Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: ENO1 expression in liver cancer tissue and peripheral blood of patients with liver cancer. (A) ENO1 expression in liver cancer tissues (×100). (B) ENO1 was not expressed in benign liver lesions (×100). (C) Serum anti-ENO1 antibody levels in patients with liver cancer were higher than those in patients with benign liver lesions and healthy controls. (D) ENO1 expression in liver cancer tissues (×400). (E) ENO1 was not expressed in benign liver lesion (×400). (F) Receiver operating characteristic curve analysis for liver cancer diagnosis using anti-ENO1 antibody levels. The AUC was 0.741, the sensitivity was 64.3% and the specificity was 85.5%. The red dot represents the cut-off value. **P<0.01; ****P<0.0001 ENO1, α-enolase; AUC, area under the curve.

Article Snippet: The anti-ENO1 antibody ELISA detection reagent kit (cat. no. CSB-EQ027775HU) was purchased from CUSABIO Technology LLC.

Techniques: Expressing

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: ENO1 expression in pathological tissues.

Article Snippet: The anti-ENO1 antibody ELISA detection reagent kit (cat. no. CSB-EQ027775HU) was purchased from CUSABIO Technology LLC.

Techniques: Expressing

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Comparison of the serum anti-ENO1 antibody levels among the three groups of participants [P50 (P25-P75)].

Article Snippet: The anti-ENO1 antibody ELISA detection reagent kit (cat. no. CSB-EQ027775HU) was purchased from CUSABIO Technology LLC.

Techniques:

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Validation of the siRNA interference effect on ENO1 expression in liver cancer cells. (A) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (B) RT-qPCR validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 gene in the si-1 and si-2 groups were lower than those in the control group. (C) Western blot validation of the effect of siRNA interference on ENO1 expression in HepG2 cells. The results showed that the relative expression levels of the ENO1 protein in HepG2 cells in the si-1 and si-2 groups were significantly lower than those in the control group. (D) Western blot validation of the effect of siRNA interference on ENO1 expression in Huh7 cells. The results showed that the relative expression levels of the ENO1 protein in Huh7 cells in the si-1 and si-2 groups were significantly lower than those in the control group. **P<0.01; ****P<0.0001. siRNA, small interfering RNA; ENO1, α-enolase; si-1, siRNA interference group 1; si-2, siRNA interference group 2; RT-qPCR, reverse transcription-quantitative PCR.

Article Snippet: The anti-ENO1 antibody ELISA detection reagent kit (cat. no. CSB-EQ027775HU) was purchased from CUSABIO Technology LLC.

Techniques: Expressing, Quantitative RT-PCR, Western Blot, Small Interfering RNA, Real-time Polymerase Chain Reaction

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Effect of ENO1 siRNA on liver cancer cell proliferation. (A) HepG2 and (B) Huh7 cell proliferation was suppressed after ENO1 siRNA treatment. Results show that 72 h after transfection in the si-1 and si-2 groups, ENO1 siRNA treatment resulted in proliferation inhibition in HepG2 and Huh7 cells. In HepG2 cells, the differences in cell proliferation between the NC and the siRNA-treated groups were statistically significant (P<0.05 vs. si-2 group and P<0.01 vs. si-1 group). Cell proliferation in Huh7 cells was also significantly different. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; si-1, siRNA interference group 1; si-2, siRNA interference group 2; OD, optical density; NC, negative control.

Article Snippet: The anti-ENO1 antibody ELISA detection reagent kit (cat. no. CSB-EQ027775HU) was purchased from CUSABIO Technology LLC.

Techniques: Transfection, Inhibition, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Effect of ENO1 siRNA on the migration ability of liver cancer cells. (A) Compared with that of the NC group, HepG2 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (B) Gap size was measured using ImageJ software and the ratio of gap size after ENO1 siRNA treatment in HepG2 cells was calculated based on the size of the wound at 0 h. (C) Compared with that of the NC group, Huh7 cell migration after ENO1 siRNA treatment became slower in the si-1 and si-2 groups, and the difference was statistically significant at 48 h after treatment. (D) Gap size was measured with the ImageJ software and the ratio of gap size after ENO1 siRNA treatment in Huh7 cell was calculated based on the size of the wound at 0 h. *P<0.05; **P<0.01. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

Article Snippet: The anti-ENO1 antibody ELISA detection reagent kit (cat. no. CSB-EQ027775HU) was purchased from CUSABIO Technology LLC.

Techniques: Migration, Software, Small Interfering RNA, Negative Control

Journal: Oncology Letters

Article Title: α-enolase is highly expressed in liver cancer and promotes cancer cell invasion and metastasis

doi: 10.3892/ol.2020.12003

Figure Lengend Snippet: Effect of ENO1 siRNA on the invasion and migration abilities of liver cancer cells. Compared with the NC group, the in vitro invasion and migration abilities of (A) HepG2 and (B) Huh7 cells after ENO1 siRNA treatment decreased in the si-1 and si-2 groups, and the differences were statistically significant. Numbers of (C) HepG2 and (D) Huh7 cells were measured with the ImageJ software after ENO1 siRNA treatment. *P<0.05; **P<0.01; ***P<0.001; ****P<0.0001. ENO1, α-enolase; siRNA, small interfering RNA; NC, negative control; si-1, siRNA interference group 1; si-2, siRNA interference group 2.

Article Snippet: The anti-ENO1 antibody ELISA detection reagent kit (cat. no. CSB-EQ027775HU) was purchased from CUSABIO Technology LLC.

Techniques: Migration, In Vitro, Software, Small Interfering RNA, Negative Control

Journal:

Article Title: Microarray and Real-Time PCR Analysis of Adrenal Gland Gene Expression in the 7-day-old Rat: Effects of Hypoxia from Birth

doi: 10.1152/physiolgenomics.00245.2006

Figure Lengend Snippet: TaqMan Gene Expression Assays Used for Real-Time PCR

Article Snippet: Differences in gene expression, expressed as fold-change, were calculated using the 2 −ΔΔCt method using 18S as the internal control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Assay ID# Cytochrome P450, subfamily 1B, polypeptide 1 Cyp1b1 Rn00564055_m1 Guanosine monophosphate reductase Gmpr Rn00589361_m1 Xanthine dehydrogenase Xdh Rn00567654_m1 Cadherin 1 Cdh1 Rn00580109_m1 Phospholipase A2, group IVA (cytosolic, Ca-dependent) Pla2g4a Rn00591916_m1 Aldehyde oxidase Aox1 Rn00571242_m1 Regulator of G-protein signaling protein 2 Rgs2 Rn00584932_m1 Carnitine palmitoyltransferase 2 Cpt2 Rn00563995_m1 Steroid 5 alpha-reductase 1 Srd5a1 Rn00567064_m1 Enolase 1, alpha Eno1 Rn00820594_g1 N-ethylmaleimide sensitive factor Nsf Rn00572694_m1 Cytochrome P450, subfamily 11B, polypeptide 3 Cyp11b3 Rn00822066_g1 Steroidogenic acute regulatory protein StAR Rn00580695_m1 Cytochrome P450, subfamily 11A Cyp11a Rn00568733_m1 Cytochrome P450, subfamily 21A, polypeptide 1 Cyp21a1 Rn00588996_g1 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Hmgcr Rn00565598_m1 Low density lipoprotein receptor Ldlr Rn00598438_m1 Vascular endothelial growth factor Vegf Rn00582935_m1 Hypoxia-inducible factor 1 alpha Hif1a Rn00577560_m1 Open in a separate window Each Gene Expression Assay consisted of a mix of sequence specific forward/reverse primers and a FAM-labeled probe.

Techniques: Expressing

Journal:

Article Title: Microarray and Real-Time PCR Analysis of Adrenal Gland Gene Expression in the 7-day-old Rat: Effects of Hypoxia from Birth

doi: 10.1152/physiolgenomics.00245.2006

Figure Lengend Snippet: Microarray analysis of adrenal gene expression in the 7-day-old rat: effects of hypoxia from birth.

Article Snippet: Differences in gene expression, expressed as fold-change, were calculated using the 2 −ΔΔCt method using 18S as the internal control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Assay ID# Cytochrome P450, subfamily 1B, polypeptide 1 Cyp1b1 Rn00564055_m1 Guanosine monophosphate reductase Gmpr Rn00589361_m1 Xanthine dehydrogenase Xdh Rn00567654_m1 Cadherin 1 Cdh1 Rn00580109_m1 Phospholipase A2, group IVA (cytosolic, Ca-dependent) Pla2g4a Rn00591916_m1 Aldehyde oxidase Aox1 Rn00571242_m1 Regulator of G-protein signaling protein 2 Rgs2 Rn00584932_m1 Carnitine palmitoyltransferase 2 Cpt2 Rn00563995_m1 Steroid 5 alpha-reductase 1 Srd5a1 Rn00567064_m1 Enolase 1, alpha Eno1 Rn00820594_g1 N-ethylmaleimide sensitive factor Nsf Rn00572694_m1 Cytochrome P450, subfamily 11B, polypeptide 3 Cyp11b3 Rn00822066_g1 Steroidogenic acute regulatory protein StAR Rn00580695_m1 Cytochrome P450, subfamily 11A Cyp11a Rn00568733_m1 Cytochrome P450, subfamily 21A, polypeptide 1 Cyp21a1 Rn00588996_g1 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Hmgcr Rn00565598_m1 Low density lipoprotein receptor Ldlr Rn00598438_m1 Vascular endothelial growth factor Vegf Rn00582935_m1 Hypoxia-inducible factor 1 alpha Hif1a Rn00577560_m1 Open in a separate window Each Gene Expression Assay consisted of a mix of sequence specific forward/reverse primers and a FAM-labeled probe.

Techniques: Microarray, Expressing, Migration, Activity Assay

Journal:

Article Title: Microarray and Real-Time PCR Analysis of Adrenal Gland Gene Expression in the 7-day-old Rat: Effects of Hypoxia from Birth

doi: 10.1152/physiolgenomics.00245.2006

Figure Lengend Snippet: Real-time PCR analysis of adrenal gene expression: verification of selected microarray results.

Article Snippet: Differences in gene expression, expressed as fold-change, were calculated using the 2 −ΔΔCt method using 18S as the internal control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Assay ID# Cytochrome P450, subfamily 1B, polypeptide 1 Cyp1b1 Rn00564055_m1 Guanosine monophosphate reductase Gmpr Rn00589361_m1 Xanthine dehydrogenase Xdh Rn00567654_m1 Cadherin 1 Cdh1 Rn00580109_m1 Phospholipase A2, group IVA (cytosolic, Ca-dependent) Pla2g4a Rn00591916_m1 Aldehyde oxidase Aox1 Rn00571242_m1 Regulator of G-protein signaling protein 2 Rgs2 Rn00584932_m1 Carnitine palmitoyltransferase 2 Cpt2 Rn00563995_m1 Steroid 5 alpha-reductase 1 Srd5a1 Rn00567064_m1 Enolase 1, alpha Eno1 Rn00820594_g1 N-ethylmaleimide sensitive factor Nsf Rn00572694_m1 Cytochrome P450, subfamily 11B, polypeptide 3 Cyp11b3 Rn00822066_g1 Steroidogenic acute regulatory protein StAR Rn00580695_m1 Cytochrome P450, subfamily 11A Cyp11a Rn00568733_m1 Cytochrome P450, subfamily 21A, polypeptide 1 Cyp21a1 Rn00588996_g1 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Hmgcr Rn00565598_m1 Low density lipoprotein receptor Ldlr Rn00598438_m1 Vascular endothelial growth factor Vegf Rn00582935_m1 Hypoxia-inducible factor 1 alpha Hif1a Rn00577560_m1 Open in a separate window Each Gene Expression Assay consisted of a mix of sequence specific forward/reverse primers and a FAM-labeled probe.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Microarray

Journal:

Article Title: Microarray and Real-Time PCR Analysis of Adrenal Gland Gene Expression in the 7-day-old Rat: Effects of Hypoxia from Birth

doi: 10.1152/physiolgenomics.00245.2006

Figure Lengend Snippet: Real-time PCR analysis of adrenal gene expression: analysis of genes not meeting microarray cutoff criteria.

Article Snippet: Differences in gene expression, expressed as fold-change, were calculated using the 2 −ΔΔCt method using 18S as the internal control. table ft1 table-wrap mode="anchored" t5 caption a7 Gene Symbol Assay ID# Cytochrome P450, subfamily 1B, polypeptide 1 Cyp1b1 Rn00564055_m1 Guanosine monophosphate reductase Gmpr Rn00589361_m1 Xanthine dehydrogenase Xdh Rn00567654_m1 Cadherin 1 Cdh1 Rn00580109_m1 Phospholipase A2, group IVA (cytosolic, Ca-dependent) Pla2g4a Rn00591916_m1 Aldehyde oxidase Aox1 Rn00571242_m1 Regulator of G-protein signaling protein 2 Rgs2 Rn00584932_m1 Carnitine palmitoyltransferase 2 Cpt2 Rn00563995_m1 Steroid 5 alpha-reductase 1 Srd5a1 Rn00567064_m1 Enolase 1, alpha Eno1 Rn00820594_g1 N-ethylmaleimide sensitive factor Nsf Rn00572694_m1 Cytochrome P450, subfamily 11B, polypeptide 3 Cyp11b3 Rn00822066_g1 Steroidogenic acute regulatory protein StAR Rn00580695_m1 Cytochrome P450, subfamily 11A Cyp11a Rn00568733_m1 Cytochrome P450, subfamily 21A, polypeptide 1 Cyp21a1 Rn00588996_g1 3-hydroxy-3-methylglutaryl-Coenzyme A reductase Hmgcr Rn00565598_m1 Low density lipoprotein receptor Ldlr Rn00598438_m1 Vascular endothelial growth factor Vegf Rn00582935_m1 Hypoxia-inducible factor 1 alpha Hif1a Rn00577560_m1 Open in a separate window Each Gene Expression Assay consisted of a mix of sequence specific forward/reverse primers and a FAM-labeled probe.

Techniques: Real-time Polymerase Chain Reaction, Expressing, Microarray

Journal: Metabolomics

Article Title: Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12)

doi: 10.1007/s11306-017-1236-5

Figure Lengend Snippet: List of genes measured by real-time RT-PCR in this study. TaqMan ® gene expression assays. The following TaqMan probes (Thermo Fisher Scientific; Waltham, MA, USA) were used in our study

Article Snippet: Dlst (Mm00513470_m1) , Eno1 (Mm01619597_g1) , Fh1 (Mm01321349_m1).

Techniques: Quantitative RT-PCR, Expressing

Journal: Metabolomics

Article Title: Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12)

doi: 10.1007/s11306-017-1236-5

Figure Lengend Snippet: Metabolic profiling of intracellular glycometabolism. Changes in metabolite levels after 48 h exposure to everolimus ( closed bars , EVE) were compared with those without everolimus at 48 h (Control). The data represent the mean ± S.D. (n = 3). Statistically significant differences between the EVE and control: *p < 0.05; **p < 0.01 (Student’s t -test). Abbreviations: G6P glucose 6-phosphate, F6P fructose 6-phosphate, F1,6P fructose 1,6-bisphosphate, GAP glyceraldehyde phosphate, DHAP dihydroxyacetone phosphate, 1,3-Bis-PG 1,3-Bis phosphoglycerate, 3PG 3-phosphoglycerate, 2PG 2-phosphoglycerate, PEP phosphoenolpyruvate, Ac-CoA acetyl CoA, Ru5P ribulose 5-phosphate, R5P ribose 5-phosphate, Xu5P xylulose 5-phosphate, mTOR mammalian target of rapamycin, HIF1a hypoxia-inducible factor-1α, GLUT glucose transporter, HK1 hexokinase-1, GPI glucose-6-phosphate isomerase, PFKM 6-phosphofructokinase, ALDOA aldolase, TPI1P2 triosephosphate isomerase, GAPDH glyceraldehyde 3-phosphate dehydrogenase, PGK1 phosphoglycerate kinase-1, ENO1 enolase-1, PKM2 pyruvate kinase-2, LDHA _lactate dehydrogenase A, PDHA1 pyruvate dehydrogenase α1, G6PD 6-phosphate dehydrogenase, PGD 6-phosphogluconate dehydrogenase, RPIA ribose-5-phosphate isomerase A, TKT transketolase, TALDO1 transaldolase-1, CS citrate synthase, ACO1 aconitase-1, IDH1 isocitrate dehydrogenase-1, OGDH α-ketoglutarate dehydrogenase, DLST dihydrolipoamide succinyltransferase, SUCLG2 succinyl-CoA ligase, SDHA succinate dehydrogenase A, FH fumarate hydratase, MDH1 malate dehydrogenase-1

Article Snippet: Dlst (Mm00513470_m1) , Eno1 (Mm01619597_g1) , Fh1 (Mm01321349_m1).

Techniques:

Journal: Diagnostics

Article Title: Overexpressed Proteins in HCC Cell-Derived Exosomes, CCT8, and Cofilin-1 Are Potential Biomarkers for Patients with HCC

doi: 10.3390/diagnostics11071221

Figure Lengend Snippet: Diagnostic efficiency of annexin V, CCT8, CFL1, ENO1, HSPB1, and TPM4 in diagnosing HCC in the test cohort comprised of normal healthy individuals (normal) and patients with HCC. ( a ) Comparison of the six protein expressions between 14 healthy individuals (normal) and 15 patients with HCC. ( b ) Area under the curve (AUC) and receiver operating characteristics (ROC) of six protein markers in diagnosing HCC. ( c ) Left panel: relative expression of CCT8 and CFL1 in serum exosomal mRNA of 29 healthy individuals (normal) and 20 patients with HCC. Right panel: area under the curve (AUC) and receiver operating characteristics (ROC) of serum exosomal CCT8 and CFL1 expression.

Article Snippet: The following commercially available ELISA kits were used: enolase 1 (ENO1) (CSB-E17177h; Cusabio, Houston, TX, USA), CCT8 (MBS2516261), CFL1 (MBS2505977), ANXA5 (MBS704883), HSPB1 (MBS727021), and TPM4 (MBS944560; all previously named kits from Mybiosource Inc., San Diego, CA, USA).

Techniques: Diagnostic Assay, Expressing