enzyme-linked immunosorbent assay elisa Search Results


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  • 94
    Abcam enzyme linked immunosorbent assay elisa
    MS-444, a small molecule inhibitor of human antigen R (HuR), attenuates cytokine messenger RNA expression in stretch-injured astrocytes. (A) Twenty-four hours after injury, RNA levels were measured by quantitative real-time polymerase chain reaction analysis. (B) Enzyme-linked <t>immunosorbent</t> assay analysis of conditioned media from astrocytes shown in (A). All data points are the mean ± standard error of the mean of six independent samples, and values were expressed as a relative quantity (RQ) to the vehicle control. * p
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    Immunotec enzyme linked immunosorbent assay elisa
    MS-444, a small molecule inhibitor of human antigen R (HuR), attenuates cytokine messenger RNA expression in stretch-injured astrocytes. (A) Twenty-four hours after injury, RNA levels were measured by quantitative real-time polymerase chain reaction analysis. (B) Enzyme-linked <t>immunosorbent</t> assay analysis of conditioned media from astrocytes shown in (A). All data points are the mean ± standard error of the mean of six independent samples, and values were expressed as a relative quantity (RQ) to the vehicle control. * p
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    Elabscience enzyme linked immunosorbent assay elisa
    MS-444, a small molecule inhibitor of human antigen R (HuR), attenuates cytokine messenger RNA expression in stretch-injured astrocytes. (A) Twenty-four hours after injury, RNA levels were measured by quantitative real-time polymerase chain reaction analysis. (B) Enzyme-linked <t>immunosorbent</t> assay analysis of conditioned media from astrocytes shown in (A). All data points are the mean ± standard error of the mean of six independent samples, and values were expressed as a relative quantity (RQ) to the vehicle control. * p
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    R&D Systems enzyme linked immunosorbent assay elisa
    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.
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    EUROIMMUN enzyme linked immunosorbent assay elisa
    STROBE flowchart of participating population. <t>ELISA,</t> enzyme-linked <t>immunosorbent</t> assay; IIFT, indirect immunofluorescence test; MERS, Middle east respiratory syndrome.
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    Immunodiagnostic Systems enzyme linked immunosorbent assay elisa
    STROBE flowchart of participating population. <t>ELISA,</t> enzyme-linked <t>immunosorbent</t> assay; IIFT, indirect immunofluorescence test; MERS, Middle east respiratory syndrome.
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    ALPCO enzyme linked immunosorbent assay elisa
    Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .
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    Pharmingen enzyme linked immunosorbent assays elisa
    Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .
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    Calbiotech enzyme linked immunosorbent assay elisa
    Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .
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    MyBiosource enzyme linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    R&D Systems enzyme‑linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    American Diagnostica enzyme linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    Cambridge Bioscience enzyme linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    ZEUS Scientific enzyme linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    AESKU enzyme linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    Tecan Systems enzyme linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    Inova Diagnostics enzyme linked immunosorbent assay elisa
    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked <t>immunosorbent</t> assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P
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    Thermo Fisher enzyme linked immunosorbent assay elisa
    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA).</t> Data are shown as the mean ± s.e.m. of three independent experiments.
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    BioLegend enzyme linked immunosorbent assay elisa
    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P
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    BG Medicine enzyme linked immunosorbent assay elisa
    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P
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    Biomedica enzyme linked immunosorbent assay elisa
    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P
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    Dade Behring enzyme linked immunosorbent assay elisa
    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Dade Behring, used in various techniques. Bioz Stars score: 92/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked <t>immunosorbent</t> assay <t>(ELISA)</t> (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P
    Enzyme Linked Immunosorbent Assay Elisa, supplied by ZeptoMetrix, used in various techniques. Bioz Stars score: 93/100, based on 272 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effect of PMK-S005 on the levels of proinflammatory cytokines and COX-2 expression with aging. The mucosal levels of tumor necrosis factor α (TNF-α) (A) and interleukin 1β (IL-1β) (B) were determined using an enzyme-linked <t>immunosorbent</t> assay. COX-2 protein expression in the gastric mucosa was evaluated by Western blot analysis (C). A representative Western blot assay is shown (D). The results are expressed as the means±SEM of five animals per group. PMK5, 5 mg/kg of PMK-S005; PMK10, 10 mg/kg of PMK-S005; Lanso, 5 mg/kg of Lansoprazole. *p
    Enzyme Linked Immunosorbent Assay Elisa, supplied by Hycult Biotech, used in various techniques. Bioz Stars score: 94/100, based on 184 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Innogenetics enzyme linked immunosorbent assay elisa
    Effect of PMK-S005 on the levels of proinflammatory cytokines and COX-2 expression with aging. The mucosal levels of tumor necrosis factor α (TNF-α) (A) and interleukin 1β (IL-1β) (B) were determined using an enzyme-linked <t>immunosorbent</t> assay. COX-2 protein expression in the gastric mucosa was evaluated by Western blot analysis (C). A representative Western blot assay is shown (D). The results are expressed as the means±SEM of five animals per group. PMK5, 5 mg/kg of PMK-S005; PMK10, 10 mg/kg of PMK-S005; Lanso, 5 mg/kg of Lansoprazole. *p
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    Image Search Results


