elisa-based assays Search Results


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  • 90
    Thermo Fisher monoclonal antibody based elisa kits
    Monoclonal Antibody Based Elisa Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Millipore elisa based assay
    Elisa Based Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Pepscan pepscan based elisa
    CR57 recognizes a linear epitope on rabies virus glycoprotein. (A) CR57 was tested using an 8-mer peptide <t>PEPSCAN-ELISA</t> in a region of the rabies virus glycoprotein ectodomain identified using overlapping 15-mer peptides. (B) Alanine replacement scan through the core 8-mer peptide containing the minimal binding region as identified in panel A. Relative ELISA signals are shown for each peptide.
    Pepscan Based Elisa, supplied by Pepscan, used in various techniques. Bioz Stars score: 88/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cayman Chemical elisa based detection kit
    CR57 recognizes a linear epitope on rabies virus glycoprotein. (A) CR57 was tested using an 8-mer peptide <t>PEPSCAN-ELISA</t> in a region of the rabies virus glycoprotein ectodomain identified using overlapping 15-mer peptides. (B) Alanine replacement scan through the core 8-mer peptide containing the minimal binding region as identified in panel A. Relative ELISA signals are shown for each peptide.
    Elisa Based Detection Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 88/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    NewEast Biosciences monoclonal anti camp antibody based direct camp elisa kit
    <t>cAMP</t> <t>ELISA</t>
    Monoclonal Anti Camp Antibody Based Direct Camp Elisa Kit, supplied by NewEast Biosciences, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher elisa based assay
    <t>cAMP</t> <t>ELISA</t>
    Elisa Based Assay, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Bio-Rad multiplex biometric elisa based immunoassay
    <t>cAMP</t> <t>ELISA</t>
    Multiplex Biometric Elisa Based Immunoassay, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 88/100, based on 136 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    RayBiotech cell based elisa kit
    Signal transducer and activator of transcription-1 <t>(STAT1)</t> phosphorylation induced by IFN-γ in PrEC, PC-3, DU-145, and LNCaP cells. The STAT1 phosphorylation was determined by cell-based <t>ELISA</t> technique. Each point represents the mean of cellular
    Cell Based Elisa Kit, supplied by RayBiotech, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Cytoskeleton Inc elisa based assay
    CD98hc mediates adhesion-induced <t>RhoA</t> activation and matrix contraction to enable Fn matrix assembly. (A) RhoA activity was measured in an <t>ELISA-based</t> Rho assay in WT and CD98hc-deficient cells after plating on a 3D Fn matrix. The error bars represent SEM. The assay was repeated three times with similar results. Samples were also resolved by SDS-PAGE and immunoblotted with anti-RhoA antibody (total RhoA) to confirm that both WT and CD98hc-null MEFs express similar amounts of total RhoA (not depicted). (B) Activation of RhoA by LPA in WT and CD98hc-null MEFs. Adherent serum-starved CD98hc-null cells were treated with 1 μg/ml LPA or buffer, and RhoA activity was measured after 5 min. Values represent the mean and range of duplicate determinations. The assay was repeated twice with similar results. (C) Activation of RhoA bypasses the defect in matrix contraction in CD98hc-null cells. Clot contraction was measured 1 h after WT, CD98hc-null, and CD98hc-null MEFs were stimulated with LPA (see Materials and methods for details). Values represent the mean ± SEM of triplicate determinations. Depicted is one of two such experiments with identical results. (D) Activation of RhoA bypasses the defect in Fn matrix assembly in CD98hc-null cells. DOC-insoluble Fn produced by WT, CD98hc-null, and CD98hc-null MEFs treated with LPA was evaluated biochemically as described in Fig. 3 . CD98hc-null MEFs stimulated with LPA were able to assemble Fn into fibrils as efficiently as WT cells. Depicted are the means of triplicate measurements. The assay was repeated two times with similar results.
    Elisa Based Assay, supplied by Cytoskeleton Inc, used in various techniques. Bioz Stars score: 91/100, based on 67 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Indoor Biotechnologies monoclonal antibody based enzyme linked immunosorbent assay
