E. coli BL21 and p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Supernatants from non-induced samples, indicated with (−), were included as controls. C . Mass spectrum from ESI-MS analysis of IMAC-purified supernatants from p AraBAD -Z IgG -EC in OmpT-positive E. coli DH5α. Theoretical molecular weight of the OmpT-cleaved Z IgG -His 6 is 9712 Da. D . SPR-based biosensor analysis on the IMAC-purified Z IgG -His 6 . Response units (RU) on the y-axis and time on the x-axis. Representative sensorgrams from injection of Z IgG -His 6 at three different concentrations over human IgG immobilized on the chip surface. Injections were performed in duplicates.

    Article Snippet: The plasmid and PCR fragment were ligated using a T4 DNA ligase (New England Biolabs), and transformed to E. coli DH5α cells (Invitrogen) by heat shock.

    Techniques: Purification, Flow Cytometry, Expressing, Recombinant, SDS Page, Mass Spectrometry, Molecular Weight, SPR Assay, Injection, Chromatin Immunoprecipitation

    Effect of l -methionine- S -sulfoximine (MS) on the growth of various bacteria in broth culture. The bacterial strains indicated were inoculated into an appropriate broth at an initial cell density of 10 5 cells/ml, except M . avium (5 × 10 5 cells/ml) and L. pneumophila and E . coli (10 6 cells/ ml). Mycobacteria were cultured in supplemented 7H9 medium, L. pneumophila in yeast extract medium, and E . coli in Luria-Bertani medium. All bacterial cultures were grown in the presence of various concentrations of l -methionine- S -sulfoximine, as indicated, at 37°C and, except for E . coli , in a 5% CO 2 atmosphere. M . tuberculosis , M . bovis , and M . avium were grown for 4 wk; M . smegmatis and M . phlei for 3 d; L . pneumophila for 24 h; and E . coli for 6 h. For the enumeration of viable bacteria in each culture, aliquots were removed weekly ( M . tuberculosis , M . bovis , and M . avium ), daily ( M . smegmatis and M . phlei ), every 4 h ( L . pneumophila ), or hourly ( E . coli ), plated on solid medium, and incubated at 37°C in a 5% CO 2 atmosphere for 2 wk ( M . tuberculosis , M . bovis , and M . avium ), 3 d ( M . smegmatis and M . phlei ), 24 h ( L . pneumophila ), or 12 h ( E . coli ). All cultures were in duplicate. For each time point, the variation in the colony count averaged ∼5% and never exceeded 10%. (A) M. tuberculosis Erdman; (B) M. tuberculosis H37Rv; (C) M. tuberculosis H37Ra; (D) M. bovis strain 19210; (E) M. bovis strain BCG; (F) M. avium strain 25291; (G) M. smegmatis strain 1-2c; (H) M. phlei strain 11758; (I) L. pneumophila Philadelphia 1; (J) E. coli DH5α.

    Journal: The Journal of Experimental Medicine

    Article Title: An Inhibitor of Exported Mycobacterium tuberculosis Glutamine Synthetase Selectively Blocks the Growth of Pathogenic Mycobacteria in Axenic Culture and in Human Monocytes: Extracellular Proteins as Potential Novel Drug Targets

    doi:

    Figure Lengend Snippet: Effect of l -methionine- S -sulfoximine (MS) on the growth of various bacteria in broth culture. The bacterial strains indicated were inoculated into an appropriate broth at an initial cell density of 10 5 cells/ml, except M . avium (5 × 10 5 cells/ml) and L. pneumophila and E . coli (10 6 cells/ ml). Mycobacteria were cultured in supplemented 7H9 medium, L. pneumophila in yeast extract medium, and E . coli in Luria-Bertani medium. All bacterial cultures were grown in the presence of various concentrations of l -methionine- S -sulfoximine, as indicated, at 37°C and, except for E . coli , in a 5% CO 2 atmosphere. M . tuberculosis , M . bovis , and M . avium were grown for 4 wk; M . smegmatis and M . phlei for 3 d; L . pneumophila for 24 h; and E . coli for 6 h. For the enumeration of viable bacteria in each culture, aliquots were removed weekly ( M . tuberculosis , M . bovis , and M . avium ), daily ( M . smegmatis and M . phlei ), every 4 h ( L . pneumophila ), or hourly ( E . coli ), plated on solid medium, and incubated at 37°C in a 5% CO 2 atmosphere for 2 wk ( M . tuberculosis , M . bovis , and M . avium ), 3 d ( M . smegmatis and M . phlei ), 24 h ( L . pneumophila ), or 12 h ( E . coli ). All cultures were in duplicate. For each time point, the variation in the colony count averaged ∼5% and never exceeded 10%. (A) M. tuberculosis Erdman; (B) M. tuberculosis H37Rv; (C) M. tuberculosis H37Ra; (D) M. bovis strain 19210; (E) M. bovis strain BCG; (F) M. avium strain 25291; (G) M. smegmatis strain 1-2c; (H) M. phlei strain 11758; (I) L. pneumophila Philadelphia 1; (J) E. coli DH5α.

