escherichia coli cultures Thermo Fisher Search Results


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  • 99
    Thermo Fisher competent escherichia coli cells
    Competent Escherichia Coli Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1310 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cultures escherichia coli dh10b
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    Thermo Fisher escherichia coli
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    Thermo Fisher escherichia coli dh5α
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    Thermo Fisher escherichia coli invαf
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    Thermo Fisher magicmedia escherichia coli expression medium
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    Thermo Fisher culture medium e coli dh5α
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    Thermo Fisher phrodo escherichia coli bioparticles
    F4/80 and CD11b expression identifies macrophage subsets in neonatal and adult colon (a) F4/80 and CD11b expression by live CD45 + SiglecF − Ly6G − CD11c lo colonic LP cells from neonatal (day 2), day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (b) Absolute numbers of total F4/80 + CD11b + cells per colon in day 2, day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (c) CD64 and CX3CR1-GFP expression by F4/80 + CD11b + colonic LP cells from newborn (day 2; black line) and 8 week old (green line) CX3CR1 +/gfp mice. Shaded histogram represents isotype control. (d) Phagocytic activity of F4/80 + CD11b + colonic LP cells from newborn (day 2) or adult CX3CR1 +/gfp mice measured by the uptake of <t>pHrodo</t> <t>Escherichia</t> coli <t>bioparticles</t> for 30-60 mins at 37°C or 4°C as a control (shaded histograms). (e) Morphological appearance of F4/80 + CD11b + colonic mφ from newborn and adult mice. Scale bar 25μm. (f) qRT-PCR of mRNA for IL10 and TNFα by F4/80 hi LP mφ from newborn (day 1) or adult mice, and BM-derived mφ (BMM). Results are mean expression relative to cyclophilin A (CPA) + 1SD obtained using the 2 −dΔC(t) , with BMM set to 1. ** P
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    Thermo Fisher dh10bac e coli
    F4/80 and CD11b expression identifies macrophage subsets in neonatal and adult colon (a) F4/80 and CD11b expression by live CD45 + SiglecF − Ly6G − CD11c lo colonic LP cells from neonatal (day 2), day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (b) Absolute numbers of total F4/80 + CD11b + cells per colon in day 2, day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (c) CD64 and CX3CR1-GFP expression by F4/80 + CD11b + colonic LP cells from newborn (day 2; black line) and 8 week old (green line) CX3CR1 +/gfp mice. Shaded histogram represents isotype control. (d) Phagocytic activity of F4/80 + CD11b + colonic LP cells from newborn (day 2) or adult CX3CR1 +/gfp mice measured by the uptake of <t>pHrodo</t> <t>Escherichia</t> coli <t>bioparticles</t> for 30-60 mins at 37°C or 4°C as a control (shaded histograms). (e) Morphological appearance of F4/80 + CD11b + colonic mφ from newborn and adult mice. Scale bar 25μm. (f) qRT-PCR of mRNA for IL10 and TNFα by F4/80 hi LP mφ from newborn (day 1) or adult mice, and BM-derived mφ (BMM). Results are mean expression relative to cyclophilin A (CPA) + 1SD obtained using the 2 −dΔC(t) , with BMM set to 1. ** P
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    Thermo Fisher e coli bl21
    F4/80 and CD11b expression identifies macrophage subsets in neonatal and adult colon (a) F4/80 and CD11b expression by live CD45 + SiglecF − Ly6G − CD11c lo colonic LP cells from neonatal (day 2), day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (b) Absolute numbers of total F4/80 + CD11b + cells per colon in day 2, day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (c) CD64 and CX3CR1-GFP expression by F4/80 + CD11b + colonic LP cells from newborn (day 2; black line) and 8 week old (green line) CX3CR1 +/gfp mice. Shaded histogram represents isotype control. (d) Phagocytic activity of F4/80 + CD11b + colonic LP cells from newborn (day 2) or adult CX3CR1 +/gfp mice measured by the uptake of <t>pHrodo</t> <t>Escherichia</t> coli <t>bioparticles</t> for 30-60 mins at 37°C or 4°C as a control (shaded histograms). (e) Morphological appearance of F4/80 + CD11b + colonic mφ from newborn and adult mice. Scale bar 25μm. (f) qRT-PCR of mRNA for IL10 and TNFα by F4/80 hi LP mφ from newborn (day 1) or adult mice, and BM-derived mφ (BMM). Results are mean expression relative to cyclophilin A (CPA) + 1SD obtained using the 2 −dΔC(t) , with BMM set to 1. ** P
