escherichia coli Atcc Search Results


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  • 99
    ATCC e coli atcc 25922
    Antibacterial activity of AgNPs against different bacteria ( E .  coli  ATCC 25922,  S. aureus  ATCC 25923,  B. subtilis ,  P. aeruginosa ,  K. pneumoniae  AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.
    E Coli Atcc 25922, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 10767 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli
    Representative digital images of the colony counting plates with Staphylococcus aureus ATCC 12600 showing the anti-microbial activity against planktonic bacteria without ( a ) and with the P2-functionalized plasmonic paper ( b ); The bacterial growth inhibition rates for ( c ) planktonic bacteria and ( d ) bacterial biofilm determined for the as-designed paper-based nanoplatform when applied to both Staphylococcus aureus ATCC 12600 and <t>Escherichia</t> coli ATCC 25922 bacterial cultures.
    Escherichia Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli atcc 35218
    Assessment of viable cells harvested from biofilms of  E. coli  ATCC 35218,  S. aureus  ATCC 43387,  K. pneumoniae  6/4 and  C. albicans  ATCC 10231 after 30 min of treatment with compounds  1 ,  3 ,  4 , and  8  at their relative MIC values. Data represent the mean ± SD of cfu/mL values obtained in three independent experiments performed in duplicate. Asterisks represent values statistically significant (*  p
    E Coli Atcc 35218, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1043 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC escherichia coli atcc 8739
    Scanning electron microscopic images of tested bacteria when treated with lipid fractions at ½ minimum inhibitory concentrations (MICs). <t>Escherichia</t> coli ATCC 8739: a) control, b) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Bacillus cereus ATCC 11778: c) control, d) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Vibrio alginolyticus ATCC 17749: e) control, f) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Vibrio parahaemolyticus ATCC 17802: g) control, h) treated with ethanolic extract of lipid fraction of coriander at γ =500 µg/mL; and Listeria monocytogenes ATCC 13932: i) control, j) treated with ethanolic extract of lipid fraction of bay leaf at γ =500 µg/mL
    Escherichia Coli Atcc 8739, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 537 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli atcc 10536
    Effect of pH (2.5) in E. coli <t>ATCC</t> 10536 ( A , D ); S. Typhi ( B , E ) and K. pneumoniae ( C , F ) survival after the fourth passage of adaptation at ¼ × MIC ( A – C ) and the eighth until ½ × MIC ( D – F ) of branded and unbranded honey. ( p > 0.05); * ( p
    E Coli Atcc 10536, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 198 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli o157 h7 atcc 35150
    Effect of lactic acid, succinic acid and phenyllactic acid on foodborne pathogenic bacterial growth. E. coli <t>O157:H7</t> ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were incubated with the different concentration of lactic acid (A) , succinic acid (B) or phenyllactic acid (C) at 37°C for 24 h. The bacterial growth was determined at OD 600 . The growth rate of foodborne pathogenic bacteria without lactic acid, succinic acid or phenyllactic acid was assigned to 100% (Control).
    E Coli O157 H7 Atcc 35150, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 239 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli atcc 11775
    Chromosome (A) and plasmid (B) comparison of assembled Escherichia coli <t>ATCC</t> 11775 results between this study and that of Meier-Kolthoff et al. ( 1 ). From the outside in, (i) the assembled genome from this study (with the genome coordinate), (ii) ONT read coverage depth plot (light blue), (iii) rRNA and tRNA locations (blue), (iv) positive-strand open reading frame (ORF) locations (green), (v) negative-strand ORF locations (red), (vi) assembled genome from Meier-Kolthoff et al. ( 1 ) (orange), and (vii) GC-skew plot of the assembled genome from this study (light gray represents G-rich [positive value], dark gray represents non-G-rich [negative value]). The three overlap points of the contigs are shown in the p, q, and r locations. (C) Integrative Genomics Viewer (IGV) snapshots show that ONT reads span across the contigs of the GCF_000690815.1 genome on the repeat locations of p, q, and r as shown in panel A. Top, chromosome location; middle, ONT read alignment; bottom, GCF_000690815.1 contigs.
    E Coli Atcc 11775, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 177 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli o157 h7 atcc 43895
    The determination of stx1 and stx2 genes of E. coli <t>O157</t> and O157:H7 by multiplex PCR. Lane M: Marker (100~1,000 bp), Lane K: positive control ( E. coli O157:H7 ATCC 43895).
    E Coli O157 H7 Atcc 43895, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli atcc 11229
    NCT, NVC-422, and NVC-612 inhibit the cytopathic effect of Stx2 in Vero cells. Supernatants of EHEC 178 cultures were treated with 1.38–55 mM of oxidants for 30 min (A–D), diluted 100-fold in RPMI + 10% v/v FCS, and added to Vero cell cultures, which were monitored for the typical cytopathic effect. (A–C) Numbers of cytopathic cells per visual field at a 100x magnification after incubation with EHEC supernatant for 72 h. Comparison of RPMI + FCS without Stx (negative control), supernatant of the non-EHEC strain <t>ATCC</t> 11229 (shown in A), untreated supernatant (positive control), and supernatant treated with NCT (A), NVC-422 (B), or NVC-612 (C). (D) Quantification of cell death by LDH assay after incubation with EHEC supernatant. Values were related to untreated supernatant. An additional separate positive control was performed with 1% Igepal. (E) Supernatants of EHEC 178 cultures were treated with 55 mM NCT for 1–30 min, followed by Vero cell assay and evaluation as in A–C. Mean values ± SD from three independent experiments are shown in (A–E). *P
    E Coli Atcc 11229, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC escherichia coli atcc 11105
    NCT, NVC-422, and NVC-612 inhibit the cytopathic effect of Stx2 in Vero cells. Supernatants of EHEC 178 cultures were treated with 1.38–55 mM of oxidants for 30 min (A–D), diluted 100-fold in RPMI + 10% v/v FCS, and added to Vero cell cultures, which were monitored for the typical cytopathic effect. (A–C) Numbers of cytopathic cells per visual field at a 100x magnification after incubation with EHEC supernatant for 72 h. Comparison of RPMI + FCS without Stx (negative control), supernatant of the non-EHEC strain <t>ATCC</t> 11229 (shown in A), untreated supernatant (positive control), and supernatant treated with NCT (A), NVC-422 (B), or NVC-612 (C). (D) Quantification of cell death by LDH assay after incubation with EHEC supernatant. Values were related to untreated supernatant. An additional separate positive control was performed with 1% Igepal. (E) Supernatants of EHEC 178 cultures were treated with 55 mM NCT for 1–30 min, followed by Vero cell assay and evaluation as in A–C. Mean values ± SD from three independent experiments are shown in (A–E). *P
    Escherichia Coli Atcc 11105, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC e coli atcc 33456
    Effects of cinnamaldehyde (a), eugenol (b), and citronellol (c) on the specific growth rate of E. coli <t>ATCC</t> 33456 and selected Pseudomonas strains.
    E Coli Atcc 33456, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    ATCC escherichia coli atcc 11303
    Allele-specific PCRs to detect G88A and G88 pbp3 alleles in S. aureus . Allele-specific PCR assays that detect the USA300 G88A (A) and non-USA300 G88 (B) pbp3 sequences were performed, and gel electrophoresis on a 1% agarose gel was used to detect amplification for the following bacterial strains: lanes 1 and 14, Affymetrix 1-kb Plus ladder; lane 2, HA-MRSA, SCC mec I, BAA-38; lane 3, HA-MRSA, SCC mec II, 0158p; lane 4, HA-MRSA, SCC mec III, BAA-39; lane 5, CA-MRSA, SCC mec IV, BAA-1556; lane 6, Streptococcus agalactiae A909; lane 7, Streptococcus agalactiae NEM316; lane 8, Streptococcus agalactiae O90R; lane 9, methicillin-susceptible S. aureus ATCC 29213; lane 10, Streptococcus pyogenes ATCC 19615; lane 11, <t>Escherichia</t> coli ATCC 11303; lane 12, Staphylococcus epidermidis ATCC 12228; lane 13, no-template control.
    Escherichia Coli Atcc 11303, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    ATCC e coli o157 h7 atcc 43888
    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli <t>O157:H7</t> ATCC 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.
    E Coli O157 H7 Atcc 43888, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 189 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    ATCC e coli atcc 29522
    Effects of 80 AU ml −1  of purified antimicrobial peptide AN5-1 produced by  P. alvei  AN5 on early exponential growth phase against target strains:  a E. coli  ATCC 29522,  b S. marcescens ,  c S. aureus  and  d B. cereus  ATCC 14579, in the absence ( filled circle ) and presence ( filled triangle ) of antimicrobial peptide AN5-1. The bacterial growth was measured by means of optical density at 600 nm (OD 600 )
    E Coli Atcc 29522, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli atcc 13706
    A plaque isolation plate, as described in section Materials and methods with typical plaques visible on host strain <t>ATCC</t> 13706
    E Coli Atcc 13706, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Antibacterial activity of AgNPs against different bacteria ( E .  coli  ATCC 25922,  S. aureus  ATCC 25923,  B. subtilis ,  P. aeruginosa ,  K. pneumoniae  AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

    Journal: Scientific Reports

    Article Title: Shape dependent physical mutilation and lethal effects of silver nanoparticles on bacteria

    doi: 10.1038/s41598-017-18590-6

    Figure Lengend Snippet: Antibacterial activity of AgNPs against different bacteria ( E . coli ATCC 25922, S. aureus ATCC 25923, B. subtilis , P. aeruginosa , K. pneumoniae AWD5) at different concentrations of ( A ) AgNP-sp and ( B ) AgNR. 255, 249, 242 and 230 µg assigned as S0, S1, S2 and S3 for AgNP-sp and 399, 392, 380, 364 µg for AgNR were assigned as R0, R1, R2 and R3 respectively.

    Article Snippet: AgNR had been reported to have similar effects against E . coil , though we found it for four other bacteria along with E. coli ATCC 25922.

    Techniques: Activity Assay

    Intracellular DnaK concentration (A) and surviving-cell counts (B) of E. coli ATCC 25922 cells exposed to heat treatment of various intensities ( F 70 10 ). The concentrations per cell were calculated with the number of cells present before the treatment. (A) Horizontal line indicates the DnaK level found in cells during exponential growth (OD 600 = 0.5) at 37°C (38,500 molecules/cell). (B) Horizontal line indicates the cell count detection limit. The experiment was repeated three times. Bars represent the mean plus or minus standard deviation. For a specific process lethality value, results with a similar lowercase letter are not significantly different. Capital letters are used for a specific temperature, i.e., AB for 50°C and XYZ for 55°C. Results below the detection level are indicated by an asterisk.

    Journal: Applied and Environmental Microbiology

    Article Title: Escherichia coli Heat Shock Protein DnaK: Production and Consequences in Terms of Monitoring Cooking

    doi: 10.1128/AEM.69.6.3231-3237.2003

    Figure Lengend Snippet: Intracellular DnaK concentration (A) and surviving-cell counts (B) of E. coli ATCC 25922 cells exposed to heat treatment of various intensities ( F 70 10 ). The concentrations per cell were calculated with the number of cells present before the treatment. (A) Horizontal line indicates the DnaK level found in cells during exponential growth (OD 600 = 0.5) at 37°C (38,500 molecules/cell). (B) Horizontal line indicates the cell count detection limit. The experiment was repeated three times. Bars represent the mean plus or minus standard deviation. For a specific process lethality value, results with a similar lowercase letter are not significantly different. Capital letters are used for a specific temperature, i.e., AB for 50°C and XYZ for 55°C. Results below the detection level are indicated by an asterisk.

    Article Snippet: Overall, the intracellular DnaK concentration in E. coli strain ATCC 25922 was highly correlated ( P < 0.0001) to the number of surviving cells, but the relationship was only poorly described ( R 2 = 0.6881) by a linear regression (Fig. ).

    Techniques: Concentration Assay, Cell Counting, Standard Deviation

    (A) Mouse survival curves representing the toxicity of ASB (50 to 400 mg/kg). We used 10 mice for each group. (B) Mouse survival curves representing the therapeutic effects of MEM (2.5 mg/kg) or ASB (100 mg/kg) used alone or in combination. Mice were intraperitoneally injected with SMB-1-producing E. coli ( E. coli ATCC 25922 carrying pCL-SMB-1) (10 7 CFU). We used 10 mice for each group. The groups were analyzed using a Mantel-Cox test.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: 4-Amino-2-Sulfanylbenzoic Acid as a Potent Subclass B3 Metallo-β-Lactamase-Specific Inhibitor Applicable for Distinguishing Metallo-β-Lactamase Subclasses

    doi: 10.1128/AAC.01197-19

    Figure Lengend Snippet: (A) Mouse survival curves representing the toxicity of ASB (50 to 400 mg/kg). We used 10 mice for each group. (B) Mouse survival curves representing the therapeutic effects of MEM (2.5 mg/kg) or ASB (100 mg/kg) used alone or in combination. Mice were intraperitoneally injected with SMB-1-producing E. coli ( E. coli ATCC 25922 carrying pCL-SMB-1) (10 7 CFU). We used 10 mice for each group. The groups were analyzed using a Mantel-Cox test.

    Article Snippet: Plasmid pCL-SMB-1 ( ) was introduced into the E. coli ATCC 25922 strain, after which the resulting strain was used for the animal experiments.

    Techniques: Mouse Assay, Injection

    In vitro dynamic time-kill curves using human exposures of moxalactam, cefotaxime, and cefoperazone/sulbactam against Escherichia coli ATCC25922 and CTX-M-producing E. coli and Klebsiella pneumoniae . Notes: ( A ) and ( B ) show simulated dosing regimens against E. coli ATCC25922; ( C ) and ( D ) show simulated dosing regimens against E. coli 3376; ( E ) and ( F ) show simulated dosing regimens against K. pneumoniae 2689. The dotted lines indicate the regimens of MOX. The solid lines indicate the regimens of CTX and CFZ/SBT. The lower limit of detection (broken line) was 1.47 log 10 CFU/mL. Abbreviations: ATCC, American Type Culture Collection; CFU, colony forming units; CFZ/SBT, cefoperazone/sulbactam; CTX, cefotaxime; MOX, moxalactam; qd, once-daily; q12h, twice-daily; q8h, thrice-daily.

    Journal: Infection and Drug Resistance

    Article Title: Antibacterial effect evaluation of moxalactam against extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae with in vitro pharmacokinetics/pharmacodynamics simulation

    doi: 10.2147/IDR.S150431

    Figure Lengend Snippet: In vitro dynamic time-kill curves using human exposures of moxalactam, cefotaxime, and cefoperazone/sulbactam against Escherichia coli ATCC25922 and CTX-M-producing E. coli and Klebsiella pneumoniae . Notes: ( A ) and ( B ) show simulated dosing regimens against E. coli ATCC25922; ( C ) and ( D ) show simulated dosing regimens against E. coli 3376; ( E ) and ( F ) show simulated dosing regimens against K. pneumoniae 2689. The dotted lines indicate the regimens of MOX. The solid lines indicate the regimens of CTX and CFZ/SBT. The lower limit of detection (broken line) was 1.47 log 10 CFU/mL. Abbreviations: ATCC, American Type Culture Collection; CFU, colony forming units; CFZ/SBT, cefoperazone/sulbactam; CTX, cefotaxime; MOX, moxalactam; qd, once-daily; q12h, twice-daily; q8h, thrice-daily.

    Article Snippet: Two clinical ESBL-producing strains (blaCTX-M-15 positive E. coli 3376 and blaCTX-M-14 positive K. pneumoniae 2689) and E. coli American Type Culture Collection (ATCC)25922 were used in the study.

    Techniques: In Vitro

    The relationship between %T > MIC of MOX, CTX and CFZ/SBT against the tested bacteria and IE. Note: Three isolates are denoted by different symbols. Abbreviations: ATCC, American Type Culture Collection; CFU, colony forming units; CFZ, cefoperazone; CTX, cefotaxime; E. coli , Escherichia coli ; IE, the area between the control growth and bacterial killing and regrowth curves; K. pneumoniae , Klebsiella pneumoniae ; MIC, minimum inhibitory concentration; MOX, moxalactam; SBT, sulbactam; %T > MIC, the time during which the concentration of the drug remained above the MIC.

    Journal: Infection and Drug Resistance

    Article Title: Antibacterial effect evaluation of moxalactam against extended-spectrum β-lactamase-producing Escherichia coli and Klebsiella pneumoniae with in vitro pharmacokinetics/pharmacodynamics simulation

    doi: 10.2147/IDR.S150431

    Figure Lengend Snippet: The relationship between %T > MIC of MOX, CTX and CFZ/SBT against the tested bacteria and IE. Note: Three isolates are denoted by different symbols. Abbreviations: ATCC, American Type Culture Collection; CFU, colony forming units; CFZ, cefoperazone; CTX, cefotaxime; E. coli , Escherichia coli ; IE, the area between the control growth and bacterial killing and regrowth curves; K. pneumoniae , Klebsiella pneumoniae ; MIC, minimum inhibitory concentration; MOX, moxalactam; SBT, sulbactam; %T > MIC, the time during which the concentration of the drug remained above the MIC.

    Article Snippet: Two clinical ESBL-producing strains (blaCTX-M-15 positive E. coli 3376 and blaCTX-M-14 positive K. pneumoniae 2689) and E. coli American Type Culture Collection (ATCC)25922 were used in the study.