    Article Snippet: E. coli DH5α, L. pneumophila Philadelphia 1, the M. tuberculosis strains Erdman (35801; American Type Culture Collection [ATCC]), H37Rv (25618; ATCC), and H37Ra (25177; ATCC), M. bovis (19210; ATCC), M . bovis BCG (bacille Calmette-Guérin [19274; ATCC]), M. phlei (11758; ATCC), M. smegmatis (14468; ATCC), and Mycobacterium avium (25291; ATCC) were cultured as described ( ).

    Techniques: Mass Spectrometry, Cell Culture, Incubation

    Phenotypic detection of carbapenemases and extended-spectrum β-lactamase (ESBL) in E. coli BJ01 . a. Antibacterial susceptibility testing for BJ01 on the Mueller-Hinton agar (MHA). b. Antimicrobial susceptibility testing for BJ01 on MHA impregnated with 2 mL of ethylenediaminetetraacetic acid (EDTA) 5 mM. c. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 ESBLs ). d. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 NDM-1 ). EC600 NDM-1 was resistant to imipenem, meropenem, and cephalosporins except for ATM and showed no synergy between AMC and cephalosporins. e. Antimicrobial susceptibility testing for the DH5α transformant ( DH5α NDM-1 ). The phenotype was the same as that of EC600 NDM-1 . IMP, imipenem; MEM, meropenem; CRO, ceftriaxone; AMC,amoxicillin-clavulanic acid; CAZ, ceftazidime; ATM, aztreonam; FEP, cefepime.

    Journal: PLoS ONE

    Article Title: Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

    doi: 10.1371/journal.pone.0066666

    Figure Lengend Snippet: Phenotypic detection of carbapenemases and extended-spectrum β-lactamase (ESBL) in E. coli BJ01 . a. Antibacterial susceptibility testing for BJ01 on the Mueller-Hinton agar (MHA). b. Antimicrobial susceptibility testing for BJ01 on MHA impregnated with 2 mL of ethylenediaminetetraacetic acid (EDTA) 5 mM. c. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 ESBLs ). d. Antimicrobial susceptibility testing for the EC600 transconjugant ( EC600 NDM-1 ). EC600 NDM-1 was resistant to imipenem, meropenem, and cephalosporins except for ATM and showed no synergy between AMC and cephalosporins. e. Antimicrobial susceptibility testing for the DH5α transformant ( DH5α NDM-1 ). The phenotype was the same as that of EC600 NDM-1 . IMP, imipenem; MEM, meropenem; CRO, ceftriaxone; AMC,amoxicillin-clavulanic acid; CAZ, ceftazidime; ATM, aztreonam; FEP, cefepime.

    Article Snippet: Plasmid DNA was extracted using a Qiagen Mini Kit (Qiagen, Hilden, Germany) and was transformed into E. coli DH5α -competent cells (TAKARA, Shanghai, China).

    Techniques:

    Polymerase chain reaction-amplified CTX-M-57, TEM-1, and NDM-1 genes in BJ01 (1), EC600 ESBL (2), EC600 NDM-1 (3), and DH5α NDM-1 (4). CTX-M-57 and TEM-1 were detected in BJ01 and EC600 ESBL ; bla NDM-1 was detected in BJ01, E600 NDM-1 , and DH5α NDM-1 .

    Journal: PLoS ONE

    Article Title: Identification and Characterization of the First Escherichia coli Strain Carrying NDM-1 Gene in China

    doi: 10.1371/journal.pone.0066666

    Figure Lengend Snippet: Polymerase chain reaction-amplified CTX-M-57, TEM-1, and NDM-1 genes in BJ01 (1), EC600 ESBL (2), EC600 NDM-1 (3), and DH5α NDM-1 (4). CTX-M-57 and TEM-1 were detected in BJ01 and EC600 ESBL ; bla NDM-1 was detected in BJ01, E600 NDM-1 , and DH5α NDM-1 .

    Article Snippet: Plasmid DNA was extracted using a Qiagen Mini Kit (Qiagen, Hilden, Germany) and was transformed into E. coli DH5α -competent cells (TAKARA, Shanghai, China).

    Techniques: Polymerase Chain Reaction, Amplification, Transmission Electron Microscopy