escherichia coli cultures Thermo Fisher Search Results


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  • 92
    ATCC escherichia coli
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    Millipore e coli lps
    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli <t>LPS,</t> poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed <t>Th1/Th2,</t> Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.
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    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli <t>LPS,</t> poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed <t>Th1/Th2,</t> Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.
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    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli <t>LPS,</t> poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed <t>Th1/Th2,</t> Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.
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    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli <t>LPS,</t> poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed <t>Th1/Th2,</t> Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.
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    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli <t>LPS,</t> poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed <t>Th1/Th2,</t> Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.
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    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli <t>LPS,</t> poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed <t>Th1/Th2,</t> Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.
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    Thermo Fisher culture conditions escherichia coli one shot top10 electrocomp
    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli <t>LPS,</t> poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed <t>Th1/Th2,</t> Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.
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    Image Search Results


    T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli LPS, poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed Th1/Th2, Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.

    Journal: BMC Immunology

    Article Title: Differential intracellular fate of Burkholderia pseudomallei 844 and Burkholderia thailandensis UE5 in human monocyte-derived dendritic cells and macrophages

    doi: 10.1186/1471-2172-10-20

    Figure Lengend Snippet: T helper cell differentiation profiles driven by Bp -844- or Bt -UE5-stimulated MoDCs . MoDCs were allowed to mature after priming with PFA-fixed Bp -844 or Bt -UE5 for 24 hr. These matured MoDCs were then co-cultured with allogeneic T cells for approximately 2 weeks as detailed in the Methods section. Afterward, the resting differentiated T cells were restimulated with PMA/ionomycin and their intracellular IL-4 and IFN-γ levels were analyzed by flow cytometry. E. coli LPS, poly I:C and E.coli LPS + PGE2 were used as referencing stimuli for mixed Th1/Th2, Th1 and Th2, respectively. Results (mean and SEM) of the T cell responses from 5 different MoDC donors interacting with a same batch of allogeneic T cells are shown as per cent of responsive positive T cells in A and a scatter plot from one representative donor is shown in B. Circles indicate the predominant Th1 cell population induced by Bp -844 and Bt -UE5.

    Article Snippet: Each set of experiments always included control agents referencing for different T cell polarization, i.e., 5 μg/ml E. coli LPS (Sigma-Aldrich) for mixed Th1/Th2, 100 μg/ml poly I:C (Invitrogen, Carlsbad, CA) for Th1 and 5 μg/ml E. coli LPS plus 10 μg/ml prostagrandin E2 (PGE2, Invitrogen) for Th2 [ ].

    Techniques: Cell Differentiation, Cell Culture, Flow Cytometry, Cytometry