    MS-444, a small molecule inhibitor of human antigen R (HuR), attenuates cytokine messenger RNA expression in stretch-injured astrocytes. (A) Twenty-four hours after injury, RNA levels were measured by quantitative real-time polymerase chain reaction analysis. (B) Enzyme-linked immunosorbent assay analysis of conditioned media from astrocytes shown in (A). All data points are the mean ± standard error of the mean of six independent samples, and values were expressed as a relative quantity (RQ) to the vehicle control. * p

    Journal: Journal of Neurotrauma

    Article Title: RNA Binding Protein Human Antigen R Is Translocated in Astrocytes following Spinal Cord Injury and Promotes the Inflammatory Response

    doi: 10.1089/neu.2016.4757

    Figure Lengend Snippet: MS-444, a small molecule inhibitor of human antigen R (HuR), attenuates cytokine messenger RNA expression in stretch-injured astrocytes. (A) Twenty-four hours after injury, RNA levels were measured by quantitative real-time polymerase chain reaction analysis. (B) Enzyme-linked immunosorbent assay analysis of conditioned media from astrocytes shown in (A). All data points are the mean ± standard error of the mean of six independent samples, and values were expressed as a relative quantity (RQ) to the vehicle control. * p

    Article Snippet: Cytokine expression was analyzed using enzyme-linked immunosorbent assay (ELISA) for the following cytokines: TNF-α, IL-6, C-X-C motif chemokine ligand 1 (CXCL-1), leukemia inhibitory factor (LIF), and C-C motif ligand 2 (CCL2; R & D Biosystems, Minneapolis, MN), and IL-1β (Abcam, Cambridge, UK).

    Techniques: Mass Spectrometry, RNA Expression, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked immunosorbent assay (ELISA) in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.

    Journal: Thrombosis and haemostasis

    Article Title: Increased Neutrophil Activation and Plasma DNA Levels in Patients with Pre-Eclampsia

    doi: 10.1055/s-0038-1675788

    Figure Lengend Snippet: Plasma myeloperoxidase (MPO) and histone levels. MPO ( A ), histone ( B ) and histone H3 ( C ) levels were measured by enzyme-linked immunosorbent assay (ELISA) in plasma from non-pregnant (Non-P), normal pregnant (NP) and pre-eclampsia (PE) women. Sample numbers ( n ) in each group are indicated. Data were analysed by Kruskal–Wallis and Mann–Whitney tests with Bonferroni correction for multiple comparisons. ns, not statistically significant. Correlations between plasma deoxyribonucleic acid (DNA) levels and MPO ( D ) or histone ( E ) levels in normal pregnant and PE women were analysed by computing Pearson’s correlation coefficient.

    Article Snippet: Plasma MPO levels were measured by enzyme-linked immunosorbent assay (ELISA) (R & D Systems, DMYE00B).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    STROBE flowchart of participating population. ELISA, enzyme-linked immunosorbent assay; IIFT, indirect immunofluorescence test; MERS, Middle east respiratory syndrome.

    Journal: Clinical Microbiology and Infection

    Article Title: Surveillance of the Middle East respiratory syndrome (MERS) coronavirus (CoV) infection in healthcare workers after contact with confirmed MERS patients: incidence and risk factors of MERS-CoV seropositivity

    doi: 10.1016/j.cmi.2016.07.017

    Figure Lengend Snippet: STROBE flowchart of participating population. ELISA, enzyme-linked immunosorbent assay; IIFT, indirect immunofluorescence test; MERS, Middle east respiratory syndrome.

