erase-a-base system Search Results


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  • 91
    Promega erase a base system
    obg ( yhbZ ) subclones sequenced from both ends with the <t>Erase-A-Base</t> system. (A) All clones that conferred rescue of the rrmJ deletion phenotype contained the complete obg open reading frame. (B) All clones that did not rescue the rrmJ deletion growth defect did not contain the entire obg gene. obg is shown in complement, and deletions were from the 3′ end of the gene.
    Erase A Base System, supplied by Promega, used in various techniques. Bioz Stars score: 91/100, based on 656 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Promega erase a base system kit
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Erase A Base System Kit, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erase a base system kit/product/Promega
    Average 80 stars, based on 64 article reviews
    Price from $9.99 to $1999.99
    erase a base system kit - by Bioz Stars, 2020-08
    80/100 stars
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    85
    Promega erase a base system protocol
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Erase A Base System Protocol, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erase a base system protocol/product/Promega
    Average 85 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    erase a base system protocol - by Bioz Stars, 2020-08
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    85
    Promega exonuclease driven erase a base system
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Exonuclease Driven Erase A Base System, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/exonuclease driven erase a base system/product/Promega
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    exonuclease driven erase a base system - by Bioz Stars, 2020-08
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    92
    Promega exo iii erase a base system
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Exo Iii Erase A Base System, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    GE Healthcare erase a base kit
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Erase A Base Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/erase a base kit/product/GE Healthcare
    Average 89 stars, based on 7 article reviews
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    85
    Promega erase a base nucleotide kit
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Erase A Base Nucleotide Kit, supplied by Promega, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Promega exonucleaseiii based erase a base kit
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Exonucleaseiii Based Erase A Base Kit, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Promega exonuclease mapping erase a base kit
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Exonuclease Mapping Erase A Base Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Stratagene erase a base protocol
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Erase A Base Protocol, supplied by Stratagene, used in various techniques. Bioz Stars score: 80/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 3 article reviews
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    erase a base protocol - by Bioz Stars, 2020-08
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    80
    Promega promega s erase a base reagents
    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the <t>Erase-a-Base</t> kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h
    Promega S Erase A Base Reagents, supplied by Promega, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/promega s erase a base reagents/product/Promega
    Average 80 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    promega s erase a base reagents - by Bioz Stars, 2020-08
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    Image Search Results


    obg ( yhbZ ) subclones sequenced from both ends with the Erase-A-Base system. (A) All clones that conferred rescue of the rrmJ deletion phenotype contained the complete obg open reading frame. (B) All clones that did not rescue the rrmJ deletion growth defect did not contain the entire obg gene. obg is shown in complement, and deletions were from the 3′ end of the gene.

    Journal: Journal of Bacteriology

    Article Title: Overexpression of Two Different GTPases Rescues a Null Mutation in a Heat-Induced rRNA Methyltransferase

    doi: 10.1128/JB.184.10.2692-2698.2002

    Figure Lengend Snippet: obg ( yhbZ ) subclones sequenced from both ends with the Erase-A-Base system. (A) All clones that conferred rescue of the rrmJ deletion phenotype contained the complete obg open reading frame. (B) All clones that did not rescue the rrmJ deletion growth defect did not contain the entire obg gene. obg is shown in complement, and deletions were from the 3′ end of the gene.

    Article Snippet: To determine if the open reading frames were directly responsible for the observed rescue, we created subclones of one of the obg ( yhbZ )-containing clones by using the Erase-a-base method (Promega).

    Techniques: Clone Assay

    Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Journal: The Journal of Biological Chemistry

    Article Title: Transcriptional Regulation of Oncogenic Protein Kinase Cϵ (PKCϵ) by STAT1 and Sp1 Proteins *

    doi: 10.1074/jbc.M114.548446

    Figure Lengend Snippet: Deletional analysis of the human PRKCE promoter. MCF-7 cells were co-transfected with pGL3 vectors coding different PKCϵ promoter fragments generated with the Erase-a-Base kit (Promega) and pRL-TK plasmid. Luciferase activity was measured 48 h

    Article Snippet: The pGL3−1416/+219 vector was used as a template to generate a series of PRKCE promoter truncated luciferase reporter vectors (−1319/+219, −1224/+219, −1121/+219, −1032/+219, −1028/+219, −921/+219, −887/+219, −873/+219, −819/+219, −796/+219, and −777/+219) with the Erase-a-Base kit (Promega, Madison, WI). pGL3−644/+219 was generated by digestion of pGL3−808/+219 vector with PfIMI and NheI and subsequent religation.

    Techniques: Transfection, Generated, Plasmid Preparation, Luciferase, Activity Assay

    Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Journal: F1000Research

    Article Title: Identification of two telomere-proximal fission yeast DNA replication origins constrained by nearby cis-acting sequences to replicate in late S phase

    doi: 10.12688/f1000research.1-58.v1

    Figure Lengend Snippet: Exonuclease III deletions of Region 1. ( A ) Intact Region 1 with AT content, expanded from Figure 2 . ( B ) For constructs E1–E6, the thin lines show the portions of R1 that were deleted, and the thick lines show the portions of R1 remaining in each construct. The deletions were constructed using the Promega Erase-A-Base Kit. See Materials and Methods for details. ( C ) Results of ARS assays for the plasmids containing the deleted versions of Region 1 shown in panel B. Compare with Figure 2D .

    Article Snippet: The Promega Erase-a-Base Kit, which employs exonuclease III, was used to make the deletions indicated in .

    Techniques: Construct