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  • 85
    Millipore 2 ep 2 ep
    DNA fragmentation analysis showed bands at 200-bp intervals in DNA from cells treated with 2-EP at 20 μM (lane 2). The untreated DNA showed little fragmentation (lane 1). The marker showed bands at 100-bp intervals (M). 2-EP, 2-ethylpyridine. 1, untreated control sample; 2, cultures treated 24 h with 20 μM 2-EP; M, Marker
    2 Ep 2 Ep, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/2 ep 2 ep/product/Millipore
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    2 ep 2 ep - by Bioz Stars, 2020-07
    85/100 stars
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    95
    Qiagen magattract powersoil dna ep kit
    DNA fragmentation analysis showed bands at 200-bp intervals in DNA from cells treated with 2-EP at 20 μM (lane 2). The untreated DNA showed little fragmentation (lane 1). The marker showed bands at 100-bp intervals (M). 2-EP, 2-ethylpyridine. 1, untreated control sample; 2, cultures treated 24 h with 20 μM 2-EP; M, Marker
    Magattract Powersoil Dna Ep Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 95/100, based on 59 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magattract powersoil dna ep kit/product/Qiagen
    Average 95 stars, based on 59 article reviews
    Price from $9.99 to $1999.99
    magattract powersoil dna ep kit - by Bioz Stars, 2020-07
    95/100 stars
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    94
    Qiagen magattract powermicrobiome dna rna ep
    DNA fragmentation analysis showed bands at 200-bp intervals in DNA from cells treated with 2-EP at 20 μM (lane 2). The untreated DNA showed little fragmentation (lane 1). The marker showed bands at 100-bp intervals (M). 2-EP, 2-ethylpyridine. 1, untreated control sample; 2, cultures treated 24 h with 20 μM 2-EP; M, Marker
    Magattract Powermicrobiome Dna Rna Ep, supplied by Qiagen, used in various techniques. Bioz Stars score: 94/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/magattract powermicrobiome dna rna ep/product/Qiagen
    Average 94 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    magattract powermicrobiome dna rna ep - by Bioz Stars, 2020-07
    94/100 stars
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    88
    Unigene ep contig 349 ep unigene 4
    DNA fragmentation analysis showed bands at 200-bp intervals in DNA from cells treated with 2-EP at 20 μM (lane 2). The untreated DNA showed little fragmentation (lane 1). The marker showed bands at 100-bp intervals (M). 2-EP, 2-ethylpyridine. 1, untreated control sample; 2, cultures treated 24 h with 20 μM 2-EP; M, Marker
    Ep Contig 349 Ep Unigene 4, supplied by Unigene, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 5 article reviews
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    ep contig 349 ep unigene 4 - by Bioz Stars, 2020-07
    88/100 stars
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    88
    Trinity Biotech breen ep
    DNA fragmentation analysis showed bands at 200-bp intervals in DNA from cells treated with 2-EP at 20 μM (lane 2). The untreated DNA showed little fragmentation (lane 1). The marker showed bands at 100-bp intervals (M). 2-EP, 2-ethylpyridine. 1, untreated control sample; 2, cultures treated 24 h with 20 μM 2-EP; M, Marker
    Breen Ep, supplied by Trinity Biotech, used in various techniques. Bioz Stars score: 88/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/breen ep/product/Trinity Biotech
    Average 88 stars, based on 37 article reviews
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    breen ep - by Bioz Stars, 2020-07
    88/100 stars
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    92
    Cellectis dermavax ep
    Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by <t>Dermavax</t> (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p
    Dermavax Ep, supplied by Cellectis, used in various techniques. Bioz Stars score: 92/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dermavax ep/product/Cellectis
    Average 92 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    dermavax ep - by Bioz Stars, 2020-07
    92/100 stars
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    85
    Alpha Innotech ep alphaimager
    Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by <t>Dermavax</t> (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p
    Ep Alphaimager, supplied by Alpha Innotech, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ep alphaimager/product/Alpha Innotech
    Average 85 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    ep alphaimager - by Bioz Stars, 2020-07
    85/100 stars
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    85
    GE Healthcare eps 601
    Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by <t>Dermavax</t> (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p
    Eps 601, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eps 601/product/GE Healthcare
    Average 85 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    eps 601 - by Bioz Stars, 2020-07
    85/100 stars
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    92
    GE Healthcare hbs ep
    Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by <t>Dermavax</t> (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p
    Hbs Ep, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 968 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hbs ep/product/GE Healthcare
    Average 92 stars, based on 968 article reviews
    Price from $9.99 to $1999.99
    hbs ep - by Bioz Stars, 2020-07
    92/100 stars
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    92
    Abcam ep 1819y
    Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by <t>Dermavax</t> (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p
    Ep 1819y, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ep 1819y/product/Abcam
    Average 92 stars, based on 10 article reviews
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    ep 1819y - by Bioz Stars, 2020-07
    92/100 stars
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    90
    Philips Healthcare ep navigator
    Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by <t>Dermavax</t> (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p
    Ep Navigator, supplied by Philips Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ep navigator/product/Philips Healthcare
    Average 90 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    ep navigator - by Bioz Stars, 2020-07
    90/100 stars
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    92
    Biacore hbs ep
    Referenced SPR sensorgrams obtained in the Biacore 3000 with E2 concentrations ranging from 0 to 20 ng/mL. The standard solutions in 20% methanol and the wt-ERα LBD in <t>HBS-EP</t> (10 mM <t>Hepes,</t> 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20) buffer were transferred and mixed in the Biacore (1:1; v / v ). Of this mixture, 90 μL was injected over the peptide-coated sensor surface at a flow rate of 30 μL/min using HBS-EP as running buffer. After the injections, the responses were measured (dotted line) and used for the construction of the calibration curve (insert). The binding of the ER was regenerated by the injection of 10 mM NaOH for 0.5 min. The total time of each cycle was 11 min of which 5 min were used for the transfer and mixing.