    Article Snippet: Laboratory procedures We performed an enzyme-linked immunosorbent assay (ELISA) (Euroimmun, Lübeck, Germany) to screen for the presence of MERS-CoV IgG.

    Techniques: Enzyme-linked Immunosorbent Assay, Immunofluorescence

    Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked immunosorbent assay (ELISA). Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .

    Journal: Clinical and Experimental Immunology

    Article Title: Vitamin D3 correlates inversely with systemic dendritic cell numbers and bone erosion in chronic rhinosinusitis with nasal polyps and allergic fungal rhinosinusitis

    doi: 10.1111/j.1365-2249.2011.04325.x

    Figure Lengend Snippet: Circulating levels of dendritic cell (DC) regulatory factors are up-regulated in chronic rhinosinusitis (CRS) correlated inversely with 25-OH vitamin D 3 (VD 3 ). Plasma levels of (a) granulocyte–macrophage colony-stimulating factor (GM-CSF) and (b) prostaglandin E 2 (PGE 2 ) as measured by enxyme-linked immunosorbent assay (ELISA). Statistics shown represent Student's t -test between indicated patient groups. Pearson's correlation analysis of plasma levels for GM-CSF (c) and PGE 2 (d) as determined by ELISA versus plasma 25-OH VD 3 .

    Article Snippet: Levels of 25-dihydroxy VD3 were measured by enzyme-linked immunosorbent assay (ELISA) (Alpco Immunoassays, Salem, NH, USA) according to the manufacturer's instructions.

    Techniques: Enzyme-linked Immunosorbent Assay

    PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked immunosorbent assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P

    Journal: Cell Death & Disease

    Article Title: PLOD3 promotes lung metastasis via regulation of STAT3

    doi: 10.1038/s41419-018-1186-5

    Figure Lengend Snippet: PLOD3-induced metastasis is independent of the RAS-MAPK pathway. a Ras expression assessed via western blot analysis following transfection with siPLOD3 in R-H460 cells (upper). Ras activity was determined via an enzyme-linked immunosorbent assay-based activity assay after siPLOD3 treatment in R-H460 cells (lower, x -axis: siCON, siPLOD3). b Protein levels of MAP kinases were determined via western blotting after siPLOD3 treatment in R-H460 cells. c A cell invasion assay showing that SB and SP have a limited effect on pro-metastatic PLOD3 in R-H460 cells. (upper). Protein levels of indicated proteins determined via western blotting after treatment with siPLOD3 and MAP kinase inhibitor (p38 inhibitor, SB203580 (SB); JNK inhibitor, SP600125 (SP)) in R-H460 cells (lower). Statistical significance was determined by Student’s t -test. *** P

    Article Snippet: Cells were incubated for 6 h and centrifuged at < centrifuge speed > for < duration > at < temperature > ; thereafter, the supernatants were collected for the PLOD3 secretion assay via western blotting (WB) and an enzyme-linked immunosorbent assay (ELISA) (MyBioSource, Cat No. MBS2516046).

    Techniques: Expressing, Western Blot, Transfection, Activity Assay, Enzyme-linked Immunosorbent Assay, Invasion Assay

    Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked immunosorbent assay (ELISA). Data are shown as the mean ± s.e.m. of three independent experiments.

    Journal: Clinical and Experimental Immunology

    Article Title: Natural killer cell activities of synbiotic Lactobacillus casei ssp. casei in conjunction with dextran

    doi: 10.1111/j.1365-2249.2005.02975.x

    Figure Lengend Snippet: Interleukin (IL)-12 production by human peripheral blood mononuclear cells (PBMC) co-cultured with Lactobacillus casei ssp. casei . The cells were co-cultured with the indicated doses of live L. casei ssp. casei, L. paracasei ssp. paracasei or L. acidophilus for 48 h. IL-12 production was analysed by enzyme-linked immunosorbent assay (ELISA). Data are shown as the mean ± s.e.m. of three independent experiments.