    Techniques: Concentration Assay

    Characterization of the nwSlide as an AST platform. (A) Photograph of a nwSlide (25 mm × 75 mm) holding 672 nanowells in a 14 × 48 matrix. (B) Side view of one nanowell with dimensions and volume (V) indicated. (C) Growth measured at OD 600 of a wt Escherichia coli laboratory strain (W3110, black) and a strain with mutated fnr gene ( BW25113Δfnr , blue) in a nwSlide. Circa 200 nanowells were recorded for each strain, dashed lines = SD, n = 3. (D) The design of functionalized nwSlides used for nwASTs. The left side offers 24 non-functionalized nanowells each for negative (NEG., medium only) and positive (POS., inoculated medium) controls of bacterial growth. The antibiotics ampicillin (blue, AMP), ciprofloxacin (green, CIP), nitrofurantoin (red, NIT), cefadroxil (purple, CFR), mecillinam (yellow, MEC), and trimethoprim (brown, TMP) are coated and distributed in separate rows. The antibiotic concentration varies from lowest (left) to highest (right) as indicated schematically above the nwSlide. Each antibiotic is represented by seven twofold dilutions. Each concentration includes four nanowells that serve as technical replicates. Antibiotic concentrations in individual experiments are defined in Section “Materials and Methods.” (E) MIC determination of the reference strain E. coli ATCC 25922 from one nwAST functionalized as in (D) . The heatmap shows OD 600 recordings in each of the 216 nanocultures over 12 h at indicated conditions. A color change from yellow (low OD 600 ) to red (high OD 600 ) in one row indicates bacterial growth in the corresponding nanowell. Negative and positive controls include 24 wells each. For each antibiotic, growth pattern of the 28 nanocultures at 7 antibiotic concentrations is shown. The vertical gradient symbol indicates that nanocultures in the upper rows are exposed to the lowest concentration of antibiotics, whereas a gradual increase leaves the lower rows representing nanowells exposed to the highest concentration. Black dots represent the T lag of each nanoculture.

    Journal: Frontiers in Microbiology

    Article Title: Rapid Phenotypic Antibiotic Susceptibility Testing of Uropathogens Using Optical Signal Analysis on the Nanowell Slide

    doi: 10.3389/fmicb.2018.01530

    Figure Lengend Snippet: Characterization of the nwSlide as an AST platform. (A) Photograph of a nwSlide (25 mm × 75 mm) holding 672 nanowells in a 14 × 48 matrix. (B) Side view of one nanowell with dimensions and volume (V) indicated. (C) Growth measured at OD 600 of a wt Escherichia coli laboratory strain (W3110, black) and a strain with mutated fnr gene ( BW25113Δfnr , blue) in a nwSlide. Circa 200 nanowells were recorded for each strain, dashed lines = SD, n = 3. (D) The design of functionalized nwSlides used for nwASTs. The left side offers 24 non-functionalized nanowells each for negative (NEG., medium only) and positive (POS., inoculated medium) controls of bacterial growth. The antibiotics ampicillin (blue, AMP), ciprofloxacin (green, CIP), nitrofurantoin (red, NIT), cefadroxil (purple, CFR), mecillinam (yellow, MEC), and trimethoprim (brown, TMP) are coated and distributed in separate rows. The antibiotic concentration varies from lowest (left) to highest (right) as indicated schematically above the nwSlide. Each antibiotic is represented by seven twofold dilutions. Each concentration includes four nanowells that serve as technical replicates. Antibiotic concentrations in individual experiments are defined in Section “Materials and Methods.” (E) MIC determination of the reference strain E. coli ATCC 25922 from one nwAST functionalized as in (D) . The heatmap shows OD 600 recordings in each of the 216 nanocultures over 12 h at indicated conditions. A color change from yellow (low OD 600 ) to red (high OD 600 ) in one row indicates bacterial growth in the corresponding nanowell. Negative and positive controls include 24 wells each. For each antibiotic, growth pattern of the 28 nanocultures at 7 antibiotic concentrations is shown. The vertical gradient symbol indicates that nanocultures in the upper rows are exposed to the lowest concentration of antibiotics, whereas a gradual increase leaves the lower rows representing nanowells exposed to the highest concentration. Black dots represent the T lag of each nanoculture.

    Article Snippet: Bacterial Strains, Media, and Antibiotics Strains included in this study were wild type (wt) Escherichia coli laboratory strain W3110 , BW25113 (Δfnr-771::kan) [National BioResource Project (NIG, Japan):E. coli ], and the reference strain E. coli ATCC 25922 (Oxoid, United Kingdom).

    Techniques: AST Assay, Concentration Assay

    Photolabeling of E. coli ATCC 25922 with thanatin-PAL5. ( A ) Western blot (biotin detection) and corresponding SDS–polyacrylamide gel electrophoresis (SDS-PAGE) (Coomassie blue staining) of membrane protein fraction from: lane 1, control unlabeled cells; lanes 2 and 3, cells photolabeled with thanatin-PAL5 (10 and 2 μg/ml); and lane 4, cells photolabeled with thanatin-PAL5 (10 μg/ml) + competitor thanatin (200 μg/ml). ( B ) Western blot and SDS-PAGE after photolabeling with thanatin-PAL5 (2 μg/ml) with (+) or without (−) reduction of extracted membrane proteins with dithiothreitol (DTT). ( C ) Volcano plot showing relative abundance of E. coli proteins in thanatin-PAL5 labeled versus unlabeled control sample after streptavidin pulldown detected by MS-based proteomic analysis. Significantly enriched proteins (right/above dashed lines) are highlighted in green and represent PAL5-labeled proteins.

    Journal: Science Advances

    Article Title: Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli

    doi: 10.1126/sciadv.aau2634

    Figure Lengend Snippet: Photolabeling of E. coli ATCC 25922 with thanatin-PAL5. ( A ) Western blot (biotin detection) and corresponding SDS–polyacrylamide gel electrophoresis (SDS-PAGE) (Coomassie blue staining) of membrane protein fraction from: lane 1, control unlabeled cells; lanes 2 and 3, cells photolabeled with thanatin-PAL5 (10 and 2 μg/ml); and lane 4, cells photolabeled with thanatin-PAL5 (10 μg/ml) + competitor thanatin (200 μg/ml). ( B ) Western blot and SDS-PAGE after photolabeling with thanatin-PAL5 (2 μg/ml) with (+) or without (−) reduction of extracted membrane proteins with dithiothreitol (DTT). ( C ) Volcano plot showing relative abundance of E. coli proteins in thanatin-PAL5 labeled versus unlabeled control sample after streptavidin pulldown detected by MS-based proteomic analysis. Significantly enriched proteins (right/above dashed lines) are highlighted in green and represent PAL5-labeled proteins.

    Article Snippet: E. coli ATCC 25922 cells grown in MH-I broth (50 ml) to an OD600 (optical density at 600 nm) of 1.0 were collected, washed once, taken up in phosphate-buffered saline (PBS) (50 ml), and incubated for 30 min at 37°C with shaking at 200 rpm in the dark with thanatin-PAL5 (2 to 10 μg/ml).

    Techniques: Western Blot, Polyacrylamide Gel Electrophoresis, SDS Page, Staining, Labeling, Mass Spectrometry

    Electron and fluorescence microscopy studies. ( A and B ) TEM studies of E. coli ATCC 25922, before (A) and after (B) thanatin treatment (1.5 μg/ml), showing internal accumulations of membrane-like material. Scale bars, 500 nm. ( C and D ) Super-resolution fluorescence microscopy of E. coli ATCC 25922 without (C) or with thanatin (5 μg/ml) (D) and stained with FM4-64, SYTOX Green, or DAPI. Top: The FM4-64 channel (red staining). Bottom: Superimposition of all three channels [with DAPI (blue) and SYTOX Green (nondetected)]. ( E ) E. coli staining with thanatin-BDP-FL (8 μg/ml) for 2 hours at 30°C (both pictures). Cells were analyzed using a Leica CLSM SP8 gSTED microscope. Scale bars, 4 or 10 μm (bottom right). For experimental details, see section S5.

    Journal: Science Advances

    Article Title: Thanatin targets the intermembrane protein complex required for lipopolysaccharide transport in Escherichia coli

    doi: 10.1126/sciadv.aau2634

    Figure Lengend Snippet: Electron and fluorescence microscopy studies. ( A and B ) TEM studies of E. coli ATCC 25922, before (A) and after (B) thanatin treatment (1.5 μg/ml), showing internal accumulations of membrane-like material. Scale bars, 500 nm. ( C and D ) Super-resolution fluorescence microscopy of E. coli ATCC 25922 without (C) or with thanatin (5 μg/ml) (D) and stained with FM4-64, SYTOX Green, or DAPI. Top: The FM4-64 channel (red staining). Bottom: Superimposition of all three channels [with DAPI (blue) and SYTOX Green (nondetected)]. ( E ) E. coli staining with thanatin-BDP-FL (8 μg/ml) for 2 hours at 30°C (both pictures). Cells were analyzed using a Leica CLSM SP8 gSTED microscope. Scale bars, 4 or 10 μm (bottom right). For experimental details, see section S5.