    Hbs Ep, supplied by Biacore, used in various techniques. Bioz Stars score: 92/100, based on 393 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 393 article reviews
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    hbs ep - by Bioz Stars, 2020-07
    92/100 stars
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    92
    BioCore hbs ep
    Referenced SPR sensorgrams obtained in the Biacore 3000 with E2 concentrations ranging from 0 to 20 ng/mL. The standard solutions in 20% methanol and the wt-ERα LBD in <t>HBS-EP</t> (10 mM <t>Hepes,</t> 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20) buffer were transferred and mixed in the Biacore (1:1; v / v ). Of this mixture, 90 μL was injected over the peptide-coated sensor surface at a flow rate of 30 μL/min using HBS-EP as running buffer. After the injections, the responses were measured (dotted line) and used for the construction of the calibration curve (insert). The binding of the ER was regenerated by the injection of 10 mM NaOH for 0.5 min. The total time of each cycle was 11 min of which 5 min were used for the transfer and mixing.
    Hbs Ep, supplied by BioCore, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    hbs ep - by Bioz Stars, 2020-07
    92/100 stars
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    90
    Eppendorf AG mastercycler ep
    Referenced SPR sensorgrams obtained in the Biacore 3000 with E2 concentrations ranging from 0 to 20 ng/mL. The standard solutions in 20% methanol and the wt-ERα LBD in <t>HBS-EP</t> (10 mM <t>Hepes,</t> 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20) buffer were transferred and mixed in the Biacore (1:1; v / v ). Of this mixture, 90 μL was injected over the peptide-coated sensor surface at a flow rate of 30 μL/min using HBS-EP as running buffer. After the injections, the responses were measured (dotted line) and used for the construction of the calibration curve (insert). The binding of the ER was regenerated by the injection of 10 mM NaOH for 0.5 min. The total time of each cycle was 11 min of which 5 min were used for the transfer and mixing.
    Mastercycler Ep, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 90/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mastercycler ep/product/Eppendorf AG
    Average 90 stars, based on 320 article reviews
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    mastercycler ep - by Bioz Stars, 2020-07
    90/100 stars
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    93
    GE Healthcare 1xhbs ep
    Referenced SPR sensorgrams obtained in the Biacore 3000 with E2 concentrations ranging from 0 to 20 ng/mL. The standard solutions in 20% methanol and the wt-ERα LBD in <t>HBS-EP</t> (10 mM <t>Hepes,</t> 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20) buffer were transferred and mixed in the Biacore (1:1; v / v ). Of this mixture, 90 μL was injected over the peptide-coated sensor surface at a flow rate of 30 μL/min using HBS-EP as running buffer. After the injections, the responses were measured (dotted line) and used for the construction of the calibration curve (insert). The binding of the ER was regenerated by the injection of 10 mM NaOH for 0.5 min. The total time of each cycle was 11 min of which 5 min were used for the transfer and mixing.
    1xhbs Ep, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1xhbs ep/product/GE Healthcare
    Average 93 stars, based on 5 article reviews
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    1xhbs ep - by Bioz Stars, 2020-07
    93/100 stars
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    85
    Millipore ep icd
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Ep Icd, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ep icd/product/Millipore
    Average 85 stars, based on 13 article reviews
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    ep icd - by Bioz Stars, 2020-07
    85/100 stars
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    85
    GE Healthcare eps 301
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Eps 301, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/eps 301/product/GE Healthcare
    Average 85 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    eps 301 - by Bioz Stars, 2020-07
    85/100 stars
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    90
    Koehler Instrument nikonowicz ep
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Nikonowicz Ep, supplied by Koehler Instrument, used in various techniques. Bioz Stars score: 90/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nikonowicz ep/product/Koehler Instrument
    Average 90 stars, based on 20 article reviews
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    nikonowicz ep - by Bioz Stars, 2020-07
    90/100 stars
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    85
    Beckman Coulter elite eps
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Elite Eps, supplied by Beckman Coulter, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elite eps/product/Beckman Coulter
    Average 85 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    elite eps - by Bioz Stars, 2020-07
    85/100 stars
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    88
    Eppendorf AG ep realplex
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Ep Realplex, supplied by Eppendorf AG, used in various techniques. Bioz Stars score: 88/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 12 article reviews
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    ep realplex - by Bioz Stars, 2020-07
    88/100 stars
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    93
    Boehringer Ingelheim usp ep
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Usp Ep, supplied by Boehringer Ingelheim, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/usp ep/product/Boehringer Ingelheim
    Average 93 stars, based on 3 article reviews
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    usp ep - by Bioz Stars, 2020-07
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    88
    Myriad Genetics endopredict ep
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Endopredict Ep, supplied by Myriad Genetics, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 88 stars, based on 3 article reviews
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    endopredict ep - by Bioz Stars, 2020-07
    88/100 stars
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    90
    Millipore ep nps
    Fluorescence immunostaining with anti-EpEx and <t>anti-Ep-ICD</t> antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and <t>TRITC-anti-rabbit</t> (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.
    Ep Nps, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 5 article reviews
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    ep nps - by Bioz Stars, 2020-07
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    DNA fragmentation analysis showed bands at 200-bp intervals in DNA from cells treated with 2-EP at 20 μM (lane 2). The untreated DNA showed little fragmentation (lane 1). The marker showed bands at 100-bp intervals (M). 2-EP, 2-ethylpyridine. 1, untreated control sample; 2, cultures treated 24 h with 20 μM 2-EP; M, Marker