    CD98hc mediates adhesion-induced <t>RhoA</t> activation and matrix contraction to enable Fn matrix assembly. (A) RhoA activity was measured in an <t>ELISA-based</t> Rho assay in WT and CD98hc-deficient cells after plating on a 3D Fn matrix. The error bars represent SEM. The assay was repeated three times with similar results. Samples were also resolved by SDS-PAGE and immunoblotted with anti-RhoA antibody (total RhoA) to confirm that both WT and CD98hc-null MEFs express similar amounts of total RhoA (not depicted). (B) Activation of RhoA by LPA in WT and CD98hc-null MEFs. Adherent serum-starved CD98hc-null cells were treated with 1 μg/ml LPA or buffer, and RhoA activity was measured after 5 min. Values represent the mean and range of duplicate determinations. The assay was repeated twice with similar results. (C) Activation of RhoA bypasses the defect in matrix contraction in CD98hc-null cells. Clot contraction was measured 1 h after WT, CD98hc-null, and CD98hc-null MEFs were stimulated with LPA (see Materials and methods for details). Values represent the mean ± SEM of triplicate determinations. Depicted is one of two such experiments with identical results. (D) Activation of RhoA bypasses the defect in Fn matrix assembly in CD98hc-null cells. DOC-insoluble Fn produced by WT, CD98hc-null, and CD98hc-null MEFs treated with LPA was evaluated biochemically as described in Fig. 3 . CD98hc-null MEFs stimulated with LPA were able to assemble Fn into fibrils as efficiently as WT cells. Depicted are the means of triplicate measurements. The assay was repeated two times with similar results.
    Monoclonal Antibody Based Enzyme Linked Immunosorbent Assay, supplied by Indoor Biotechnologies, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Quansys Biosciences elisa based array
    Altered intestinal microbiota augments pulmonary cytokine response to Sendai virus (SeV), but does not impair viral clearance. Mice were placed on reverse osmosis (RO) or RO + streptomycin water, and 2 weeks later inoculated with SeV. (A) RT polymerase chain reaction (PCR) quantification of SeV copy number in whole lungs at the indicated days post inoculation (PI) SeV ( n = 3–6 mice/day and group). (B) Expression of cytokines in the bronchoalveolar lavage fluid of mice 8 days PI SeV. Data are array results from a commercially available <t>ELISA</t> protein array (data combined from five separate experiments with n ≥ 2/group/experiment, with samples run in duplicate); note increased IL-6, IFNγ, and <t>CCL2</t> (MCP-1) protein. (C) Message for Il6 and Ifnγ , but not Ccl2 , was increased in whole lung of SeV-infected mice that had received streptomycin. Specific cytokine mRNA levels were assessed by RT PCR in whole of lung of mice at day 8 PI SeV or UV inactivated SeV ( n = 3/group and treatment) and normalized to Gapdh copy number.
    Elisa Based Array, supplied by Quansys Biosciences, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cayman Chemical elisa based assay
    Altered intestinal microbiota augments pulmonary cytokine response to Sendai virus (SeV), but does not impair viral clearance. Mice were placed on reverse osmosis (RO) or RO + streptomycin water, and 2 weeks later inoculated with SeV. (A) RT polymerase chain reaction (PCR) quantification of SeV copy number in whole lungs at the indicated days post inoculation (PI) SeV ( n = 3–6 mice/day and group). (B) Expression of cytokines in the bronchoalveolar lavage fluid of mice 8 days PI SeV. Data are array results from a commercially available <t>ELISA</t> protein array (data combined from five separate experiments with n ≥ 2/group/experiment, with samples run in duplicate); note increased IL-6, IFNγ, and <t>CCL2</t> (MCP-1) protein. (C) Message for Il6 and Ifnγ , but not Ccl2 , was increased in whole lung of SeV-infected mice that had received streptomycin. Specific cytokine mRNA levels were assessed by RT PCR in whole of lung of mice at day 8 PI SeV or UV inactivated SeV ( n = 3/group and treatment) and normalized to Gapdh copy number.
    Elisa Based Assay, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 91/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cayman Chemical elisa based dna binding assay kit
    ATG7 knockdown prevents RelA/p65 nuclear translocation and <t>DNA</t> binding, but not IκBα degradation or RelA/p65 phosphorylation. ( A ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and then treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and processed according to an <t>ELISA-based</t> DNA binding assay kit as described in the “ Materials and Methods ” section. Error bars represent mean ± S.E. (n = 4 for each condition). ( B C ) HPAEC were transfected with si-Con or si-ATG7 for 48 h and treated with thrombin (5 U/ml) for 1 h. Total cell lysates were analyzed by Western blot for ( B ) IκBα levels and ( C ) RelA/p65 phosphorylation. RelA/p65 was used as a loading control. Error bars represent mean ± S.E. (n = 3–4 for each condition). ( D ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and analyzed by Western blot to measure the level of RelA/p65, and TATA-binding protein (TBP) was used as a loading control for nuclear protein. Error bars represent mean ± S.E. (n = 3–4 for each condition).
    Elisa Based Dna Binding Assay Kit, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher elisa based kits
    ATG7 knockdown prevents RelA/p65 nuclear translocation and <t>DNA</t> binding, but not IκBα degradation or RelA/p65 phosphorylation. ( A ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and then treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and processed according to an <t>ELISA-based</t> DNA binding assay kit as described in the “ Materials and Methods ” section. Error bars represent mean ± S.E. (n = 4 for each condition). ( B C ) HPAEC were transfected with si-Con or si-ATG7 for 48 h and treated with thrombin (5 U/ml) for 1 h. Total cell lysates were analyzed by Western blot for ( B ) IκBα levels and ( C ) RelA/p65 phosphorylation. RelA/p65 was used as a loading control. Error bars represent mean ± S.E. (n = 3–4 for each condition). ( D ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and analyzed by Western blot to measure the level of RelA/p65, and TATA-binding protein (TBP) was used as a loading control for nuclear protein. Error bars represent mean ± S.E. (n = 3–4 for each condition).
    Elisa Based Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    diaDexus microplate based elisa
    ATG7 knockdown prevents RelA/p65 nuclear translocation and <t>DNA</t> binding, but not IκBα degradation or RelA/p65 phosphorylation. ( A ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and then treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and processed according to an <t>ELISA-based</t> DNA binding assay kit as described in the “ Materials and Methods ” section. Error bars represent mean ± S.E. (n = 4 for each condition). ( B C ) HPAEC were transfected with si-Con or si-ATG7 for 48 h and treated with thrombin (5 U/ml) for 1 h. Total cell lysates were analyzed by Western blot for ( B ) IκBα levels and ( C ) RelA/p65 phosphorylation. RelA/p65 was used as a loading control. Error bars represent mean ± S.E. (n = 3–4 for each condition). ( D ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and analyzed by Western blot to measure the level of RelA/p65, and TATA-binding protein (TBP) was used as a loading control for nuclear protein. Error bars represent mean ± S.E. (n = 3–4 for each condition).
    Microplate Based Elisa, supplied by diaDexus, used in various techniques. Bioz Stars score: 89/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad bead based multiple target sandwich elisa system
    Dynamic changes in whole-lung cytokines and chemokines were apparent in TLR9 +/+ and TLR9 −/− mice during invasive aspergillosis induced by resting and swollen conidia. (A to C) <t>ELISA</t> or <t>Bioplex</t> analysis of TNF-α
    Bead Based Multiple Target Sandwich Elisa System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bender MedSystems cytofluorimetry based elisa system
    Dynamic changes in whole-lung cytokines and chemokines were apparent in TLR9 +/+ and TLR9 −/− mice during invasive aspergillosis induced by resting and swollen conidia. (A to C) <t>ELISA</t> or <t>Bioplex</t> analysis of TNF-α
    Cytofluorimetry Based Elisa System, supplied by Bender MedSystems, used in various techniques. Bioz Stars score: 85/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Becton Dickinson elisa based assay
    (A) Release of cytokines and sTREM-1 from cultured monocytes. For cell activation, primary monocytes were cultured in 24-well flat-bottom tissue culture plates in the presence of LPS (1 μg/ml). In some experiments, this stimulus was provided in combination with <t>LP17</t> (10–100 ng/ml), control peptide (10–100 ng/ml), or rIL-10 (500 U/ml). To activate monocytes through TREM-1, an agonist anti–TREM-1 mAb (10 μg/ml) was added as indicated. Cell-free supernatants were analyzed for production of TNF-α, IL-1β, and sTREM-1 by <t>ELISA</t> or immunodot. All experiments were performed in triplicate and data are expressed as means (SEM). (a) Media; (b) LP17 10 ng/ml; (c) anti–TREM-1; (d) LPS; (e) LPS + anti–TREM-1; (f) LPS + LP17 10 ng/ml; (g) LPS + LP17 50 ng/ml; (h) LPS + LP17 100 ng/ml; (i) LPS + IL10. (B) Effect of LP17 on NF-κB activation. Monocytes were cultured for 24 h in the presence of E. coli LPS (O111:B4; 1 μg/ml), anti–TREM-1 mAb (10 μg/ml), and/or LP17 (100 ng/ml) as indicated, and the levels of NF-κB p50 and p65 were determined using an ELISA-based assay. Experiments were performed in triplicate, and data are expressed as means of optical densities (SEM).
    Elisa Based Assay, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Echelon Biosciences elisa based assay
    (A) Release of cytokines and sTREM-1 from cultured monocytes. For cell activation, primary monocytes were cultured in 24-well flat-bottom tissue culture plates in the presence of LPS (1 μg/ml). In some experiments, this stimulus was provided in combination with <t>LP17</t> (10–100 ng/ml), control peptide (10–100 ng/ml), or rIL-10 (500 U/ml). To activate monocytes through TREM-1, an agonist anti–TREM-1 mAb (10 μg/ml) was added as indicated. Cell-free supernatants were analyzed for production of TNF-α, IL-1β, and sTREM-1 by <t>ELISA</t> or immunodot. All experiments were performed in triplicate and data are expressed as means (SEM). (a) Media; (b) LP17 10 ng/ml; (c) anti–TREM-1; (d) LPS; (e) LPS + anti–TREM-1; (f) LPS + LP17 10 ng/ml; (g) LPS + LP17 50 ng/ml; (h) LPS + LP17 100 ng/ml; (i) LPS + IL10. (B) Effect of LP17 on NF-κB activation. Monocytes were cultured for 24 h in the presence of E. coli LPS (O111:B4; 1 μg/ml), anti–TREM-1 mAb (10 μg/ml), and/or LP17 (100 ng/ml) as indicated, and the levels of NF-κB p50 and p65 were determined using an ELISA-based assay. Experiments were performed in triplicate, and data are expressed as means of optical densities (SEM).
    Elisa Based Assay, supplied by Echelon Biosciences, used in various techniques. Bioz Stars score: 91/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cellestis elisa based quantiferon tb gold
    (A) Release of cytokines and sTREM-1 from cultured monocytes. For cell activation, primary monocytes were cultured in 24-well flat-bottom tissue culture plates in the presence of LPS (1 μg/ml). In some experiments, this stimulus was provided in combination with <t>LP17</t> (10–100 ng/ml), control peptide (10–100 ng/ml), or rIL-10 (500 U/ml). To activate monocytes through TREM-1, an agonist anti–TREM-1 mAb (10 μg/ml) was added as indicated. Cell-free supernatants were analyzed for production of TNF-α, IL-1β, and sTREM-1 by <t>ELISA</t> or immunodot. All experiments were performed in triplicate and data are expressed as means (SEM). (a) Media; (b) LP17 10 ng/ml; (c) anti–TREM-1; (d) LPS; (e) LPS + anti–TREM-1; (f) LPS + LP17 10 ng/ml; (g) LPS + LP17 50 ng/ml; (h) LPS + LP17 100 ng/ml; (i) LPS + IL10. (B) Effect of LP17 on NF-κB activation. Monocytes were cultured for 24 h in the presence of E. coli LPS (O111:B4; 1 μg/ml), anti–TREM-1 mAb (10 μg/ml), and/or LP17 (100 ng/ml) as indicated, and the levels of NF-κB p50 and p65 were determined using an ELISA-based assay. Experiments were performed in triplicate, and data are expressed as means of optical densities (SEM).
    Elisa Based Quantiferon Tb Gold, supplied by Cellestis, used in various techniques. Bioz Stars score: 85/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Meso Scale Diagnostics LLC elisa based meso scale discovery msd
    Specificity of human mutant and mouse HTT detection. The adeno-associated AAV-shRNA expression vector AAV-SEWB-sh4 was transduced into heterozygous zQ175 mouse primary neurons and humanized mutant (A) or endogenous mouse (B) HTT proteins were evaluated using the expanded polyglutamine human HTT <t>MSD</t> assay (antibody pair pAb146/MW1) or the mouse/rat HTT MSD assay (antibody pair pAb147/MAB2166), respectively. sh4, HTT targeting shRNA. scr6, scramble control shRNA. (C) Neuronal total tau protein levels measured using a commercially available MSD <t>ELISA-based</t> assay kit were monitored as loading control. Data are averages of n = 3 independent samples with correspondent standard deviations. ***, P
    Elisa Based Meso Scale Discovery Msd, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 86/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    GE Healthcare elisa based kit
    Polyol-responsiveness of anti-ERRα <t>mAbs.</t> <t>ELISA-elution</t> assays were performed to identify polyol-responsive mAbs (PR-mAbs). Antibodies were bound to ERRα-coated wells in 96-well plates, then incubated in TE control buffer or salt-polyol
    Elisa Based Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioTechniques eggers m high throughput microarray based enzyme linked immunosorbent assay elisa biotechniques
    Polyol-responsiveness of anti-ERRα <t>mAbs.</t> <t>ELISA-elution</t> assays were performed to identify polyol-responsive mAbs (PR-mAbs). Antibodies were bound to ERRα-coated wells in 96-well plates, then incubated in TE control buffer or salt-polyol
    Eggers M High Throughput Microarray Based Enzyme Linked Immunosorbent Assay Elisa Biotechniques, supplied by BioTechniques, used in various techniques. Bioz Stars score: 85/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    CR57 recognizes a linear epitope on rabies virus glycoprotein. (A) CR57 was tested using an 8-mer peptide PEPSCAN-ELISA in a region of the rabies virus glycoprotein ectodomain identified using overlapping 15-mer peptides. (B) Alanine replacement scan through the core 8-mer peptide containing the minimal binding region as identified in panel A. Relative ELISA signals are shown for each peptide.

