epididymal adipose tissues Search Results


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  • 99
    Thermo Fisher flow cytometry epididymal adipose tissues
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Flow Cytometry Epididymal Adipose Tissues, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore theophylline
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
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    Millipore epididymal adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Epididymal Adipose Tissue, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 171 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher epididymal adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Epididymal Adipose Tissue, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 333 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare epididymal adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Epididymal Adipose Tissue, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Nikon epididymal white adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Epididymal White Adipose Tissue, supplied by Nikon, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    FUJIFILM histology epididymal adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Histology Epididymal Adipose Tissue, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 88/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Molecular Biosciences Inc epididymal white adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Epididymal White Adipose Tissue, supplied by Molecular Biosciences Inc, used in various techniques. Bioz Stars score: 88/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioLegend legendplex total epididymal adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Legendplex Total Epididymal Adipose Tissue, supplied by BioLegend, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa epididymal adipose tissue total rna
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Epididymal Adipose Tissue Total Rna, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IHC World paraffin embedded epididymal adipose tissue
    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow <t>cytometry</t> analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P
    Paraffin Embedded Epididymal Adipose Tissue, supplied by IHC World, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Deltagen mouse epididymal adipose tissue explants ffa2 deficient ffa2 mice
    Effects of <t>FFA2</t> agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P
    Mouse Epididymal Adipose Tissue Explants Ffa2 Deficient Ffa2 Mice, supplied by Deltagen, used in various techniques. Bioz Stars score: 88/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen rneasy lipid tissue mini kit
    Effects of <t>FFA2</t> agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P
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    Becton Dickinson inguinal adipose tissues
    Effects of <t>FFA2</t> agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P
    Inguinal Adipose Tissues, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of <t>FFA2</t> agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P
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    Millipore eosin
    Effects of <t>FFA2</t> agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P
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    Carl Zeiss lsm 510 meta confocal scanning laser microscope
    Effects of <t>FFA2</t> agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P
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    Millipore collagenase pbs
    Effects of <t>FFA2</t> agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P
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    Image Search Results


    RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow cytometry analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P

    Journal: Nature Communications

    Article Title: Obesity-induced DNA hypermethylation of the adiponectin gene mediates insulin resistance

    doi: 10.1038/ncomms8585

    Figure Lengend Snippet: RG108 improves metabolic parameters in db/db mice. db/db mice were injected with vehicle (Veh, 1% DMSO, n =5–7) or RG108 ( n =5–7). ( a ) Fasting glucose and insulin levels in serum. ( b ) Serum TG and FFA levels. ( c ) OGTT. After 16 h fasting, Veh- or RG108-injected db/db and DKO mice were administered 1 g kg −1 body weight of glucose bolus by oral gavage and blood glucose levels were monitored. Time course of blood clearance and area under the curve (AUC) are presented. ( d , e ) Western blot of insulin signalling in liver ( d ) and skeletal muscle ( e ). Veh- or RG108-injected db/db mice were fasted for 16 h, and then 0.75 mU g− 1 body weight of insulin were intraperitoneally injected. After 30 min, livers and skeletal muscle tissues were collected. ( f ) Histological analysis of epididymal white adipose tissue (eWAT; haematoxylin and eosin (H E) staining). Scale bar, 200 μm. ( g ) Flow cytometry analysis of SVCs from eWAT. ( h ) mRNA levels of pro-inflammatory genes in SVCs. mRNA levels were measured by qPCR. ( i ) Histological analysis of the liver (H E staining). Scale bar, 200 μm. ( j ) Hepatic TG contents. ( k ) mRNA levels of lipogenic genes in the liver. mRNA levels were measured by qPCR. ( l ) Relative mRNA levels of inflammatory genes in liver. Results are expressed as the mean±s.e.m. * P

    Article Snippet: Adipose tissue fractionation and flow cytometry Epididymal adipose tissues were isolated from each mouse, rinsed in PBS, minced and digested for 35 min at 37 °C in Krebs–Ringer phosphate buffer (pH 7.4) with 2% bovine serum albumin and 1.75 mg ml−1 of type I collagenase (Gibco, 17100-017).

    Techniques: Mouse Assay, Injection, Western Blot, Staining, Flow Cytometry, Cytometry, Real-time Polymerase Chain Reaction

    Effects of FFA2 agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P

    Journal: Pharmacology Research & Perspectives

    Article Title: Pharmacological properties of acid N-thiazolylamide FFA2 agonists

    doi: 10.1002/prp2.141

    Figure Lengend Snippet: Effects of FFA2 agonists on lipolysis and GLP-1 release in mouse cells. (A) Adipose tissue explants from FFA2 −/− mice and matched wild-type littermates were treated in vitro with vehicle, C3 (20 mmol/L), 4-CMTB, or 14 (75 μ mol/L) and lipolysis determined by measurement of glycerol release. Data represent mean ± SEM ( n = 6–11 across two experiment occasions; **P

    Article Snippet: Lipolysis in mouse epididymal adipose tissue explants FFA2-deficient (FFA2−/− ) mice were obtained from Deltagen (San Mateo, CA).

    Techniques: Mouse Assay, In Vitro

    Inhibition of lipolysis by FFA2 agonists in primary human adipocytes. (A) Primary human adipocytes were treated with isoproterenol (Iso) or insulin (both at 200 nmol/L) or FFA2 agonists (10–100 μ mol/L; only 50 μ mol/L treatment is shown) and lipolysis determined as above. Bars represent mean ± SD ( n = 4 determinations across two experiment occasions). (B) Primary human adipocytes were pretreated with PTX or vehicle before drug treatment. Bars represent mean ± SD from three experiments with each condition determined in duplicate in each experiment. (C) Primary human adipocytes were pretreated with the hFFA2 antagonist N -CBT (10–50 μ mol/L) prior to 4-CMTB treatment. Data show mean ± SD from two experiments, each condition was determined n = 2–4 per experiment. *P

    Journal: Pharmacology Research & Perspectives

    Article Title: Pharmacological properties of acid N-thiazolylamide FFA2 agonists

    doi: 10.1002/prp2.141

    Figure Lengend Snippet: Inhibition of lipolysis by FFA2 agonists in primary human adipocytes. (A) Primary human adipocytes were treated with isoproterenol (Iso) or insulin (both at 200 nmol/L) or FFA2 agonists (10–100 μ mol/L; only 50 μ mol/L treatment is shown) and lipolysis determined as above. Bars represent mean ± SD ( n = 4 determinations across two experiment occasions). (B) Primary human adipocytes were pretreated with PTX or vehicle before drug treatment. Bars represent mean ± SD from three experiments with each condition determined in duplicate in each experiment. (C) Primary human adipocytes were pretreated with the hFFA2 antagonist N -CBT (10–50 μ mol/L) prior to 4-CMTB treatment. Data show mean ± SD from two experiments, each condition was determined n = 2–4 per experiment. *P

    Article Snippet: Lipolysis in mouse epididymal adipose tissue explants FFA2-deficient (FFA2−/− ) mice were obtained from Deltagen (San Mateo, CA).

    Techniques: Inhibition

    Structures of N -thiazolylamide and synthetic FFA2 ligands used in this study.

    Journal: Pharmacology Research & Perspectives

    Article Title: Pharmacological properties of acid N-thiazolylamide FFA2 agonists

    doi: 10.1002/prp2.141

    Figure Lengend Snippet: Structures of N -thiazolylamide and synthetic FFA2 ligands used in this study.

    Article Snippet: Lipolysis in mouse epididymal adipose tissue explants FFA2-deficient (FFA2−/− ) mice were obtained from Deltagen (San Mateo, CA).

    Techniques: