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  • 97
    ATCC ermb vector
    Characterization of the <t>PyrF</t> mut mutant by PCR analysis and growth curves. (A) Diagram showing the PCR analysis of PyrF mut . Primers P 15 and P 16 locate at the flanking regions of pyrF ; primers P 3 and P 4 locate within the <t>ermB</t> cassette. The numbers are the
    Ermb Vector, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Characterization of the PyrF mut mutant by PCR analysis and growth curves. (A) Diagram showing the PCR analysis of PyrF mut . Primers P 15 and P 16 locate at the flanking regions of pyrF ; primers P 3 and P 4 locate within the ermB cassette. The numbers are the

    Journal: Applied and Environmental Microbiology

    Article Title: pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola

    doi: 10.1128/AEM.03704-15

    Figure Lengend Snippet: Characterization of the PyrF mut mutant by PCR analysis and growth curves. (A) Diagram showing the PCR analysis of PyrF mut . Primers P 15 and P 16 locate at the flanking regions of pyrF ; primers P 3 and P 4 locate within the ermB cassette. The numbers are the

    Article Snippet: To delete the pyrF gene ( tde2110 ) in frame, the pyrF :: ermB vector was constructed , linearized, and transformed into T. denticola strain ATCC 35405 (wild type), as described in Materials and Methods.

    Techniques: Mutagenesis, Polymerase Chain Reaction

    Diagrams illustrating the vectors of pyrF :: ermB and pFlgE in . (A) pyrF :: ermB was constructed by two-step PCR and used for in-frame replacement of the pyrF gene with the ermB cassette. (B) Plasmid pFlgE in was constructed to reintroduce the pyrF gene back

    Journal: Applied and Environmental Microbiology

    Article Title: pyrF as a Counterselectable Marker for Unmarked Genetic Manipulations in Treponema denticola

    doi: 10.1128/AEM.03704-15

    Figure Lengend Snippet: Diagrams illustrating the vectors of pyrF :: ermB and pFlgE in . (A) pyrF :: ermB was constructed by two-step PCR and used for in-frame replacement of the pyrF gene with the ermB cassette. (B) Plasmid pFlgE in was constructed to reintroduce the pyrF gene back

    Article Snippet: To delete the pyrF gene ( tde2110 ) in frame, the pyrF :: ermB vector was constructed , linearized, and transformed into T. denticola strain ATCC 35405 (wild type), as described in Materials and Methods.

    Techniques: Construct, Polymerase Chain Reaction, Plasmid Preparation