    Journal: Journal of Virology

    Article Title: Novel Rabies Virus-Neutralizing Epitope Recognized by Human Monoclonal Antibody: Fine Mapping and Escape Mutant Analysis †

    doi: 10.1128/JVI.79.8.4672-4678.2005

    Figure Lengend Snippet: CR57 recognizes a linear epitope on rabies virus glycoprotein. (A) CR57 was tested using an 8-mer peptide PEPSCAN-ELISA in a region of the rabies virus glycoprotein ectodomain identified using overlapping 15-mer peptides. (B) Alanine replacement scan through the core 8-mer peptide containing the minimal binding region as identified in panel A. Relative ELISA signals are shown for each peptide.

    Article Snippet: Binding of the antibodies to each linear peptide was tested in a PEPSCAN-based ELISA.

    Techniques: Enzyme-linked Immunosorbent Assay, Binding Assay

    cAMP ELISA

    Journal: Journal of molecular and cellular cardiology

    Article Title: Comparative Effects of Urocortins and Stresscopin on Cardiac Myocyte Contractility

    doi: 10.1016/j.yjmcc.2015.07.023

    Figure Lengend Snippet: cAMP ELISA

    Article Snippet: cAMP ELISAs were performed using the Monoclonal Anti-cAMP Antibody Based Direct cAMP ELISA Kit (non-acetylated version) from NewEast Biosciences (#80204) as per the manufacturer’s protocol.

    Techniques: Enzyme-linked Immunosorbent Assay

    Signal transducer and activator of transcription-1 (STAT1) phosphorylation induced by IFN-γ in PrEC, PC-3, DU-145, and LNCaP cells. The STAT1 phosphorylation was determined by cell-based ELISA technique. Each point represents the mean of cellular

    Journal:

    Article Title: Differential Expression of Secretory Phospholipases A2 in Normal and Malignant Prostate Cell Lines: Regulation by Cytokines, Cell Signaling Pathways, and Epigenetic Mechanisms

    doi:

    Figure Lengend Snippet: Signal transducer and activator of transcription-1 (STAT1) phosphorylation induced by IFN-γ in PrEC, PC-3, DU-145, and LNCaP cells. The STAT1 phosphorylation was determined by cell-based ELISA technique. Each point represents the mean of cellular

    Article Snippet: The phosphorylation of STAT1 was measured using a cell-based ELISA kit (RayBiotech, Inc., Norcross, GA) according to the manufacturer's instructions.

    Techniques: In-Cell ELISA

    CD98hc mediates adhesion-induced RhoA activation and matrix contraction to enable Fn matrix assembly. (A) RhoA activity was measured in an ELISA-based Rho assay in WT and CD98hc-deficient cells after plating on a 3D Fn matrix. The error bars represent SEM. The assay was repeated three times with similar results. Samples were also resolved by SDS-PAGE and immunoblotted with anti-RhoA antibody (total RhoA) to confirm that both WT and CD98hc-null MEFs express similar amounts of total RhoA (not depicted). (B) Activation of RhoA by LPA in WT and CD98hc-null MEFs. Adherent serum-starved CD98hc-null cells were treated with 1 μg/ml LPA or buffer, and RhoA activity was measured after 5 min. Values represent the mean and range of duplicate determinations. The assay was repeated twice with similar results. (C) Activation of RhoA bypasses the defect in matrix contraction in CD98hc-null cells. Clot contraction was measured 1 h after WT, CD98hc-null, and CD98hc-null MEFs were stimulated with LPA (see Materials and methods for details). Values represent the mean ± SEM of triplicate determinations. Depicted is one of two such experiments with identical results. (D) Activation of RhoA bypasses the defect in Fn matrix assembly in CD98hc-null cells. DOC-insoluble Fn produced by WT, CD98hc-null, and CD98hc-null MEFs treated with LPA was evaluated biochemically as described in Fig. 3 . CD98hc-null MEFs stimulated with LPA were able to assemble Fn into fibrils as efficiently as WT cells. Depicted are the means of triplicate measurements. The assay was repeated two times with similar results.

    Journal: The Journal of Cell Biology

    Article Title: CD98hc (SLC3A2) participates in fibronectin matrix assembly by mediating integrin signaling

    doi: 10.1083/jcb.200705090

    Figure Lengend Snippet: CD98hc mediates adhesion-induced RhoA activation and matrix contraction to enable Fn matrix assembly. (A) RhoA activity was measured in an ELISA-based Rho assay in WT and CD98hc-deficient cells after plating on a 3D Fn matrix. The error bars represent SEM. The assay was repeated three times with similar results. Samples were also resolved by SDS-PAGE and immunoblotted with anti-RhoA antibody (total RhoA) to confirm that both WT and CD98hc-null MEFs express similar amounts of total RhoA (not depicted). (B) Activation of RhoA by LPA in WT and CD98hc-null MEFs. Adherent serum-starved CD98hc-null cells were treated with 1 μg/ml LPA or buffer, and RhoA activity was measured after 5 min. Values represent the mean and range of duplicate determinations. The assay was repeated twice with similar results. (C) Activation of RhoA bypasses the defect in matrix contraction in CD98hc-null cells. Clot contraction was measured 1 h after WT, CD98hc-null, and CD98hc-null MEFs were stimulated with LPA (see Materials and methods for details). Values represent the mean ± SEM of triplicate determinations. Depicted is one of two such experiments with identical results. (D) Activation of RhoA bypasses the defect in Fn matrix assembly in CD98hc-null cells. DOC-insoluble Fn produced by WT, CD98hc-null, and CD98hc-null MEFs treated with LPA was evaluated biochemically as described in Fig. 3 . CD98hc-null MEFs stimulated with LPA were able to assemble Fn into fibrils as efficiently as WT cells. Depicted are the means of triplicate measurements. The assay was repeated two times with similar results.

    Article Snippet: RhoA activity was measured using a commercially available ELISA-based assay (G-LISA; Cytoskeleton, Inc.) according to the manufacturer's protocol.

    Techniques: Activation Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, SDS Page, Produced

    Altered intestinal microbiota augments pulmonary cytokine response to Sendai virus (SeV), but does not impair viral clearance. Mice were placed on reverse osmosis (RO) or RO + streptomycin water, and 2 weeks later inoculated with SeV. (A) RT polymerase chain reaction (PCR) quantification of SeV copy number in whole lungs at the indicated days post inoculation (PI) SeV ( n = 3–6 mice/day and group). (B) Expression of cytokines in the bronchoalveolar lavage fluid of mice 8 days PI SeV. Data are array results from a commercially available ELISA protein array (data combined from five separate experiments with n ≥ 2/group/experiment, with samples run in duplicate); note increased IL-6, IFNγ, and CCL2 (MCP-1) protein. (C) Message for Il6 and Ifnγ , but not Ccl2 , was increased in whole lung of SeV-infected mice that had received streptomycin. Specific cytokine mRNA levels were assessed by RT PCR in whole of lung of mice at day 8 PI SeV or UV inactivated SeV ( n = 3/group and treatment) and normalized to Gapdh copy number.

    Journal: Frontiers in Immunology

    Article Title: Intestinal Microbiota Disruption Reduces Regulatory T Cells and Increases Respiratory Viral Infection Mortality Through Increased IFNγ Production

    doi: 10.3389/fimmu.2018.01587

    Figure Lengend Snippet: Altered intestinal microbiota augments pulmonary cytokine response to Sendai virus (SeV), but does not impair viral clearance. Mice were placed on reverse osmosis (RO) or RO + streptomycin water, and 2 weeks later inoculated with SeV. (A) RT polymerase chain reaction (PCR) quantification of SeV copy number in whole lungs at the indicated days post inoculation (PI) SeV ( n = 3–6 mice/day and group). (B) Expression of cytokines in the bronchoalveolar lavage fluid of mice 8 days PI SeV. Data are array results from a commercially available ELISA protein array (data combined from five separate experiments with n ≥ 2/group/experiment, with samples run in duplicate); note increased IL-6, IFNγ, and CCL2 (MCP-1) protein. (C) Message for Il6 and Ifnγ , but not Ccl2 , was increased in whole lung of SeV-infected mice that had received streptomycin. Specific cytokine mRNA levels were assessed by RT PCR in whole of lung of mice at day 8 PI SeV or UV inactivated SeV ( n = 3/group and treatment) and normalized to Gapdh copy number.

    Article Snippet: IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-10, IL-12p70, IL-17, TNF, IFNγ, GMCSF, CCL2, CCL3, CCL5, CCL11, CCL17, CCL22, and CXCL1 were measured using an ELISA based array (Quansys Biosciences, Logan, UT, USA).

    Techniques: Mouse Assay, Polymerase Chain Reaction, Expressing, Enzyme-linked Immunosorbent Assay, Protein Array, Infection, Reverse Transcription Polymerase Chain Reaction

    ATG7 knockdown prevents RelA/p65 nuclear translocation and DNA binding, but not IκBα degradation or RelA/p65 phosphorylation. ( A ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and then treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and processed according to an ELISA-based DNA binding assay kit as described in the “ Materials and Methods ” section. Error bars represent mean ± S.E. (n = 4 for each condition). ( B C ) HPAEC were transfected with si-Con or si-ATG7 for 48 h and treated with thrombin (5 U/ml) for 1 h. Total cell lysates were analyzed by Western blot for ( B ) IκBα levels and ( C ) RelA/p65 phosphorylation. RelA/p65 was used as a loading control. Error bars represent mean ± S.E. (n = 3–4 for each condition). ( D ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and analyzed by Western blot to measure the level of RelA/p65, and TATA-binding protein (TBP) was used as a loading control for nuclear protein. Error bars represent mean ± S.E. (n = 3–4 for each condition).

    Journal: Scientific Reports

    Article Title: Autophagy protein ATG7 is a critical regulator of endothelial cell inflammation and permeability

    doi: 10.1038/s41598-020-70126-7

    Figure Lengend Snippet: ATG7 knockdown prevents RelA/p65 nuclear translocation and DNA binding, but not IκBα degradation or RelA/p65 phosphorylation. ( A ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and then treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and processed according to an ELISA-based DNA binding assay kit as described in the “ Materials and Methods ” section. Error bars represent mean ± S.E. (n = 4 for each condition). ( B C ) HPAEC were transfected with si-Con or si-ATG7 for 48 h and treated with thrombin (5 U/ml) for 1 h. Total cell lysates were analyzed by Western blot for ( B ) IκBα levels and ( C ) RelA/p65 phosphorylation. RelA/p65 was used as a loading control. Error bars represent mean ± S.E. (n = 3–4 for each condition). ( D ) HPAEC were transfected for 48 h with si-Con or si-ATG7 and treated with thrombin (5 U/ml) for 1 h. Nuclear extracts were obtained and analyzed by Western blot to measure the level of RelA/p65, and TATA-binding protein (TBP) was used as a loading control for nuclear protein. Error bars represent mean ± S.E. (n = 3–4 for each condition).

