enzyme-linked immunosorbent assay elisa Search Results


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  • 99
    Thermo Fisher enzyme linked immunosorbent assay elisa
    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Data are shown as the mean ± s.e.m. of three independent experiments.
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 234 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenWay enzyme linked immunosorbant assay elisa
    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Data are shown as the mean ± s.e.m. of three independent experiments.
    Enzyme Linked Immunosorbant Assay Elisa, supplied by GenWay, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Genzyme enzyme linked immunosorbant assay elisa
    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Data are shown as the mean ± s.e.m. of three independent experiments.
    Enzyme Linked Immunosorbant Assay Elisa, supplied by Genzyme, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ZeptoMetrix enzyme linked immunosorbent assay elisa
    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Data are shown as the mean ± s.e.m. of three independent experiments.
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    BioPorto enzyme linked immunosorbent assay elisa
    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Data are shown as the mean ± s.e.m. of three independent experiments.
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    Pharmingen enzyme linked immunosorbent assays elisa
    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Data are shown as the mean ± s.e.m. of three independent experiments.
    Enzyme Linked Immunosorbent Assays Elisa, supplied by Pharmingen, used in various techniques. Bioz Stars score: 93/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems enzyme linked immunosorbent assay elisa
    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.
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    ALPCO enzyme linked immunosorbent assay elisa
    Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .
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    Endogen Inc enzyme linked immunosorbent assay elisa
    Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .
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    GE Healthcare enzyme linked immunosorbent assay elisa
    Evidence of induction of alloproliferative hyporesponsiveness by treadmill exercise. (a) Results of cell proliferation assays in mixed leukocyte cultures (MLCs). (b–e) MLC studies of cytokines. Levels of interleukin (IL)-4 (b), IL-10 (c), IL-2 (d), and interferon-γ (e) in MLCs were assessed by enzyme-linked <t>immunosorbent</t> assays <t>(ELISA).</t> (f) Serum concentration of IL-10. Levels of IL-10 in the serum of CBA recipients with/without postoperative 1-week treadmill exercise and naïve CBA mice were assessed by ELISA. The data shown are mean ± SD values obtained in one representative experiment because similar results were achieved in three independent experiments. * P
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    Bio-Rad enzyme linked immunosorbent assay elisa
    Effects of TA on TNF-α, IL-6 and IL-1β production in RVSMCs induced with H 2 O 2 . RVSMCs were pretreated with various concentrations of TA (0, 12.5, 25 and 50 µ M) for 2 h, followed by treatment with H 2 O 2 (final concentration 100 µ M) for a further 24 h. Enzyme-linked <t>immunosorbent</t> assay was performed to quantify (A) TNF-α, (B) IL-6 and (C) IL-1β levels. Data are presented as the mean ± standard deviation obtained from five individual experiments performed in triplicate. * P
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    EUROIMMUN enzyme linked immunosorbent assay elisa
    Effects of TA on TNF-α, IL-6 and IL-1β production in RVSMCs induced with H 2 O 2 . RVSMCs were pretreated with various concentrations of TA (0, 12.5, 25 and 50 µ M) for 2 h, followed by treatment with H 2 O 2 (final concentration 100 µ M) for a further 24 h. Enzyme-linked <t>immunosorbent</t> assay was performed to quantify (A) TNF-α, (B) IL-6 and (C) IL-1β levels. Data are presented as the mean ± standard deviation obtained from five individual experiments performed in triplicate. * P
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    BioLegend enzyme linked immunosorbent assay elisa
    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P
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    Millipore elisa enzyme linked immunosorbent assays
    Neonatal wounding induces the production and secretion of neurotrophin-3 (NT-3) in skin. (A) Enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> of NT-3 protein concentration in rat skin at the indicated times following P0 skin wounding (n = 6-8 for each age and condition). ∗∗ P
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    Reliatech enzyme linked immunosorbent assay elisa
    Neonatal wounding induces the production and secretion of neurotrophin-3 (NT-3) in skin. (A) Enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> of NT-3 protein concentration in rat skin at the indicated times following P0 skin wounding (n = 6-8 for each age and condition). ∗∗ P
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    PerkinElmer enzyme linked immunosorbent assay elisa
    Experimental workflow and number of wells tested by all methods. A, Processing of peripheral blood mononuclear cells (PBMCs) by 2 methods to yield populations of CD32 hi and CD32 − CD4 + T cells for quantitative viral outgrowth assay (QVOA) (method 1, n = 3; method 2, n = 3) and additional surface marker analyses for CD14, CD19, and HLA-DR (method 2 only). B, Number of CD32 hi and CD32 − cocultured wells analyzed by standard enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> and ultrasensitive Simoa assay.
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    R&D Systems enzyme linked immunosorbant assay elisa
    Experimental workflow and number of wells tested by all methods. A, Processing of peripheral blood mononuclear cells (PBMCs) by 2 methods to yield populations of CD32 hi and CD32 − CD4 + T cells for quantitative viral outgrowth assay (QVOA) (method 1, n = 3; method 2, n = 3) and additional surface marker analyses for CD14, CD19, and HLA-DR (method 2 only). B, Number of CD32 hi and CD32 − cocultured wells analyzed by standard enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> and ultrasensitive Simoa assay.
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    Bio-Techne enzyme linked immunosorbent assay elisa
    Experimental workflow and number of wells tested by all methods. A, Processing of peripheral blood mononuclear cells (PBMCs) by 2 methods to yield populations of CD32 hi and CD32 − CD4 + T cells for quantitative viral outgrowth assay (QVOA) (method 1, n = 3; method 2, n = 3) and additional surface marker analyses for CD14, CD19, and HLA-DR (method 2 only). B, Number of CD32 hi and CD32 − cocultured wells analyzed by standard enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> and ultrasensitive Simoa assay.
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    Becton Dickinson enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 95/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Pishtaz Teb enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Bionova enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    BioTek Instruments enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    IBEX Technologies enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Diaclone enzyme linked immunosorbent elisa assay
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Sanofi enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Eagle Biosciences enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Eagle Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Adipogen enzyme linked immunosorbent elisa assays
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    DRG International enzyme linked immunosorbent assay elisa
    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Elabscience enzyme linked immunosorbent assay elisa
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    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked <t>immunosorbent</t> assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p
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    Image Search Results


