ermb vector Search Results


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  • 98
    Thermo Fisher gateway vector conversion kit
    Gateway Vector Conversion Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 86 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gateway vector conversion kit/product/Thermo Fisher
    Average 98 stars, based on 86 article reviews
    Price from $9.99 to $1999.99
    gateway vector conversion kit - by Bioz Stars, 2020-07
    98/100 stars
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    89
    Thermo Fisher vector prsetb
    Vector Prsetb, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector prsetb/product/Thermo Fisher
    Average 89 stars, based on 141 article reviews
    Price from $9.99 to $1999.99
    vector prsetb - by Bioz Stars, 2020-07
    89/100 stars
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    89
    Thermo Fisher pblunt vector
    Pblunt Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 75 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pblunt vector/product/Thermo Fisher
    Average 89 stars, based on 75 article reviews
    Price from $9.99 to $1999.99
    pblunt vector - by Bioz Stars, 2020-07
    89/100 stars
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    92
    InforMax Inc vector nti
    Vector Nti, supplied by InforMax Inc, used in various techniques. Bioz Stars score: 92/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector nti/product/InforMax Inc
    Average 92 stars, based on 102 article reviews
    Price from $9.99 to $1999.99
    vector nti - by Bioz Stars, 2020-07
    92/100 stars
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    91
    OriGene pcmv6 vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pcmv6 Vector, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 532 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcmv6 vector/product/OriGene
    Average 91 stars, based on 532 article reviews
    Price from $9.99 to $1999.99
    pcmv6 vector - by Bioz Stars, 2020-07
    91/100 stars
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    93
    Thermo Fisher pcr2 1 vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pcr2 1 Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 11220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr2 1 vector/product/Thermo Fisher
    Average 93 stars, based on 11220 article reviews
    Price from $9.99 to $1999.99
    pcr2 1 vector - by Bioz Stars, 2020-07
    93/100 stars
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    85
    Millipore plasmid vector pstblue 1
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Plasmid Vector Pstblue 1, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 35 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid vector pstblue 1/product/Millipore
    Average 85 stars, based on 35 article reviews
    Price from $9.99 to $1999.99
    plasmid vector pstblue 1 - by Bioz Stars, 2020-07
    85/100 stars
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    99
    TaKaRa pmd19 vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pmd19 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 154 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmd19 vector/product/TaKaRa
    Average 99 stars, based on 154 article reviews
    Price from $9.99 to $1999.99
    pmd19 vector - by Bioz Stars, 2020-07
    99/100 stars
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    99
    Promega pgem t vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pgem T Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 29265 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t vector/product/Promega
    Average 99 stars, based on 29265 article reviews
    Price from $9.99 to $1999.99
    pgem t vector - by Bioz Stars, 2020-07
    99/100 stars
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    88
    Thermo Fisher pcr 2 1 topo plasmid vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pcr 2 1 Topo Plasmid Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 247 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr 2 1 topo plasmid vector/product/Thermo Fisher
    Average 88 stars, based on 247 article reviews
    Price from $9.99 to $1999.99
    pcr 2 1 topo plasmid vector - by Bioz Stars, 2020-07
    88/100 stars
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    85
    TaKaRa vector pmd19t simple
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Vector Pmd19t Simple, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pmd19t simple/product/TaKaRa
    Average 85 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    vector pmd19t simple - by Bioz Stars, 2020-07
    85/100 stars
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    94
    Promega prl tk vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Prl Tk Vector, supplied by Promega, used in various techniques. Bioz Stars score: 94/100, based on 6721 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/prl tk vector/product/Promega
    Average 94 stars, based on 6721 article reviews
    Price from $9.99 to $1999.99
    prl tk vector - by Bioz Stars, 2020-07
    94/100 stars
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    92
    TaKaRa pegfp n2 vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pegfp N2 Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 994 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pegfp n2 vector/product/TaKaRa
    Average 92 stars, based on 994 article reviews
    Price from $9.99 to $1999.99
    pegfp n2 vector - by Bioz Stars, 2020-07
    92/100 stars
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    89
    Thermo Fisher pcr2 1topo vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pcr2 1topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr2 1topo vector/product/Thermo Fisher
    Average 89 stars, based on 351 article reviews
    Price from $9.99 to $1999.99
    pcr2 1topo vector - by Bioz Stars, 2020-07
    89/100 stars
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    99
    Qiagen pqe30 expression vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pqe30 Expression Vector, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1252 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pqe30 expression vector/product/Qiagen
    Average 99 stars, based on 1252 article reviews
    Price from $9.99 to $1999.99
    pqe30 expression vector - by Bioz Stars, 2020-07
    99/100 stars
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    92
    Thermo Fisher intermediate vector pjet1 2
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Intermediate Vector Pjet1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/intermediate vector pjet1 2/product/Thermo Fisher
    Average 92 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    intermediate vector pjet1 2 - by Bioz Stars, 2020-07
    92/100 stars
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    99
    Promega pgem t easy vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pgem T Easy Vector, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 92339 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pgem t easy vector/product/Promega
    Average 99 stars, based on 92339 article reviews
    Price from $9.99 to $1999.99
    pgem t easy vector - by Bioz Stars, 2020-07
    99/100 stars
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    94
    Thermo Fisher pcr blunt vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pcr Blunt Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2962 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr blunt vector/product/Thermo Fisher
    Average 94 stars, based on 2962 article reviews
    Price from $9.99 to $1999.99
    pcr blunt vector - by Bioz Stars, 2020-07
    94/100 stars
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    92
    Thermo Fisher vector pgem t easy
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Vector Pgem T Easy, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/vector pgem t easy/product/Thermo Fisher
    Average 92 stars, based on 41 article reviews
    Price from $9.99 to $1999.99
    vector pgem t easy - by Bioz Stars, 2020-07
    92/100 stars
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    85
    Promega pst blue 1 vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pst Blue 1 Vector, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pst blue 1 vector/product/Promega
    Average 85 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    pst blue 1 vector - by Bioz Stars, 2020-07
    85/100 stars
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    89
    Thermo Fisher coli vector pjet1 2
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Coli Vector Pjet1 2, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 90 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/coli vector pjet1 2/product/Thermo Fisher
    Average 89 stars, based on 90 article reviews
    Price from $9.99 to $1999.99
    coli vector pjet1 2 - by Bioz Stars, 2020-07
    89/100 stars
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    94
    Thermo Fisher pcrii topo vector
    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with <t>pCMV6</t> cDNA vector” value set as 1.
    Pcrii Topo Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 8012 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with pCMV6 cDNA vector” value set as 1.

    Journal: The Journal of Biological Chemistry

    Article Title: Regulation of c-Maf and αA-Crystallin in Ocular Lens by Fibroblast Growth Factor Signaling *

    doi: 10.1074/jbc.M115.705103

    Figure Lengend Snippet: Transcriptional regulation of c-Maf by c-Jun and Etv5/ERM. A , distribution of c-Jun and Etv5/ERM factors and H3 K4me3 promoter marker along the mouse c-Maf locus in lens chromatin. The locations of the qChIP amplicons covering 11 kb (−5 kb/+6 kb) c-Maf locus is shown at top . c-Jun, Etv5/ERM, and H3K4me3 are presented in the middle and lower panel , respectively. Statistically significant enrichment of binding signals is indicated above the horizontal dotted line . The relative enrichments are shown as 1% of the input. B , diagram of firefly luciferase reporter constructs without promoter (pGL3-Basic), with the intact −494/+210 c-Maf promoter (WT), with the c-Maf promoter lacking the −272/-70 FRE region (ΔFRE), and with three copies of the FRE followed by a minimal E4TATA promoter (3XFRE). C , results of transient co-transfection/reporter assays. The firefly luciferase activities were normalized using Renilla luciferase as an internal control. The results shown are from two independent experiments with duplicates. The relative luciferase activities were calculated using the “WT reporter with pCMV6 cDNA vector” value set as 1.

    Article Snippet: Transient co-transfections were conducted in a breast adenocarcinoma MCF-7 line previously used to study FGF signaling ( ) and αTN4-1 mouse lens epithelial cells ( ). c-Jun and Etv5 cDNAs in a pCMV6 vector were obtained from OriGene Technologies (Rockville, MD).

    Techniques: Marker, Binding Assay, Luciferase, Construct, Cotransfection, Plasmid Preparation