    Article Snippet: Culture supernatants were collected and analysed by enzyme-linked immunosorbent assay (ELISA) for secreted IL-12 ( e Bioscience, San Diego, CA, USA).

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked immunosorbent assay (ELISA) (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P

    Journal: Science Advances

    Article Title: NDR2 promotes the antiviral immune response via facilitating TRIM25-mediated RIG-I activation in macrophages

    doi: 10.1126/sciadv.aav0163

    Figure Lengend Snippet: NDR2 facilitates RNA virus–induced IFN-β, IL-6, and TNF-α production via a kinase activity–independent mechanism in macrophages. ( A and B ) IFN-β, IL-6, and TNF-α expression in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV in the indicated times was detected by enzyme-linked immunosorbent assay (ELISA) (A) and real-time polymerase chain reaction (PCR) analysis (B). ( C ) Real-time PCR analysis of IFN-β, IL-6, and TNF-α transcripts in NDR2 f/f and Lysm + NDR2 f/f PMs treated with phosphate-buffered saline (PBS) or with the infection of H1N1, SeV, RSV, EMCV, HSV-1, or MHV68 for 8 hours. ( D ) Fluorescence-activated cell sorting (FACS) analysis of enhanced green fluorescent protein (eGFP) fluorescence intensity in NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP. ( E ) VSV-eGFP titers by TCID 50 assay in supernatants of NDR2 f/f and Lysm + NDR2 f/f PMs infected with VSV-eGFP for 6 hours. ( F ) NDR2 f/f and Lysm + NDR2 f/f PMs were transfected with empty vector (mock), NDR2, or its kinase-inactive mutants overexpressing plasmids for 36 hours and then infected with VSV, followed by real-time PCR analysis of IFN-β, IL-6, and TNF-α expression. AA, K282A/T442A. ( G ) FACS analysis of RAW264.7 cells stably overexpressing NDR2 or its kinase-inactive mutants infected with VSV-eGFP. Data are means ± SD and are representative of three independent experiments. Student’s t test was used for statistical calculation. ns, no significance. * P

    Article Snippet: Enzyme-linked immunosorbent assay The protein levels of murine IFN-β, IL-6, and TNF-α in cell culture supernatants and sera were detected by enzyme-linked immunosorbent assay (ELISA) according to the manufacturers’ instructions (BioLegend and eBioscience).

    Techniques: Activity Assay, Expressing, Infection, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Polymerase Chain Reaction, Fluorescence, FACS, Transfection, Plasmid Preparation, Stable Transfection

    Effect of PMK-S005 on the levels of proinflammatory cytokines and COX-2 expression with aging. The mucosal levels of tumor necrosis factor α (TNF-α) (A) and interleukin 1β (IL-1β) (B) were determined using an enzyme-linked immunosorbent assay. COX-2 protein expression in the gastric mucosa was evaluated by Western blot analysis (C). A representative Western blot assay is shown (D). The results are expressed as the means±SEM of five animals per group. PMK5, 5 mg/kg of PMK-S005; PMK10, 10 mg/kg of PMK-S005; Lanso, 5 mg/kg of Lansoprazole. *p

    Journal: Gut and Liver

    Article Title: PMK-S005 Alleviates Age-Related Gastric Acid Secretion, Inflammation, and Oxidative Status in the Rat Stomach

    doi: 10.5009/gnl15584

    Figure Lengend Snippet: Effect of PMK-S005 on the levels of proinflammatory cytokines and COX-2 expression with aging. The mucosal levels of tumor necrosis factor α (TNF-α) (A) and interleukin 1β (IL-1β) (B) were determined using an enzyme-linked immunosorbent assay. COX-2 protein expression in the gastric mucosa was evaluated by Western blot analysis (C). A representative Western blot assay is shown (D). The results are expressed as the means±SEM of five animals per group. PMK5, 5 mg/kg of PMK-S005; PMK10, 10 mg/kg of PMK-S005; Lanso, 5 mg/kg of Lansoprazole. *p

    Article Snippet: Measurement of tumor necrosis factor α, and interleukin 1β An enzyme-linked immunosorbent assay (ELISA) was performed to measure the level of the cytokine expression using the appropriate kits from Hycult Biotechnology (Uden, The Netherlands) and R & D systems (Minneapolis, MN, USA) by following the manufacturer’s instructions.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Western Blot