eno1 Search Results


gapdh(3e12) monoclonal antibody  (Bioss)
94
Bioss gapdh(3e12) monoclonal antibody
Gapdh(3e12) Monoclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gapdh(3e12) monoclonal antibody/product/Bioss
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gapdh(3e12) monoclonal antibody - by Bioz Stars, 2025-01
94/100 stars
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gene exp eno1 hs00361415 m1  (Thermo Fisher)
90
Thermo Fisher gene exp eno1 hs00361415 m1
Gene Exp Eno1 Hs00361415 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp eno1 hs00361415 m1/product/Thermo Fisher
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gene exp eno1 hs00361415 m1 - by Bioz Stars, 2025-01
90/100 stars
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enolase  (Cell Signaling Technology Inc)
95
Cell Signaling Technology Inc enolase
Enolase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/enolase/product/Cell Signaling Technology Inc
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
enolase - by Bioz Stars, 2025-01
95/100 stars
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anti eno1  (Proteintech)
93
Proteintech anti eno1
(A) Venn diagram showing the number of shared and organ-specific RBPs identified. (B) Normalized signal sum of identified RBPs in ex vivo eRIC eluates (upper panel) and the corresponding input samples (middle panel) for each organ analyzed. Center lines indicate the median, box borders represent the interquartile range (IQR), and whiskers extend to ±1.5 time the IQR; outliers are shown as black dots (pairwise comparisons using t-test with FDR correction, ***p.adj < 2e-16; n.s.: not significant). Bottom panel: amount of poly(A) RNA isolated from each organ by eRIC. Note that the retrieved mass of protein (upper panel) differs extensively across the tissues analyzed (with kidney >> liver > brain) and does not necessarily correlate with the mass of RNA recovered (bottom panel) (see also Table S2). (C) Hierarchical clustering and heatmap of the RBPs identified in brain, kidney and liver, showing protein abundance in eRIC eluates (left columns) and inputs (right columns) across the three organs. (D) Representative images of the proximity ligation assay (PLA) for interactions of <t>ENO1</t> and poly(A) RNA, nuclear staining (DAPI) and ENO1 immunofluorescence in brain, kidney and liver. Scale bar, 20 µM. See quantification in Figure S2B.
Anti Eno1, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti eno1/product/Proteintech
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti eno1 - by Bioz Stars, 2025-01
93/100 stars
  Buy from Supplier
gene exp eno1 mm01619597 g1  (Thermo Fisher)
86
Thermo Fisher gene exp eno1 mm01619597 g1
(A) Venn diagram showing the number of shared and organ-specific RBPs identified. (B) Normalized signal sum of identified RBPs in ex vivo eRIC eluates (upper panel) and the corresponding input samples (middle panel) for each organ analyzed. Center lines indicate the median, box borders represent the interquartile range (IQR), and whiskers extend to ±1.5 time the IQR; outliers are shown as black dots (pairwise comparisons using t-test with FDR correction, ***p.adj < 2e-16; n.s.: not significant). Bottom panel: amount of poly(A) RNA isolated from each organ by eRIC. Note that the retrieved mass of protein (upper panel) differs extensively across the tissues analyzed (with kidney >> liver > brain) and does not necessarily correlate with the mass of RNA recovered (bottom panel) (see also Table S2). (C) Hierarchical clustering and heatmap of the RBPs identified in brain, kidney and liver, showing protein abundance in eRIC eluates (left columns) and inputs (right columns) across the three organs. (D) Representative images of the proximity ligation assay (PLA) for interactions of <t>ENO1</t> and poly(A) RNA, nuclear staining (DAPI) and ENO1 immunofluorescence in brain, kidney and liver. Scale bar, 20 µM. See quantification in Figure S2B.
Gene Exp Eno1 Mm01619597 G1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/gene exp eno1 mm01619597 g1/product/Thermo Fisher
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
gene exp eno1 mm01619597 g1 - by Bioz Stars, 2025-01
86/100 stars
  Buy from Supplier

Image Search Results


(A) Venn diagram showing the number of shared and organ-specific RBPs identified. (B) Normalized signal sum of identified RBPs in ex vivo eRIC eluates (upper panel) and the corresponding input samples (middle panel) for each organ analyzed. Center lines indicate the median, box borders represent the interquartile range (IQR), and whiskers extend to ±1.5 time the IQR; outliers are shown as black dots (pairwise comparisons using t-test with FDR correction, ***p.adj < 2e-16; n.s.: not significant). Bottom panel: amount of poly(A) RNA isolated from each organ by eRIC. Note that the retrieved mass of protein (upper panel) differs extensively across the tissues analyzed (with kidney >> liver > brain) and does not necessarily correlate with the mass of RNA recovered (bottom panel) (see also Table S2). (C) Hierarchical clustering and heatmap of the RBPs identified in brain, kidney and liver, showing protein abundance in eRIC eluates (left columns) and inputs (right columns) across the three organs. (D) Representative images of the proximity ligation assay (PLA) for interactions of ENO1 and poly(A) RNA, nuclear staining (DAPI) and ENO1 immunofluorescence in brain, kidney and liver. Scale bar, 20 µM. See quantification in Figure S2B.

Journal: bioRxiv

Article Title: The RNA-binding protein landscapes differ between mammalian organs and cultured cells

doi: 10.1101/2022.02.10.479897

Figure Lengend Snippet: (A) Venn diagram showing the number of shared and organ-specific RBPs identified. (B) Normalized signal sum of identified RBPs in ex vivo eRIC eluates (upper panel) and the corresponding input samples (middle panel) for each organ analyzed. Center lines indicate the median, box borders represent the interquartile range (IQR), and whiskers extend to ±1.5 time the IQR; outliers are shown as black dots (pairwise comparisons using t-test with FDR correction, ***p.adj < 2e-16; n.s.: not significant). Bottom panel: amount of poly(A) RNA isolated from each organ by eRIC. Note that the retrieved mass of protein (upper panel) differs extensively across the tissues analyzed (with kidney >> liver > brain) and does not necessarily correlate with the mass of RNA recovered (bottom panel) (see also Table S2). (C) Hierarchical clustering and heatmap of the RBPs identified in brain, kidney and liver, showing protein abundance in eRIC eluates (left columns) and inputs (right columns) across the three organs. (D) Representative images of the proximity ligation assay (PLA) for interactions of ENO1 and poly(A) RNA, nuclear staining (DAPI) and ENO1 immunofluorescence in brain, kidney and liver. Scale bar, 20 µM. See quantification in Figure S2B.

Article Snippet: Subsequently, the Duolink PLA Fluorescence protocol (Sigma) was followed using an anti-biotin antibody (mouse: 1:400; ab201341, Abcam) and either the anti-ENO1 (rabbit: 1:400; Proteintech, 11204-1-AP), anti-SLC3A2 (rabbit: 1:400; Santa Cruz Biotechnology, sc-9160), anti-DDX6 (rabbit: 1:400; Novus Biologicals, NB200-192) or anti-PKM1 antibody (rabbit: 1:400; Cell Signalling, D30G6) for detection of the protein–RNA signal.

Techniques: Ex Vivo, Isolation, Proximity Ligation Assay, Staining, Immunofluorescence