    E Coli Bl21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 3722 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher culture conditions e coli top10 cells
    F4/80 and CD11b expression identifies macrophage subsets in neonatal and adult colon (a) F4/80 and CD11b expression by live CD45 + SiglecF − Ly6G − CD11c lo colonic LP cells from neonatal (day 2), day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (b) Absolute numbers of total F4/80 + CD11b + cells per colon in day 2, day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (c) CD64 and CX3CR1-GFP expression by F4/80 + CD11b + colonic LP cells from newborn (day 2; black line) and 8 week old (green line) CX3CR1 +/gfp mice. Shaded histogram represents isotype control. (d) Phagocytic activity of F4/80 + CD11b + colonic LP cells from newborn (day 2) or adult CX3CR1 +/gfp mice measured by the uptake of <t>pHrodo</t> <t>Escherichia</t> coli <t>bioparticles</t> for 30-60 mins at 37°C or 4°C as a control (shaded histograms). (e) Morphological appearance of F4/80 + CD11b + colonic mφ from newborn and adult mice. Scale bar 25μm. (f) qRT-PCR of mRNA for IL10 and TNFα by F4/80 hi LP mφ from newborn (day 1) or adult mice, and BM-derived mφ (BMM). Results are mean expression relative to cyclophilin A (CPA) + 1SD obtained using the 2 −dΔC(t) , with BMM set to 1. ** P
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    Thermo Fisher e coli dna
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    E Coli Dna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 153 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher expression host escherichia coli bl21 star de3
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    Expression Host Escherichia Coli Bl21 Star De3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 81/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli strain top10
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    Escherichia Coli Strain Top10, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 515 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli dh5α cells
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    Escherichia Coli Dh5α Cells, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 520 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli strain dh5a
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
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    Thermo Fisher escherichia coli strain bl21
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
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    Thermo Fisher media escherichia coli xl10 gold
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    Media Escherichia Coli Xl10 Gold, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli bl21 star de3
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    Escherichia Coli Bl21 Star De3, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli strain dh10b
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    Escherichia Coli Strain Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain dh10b/product/Thermo Fisher
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    Thermo Fisher escherichia coli strain dh5α
    Promotion of plasma cell differentiation and Ig production in lupus B cells by <t>ALD-DNA.</t> Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
    Escherichia Coli Strain Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 857 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain dh5α/product/Thermo Fisher
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    Image Search Results