    Journal: Indian Journal of Ophthalmology

    Article Title: 2-ethylpyridine, a cigarette smoke component, causes mitochondrial damage in human retinal pigment epithelial cells in vitro

    doi: 10.4103/0301-4738.126168

    Figure Lengend Snippet: DNA fragmentation analysis showed bands at 200-bp intervals in DNA from cells treated with 2-EP at 20 μM (lane 2). The untreated DNA showed little fragmentation (lane 1). The marker showed bands at 100-bp intervals (M). 2-EP, 2-ethylpyridine. 1, untreated control sample; 2, cultures treated 24 h with 20 μM 2-EP; M, Marker

    Article Snippet: Exposure to 2-EP 2-EP was purchased from Sigma Aldrich Inc., St. Louis, MO and the stock 100 mM solution was prepared in 1 ml of deionized distilled water.

    Techniques: Marker

    Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by Dermavax (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p

    Journal: Scientific Reports

    Article Title: Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity

    doi: 10.1038/s41598-018-26281-z

    Figure Lengend Snippet: Effector immune response evaluated in vivo by “antigen challenge” in a prime-boost Luc/RT immunization of mice. BALB/c mice (n = 6) were immunized with two intradermal injections (29G needle) containing 40 µg RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by Dermavax (standard protocol) and 4 weeks later boosted with 20 µg of the same RT genes per mouse mixed 1:1 (w/w) with a Luc-encoding plasmid. Control mice received empty vector as a prime and a vector/Luc gene mixture as a boost. The emitted bioluminescence was monitored on days 1, 3, 9, 15, and 21 after boosting ( a , b ). Comparison of photon flux exhibited by mice primed with Luc/RT (Supplementary Fig. S4 ) or primed with RT and boosted with Luc/RT gene variants ( c ). Each curve ( a , c ) or bar ( b ) represents the average photon flux (photons/s/cm 2 /sr) from the injection area observed for the group of six mice (12 immunization sites), and the error bars represent the SD. The difference in photon flux between RT- and vector-immunized mice ( a , b ) and between prime and prime-boost groups of RT-immunized mice ( c ) is indicated by *p

    Article Snippet: In our previous studies, we employed Dermavax EP (Cellectis) and MN electrodes .