    Article Snippet: E. coli ATCC 25922 cells grown in MH-I broth (50 ml) to an OD600 (optical density at 600 nm) of 1.0 were collected, washed once, taken up in phosphate-buffered saline (PBS) (50 ml), and incubated for 30 min at 37°C with shaking at 200 rpm in the dark with thanatin-PAL5 (2 to 10 μg/ml).

    Techniques: Fluorescence, Microscopy, Transmission Electron Microscopy, Staining, Confocal Laser Scanning Microscopy

    Dependence of the SI for E. coli ATCC 25922 cells on the concentration of the silica particle suspensions for amino–/silica (a) and amino–/phenyl–/silica (b) samples (yellow − control, red − 60 min, green − 120 min).

    Journal: ACS Omega

    Article Title: Diverse Pathway to Obtain Antibacterial and Antifungal Agents Based on Silica Particles Functionalized by Amino and Phenyl Groups with Cu(II) Ion Complexes

    doi: 10.1021/acsomega.0c01335

    Figure Lengend Snippet: Dependence of the SI for E. coli ATCC 25922 cells on the concentration of the silica particle suspensions for amino–/silica (a) and amino–/phenyl–/silica (b) samples (yellow − control, red − 60 min, green − 120 min).

    Article Snippet: Against the cells of the strain E. coli ATCC 25922, the most effective of all analyzed Cu(II) + NH2 –/C6 H5 –/SiO2 concentrations were 1.0 and 0.001%, for which practically no viable cells remained (SI = 0.5 and 0.3%) ( ).

    Techniques: Concentration Assay

    Representative results of the antifungal tests against ( a ) Candida albicans ATCC 10231; ( b ) Escherichia coli ATCC 25922; and ( c ) Staphylococcus aureus ATCC 25923 after 17 h of incubation with samples of the PMMA resin and composites previously stored in distilled water.

    Journal: Materials

    Article Title: Effect of Storage in Distilled Water for Three Months on the Antimicrobial Properties of Poly(methyl methacrylate) Denture Base Material Doped with Inorganic Filler

    doi: 10.3390/ma9050328

    Figure Lengend Snippet: Representative results of the antifungal tests against ( a ) Candida albicans ATCC 10231; ( b ) Escherichia coli ATCC 25922; and ( c ) Staphylococcus aureus ATCC 25923 after 17 h of incubation with samples of the PMMA resin and composites previously stored in distilled water.

    Article Snippet: The following standard strains of microorganisms were used: gram-positive Staphylococcus aureus ATCC 25923 (S. aureus ), gram-negative Escherichia coli ATCC 25922 (E. coli ) and the yeast-type fungus Candida albicans ATCC 10231 (C. albicans ).

    Techniques: Incubation

    In vitro activity of linezolid alone and combined with aztreonam or ceftazidime against E. coli ATCC 25922 (a) and a clinical isolate (b).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Evaluating Aztreonam and Ceftazidime Pharmacodynamics with Escherichia coli in Combination with Daptomycin, Linezolid, or Vancomycin in an In Vitro Pharmacodynamic Model

    doi: 10.1128/AAC.00180-09

    Figure Lengend Snippet: In vitro activity of linezolid alone and combined with aztreonam or ceftazidime against E. coli ATCC 25922 (a) and a clinical isolate (b).

    Article Snippet: Indifference was noted at 24 h. Daptomycin, vancomycin, and linezolid monotherapy demonstrated no significant activity against E. coli ATCC 25922 at any time point.

    Techniques: In Vitro, Activity Assay

    In vitro activity of daptomycin alone and combined with aztreonam or ceftazidime against E. coli ATCC 25922 (a) and a clinical isolate (b). Results show log 10 numbers of CFU/ml of activity ± standard deviations.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Evaluating Aztreonam and Ceftazidime Pharmacodynamics with Escherichia coli in Combination with Daptomycin, Linezolid, or Vancomycin in an In Vitro Pharmacodynamic Model

    doi: 10.1128/AAC.00180-09

    Figure Lengend Snippet: In vitro activity of daptomycin alone and combined with aztreonam or ceftazidime against E. coli ATCC 25922 (a) and a clinical isolate (b). Results show log 10 numbers of CFU/ml of activity ± standard deviations.

    Article Snippet: Indifference was noted at 24 h. Daptomycin, vancomycin, and linezolid monotherapy demonstrated no significant activity against E. coli ATCC 25922 at any time point.

    Techniques: In Vitro, Activity Assay

    In vitro activity of vancomycin alone and combined with aztreonam or ceftazidime against E. coli ATCC 25922 (a) and a clinical isolate (b).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Evaluating Aztreonam and Ceftazidime Pharmacodynamics with Escherichia coli in Combination with Daptomycin, Linezolid, or Vancomycin in an In Vitro Pharmacodynamic Model

    doi: 10.1128/AAC.00180-09

    Figure Lengend Snippet: In vitro activity of vancomycin alone and combined with aztreonam or ceftazidime against E. coli ATCC 25922 (a) and a clinical isolate (b).

    Article Snippet: Indifference was noted at 24 h. Daptomycin, vancomycin, and linezolid monotherapy demonstrated no significant activity against E. coli ATCC 25922 at any time point.

    Techniques: In Vitro, Activity Assay

    Representative digital images of the colony counting plates with Staphylococcus aureus ATCC 12600 showing the anti-microbial activity against planktonic bacteria without ( a ) and with the P2-functionalized plasmonic paper ( b ); The bacterial growth inhibition rates for ( c ) planktonic bacteria and ( d ) bacterial biofilm determined for the as-designed paper-based nanoplatform when applied to both Staphylococcus aureus ATCC 12600 and Escherichia coli ATCC 25922 bacterial cultures.

    Journal: Molecules

    Article Title: Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform

    doi: 10.3390/molecules25143182

    Figure Lengend Snippet: Representative digital images of the colony counting plates with Staphylococcus aureus ATCC 12600 showing the anti-microbial activity against planktonic bacteria without ( a ) and with the P2-functionalized plasmonic paper ( b ); The bacterial growth inhibition rates for ( c ) planktonic bacteria and ( d ) bacterial biofilm determined for the as-designed paper-based nanoplatform when applied to both Staphylococcus aureus ATCC 12600 and Escherichia coli ATCC 25922 bacterial cultures.

    Article Snippet: 1 mL of the Staphylococcus aureus and Escherichia coli diluted suspensions were placed in each of a series of 25 mL sterile glass tubes.

    Techniques: Activity Assay, Inhibition

    Assessment of viable cells harvested from biofilms of  E. coli  ATCC 35218,  S. aureus  ATCC 43387,  K. pneumoniae  6/4 and  C. albicans  ATCC 10231 after 30 min of treatment with compounds  1 ,  3 ,  4 , and  8  at their relative MIC values. Data represent the mean ± SD of cfu/mL values obtained in three independent experiments performed in duplicate. Asterisks represent values statistically significant (*  p

    Journal: Microorganisms

    Article Title: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

    doi: 10.3390/microorganisms7020028

    Figure Lengend Snippet: Assessment of viable cells harvested from biofilms of E. coli ATCC 35218, S. aureus ATCC 43387, K. pneumoniae 6/4 and C. albicans ATCC 10231 after 30 min of treatment with compounds 1 , 3 , 4 , and 8 at their relative MIC values. Data represent the mean ± SD of cfu/mL values obtained in three independent experiments performed in duplicate. Asterisks represent values statistically significant (* p

    Article Snippet: As regards compound 3 , the lowest MIC value (64 µg/mL) was evidenced for E. coli ATCC 35218 and 128 µg/mL for all others species.

    Techniques:

    Disaggregating efficacy of compounds  1 ,  3 ,  4 , and  8  at their relative MIC values, against biofilms of  E. coli  ATCC 35218,  S. aureus  ATCC 43387,  K. pneumoniae  6/4 and  C. albicans  ATCC 10231, as assessed by spectrophotometer reader (OD  570nm ) after 30 min of contact. Data represent the mean ± SD obtained in three independent experiments performed in duplicate. Asterisks represent values statistically significant (*  p

    Journal: Microorganisms

    Article Title: Marine Alkaloid 2,2-Bis(6-bromo-3-indolyl) Ethylamine and Its Synthetic Derivatives Inhibit Microbial Biofilms Formation and Disaggregate Developed Biofilms

    doi: 10.3390/microorganisms7020028

    Figure Lengend Snippet: Disaggregating efficacy of compounds 1 , 3 , 4 , and 8 at their relative MIC values, against biofilms of E. coli ATCC 35218, S. aureus ATCC 43387, K. pneumoniae 6/4 and C. albicans ATCC 10231, as assessed by spectrophotometer reader (OD 570nm ) after 30 min of contact. Data represent the mean ± SD obtained in three independent experiments performed in duplicate. Asterisks represent values statistically significant (* p

    Article Snippet: As regards compound 3 , the lowest MIC value (64 µg/mL) was evidenced for E. coli ATCC 35218 and 128 µg/mL for all others species.

    Techniques: Spectrophotometry

    Scanning electron microscopic images of tested bacteria when treated with lipid fractions at ½ minimum inhibitory concentrations (MICs). Escherichia coli ATCC 8739: a) control, b) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Bacillus cereus ATCC 11778: c) control, d) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Vibrio alginolyticus ATCC 17749: e) control, f) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Vibrio parahaemolyticus ATCC 17802: g) control, h) treated with ethanolic extract of lipid fraction of coriander at γ =500 µg/mL; and Listeria monocytogenes ATCC 13932: i) control, j) treated with ethanolic extract of lipid fraction of bay leaf at γ =500 µg/mL

    Journal: Food Technology and Biotechnology

    Article Title: Time-Kill Kinetics of Lipid Fractions Isolated from Condiments against Foodborne Pathogens

    doi: 10.17113/ftb.56.02.18.

    Figure Lengend Snippet: Scanning electron microscopic images of tested bacteria when treated with lipid fractions at ½ minimum inhibitory concentrations (MICs). Escherichia coli ATCC 8739: a) control, b) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Bacillus cereus ATCC 11778: c) control, d) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Vibrio alginolyticus ATCC 17749: e) control, f) treated with ethanolic extract of lipid fraction of black cumin at γ =125 µg/mL; Vibrio parahaemolyticus ATCC 17802: g) control, h) treated with ethanolic extract of lipid fraction of coriander at γ =500 µg/mL; and Listeria monocytogenes ATCC 13932: i) control, j) treated with ethanolic extract of lipid fraction of bay leaf at γ =500 µg/mL

    Article Snippet: Escherichia coli ATCC 8739, Listeria monocytogenes ATCC 13932, Vibrio parahaemolyticus ATCC 17802, Bacillus cereus ATCC 11778 and Vibrio alginolyticus ATCC 17749 are the most important pathogens of great concern to food industry.

    Techniques:

    Effect of different lipid fractions on the viability of Escherichia coli ATCC 8739. The vertical bars represent the standard error of mean values of three replicates. MN, MC, MB, EN, EC and EB stand for methanolic and ethanolic extracts of lipid fractions of black cumin, coriander seeds and bay leaf, respectively, at their minimum inhibitory concentrations (MICs)

    Journal: Food Technology and Biotechnology

    Article Title: Time-Kill Kinetics of Lipid Fractions Isolated from Condiments against Foodborne Pathogens

    doi: 10.17113/ftb.56.02.18.

    Figure Lengend Snippet: Effect of different lipid fractions on the viability of Escherichia coli ATCC 8739. The vertical bars represent the standard error of mean values of three replicates. MN, MC, MB, EN, EC and EB stand for methanolic and ethanolic extracts of lipid fractions of black cumin, coriander seeds and bay leaf, respectively, at their minimum inhibitory concentrations (MICs)

    Article Snippet: Escherichia coli ATCC 8739, Listeria monocytogenes ATCC 13932, Vibrio parahaemolyticus ATCC 17802, Bacillus cereus ATCC 11778 and Vibrio alginolyticus ATCC 17749 are the most important pathogens of great concern to food industry.

    Techniques:

    Effect of pH (2.5) in E. coli ATCC 10536 ( A , D ); S. Typhi ( B , E ) and K. pneumoniae ( C , F ) survival after the fourth passage of adaptation at ¼ × MIC ( A – C ) and the eighth until ½ × MIC ( D – F ) of branded and unbranded honey. ( p > 0.05); * ( p

    Journal: Foods

    Article Title: Antibiotics, Acid and Heat Tolerance of Honey adapted Escherichia coli, Salmonella Typhi and Klebsiella pneumoniae

    doi: 10.3390/foods9030311

    Figure Lengend Snippet: Effect of pH (2.5) in E. coli ATCC 10536 ( A , D ); S. Typhi ( B , E ) and K. pneumoniae ( C , F ) survival after the fourth passage of adaptation at ¼ × MIC ( A – C ) and the eighth until ½ × MIC ( D – F ) of branded and unbranded honey. ( p > 0.05); * ( p

    Article Snippet: Similarly, MICs of unbranded honey did not change for adapted and unadapted cells of E. coli ATCC 10536 (25% v/v ), S. Typhi (25% v/v ) and K. pneumoniae (25% v/v ).

    Techniques:

    Effect of different concentrations of branded honey (Marhaba) ( A – C ) and unbranded honey (extracted from Ziziphus mauritiana plant) ( D – F ) on bacterial growth (OD 600nm ) of E. coli ATCC 10536 ( A , D ), S. Typhi ( B , E ) and K. pneumoniae ( C , F ).

    Journal: Foods

    Article Title: Antibiotics, Acid and Heat Tolerance of Honey adapted Escherichia coli, Salmonella Typhi and Klebsiella pneumoniae

    doi: 10.3390/foods9030311

    Figure Lengend Snippet: Effect of different concentrations of branded honey (Marhaba) ( A – C ) and unbranded honey (extracted from Ziziphus mauritiana plant) ( D – F ) on bacterial growth (OD 600nm ) of E. coli ATCC 10536 ( A , D ), S. Typhi ( B , E ) and K. pneumoniae ( C , F ).

    Article Snippet: Similarly, MICs of unbranded honey did not change for adapted and unadapted cells of E. coli ATCC 10536 (25% v/v ), S. Typhi (25% v/v ) and K. pneumoniae (25% v/v ).

    Techniques:

    Effect of temperature (60 °C) in E. coli ATCC 10536 ( A , D ); S. Typhi ( B , E ) and K. pneumoniae ( C , F ) survival after the fourth passage of adaptation at ¼ × MIC ( A – C ) and the eighth until ½ × MIC ( D – F ) of branded and unbranded honey. ( p > 0.05); * ( p

    Journal: Foods

    Article Title: Antibiotics, Acid and Heat Tolerance of Honey adapted Escherichia coli, Salmonella Typhi and Klebsiella pneumoniae

    doi: 10.3390/foods9030311

    Figure Lengend Snippet: Effect of temperature (60 °C) in E. coli ATCC 10536 ( A , D ); S. Typhi ( B , E ) and K. pneumoniae ( C , F ) survival after the fourth passage of adaptation at ¼ × MIC ( A – C ) and the eighth until ½ × MIC ( D – F ) of branded and unbranded honey. ( p > 0.05); * ( p

    Article Snippet: Similarly, MICs of unbranded honey did not change for adapted and unadapted cells of E. coli ATCC 10536 (25% v/v ), S. Typhi (25% v/v ) and K. pneumoniae (25% v/v ).

    Techniques:

    Effect of lactic acid, succinic acid and phenyllactic acid on foodborne pathogenic bacterial growth. E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were incubated with the different concentration of lactic acid (A) , succinic acid (B) or phenyllactic acid (C) at 37°C for 24 h. The bacterial growth was determined at OD 600 . The growth rate of foodborne pathogenic bacteria without lactic acid, succinic acid or phenyllactic acid was assigned to 100% (Control).

    Journal: Frontiers in Microbiology

    Article Title: Antagonistic Activities and Probiotic Potential of Lactic Acid Bacteria Derived From a Plant-Based Fermented Food

    doi: 10.3389/fmicb.2018.01963

    Figure Lengend Snippet: Effect of lactic acid, succinic acid and phenyllactic acid on foodborne pathogenic bacterial growth. E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were incubated with the different concentration of lactic acid (A) , succinic acid (B) or phenyllactic acid (C) at 37°C for 24 h. The bacterial growth was determined at OD 600 . The growth rate of foodborne pathogenic bacteria without lactic acid, succinic acid or phenyllactic acid was assigned to 100% (Control).

    Article Snippet: After incubation, the HT-29 cells were washed with phosphate-buffered saline (PBS) and lysed with the addition of 0.2% Triton X-100 or 0.25% trypsin-EDTA for 10 min. Then, the viable cells of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were determined by plating the appropriate agar plates as follows: MacConkey agar for the count of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021 or Salmonella Typhimurium KCTC 1925, and Baird Parker agar supplemented with 5% egg yolk for the count of S. aureus KCCM 11335.

    Techniques: Incubation, Concentration Assay

    Inhibition of foodborne pathogenic bacterial growth in the presence of CFS from the LAB strains. Escherichia coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) or Staphylococcus aureus KCCM 11335 (D) were incubated in the presence of CFS or neutralized CFS (pH 6.5) from the LAB strains ( Lactobacillus curvatus KCCM 43119, Leuconostoc mesenteroides KCCM 43060, Weissella cibaria KCTC 3746, or W. koreensis KCCM 41517) at 37°C for 24 h. The bacterial growth was determined at OD 600 . The growth rate of foodborne pathogenic bacteria without CFS was assigned to 100% (Control).

    Journal: Frontiers in Microbiology

    Article Title: Antagonistic Activities and Probiotic Potential of Lactic Acid Bacteria Derived From a Plant-Based Fermented Food

    doi: 10.3389/fmicb.2018.01963

    Figure Lengend Snippet: Inhibition of foodborne pathogenic bacterial growth in the presence of CFS from the LAB strains. Escherichia coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) or Staphylococcus aureus KCCM 11335 (D) were incubated in the presence of CFS or neutralized CFS (pH 6.5) from the LAB strains ( Lactobacillus curvatus KCCM 43119, Leuconostoc mesenteroides KCCM 43060, Weissella cibaria KCTC 3746, or W. koreensis KCCM 41517) at 37°C for 24 h. The bacterial growth was determined at OD 600 . The growth rate of foodborne pathogenic bacteria without CFS was assigned to 100% (Control).

    Article Snippet: After incubation, the HT-29 cells were washed with phosphate-buffered saline (PBS) and lysed with the addition of 0.2% Triton X-100 or 0.25% trypsin-EDTA for 10 min. Then, the viable cells of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were determined by plating the appropriate agar plates as follows: MacConkey agar for the count of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021 or Salmonella Typhimurium KCTC 1925, and Baird Parker agar supplemented with 5% egg yolk for the count of S. aureus KCCM 11335.

    Techniques: Inhibition, Incubation

    Changes in adhesion of E. coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) , or S. aureus KCCM 11335 (D) to HT-29 cells. HT-29 cells were co-treated with foodborne pathogenic bacteria and the LAB strains ( L. curvatus KCCM 43119, Ln. mesenteroides KCCM 43060, W. cibaria KCTC 3746, or W. koreensis KCCM 41517) for 1 h and the adhesion of foodborne pathogenic bacteria was determined. The adhesion of foodborne pathogenic bacteria alone to HT-29 cells was assigned to 100% (control). An asterisk ( ∗ ) indicates the statistical significance compared with control ( P

    Journal: Frontiers in Microbiology

    Article Title: Antagonistic Activities and Probiotic Potential of Lactic Acid Bacteria Derived From a Plant-Based Fermented Food

    doi: 10.3389/fmicb.2018.01963

    Figure Lengend Snippet: Changes in adhesion of E. coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) , or S. aureus KCCM 11335 (D) to HT-29 cells. HT-29 cells were co-treated with foodborne pathogenic bacteria and the LAB strains ( L. curvatus KCCM 43119, Ln. mesenteroides KCCM 43060, W. cibaria KCTC 3746, or W. koreensis KCCM 41517) for 1 h and the adhesion of foodborne pathogenic bacteria was determined. The adhesion of foodborne pathogenic bacteria alone to HT-29 cells was assigned to 100% (control). An asterisk ( ∗ ) indicates the statistical significance compared with control ( P

    Article Snippet: After incubation, the HT-29 cells were washed with phosphate-buffered saline (PBS) and lysed with the addition of 0.2% Triton X-100 or 0.25% trypsin-EDTA for 10 min. Then, the viable cells of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were determined by plating the appropriate agar plates as follows: MacConkey agar for the count of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021 or Salmonella Typhimurium KCTC 1925, and Baird Parker agar supplemented with 5% egg yolk for the count of S. aureus KCCM 11335.

    Techniques:

    Changes in adhesion of E. coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) , or S. aureus KCCM 11335 (D) to HT-29 cells. HT-29 cells were pre-treated with the LAB strains ( L. curvatus KCCM 43119, Ln. mesenteroides KCCM 43060, W. cibaria KCTC 3746, or W. koreensis KCCM 41517). After 1 h, the HT-29 cells were treated with the foodborne pathogenic bacteria for an additional 1 h and the adhesion of foodborne pathogenic bacteria was determined. The adhesion of foodborne pathogenic bacteria alone to HT-29 cells was assigned to 100% (control). An asterisk ( ∗ ) indicates the statistical significance compared with control ( P

    Journal: Frontiers in Microbiology

    Article Title: Antagonistic Activities and Probiotic Potential of Lactic Acid Bacteria Derived From a Plant-Based Fermented Food

    doi: 10.3389/fmicb.2018.01963

    Figure Lengend Snippet: Changes in adhesion of E. coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) , or S. aureus KCCM 11335 (D) to HT-29 cells. HT-29 cells were pre-treated with the LAB strains ( L. curvatus KCCM 43119, Ln. mesenteroides KCCM 43060, W. cibaria KCTC 3746, or W. koreensis KCCM 41517). After 1 h, the HT-29 cells were treated with the foodborne pathogenic bacteria for an additional 1 h and the adhesion of foodborne pathogenic bacteria was determined. The adhesion of foodborne pathogenic bacteria alone to HT-29 cells was assigned to 100% (control). An asterisk ( ∗ ) indicates the statistical significance compared with control ( P

    Article Snippet: After incubation, the HT-29 cells were washed with phosphate-buffered saline (PBS) and lysed with the addition of 0.2% Triton X-100 or 0.25% trypsin-EDTA for 10 min. Then, the viable cells of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were determined by plating the appropriate agar plates as follows: MacConkey agar for the count of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021 or Salmonella Typhimurium KCTC 1925, and Baird Parker agar supplemented with 5% egg yolk for the count of S. aureus KCCM 11335.

    Techniques:

    Changes in adhesion of E. coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) , or S. aureus KCCM 11335 (D) to HT-29 cells. HT-29 cells were pre-treated with foodborne pathogenic bacteria. After 1 h, the HT-29 cells were treated with the LAB strains ( L. curvatus KCCM 43119, Ln. mesenteroides KCCM 43060, W. cibaria KCTC 3746, or W. koreensis KCCM 41517) for an additional 1 h and the adhesion of foodborne pathogenic bacteria was determined. The adhesion of foodborne pathogenic bacteria alone to HT-29 cells was assigned to 100% (control). An asterisk ( ∗ ) indicates the statistical significance compared with control ( P

    Journal: Frontiers in Microbiology

    Article Title: Antagonistic Activities and Probiotic Potential of Lactic Acid Bacteria Derived From a Plant-Based Fermented Food

    doi: 10.3389/fmicb.2018.01963

    Figure Lengend Snippet: Changes in adhesion of E. coli O157:H7 ATCC 35150 (A) , Salmonella Enteritidis KCCM 12021 (B) , Salmonella Typhimurium KCTC 1925 (C) , or S. aureus KCCM 11335 (D) to HT-29 cells. HT-29 cells were pre-treated with foodborne pathogenic bacteria. After 1 h, the HT-29 cells were treated with the LAB strains ( L. curvatus KCCM 43119, Ln. mesenteroides KCCM 43060, W. cibaria KCTC 3746, or W. koreensis KCCM 41517) for an additional 1 h and the adhesion of foodborne pathogenic bacteria was determined. The adhesion of foodborne pathogenic bacteria alone to HT-29 cells was assigned to 100% (control). An asterisk ( ∗ ) indicates the statistical significance compared with control ( P

    Article Snippet: After incubation, the HT-29 cells were washed with phosphate-buffered saline (PBS) and lysed with the addition of 0.2% Triton X-100 or 0.25% trypsin-EDTA for 10 min. Then, the viable cells of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021, Salmonella Typhimurium KCTC 1925, or S. aureus KCCM 11335 were determined by plating the appropriate agar plates as follows: MacConkey agar for the count of E. coli O157:H7 ATCC 35150, Salmonella Enteritidis KCCM 12021 or Salmonella Typhimurium KCTC 1925, and Baird Parker agar supplemented with 5% egg yolk for the count of S. aureus KCCM 11335.

    Techniques:

    Chromosome (A) and plasmid (B) comparison of assembled Escherichia coli ATCC 11775 results between this study and that of Meier-Kolthoff et al. ( 1 ). From the outside in, (i) the assembled genome from this study (with the genome coordinate), (ii) ONT read coverage depth plot (light blue), (iii) rRNA and tRNA locations (blue), (iv) positive-strand open reading frame (ORF) locations (green), (v) negative-strand ORF locations (red), (vi) assembled genome from Meier-Kolthoff et al. ( 1 ) (orange), and (vii) GC-skew plot of the assembled genome from this study (light gray represents G-rich [positive value], dark gray represents non-G-rich [negative value]). The three overlap points of the contigs are shown in the p, q, and r locations. (C) Integrative Genomics Viewer (IGV) snapshots show that ONT reads span across the contigs of the GCF_000690815.1 genome on the repeat locations of p, q, and r as shown in panel A. Top, chromosome location; middle, ONT read alignment; bottom, GCF_000690815.1 contigs.

    Journal: Microbiology Resource Announcements

    Article Title: Complete Genome and Plasmid Sequences of Escherichia coli Type Strain ATCC 11775

    doi: 10.1128/MRA.00046-19

    Figure Lengend Snippet: Chromosome (A) and plasmid (B) comparison of assembled Escherichia coli ATCC 11775 results between this study and that of Meier-Kolthoff et al. ( 1 ). From the outside in, (i) the assembled genome from this study (with the genome coordinate), (ii) ONT read coverage depth plot (light blue), (iii) rRNA and tRNA locations (blue), (iv) positive-strand open reading frame (ORF) locations (green), (v) negative-strand ORF locations (red), (vi) assembled genome from Meier-Kolthoff et al. ( 1 ) (orange), and (vii) GC-skew plot of the assembled genome from this study (light gray represents G-rich [positive value], dark gray represents non-G-rich [negative value]). The three overlap points of the contigs are shown in the p, q, and r locations. (C) Integrative Genomics Viewer (IGV) snapshots show that ONT reads span across the contigs of the GCF_000690815.1 genome on the repeat locations of p, q, and r as shown in panel A. Top, chromosome location; middle, ONT read alignment; bottom, GCF_000690815.1 contigs.

    Article Snippet: We present here the complete genome sequence for E. coli ATCC 11775.

    Techniques: Plasmid Preparation

    The determination of stx1 and stx2 genes of E. coli O157 and O157:H7 by multiplex PCR. Lane M: Marker (100~1,000 bp), Lane K: positive control ( E. coli O157:H7 ATCC 43895).

    Journal: Journal of Veterinary Science

    Article Title: Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey

    doi: 10.4142/jvs.2010.11.4.321

    Figure Lengend Snippet: The determination of stx1 and stx2 genes of E. coli O157 and O157:H7 by multiplex PCR. Lane M: Marker (100~1,000 bp), Lane K: positive control ( E. coli O157:H7 ATCC 43895).

    Article Snippet: E. coli O157:H7 ATCC 43895 was used as a reference strain.

    Techniques: Multiplex Assay, Polymerase Chain Reaction, Marker, Positive Control

    NCT, NVC-422, and NVC-612 inhibit the cytopathic effect of Stx2 in Vero cells. Supernatants of EHEC 178 cultures were treated with 1.38–55 mM of oxidants for 30 min (A–D), diluted 100-fold in RPMI + 10% v/v FCS, and added to Vero cell cultures, which were monitored for the typical cytopathic effect. (A–C) Numbers of cytopathic cells per visual field at a 100x magnification after incubation with EHEC supernatant for 72 h. Comparison of RPMI + FCS without Stx (negative control), supernatant of the non-EHEC strain ATCC 11229 (shown in A), untreated supernatant (positive control), and supernatant treated with NCT (A), NVC-422 (B), or NVC-612 (C). (D) Quantification of cell death by LDH assay after incubation with EHEC supernatant. Values were related to untreated supernatant. An additional separate positive control was performed with 1% Igepal. (E) Supernatants of EHEC 178 cultures were treated with 55 mM NCT for 1–30 min, followed by Vero cell assay and evaluation as in A–C. Mean values ± SD from three independent experiments are shown in (A–E). *P

    Journal: PLoS ONE

    Article Title: N-Chlorotaurine, a Long-Lived Oxidant Produced by Human Leukocytes, Inactivates Shiga Toxin of Enterohemorrhagic Escherichia coli

    doi: 10.1371/journal.pone.0047105

    Figure Lengend Snippet: NCT, NVC-422, and NVC-612 inhibit the cytopathic effect of Stx2 in Vero cells. Supernatants of EHEC 178 cultures were treated with 1.38–55 mM of oxidants for 30 min (A–D), diluted 100-fold in RPMI + 10% v/v FCS, and added to Vero cell cultures, which were monitored for the typical cytopathic effect. (A–C) Numbers of cytopathic cells per visual field at a 100x magnification after incubation with EHEC supernatant for 72 h. Comparison of RPMI + FCS without Stx (negative control), supernatant of the non-EHEC strain ATCC 11229 (shown in A), untreated supernatant (positive control), and supernatant treated with NCT (A), NVC-422 (B), or NVC-612 (C). (D) Quantification of cell death by LDH assay after incubation with EHEC supernatant. Values were related to untreated supernatant. An additional separate positive control was performed with 1% Igepal. (E) Supernatants of EHEC 178 cultures were treated with 55 mM NCT for 1–30 min, followed by Vero cell assay and evaluation as in A–C. Mean values ± SD from three independent experiments are shown in (A–E). *P

    Article Snippet: Controls without oxidants, without EHEC supernatant, with taurine or dimethyltaurine and supernatant, with plain RPMI + FCS, and with supernatant of E. coli ATCC 11229, a Shiga toxin negative strain, were performed in parallel.

    Techniques: Incubation, Negative Control, Positive Control, Lactate Dehydrogenase Assay

    Effects of cinnamaldehyde (a), eugenol (b), and citronellol (c) on the specific growth rate of E. coli ATCC 33456 and selected Pseudomonas strains.

    Journal: Applied and Environmental Microbiology

    Article Title: Colorimetric Method for Identifying Plant Essential Oil Components That Affect Biofilm Formation and Structure

    doi: 10.1128/AEM.70.12.6951-6956.2004

    Figure Lengend Snippet: Effects of cinnamaldehyde (a), eugenol (b), and citronellol (c) on the specific growth rate of E. coli ATCC 33456 and selected Pseudomonas strains.

    Article Snippet: Although eugenol strongly inhibited the growth of E. coli ATCC 33456, the growth of the three Pseudomonas spp. was reduced by only 20% ± 5% at a eugenol concentration of 2,600 μM (425 ppm), the maximum concentration tested.

    Techniques:

    Plant essential oil toxicity for  E. coli  ATCC 33456. (a) Toxicity measured by using the specific growth rate. (b) Toxicity expressed as a percentage of growth compared to the growth in LB medium after 15 h.

    Journal: Applied and Environmental Microbiology

    Article Title: Colorimetric Method for Identifying Plant Essential Oil Components That Affect Biofilm Formation and Structure

    doi: 10.1128/AEM.70.12.6951-6956.2004

    Figure Lengend Snippet: Plant essential oil toxicity for E. coli ATCC 33456. (a) Toxicity measured by using the specific growth rate. (b) Toxicity expressed as a percentage of growth compared to the growth in LB medium after 15 h.

    Article Snippet: Although eugenol strongly inhibited the growth of E. coli ATCC 33456, the growth of the three Pseudomonas spp. was reduced by only 20% ± 5% at a eugenol concentration of 2,600 μM (425 ppm), the maximum concentration tested.

    Techniques:

    Cell viability in E. coli ATCC 33456 biofilms after 18 h of growth in the presence of plant essential oil components. Open bars, live cells; solid bars, dead cells.

    Journal: Applied and Environmental Microbiology

    Article Title: Colorimetric Method for Identifying Plant Essential Oil Components That Affect Biofilm Formation and Structure

    doi: 10.1128/AEM.70.12.6951-6956.2004

    Figure Lengend Snippet: Cell viability in E. coli ATCC 33456 biofilms after 18 h of growth in the presence of plant essential oil components. Open bars, live cells; solid bars, dead cells.

    Article Snippet: Although eugenol strongly inhibited the growth of E. coli ATCC 33456, the growth of the three Pseudomonas spp. was reduced by only 20% ± 5% at a eugenol concentration of 2,600 μM (425 ppm), the maximum concentration tested.

    Techniques:

    Allele-specific PCRs to detect G88A and G88 pbp3 alleles in S. aureus . Allele-specific PCR assays that detect the USA300 G88A (A) and non-USA300 G88 (B) pbp3 sequences were performed, and gel electrophoresis on a 1% agarose gel was used to detect amplification for the following bacterial strains: lanes 1 and 14, Affymetrix 1-kb Plus ladder; lane 2, HA-MRSA, SCC mec I, BAA-38; lane 3, HA-MRSA, SCC mec II, 0158p; lane 4, HA-MRSA, SCC mec III, BAA-39; lane 5, CA-MRSA, SCC mec IV, BAA-1556; lane 6, Streptococcus agalactiae A909; lane 7, Streptococcus agalactiae NEM316; lane 8, Streptococcus agalactiae O90R; lane 9, methicillin-susceptible S. aureus ATCC 29213; lane 10, Streptococcus pyogenes ATCC 19615; lane 11, Escherichia coli ATCC 11303; lane 12, Staphylococcus epidermidis ATCC 12228; lane 13, no-template control.

    Journal: Journal of Clinical Microbiology

    Article Title: Detection of Epidemic USA300 Community-Associated Methicillin-Resistant Staphylococcus aureus Strains by Use of a Single Allele-Specific PCR Assay Targeting a Novel Polymorphism of Staphylococcus aureus pbp3

    doi: 10.1128/JCM.00417-13

    Figure Lengend Snippet: Allele-specific PCRs to detect G88A and G88 pbp3 alleles in S. aureus . Allele-specific PCR assays that detect the USA300 G88A (A) and non-USA300 G88 (B) pbp3 sequences were performed, and gel electrophoresis on a 1% agarose gel was used to detect amplification for the following bacterial strains: lanes 1 and 14, Affymetrix 1-kb Plus ladder; lane 2, HA-MRSA, SCC mec I, BAA-38; lane 3, HA-MRSA, SCC mec II, 0158p; lane 4, HA-MRSA, SCC mec III, BAA-39; lane 5, CA-MRSA, SCC mec IV, BAA-1556; lane 6, Streptococcus agalactiae A909; lane 7, Streptococcus agalactiae NEM316; lane 8, Streptococcus agalactiae O90R; lane 9, methicillin-susceptible S. aureus ATCC 29213; lane 10, Streptococcus pyogenes ATCC 19615; lane 11, Escherichia coli ATCC 11303; lane 12, Staphylococcus epidermidis ATCC 12228; lane 13, no-template control.

    Article Snippet: Both G88 and A88 reactions were performed for CA-MRSA (strain BAA-1556), HA-MRSA (strains BAA-38, BAA-39, and 0158p), MSSA (strain 29213), Streptococcus agalactiae strains O90R, NEM316, and A909, Streptococcus pyogenes ATCC 19615, Escherichia coli ATCC 11303, and Staphylococcus epidermidis ATCC 12228 to determine the specificity of the reaction.

    Techniques: Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Agarose Gel Electrophoresis, Amplification

    (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 ATCC 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.

    Journal: Applied and Environmental Microbiology

    Article Title: Abundance in Sewage of Bacteriophages That Infect Escherichia coli O157:H7 and That Carry the Shiga Toxin 2 Gene

    doi:

    Figure Lengend Snippet: (A) Western blot (with anti-Stx 2 A subunit) of crude concentrated supernatants of cultures of E. coli O157:H7 ATCC 43888 infected with: 10, 1, and 0.1 ml of sewage that had been centrifuged, filtered, and DNase treated (lanes 2, 3, and 4, respectively); sonically derived lysates of E. coli O157:H7 ATCC 43889 (lane 1) and ATCC 43888 (lane 5); sewage that had been centrifuged, filtered, and DNase treated (lane 6); and prestained protein size standards in kDa (lane M). (B) Lanes are as in panel A, after cutting a strip of the SDS-PAGE slab gel of the zone to which the A subunit migrates, eluting the strips electrophoretically, and concentrating the eluates by passage through 10K cutoff filtration membrane microconcentrators (Microsep) by centrifugation at 16,000 × g at 4°C.

    Article Snippet: E. coli O157:H7 ATCC 43888, which does not produce either Shiga toxin 1 or 2 and does not possess the genes for these toxins, was used as a negative control in all experiments.

    Techniques: Western Blot, Infection, Derivative Assay, Stripping Membranes, SDS Page, Filtration, Centrifugation

    Visual estimation of Vero cell cytotoxicity. Wells 1 to 5 were inoculated with growth medium; well 6 was inoculated with sewage that had been centrifuged, filtered, and DNase treated; wells 7, 8, 9, and 10 were inoculated with 10-fold dilutions of a crude supernatant of E. coli O157:H7 ATCC 43888 infected with 10 ml of sewage that had been centrifuged, filtered, and DNase treated. Wells 11, 12, 13, 14, and 15 were like wells 6, 7, 8, 9, and 10, respectively, but with the addition of anti-Stx 2 subunit A MAb 11E10.

    Journal: Applied and Environmental Microbiology

    Article Title: Abundance in Sewage of Bacteriophages That Infect Escherichia coli O157:H7 and That Carry the Shiga Toxin 2 Gene

    doi:

    Figure Lengend Snippet: Visual estimation of Vero cell cytotoxicity. Wells 1 to 5 were inoculated with growth medium; well 6 was inoculated with sewage that had been centrifuged, filtered, and DNase treated; wells 7, 8, 9, and 10 were inoculated with 10-fold dilutions of a crude supernatant of E. coli O157:H7 ATCC 43888 infected with 10 ml of sewage that had been centrifuged, filtered, and DNase treated. Wells 11, 12, 13, 14, and 15 were like wells 6, 7, 8, 9, and 10, respectively, but with the addition of anti-Stx 2 subunit A MAb 11E10.

    Article Snippet: E. coli O157:H7 ATCC 43888, which does not produce either Shiga toxin 1 or 2 and does not possess the genes for these toxins, was used as a negative control in all experiments.

    Techniques: Infection

    Effects of 80 AU ml −1  of purified antimicrobial peptide AN5-1 produced by  P. alvei  AN5 on early exponential growth phase against target strains:  a E. coli  ATCC 29522,  b S. marcescens ,  c S. aureus  and  d B. cereus  ATCC 14579, in the absence ( filled circle ) and presence ( filled triangle ) of antimicrobial peptide AN5-1. The bacterial growth was measured by means of optical density at 600 nm (OD 600 )

    Journal: Journal of Industrial Microbiology & Biotechnology

    Article Title: Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5

    doi: 10.1007/s10295-013-1259-5

    Figure Lengend Snippet: Effects of 80 AU ml −1 of purified antimicrobial peptide AN5-1 produced by P. alvei AN5 on early exponential growth phase against target strains: a E. coli ATCC 29522, b S. marcescens , c S. aureus and d B. cereus ATCC 14579, in the absence ( filled circle ) and presence ( filled triangle ) of antimicrobial peptide AN5-1. The bacterial growth was measured by means of optical density at 600 nm (OD 600 )

    Article Snippet: The synthesized peptide AN5-1 was tested against E. coli ATCC 29522, and the resulting inhibition zones were 17 mm similar to the one obtained from CFCS, which confirms the active structure of the small antimicrobial peptide AN5-1.

    Techniques: Purification, Produced

    Tricine SDS-PAGE supplemented with glycerol for purified antimicrobial peptide AN5-1 produced by Paenibacillus alvei AN5. Lane 1 color marker ultra-low range (M.W. 1.06–26.6) KDa (Sigma, USA), lane 2 purified AN5-1 stained with Coomassie blue, lane 3 bacteriocin assay overlying on soft agar shows inhibitory activity of the active peptide against E. coli ATCC 29522

    Journal: Journal of Industrial Microbiology & Biotechnology

    Article Title: Detection of secreted antimicrobial peptides isolated from cell-free culture supernatant of Paenibacillus alvei AN5

    doi: 10.1007/s10295-013-1259-5

    Figure Lengend Snippet: Tricine SDS-PAGE supplemented with glycerol for purified antimicrobial peptide AN5-1 produced by Paenibacillus alvei AN5. Lane 1 color marker ultra-low range (M.W. 1.06–26.6) KDa (Sigma, USA), lane 2 purified AN5-1 stained with Coomassie blue, lane 3 bacteriocin assay overlying on soft agar shows inhibitory activity of the active peptide against E. coli ATCC 29522

    Article Snippet: The synthesized peptide AN5-1 was tested against E. coli ATCC 29522, and the resulting inhibition zones were 17 mm similar to the one obtained from CFCS, which confirms the active structure of the small antimicrobial peptide AN5-1.

    Techniques: SDS Page, Purification, Produced, Chromosome Transmission Fidelity Colony Color Assay, Staining, Activity Assay

    A plaque isolation plate, as described in section Materials and methods with typical plaques visible on host strain ATCC 13706

    Journal: European Journal of Microbiology & Immunology

    Article Title: Antimicrobial Resistance-Transducing Bacteriophages Isolated from Surfaces of Equine Surgery Clinics – A Pilot Study

    doi: 10.1556/1886.2017.00032

    Figure Lengend Snippet: A plaque isolation plate, as described in section Materials and methods with typical plaques visible on host strain ATCC 13706

    Article Snippet: E. coli ATCC 13706 was grown to an optical density of McFarland 0.5.

    Techniques: Isolation