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gene exp erc2 mm01209943 m1  (Thermo Fisher)
85
Thermo Fisher gene exp erc2 mm01209943 m1
Gene Exp Erc2 Mm01209943 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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erc2 approach  (Environment and Climate Change Canada)
86
Environment and Climate Change Canada erc2 approach
Erc2 Approach, supplied by Environment and Climate Change Canada, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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erc2  (Santa Cruz Biotechnology)
86
Santa Cruz Biotechnology erc2
Medium-scale immunoprecipitation of AMPA receptor complexes from the forebrain followed by peptide elution and LC-MS/MS analysis. A , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D1 (0.5% Triton X), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides is shown. This protein gel picture was a part of our previous publication . This picture was reused here to compare with other protein gel pictures and to report additional data shown in C. B , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D2 (0.5% Triton X + 0.1% SDS), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides. Legends and symbols are same as , A and B. C , shown is a summary of MS/MS analysis with a protein band Cx_6 that was excised from the protein gel in A. Most of peptide sequences were matched to the sequence of either <t>ERC2/CAST1</t> (#1, #2, #5, #6, and #7) or GEF-H1 (#9, #10, #11, and #12).
Erc2, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erc2/product/Santa Cruz Biotechnology
Average 86 stars, based on 1 article reviews
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erc2 - by Bioz Stars, 2024-10
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erc2 cast  (Synaptic Systems)
86
Synaptic Systems erc2 cast
Medium-scale immunoprecipitation of AMPA receptor complexes from the forebrain followed by peptide elution and LC-MS/MS analysis. A , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D1 (0.5% Triton X), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides is shown. This protein gel picture was a part of our previous publication . This picture was reused here to compare with other protein gel pictures and to report additional data shown in C. B , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D2 (0.5% Triton X + 0.1% SDS), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides. Legends and symbols are same as , A and B. C , shown is a summary of MS/MS analysis with a protein band Cx_6 that was excised from the protein gel in A. Most of peptide sequences were matched to the sequence of either <t>ERC2/CAST1</t> (#1, #2, #5, #6, and #7) or GEF-H1 (#9, #10, #11, and #12).
Erc2 Cast, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erc2 cast/product/Synaptic Systems
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erc2 cast - by Bioz Stars, 2024-10
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erc2  (Synaptic Systems)
86
Synaptic Systems erc2
Medium-scale immunoprecipitation of AMPA receptor complexes from the forebrain followed by peptide elution and LC-MS/MS analysis. A , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D1 (0.5% Triton X), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides is shown. This protein gel picture was a part of our previous publication . This picture was reused here to compare with other protein gel pictures and to report additional data shown in C. B , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D2 (0.5% Triton X + 0.1% SDS), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides. Legends and symbols are same as , A and B. C , shown is a summary of MS/MS analysis with a protein band Cx_6 that was excised from the protein gel in A. Most of peptide sequences were matched to the sequence of either <t>ERC2/CAST1</t> (#1, #2, #5, #6, and #7) or GEF-H1 (#9, #10, #11, and #12).
Erc2, supplied by Synaptic Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/erc2/product/Synaptic Systems
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
erc2 - by Bioz Stars, 2024-10
86/100 stars
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Image Search Results


Medium-scale immunoprecipitation of AMPA receptor complexes from the forebrain followed by peptide elution and LC-MS/MS analysis. A , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D1 (0.5% Triton X), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides is shown. This protein gel picture was a part of our previous publication . This picture was reused here to compare with other protein gel pictures and to report additional data shown in C. B , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D2 (0.5% Triton X + 0.1% SDS), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides. Legends and symbols are same as , A and B. C , shown is a summary of MS/MS analysis with a protein band Cx_6 that was excised from the protein gel in A. Most of peptide sequences were matched to the sequence of either ERC2/CAST1 (#1, #2, #5, #6, and #7) or GEF-H1 (#9, #10, #11, and #12).

Journal: The Journal of Biological Chemistry

Article Title: Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes

doi: 10.1074/jbc.M111.336644

Figure Lengend Snippet: Medium-scale immunoprecipitation of AMPA receptor complexes from the forebrain followed by peptide elution and LC-MS/MS analysis. A , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D1 (0.5% Triton X), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides is shown. This protein gel picture was a part of our previous publication . This picture was reused here to compare with other protein gel pictures and to report additional data shown in C. B , silver staining of a 4–12% gradient protein gel separating proteins that were solubilized with D2 (0.5% Triton X + 0.1% SDS), affinity-purified with WGA chromatography, co-immunoprecipitated with GluA1 antibodies, and eluted with synthetic peptides. Legends and symbols are same as , A and B. C , shown is a summary of MS/MS analysis with a protein band Cx_6 that was excised from the protein gel in A. Most of peptide sequences were matched to the sequence of either ERC2/CAST1 (#1, #2, #5, #6, and #7) or GEF-H1 (#9, #10, #11, and #12).

Article Snippet: Except one for ERC2, commercial primary antibodies were used for staining: Zizimin1 (Santa Cruz), ZO-1 (Santa Cruz), MAP2 (PCK-554P, Covance), GluA1 (Millipore).