    Techniques: In Vivo, Mouse Assay, Electroporation, Plasmid Preparation, Injection

    Immune responses following immunization with expression-optimized RT genes. BALB/c mice (n = 5 or 6) were immunized with two intradermal injections (29G needle) containing 20 µg of RTwt, RTwt-opt, RTwt-opt-in, RT1.14, RT1.14-opt, or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by Dermavax (standard protocol) in two independent immunizations. At 21 days post-immunization mice were sacrificed, and sera and splenocytes were isolated for further immune tests. End-point average titers of anti-RT total IgG and IgG subtypes were detected using ELISA against recombinant RTwt and RT1.14 proteins with cut-offs set against the serum reactivity of control mice immunized with vector pVax 1. ( a – c ) ELISA with the identical RT protein variant received as a gene, i.e. recombinant RTwt protein for RTwt, RTwt-opt, and RTwt-opt-in immunized mice or RT1.14 for RT1.14, RT1.14-opt, and RT1.14-opt-in immunized mice. ( a ) ELISA with the homologous RT, i.e. recombinant RT1.14 protein for RTwt, RTwt-opt, and RTwt-opt-in or RTwt protein for RT1.14, RT1.14-opt, and RT1.14-opt-in immunized mice. ( b ) IgG2a/IgG1 ratio for antibody reactivity against RT variants matching RT used as DNA immunogen ( c ). In panels (a,b) the asterisk designates the difference between RTwt and RTwt-opt/RTwt-opt-in and between RT1.14 and RT1.14-opt/RT1.14-opt-in variants for all IgG subtypes. Murine splenocytes were stimulated in vitro with an RT-derived peptide representing the epitope of RT aa 528–543 (immunodominant T-cell epitope; Table 2 ) in the IFN-γ/IL-2 Fluorospot test ( d – f ). The average number of cells was registered as signal-forming units (sfu) per million splenocytes secreting IFN-γ ( d ) IL-2 ( e ) and IFN-γ/IL-2 ( f ) and the error bars represent the SD. *p

    Journal: Scientific Reports

    Article Title: Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity

    doi: 10.1038/s41598-018-26281-z

    Figure Lengend Snippet: Immune responses following immunization with expression-optimized RT genes. BALB/c mice (n = 5 or 6) were immunized with two intradermal injections (29G needle) containing 20 µg of RTwt, RTwt-opt, RTwt-opt-in, RT1.14, RT1.14-opt, or RT1.14-opt-in encoding plasmids per mouse with subsequent electroporation by Dermavax (standard protocol) in two independent immunizations. At 21 days post-immunization mice were sacrificed, and sera and splenocytes were isolated for further immune tests. End-point average titers of anti-RT total IgG and IgG subtypes were detected using ELISA against recombinant RTwt and RT1.14 proteins with cut-offs set against the serum reactivity of control mice immunized with vector pVax 1. ( a – c ) ELISA with the identical RT protein variant received as a gene, i.e. recombinant RTwt protein for RTwt, RTwt-opt, and RTwt-opt-in immunized mice or RT1.14 for RT1.14, RT1.14-opt, and RT1.14-opt-in immunized mice. ( a ) ELISA with the homologous RT, i.e. recombinant RT1.14 protein for RTwt, RTwt-opt, and RTwt-opt-in or RTwt protein for RT1.14, RT1.14-opt, and RT1.14-opt-in immunized mice. ( b ) IgG2a/IgG1 ratio for antibody reactivity against RT variants matching RT used as DNA immunogen ( c ). In panels (a,b) the asterisk designates the difference between RTwt and RTwt-opt/RTwt-opt-in and between RT1.14 and RT1.14-opt/RT1.14-opt-in variants for all IgG subtypes. Murine splenocytes were stimulated in vitro with an RT-derived peptide representing the epitope of RT aa 528–543 (immunodominant T-cell epitope; Table 2 ) in the IFN-γ/IL-2 Fluorospot test ( d – f ). The average number of cells was registered as signal-forming units (sfu) per million splenocytes secreting IFN-γ ( d ) IL-2 ( e ) and IFN-γ/IL-2 ( f ) and the error bars represent the SD. *p

    Article Snippet: In our previous studies, we employed Dermavax EP (Cellectis) and MN electrodes .

    Techniques: Expressing, Mouse Assay, Electroporation, Isolation, Enzyme-linked Immunosorbent Assay, Recombinant, Plasmid Preparation, Variant Assay, In Vitro, Derivative Assay

    RT-specific antibody and cellular responses after repeated immunization with expression-optimized RT genes. BALB/c mice (n = 5 or 6) were immunized with two intradermal injections (29G needle) containing 40 µg of RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse, with subsequent electroporation by Dermavax (standard protocol). Immunizations were repeated 4 weeks later with 20 µg of RTwt-opt-in or RT1.14-opt-in encoding plasmids (( a – e ) prime-boost regimen, “Boost” on the graph) or 5 days later with 20 µg of RTwt-opt-in encoding plasmid (( f ) double prime). Two independent immunizations were performed. At 21 days after the second immunization, mice were sacrificed and sera and splenocytes were isolated and subjected to immune tests. Endpoint average titers of anti-RT total IgG and IgG subtypes (the error bars represent the SD) were detected using ELISA against recombinant RTwt and RT1.14 proteins with cut-offs set against serum reactivity of control mice immunized with vector pVax1 ( a ), and the IgG2a/IgG1 ratio was calculated for antibody reactivity against RT variants matching RT used as the DNA immunogen ( b ). Splenocytes were stimulated in vitro with RT-derived peptides representing epitopes of RT aa 528–543 ( c – f ) and aa 465–476 (( f ) Table 2 ) or recombinant RTwt and RT1.14 proteins (( c – e ) matching the immunogen) in IFN-γ/IL-2 Fluorospot tests ( c – f ). The average number of cells was registered as signal-forming units (sfu) per million splenocytes secreting IFN-γ ( c , f ) IL-2 ( d , f ) and IFN-γ/IL-2 ( e , f ) and the error bars represent the SD. IFN-γ/IL-2 production by splenocytes of mice receiving prime-boost immunization compared to single immunization (( c – e ) single immunization from Fig. 2 ) or double prime immunization with the RTwt-opt-in gene ( f ). All assays were performed in duplicate. *p

    Journal: Scientific Reports

    Article Title: Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity

    doi: 10.1038/s41598-018-26281-z

    Figure Lengend Snippet: RT-specific antibody and cellular responses after repeated immunization with expression-optimized RT genes. BALB/c mice (n = 5 or 6) were immunized with two intradermal injections (29G needle) containing 40 µg of RTwt-opt-in or RT1.14-opt-in encoding plasmids per mouse, with subsequent electroporation by Dermavax (standard protocol). Immunizations were repeated 4 weeks later with 20 µg of RTwt-opt-in or RT1.14-opt-in encoding plasmids (( a – e ) prime-boost regimen, “Boost” on the graph) or 5 days later with 20 µg of RTwt-opt-in encoding plasmid (( f ) double prime). Two independent immunizations were performed. At 21 days after the second immunization, mice were sacrificed and sera and splenocytes were isolated and subjected to immune tests. Endpoint average titers of anti-RT total IgG and IgG subtypes (the error bars represent the SD) were detected using ELISA against recombinant RTwt and RT1.14 proteins with cut-offs set against serum reactivity of control mice immunized with vector pVax1 ( a ), and the IgG2a/IgG1 ratio was calculated for antibody reactivity against RT variants matching RT used as the DNA immunogen ( b ). Splenocytes were stimulated in vitro with RT-derived peptides representing epitopes of RT aa 528–543 ( c – f ) and aa 465–476 (( f ) Table 2 ) or recombinant RTwt and RT1.14 proteins (( c – e ) matching the immunogen) in IFN-γ/IL-2 Fluorospot tests ( c – f ). The average number of cells was registered as signal-forming units (sfu) per million splenocytes secreting IFN-γ ( c , f ) IL-2 ( d , f ) and IFN-γ/IL-2 ( e , f ) and the error bars represent the SD. IFN-γ/IL-2 production by splenocytes of mice receiving prime-boost immunization compared to single immunization (( c – e ) single immunization from Fig. 2 ) or double prime immunization with the RTwt-opt-in gene ( f ). All assays were performed in duplicate. *p

    Article Snippet: In our previous studies, we employed Dermavax EP (Cellectis) and MN electrodes .

    Techniques: Expressing, Mouse Assay, Electroporation, Plasmid Preparation, Isolation, Enzyme-linked Immunosorbent Assay, Recombinant, In Vitro, Derivative Assay

    Cellular immune responses after RT gene delivery by intradermal injections with 29 G needles or microneedles with electroporation using penetrating electrodes. BALB/c mice (n = 6–8) were immunized with two intradermal injections with a mixture of Luc/RT1.14opt-in encoding plasmids (1:1 w/w, with a total of 2 × 20 µg DNA per mouse) using an insulin syringe with a 29G needle (29G-Dermavax-MN), microneedles (Micronjet 600, Nanopass) (Microneedle-Dermavax-MN), or a Biojector 2000 (Biojector-Dermavax-MN) and electroporated using Dermavax with multi-needle (MN) electrodes or injected with a mixture of Luc/RT1.14-opt-in encoding plasmids using an insulin syringe with a 29G needle and electroporated with the BEX machine and flat electrodes (29G-BEX-FL). At 21 days post immunization, mice were sacrificed and splenocytes were isolated and subjected to in vitro stimulation with peptide representing aa 528–543 of HIV-1 RT, and cytokine secretion was assessed by dual IFN-γ/IL-2 Fluorospot. The results are shown as the average number of cells, registered as signal-forming units (sfu) per million splenocytes secreting IFN-γ ( a ) IL-2 ( b ) and IFN-γ/IL-2 ( c ) and the error bars represent the SD. All assays were performed in duplicate. *p

    Journal: Scientific Reports

    Article Title: Codon optimization and improved delivery/immunization regimen enhance the immune response against wild-type and drug-resistant HIV-1 reverse transcriptase, preserving its Th2-polarity

    doi: 10.1038/s41598-018-26281-z

    Figure Lengend Snippet: Cellular immune responses after RT gene delivery by intradermal injections with 29 G needles or microneedles with electroporation using penetrating electrodes. BALB/c mice (n = 6–8) were immunized with two intradermal injections with a mixture of Luc/RT1.14opt-in encoding plasmids (1:1 w/w, with a total of 2 × 20 µg DNA per mouse) using an insulin syringe with a 29G needle (29G-Dermavax-MN), microneedles (Micronjet 600, Nanopass) (Microneedle-Dermavax-MN), or a Biojector 2000 (Biojector-Dermavax-MN) and electroporated using Dermavax with multi-needle (MN) electrodes or injected with a mixture of Luc/RT1.14-opt-in encoding plasmids using an insulin syringe with a 29G needle and electroporated with the BEX machine and flat electrodes (29G-BEX-FL). At 21 days post immunization, mice were sacrificed and splenocytes were isolated and subjected to in vitro stimulation with peptide representing aa 528–543 of HIV-1 RT, and cytokine secretion was assessed by dual IFN-γ/IL-2 Fluorospot. The results are shown as the average number of cells, registered as signal-forming units (sfu) per million splenocytes secreting IFN-γ ( a ) IL-2 ( b ) and IFN-γ/IL-2 ( c ) and the error bars represent the SD. All assays were performed in duplicate. *p

    Article Snippet: In our previous studies, we employed Dermavax EP (Cellectis) and MN electrodes .

    Techniques: Electroporation, Mouse Assay, Injection, Isolation, In Vitro

    Referenced SPR sensorgrams obtained in the Biacore 3000 with E2 concentrations ranging from 0 to 20 ng/mL. The standard solutions in 20% methanol and the wt-ERα LBD in HBS-EP (10 mM Hepes, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20) buffer were transferred and mixed in the Biacore (1:1; v / v ). Of this mixture, 90 μL was injected over the peptide-coated sensor surface at a flow rate of 30 μL/min using HBS-EP as running buffer. After the injections, the responses were measured (dotted line) and used for the construction of the calibration curve (insert). The binding of the ER was regenerated by the injection of 10 mM NaOH for 0.5 min. The total time of each cycle was 11 min of which 5 min were used for the transfer and mixing.

    Journal: Biosensors

    Article Title: Label-Free Biosensor Detection of Endocrine Disrupting Compounds Using Engineered Estrogen Receptors

    doi: 10.3390/bios8010001

    Figure Lengend Snippet: Referenced SPR sensorgrams obtained in the Biacore 3000 with E2 concentrations ranging from 0 to 20 ng/mL. The standard solutions in 20% methanol and the wt-ERα LBD in HBS-EP (10 mM Hepes, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20) buffer were transferred and mixed in the Biacore (1:1; v / v ). Of this mixture, 90 μL was injected over the peptide-coated sensor surface at a flow rate of 30 μL/min using HBS-EP as running buffer. After the injections, the responses were measured (dotted line) and used for the construction of the calibration curve (insert). The binding of the ER was regenerated by the injection of 10 mM NaOH for 0.5 min. The total time of each cycle was 11 min of which 5 min were used for the transfer and mixing.

    Article Snippet: The final optimized assay conditions were as follows: 100 μL of the wt-ERαLBD (60 nM) in HBS-EP (10 mM Hepes, 150 mM NaCl, 3 mM EDTA, 0.005% Tween-20) buffer was mixed in the Biacore with 100 μL of E2 standard/sample in 20% methanol and of this mixture 90 μL was injected at 30 μL/min with HBS-EP as running buffer which was followed by the injection of 10 mM NaOH for 0.5 min for the regeneration.

    Techniques: SPR Assay, Injection, Flow Cytometry, Binding Assay

    Fluorescence immunostaining with anti-EpEx and anti-Ep-ICD antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and TRITC-anti-rabbit (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.

    Journal: PLoS ONE

    Article Title: An Ep-ICD Based Index Is a Marker of Aggressiveness and Poor Prognosis in Thyroid Carcinoma

    doi: 10.1371/journal.pone.0042893

    Figure Lengend Snippet: Fluorescence immunostaining with anti-EpEx and anti-Ep-ICD antibodies in aggressive and non-aggressive papillary thyroid carcinomas. Secondary antibodies are FITC-anti-mouse (green) and TRITC-anti-rabbit (red). A-F images from a non-aggressive PTC; G-L Images from an aggressive PTC. A,G) EpEx; B,H) DAPI; C, I) Ep-ICD; D) EpEx and DAPI (A C merged); E) Ep-ICD and DAPI (B C merged); F) EpEx, Ep-ICD, and DAPI (A, B, C merged). J) EpEx and DAPI (G I merged); K) Ep-ICD and DAPI (H I merged); L) EpEx, Ep-ICD, and DAPI (G, H, I merged). M, Membranous staining; C, Cytoplasm staining; N, Nuclear staining. Original magnification × 400.

    Article Snippet: Immunofluorescence Analysis of Ep-ICD and EpEx Localization in Thyroid Carcinomas Immunofluorescence analysis was carried out using a TRITC-labeled goat anti-rabbit secondary antibody for detecting Ep-ICD (Sigma-Aldrich, 1∶200 dilution) and a FITC-labelled goat anti-mouse secondary antibody (Sigma-Aldrich, St. Louis, MO, 1∶200 dilution) for detecting EpEx.

    Techniques: Fluorescence, Immunostaining, Staining

    Scatter plot analysis of membrane EpEx, Ep-ICD and ESLI expression in thyroid tumors. Scatter plots show the distribution of total IHC scores determined by immunohistochemical analysis of tissue sections from benign thyroid nodules (n = 16), non-aggressive PTC (n = 63) and aggressive PTC cases (n = 121). The vertical axis gives the immunohistochemical staining score as described in the Methods section. The horizontal bars are the cut-off IHC score threshold derived from the relevant ROC curves to classify aggressive PTC cases from non-aggressive PTC cases with sensitivities and specificities summarized in Table 4 . Each point represents an average IHC score of five stained fields in each tissue. The red/green lines show mean ± standard error of mean (SEM) values for the markers analyzed. High membrane EpEx expression was observed in all of the benign cases and most of the non-aggressive PTC cases (A). Decreased membrane expression of EpEx was observed in most of the aggressive PTC cases analyzed (B). Increased cytoplasmic (C) and nuclear expression (D) of Ep-ICD was observed in aggressive PTCs as compared to the benign and non-aggressive PTC groups. An increasing trend of ESLI was observed across the three groups of patients correlating with aggressiveness of tumors (E). BN, benign; AGPTC, aggressive PTC; NAGPTC, non-aggressive PTC.

    Journal: PLoS ONE

    Article Title: An Ep-ICD Based Index Is a Marker of Aggressiveness and Poor Prognosis in Thyroid Carcinoma

    doi: 10.1371/journal.pone.0042893

    Figure Lengend Snippet: Scatter plot analysis of membrane EpEx, Ep-ICD and ESLI expression in thyroid tumors. Scatter plots show the distribution of total IHC scores determined by immunohistochemical analysis of tissue sections from benign thyroid nodules (n = 16), non-aggressive PTC (n = 63) and aggressive PTC cases (n = 121). The vertical axis gives the immunohistochemical staining score as described in the Methods section. The horizontal bars are the cut-off IHC score threshold derived from the relevant ROC curves to classify aggressive PTC cases from non-aggressive PTC cases with sensitivities and specificities summarized in Table 4 . Each point represents an average IHC score of five stained fields in each tissue. The red/green lines show mean ± standard error of mean (SEM) values for the markers analyzed. High membrane EpEx expression was observed in all of the benign cases and most of the non-aggressive PTC cases (A). Decreased membrane expression of EpEx was observed in most of the aggressive PTC cases analyzed (B). Increased cytoplasmic (C) and nuclear expression (D) of Ep-ICD was observed in aggressive PTCs as compared to the benign and non-aggressive PTC groups. An increasing trend of ESLI was observed across the three groups of patients correlating with aggressiveness of tumors (E). BN, benign; AGPTC, aggressive PTC; NAGPTC, non-aggressive PTC.

    Article Snippet: Immunofluorescence Analysis of Ep-ICD and EpEx Localization in Thyroid Carcinomas Immunofluorescence analysis was carried out using a TRITC-labeled goat anti-rabbit secondary antibody for detecting Ep-ICD (Sigma-Aldrich, 1∶200 dilution) and a FITC-labelled goat anti-mouse secondary antibody (Sigma-Aldrich, St. Louis, MO, 1∶200 dilution) for detecting EpEx.

    Techniques: Expressing, Immunohistochemistry, Staining, Derivative Assay

    Immunohistochemical analysis of EpEx and Ep-ICD expression in papillary thyroid carcinomas and benign tissues. The representative photomicrographs show immunostaining of EpEx and Ep-ICD in paraffin-embedded thyroid benign nodule goiters, non-aggressive PTC and aggressive PTC tissues. Strong membranous EpEx immunostaining was observed in benign cases (A) and non-aggressive PTC tissues (C); reduced staining of membrane EpEx was observed in aggressive PTC cases (E, G). The benign thyroid nodules and non-aggressive PTC (D) showed predominant cytoplasm localization of Ep-ICD and no detectable nuclear Ep-ICD staining (B, D), while the aggressive PTC cases showed strong nuclear and cytoplasmic Ep-ICD accumulation (F, H). M, membrane staining; C, cytoplasmic staining; N, nuclear staining; Loss of M, loss of membrane expression. Original magnification × 400.

    Journal: PLoS ONE

    Article Title: An Ep-ICD Based Index Is a Marker of Aggressiveness and Poor Prognosis in Thyroid Carcinoma

    doi: 10.1371/journal.pone.0042893

    Figure Lengend Snippet: Immunohistochemical analysis of EpEx and Ep-ICD expression in papillary thyroid carcinomas and benign tissues. The representative photomicrographs show immunostaining of EpEx and Ep-ICD in paraffin-embedded thyroid benign nodule goiters, non-aggressive PTC and aggressive PTC tissues. Strong membranous EpEx immunostaining was observed in benign cases (A) and non-aggressive PTC tissues (C); reduced staining of membrane EpEx was observed in aggressive PTC cases (E, G). The benign thyroid nodules and non-aggressive PTC (D) showed predominant cytoplasm localization of Ep-ICD and no detectable nuclear Ep-ICD staining (B, D), while the aggressive PTC cases showed strong nuclear and cytoplasmic Ep-ICD accumulation (F, H). M, membrane staining; C, cytoplasmic staining; N, nuclear staining; Loss of M, loss of membrane expression. Original magnification × 400.

    Article Snippet: Immunofluorescence Analysis of Ep-ICD and EpEx Localization in Thyroid Carcinomas Immunofluorescence analysis was carried out using a TRITC-labeled goat anti-rabbit secondary antibody for detecting Ep-ICD (Sigma-Aldrich, 1∶200 dilution) and a FITC-labelled goat anti-mouse secondary antibody (Sigma-Aldrich, St. Louis, MO, 1∶200 dilution) for detecting EpEx.

    Techniques: Immunohistochemistry, Expressing, Immunostaining, Staining