erlenmeyer flasks Search Results


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  • 99
    Thermo Fisher erlenmeyer flasks
    Erlenmeyer Flasks, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 366 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/Thermo Fisher
    Average 99 stars, based on 366 article reviews
    Price from $9.99 to $1999.99
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    96
    Millipore erlenmeyer flasks
    Changes in cell numbers (●), antifungal activity (■), and pH (○) over time. <t>Erlenmeyer</t> flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 10 5 L. coryniformis subsp. coryniformis Si3 per ml and incubated at 30°C on a rotary shaker.
    Erlenmeyer Flasks, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/Millipore
    Average 96 stars, based on 282 article reviews
    Price from $9.99 to $1999.99
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    92
    Corning Life Sciences erlenmeyer flasks
    Kinetics of substrate consumption and metabolite production in <t>Erlenmeyer</t> flask ( a , b and c ) and bioreactor ( A , B and C ): a , A glucose consumption ( open symbols ) and lactate production ( closed symbols ); b , B correlation between produced lactate and consumed
    Erlenmeyer Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 92/100, based on 294 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/Corning Life Sciences
    Average 92 stars, based on 294 article reviews
    Price from $9.99 to $1999.99
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    92/100 stars
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    95
    Millipore erlenmeyer flask
    Changes in cell numbers (●), antifungal activity (■), and pH (○) over time. <t>Erlenmeyer</t> flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 10 5 L. coryniformis subsp. coryniformis Si3 per ml and incubated at 30°C on a rotary shaker.
    Erlenmeyer Flask, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 185 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flask/product/Millipore
    Average 95 stars, based on 185 article reviews
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    erlenmeyer flask - by Bioz Stars, 2020-09
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    93
    Difco erlenmeyer flasks
    Cultures of Rhopalostroma angolense in 500 mL <t>Erlenmeyer</t> flasks supplied with 100 mL of Difco OA after 5 wk (a, b) and YMG medium after 6 wk (c), respectively, showing stromatal primordia (c); d, e. culture of R. angolense on a Difco OA plate (9 cm diam); e showing augmented section of d, revealing stromatal primordia and oily droplets in the centre of the colony. — Scale bars: a, b = 10 μm; c = 1 cm.
    Erlenmeyer Flasks, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 149 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 149 article reviews
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    92
    New Brunswick Scientific erlenmeyer flasks
    Cultures of Rhopalostroma angolense in 500 mL <t>Erlenmeyer</t> flasks supplied with 100 mL of Difco OA after 5 wk (a, b) and YMG medium after 6 wk (c), respectively, showing stromatal primordia (c); d, e. culture of R. angolense on a Difco OA plate (9 cm diam); e showing augmented section of d, revealing stromatal primordia and oily droplets in the centre of the colony. — Scale bars: a, b = 10 μm; c = 1 cm.
    Erlenmeyer Flasks, supplied by New Brunswick Scientific, used in various techniques. Bioz Stars score: 92/100, based on 110 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/New Brunswick Scientific
    Average 92 stars, based on 110 article reviews
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    89
    Corning Life Sciences polycarbonate erlenmeyer flasks
    Growth of different P. putida strains at 30°C and 180 rpm shaking with M9 medium in polycarbonate <t>Erlenmeyer</t> flasks supplemented with 5 mM 2-phenylethanol and 10 µM La 3+ in the absence (A) and presence of kanamycin (B and C) for plasmid maintenance. Flasks were inoculated at an OD 600 of 0.01 (A) or 0.03 (B and C) with washed cells from M9 overnight cultures grown with succinate in the absence (A) or presence (B and C) of kanamycin and 0.2% (wt/vol) rhamnose to induce pJEM[PedR2] and pJEM[PedR2 D53A ] plasmids. (A) Growth of Δ pedE (black circles), Δ pedE _PedS2 S178P (orange circles), and Δ pedE _PedS2 S178P Δ pedR2 (gray circles) strains. (B) Growth of Δ pedH _PedS2 S178P Δ pedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2 D53A ] (orange circles). (C) Growth of Δ pedE _PedS2 S178P Δ pedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2 D53A ] (orange circles). Data points represent the means for biological triplets, and error bars correspond to the respective standard deviations (positive error values).
    Polycarbonate Erlenmeyer Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 89/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polycarbonate erlenmeyer flasks/product/Corning Life Sciences
    Average 89 stars, based on 129 article reviews
    Price from $9.99 to $1999.99
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    93
    Avantor erlenmeyer flasks
    Protocol Scale Up to Rotated <t>Erlenmeyer</t> Flasks (A) Culture scheme in flasks rotated at 75 rpm. (B) Microscopic assessment of NKX2.5-GFP expression in EBs on day 13 of differentiation in response to 7.5 μM CHIR. (C) Immunofluorescent staining specific to α-actinin and cTNT on EB sections (top) and dissociated/plated cells derived thereof (bottom). (D) Flow cytometry on day 7–10 revealed ∼55% of GFP + (n = 5 of four independent experiments; mean ± SEM). (E) Representative flow cytometry histograms of day 10 EB-derived cells. See also Figure S1 .
    Erlenmeyer Flasks, supplied by Avantor, used in various techniques. Bioz Stars score: 93/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    SCHOTT erlenmeyer flasks
    Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, <t>Erlenmeyer</t> flasks; dashed lines, baffled flasks
    Erlenmeyer Flasks, supplied by SCHOTT, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    erlenmeyer flasks - by Bioz Stars, 2020-09
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    92
    Becton Dickinson erlenmeyer flasks
    Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, <t>Erlenmeyer</t> flasks; dashed lines, baffled flasks
    Erlenmeyer Flasks, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 92/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/Becton Dickinson
    Average 92 stars, based on 58 article reviews
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    90
    Corning Life Sciences disposable polycarbonate erlenmeyer flasks
    Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, <t>Erlenmeyer</t> flasks; dashed lines, baffled flasks
    Disposable Polycarbonate Erlenmeyer Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disposable polycarbonate erlenmeyer flasks/product/Corning Life Sciences
    Average 90 stars, based on 8 article reviews
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    92