    F4/80 and CD11b expression identifies macrophage subsets in neonatal and adult colon (a) F4/80 and CD11b expression by live CD45 + SiglecF − Ly6G − CD11c lo colonic LP cells from neonatal (day 2), day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (b) Absolute numbers of total F4/80 + CD11b + cells per colon in day 2, day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (c) CD64 and CX3CR1-GFP expression by F4/80 + CD11b + colonic LP cells from newborn (day 2; black line) and 8 week old (green line) CX3CR1 +/gfp mice. Shaded histogram represents isotype control. (d) Phagocytic activity of F4/80 + CD11b + colonic LP cells from newborn (day 2) or adult CX3CR1 +/gfp mice measured by the uptake of pHrodo Escherichia coli bioparticles for 30-60 mins at 37°C or 4°C as a control (shaded histograms). (e) Morphological appearance of F4/80 + CD11b + colonic mφ from newborn and adult mice. Scale bar 25μm. (f) qRT-PCR of mRNA for IL10 and TNFα by F4/80 hi LP mφ from newborn (day 1) or adult mice, and BM-derived mφ (BMM). Results are mean expression relative to cyclophilin A (CPA) + 1SD obtained using the 2 −dΔC(t) , with BMM set to 1. ** P

    Journal: Nature immunology

    Article Title: Constant replenishment from circulating monocytes maintains the macrophage pool in adult intestine

    doi: 10.1038/ni.2967

    Figure Lengend Snippet: F4/80 and CD11b expression identifies macrophage subsets in neonatal and adult colon (a) F4/80 and CD11b expression by live CD45 + SiglecF − Ly6G − CD11c lo colonic LP cells from neonatal (day 2), day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (b) Absolute numbers of total F4/80 + CD11b + cells per colon in day 2, day 14, day 21, day 35 and 9 week old CX3CR1 +/gfp mice. (c) CD64 and CX3CR1-GFP expression by F4/80 + CD11b + colonic LP cells from newborn (day 2; black line) and 8 week old (green line) CX3CR1 +/gfp mice. Shaded histogram represents isotype control. (d) Phagocytic activity of F4/80 + CD11b + colonic LP cells from newborn (day 2) or adult CX3CR1 +/gfp mice measured by the uptake of pHrodo Escherichia coli bioparticles for 30-60 mins at 37°C or 4°C as a control (shaded histograms). (e) Morphological appearance of F4/80 + CD11b + colonic mφ from newborn and adult mice. Scale bar 25μm. (f) qRT-PCR of mRNA for IL10 and TNFα by F4/80 hi LP mφ from newborn (day 1) or adult mice, and BM-derived mφ (BMM). Results are mean expression relative to cyclophilin A (CPA) + 1SD obtained using the 2 −dΔC(t) , with BMM set to 1. ** P

    Article Snippet: Assessment of phagocytosis 1-3 × 106 cells were cultured with pHrodo Escherichia coli bioparticles (Life Technologies) according to the manufacturer’s guidelines and analyzed by flow cytometry.

    Techniques: Expressing, Mouse Assay, Activity Assay, Quantitative RT-PCR, Derivative Assay

    Promotion of plasma cell differentiation and Ig production in lupus B cells by ALD-DNA. Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P

    Journal: PLoS ONE

    Article Title: Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production

    doi: 10.1371/journal.pone.0109095

    Figure Lengend Snippet: Promotion of plasma cell differentiation and Ig production in lupus B cells by ALD-DNA. Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P

    Article Snippet: CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences).

    Techniques: Cell Differentiation, Mouse Assay, Cell Culture, Expressing, Flow Cytometry, Cytometry, Generated, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Up-regulation of CD86 and MHC class II expression in naïve B cells by ALD-DNA stimulation. ( A ) Naïve B cells were incubated with ALD-DNA (50 µg/ml) in the presence or absence of LPS ranging from 0.01 µg/mL to 10 µg/ml for 72 h. The numbers of CD86-expressing B cells after each treatment were determined by flow cytometry. ( B ) Naive B cells were stimulated with ALD-DNA or E. coli single-stranded (ss) DNA (both at 50 µg/ml) for 72 h. CD86 and MHC class II expression was determined by performing flow cytometry. Histogram plots show the mean fluorescence intensity (MFI) of these surface molecules. The data are representative of three independent experiments.

    Journal: PLoS ONE

    Article Title: Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production

    doi: 10.1371/journal.pone.0109095

    Figure Lengend Snippet: Up-regulation of CD86 and MHC class II expression in naïve B cells by ALD-DNA stimulation. ( A ) Naïve B cells were incubated with ALD-DNA (50 µg/ml) in the presence or absence of LPS ranging from 0.01 µg/mL to 10 µg/ml for 72 h. The numbers of CD86-expressing B cells after each treatment were determined by flow cytometry. ( B ) Naive B cells were stimulated with ALD-DNA or E. coli single-stranded (ss) DNA (both at 50 µg/ml) for 72 h. CD86 and MHC class II expression was determined by performing flow cytometry. Histogram plots show the mean fluorescence intensity (MFI) of these surface molecules. The data are representative of three independent experiments.

    Article Snippet: CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences).

    Techniques: Expressing, Incubation, Flow Cytometry, Cytometry, Fluorescence

    Enhancement of LPS-stimulated B cell survival and division by ALD-DNA. ( A ) CFSE-labeled B cells were stimulated with ALD-DNA (50 µg/ml) in the presence or absence of LPS (100 ng/ml) for 72 h. Division progression of B cells was determined by dilution of CFSE. Representative histogram plots show the percentage of proliferating (CFSE-low) B cells after each treatment (mean values ±SEM, n = 6). Shaded areas show the cell division peaks predicted by the ModFit software. ( B ) Flow cytometry dot plots showing the gate used to select living cells and eliminate cell debris, and showing the viability of B cells (mean values ±SEM, n = 11) under different culture conditions as described above for 72 h. ( C ) Nude mice were administered one intravenous injection of indicated stimuli at day 1 and an additional intraperitoneal injection of BrdU 24 h before sacrifice. After 72 h, spleens were collected, and B220 + B cells with incorporated BrdU combined with total DNA content (with 7-AAD) were analyzed by FACS. The region gates applied to the 7-AAD versus BrdU dot plot indicate the cell subsets that were resided in the S phase of the cell cycle. Results represent 6 mice/experimental group from two separate experiments.

    Journal: PLoS ONE

    Article Title: Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production

    doi: 10.1371/journal.pone.0109095

    Figure Lengend Snippet: Enhancement of LPS-stimulated B cell survival and division by ALD-DNA. ( A ) CFSE-labeled B cells were stimulated with ALD-DNA (50 µg/ml) in the presence or absence of LPS (100 ng/ml) for 72 h. Division progression of B cells was determined by dilution of CFSE. Representative histogram plots show the percentage of proliferating (CFSE-low) B cells after each treatment (mean values ±SEM, n = 6). Shaded areas show the cell division peaks predicted by the ModFit software. ( B ) Flow cytometry dot plots showing the gate used to select living cells and eliminate cell debris, and showing the viability of B cells (mean values ±SEM, n = 11) under different culture conditions as described above for 72 h. ( C ) Nude mice were administered one intravenous injection of indicated stimuli at day 1 and an additional intraperitoneal injection of BrdU 24 h before sacrifice. After 72 h, spleens were collected, and B220 + B cells with incorporated BrdU combined with total DNA content (with 7-AAD) were analyzed by FACS. The region gates applied to the 7-AAD versus BrdU dot plot indicate the cell subsets that were resided in the S phase of the cell cycle. Results represent 6 mice/experimental group from two separate experiments.

    Article Snippet: CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences).

    Techniques: Labeling, Software, Flow Cytometry, Cytometry, Mouse Assay, Injection, FACS

    Enhancement of CD40-stimulated naïve B cell proliferation by ALD-DNA. ( A ) Naïve B cells were pre-incubated for 20 min with polymyxin B (PMB; 20 µg/ml) before the addition of ALD-DNA (50 µg/ml), LPS (100 ng/ml), anti-CD40 (1 µg/ml), LPS (100 ng/ml) plus ALD-DNA (50 µg/ml), or anti-CD40 (1 µg/ml) plus ALD-DNA (50 µg/ml). After 72 h stimulation, cell proliferation was detected by performing BrdU incorporation assay using ELISA. Data are presented as the means ±SEM of three independent experiments. ( B ) CFSE-labeled naïve B cells were cultured in media containing ALD-DNA, anti-CD40, or both (at the concentrations as described above) for 72 h. Cell division was monitored by measuring the dilution of CFSE. Shaded areas show the cell division peaks predicted by the ModFit software. Results from one representative experiment of three are shown. ** P