    Article Snippet: The DNA binding activity of RelA/p65 was determined using an ELISA-based DNA binding assay kit (Cayman Chemical, Ann Arbor, MI) as described .

    Techniques: Translocation Assay, Binding Assay, Transfection, Enzyme-linked Immunosorbent Assay, DNA Binding Assay, Western Blot

    Dynamic changes in whole-lung cytokines and chemokines were apparent in TLR9 +/+ and TLR9 −/− mice during invasive aspergillosis induced by resting and swollen conidia. (A to C) ELISA or Bioplex analysis of TNF-α

    Journal:

    Article Title: Toll-Like Receptor 9 Modulates Immune Responses to Aspergillus fumigatus Conidia in Immunodeficient and Allergic Mice ▿

    doi: 10.1128/IAI.00998-08

    Figure Lengend Snippet: Dynamic changes in whole-lung cytokines and chemokines were apparent in TLR9 +/+ and TLR9 −/− mice during invasive aspergillosis induced by resting and swollen conidia. (A to C) ELISA or Bioplex analysis of TNF-α

    Article Snippet: Murine IL-4, IL-5, IL-10, IL-13, gamma interferon (IFN-γ), transforming growth factor β (TGF-β), TNF-α, IL-12, CXCL10, CCL11, CXCL9, CCL21, CCL6, CCL2, CCL3, CCL5, CCL22, and CCL17 were determined in 50-μl samples from whole-lung homogenates using a standardized sandwich ELISA technique previously described in detail or through a bead-based multiple target sandwich ELISA system (Bioplex; Bio-Rad Laboratories, Hercules, CA).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    Divergent effects on whole-lung cytokine and chemokine levels were observed in A. fumigatus -sensitized TLR9 −/− mice after challenge with resting or swollen conidia. (A, C, and E) ELISA or Bioplex analyses of CCL2 (A), CXCL10 (C), and IL-17

    Journal:

    Article Title: Toll-Like Receptor 9 Modulates Immune Responses to Aspergillus fumigatus Conidia in Immunodeficient and Allergic Mice ▿

    doi: 10.1128/IAI.00998-08

    Figure Lengend Snippet: Divergent effects on whole-lung cytokine and chemokine levels were observed in A. fumigatus -sensitized TLR9 −/− mice after challenge with resting or swollen conidia. (A, C, and E) ELISA or Bioplex analyses of CCL2 (A), CXCL10 (C), and IL-17

    Article Snippet: Murine IL-4, IL-5, IL-10, IL-13, gamma interferon (IFN-γ), transforming growth factor β (TGF-β), TNF-α, IL-12, CXCL10, CCL11, CXCL9, CCL21, CCL6, CCL2, CCL3, CCL5, CCL22, and CCL17 were determined in 50-μl samples from whole-lung homogenates using a standardized sandwich ELISA technique previously described in detail or through a bead-based multiple target sandwich ELISA system (Bioplex; Bio-Rad Laboratories, Hercules, CA).

    Techniques: Mouse Assay, Enzyme-linked Immunosorbent Assay

    (A) Release of cytokines and sTREM-1 from cultured monocytes. For cell activation, primary monocytes were cultured in 24-well flat-bottom tissue culture plates in the presence of LPS (1 μg/ml). In some experiments, this stimulus was provided in combination with LP17 (10–100 ng/ml), control peptide (10–100 ng/ml), or rIL-10 (500 U/ml). To activate monocytes through TREM-1, an agonist anti–TREM-1 mAb (10 μg/ml) was added as indicated. Cell-free supernatants were analyzed for production of TNF-α, IL-1β, and sTREM-1 by ELISA or immunodot. All experiments were performed in triplicate and data are expressed as means (SEM). (a) Media; (b) LP17 10 ng/ml; (c) anti–TREM-1; (d) LPS; (e) LPS + anti–TREM-1; (f) LPS + LP17 10 ng/ml; (g) LPS + LP17 50 ng/ml; (h) LPS + LP17 100 ng/ml; (i) LPS + IL10. (B) Effect of LP17 on NF-κB activation. Monocytes were cultured for 24 h in the presence of E. coli LPS (O111:B4; 1 μg/ml), anti–TREM-1 mAb (10 μg/ml), and/or LP17 (100 ng/ml) as indicated, and the levels of NF-κB p50 and p65 were determined using an ELISA-based assay. Experiments were performed in triplicate, and data are expressed as means of optical densities (SEM).