    Merck & Co erlenmeyer flasks
    Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, <t>Erlenmeyer</t> flasks; dashed lines, baffled flasks
    Erlenmeyer Flasks, supplied by Merck & Co, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 26 article reviews
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    erlenmeyer flasks - by Bioz Stars, 2020-09
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    90
    Corning Life Sciences erlenmeyer shake flasks
    Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, <t>Erlenmeyer</t> flasks; dashed lines, baffled flasks
    Erlenmeyer Shake Flasks, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer shake flasks/product/Corning Life Sciences
    Average 90 stars, based on 39 article reviews
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    95
    Eppendorf AG erlenmeyer flask
    Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, <t>Erlenmeyer</t> flasks; dashed lines, baffled flasks
    Erlenmeyer Flask, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 95/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Fisher Scientific erlenmeyer flasks
    Effect of air-liquid interfacial area on enzymatic hydrolysis of Avicel with and without surfactant. Columns show cellulose conversion after 5, 11, and 17 days of enzymatic hydrolysis of Avicel (1% glucan loading) with 5 mg enzyme (Accellerase ® 1500) and with co-addition of 5 mg of Tween 20 added based on per gram glucan in substrate. Reaction volume was kept constant at 10 ml, and reactions were performed in 25, 125, and 500 ml <t>Erlenmeyer</t> flasks. Error bars represent standard deviation from three replicate flasks.
    Erlenmeyer Flasks, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 93/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/Fisher Scientific
    Average 93 stars, based on 38 article reviews
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    92
    SYGNIS AG erlenmeyer flasks
    Changes to the growth conditions break the circuit by changing host gene expression Doubling times for cells carrying the circuit grown in culture tubes (gray) and <t>Erlenmeyer</t> flasks (white). Error bars are calculated as the standard deviation from three biological replicates performed on different days. Comparison of host gene expression under culture tube conditions for sets of input states with differing numbers of expressed circuit genes (including yfp ): two = −/−/−, three = +/−/−, and four = −/+/−, +/+/−, −/−/+, +/−/+, −/+/+ and +/+/+ (0.5 mM IPTG/22 nM aTc/5 mM arabinose). The baseline is calculated from RNA‐seq data collected from cells harboring an empty circuit plasmid backbone (pAN1201). Points show the mean expression level of each gene. Red points denote genes with statistically significantly differential expression in comparison with the baseline ( P
    Erlenmeyer Flasks, supplied by SYGNIS AG, used in various techniques. Bioz Stars score: 92/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/SYGNIS AG
    Average 92 stars, based on 16 article reviews
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    erlenmeyer flasks - by Bioz Stars, 2020-09
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    92
    Bellco Glass erlenmeyer flasks
    Changes to the growth conditions break the circuit by changing host gene expression Doubling times for cells carrying the circuit grown in culture tubes (gray) and <t>Erlenmeyer</t> flasks (white). Error bars are calculated as the standard deviation from three biological replicates performed on different days. Comparison of host gene expression under culture tube conditions for sets of input states with differing numbers of expressed circuit genes (including yfp ): two = −/−/−, three = +/−/−, and four = −/+/−, +/+/−, −/−/+, +/−/+, −/+/+ and +/+/+ (0.5 mM IPTG/22 nM aTc/5 mM arabinose). The baseline is calculated from RNA‐seq data collected from cells harboring an empty circuit plasmid backbone (pAN1201). Points show the mean expression level of each gene. Red points denote genes with statistically significantly differential expression in comparison with the baseline ( P
    Erlenmeyer Flasks, supplied by Bellco Glass, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    erlenmeyer flasks - by Bioz Stars, 2020-09
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    86
    Corning Life Sciences polycarbonate erlenmeyer flask
    Changes to the growth conditions break the circuit by changing host gene expression Doubling times for cells carrying the circuit grown in culture tubes (gray) and <t>Erlenmeyer</t> flasks (white). Error bars are calculated as the standard deviation from three biological replicates performed on different days. Comparison of host gene expression under culture tube conditions for sets of input states with differing numbers of expressed circuit genes (including yfp ): two = −/−/−, three = +/−/−, and four = −/+/−, +/+/−, −/−/+, +/−/+, −/+/+ and +/+/+ (0.5 mM IPTG/22 nM aTc/5 mM arabinose). The baseline is calculated from RNA‐seq data collected from cells harboring an empty circuit plasmid backbone (pAN1201). Points show the mean expression level of each gene. Red points denote genes with statistically significantly differential expression in comparison with the baseline ( P
    Polycarbonate Erlenmeyer Flask, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 86/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polycarbonate erlenmeyer flask/product/Corning Life Sciences
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    93
    DSMZ erlenmeyer flasks
    Changes to the growth conditions break the circuit by changing host gene expression Doubling times for cells carrying the circuit grown in culture tubes (gray) and <t>Erlenmeyer</t> flasks (white). Error bars are calculated as the standard deviation from three biological replicates performed on different days. Comparison of host gene expression under culture tube conditions for sets of input states with differing numbers of expressed circuit genes (including yfp ): two = −/−/−, three = +/−/−, and four = −/+/−, +/+/−, −/−/+, +/−/+, −/+/+ and +/+/+ (0.5 mM IPTG/22 nM aTc/5 mM arabinose). The baseline is calculated from RNA‐seq data collected from cells harboring an empty circuit plasmid backbone (pAN1201). Points show the mean expression level of each gene. Red points denote genes with statistically significantly differential expression in comparison with the baseline ( P
    Erlenmeyer Flasks, supplied by DSMZ, used in various techniques. Bioz Stars score: 93/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erlenmeyer flasks/product/DSMZ
    Average 93 stars, based on 37 article reviews
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    erlenmeyer flasks - by Bioz Stars, 2020-09
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    Changes in cell numbers (●), antifungal activity (■), and pH (○) over time. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 10 5 L. coryniformis subsp. coryniformis Si3 per ml and incubated at 30°C on a rotary shaker.

    Journal: Applied and Environmental Microbiology

    Article Title: Lactobacillus coryniformis subsp. coryniformis Strain Si3 Produces a Broad-Spectrum Proteinaceous Antifungal Compound

    doi: 10.1128/AEM.67.1.1-5.2001

    Figure Lengend Snippet: Changes in cell numbers (●), antifungal activity (■), and pH (○) over time. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 10 5 L. coryniformis subsp. coryniformis Si3 per ml and incubated at 30°C on a rotary shaker.

    Article Snippet: L. coryniformis subsp. coryniformis Si3 was inoculated to a concentration of 105 cells/ml of 800 ml of MRS broth in 1,000-ml Erlenmeyer flasks, plugged with cotton to allow air access, and incubated as a still culture at 30°C for 48 h. The culture was then centrifuged (15,000 × g , 10 min), followed by filter sterilization (0.45-μm pore size; Millipore).

    Techniques: Activity Assay, Incubation

    Effect of gradual addition of ethanol on antifungal activity of L. coryniformis subsp. coryniformis Si3. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 5 × 10 5 cells per ml and incubated as still cultures at 30°C. Ethanol was added to reach a theoretical concentration of 2 mg/ml at 7 h, 3 mg/ml at 12 h, and 5 mg/ml at 15 h and a final concentration of 7 mg/ml at the early stationary phase. Cell numbers (●), antifungal activity (■), and pH (○) results are shown.

    Journal: Applied and Environmental Microbiology

    Article Title: Lactobacillus coryniformis subsp. coryniformis Strain Si3 Produces a Broad-Spectrum Proteinaceous Antifungal Compound

    doi: 10.1128/AEM.67.1.1-5.2001

    Figure Lengend Snippet: Effect of gradual addition of ethanol on antifungal activity of L. coryniformis subsp. coryniformis Si3. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 5 × 10 5 cells per ml and incubated as still cultures at 30°C. Ethanol was added to reach a theoretical concentration of 2 mg/ml at 7 h, 3 mg/ml at 12 h, and 5 mg/ml at 15 h and a final concentration of 7 mg/ml at the early stationary phase. Cell numbers (●), antifungal activity (■), and pH (○) results are shown.

    Article Snippet: L. coryniformis subsp. coryniformis Si3 was inoculated to a concentration of 105 cells/ml of 800 ml of MRS broth in 1,000-ml Erlenmeyer flasks, plugged with cotton to allow air access, and incubated as a still culture at 30°C for 48 h. The culture was then centrifuged (15,000 × g , 10 min), followed by filter sterilization (0.45-μm pore size; Millipore).

    Techniques: Activity Assay, Incubation, Concentration Assay

    Kinetics of substrate consumption and metabolite production in Erlenmeyer flask ( a , b and c ) and bioreactor ( A , B and C ): a , A glucose consumption ( open symbols ) and lactate production ( closed symbols ); b , B correlation between produced lactate and consumed

    Journal: Cytotechnology

    Article Title: Combination of yeast hydrolysates to improve CHO cell growth and IgG production

    doi: 10.1007/s10616-012-9519-1

    Figure Lengend Snippet: Kinetics of substrate consumption and metabolite production in Erlenmeyer flask ( a , b and c ) and bioreactor ( A , B and C ): a , A glucose consumption ( open symbols ) and lactate production ( closed symbols ); b , B correlation between produced lactate and consumed

    Article Snippet: CHO-AMW cells were routinely cultivated at 37 °C in 125 mL Erlenmeyer flasks (Corning, Amsterdam, The Netherlands) with 25 mL working volume and orbitally shaken at a frequency of 110 rpm.

    Techniques: Produced

    Kinetics of substrate consumption and metabolite production in Erlenmeyer flask ( a , b ) and bioreactor ( A , B ): a , A glutamine consumption ( open symbols ) and ammonia production ( closed symbols ); b , B Correlation between produced cells and consumed glutamine.

    Journal: Cytotechnology

    Article Title: Combination of yeast hydrolysates to improve CHO cell growth and IgG production

    doi: 10.1007/s10616-012-9519-1

    Figure Lengend Snippet: Kinetics of substrate consumption and metabolite production in Erlenmeyer flask ( a , b ) and bioreactor ( A , B ): a , A glutamine consumption ( open symbols ) and ammonia production ( closed symbols ); b , B Correlation between produced cells and consumed glutamine.

    Article Snippet: CHO-AMW cells were routinely cultivated at 37 °C in 125 mL Erlenmeyer flasks (Corning, Amsterdam, The Netherlands) with 25 mL working volume and orbitally shaken at a frequency of 110 rpm.

    Techniques: Produced

    Maximal cell concentration ( A ) and IgG production ( B ) of CHO-AMW cells cultivated in Erlenmeyer flask: without supplementation ( horizontal lines filled bar ), with supplementation of 1 g L −1 ( open bar ) or 4 g L −1 ( filled bar ) of YE, YP.A

    Journal: Cytotechnology

    Article Title: Combination of yeast hydrolysates to improve CHO cell growth and IgG production

    doi: 10.1007/s10616-012-9519-1

    Figure Lengend Snippet: Maximal cell concentration ( A ) and IgG production ( B ) of CHO-AMW cells cultivated in Erlenmeyer flask: without supplementation ( horizontal lines filled bar ), with supplementation of 1 g L −1 ( open bar ) or 4 g L −1 ( filled bar ) of YE, YP.A

    Article Snippet: CHO-AMW cells were routinely cultivated at 37 °C in 125 mL Erlenmeyer flasks (Corning, Amsterdam, The Netherlands) with 25 mL working volume and orbitally shaken at a frequency of 110 rpm.

    Techniques: Concentration Assay

    Kinetics of CHO cells cultivated in Erlenmeyer flask ( a , b ) and bioreactor ( A , B ): a , A viable cells ( open symbols ) and late apoptotic cells ( closed symbols ); b , B early apoptotic cells, performed without supplementation ( open circle ), with 1 g

    Journal: Cytotechnology

    Article Title: Combination of yeast hydrolysates to improve CHO cell growth and IgG production

    doi: 10.1007/s10616-012-9519-1

    Figure Lengend Snippet: Kinetics of CHO cells cultivated in Erlenmeyer flask ( a , b ) and bioreactor ( A , B ): a , A viable cells ( open symbols ) and late apoptotic cells ( closed symbols ); b , B early apoptotic cells, performed without supplementation ( open circle ), with 1 g

    Article Snippet: CHO-AMW cells were routinely cultivated at 37 °C in 125 mL Erlenmeyer flasks (Corning, Amsterdam, The Netherlands) with 25 mL working volume and orbitally shaken at a frequency of 110 rpm.

    Techniques:

    Kinetics of CHO cells cultivated in Erlenmeyer flask ( a – c ) and in bioreactor ( A – C ): a , A viable cells; ( b , B ) Trypan blue dead cells ( open symbols ) and lysed cells ( closed symbols ); c , C IgG production, performed without supplementation

    Journal: Cytotechnology

    Article Title: Combination of yeast hydrolysates to improve CHO cell growth and IgG production

    doi: 10.1007/s10616-012-9519-1

    Figure Lengend Snippet: Kinetics of CHO cells cultivated in Erlenmeyer flask ( a – c ) and in bioreactor ( A – C ): a , A viable cells; ( b , B ) Trypan blue dead cells ( open symbols ) and lysed cells ( closed symbols ); c , C IgG production, performed without supplementation

    Article Snippet: CHO-AMW cells were routinely cultivated at 37 °C in 125 mL Erlenmeyer flasks (Corning, Amsterdam, The Netherlands) with 25 mL working volume and orbitally shaken at a frequency of 110 rpm.

    Techniques:

    Transfection of small suspension cultures with Dicer-expressing plasmid. 293-6E cells were transfected with PEI:pTT5-DNA complexes at a 2:1 mass ratio and monitored after transfection. ( a ) Transfection efficiency at 48 hpt. A total of 36 ± 7% of the cells are expressing GFP ( n = 4). ( b ) Cell density and viability ( n = 4). ( c ) Expression of cytoplasmic Dicer. Dicer quantification was derived from Western blot analysis using a standard curve of pure recombinant Dicer ( n = 4). Transfections in ( b ) and ( c ) were performed in 20-mL cultures grown in 125-mL Erlenmeyer flasks and the results are means ± standard deviation values of replicates (n) from independent transfections experiments

    Journal: BMC Biotechnology

    Article Title: High-yield production of human Dicer by transfection of human HEK293-EBNA1 cells grown in suspension

    doi: 10.1186/s12896-018-0485-3

    Figure Lengend Snippet: Transfection of small suspension cultures with Dicer-expressing plasmid. 293-6E cells were transfected with PEI:pTT5-DNA complexes at a 2:1 mass ratio and monitored after transfection. ( a ) Transfection efficiency at 48 hpt. A total of 36 ± 7% of the cells are expressing GFP ( n = 4). ( b ) Cell density and viability ( n = 4). ( c ) Expression of cytoplasmic Dicer. Dicer quantification was derived from Western blot analysis using a standard curve of pure recombinant Dicer ( n = 4). Transfections in ( b ) and ( c ) were performed in 20-mL cultures grown in 125-mL Erlenmeyer flasks and the results are means ± standard deviation values of replicates (n) from independent transfections experiments

    Article Snippet: For optimization of transfection conditions, small-scale cultures were used: 2-mL cultures were grown in 6-well plates (Starstedt #83.1839.500) and 20-mL cultures were grown in 125-mL disposable Erlenmeyer flasks (Corning #431143).

    Techniques: Transfection, Expressing, Plasmid Preparation, Derivative Assay, Western Blot, Recombinant, Standard Deviation

    Changes in cell numbers (●), antifungal activity (■), and pH (○) over time. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 10 5 L. coryniformis subsp. coryniformis Si3 per ml and incubated at 30°C on a rotary shaker.

    Journal: Applied and Environmental Microbiology

    Article Title: Lactobacillus coryniformis subsp. coryniformis Strain Si3 Produces a Broad-Spectrum Proteinaceous Antifungal Compound

    doi: 10.1128/AEM.67.1.1-5.2001

    Figure Lengend Snippet: Changes in cell numbers (●), antifungal activity (■), and pH (○) over time. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 10 5 L. coryniformis subsp. coryniformis Si3 per ml and incubated at 30°C on a rotary shaker.

    Article Snippet: To obtain a larger amount of material, 800 ml of MRS broth in a cotton-plugged 1,000-ml Erlenmeyer flask was inoculated with 105 cells of L. coryniformis subsp. coryniformis Si3 per ml and grown as a still culture at 30°C for 48 h. After incubation the broth was centrifuged (15,000 × g , 10 min), sterile filtered (0.45-μm pore size; Millipore), and the filtrate was freeze-dried and adjusted to 15 times the original concentration in 20 mM citrate-phosphate buffer (pH 5.0).

    Techniques: Activity Assay, Incubation

    Effect of gradual addition of ethanol on antifungal activity of L. coryniformis subsp. coryniformis Si3. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 5 × 10 5 cells per ml and incubated as still cultures at 30°C. Ethanol was added to reach a theoretical concentration of 2 mg/ml at 7 h, 3 mg/ml at 12 h, and 5 mg/ml at 15 h and a final concentration of 7 mg/ml at the early stationary phase. Cell numbers (●), antifungal activity (■), and pH (○) results are shown.

    Journal: Applied and Environmental Microbiology

    Article Title: Lactobacillus coryniformis subsp. coryniformis Strain Si3 Produces a Broad-Spectrum Proteinaceous Antifungal Compound

    doi: 10.1128/AEM.67.1.1-5.2001

    Figure Lengend Snippet: Effect of gradual addition of ethanol on antifungal activity of L. coryniformis subsp. coryniformis Si3. Erlenmeyer flasks (250 ml), plugged with cotton (to allow air access), with 200 ml of MRS broth were inoculated with 5 × 10 5 cells per ml and incubated as still cultures at 30°C. Ethanol was added to reach a theoretical concentration of 2 mg/ml at 7 h, 3 mg/ml at 12 h, and 5 mg/ml at 15 h and a final concentration of 7 mg/ml at the early stationary phase. Cell numbers (●), antifungal activity (■), and pH (○) results are shown.

    Article Snippet: To obtain a larger amount of material, 800 ml of MRS broth in a cotton-plugged 1,000-ml Erlenmeyer flask was inoculated with 105 cells of L. coryniformis subsp. coryniformis Si3 per ml and grown as a still culture at 30°C for 48 h. After incubation the broth was centrifuged (15,000 × g , 10 min), sterile filtered (0.45-μm pore size; Millipore), and the filtrate was freeze-dried and adjusted to 15 times the original concentration in 20 mM citrate-phosphate buffer (pH 5.0).

    Techniques: Activity Assay, Incubation, Concentration Assay

    Cultures of Rhopalostroma angolense in 500 mL Erlenmeyer flasks supplied with 100 mL of Difco OA after 5 wk (a, b) and YMG medium after 6 wk (c), respectively, showing stromatal primordia (c); d, e. culture of R. angolense on a Difco OA plate (9 cm diam); e showing augmented section of d, revealing stromatal primordia and oily droplets in the centre of the colony. — Scale bars: a, b = 10 μm; c = 1 cm.

    Journal: Persoonia : Molecular Phylogeny and Evolution of Fungi

    Article Title: The phylogenetic position of Rhopalostroma as inferred from a polythetic approach

    doi: 10.3767/003158510X524231

    Figure Lengend Snippet: Cultures of Rhopalostroma angolense in 500 mL Erlenmeyer flasks supplied with 100 mL of Difco OA after 5 wk (a, b) and YMG medium after 6 wk (c), respectively, showing stromatal primordia (c); d, e. culture of R. angolense on a Difco OA plate (9 cm diam); e showing augmented section of d, revealing stromatal primordia and oily droplets in the centre of the colony. — Scale bars: a, b = 10 μm; c = 1 cm.

    Article Snippet: Cultures in 500 mL — Erlenmeyer flasks ( ) containing 20 g cellulose pulp or 100 mL Difco OA showed a similar morphology as those on agar plates, but produced semiglobose stromatal primordia to 2.5 cm diam after 4–6 wk.

    Techniques:

    Microscopic characteristics of Rhopalostroma angolense , from Difco OA plates (a, f, g) and 500 mL Erlenmeyer cultures, each after 5 wk of incubation. a. Thick-walled hyphae observed in melanising cultures; b–e. conidiophores, ranging from the simple Sporothrix to the more complex Virgariella types sensu Ju Rogers (1996) ; f–h. conidia; g. conidium arising laterally from a simple conidiogenous cell of the Sporothrix type sensu Ju Rogers (1996) . — Scale bars: a, d–h = 10 μm; b–e = 20 μm.

    Journal: Persoonia : Molecular Phylogeny and Evolution of Fungi

    Article Title: The phylogenetic position of Rhopalostroma as inferred from a polythetic approach

    doi: 10.3767/003158510X524231

    Figure Lengend Snippet: Microscopic characteristics of Rhopalostroma angolense , from Difco OA plates (a, f, g) and 500 mL Erlenmeyer cultures, each after 5 wk of incubation. a. Thick-walled hyphae observed in melanising cultures; b–e. conidiophores, ranging from the simple Sporothrix to the more complex Virgariella types sensu Ju Rogers (1996) ; f–h. conidia; g. conidium arising laterally from a simple conidiogenous cell of the Sporothrix type sensu Ju Rogers (1996) . — Scale bars: a, d–h = 10 μm; b–e = 20 μm.

    Article Snippet: Cultures in 500 mL — Erlenmeyer flasks ( ) containing 20 g cellulose pulp or 100 mL Difco OA showed a similar morphology as those on agar plates, but produced semiglobose stromatal primordia to 2.5 cm diam after 4–6 wk.

    Techniques: Incubation

    Growth of different P. putida strains at 30°C and 180 rpm shaking with M9 medium in polycarbonate Erlenmeyer flasks supplemented with 5 mM 2-phenylethanol and 10 µM La 3+ in the absence (A) and presence of kanamycin (B and C) for plasmid maintenance. Flasks were inoculated at an OD 600 of 0.01 (A) or 0.03 (B and C) with washed cells from M9 overnight cultures grown with succinate in the absence (A) or presence (B and C) of kanamycin and 0.2% (wt/vol) rhamnose to induce pJEM[PedR2] and pJEM[PedR2 D53A ] plasmids. (A) Growth of Δ pedE (black circles), Δ pedE _PedS2 S178P (orange circles), and Δ pedE _PedS2 S178P Δ pedR2 (gray circles) strains. (B) Growth of Δ pedH _PedS2 S178P Δ pedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2 D53A ] (orange circles). (C) Growth of Δ pedE _PedS2 S178P Δ pedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2 D53A ] (orange circles). Data points represent the means for biological triplets, and error bars correspond to the respective standard deviations (positive error values).

    Journal: mSphere

    Article Title: The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440

    doi: 10.1128/mSphere.00376-18

    Figure Lengend Snippet: Growth of different P. putida strains at 30°C and 180 rpm shaking with M9 medium in polycarbonate Erlenmeyer flasks supplemented with 5 mM 2-phenylethanol and 10 µM La 3+ in the absence (A) and presence of kanamycin (B and C) for plasmid maintenance. Flasks were inoculated at an OD 600 of 0.01 (A) or 0.03 (B and C) with washed cells from M9 overnight cultures grown with succinate in the absence (A) or presence (B and C) of kanamycin and 0.2% (wt/vol) rhamnose to induce pJEM[PedR2] and pJEM[PedR2 D53A ] plasmids. (A) Growth of Δ pedE (black circles), Δ pedE _PedS2 S178P (orange circles), and Δ pedE _PedS2 S178P Δ pedR2 (gray circles) strains. (B) Growth of Δ pedH _PedS2 S178P Δ pedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2 D53A ] (orange circles). (C) Growth of Δ pedE _PedS2 S178P Δ pedR2 strain harboring pJEM[PedR2] (gray circles) or pJEM[PedR2 D53A ] (orange circles). Data points represent the means for biological triplets, and error bars correspond to the respective standard deviations (positive error values).

    Article Snippet: All liquid growth experiments were carried out using modified M9 medium with 25 mM succinate or 5 mM 2-phenylethanol as the sole source of carbon and energy (see above) in 125-ml or 250-ml polycarbonate Erlenmeyer flasks (Corning) or in 96-well 2-ml deep-well plates (Carl Roth) as described previously ( ).

    Techniques: Plasmid Preparation

    (A to D) Schemes of selection (A), clonal isolation (B), characterization and single nucleotide polymorphism (SNP) identification (C and D) in the two-component sensor histidine kinase PedS2 of the Δ pedH S1 (R73C), Δ pedH S2 (R111W), Δ pedH S3 (S178P) spontaneous mutants. (A) Cells of the Δ pedH strain were incubated in M9 medium supplemented with 5 mM 2-phenylethanol and 10 µM LaCl 3 in plastic Erlenmeyer flasks ( n = 3) at 30°C with shaking at 180 rpm. (B) After growth was observed ( > 5 days), dilutions from each culture were plated onto LB agar plates and incubated at 30°C. Individual clones were further streaked on LB agar twice prior to further characterization. (C) Clones were characterized for their growth behavior in M9 medium with 5 mM 2-phenylethanol in the presence of 10 µM LaCl 3 . Subsequently, one clone from each culture exhibiting faster growth than the parental Δ pedH strain was used for PCR amplification of the pedS2 ). (D and E) Visualization of domain composition of PedS2 of P. putida (D) and MxaY of Methylomicrobium buryatense ).

    Journal: mSphere

    Article Title: The PedS2/PedR2 Two-Component System Is Crucial for the Rare Earth Element Switch in Pseudomonas putida KT2440

    doi: 10.1128/mSphere.00376-18

    Figure Lengend Snippet: (A to D) Schemes of selection (A), clonal isolation (B), characterization and single nucleotide polymorphism (SNP) identification (C and D) in the two-component sensor histidine kinase PedS2 of the Δ pedH S1 (R73C), Δ pedH S2 (R111W), Δ pedH S3 (S178P) spontaneous mutants. (A) Cells of the Δ pedH strain were incubated in M9 medium supplemented with 5 mM 2-phenylethanol and 10 µM LaCl 3 in plastic Erlenmeyer flasks ( n = 3) at 30°C with shaking at 180 rpm. (B) After growth was observed ( > 5 days), dilutions from each culture were plated onto LB agar plates and incubated at 30°C. Individual clones were further streaked on LB agar twice prior to further characterization. (C) Clones were characterized for their growth behavior in M9 medium with 5 mM 2-phenylethanol in the presence of 10 µM LaCl 3 . Subsequently, one clone from each culture exhibiting faster growth than the parental Δ pedH strain was used for PCR amplification of the pedS2 ). (D and E) Visualization of domain composition of PedS2 of P. putida (D) and MxaY of Methylomicrobium buryatense ).

    Article Snippet: All liquid growth experiments were carried out using modified M9 medium with 25 mM succinate or 5 mM 2-phenylethanol as the sole source of carbon and energy (see above) in 125-ml or 250-ml polycarbonate Erlenmeyer flasks (Corning) or in 96-well 2-ml deep-well plates (Carl Roth) as described previously ( ).

    Techniques: Selection, Isolation, Incubation, Clone Assay, Polymerase Chain Reaction, Amplification

    Protocol Scale Up to Rotated Erlenmeyer Flasks (A) Culture scheme in flasks rotated at 75 rpm. (B) Microscopic assessment of NKX2.5-GFP expression in EBs on day 13 of differentiation in response to 7.5 μM CHIR. (C) Immunofluorescent staining specific to α-actinin and cTNT on EB sections (top) and dissociated/plated cells derived thereof (bottom). (D) Flow cytometry on day 7–10 revealed ∼55% of GFP + (n = 5 of four independent experiments; mean ± SEM). (E) Representative flow cytometry histograms of day 10 EB-derived cells. See also Figure S1 .

    Journal: Stem Cell Reports

    Article Title: Controlling Expansion and Cardiomyogenic Differentiation of Human Pluripotent Stem Cells in Scalable Suspension Culture

    doi: 10.1016/j.stemcr.2014.09.017

    Figure Lengend Snippet: Protocol Scale Up to Rotated Erlenmeyer Flasks (A) Culture scheme in flasks rotated at 75 rpm. (B) Microscopic assessment of NKX2.5-GFP expression in EBs on day 13 of differentiation in response to 7.5 μM CHIR. (C) Immunofluorescent staining specific to α-actinin and cTNT on EB sections (top) and dissociated/plated cells derived thereof (bottom). (D) Flow cytometry on day 7–10 revealed ∼55% of GFP + (n = 5 of four independent experiments; mean ± SEM). (E) Representative flow cytometry histograms of day 10 EB-derived cells. See also Figure S1 .

    Article Snippet: For the inoculation of suspension culture, dissociation into single cells was performed by accutase treatment for 5 min at 37°C followed by dilution to 3.3 × 105 cells/ml in mTeSR1 plus 10 μM Y-27632 and seeding into 12-well suspension plates (GreinerBioOne) or Erlenmeyer flasks (125 ml scale; VWR-International) in 1.5 ml/well or 20 ml/flask, respectively.