    Journal: PLoS ONE

    Article Title: Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production

    doi: 10.1371/journal.pone.0109095

    Figure Lengend Snippet: Enhancement of CD40-stimulated naïve B cell proliferation by ALD-DNA. ( A ) Naïve B cells were pre-incubated for 20 min with polymyxin B (PMB; 20 µg/ml) before the addition of ALD-DNA (50 µg/ml), LPS (100 ng/ml), anti-CD40 (1 µg/ml), LPS (100 ng/ml) plus ALD-DNA (50 µg/ml), or anti-CD40 (1 µg/ml) plus ALD-DNA (50 µg/ml). After 72 h stimulation, cell proliferation was detected by performing BrdU incorporation assay using ELISA. Data are presented as the means ±SEM of three independent experiments. ( B ) CFSE-labeled naïve B cells were cultured in media containing ALD-DNA, anti-CD40, or both (at the concentrations as described above) for 72 h. Cell division was monitored by measuring the dilution of CFSE. Shaded areas show the cell division peaks predicted by the ModFit software. Results from one representative experiment of three are shown. ** P

    Article Snippet: CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences).

    Techniques: Incubation, BrdU Incorporation Assay, Enzyme-linked Immunosorbent Assay, Labeling, Cell Culture, Software

    Synergistic enhancement of LPS-driven IgG production in naive B cells by ALD-DNA stimulation. Naive B cells were cultured for 6 days in media containing ALD-DNA (50 µg/ml) with or without LPS (100 ng/ml). Cell culture supernatants were collected, and the levels of IgM and IgG were measured by ELISA. Bars represent the means ±SEM of three independent experiments (n = 5). ND (not detected). ** P

    Journal: PLoS ONE

    Article Title: Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production

    doi: 10.1371/journal.pone.0109095

    Figure Lengend Snippet: Synergistic enhancement of LPS-driven IgG production in naive B cells by ALD-DNA stimulation. Naive B cells were cultured for 6 days in media containing ALD-DNA (50 µg/ml) with or without LPS (100 ng/ml). Cell culture supernatants were collected, and the levels of IgM and IgG were measured by ELISA. Bars represent the means ±SEM of three independent experiments (n = 5). ND (not detected). ** P

    Article Snippet: CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    Effect of ALD-DNA on CD138, Blimp-1, XBP1, and IL-6 expression in naïve B cells. Naïve B cells were cultured in media containing ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as described below. ( A ) After 72 h stimulation, Cells were analyzed for B220 and CD138 surface expression by flow cytometry. The number indicates the percentage of B220 − CD138 + cells in the gate. Similar results were obtained in three independent experiments. ( B ) Expression of Blimp-1 and XBP1 mRNA from B cells cultured for 96 hours was examined by real-time PCR. Fold induction relative to their untreated controls is shown (means ±SEM, n = 5). ( C ) Expression of IL-6 mRNA in B cells after 6 h stimulation was examined by real-time PCR. Fold induction relative to their untreated controls is shown (means ±SEM, n = 5); The amounts of IL-6 protein in the culture supernatants of B cells after 72 h stimulation were measured by ELISA (n = 6, a line linked two dots represents a pair observation). Up-regulation of IL-6 protein was undetectable in all samples after ALD-DNA treatment. * P

    Journal: PLoS ONE

    Article Title: Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production

    doi: 10.1371/journal.pone.0109095

    Figure Lengend Snippet: Effect of ALD-DNA on CD138, Blimp-1, XBP1, and IL-6 expression in naïve B cells. Naïve B cells were cultured in media containing ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as described below. ( A ) After 72 h stimulation, Cells were analyzed for B220 and CD138 surface expression by flow cytometry. The number indicates the percentage of B220 − CD138 + cells in the gate. Similar results were obtained in three independent experiments. ( B ) Expression of Blimp-1 and XBP1 mRNA from B cells cultured for 96 hours was examined by real-time PCR. Fold induction relative to their untreated controls is shown (means ±SEM, n = 5). ( C ) Expression of IL-6 mRNA in B cells after 6 h stimulation was examined by real-time PCR. Fold induction relative to their untreated controls is shown (means ±SEM, n = 5); The amounts of IL-6 protein in the culture supernatants of B cells after 72 h stimulation were measured by ELISA (n = 6, a line linked two dots represents a pair observation). Up-regulation of IL-6 protein was undetectable in all samples after ALD-DNA treatment. * P

    Article Snippet: CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences).

    Techniques: Expressing, Cell Culture, Flow Cytometry, Cytometry, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay