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  • 94
    ATCC e coli e coli k 12
    E Coli E Coli K 12, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore eschericha coli e coli expression
    Eschericha Coli E Coli Expression, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Pasteur Institute escherichia coli e coli
    Escherichia Coli E Coli, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC e coli ab1157
    E Coli Ab1157, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC e coli c600
    Adhesion capacities of bacterial strains on bean seeds. X. citri pv. phaseoli var. fuscans strains CFBP4834-R, 4834HRCR, 4834HRPG, and 4834YAPH2; X. campestris pv. campestris ATCC 33913; and E. coli <t>C600</t> suspensions at 1 × 10 5 CFU ml −1
    E Coli C600, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli ml35p
    Synergy in bactericidal action. Histogram showing results for untreated and antibiotic-treated samples of E. coli <t>ML35p.</t> The antibiotic concentrations used are indicated below the bars. Cells were treated with or without drug for 18 h and plated on MH agar plates. After 20 h of incubation at 37°C, the colonies were counted. Synergy in bactericidal activity was observed for Ud and rifampin at an FBC index of 0.16. The log 10 CFU/ml observed at the end point is indicated above each bar. Standard deviations from triplicate experiments are plotted. RIF, rifampin.
    E Coli Ml35p, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC e coli dh5α
    Southern blot analysis of traC in E. coli and P. stewartii subsp. stewartii . (A) Five micrograms of DNA from E. coli <t>DH5α</t> (lane 1) and E. coli HB101 (lane 2) was digested with BglII/NotI. Lane 3 was loaded with P. stewartii subsp. stewartii SW2
    E Coli Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC e coli jm109
    EC 50 of SeNPs and TeNPs for planktonic and biofilm cultures of E. coli <t>JM109</t> (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) after 4 h of exposure and 24 h of growth + 24 h of exposure .
    E Coli Jm109, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC e coli mc1061
    Chemical structures of bulgecins (A) and monobactams (B) produced by ATCC 31363 and ATCC 31433. (C) Bulgecin extract and aztreonam display potentiation against E. coli <t>MC1061</t> in liquid culture and (D) detection of bulgecin A in culture extract. (C) E. coli
    E Coli Mc1061, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli mg1655
    AI-2 removal profile for bacteria producing AI-2. Shown is extracellular AI-2 activity in cell-free culture fluids from LuxS + strains E. coli <t>MG1655</t> (triangles) and UPEC UTI89 (circles) (A) and B. cereus (diamonds) and B. anthracis (squares) (B)
    E Coli Mg1655, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 232 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e coli o157h7
    AI-2 removal profile for bacteria producing AI-2. Shown is extracellular AI-2 activity in cell-free culture fluids from LuxS + strains E. coli <t>MG1655</t> (triangles) and UPEC UTI89 (circles) (A) and B. cereus (diamonds) and B. anthracis (squares) (B)
    E Coli O157h7, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC e coli w3110
    Analysis of IscU expression and purification. ( A ) SDS/PAGE of overproduction and purification. Lane 1, molecular weight standards; lane 2, BL-21 cells harboring pTrcIscU (25 μg of protein); lane 3, BL-21 cells harboring pTrcIscU plasmid grown for ≈16 h after IPTG induction (25 μg of protein); lanes 4–6, purified IscU. ( B ) Immunoblot analysis of constitutive IscU expression in <t>W3110,</t> DH5α, and BL-21 cells grown to stationary phase in Terrific Broth. Lanes 1–3, purified IscU; lanes 4–6, E. coli whole cells (15 μg of protein each).
    E Coli W3110, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Adhesion capacities of bacterial strains on bean seeds. X. citri pv. phaseoli var. fuscans strains CFBP4834-R, 4834HRCR, 4834HRPG, and 4834YAPH2; X. campestris pv. campestris ATCC 33913; and E. coli C600 suspensions at 1 × 10 5 CFU ml −1

    Journal: Applied and Environmental Microbiology

    Article Title: Transmission of Plant-Pathogenic Bacteria by Nonhost Seeds without Induction of an Associated Defense Reaction at Emergence ▿Transmission of Plant-Pathogenic Bacteria by Nonhost Seeds without Induction of an Associated Defense Reaction at Emergence ▿ †

    doi: 10.1128/AEM.01098-10

    Figure Lengend Snippet: Adhesion capacities of bacterial strains on bean seeds. X. citri pv. phaseoli var. fuscans strains CFBP4834-R, 4834HRCR, 4834HRPG, and 4834YAPH2; X. campestris pv. campestris ATCC 33913; and E. coli C600 suspensions at 1 × 10 5 CFU ml −1

    Article Snippet: Adhesion capacities tested in seed columns were not significantly different ( P > 0.05) among a set of strains composed of E. coli C600, X. campestris pv. campestris ATCC 33913, and 4834YAPH2, an adhesin mutant of X. citri pv. phaseoli var. fuscans in the yapH2 gene (Fig. ).

    Techniques:

    Colonization of bean seedlings by X. citri pv. phaseoli var. fuscans wild-type strain CFBP4834-R and mutant strains 4834HRCR and 4834HRPG, X. campestris pv. campestris ATCC 33913, and E. coli C600. Bacterial population sizes were quantified on bean seeds

    Journal: Applied and Environmental Microbiology

    Article Title: Transmission of Plant-Pathogenic Bacteria by Nonhost Seeds without Induction of an Associated Defense Reaction at Emergence ▿Transmission of Plant-Pathogenic Bacteria by Nonhost Seeds without Induction of an Associated Defense Reaction at Emergence ▿ †

    doi: 10.1128/AEM.01098-10

    Figure Lengend Snippet: Colonization of bean seedlings by X. citri pv. phaseoli var. fuscans wild-type strain CFBP4834-R and mutant strains 4834HRCR and 4834HRPG, X. campestris pv. campestris ATCC 33913, and E. coli C600. Bacterial population sizes were quantified on bean seeds

    Article Snippet: Adhesion capacities tested in seed columns were not significantly different ( P > 0.05) among a set of strains composed of E. coli C600, X. campestris pv. campestris ATCC 33913, and 4834YAPH2, an adhesin mutant of X. citri pv. phaseoli var. fuscans in the yapH2 gene (Fig. ).

    Techniques: Mutagenesis

    Kinetics of the adhesion capacities of bacterial strains on a polypropylene surface. X. citri pv. phaseoli var. fuscans strains CFBP4834, 4834HRCR, 4834HRPG, and 4834YAPH2; X. campestris pv. campestris ATCC 33913; and E. coli C600 were cultured during

    Journal: Applied and Environmental Microbiology

    Article Title: Transmission of Plant-Pathogenic Bacteria by Nonhost Seeds without Induction of an Associated Defense Reaction at Emergence ▿Transmission of Plant-Pathogenic Bacteria by Nonhost Seeds without Induction of an Associated Defense Reaction at Emergence ▿ †

    doi: 10.1128/AEM.01098-10

    Figure Lengend Snippet: Kinetics of the adhesion capacities of bacterial strains on a polypropylene surface. X. citri pv. phaseoli var. fuscans strains CFBP4834, 4834HRCR, 4834HRPG, and 4834YAPH2; X. campestris pv. campestris ATCC 33913; and E. coli C600 were cultured during

    Article Snippet: Adhesion capacities tested in seed columns were not significantly different ( P > 0.05) among a set of strains composed of E. coli C600, X. campestris pv. campestris ATCC 33913, and 4834YAPH2, an adhesin mutant of X. citri pv. phaseoli var. fuscans in the yapH2 gene (Fig. ).

    Techniques: Cell Culture

    Synergy in bactericidal action. Histogram showing results for untreated and antibiotic-treated samples of E. coli ML35p. The antibiotic concentrations used are indicated below the bars. Cells were treated with or without drug for 18 h and plated on MH agar plates. After 20 h of incubation at 37°C, the colonies were counted. Synergy in bactericidal activity was observed for Ud and rifampin at an FBC index of 0.16. The log 10 CFU/ml observed at the end point is indicated above each bar. Standard deviations from triplicate experiments are plotted. RIF, rifampin.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Synergy with Rifampin and Kanamycin Enhances Potency, Kill Kinetics, and Selectivity of DeNovo-Designed Antimicrobial Peptides ▿

    doi: 10.1128/AAC.01231-09

    Figure Lengend Snippet: Synergy in bactericidal action. Histogram showing results for untreated and antibiotic-treated samples of E. coli ML35p. The antibiotic concentrations used are indicated below the bars. Cells were treated with or without drug for 18 h and plated on MH agar plates. After 20 h of incubation at 37°C, the colonies were counted. Synergy in bactericidal activity was observed for Ud and rifampin at an FBC index of 0.16. The log 10 CFU/ml observed at the end point is indicated above each bar. Standard deviations from triplicate experiments are plotted. RIF, rifampin.

    Article Snippet: ΔFq is a potent, broad-spectrum antibiotic, with action on both Gram-negative and Gram-positive bacterial membranes (MIC of 21.3 μg/ml [3.7 μM] against E. coli ML35p and 23 μg/ml [4 μM] against Staphylococcus aureus ATCC 23219).

    Techniques: Incubation, Activity Assay

    Synergy enhances kill kinetics of antimicrobial peptides. Kill kinetics of ΔFmscr and the combination of ΔFmscr and kanamycin (KAN) at an FIC index of 0.5 against E. coli ML35p at the concentrations indicated.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Synergy with Rifampin and Kanamycin Enhances Potency, Kill Kinetics, and Selectivity of DeNovo-Designed Antimicrobial Peptides ▿

    doi: 10.1128/AAC.01231-09

    Figure Lengend Snippet: Synergy enhances kill kinetics of antimicrobial peptides. Kill kinetics of ΔFmscr and the combination of ΔFmscr and kanamycin (KAN) at an FIC index of 0.5 against E. coli ML35p at the concentrations indicated.

    Article Snippet: ΔFq is a potent, broad-spectrum antibiotic, with action on both Gram-negative and Gram-positive bacterial membranes (MIC of 21.3 μg/ml [3.7 μM] against E. coli ML35p and 23 μg/ml [4 μM] against Staphylococcus aureus ATCC 23219).

    Techniques:

    5-Iodoindole does not induce E. coli and S. aureus persister cell formation. Exponential-phase cultures of E. coli K-12 BW25113 ( a ) or S. aureus (MSSA) 6538 ( b ) were exposed to indole (2 mM), rifampicin (100 μg mL −1 ), ampicillin (100 μg mL −1 ), or 5-iodoindole (2 mM) at 37 °C, 250 rpm. Cell viabilities were then determined. The experiment was performed in triplicate

    Journal: AMB Express

    Article Title: Halogenated indoles eradicate bacterial persister cells and biofilms

    doi: 10.1186/s13568-016-0297-6

    Figure Lengend Snippet: 5-Iodoindole does not induce E. coli and S. aureus persister cell formation. Exponential-phase cultures of E. coli K-12 BW25113 ( a ) or S. aureus (MSSA) 6538 ( b ) were exposed to indole (2 mM), rifampicin (100 μg mL −1 ), ampicillin (100 μg mL −1 ), or 5-iodoindole (2 mM) at 37 °C, 250 rpm. Cell viabilities were then determined. The experiment was performed in triplicate

    Article Snippet: Briefly, E. coli BW25113 was inoculated into 25 mL of LB medium in 250 mL shake flasks at a starting turbidity of 0.005 at 600 nm and grown to a turbidity of 0.8.

    Techniques:

    5-Iodoindole does not affect cell morphology. Exponential-phase cultures of E. coli K-12 BW25113 ( a ) or S. aureus (MSSA) 6538 ( b ) were exposure to rifampicin (100 μg mL −1 ) for 30 min and then incubated with 5-iodoindole (2 mM) or DMSO for 1 h then visualized by transmission electron microscopy. The scale bars represent 500 nm

    Journal: AMB Express

    Article Title: Halogenated indoles eradicate bacterial persister cells and biofilms

    doi: 10.1186/s13568-016-0297-6

    Figure Lengend Snippet: 5-Iodoindole does not affect cell morphology. Exponential-phase cultures of E. coli K-12 BW25113 ( a ) or S. aureus (MSSA) 6538 ( b ) were exposure to rifampicin (100 μg mL −1 ) for 30 min and then incubated with 5-iodoindole (2 mM) or DMSO for 1 h then visualized by transmission electron microscopy. The scale bars represent 500 nm

    Article Snippet: Briefly, E. coli BW25113 was inoculated into 25 mL of LB medium in 250 mL shake flasks at a starting turbidity of 0.005 at 600 nm and grown to a turbidity of 0.8.

    Techniques: Incubation, Transmission Assay, Electron Microscopy

    Indole derivatives kill E. coli persister cells. Escherichia coli K-12 BW25113 cells were exposed to rifampicin (100 μg mL −1 ) to induce persister cells. Indole or indole derivatives at 2 mM were then administered for 3 h at 37 °C and 250 rpm, and cell viabilities were determined. All indole compounds were dissolved in DMSO, and DMSO was used as the control (none). For the most active compounds, 5-iodoindole and 4-fluoroindole, two concentrations (1 and 2 mM) were tested. The experiment was performed in duplicate

    Journal: AMB Express

    Article Title: Halogenated indoles eradicate bacterial persister cells and biofilms

    doi: 10.1186/s13568-016-0297-6

    Figure Lengend Snippet: Indole derivatives kill E. coli persister cells. Escherichia coli K-12 BW25113 cells were exposed to rifampicin (100 μg mL −1 ) to induce persister cells. Indole or indole derivatives at 2 mM were then administered for 3 h at 37 °C and 250 rpm, and cell viabilities were determined. All indole compounds were dissolved in DMSO, and DMSO was used as the control (none). For the most active compounds, 5-iodoindole and 4-fluoroindole, two concentrations (1 and 2 mM) were tested. The experiment was performed in duplicate

    Article Snippet: Briefly, E. coli BW25113 was inoculated into 25 mL of LB medium in 250 mL shake flasks at a starting turbidity of 0.005 at 600 nm and grown to a turbidity of 0.8.

    Techniques:

    5-Iodoindole eradicates E. coli persister cells. Escherichia coli K-12 BW25113 cells in the exponential growth stage ( a ) or stationary growth stage ( b ) were exposed to rifampicin (100 μg mL −1 ) to induce persister cells, which were then treated with 5-iodoindole for 3 h at 37 °C and 250 rpm. Cell viabilities were determined. Ampicillin at 100 μg mL −1 ( c ) or ciprofloxacin at 0.5 μg mL −1 ( d ) were used to induce persister cells and then 5-iodoindole was treated. The experiment was performed in duplicate. N/D represents eradication below the limit of detection

    Journal: AMB Express

    Article Title: Halogenated indoles eradicate bacterial persister cells and biofilms

    doi: 10.1186/s13568-016-0297-6

    Figure Lengend Snippet: 5-Iodoindole eradicates E. coli persister cells. Escherichia coli K-12 BW25113 cells in the exponential growth stage ( a ) or stationary growth stage ( b ) were exposed to rifampicin (100 μg mL −1 ) to induce persister cells, which were then treated with 5-iodoindole for 3 h at 37 °C and 250 rpm. Cell viabilities were determined. Ampicillin at 100 μg mL −1 ( c ) or ciprofloxacin at 0.5 μg mL −1 ( d ) were used to induce persister cells and then 5-iodoindole was treated. The experiment was performed in duplicate. N/D represents eradication below the limit of detection

    Article Snippet: Briefly, E. coli BW25113 was inoculated into 25 mL of LB medium in 250 mL shake flasks at a starting turbidity of 0.005 at 600 nm and grown to a turbidity of 0.8.

    Techniques:

    Substituted indoles reduce the biofilm formation of E. coli and S. aureus. Escherichia coli K-12 BW25113 ( a ), S. aureus (MSSA) 6538 ( b ), and P. aeruginosa ( c ) biofilm formation was quantified after culturing with indoles for 24 h in 96-well plates. Error bars indicate SDs. For confocal laser microscope analysis, biofilm formation by E. coli K-12 BW25113 ( d ) and S. aureus (MSSA) 6538 ( e ) was observed in 96-well plates in the presence and absence of 5-iodoindole. The scale bars represent 100 μm. The experiment was performed in triplicate

    Journal: AMB Express

    Article Title: Halogenated indoles eradicate bacterial persister cells and biofilms

    doi: 10.1186/s13568-016-0297-6

    Figure Lengend Snippet: Substituted indoles reduce the biofilm formation of E. coli and S. aureus. Escherichia coli K-12 BW25113 ( a ), S. aureus (MSSA) 6538 ( b ), and P. aeruginosa ( c ) biofilm formation was quantified after culturing with indoles for 24 h in 96-well plates. Error bars indicate SDs. For confocal laser microscope analysis, biofilm formation by E. coli K-12 BW25113 ( d ) and S. aureus (MSSA) 6538 ( e ) was observed in 96-well plates in the presence and absence of 5-iodoindole. The scale bars represent 100 μm. The experiment was performed in triplicate

    Article Snippet: Briefly, E. coli BW25113 was inoculated into 25 mL of LB medium in 250 mL shake flasks at a starting turbidity of 0.005 at 600 nm and grown to a turbidity of 0.8.

    Techniques: Microscopy

    Single correlation between cell killing and PC. A similar correlation is observed for the two bacterial species, including the resistant E. coli strains CB1000 and CB2000, and for both means of irradiation. Cell survival and PC are shown as the mean and

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Protein damage and death by radiation in Escherichia coli and Deinococcus radiodurans

    doi: 10.1073/pnas.1009312107

    Figure Lengend Snippet: Single correlation between cell killing and PC. A similar correlation is observed for the two bacterial species, including the resistant E. coli strains CB1000 and CB2000, and for both means of irradiation. Cell survival and PC are shown as the mean and

    Article Snippet: The following bacterial strains were used: E. coli MG1655 wild type, E. coli ΔrecA::kan, E. coli CB1000, E. coli CB2000, E. coli CB founder strain (MG1655), D. radiodurans R1 (ATCC 13939) wild type, and D. radiodurans ΔrecA::tet.

    Techniques: Irradiation

    Effect of heavy metal on antibiotic resistance pattern of E. coli MRC11. R (resistant), I (intermediate) and S (sensitive) category as per CLSI criteria were labelled on each column. WM represented sensitivity in absence of heavy metal; C1, C2, C3 and C4 were concentrations of heavy metals in the medium, that were, Cd (175, 75, 50, 5 mg/L), Cu (250, 100, 50, 5 mg/L), Cr (125, 30, 15, 5 mg/L), Hg (15, 7.5, 1, 0.1 mg/L), Pb (250, 125, 75, 10 mg/L) and Zn (500, 250, 100, 50 mg/L) respectively. NG showed no growth of E. coli MRC11 isolate at that particular concentration of heavy metal.

    Journal: Brazilian Journal of Microbiology

    Article Title: Study of pandrug and heavy metal resistance among E. coli from anthropogenically influenced Delhi stretch of river Yamuna

    doi: 10.1016/j.bjm.2017.11.001

    Figure Lengend Snippet: Effect of heavy metal on antibiotic resistance pattern of E. coli MRC11. R (resistant), I (intermediate) and S (sensitive) category as per CLSI criteria were labelled on each column. WM represented sensitivity in absence of heavy metal; C1, C2, C3 and C4 were concentrations of heavy metals in the medium, that were, Cd (175, 75, 50, 5 mg/L), Cu (250, 100, 50, 5 mg/L), Cr (125, 30, 15, 5 mg/L), Hg (15, 7.5, 1, 0.1 mg/L), Pb (250, 125, 75, 10 mg/L) and Zn (500, 250, 100, 50 mg/L) respectively. NG showed no growth of E. coli MRC11 isolate at that particular concentration of heavy metal.

    Article Snippet: Biofilm formation in nutrient media was around 59% in E. coli MRC11 and 79% in P. aeruginosa ATCC 9027 ( ).

    Techniques: Concentration Assay

    Growth pattern of bacteria under different culture conditions. (A) Isolate E. coli MRC11, (B) isolate E. coli J53 trans-conjugant, (C) positive control (i.e., isolate resistant to β-lactams and heavy metals) and (D) sensitive control E. coli ATCC 25922. The X axis depicts time in hours where as Y axis represents bacterial growth presented in terms of OD at 600 nm.

    Journal: Brazilian Journal of Microbiology

    Article Title: Study of pandrug and heavy metal resistance among E. coli from anthropogenically influenced Delhi stretch of river Yamuna

    doi: 10.1016/j.bjm.2017.11.001

    Figure Lengend Snippet: Growth pattern of bacteria under different culture conditions. (A) Isolate E. coli MRC11, (B) isolate E. coli J53 trans-conjugant, (C) positive control (i.e., isolate resistant to β-lactams and heavy metals) and (D) sensitive control E. coli ATCC 25922. The X axis depicts time in hours where as Y axis represents bacterial growth presented in terms of OD at 600 nm.

    Article Snippet: Biofilm formation in nutrient media was around 59% in E. coli MRC11 and 79% in P. aeruginosa ATCC 9027 ( ).

    Techniques: Positive Control

    Effect of nhlF on NHase activity of the R. rhodochrous ATCC 12674 transformants. Each transformant was grown for 24 h at 28°C in MYP medium containing CoCl 2 as indicated. The NHase activity was measured as described. Solid boxes, R. rhodochrous ATCC 12674/pLJK50; dotted boxes, R. rhodochrous ATCC 12674/pLJK60.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A novel transporter involved in cobalt uptake

    doi:

    Figure Lengend Snippet: Effect of nhlF on NHase activity of the R. rhodochrous ATCC 12674 transformants. Each transformant was grown for 24 h at 28°C in MYP medium containing CoCl 2 as indicated. The NHase activity was measured as described. Solid boxes, R. rhodochrous ATCC 12674/pLJK50; dotted boxes, R. rhodochrous ATCC 12674/pLJK60.

    Article Snippet: To test this possibility, nhlF modified in the sequence upstream from its presumptive start codon was introduced into E. coli JM109, and cobalt uptake activity in the resultant E. coli transformants was investigated by the same method as in the case of the R. rhodochrous ATCC 12674 transformants with the exception that E. coli transformants replaced Rhodococcus transformants.

    Techniques: Activity Assay

    Examination of cobalt uptake in the R. rhodochrous or E. coli transformants. ( A ) Cobalt uptake of the R. rhodochrous ATCC 12674 transformants. The reaction mixture consisted of 10 nM 57 CoCl 2 , 10 mM MgCl 2 , and the cells in a 50 mM Tris·HCl buffer (pH 7.5). ○, R. rhodochrous ATCC 12674/pLJK50; ⋄, R. rhodochrous ATCC 12674/pLJK60; □, R. rhodochrous ATCC 12674/pK4. ( B ) Effect of other transient metals on the cobalt uptake of the R. rhodochrous ATCC 12674/pLJK50. Each metal was added at the final concentration of 5 μM, 10 min before the addition of 57 CoCl 2 into the cell suspension. □, MnCl 2 ; ▵, FeSO 4 ; ○, NiCl 2 ; ⊞, CuSO 4 . ( C ) Cobalt uptake by the E. coli JM109 transformants. The reaction mixture consisted of 10 nM 57 CoCl 2 , 10 mM MgCl 2 and the cells in a 50 mM Tris-hydrochloride buffer (pH 7.5). □, E. coli JM109/pLCO10; ○, E. coli JM109/pLCO20; ◊, E. coli JM109/pUC19.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: A novel transporter involved in cobalt uptake

    doi:

    Figure Lengend Snippet: Examination of cobalt uptake in the R. rhodochrous or E. coli transformants. ( A ) Cobalt uptake of the R. rhodochrous ATCC 12674 transformants. The reaction mixture consisted of 10 nM 57 CoCl 2 , 10 mM MgCl 2 , and the cells in a 50 mM Tris·HCl buffer (pH 7.5). ○, R. rhodochrous ATCC 12674/pLJK50; ⋄, R. rhodochrous ATCC 12674/pLJK60; □, R. rhodochrous ATCC 12674/pK4. ( B ) Effect of other transient metals on the cobalt uptake of the R. rhodochrous ATCC 12674/pLJK50. Each metal was added at the final concentration of 5 μM, 10 min before the addition of 57 CoCl 2 into the cell suspension. □, MnCl 2 ; ▵, FeSO 4 ; ○, NiCl 2 ; ⊞, CuSO 4 . ( C ) Cobalt uptake by the E. coli JM109 transformants. The reaction mixture consisted of 10 nM 57 CoCl 2 , 10 mM MgCl 2 and the cells in a 50 mM Tris-hydrochloride buffer (pH 7.5). □, E. coli JM109/pLCO10; ○, E. coli JM109/pLCO20; ◊, E. coli JM109/pUC19.

    Article Snippet: To test this possibility, nhlF modified in the sequence upstream from its presumptive start codon was introduced into E. coli JM109, and cobalt uptake activity in the resultant E. coli transformants was investigated by the same method as in the case of the R. rhodochrous ATCC 12674 transformants with the exception that E. coli transformants replaced Rhodococcus transformants.

    Techniques: Concentration Assay

    (A) Plasmid map of p16S lux with relevant restriction sites and arrangement of the E. coli DH10B 16S sequence (blue), the P help promoter region (green arrow), and luxABCDE (red arrows). (B) Gram-negative strains tagged by chromosomal integration of p16S

    Journal: Applied and Environmental Microbiology

    Article Title: Construction of p16Slux, a Novel Vector for Improved Bioluminescent Labeling of Gram-Negative Bacteria ▿

    doi: 10.1128/AEM.01394-07

    Figure Lengend Snippet: (A) Plasmid map of p16S lux with relevant restriction sites and arrangement of the E. coli DH10B 16S sequence (blue), the P help promoter region (green arrow), and luxABCDE (red arrows). (B) Gram-negative strains tagged by chromosomal integration of p16S

    Article Snippet: By using this protocol, the following organisms were rendered bioluminescent: E. coli DH10B, C. rodentium ICC169, Salmonella enterica serovar Typhimurium UK-1, P. aeruginosa PAO1, Enterobacter sakazakii DPC6440, Shigella flexneri 2a ATCC 700930, and Y. enterocolitica NCTC13174 (Fig. ).

    Techniques: Plasmid Preparation, Sequencing

    Southern blot analysis of traC in E. coli and P. stewartii subsp. stewartii . (A) Five micrograms of DNA from E. coli DH5α (lane 1) and E. coli HB101 (lane 2) was digested with BglII/NotI. Lane 3 was loaded with P. stewartii subsp. stewartii SW2

    Journal: Journal of Bacteriology

    Article Title: Stabilization of pSW100 from Pantoea stewartii by the F Conjugation System

    doi: 10.1128/JB.00846-07

    Figure Lengend Snippet: Southern blot analysis of traC in E. coli and P. stewartii subsp. stewartii . (A) Five micrograms of DNA from E. coli DH5α (lane 1) and E. coli HB101 (lane 2) was digested with BglII/NotI. Lane 3 was loaded with P. stewartii subsp. stewartii SW2

    Article Snippet: However, pSW140K was extremely unstable in E. coli DH5α, E. coli ATCC 23744, and E. coli ATCC 23846; 95%, 94%, and 77% of the population, respectively, had lost the plasmid after 84 generations of culturing (Fig. ).

    Techniques: Southern Blot

    EC 50 of SeNPs and TeNPs for planktonic and biofilm cultures of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) after 4 h of exposure and 24 h of growth + 24 h of exposure .

    Journal: Frontiers in Microbiology

    Article Title: Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms

    doi: 10.3389/fmicb.2015.00584

    Figure Lengend Snippet: EC 50 of SeNPs and TeNPs for planktonic and biofilm cultures of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) after 4 h of exposure and 24 h of growth + 24 h of exposure .

    Article Snippet: Evaluation of antimicrobial and antibiofilm activity of nanoparticles The susceptibility of E. coli JM109, P. aeruginosa PAO1 and S. aureus ATCC 25923 biofilm and planktonic population to the different biogenic NPs was evaluated.

    Techniques:

    MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of SeNPs ( n = 3) .

    Journal: Frontiers in Microbiology

    Article Title: Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms

    doi: 10.3389/fmicb.2015.00584

    Figure Lengend Snippet: MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of SeNPs ( n = 3) .

    Article Snippet: Evaluation of antimicrobial and antibiofilm activity of nanoparticles The susceptibility of E. coli JM109, P. aeruginosa PAO1 and S. aureus ATCC 25923 biofilm and planktonic population to the different biogenic NPs was evaluated.

    Techniques:

    MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of TeNPs ( n = 3) .

    Journal: Frontiers in Microbiology

    Article Title: Biogenic selenium and tellurium nanoparticles synthesized by environmental microbial isolates efficaciously inhibit bacterial planktonic cultures and biofilms

    doi: 10.3389/fmicb.2015.00584

    Figure Lengend Snippet: MBEC assays performed on the planktonic and biofilm population of E. coli JM109 (A), P. aeruginosa PAO1 (B), and S. aureus ATCC 25923 (C) exposed to increasing concentrations of TeNPs ( n = 3) .

    Article Snippet: Evaluation of antimicrobial and antibiofilm activity of nanoparticles The susceptibility of E. coli JM109, P. aeruginosa PAO1 and S. aureus ATCC 25923 biofilm and planktonic population to the different biogenic NPs was evaluated.

    Techniques:

    Chemical structures of bulgecins (A) and monobactams (B) produced by ATCC 31363 and ATCC 31433. (C) Bulgecin extract and aztreonam display potentiation against E. coli MC1061 in liquid culture and (D) detection of bulgecin A in culture extract. (C) E. coli

    Journal: ACS Chemical Biology

    Article Title: Whole-Genome Shotgun Sequencing of Two β-Proteobacterial Species in Search of the Bulgecin Biosynthetic Cluster

    doi: 10.1021/acschembio.7b00687

    Figure Lengend Snippet: Chemical structures of bulgecins (A) and monobactams (B) produced by ATCC 31363 and ATCC 31433. (C) Bulgecin extract and aztreonam display potentiation against E. coli MC1061 in liquid culture and (D) detection of bulgecin A in culture extract. (C) E. coli

    Article Snippet: E. coli MC1061 and ATCC 31433 (isolate originating at ATCC) were generously provided by Professor Marion Skalweit.

    Techniques: Produced

    AI-2 removal profile for bacteria producing AI-2. Shown is extracellular AI-2 activity in cell-free culture fluids from LuxS + strains E. coli MG1655 (triangles) and UPEC UTI89 (circles) (A) and B. cereus (diamonds) and B. anthracis (squares) (B)

    Journal: Journal of Bacteriology

    Article Title: Identification of Functional LsrB-Like Autoinducer-2 Receptors ▿Identification of Functional LsrB-Like Autoinducer-2 Receptors ▿ †

    doi: 10.1128/JB.00976-09

    Figure Lengend Snippet: AI-2 removal profile for bacteria producing AI-2. Shown is extracellular AI-2 activity in cell-free culture fluids from LuxS + strains E. coli MG1655 (triangles) and UPEC UTI89 (circles) (A) and B. cereus (diamonds) and B. anthracis (squares) (B)

    Article Snippet: Like E. coli MG1655, two Bacillus strains, B. cereus (ATCC 1087) and B. anthracis (vaccine strain Sterne 34F2), have orthologs classified as belonging to group I. Putative AI-2 receptors were identified in these species previously ( , ) but not confirmed experimentally.

    Techniques: Activity Assay

    Analysis of IscU expression and purification. ( A ) SDS/PAGE of overproduction and purification. Lane 1, molecular weight standards; lane 2, BL-21 cells harboring pTrcIscU (25 μg of protein); lane 3, BL-21 cells harboring pTrcIscU plasmid grown for ≈16 h after IPTG induction (25 μg of protein); lanes 4–6, purified IscU. ( B ) Immunoblot analysis of constitutive IscU expression in W3110, DH5α, and BL-21 cells grown to stationary phase in Terrific Broth. Lanes 1–3, purified IscU; lanes 4–6, E. coli whole cells (15 μg of protein each).

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Interaction of the iron-sulfur cluster assembly protein IscU with the Hsc66/Hsc20 molecular chaperone system of Escherichiacoli

    doi:

    Figure Lengend Snippet: Analysis of IscU expression and purification. ( A ) SDS/PAGE of overproduction and purification. Lane 1, molecular weight standards; lane 2, BL-21 cells harboring pTrcIscU (25 μg of protein); lane 3, BL-21 cells harboring pTrcIscU plasmid grown for ≈16 h after IPTG induction (25 μg of protein); lanes 4–6, purified IscU. ( B ) Immunoblot analysis of constitutive IscU expression in W3110, DH5α, and BL-21 cells grown to stationary phase in Terrific Broth. Lanes 1–3, purified IscU; lanes 4–6, E. coli whole cells (15 μg of protein each).

    Article Snippet: E. coli W3110 was from American Type Culture Collection (ATCC no. 27325); DH5αF′IQ cells were from GIBCO/BRL; and BL-21 (DE3)pLysS cells were from Novagen.

    Techniques: Expressing, Purification, SDS Page, Molecular Weight, Plasmid Preparation