    Techniques: Expressing, Staining, Derivative Assay, Flow Cytometry, Cytometry

    Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, Erlenmeyer flasks; dashed lines, baffled flasks

    Journal: Microbial Cell Factories

    Article Title: High-throughput optimization of medium components and culture conditions for the efficient production of a lipopeptide pseudofactin by Pseudomonas fluorescens BD5

    doi: 10.1186/s12934-018-0968-x

    Figure Lengend Snippet: Growth curves ( a ), PF production kinetics ( b ) and specific PF production kinetics ( c ) of P. fluorescens ” section. Solid lines, Erlenmeyer flasks; dashed lines, baffled flasks

    Article Snippet: Initial medium screening experiments were performed in 300-mL Erlenmeyer flasks (Schott DURAN), using 20% or 40% filling volumes.

    Techniques:

    Effect of air-liquid interfacial area on enzymatic hydrolysis of Avicel with and without surfactant. Columns show cellulose conversion after 5, 11, and 17 days of enzymatic hydrolysis of Avicel (1% glucan loading) with 5 mg enzyme (Accellerase ® 1500) and with co-addition of 5 mg of Tween 20 added based on per gram glucan in substrate. Reaction volume was kept constant at 10 ml, and reactions were performed in 25, 125, and 500 ml Erlenmeyer flasks. Error bars represent standard deviation from three replicate flasks.

    Journal: Scientific Reports

    Article Title: Deactivation of Cellulase at the Air-Liquid Interface Is the Main Cause of Incomplete Cellulose Conversion at Low Enzyme Loadings

    doi: 10.1038/s41598-018-19848-3

    Figure Lengend Snippet: Effect of air-liquid interfacial area on enzymatic hydrolysis of Avicel with and without surfactant. Columns show cellulose conversion after 5, 11, and 17 days of enzymatic hydrolysis of Avicel (1% glucan loading) with 5 mg enzyme (Accellerase ® 1500) and with co-addition of 5 mg of Tween 20 added based on per gram glucan in substrate. Reaction volume was kept constant at 10 ml, and reactions were performed in 25, 125, and 500 ml Erlenmeyer flasks. Error bars represent standard deviation from three replicate flasks.

    Article Snippet: Corning® Pyrex® 125 ml Erlenmeyer flasks made of borosilicate glass (Cat. No. 4985–125), 25 ml Erlenmeyer flasks made of borosilicate glass, Kimble® Kimax® Valueware® , (Cat. No. 5650025EMD), 125 ml Erlenmeyer flasks made of polycarbonate material Fisherbrand (Cat. No. PBV125), Corning® Pyrex® 500 ml Erlenmeyer flasks made of borosilicate glass (Cat. No. 4995-500), tetrahydrofuran (certified) and 72 w/w% sulfuric acid (Ricca Chemical) were all purchased through Fisher Scientific, Thermo Fisher Scientific Inc. at Waltham, MA.

    Techniques: Standard Deviation

    Changes to the growth conditions break the circuit by changing host gene expression Doubling times for cells carrying the circuit grown in culture tubes (gray) and Erlenmeyer flasks (white). Error bars are calculated as the standard deviation from three biological replicates performed on different days. Comparison of host gene expression under culture tube conditions for sets of input states with differing numbers of expressed circuit genes (including yfp ): two = −/−/−, three = +/−/−, and four = −/+/−, +/+/−, −/−/+, +/−/+, −/+/+ and +/+/+ (0.5 mM IPTG/22 nM aTc/5 mM arabinose). The baseline is calculated from RNA‐seq data collected from cells harboring an empty circuit plasmid backbone (pAN1201). Points show the mean expression level of each gene. Red points denote genes with statistically significantly differential expression in comparison with the baseline ( P

    Journal: Molecular Systems Biology

    Article Title: Genetic circuit characterization and debugging using RNA‐seq

    doi: 10.15252/msb.20167461

    Figure Lengend Snippet: Changes to the growth conditions break the circuit by changing host gene expression Doubling times for cells carrying the circuit grown in culture tubes (gray) and Erlenmeyer flasks (white). Error bars are calculated as the standard deviation from three biological replicates performed on different days. Comparison of host gene expression under culture tube conditions for sets of input states with differing numbers of expressed circuit genes (including yfp ): two = −/−/−, three = +/−/−, and four = −/+/−, +/+/−, −/−/+, +/−/+, −/+/+ and +/+/+ (0.5 mM IPTG/22 nM aTc/5 mM arabinose). The baseline is calculated from RNA‐seq data collected from cells harboring an empty circuit plasmid backbone (pAN1201). Points show the mean expression level of each gene. Red points denote genes with statistically significantly differential expression in comparison with the baseline ( P

    Article Snippet: Culture tubes and Erlenmeyer flasks were then grown in an Innova 44 shaker at 37°C and 250 rpm for five hours.

    Techniques: Expressing, Standard Deviation, RNA Sequencing Assay, Plasmid Preparation