escherichia coli bl21 Search Results


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  • 94
    ATCC e coli bl21
    SDS–PAGE analysis of glutathione-S-transferase (GST)-tagged recombinant Apl_avian β-defensin (Apl_AvBD) proteins expressed in E. coli <t>BL21</t> (DE3) cells. (A) total protein from BL21 containing Apl_AvBD1, 3, 5, 6, 16, and GST with IPTG induction, respectively; (B) inclusion bodies with Apl_AvBD1, 3, 5, 6, 16, and GST respectively; (C) purified proteins of Apl_AvBD1, 3, 5, 6, 16, and GST with IPTG induction, respectively. IPTG, isopropyl-beta-D-thiogalactoside.
    E Coli Bl21, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 98 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore e coli bl21
    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli <t>BL21</t> (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.
    E Coli Bl21, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 10711 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher escherichia coli e coli bl21
    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli <t>BL21</t> (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.
    Escherichia Coli E Coli Bl21, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore bl21 e coli
    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli <t>BL21</t> (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.
    Bl21 E Coli, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 284 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli bl21 de3
    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli <t>BL21</t> (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.
    E Coli Bl21 De3, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore bl21 de3 e coli
    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli <t>BL21</t> (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.
    Bl21 De3 E Coli, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 326 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Stratagene escherichia coli e coli bl21 codonplus
    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli <t>BL21</t> (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.
    Escherichia Coli E Coli Bl21 Codonplus, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC escherichia coli strain bl21
    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli <t>BL21</t> (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.
    Escherichia Coli Strain Bl21, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    SDS–PAGE analysis of glutathione-S-transferase (GST)-tagged recombinant Apl_avian β-defensin (Apl_AvBD) proteins expressed in E. coli BL21 (DE3) cells. (A) total protein from BL21 containing Apl_AvBD1, 3, 5, 6, 16, and GST with IPTG induction, respectively; (B) inclusion bodies with Apl_AvBD1, 3, 5, 6, 16, and GST respectively; (C) purified proteins of Apl_AvBD1, 3, 5, 6, 16, and GST with IPTG induction, respectively. IPTG, isopropyl-beta-D-thiogalactoside.

    Journal: PLoS ONE

    Article Title: Identification, Expression and Activity Analyses of Five Novel Duck Beta-Defensins

    doi: 10.1371/journal.pone.0047743

    Figure Lengend Snippet: SDS–PAGE analysis of glutathione-S-transferase (GST)-tagged recombinant Apl_avian β-defensin (Apl_AvBD) proteins expressed in E. coli BL21 (DE3) cells. (A) total protein from BL21 containing Apl_AvBD1, 3, 5, 6, 16, and GST with IPTG induction, respectively; (B) inclusion bodies with Apl_AvBD1, 3, 5, 6, 16, and GST respectively; (C) purified proteins of Apl_AvBD1, 3, 5, 6, 16, and GST with IPTG induction, respectively. IPTG, isopropyl-beta-D-thiogalactoside.

    Article Snippet: Antibacterial Activity Colony counting assays were performed according to the methods used in previous studies to investigate the antimicrobial activities of glutathione-S-transferase (GST) and the recombinant AvBDs (rAvBDs), and synthetic AvBDs (sAvBDs) from ducks against four strains of Gram-positive bacteria: Micrococcus tetragenus (ATCC2835), Lactobacillus (ATCC33222), Staphylococcus aureus (ATCC 29213), and Bacillus cereus (ATCC 9193); and eight strains of Gram-negative bacteria: Bordetella bronchiseptica (S80103), Proteus mirabilis (ATCC29245), Pseudomonas aeruginosa (ATCC 9027), Pasteurella multocida (ATCC 6529), E. coli BL21 (DE3), Salmonella pullorum (C79-11-S11), Salmonella choleraesuis (CVCC 2140), and Salmonella enteritidis (ATCC 3021).

    Techniques: SDS Page, Recombinant, Purification

    Liquid extraction surface analysis (LESA) mass spectra of seven bacterial strains: Escherichia coli K-12, Escherichia coli BL21 mCherry, Pseudomonas aeruginosa PS1054, Staphylococcus aureus MSSA476, Streptococcus pneumoniae D39, Streptococcus oralis ATCC 35037, and Streptococcus gordonii ATCC 35105. All mass spectra were acquired immediately following incubation of the colonies at 37 °C, with use of the 40:60:1 acetonitrile–water–formic acid solvent system for Gram-negative bacteria and the 50:45:5 variant for Gram-positive bacteria. The streptococci were incubated under semianaerobic conditions for optimum growth; the remaining strains were grown in open air

    Journal: Journal of the American Society for Mass Spectrometry

    Article Title: Top-Down LESA Mass Spectrometry Protein Analysis of Gram-Positive and Gram-Negative Bacteria

    doi: 10.1007/s13361-017-1718-8

    Figure Lengend Snippet: Liquid extraction surface analysis (LESA) mass spectra of seven bacterial strains: Escherichia coli K-12, Escherichia coli BL21 mCherry, Pseudomonas aeruginosa PS1054, Staphylococcus aureus MSSA476, Streptococcus pneumoniae D39, Streptococcus oralis ATCC 35037, and Streptococcus gordonii ATCC 35105. All mass spectra were acquired immediately following incubation of the colonies at 37 °C, with use of the 40:60:1 acetonitrile–water–formic acid solvent system for Gram-negative bacteria and the 50:45:5 variant for Gram-positive bacteria. The streptococci were incubated under semianaerobic conditions for optimum growth; the remaining strains were grown in open air

    Article Snippet: General Overview of the Mass Spectra Figure shows representative mass spectra of seven bacterial colonies corresponding to seven strains: Gram-negative E. coli K-12, E. coli BL21 mCherry, and P. aeruginosa PS1054, and Gram-positive S. aureus MSSA476, S. pneumoniae D39, S. oralis ATCC 35037, and S. gordonii ATCC 35105.

    Techniques: Incubation, Variant Assay

    Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli BL21 (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.

    Journal: Theranostics

    Article Title: A novel HPV16 E7-affitoxin for targeted therapy of HPV16-induced human cervical cancer

    doi: 10.7150/thno.24607

    Figure Lengend Snippet: Expression and purification of Z HPV16 E7 affitoxin 384 protein. The pET21a (+)/Z HPV16 E7 affitoxin384 plasmid was transformed into E. coli BL21 (DE3). The protein was expressed and purified by Ni-NTA agarose affinity chromatography. (A) Schematic structure of pET21a (+)/Z HPV16 E7 affitoxin384 plasmid. (B) Comassie blue-stained SDS-PAGE gel of the recombinant proteins. M, protein marker; 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid; 4-5, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 6, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin; 7-8, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG. (C) Analysis of the purified Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by SDS-PAGE. M, protein marker; 1, Z HPV16 E7 affitoxin384; 2, Z wt affitoxin. (D-F) Confirmation of the expression of Z HPV16 E7 affitoxin384 and Z wt affitoxin recombinant proteins by western blot using the primary antibodies against His tag, PE38KDEL and SPA-Z, respectively. 1, Empty E.coli BL21 (DE3); 2, E.coli BL21 (DE3) transformed with pET21a empty vector; 3, E.coli BL21 (DE3) transformed with pET21a (+)/Z HPV16 E7 affitoxin384 plasmid and induced by 1 mM IPTG; 4, E.coli BL21 (DE3) transformed with pET21a (+)/Z wt affitoxin and induced by 1 mM IPTG.

    Article Snippet: The pET21a(+) vector and E.coli BL21 (DE3) were purchased from Novagen and ATCC, respectively.

    Techniques: Expressing, Purification, Plasmid Preparation, Transformation Assay, Affinity Chromatography, Staining, SDS Page, Recombinant, Marker, Western Blot

    Recombinant AfIlv3A expression, purification and DHAD activity. A. Expression of recombinant Ilv3A protein. E. coli BL21 DE3 cells were transformed with pET30_Ilv3A, grown until OD600 > 0.5 and then incubated in the presence and absence of 0.5 mM IPTG for 20 h at 20°C. Ilv3A was purified from the bacterial lysate by immobilized metal ion affinity chromatography (IMAC). Protein fractions were separated by polyacrylamide gel electrophoresis on 4–12% Bis-Tris NuPage gels (Invitrogen) followed by staining with GelCode Blue staining reagent (Pierce). The molecular weights in kilo Daltons of marker proteins are indicated. Lane 1, E. coli cell lysate; lane 2, E. coli cell lysate following IPTG induction; lane 3, Ilv3A protein purified by IMAC. B. Dihydroxyacid dehydratase activity of recombinant Ilv3A. 2 µg Ilv3A was assayed in the presence of 0–60 mM L-threonic acid. Initial reaction velocities were calculated and plotted versus substrate concentration.

    Journal: PLoS ONE

    Article Title: The Aspergillus fumigatus Dihydroxyacid Dehydratase Ilv3A/IlvC Is Required for Full Virulence

    doi: 10.1371/journal.pone.0043559

    Figure Lengend Snippet: Recombinant AfIlv3A expression, purification and DHAD activity. A. Expression of recombinant Ilv3A protein. E. coli BL21 DE3 cells were transformed with pET30_Ilv3A, grown until OD600 > 0.5 and then incubated in the presence and absence of 0.5 mM IPTG for 20 h at 20°C. Ilv3A was purified from the bacterial lysate by immobilized metal ion affinity chromatography (IMAC). Protein fractions were separated by polyacrylamide gel electrophoresis on 4–12% Bis-Tris NuPage gels (Invitrogen) followed by staining with GelCode Blue staining reagent (Pierce). The molecular weights in kilo Daltons of marker proteins are indicated. Lane 1, E. coli cell lysate; lane 2, E. coli cell lysate following IPTG induction; lane 3, Ilv3A protein purified by IMAC. B. Dihydroxyacid dehydratase activity of recombinant Ilv3A. 2 µg Ilv3A was assayed in the presence of 0–60 mM L-threonic acid. Initial reaction velocities were calculated and plotted versus substrate concentration.

    Article Snippet: Sequencing confirmed that the resulting vector, pET30_Ilv3A, was correctly constructed. pET30_Ilv3A was transformed into E. coli BL21 DE3 (Novagen) and expression was induced by IPTG as directed in the manufacturers protocol.

    Techniques: Recombinant, Expressing, Purification, Activity Assay, Transformation Assay, Incubation, Affinity Chromatography, Polyacrylamide Gel Electrophoresis, Staining, Marker, Concentration Assay

    Approved purified proteins in pLysS and E. coli BL21 (DE3) (Line 1: Marker, Line 2: pLysS, Line 3:DE3)

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Heterologous expression of a truncated form of human recombinant vascular endothelial growth factor-A and its biological activity in wound healing

    doi: 10.22038/IJBMS.2017.9011

    Figure Lengend Snippet: Approved purified proteins in pLysS and E. coli BL21 (DE3) (Line 1: Marker, Line 2: pLysS, Line 3:DE3)

    Article Snippet: E. coli DH5α (Stratagene, La Jolla, Calif) as the primary host cell and E. coli BL21 (DE3) and E. coli BL21 (DE3) pLysS (Novagen, USA) as competent cells were used.

    Techniques: Purification, Marker