Techniques: Immunoprecipitation, Liquid Chromatography with Mass Spectroscopy, Silver Staining, Affinity Purification, Chromatography, Tandem Mass Spectroscopy, Sequencing

Subcellular localization of three novel components of AMPA receptor complexes in the cultured cortical neurons. Primary neurons dissociated from cerebral cortex of embryonic age 17 days were cultured for 20–24 days and immunostained with antibodies against the three novel AMPA-R interactors (Zizimin1, ZO-1, and ERC2) and GluA1. A , co-localizations of Zizimin1, ZO-1, or ERC2 puncta with GluA1 puncta in the dendrite were labeled with arrowheads. Double arrowheads indicated partial co-localization of ERC2 puncta with GluA1 puncta that is a typical overlapping feature between a pre-synaptic and a post-synaptic protein. MAP2 was used as a neuronal dendritic marker to show the morphology of the dendrites. B , co-localizations of the three proteins with GluA1 in soma is shown. Due to the highly intensive signal of GluA1 and the three proteins in the soma, the gain of their signals in soma were adjusted to a lower level compared with that of dendrites during confocal imaging. All three proteins showed somatic puncta overlap with GluA1 puncta. Significant pools of ERC2 were localized at the nucleus. Scale bars are 2 μm ( A and B ). C , the co-localization of the three proteins with GluA1in the dendrites was analyzed quantitatively by measuring Manders' overlap coefficients that were plotted as the mean ± S.E. The bars with diagonal stripes (GluA1) showed percentage (%) of GluA1 puncta overlap with puncta of one of the three proteins. The bars with one of the three proteins (Zizimin1, ZO-1, or ERC2) showed % of its puncta overlap with GluA1 puncta. *, p < 0.05 ( n = 10), analysis of variance followed by Tukey's multiple comparison tests.

Journal: The Journal of Biological Chemistry

Article Title: Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes

doi: 10.1074/jbc.M111.336644

Figure Lengend Snippet: Subcellular localization of three novel components of AMPA receptor complexes in the cultured cortical neurons. Primary neurons dissociated from cerebral cortex of embryonic age 17 days were cultured for 20–24 days and immunostained with antibodies against the three novel AMPA-R interactors (Zizimin1, ZO-1, and ERC2) and GluA1. A , co-localizations of Zizimin1, ZO-1, or ERC2 puncta with GluA1 puncta in the dendrite were labeled with arrowheads. Double arrowheads indicated partial co-localization of ERC2 puncta with GluA1 puncta that is a typical overlapping feature between a pre-synaptic and a post-synaptic protein. MAP2 was used as a neuronal dendritic marker to show the morphology of the dendrites. B , co-localizations of the three proteins with GluA1 in soma is shown. Due to the highly intensive signal of GluA1 and the three proteins in the soma, the gain of their signals in soma were adjusted to a lower level compared with that of dendrites during confocal imaging. All three proteins showed somatic puncta overlap with GluA1 puncta. Significant pools of ERC2 were localized at the nucleus. Scale bars are 2 μm ( A and B ). C , the co-localization of the three proteins with GluA1in the dendrites was analyzed quantitatively by measuring Manders' overlap coefficients that were plotted as the mean ± S.E. The bars with diagonal stripes (GluA1) showed percentage (%) of GluA1 puncta overlap with puncta of one of the three proteins. The bars with one of the three proteins (Zizimin1, ZO-1, or ERC2) showed % of its puncta overlap with GluA1 puncta. *, p < 0.05 ( n = 10), analysis of variance followed by Tukey's multiple comparison tests.

Article Snippet: Except one for ERC2, commercial primary antibodies were used for staining: Zizimin1 (Santa Cruz), ZO-1 (Santa Cruz), MAP2 (PCK-554P, Covance), GluA1 (Millipore).

Techniques: Cell Culture, Labeling, Marker, Imaging

Subcellular localization of three novel components of AMPA receptor complexes in the brain. Rat brain lysates were fractionated through a series of centrifugation, sucrose gradient, and solubilization. Western blots including protein fractions were probed with antibodies against the three novel AMPA-R interactors. A , all three proteins are enriched in the second membrane fraction ( P2 ) that mainly includes synaptosomal and mitochondrial membrane proteins. ERC2 is enriched in the first membrane fraction ( P1 ) that mainly includes nuclear proteins. NeuN was used as a nuclear protein marker to verify the nuclear protein fraction. B , all three proteins are enriched in the synaptosomal membrane protein ( SMP ) and PSD fractions. PSD-95 and synaptophysin were used as pre-synaptic and post-synaptic protein markers, respectively, to verify the pre- and post-synaptic fractionations.

Journal: The Journal of Biological Chemistry

Article Title: Proteomic Analysis of α-Amino-3-hydroxy-5-methyl-4-isoxazole Propionate Receptor Complexes

doi: 10.1074/jbc.M111.336644

Figure Lengend Snippet: Subcellular localization of three novel components of AMPA receptor complexes in the brain. Rat brain lysates were fractionated through a series of centrifugation, sucrose gradient, and solubilization. Western blots including protein fractions were probed with antibodies against the three novel AMPA-R interactors. A , all three proteins are enriched in the second membrane fraction ( P2 ) that mainly includes synaptosomal and mitochondrial membrane proteins. ERC2 is enriched in the first membrane fraction ( P1 ) that mainly includes nuclear proteins. NeuN was used as a nuclear protein marker to verify the nuclear protein fraction. B , all three proteins are enriched in the synaptosomal membrane protein ( SMP ) and PSD fractions. PSD-95 and synaptophysin were used as pre-synaptic and post-synaptic protein markers, respectively, to verify the pre- and post-synaptic fractionations.

Article Snippet: Except one for ERC2, commercial primary antibodies were used for staining: Zizimin1 (Santa Cruz), ZO-1 (Santa Cruz), MAP2 (PCK-554P, Covance), GluA1 (Millipore).

Techniques: Centrifugation, Western Blot, Marker