Journal: Redox Biology
Article Title: α-Lipoic acid improves abnormal behavior by mitigation of oxidative stress, inflammation, ferroptosis, and tauopathy in P301S Tau transgenic mice
Figure Lengend Snippet: LA inhibited P38 MAPK, calpain1/CDK5 and GSK3β pathways but promoted PP2A. (A) Western blotting analysis of p-ERK1/2, ERK1/2, p-JNK1/2, JNK1/2, p-P38, and P38. β-actin served as the loading control. ( B-D) Quantitative analysis of p-ERK1/2, p-JNK1/2 and p-P38. ( E) Western blot detection of calpain1, p-CDK5, CDK5 and P35/25 in the brains. ( F-H) Quantification of calpain1, p-CDK5 and the P25/P35 ratio. (I) Western blot for p-GSK3α/β, GSK3α/β and PP2A expression. ( J-L) Quantitative analysis of p-GSK3α, p-GSK3β and PP2A. All results are presented as the mean ± SEM (n = 7). *p
Article Snippet: The primary antibodies used in the study were as follows: mouse-anti-apoptosis inducing factor (AIF, 1:1000, Santa Cruz), mouse-anti-Bcl2 (1:1000, Santa Cruz), rabbit-anti-Bax (1:1000, Santa Cruz), mouse-anti-Beclin1 (1:1000, ImmunoWay), rabbit-anti-calpain1 (1:2000, Abcam), rabbit-anti-p-cyclin dependent kinase 5 (Tyr15) (CDK5, 1:2000, Sigma), rabbit-anti-CDK5 (1:2000, Abcam), rabbit-anti-caspase3 (1:2000, CST), rabbit-anti-divalent mental transporter 1 (DMT1, 1:1000, Alpha diagnostic), rabbit-anti-p-extracellular regulated protein kinases1/2 (Thr202/Thr204) (ERK1/2, 1:2000, CST), rabbit-anti-ERK1/2 (1:2000, CST), goat-anti-ferroportin 1 (Fpn 1, 1:1000, Santa Cruz), rabbit-anti-glial fibrillary acidic protein (GFAP, 1:1000, Sigma), rabbit-anti-glutathione peroxidase 4 (GPx4, 1:1000, Santa Cruz), rabbit-anti-p-glycogen synthase kinase 3 alpha/βeta (Ser21/9) (p-GSK3α/β, 1:2000, CST), rabbit-anti-GSK3α/β (1:2000, CST), rabbit-anti-ionized binding adaptor protein 1 (Iba1, 1:1000, Abcam), rabbit-anti-interleukin 1 beta (IL-1β, 1:1000, Santa Cruz), mouse-anti-p-SAPK/JNK (Thr183/Tyr185) (1:2000, CST), rabbit-anti-SAPK/JNK (1:2000, CST), rabbit-anti-LC3A/B (1:2000, CST), rabbit-anti-NeuN (1:2000, CST), rabbit-anti-postsynaptic density protein 95 (PSD95, 1:2000, CST), rabbit-anti-p35/25 (1:2000, CST), rabbit-anti-protein phosphatase 2A C subunit (PP2A, 1:2000, CST), rabbit-anti-p-P38 mitogen-activated protein kinase (Thr180/Tyr182) (p-P38 MAPK, 1:2000, CST), rabbit-anti-P38 MAPK (1:2000, CST), rabbit-anti-superoxide dismutase 1 (SOD1, 1:1000, CST), mouse-anti-synaptophysin (SYP, 1:2000, Sigma), mouse-anti-tumor necrosis factor alpha (TNFα, 1:500, Santa Cruz), mouse-anti-transferrin receptor (TFR, 1:1000, Invitrogen), mouse-anti-Tau (Tau46) (1:2000, CST), rabbit-anti-p-Tau (Thr181) (1:2000, CST), rabbit-anti-p-Tau (Ser416) (1:2000, CST), rabbit-anti-p-Tau (Ser202) (1:2000, CST), rabbit-anti-p-Tau (Ser396) (1:1000, Sigma), rabbit-anti-p-Tau (Ser404) (1:2000, Sigma), rabbit-anti-p-Tau (Thr231) (1:1000, Sigma), rabbit-anti-cystine-glutamate antiporter (xCT, 1:2000, Abcam), and mouse-anti-β-actin (1:10000, Sigma).
Techniques: Western Blot, Expressing