    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked immunosorbent assay (ELISA). Data are shown as the mean ± s.e.m. of three independent experiments.

    Journal: Clinical and Experimental Immunology

    Article Title: Natural killer cell activities of synbiotic Lactobacillus casei ssp. casei in conjunction with dextran

    doi: 10.1111/j.1365-2249.2005.02975.x

    Figure Lengend Snippet: Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked immunosorbent assay (ELISA). Data are shown as the mean ± s.e.m. of three independent experiments.

    Article Snippet: Culture supernatants were collected and analysed by enzyme-linked immunosorbent assay (ELISA) for secreted IL-12 ( e Bioscience, San Diego, CA, USA).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked immunosorbent assay (ELISA) in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.

    Journal: Thrombosis and haemostasis

    Article Title: Increased Neutrophil Activation and Plasma DNA Levels in Patients with Pre-Eclampsia

    doi: 10.1055/s-0038-1675788

    Figure Lengend Snippet: Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked immunosorbent assay (ELISA) in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.

    Article Snippet: Plasma MPO levels were measured by enzyme-linked immunosorbent assay (ELISA) (R & D Systems, DMYE00B).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked immunosorbent assay (ELISA). Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .

    Journal: Clinical and Experimental Immunology

    Article Title: Vitamin D3 correlates inversely with systemic dendritic cell numbers and bone erosion in chronic rhinosinusitis with nasal polyps and allergic fungal rhinosinusitis

    doi: 10.1111/j.1365-2249.2011.04325.x

    Figure Lengend Snippet: Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked immunosorbent assay (ELISA). Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .

    Article Snippet: Levels of 25-dihydroxy VD3 were measured by enzyme-linked immunosorbent assay (ELISA) (Alpco Immunoassays, Salem, NH, USA) according to the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay

    Evidence of induction of alloproliferative hyporesponsiveness by treadmill exercise. (a) Results of cell proliferation assays in mixed leukocyte cultures (MLCs). (b–e) MLC studies of cytokines. Levels of interleukin (IL)-4 (b), IL-10 (c), IL-2 (d), and interferon-γ (e) in MLCs were assessed by enzyme-linked immunosorbent assays (ELISA). (f) Serum concentration of IL-10. Levels of IL-10 in the serum of CBA recipients with/without postoperative 1-week treadmill exercise and naïve CBA mice were assessed by ELISA. The data shown are mean ± SD values obtained in one representative experiment because similar results were achieved in three independent experiments. * P

    Journal: Transplant International

    Article Title: Treadmill exercise induces murine cardiac allograft survival and generates regulatory T cell

    doi: 10.1111/tri.12491

    Figure Lengend Snippet: Evidence of induction of alloproliferative hyporesponsiveness by treadmill exercise. (a) Results of cell proliferation assays in mixed leukocyte cultures (MLCs). (b–e) MLC studies of cytokines. Levels of interleukin (IL)-4 (b), IL-10 (c), IL-2 (d), and interferon-γ (e) in MLCs were assessed by enzyme-linked immunosorbent assays (ELISA). (f) Serum concentration of IL-10. Levels of IL-10 in the serum of CBA recipients with/without postoperative 1-week treadmill exercise and naïve CBA mice were assessed by ELISA. The data shown are mean ± SD values obtained in one representative experiment because similar results were achieved in three independent experiments. * P

    Article Snippet: Proliferation was assessed using an enzyme-linked immunosorbent assay (ELISA) for BrdU incorporation (Biotrak, version 2; Amersham, Little Chalfont, UK) according to the manufacturer's instructions [ ].

    Techniques: Enzyme-linked Immunosorbent Assay, Concentration Assay, Crocin Bleaching Assay, Mouse Assay

    Effects of TA on TNF-α, IL-6 and IL-1β production in RVSMCs induced with H 2 O 2 . RVSMCs were pretreated with various concentrations of TA (0, 12.5, 25 and 50 µ M) for 2 h, followed by treatment with H 2 O 2 (final concentration 100 µ M) for a further 24 h. Enzyme-linked immunosorbent assay was performed to quantify (A) TNF-α, (B) IL-6 and (C) IL-1β levels. Data are presented as the mean ± standard deviation obtained from five individual experiments performed in triplicate. * P

    Journal: Molecular Medicine Reports

    Article Title: Tormentic acid inhibits H2O2-induced oxidative stress and inflammation in rat vascular smooth muscle cells via inhibition of the NF-κB signaling pathway

    doi: 10.3892/mmr.2016.5690

    Figure Lengend Snippet: Effects of TA on TNF-α, IL-6 and IL-1β production in RVSMCs induced with H 2 O 2 . RVSMCs were pretreated with various concentrations of TA (0, 12.5, 25 and 50 µ M) for 2 h, followed by treatment with H 2 O 2 (final concentration 100 µ M) for a further 24 h. Enzyme-linked immunosorbent assay was performed to quantify (A) TNF-α, (B) IL-6 and (C) IL-1β levels. Data are presented as the mean ± standard deviation obtained from five individual experiments performed in triplicate. * P

    Article Snippet: Following the described H2 O2 and TA treatments, 10 µ l CCK-8 reagent was added to each well and the cells incubated for a further 2 h. Finally, the absorbance was read at 570 nm (A570 ) using a Bio-Rad enzyme-linked immunosorbent assay (ELISA) microplate reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA).

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Standard Deviation

    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked immunosorbent assay (ELISA) (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P

    Journal: Science Advances

    Article Title: NDR2 promotes the antiviral immune response via facilitating TRIM25-mediated RIG-I activation in macrophages

    doi: 10.1126/sciadv.aav0163

    Figure Lengend Snippet: NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked immunosorbent assay (ELISA) (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P

    Article Snippet: Enzyme-linked immunosorbent assay The protein levels of murine IFN-β, IL-6, and TNF-α in cell culture supernatants and sera were detected by enzyme-linked immunosorbent assay (ELISA) according to the manufacturers’ instructions (BioLegend and eBioscience).

    Techniques: Activity Assay, Expressing, Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Fluorescence, FACS, Transfection, Plasmid Preparation, Stable Transfection

    Neonatal wounding induces the production and secretion of neurotrophin-3 (NT-3) in skin. (A) Enzyme-linked immunosorbent assay (ELISA) of NT-3 protein concentration in rat skin at the indicated times following P0 skin wounding (n = 6-8 for each age and condition). ∗∗ P

    Journal: Pain

    Article Title: A role for NT-3 in the hyperinnervation of neonatally wounded skin

    doi: 10.1016/j.pain.2012.07.012

    Figure Lengend Snippet: Neonatal wounding induces the production and secretion of neurotrophin-3 (NT-3) in skin. (A) Enzyme-linked immunosorbent assay (ELISA) of NT-3 protein concentration in rat skin at the indicated times following P0 skin wounding (n = 6-8 for each age and condition). ∗∗ P

    Article Snippet: 2.3 ELISA Enzyme-linked immunosorbent assays (ELISAs; Chemicon; Merck Millipore, Billerica, MA, USA) were undertaken to quantify (1) NT-3 protein levels in wounded skin and (2) the amount of NT-3 protein secreted by the wounded skin.

    Techniques: Enzyme-linked Immunosorbent Assay, Protein Concentration

    Experimental workflow and number of wells tested by all methods. A, Processing of peripheral blood mononuclear cells (PBMCs) by 2 methods to yield populations of CD32 hi and CD32 − CD4 + T cells for quantitative viral outgrowth assay (QVOA) (method 1, n = 3; method 2, n = 3) and additional surface marker analyses for CD14, CD19, and HLA-DR (method 2 only). B, Number of CD32 hi and CD32 − cocultured wells analyzed by standard enzyme-linked immunosorbent assay (ELISA) and ultrasensitive Simoa assay.

    Journal: The Journal of Infectious Diseases

    Article Title: The Latent Human Immunodeficiency Virus (HIV) Reservoir Resides Primarily in CD32−CD4+ T Cells in Perinatally HIV-Infected Adolescents With Long-Term Virologic Suppression

    doi: 10.1093/infdis/jiy461

    Figure Lengend Snippet: Experimental workflow and number of wells tested by all methods. A, Processing of peripheral blood mononuclear cells (PBMCs) by 2 methods to yield populations of CD32 hi and CD32 − CD4 + T cells for quantitative viral outgrowth assay (QVOA) (method 1, n = 3; method 2, n = 3) and additional surface marker analyses for CD14, CD19, and HLA-DR (method 2 only). B, Number of CD32 hi and CD32 − cocultured wells analyzed by standard enzyme-linked immunosorbent assay (ELISA) and ultrasensitive Simoa assay.

    Article Snippet: Viral outgrowth is defined as the presence of HIV p24 at day 14 in the supernatant measured with the ultrasensitive Simoa assay (mean limit of detection, 0.003 pg/mL; limit of quantitation [LOQ], 0.01 pg/mL) (Quanterix), and at day 21 for the standard enzyme-linked immunosorbent assay (ELISA) (limit of detection, 4.3 pg/mL [ ]; LOQ, 6.25 pg/mL) (PerkinElmer).

    Techniques: Quantitative Viral Outgrowth Assay, Marker, Enzyme-linked Immunosorbent Assay

    Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked immunosorbent assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p

    Journal: Biomolecules & Therapeutics

    Article Title: Protective Effects of Chlorogenic Acid against Experimental Reflux Esophagitis in Rats

    doi: 10.4062/biomolther.2014.066

    Figure Lengend Snippet: Effects of CGA on inflammatory mediators in RE. (A) The serum concentration of TNF-α was determined using an enzyme-linked immunosorbent assay. (B) Western blot analysis for iNOS and COX-2 was performed on whole extracts from the esophagus. Results are presented as mean ± S.E.M. of 8–10 animals per group. #,## Denote significant differences ( p

    Article Snippet: Serum TNF-α The serum TNF-α level was quantified by enzyme-linked immunosorbent assay (ELISA) using a commercial TNF-α ELISA assay kit (BD Biosciences Co., SanJose, CA, USA) according to the manufacturer’s instructions.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Western Blot