    Journal: The Journal of Experimental Medicine

    Article Title: A Soluble Form of the Triggering Receptor Expressed on Myeloid Cells-1 Modulates the Inflammatory Response in Murine Sepsis

    doi: 10.1084/jem.20040708

    Figure Lengend Snippet: (A) Release of cytokines and sTREM-1 from cultured monocytes. For cell activation, primary monocytes were cultured in 24-well flat-bottom tissue culture plates in the presence of LPS (1 μg/ml). In some experiments, this stimulus was provided in combination with LP17 (10–100 ng/ml), control peptide (10–100 ng/ml), or rIL-10 (500 U/ml). To activate monocytes through TREM-1, an agonist anti–TREM-1 mAb (10 μg/ml) was added as indicated. Cell-free supernatants were analyzed for production of TNF-α, IL-1β, and sTREM-1 by ELISA or immunodot. All experiments were performed in triplicate and data are expressed as means (SEM). (a) Media; (b) LP17 10 ng/ml; (c) anti–TREM-1; (d) LPS; (e) LPS + anti–TREM-1; (f) LPS + LP17 10 ng/ml; (g) LPS + LP17 50 ng/ml; (h) LPS + LP17 100 ng/ml; (i) LPS + IL10. (B) Effect of LP17 on NF-κB activation. Monocytes were cultured for 24 h in the presence of E. coli LPS (O111:B4; 1 μg/ml), anti–TREM-1 mAb (10 μg/ml), and/or LP17 (100 ng/ml) as indicated, and the levels of NF-κB p50 and p65 were determined using an ELISA-based assay. Experiments were performed in triplicate, and data are expressed as means of optical densities (SEM).

    Article Snippet: To address the effect of LP17 on NF-κB activity in monocytes, an ELISA-based assay was performed (BD Mercury Transfactor kit; BD Biosciences).

    Techniques: Cell Culture, Activation Assay, Enzyme-linked Immunosorbent Assay

    Specificity of human mutant and mouse HTT detection. The adeno-associated AAV-shRNA expression vector AAV-SEWB-sh4 was transduced into heterozygous zQ175 mouse primary neurons and humanized mutant (A) or endogenous mouse (B) HTT proteins were evaluated using the expanded polyglutamine human HTT MSD assay (antibody pair pAb146/MW1) or the mouse/rat HTT MSD assay (antibody pair pAb147/MAB2166), respectively. sh4, HTT targeting shRNA. scr6, scramble control shRNA. (C) Neuronal total tau protein levels measured using a commercially available MSD ELISA-based assay kit were monitored as loading control. Data are averages of n = 3 independent samples with correspondent standard deviations. ***, P

    Journal: PLoS ONE

    Article Title: Quantification Assays for Total and Polyglutamine-Expanded Huntingtin Proteins

    doi: 10.1371/journal.pone.0096854

    Figure Lengend Snippet: Specificity of human mutant and mouse HTT detection. The adeno-associated AAV-shRNA expression vector AAV-SEWB-sh4 was transduced into heterozygous zQ175 mouse primary neurons and humanized mutant (A) or endogenous mouse (B) HTT proteins were evaluated using the expanded polyglutamine human HTT MSD assay (antibody pair pAb146/MW1) or the mouse/rat HTT MSD assay (antibody pair pAb147/MAB2166), respectively. sh4, HTT targeting shRNA. scr6, scramble control shRNA. (C) Neuronal total tau protein levels measured using a commercially available MSD ELISA-based assay kit were monitored as loading control. Data are averages of n = 3 independent samples with correspondent standard deviations. ***, P

    Article Snippet: Here, we have developed a panel of detection assays for soluble polyglutamine-expanded (mutant) and total (polyglutamine-independent) human HTT protein as well as the rodent HTT protein ortholog using the sensitive ELISA-based Meso Scale Discovery (MSD) electrochemiluminescence assay platform .

    Techniques: Mutagenesis, shRNA, Expressing, Plasmid Preparation, Enzyme-linked Immunosorbent Assay

    Polyol-responsiveness of anti-ERRα mAbs. ELISA-elution assays were performed to identify polyol-responsive mAbs (PR-mAbs). Antibodies were bound to ERRα-coated wells in 96-well plates, then incubated in TE control buffer or salt-polyol

    Journal: Protein expression and purification

    Article Title: Production and characterization of monoclonal antibodies to estrogen-related receptor alpha (ERR?) and use in immunoaffinity chromatography

    doi: 10.1016/j.pep.2012.04.020

    Figure Lengend Snippet: Polyol-responsiveness of anti-ERRα mAbs. ELISA-elution assays were performed to identify polyol-responsive mAbs (PR-mAbs). Antibodies were bound to ERRα-coated wells in 96-well plates, then incubated in TE control buffer or salt-polyol

    Article Snippet: Antibody isotyping was performed using an ELISA-based kit (HyClone, Logan, UT), and all nine mAbs were determined to be IgG subtypes.

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation