elisa kit Search Results


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  • 99
    Thermo Fisher elisa kits
    Elisa Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 8254 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore insulin
    Insulin, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 27454 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher elisa kit
    Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 6578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam elisa kit
    Elisa Kit, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 1611 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher enzyme linked immunosorbent assay elisa kits
    Enzyme Linked Immunosorbent Assay Elisa Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1997 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Abcam elisa kits
    Koumine (KM) enhances LPS-induced astrocyte autophagy and attenuates astrocyte activation, as well as inflammation response in LPS-exposed primary astrocytes. Cultured astrocytes were exposed to koumine (0, 25, 50, or 100 μM) for 12 h prior to exposure to LPS for an additional 24 h before testing. Astrocytes in the control group were incubated with standard culture medium lacking koumine and LPS. Western blot (A) and densitometric quantification of GFAP (B) , Beclin 1 (C) , LC3-I/LC3-II (D) , P62 (E) , and GAPDH were performed in LPS-exposed astrocytes. <t>Elisa</t> were performed to explore the LPS-induced production of <t>TNF-α</t> (F) and IL-1β (G) in cultured astrocytes. Data are presented as the mean ± SEM. of three independent experiments. ### P
    Elisa Kits, supplied by Abcam, used in various techniques. Bioz Stars score: 97/100, based on 1066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Crystal Chem Inc ultra sensitive mouse insulin elisa kit
    Koumine (KM) enhances LPS-induced astrocyte autophagy and attenuates astrocyte activation, as well as inflammation response in LPS-exposed primary astrocytes. Cultured astrocytes were exposed to koumine (0, 25, 50, or 100 μM) for 12 h prior to exposure to LPS for an additional 24 h before testing. Astrocytes in the control group were incubated with standard culture medium lacking koumine and LPS. Western blot (A) and densitometric quantification of GFAP (B) , Beclin 1 (C) , LC3-I/LC3-II (D) , P62 (E) , and GAPDH were performed in LPS-exposed astrocytes. <t>Elisa</t> were performed to explore the LPS-induced production of <t>TNF-α</t> (F) and IL-1β (G) in cultured astrocytes. Data are presented as the mean ± SEM. of three independent experiments. ### P
    Ultra Sensitive Mouse Insulin Elisa Kit, supplied by Crystal Chem Inc, used in various techniques. Bioz Stars score: 99/100, based on 1190 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Cloud-Clone elisa kit
    <t>AAT</t> decreases the concentration of eNOS, MMP9, and s-Flt in blood from PE animals . <t>ELISA</t> was used to detect the concentration of AAT (A) , eNOS (B) , MMP9 (C) , and s-Flt (D) in serum from PE or normal animals after indicated treatment. N = 8. * p
    Elisa Kit, supplied by Cloud-Clone, used in various techniques. Bioz Stars score: 99/100, based on 860 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Enzo Biochem elisa kit
    Secretion of <t>HSP60</t> and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by <t>ELISA</t> using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p
    Elisa Kit, supplied by Enzo Biochem, used in various techniques. Bioz Stars score: 92/100, based on 841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    USCN Life elisa kit
    <t>CFI</t> bioactivity in a cohort of subjects with staged AMD and non-AMD normals. Subjects with AMD were staged according to the AREDS classification [ 24 ]. CFI bioactivity was determined by measuring the conversion of C3b to iC3b using <t>ELISA</t> for iC3b. Plasma dilutions (0.01–25 μl) were exposed to 80 μg/ml of each CFH and C3b and incubated at 37°C for 2 hours. Levels of iC3b were measured using ELISA. Samples from the same individual were run in the same assay as singlets. To control for the multiple assays conducted, each assay included a control sample. Data were fitted to a 4-parameter logistic equation and EC 50 s estimated. These values were converted to bioactivity in U/ μ g of CFI (1 mU = 1 / EC 50 in pg/ml). The logarithm of the bioactivity for the experimental samples and control plasma samples were analyzed by a mixed-model ANCOVA, using the control sample as a covariate to correct for inter-run variability. Data are shown as the fitted means in the original scale ± SE estimated from the fitted values using the Taylor series expansion. N = 4 to 47 patients per group. Legends: AREDS, Age-Related Eye Disease Study stages I through III, GA, geographic atrophy, Wet-Inactive, wet active AMD.
    Elisa Kit, supplied by USCN Life, used in various techniques. Bioz Stars score: 92/100, based on 1303 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MyBiosource elisa kit
    Effects of the <t>β2-GPI</t> DNA vaccine and FK506 treatment on β2-GPI-specific Treg cell response. Spleen cells were purified from the different mouse groups on day 56 and stimulated with recombinant β2-GPI protein (10 μg/mL). After 96 h, (A) the percentage of Foxp3-expressing CD4+ T cells was determined by flow cytometry. The dot plot shows data from one representative mouse from each group. The bar graph represents the mean ± standard deviation (SD) of six mice from three independent experiments. (B) The culture supernatants were collected, and IL-10 and TGF-β production was analyzed in triplicate by <t>ELISA.</t> The data are presented as the means ± SD of triplicate assays from six mice/group. ns p > 0.05, **p
    Elisa Kit, supplied by MyBiosource, used in various techniques. Bioz Stars score: 92/100, based on 932 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ALPCO elisa kit
    Age- and sex-related changes of serum TE profiles and biomarkers. Concentrations of Mn ( A ), I ( B ), Cu ( C ), Fe ( D ), Se ( G ), and Zn ( K ) were analyzed in the serum of adult (24 weeks) and old (109-114 weeks) male and female C57BL/6Jrj mice (n = 4-5) receiving chow diet. Serum concentrations were determined using ICP-MS/MS ( A - D , G , K ). Further biomarkers were detected by <t>ELISA</t> ( E , F ) and fluorescent probes ( L ) to assess the Fe marker ferritin ( E ) and <t>transferrin</t> ( F ) as well as free Zn ( L ), respectively. The Se status was further validated by GPX activity ( H ) and relative Selenop levels ( I ), based on NADPH-consuming glutathione reductase coupled assay and Dot blot analysis, respectively. Statistical testing based on Two-Way ANOVA and post hoc analysis using Bonferroni’s test with * p
    Elisa Kit, supplied by ALPCO, used in various techniques. Bioz Stars score: 92/100, based on 904 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems elisa kits
    DEX inhibits expression and release of <t>IL-1α</t> by HCAECs in response to inflammatory stimuli. HCAECs were stimulated with 100 ng/ml of TNF-α, or 10 ng/ml of IL-1α or IL-1β, for 48 h in the presence and absence of 10 mg/ml IgG and 1000 nM DEX, alone or in combination. Protein concentrations of IL-1α in HCAEC culture supernatants ( a ) and mRNA levels of IL-1α in HCAECs ( b ) were measured by <t>ELISA</t> and qPCR, respectively. Whole-cell lysates of HCAECs were subjected to WB analysis of the expression of IL-1α and heat shock protein 90 (HSP90; as a loading control) ( c ). Data shown in a and b are the mean ± SD of triplicate samples. All data are representative of two individual experiments using HCAEC lots from different donors. ** P
    Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 18588 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    IDEXX elisa kit
    Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The <t>sdAb</t> of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect <t>ELISA.</t> PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.
    Elisa Kit, supplied by IDEXX, used in various techniques. Bioz Stars score: 99/100, based on 814 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore human mmp 2 elisa kit
    Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The <t>sdAb</t> of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect <t>ELISA.</t> PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.
    Human Mmp 2 Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher elisa
    Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The <t>sdAb</t> of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect <t>ELISA.</t> PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.
    Elisa, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 13759 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher amyloid beta 42 human elisa kit
    Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The <t>sdAb</t> of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect <t>ELISA.</t> PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.
    Amyloid Beta 42 Human Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 793 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    R&D Systems quantikine elisa kit
    IL‐6 serum concentration in COVID‐19 patients before and 48 hours after treatment with itolizumab. (a) Median IL‐6 levels in the 3 groups of COVID‐19 patients. All experiments were performed in duplicate (Human IL‐6 <t>Quantikine</t> <t>ELISA</t> Kit). (b) ROC curves of IL‐6 predictive value for COVID‐19 severity. The asterisks indicate statistically significant differences among the groups (** P
    Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 2359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore elisa kit
    IL‐6 serum concentration in COVID‐19 patients before and 48 hours after treatment with itolizumab. (a) Median IL‐6 levels in the 3 groups of COVID‐19 patients. All experiments were performed in duplicate (Human IL‐6 <t>Quantikine</t> <t>ELISA</t> Kit). (b) ROC curves of IL‐6 predictive value for COVID‐19 severity. The asterisks indicate statistically significant differences among the groups (** P
    Elisa Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 2068 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    BioVendor Instruments elisa kit
    IL‐6 serum concentration in COVID‐19 patients before and 48 hours after treatment with itolizumab. (a) Median IL‐6 levels in the 3 groups of COVID‐19 patients. All experiments were performed in duplicate (Human IL‐6 <t>Quantikine</t> <t>ELISA</t> Kit). (b) ROC curves of IL‐6 predictive value for COVID‐19 severity. The asterisks indicate statistically significant differences among the groups (** P
    Elisa Kit, supplied by BioVendor Instruments, used in various techniques. Bioz Stars score: 92/100, based on 578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    N/A
    Enzyme Linked ImmunoSorbent Assay ELISA for the measurement of Human Fetuin A Levels in Serum
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    DNA Damage AccuSignal ELISA Kit KOA0887
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    Image Search Results


    Koumine (KM) enhances LPS-induced astrocyte autophagy and attenuates astrocyte activation, as well as inflammation response in LPS-exposed primary astrocytes. Cultured astrocytes were exposed to koumine (0, 25, 50, or 100 μM) for 12 h prior to exposure to LPS for an additional 24 h before testing. Astrocytes in the control group were incubated with standard culture medium lacking koumine and LPS. Western blot (A) and densitometric quantification of GFAP (B) , Beclin 1 (C) , LC3-I/LC3-II (D) , P62 (E) , and GAPDH were performed in LPS-exposed astrocytes. Elisa were performed to explore the LPS-induced production of TNF-α (F) and IL-1β (G) in cultured astrocytes. Data are presented as the mean ± SEM. of three independent experiments. ### P

    Journal: Frontiers in Pharmacology

    Article Title: Koumine Decreases Astrocyte-Mediated Neuroinflammation and Enhances Autophagy, Contributing to Neuropathic Pain From Chronic Constriction Injury in Rats

    doi: 10.3389/fphar.2018.00989

    Figure Lengend Snippet: Koumine (KM) enhances LPS-induced astrocyte autophagy and attenuates astrocyte activation, as well as inflammation response in LPS-exposed primary astrocytes. Cultured astrocytes were exposed to koumine (0, 25, 50, or 100 μM) for 12 h prior to exposure to LPS for an additional 24 h before testing. Astrocytes in the control group were incubated with standard culture medium lacking koumine and LPS. Western blot (A) and densitometric quantification of GFAP (B) , Beclin 1 (C) , LC3-I/LC3-II (D) , P62 (E) , and GAPDH were performed in LPS-exposed astrocytes. Elisa were performed to explore the LPS-induced production of TNF-α (F) and IL-1β (G) in cultured astrocytes. Data are presented as the mean ± SEM. of three independent experiments. ### P

    Article Snippet: Total TNF-α, IL-6, and IL-1β levels were measured using ELISA Kits (Abcam, ab46070 for TNF-α and ab100768 for IL-1β, Cambridge, MA, United States) according to the manufacturer’s instructions.

    Techniques: Activation Assay, Cell Culture, Incubation, Western Blot, Enzyme-linked Immunosorbent Assay

    AAT decreases the concentration of eNOS, MMP9, and s-Flt in blood from PE animals . ELISA was used to detect the concentration of AAT (A) , eNOS (B) , MMP9 (C) , and s-Flt (D) in serum from PE or normal animals after indicated treatment. N = 8. * p

    Journal: Frontiers in Physiology

    Article Title: Alpha-1 Antitrypsin Prevents the Development of Preeclampsia Through Suppression of Oxidative Stress

    doi: 10.3389/fphys.2016.00176

    Figure Lengend Snippet: AAT decreases the concentration of eNOS, MMP9, and s-Flt in blood from PE animals . ELISA was used to detect the concentration of AAT (A) , eNOS (B) , MMP9 (C) , and s-Flt (D) in serum from PE or normal animals after indicated treatment. N = 8. * p

    Article Snippet: ELISA Kit for Alpha-1-Antitrypsin (AAT), ELISA Kit for Nitric Oxide Synthase 3, Endothelial (eNOS), ELISA Kit for Matrix Metalloproteinase 9 (MMP9), and ELISA Kit for soluble fms-like tyrosine kinase-1 (sFlt-1) were from Cloud-Clone Corp. (Houston, TX, USA); superoxide dismutase (SOD) activity assay kit and glutathione peroxidase (GPx) activity assay kit were from Biovision (Milpitas, California, USA); lipid peroxidation malondialdehyde (MDA) assay kit and reactive oxygen species (ROS) assay kit were from Beyotime (Shanghai, China).

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay

    AAT decreases the activity of ROS and the concentration of MDA, whereas increases the activity of SOD and GPx . ELISA was used to detect the actvity of ROS (A) , the concentration of MDA (B) , and the activity of SOD (C) , and GPx (D) in serum from all groups. N = 8. * p

    Journal: Frontiers in Physiology

    Article Title: Alpha-1 Antitrypsin Prevents the Development of Preeclampsia Through Suppression of Oxidative Stress

    doi: 10.3389/fphys.2016.00176

    Figure Lengend Snippet: AAT decreases the activity of ROS and the concentration of MDA, whereas increases the activity of SOD and GPx . ELISA was used to detect the actvity of ROS (A) , the concentration of MDA (B) , and the activity of SOD (C) , and GPx (D) in serum from all groups. N = 8. * p

    Article Snippet: ELISA Kit for Alpha-1-Antitrypsin (AAT), ELISA Kit for Nitric Oxide Synthase 3, Endothelial (eNOS), ELISA Kit for Matrix Metalloproteinase 9 (MMP9), and ELISA Kit for soluble fms-like tyrosine kinase-1 (sFlt-1) were from Cloud-Clone Corp. (Houston, TX, USA); superoxide dismutase (SOD) activity assay kit and glutathione peroxidase (GPx) activity assay kit were from Biovision (Milpitas, California, USA); lipid peroxidation malondialdehyde (MDA) assay kit and reactive oxygen species (ROS) assay kit were from Beyotime (Shanghai, China).

    Techniques: Activity Assay, Concentration Assay, Multiple Displacement Amplification, Enzyme-linked Immunosorbent Assay

    The expression of AAT was dramatically decreased in blood and placenta tissues from PE patients . (A) Representative images of IHC staining for AAT. Bar = 50 μm. (B) AAT expression was decreased in placenta from PE patients. (C) ELISA was used to detect the concentration of AAT in blood from PE patients. (D) Western blot analysis for PAK, phosphorylated PAK, STAT1, phosphorylated STAT1, p38 and phosphorylated p38 in human placenta. (E) Quantification of the band in (C) . * p

    Journal: Frontiers in Physiology

    Article Title: Alpha-1 Antitrypsin Prevents the Development of Preeclampsia Through Suppression of Oxidative Stress

    doi: 10.3389/fphys.2016.00176

    Figure Lengend Snippet: The expression of AAT was dramatically decreased in blood and placenta tissues from PE patients . (A) Representative images of IHC staining for AAT. Bar = 50 μm. (B) AAT expression was decreased in placenta from PE patients. (C) ELISA was used to detect the concentration of AAT in blood from PE patients. (D) Western blot analysis for PAK, phosphorylated PAK, STAT1, phosphorylated STAT1, p38 and phosphorylated p38 in human placenta. (E) Quantification of the band in (C) . * p

    Article Snippet: ELISA Kit for Alpha-1-Antitrypsin (AAT), ELISA Kit for Nitric Oxide Synthase 3, Endothelial (eNOS), ELISA Kit for Matrix Metalloproteinase 9 (MMP9), and ELISA Kit for soluble fms-like tyrosine kinase-1 (sFlt-1) were from Cloud-Clone Corp. (Houston, TX, USA); superoxide dismutase (SOD) activity assay kit and glutathione peroxidase (GPx) activity assay kit were from Biovision (Milpitas, California, USA); lipid peroxidation malondialdehyde (MDA) assay kit and reactive oxygen species (ROS) assay kit were from Beyotime (Shanghai, China).

    Techniques: Expressing, Immunohistochemistry, Staining, Enzyme-linked Immunosorbent Assay, Concentration Assay, Western Blot

    AMPK is responsible for M1 protecting against GalN/LPS-induced fulminant hepatitis a Representative western blots of liver tissues of AMPKα1 lox/lox , AMPKα2 lox/lox , AMPKα1 LS −/− , and AMPKα2 LS −/− mice using antibody against AMPKα1, AMPKα2, and β-actin. AMPKα1 lox/lox /AMPKα2 lox/lox , AMPKα1 LS −/− , and AMPKα2 LS −/− mice were injected with 400 mg/kg GalN plus 1 μg/kg LPS. b The mortality rates were observed at various time points within 48 h ( n = 10). c Blood samples were harvested at 4 h after GalN/LPS administration. Serum ALT and AST levels were determined ( n = 5–6). d AMPKα1 LS −/− mice were intraperitoneal injected with either vehicle or 30 mg/kg M1 every 8 h one time for three times. The mortality rates were observed at various time points within 48 h after injected with a lethal dose of GalN (400 mg/kg)/LPS (1 μg/kg) ( n = 9–10). e Blood and liver samples were harvested at 4 h after GalN/LPS administration. Serum ALT and AST levels were determined ( n = 5). f H E staining analysis of liver sections are shown. Scale bars: 50 μm. g Serum TNFα and IL-1β levels were assayed using ELISA method ( n = 8). Each value is mean ± S.E.M. * P

    Journal: Cell Death & Disease

    Article Title: AMP-activated protein kinase agonist N6-(3-hydroxyphenyl)adenosine protects against fulminant hepatitis by suppressing inflammation and apoptosis

    doi: 10.1038/s41419-017-0118-0

    Figure Lengend Snippet: AMPK is responsible for M1 protecting against GalN/LPS-induced fulminant hepatitis a Representative western blots of liver tissues of AMPKα1 lox/lox , AMPKα2 lox/lox , AMPKα1 LS −/− , and AMPKα2 LS −/− mice using antibody against AMPKα1, AMPKα2, and β-actin. AMPKα1 lox/lox /AMPKα2 lox/lox , AMPKα1 LS −/− , and AMPKα2 LS −/− mice were injected with 400 mg/kg GalN plus 1 μg/kg LPS. b The mortality rates were observed at various time points within 48 h ( n = 10). c Blood samples were harvested at 4 h after GalN/LPS administration. Serum ALT and AST levels were determined ( n = 5–6). d AMPKα1 LS −/− mice were intraperitoneal injected with either vehicle or 30 mg/kg M1 every 8 h one time for three times. The mortality rates were observed at various time points within 48 h after injected with a lethal dose of GalN (400 mg/kg)/LPS (1 μg/kg) ( n = 9–10). e Blood and liver samples were harvested at 4 h after GalN/LPS administration. Serum ALT and AST levels were determined ( n = 5). f H E staining analysis of liver sections are shown. Scale bars: 50 μm. g Serum TNFα and IL-1β levels were assayed using ELISA method ( n = 8). Each value is mean ± S.E.M. * P

    Article Snippet: Circulating levels of cytokines TNF-a, IL-1β, and HMGB1 were quantified using ELISA kits according to the manufacturer’s instructions (Cloud-clone Corporation, Houston, USA).

    Techniques: Western Blot, Mouse Assay, Injection, AST Assay, Staining, Enzyme-linked Immunosorbent Assay

    M1 suppresses hepatocytes apoptosis and inflammatory cytokines production caused by GalN/LPS C57BL/6J mice were intraperitoneal injected either with vehicle or 30 mg/kg M1 every 8 h one time for three times. Blood and liver tissues were collected at 4 h after GalN (400 mg/kg)/LPS (10 μg/kg) treatment. a Hepatocytes apoptosis were measured by TUNEL staining and the number of apoptotic cells were counted ( n = 5). Scale bars: 50 μm. b The levels of cleaved caspase 3, caspase 8, caspase 9, and PARP were measured by western blot ( n = 5). c The expression of hepatic genes Gadd45α, Gadd45β, and A20 were determined by QPCR ( n = 3). d Serum TNFα and IL-1β levels were assayed using ELISA method ( n = 8). e Hepatic TNFα and IL-1β mRNA expression were determined by QPCR ( n = 4–6). f F4/80 staining of liver sections were conducted to indicate the filtration of macrophages. Scale bars: 50 μm. g Hepatic MPO activity was measured according to manufacturer’s instruction ( n = 5–6). Each value is mean ± S.E.M. * P

    Journal: Cell Death & Disease

    Article Title: AMP-activated protein kinase agonist N6-(3-hydroxyphenyl)adenosine protects against fulminant hepatitis by suppressing inflammation and apoptosis

    doi: 10.1038/s41419-017-0118-0

    Figure Lengend Snippet: M1 suppresses hepatocytes apoptosis and inflammatory cytokines production caused by GalN/LPS C57BL/6J mice were intraperitoneal injected either with vehicle or 30 mg/kg M1 every 8 h one time for three times. Blood and liver tissues were collected at 4 h after GalN (400 mg/kg)/LPS (10 μg/kg) treatment. a Hepatocytes apoptosis were measured by TUNEL staining and the number of apoptotic cells were counted ( n = 5). Scale bars: 50 μm. b The levels of cleaved caspase 3, caspase 8, caspase 9, and PARP were measured by western blot ( n = 5). c The expression of hepatic genes Gadd45α, Gadd45β, and A20 were determined by QPCR ( n = 3). d Serum TNFα and IL-1β levels were assayed using ELISA method ( n = 8). e Hepatic TNFα and IL-1β mRNA expression were determined by QPCR ( n = 4–6). f F4/80 staining of liver sections were conducted to indicate the filtration of macrophages. Scale bars: 50 μm. g Hepatic MPO activity was measured according to manufacturer’s instruction ( n = 5–6). Each value is mean ± S.E.M. * P

    Article Snippet: Circulating levels of cytokines TNF-a, IL-1β, and HMGB1 were quantified using ELISA kits according to the manufacturer’s instructions (Cloud-clone Corporation, Houston, USA).

    Techniques: Mouse Assay, Injection, TUNEL Assay, Staining, Western Blot, Expressing, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Filtration, Activity Assay

    Secretion of HSP60 and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by ELISA using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p

    Journal: Frontiers in Endocrinology

    Article Title: Physical Exercise Enhanced Heat Shock Protein 60 Expression and Attenuated Inflammation in the Adipose Tissue of Human Diabetic Obese

    doi: 10.3389/fendo.2018.00016

    Figure Lengend Snippet: Secretion of HSP60 and HSP60 autoantibodies into blood. Circulating levels of (A) HSP60 protein and (B) HSP60 auto-Abs were measured by ELISA using plasma samples from obese people without (ND) and with diabetes (D) before and after a 3-month physical exercise intervention ( n = 43 for each group). The p -value was determined using the Mann–Whitney test for comparisons between the diabetes and non-diabetes groups and using a paired t -test for intragroup comparisons before and after exercise. * denotes p

    Article Snippet: Plasma levels of anti-HSP60 IgG/A/M were measured using an ELISA kit (ADI-EKS-650, Enzo).

    Techniques: Enzyme-linked Immunosorbent Assay, MANN-WHITNEY

    CFI bioactivity in a cohort of subjects with staged AMD and non-AMD normals. Subjects with AMD were staged according to the AREDS classification [ 24 ]. CFI bioactivity was determined by measuring the conversion of C3b to iC3b using ELISA for iC3b. Plasma dilutions (0.01–25 μl) were exposed to 80 μg/ml of each CFH and C3b and incubated at 37°C for 2 hours. Levels of iC3b were measured using ELISA. Samples from the same individual were run in the same assay as singlets. To control for the multiple assays conducted, each assay included a control sample. Data were fitted to a 4-parameter logistic equation and EC 50 s estimated. These values were converted to bioactivity in U/ μ g of CFI (1 mU = 1 / EC 50 in pg/ml). The logarithm of the bioactivity for the experimental samples and control plasma samples were analyzed by a mixed-model ANCOVA, using the control sample as a covariate to correct for inter-run variability. Data are shown as the fitted means in the original scale ± SE estimated from the fitted values using the Taylor series expansion. N = 4 to 47 patients per group. Legends: AREDS, Age-Related Eye Disease Study stages I through III, GA, geographic atrophy, Wet-Inactive, wet active AMD.

    Journal: PLoS ONE

    Article Title: A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and Alzheimer’s disease

    doi: 10.1371/journal.pone.0195751

    Figure Lengend Snippet: CFI bioactivity in a cohort of subjects with staged AMD and non-AMD normals. Subjects with AMD were staged according to the AREDS classification [ 24 ]. CFI bioactivity was determined by measuring the conversion of C3b to iC3b using ELISA for iC3b. Plasma dilutions (0.01–25 μl) were exposed to 80 μg/ml of each CFH and C3b and incubated at 37°C for 2 hours. Levels of iC3b were measured using ELISA. Samples from the same individual were run in the same assay as singlets. To control for the multiple assays conducted, each assay included a control sample. Data were fitted to a 4-parameter logistic equation and EC 50 s estimated. These values were converted to bioactivity in U/ μ g of CFI (1 mU = 1 / EC 50 in pg/ml). The logarithm of the bioactivity for the experimental samples and control plasma samples were analyzed by a mixed-model ANCOVA, using the control sample as a covariate to correct for inter-run variability. Data are shown as the fitted means in the original scale ± SE estimated from the fitted values using the Taylor series expansion. N = 4 to 47 patients per group. Legends: AREDS, Age-Related Eye Disease Study stages I through III, GA, geographic atrophy, Wet-Inactive, wet active AMD.

    Article Snippet: ELISA detection of CFI, free Aβ and total bound Aβ CFI protein concentrations in human plasma were measured using a commercial ELISA kit from USCN Life Science (Wuhan, China), according to manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation

    CFI bioactivity in plasma samples of Alzheimer’s disease (AD). Plasma was collected from the phase I study of AD receiving anti-amyloid β antibody GSK933776 at 6 mg/Kg. CFI bioactivity was determined by measuring the conversion of C3b to iC3b using ELISA for iC3b. Plasma dilutions (0.01–25 nl) were exposed to CFI and CFH (80 μg/ml each) and incubated for 2 hours at 37°C. Samples from same individuals were run in singlets within the same assay. Each assay included a control sample. Data were fitted using a non-linear mixed model that used a reparametrized[ 25 ] 4-parameter logistic equation and EC 50 s were estimated. The values were converted to bioactivity of CFI-Units/μg of CFI (1mU = 1/EC 50 in pg). The logarithm of CFI bioactivity for study and control samples were analyzed by a non-linear mixed effects model using the control sample as a covariate to correct for inter-run variability. Data are presented as the geometric means ± SE estimated using the Taylor series expansion (n = 3–5 per group). Percentages shown in the graph were calculated f rom the integrated areas under the curve for each dosing period using the trapezoidal rule on the geometric means.

    Journal: PLoS ONE

    Article Title: A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and Alzheimer’s disease

    doi: 10.1371/journal.pone.0195751

    Figure Lengend Snippet: CFI bioactivity in plasma samples of Alzheimer’s disease (AD). Plasma was collected from the phase I study of AD receiving anti-amyloid β antibody GSK933776 at 6 mg/Kg. CFI bioactivity was determined by measuring the conversion of C3b to iC3b using ELISA for iC3b. Plasma dilutions (0.01–25 nl) were exposed to CFI and CFH (80 μg/ml each) and incubated for 2 hours at 37°C. Samples from same individuals were run in singlets within the same assay. Each assay included a control sample. Data were fitted using a non-linear mixed model that used a reparametrized[ 25 ] 4-parameter logistic equation and EC 50 s were estimated. The values were converted to bioactivity of CFI-Units/μg of CFI (1mU = 1/EC 50 in pg). The logarithm of CFI bioactivity for study and control samples were analyzed by a non-linear mixed effects model using the control sample as a covariate to correct for inter-run variability. Data are presented as the geometric means ± SE estimated using the Taylor series expansion (n = 3–5 per group). Percentages shown in the graph were calculated f rom the integrated areas under the curve for each dosing period using the trapezoidal rule on the geometric means.

    Article Snippet: ELISA detection of CFI, free Aβ and total bound Aβ CFI protein concentrations in human plasma were measured using a commercial ELISA kit from USCN Life Science (Wuhan, China), according to manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Incubation

    Anti-amyloid β (Aβ) antibody, GSK933776, effectively blocks Aβ’s ability to inhibit CFI bioactivity. Increasing doses of the antibody were preincubated with Aβ (30 μM) for 10 min at room temperature. CFI (1 μg/ml was added and incubated for 20 min at 37°C. CFH and C3b (80 μg/ml) were added and the mixture was incubated for an additional 30 min. Aliquots of the reaction mixture were analyzed for iC3b using ELISA. The curve in magenta shows the global fit of the two curves. The right dark red and dark blue bars show the effect of CFI + IgG1 (non-specific antibody control) on production of iC3b. The left 2 bars in lighter colors show the effect of CFI and IgG1 in presence of Aβ, i.e., reduction in CFI bioactivity. GSK933776 reduced Aβ’s ability to inhibit CFI in a dose-dependent manner (EC 50 ~ 20 μg/ml). Data are presented as means ± SEM of triplicate determinations for two experiments conducted over 2 separate weeks. A non-linear mixed effects model was used to estimate the EC 50 of the dose response curves using a reparametrized 4 parameter logistic equation [ 25 ]. Numbers in parentheses indicate 95% confidence intervals for the estimated EC 50 .

    Journal: PLoS ONE

    Article Title: A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and Alzheimer’s disease

    doi: 10.1371/journal.pone.0195751

    Figure Lengend Snippet: Anti-amyloid β (Aβ) antibody, GSK933776, effectively blocks Aβ’s ability to inhibit CFI bioactivity. Increasing doses of the antibody were preincubated with Aβ (30 μM) for 10 min at room temperature. CFI (1 μg/ml was added and incubated for 20 min at 37°C. CFH and C3b (80 μg/ml) were added and the mixture was incubated for an additional 30 min. Aliquots of the reaction mixture were analyzed for iC3b using ELISA. The curve in magenta shows the global fit of the two curves. The right dark red and dark blue bars show the effect of CFI + IgG1 (non-specific antibody control) on production of iC3b. The left 2 bars in lighter colors show the effect of CFI and IgG1 in presence of Aβ, i.e., reduction in CFI bioactivity. GSK933776 reduced Aβ’s ability to inhibit CFI in a dose-dependent manner (EC 50 ~ 20 μg/ml). Data are presented as means ± SEM of triplicate determinations for two experiments conducted over 2 separate weeks. A non-linear mixed effects model was used to estimate the EC 50 of the dose response curves using a reparametrized 4 parameter logistic equation [ 25 ]. Numbers in parentheses indicate 95% confidence intervals for the estimated EC 50 .

    Article Snippet: ELISA detection of CFI, free Aβ and total bound Aβ CFI protein concentrations in human plasma were measured using a commercial ELISA kit from USCN Life Science (Wuhan, China), according to manufacturer’s instructions.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay

    Preincubation of CFI with amyloid β (1–42) (Aβ) reduces CFI bioactivity. CFI (10–30 000 ng/ml) was incubated with Aβ (200 μM) for 1 hour at room temperature. CFH (10 μg/ml) and C3b (80 μg/ml) were added to the reaction and incubated at 37°C for 30 min. Aliquots of the reaction mixture were analyzed for iC3b by ELISA. Pre-incubation with Aβ causes a statistically significant shift of the curve to the right and approximately, a 5-fold decrease in CFI bioactivity. Data are presented as means ± SEM in triplicate wells. A non-linear mixed effects model was used to estimate the ratio of EC 50 for the dose response curves using a reparametrized 4 parameter logistic equation [ 25 ]. Number in parentheses indicate 95% confidence intervals for the estimated EC 50 ratio or EC 50 in ng/ml.

    Journal: PLoS ONE

    Article Title: A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and Alzheimer’s disease

    doi: 10.1371/journal.pone.0195751

    Figure Lengend Snippet: Preincubation of CFI with amyloid β (1–42) (Aβ) reduces CFI bioactivity. CFI (10–30 000 ng/ml) was incubated with Aβ (200 μM) for 1 hour at room temperature. CFH (10 μg/ml) and C3b (80 μg/ml) were added to the reaction and incubated at 37°C for 30 min. Aliquots of the reaction mixture were analyzed for iC3b by ELISA. Pre-incubation with Aβ causes a statistically significant shift of the curve to the right and approximately, a 5-fold decrease in CFI bioactivity. Data are presented as means ± SEM in triplicate wells. A non-linear mixed effects model was used to estimate the ratio of EC 50 for the dose response curves using a reparametrized 4 parameter logistic equation [ 25 ]. Number in parentheses indicate 95% confidence intervals for the estimated EC 50 ratio or EC 50 in ng/ml.

    Article Snippet: ELISA detection of CFI, free Aβ and total bound Aβ CFI protein concentrations in human plasma were measured using a commercial ELISA kit from USCN Life Science (Wuhan, China), according to manufacturer’s instructions.

    Techniques: Incubation, Enzyme-linked Immunosorbent Assay

    Measurement of CFI bioactivity. CFI bioactivity can be measured in plasma samples using an adapted assay that uses diluted plasma as the source of CFI. CFI bioactivity is temperature sensitive as heating the samples to 60° C abolishes the ability of the sample to generate iC3b (green symbol). Under the configuration of the assay, one can estimate the concentration of plasma that achieve half the amount of iC3b (i.e., 2.3 nl). We define this volume as 1 mU of CFI bioactivity. CFI bioactivity can be measured by determining the concentration of CFI protein in the same sample with an ELISA and correct the activity values by the amount of protein present in the sample, hence CFI bioactivity per unit of protein.

    Journal: PLoS ONE

    Article Title: A monoclonal antibody targeting amyloid β (Aβ) restores complement factor I bioactivity: Potential implications in age-related macular degeneration and Alzheimer’s disease

    doi: 10.1371/journal.pone.0195751

    Figure Lengend Snippet: Measurement of CFI bioactivity. CFI bioactivity can be measured in plasma samples using an adapted assay that uses diluted plasma as the source of CFI. CFI bioactivity is temperature sensitive as heating the samples to 60° C abolishes the ability of the sample to generate iC3b (green symbol). Under the configuration of the assay, one can estimate the concentration of plasma that achieve half the amount of iC3b (i.e., 2.3 nl). We define this volume as 1 mU of CFI bioactivity. CFI bioactivity can be measured by determining the concentration of CFI protein in the same sample with an ELISA and correct the activity values by the amount of protein present in the sample, hence CFI bioactivity per unit of protein.

    Article Snippet: ELISA detection of CFI, free Aβ and total bound Aβ CFI protein concentrations in human plasma were measured using a commercial ELISA kit from USCN Life Science (Wuhan, China), according to manufacturer’s instructions.

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay, Activity Assay

    Effects of the β2-GPI DNA vaccine and FK506 treatment on β2-GPI-specific Treg cell response. Spleen cells were purified from the different mouse groups on day 56 and stimulated with recombinant β2-GPI protein (10 μg/mL). After 96 h, (A) the percentage of Foxp3-expressing CD4+ T cells was determined by flow cytometry. The dot plot shows data from one representative mouse from each group. The bar graph represents the mean ± standard deviation (SD) of six mice from three independent experiments. (B) The culture supernatants were collected, and IL-10 and TGF-β production was analyzed in triplicate by ELISA. The data are presented as the means ± SD of triplicate assays from six mice/group. ns p > 0.05, **p

    Journal: PLoS ONE

    Article Title: Tolerogenic β2-glycoprotein I DNA vaccine and FK506 as an adjuvant attenuates experimental obstetric antiphospholipid syndrome

    doi: 10.1371/journal.pone.0198821

    Figure Lengend Snippet: Effects of the β2-GPI DNA vaccine and FK506 treatment on β2-GPI-specific Treg cell response. Spleen cells were purified from the different mouse groups on day 56 and stimulated with recombinant β2-GPI protein (10 μg/mL). After 96 h, (A) the percentage of Foxp3-expressing CD4+ T cells was determined by flow cytometry. The dot plot shows data from one representative mouse from each group. The bar graph represents the mean ± standard deviation (SD) of six mice from three independent experiments. (B) The culture supernatants were collected, and IL-10 and TGF-β production was analyzed in triplicate by ELISA. The data are presented as the means ± SD of triplicate assays from six mice/group. ns p > 0.05, **p

    Article Snippet: Anti-β2-GPI IgG concentrations were measured with an ELISA kit (MyBioSource, San Diego, CA) according to the manufacturer's protocol.

    Techniques: Purification, Recombinant, Expressing, Flow Cytometry, Cytometry, Standard Deviation, Mouse Assay, Enzyme-linked Immunosorbent Assay

    Effects of the β2-GPI DNA vaccine and FK506 treatment on cytokine production. Spleen cells were purified from the different mouse groups on day 56 and stimulated with recombinant β2-GPI protein (10 μg/mL). After 96 h, (A) the culture supernatants were collected, and interferon (IFN)- γ, interleukin (IL)-4 and IL-17A production was analyzed in triplicate by ELISA. The data are presented as the means ± standard deviation (SD) of triplicate assays from six mice/group. (B) The percentages of IFN-γ-expressing CD4+ T cells, IL-4-expressing CD4+ T cells and IL-17A-expressing CD4+ T cells were determined by flow cytometry. The dot plot shows data from one representative mouse from each group. The bar graph represents the mean ± SD of six mice from three independent experiments. ns p > 0.05, *p

    Journal: PLoS ONE

    Article Title: Tolerogenic β2-glycoprotein I DNA vaccine and FK506 as an adjuvant attenuates experimental obstetric antiphospholipid syndrome

    doi: 10.1371/journal.pone.0198821

    Figure Lengend Snippet: Effects of the β2-GPI DNA vaccine and FK506 treatment on cytokine production. Spleen cells were purified from the different mouse groups on day 56 and stimulated with recombinant β2-GPI protein (10 μg/mL). After 96 h, (A) the culture supernatants were collected, and interferon (IFN)- γ, interleukin (IL)-4 and IL-17A production was analyzed in triplicate by ELISA. The data are presented as the means ± standard deviation (SD) of triplicate assays from six mice/group. (B) The percentages of IFN-γ-expressing CD4+ T cells, IL-4-expressing CD4+ T cells and IL-17A-expressing CD4+ T cells were determined by flow cytometry. The dot plot shows data from one representative mouse from each group. The bar graph represents the mean ± SD of six mice from three independent experiments. ns p > 0.05, *p

    Article Snippet: Anti-β2-GPI IgG concentrations were measured with an ELISA kit (MyBioSource, San Diego, CA) according to the manufacturer's protocol.

    Techniques: Purification, Recombinant, Enzyme-linked Immunosorbent Assay, Standard Deviation, Mouse Assay, Expressing, Flow Cytometry, Cytometry

    Effects of the β2-GPI DNA vaccine and FK506 treatment on IgG anti-β2-GPI antibody levels. Serum samples were obtained from each mouse group on day 56, and IgG anti-β2-GPI antibody levels were analyzed by ELISA. The data are presented as the means ± standard deviation of triplicate assays from six mice/group. ns p > 0.05, *p

    Journal: PLoS ONE

    Article Title: Tolerogenic β2-glycoprotein I DNA vaccine and FK506 as an adjuvant attenuates experimental obstetric antiphospholipid syndrome

    doi: 10.1371/journal.pone.0198821

    Figure Lengend Snippet: Effects of the β2-GPI DNA vaccine and FK506 treatment on IgG anti-β2-GPI antibody levels. Serum samples were obtained from each mouse group on day 56, and IgG anti-β2-GPI antibody levels were analyzed by ELISA. The data are presented as the means ± standard deviation of triplicate assays from six mice/group. ns p > 0.05, *p

    Article Snippet: Anti-β2-GPI IgG concentrations were measured with an ELISA kit (MyBioSource, San Diego, CA) according to the manufacturer's protocol.

    Techniques: Enzyme-linked Immunosorbent Assay, Standard Deviation, Mouse Assay

    Age- and sex-related changes of serum TE profiles and biomarkers. Concentrations of Mn ( A ), I ( B ), Cu ( C ), Fe ( D ), Se ( G ), and Zn ( K ) were analyzed in the serum of adult (24 weeks) and old (109-114 weeks) male and female C57BL/6Jrj mice (n = 4-5) receiving chow diet. Serum concentrations were determined using ICP-MS/MS ( A - D , G , K ). Further biomarkers were detected by ELISA ( E , F ) and fluorescent probes ( L ) to assess the Fe marker ferritin ( E ) and transferrin ( F ) as well as free Zn ( L ), respectively. The Se status was further validated by GPX activity ( H ) and relative Selenop levels ( I ), based on NADPH-consuming glutathione reductase coupled assay and Dot blot analysis, respectively. Statistical testing based on Two-Way ANOVA and post hoc analysis using Bonferroni’s test with * p

    Journal: Aging (Albany NY)

    Article Title: Aging affects sex- and organ-specific trace element profiles in mice

    doi: 10.18632/aging.103572

    Figure Lengend Snippet: Age- and sex-related changes of serum TE profiles and biomarkers. Concentrations of Mn ( A ), I ( B ), Cu ( C ), Fe ( D ), Se ( G ), and Zn ( K ) were analyzed in the serum of adult (24 weeks) and old (109-114 weeks) male and female C57BL/6Jrj mice (n = 4-5) receiving chow diet. Serum concentrations were determined using ICP-MS/MS ( A - D , G , K ). Further biomarkers were detected by ELISA ( E , F ) and fluorescent probes ( L ) to assess the Fe marker ferritin ( E ) and transferrin ( F ) as well as free Zn ( L ), respectively. The Se status was further validated by GPX activity ( H ) and relative Selenop levels ( I ), based on NADPH-consuming glutathione reductase coupled assay and Dot blot analysis, respectively. Statistical testing based on Two-Way ANOVA and post hoc analysis using Bonferroni’s test with * p

    Article Snippet: ELISA for ferritin and transferrinThe concentrations of ferritin and transferrin were determined using an ELISA kit (ALPCO, Salem, USA) according to the manufacturer's instructions.

    Techniques: Mouse Assay, Tandem Mass Spectroscopy, Enzyme-linked Immunosorbent Assay, Marker, Activity Assay, Dot Blot

    Effect of increasing doses of TGFβ1 on 4-dione and T production in prostate stromal 6S cells. 6S cells were plated onto 60-mm plates at a density of 6.0 × 10 5 cells/plate with normal medium. After 24 h the medium was replaced with 2.5 mL new treatment medium containing 100 nM DHEA and 10–200 pM TGFβ1, and the cells were cultured for 48 h before the media was collected for ELISA of 4-dione (A) and T (B). To distinguish action of TGFβ1 on the conversion of 4-dione to T in 6S cells (3C), cells were treated as above then final treatment consisted of 10 nM 4-dione and 100 pM TGFβ1, for 48 h. Conditioned media were collected and T concentration was measured by ELISA. All results shown in A–C represent mean ± SD obtained from three independent experiments, with triplicate in each. * P

    Journal: The Journal of steroid biochemistry and molecular biology

    Article Title: TGFβ1 alters androgenic metabolites and hydroxysteroid dehydrogenase enzyme expression in human prostate reactive stromal primary cells: Is steroid metabolism altered by prostate reactive stromal microenvironment?

    doi: 10.1016/j.jsbmb.2013.05.016

    Figure Lengend Snippet: Effect of increasing doses of TGFβ1 on 4-dione and T production in prostate stromal 6S cells. 6S cells were plated onto 60-mm plates at a density of 6.0 × 10 5 cells/plate with normal medium. After 24 h the medium was replaced with 2.5 mL new treatment medium containing 100 nM DHEA and 10–200 pM TGFβ1, and the cells were cultured for 48 h before the media was collected for ELISA of 4-dione (A) and T (B). To distinguish action of TGFβ1 on the conversion of 4-dione to T in 6S cells (3C), cells were treated as above then final treatment consisted of 10 nM 4-dione and 100 pM TGFβ1, for 48 h. Conditioned media were collected and T concentration was measured by ELISA. All results shown in A–C represent mean ± SD obtained from three independent experiments, with triplicate in each. * P

    Article Snippet: DHEA metabolites were detected by using ELISA kits for free testosterone (cat # 11-FTSHU-E01-ALPCO, Salem, NH) and androstenedione (cat # DSL-10-3800-Beckman Coulter DSLabs, Webster, TX) according to the manufacturer’s instructions.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Concentration Assay

    DHEA metabolism in prostate stromal (6S) cells: TGFβ1 induced increase in 4-dione and T production in DHEA-treated prostate stromal 6S cells over time. Cells were plated onto 60-mm plates at a density of 6.0 × 10 5 cells/plate with normal medium. After 24 h, normal medium was replaced by 3 mL treatment medium (see Section 2) and the cells were cultured for further 24 h before stimulation. The medium was replaced with 2.5 mL of new treatment medium containing 100 nM DHEA plus or minus 100 pM TGFβ1, and the cells were cultured for 6, 12, 24, 48 and 72 h before the media were collected. The amount of 4-dione (A) and T (B) in the media was analyzed by ELISA. Control values of stromal metabolism are represented by hatched bars. Solid bars represent induction of 4-dione or T by TGFβ1. The results represent mean ± SD obtained from three independent experiments, with triplicate samples in each. * P

    Journal: The Journal of steroid biochemistry and molecular biology

    Article Title: TGFβ1 alters androgenic metabolites and hydroxysteroid dehydrogenase enzyme expression in human prostate reactive stromal primary cells: Is steroid metabolism altered by prostate reactive stromal microenvironment?

    doi: 10.1016/j.jsbmb.2013.05.016

    Figure Lengend Snippet: DHEA metabolism in prostate stromal (6S) cells: TGFβ1 induced increase in 4-dione and T production in DHEA-treated prostate stromal 6S cells over time. Cells were plated onto 60-mm plates at a density of 6.0 × 10 5 cells/plate with normal medium. After 24 h, normal medium was replaced by 3 mL treatment medium (see Section 2) and the cells were cultured for further 24 h before stimulation. The medium was replaced with 2.5 mL of new treatment medium containing 100 nM DHEA plus or minus 100 pM TGFβ1, and the cells were cultured for 6, 12, 24, 48 and 72 h before the media were collected. The amount of 4-dione (A) and T (B) in the media was analyzed by ELISA. Control values of stromal metabolism are represented by hatched bars. Solid bars represent induction of 4-dione or T by TGFβ1. The results represent mean ± SD obtained from three independent experiments, with triplicate samples in each. * P

    Article Snippet: DHEA metabolites were detected by using ELISA kits for free testosterone (cat # 11-FTSHU-E01-ALPCO, Salem, NH) and androstenedione (cat # DSL-10-3800-Beckman Coulter DSLabs, Webster, TX) according to the manufacturer’s instructions.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    Effects of TGFβ1 on DHEA metabolism in PrSC and LAPC4 cells. Normal primary stromal cells (PrSC) cells were plated onto 60-mm plates at a density of 6.0 × 10 5 cells/plate (or 2 × 10 6 for LAPC4 cells) with normal medium and allowed to attach for 24 h (PrSC; A and B) or 48 h (LAPC4 cells; C and D). Then, normal medium was replaced by 3 mL treatment medium, and the cells were cultured for further 24 h. Cells were then treated with 2.5 mL of new treatment medium consisting of 100 nM DHEA or DHEA plus 100 pM TGFβ1 for 48 h. The media were collected and the concentrations of 4-dione (A and C) and T (B and D) were accessed by ELISA. All results shown represent mean ± SD obtained from three independent experiments, with triplicate in each *** P

    Journal: The Journal of steroid biochemistry and molecular biology

    Article Title: TGFβ1 alters androgenic metabolites and hydroxysteroid dehydrogenase enzyme expression in human prostate reactive stromal primary cells: Is steroid metabolism altered by prostate reactive stromal microenvironment?

    doi: 10.1016/j.jsbmb.2013.05.016

    Figure Lengend Snippet: Effects of TGFβ1 on DHEA metabolism in PrSC and LAPC4 cells. Normal primary stromal cells (PrSC) cells were plated onto 60-mm plates at a density of 6.0 × 10 5 cells/plate (or 2 × 10 6 for LAPC4 cells) with normal medium and allowed to attach for 24 h (PrSC; A and B) or 48 h (LAPC4 cells; C and D). Then, normal medium was replaced by 3 mL treatment medium, and the cells were cultured for further 24 h. Cells were then treated with 2.5 mL of new treatment medium consisting of 100 nM DHEA or DHEA plus 100 pM TGFβ1 for 48 h. The media were collected and the concentrations of 4-dione (A and C) and T (B and D) were accessed by ELISA. All results shown represent mean ± SD obtained from three independent experiments, with triplicate in each *** P

    Article Snippet: DHEA metabolites were detected by using ELISA kits for free testosterone (cat # 11-FTSHU-E01-ALPCO, Salem, NH) and androstenedione (cat # DSL-10-3800-Beckman Coulter DSLabs, Webster, TX) according to the manufacturer’s instructions.

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay

    DEX inhibits expression and release of IL-1α by HCAECs in response to inflammatory stimuli. HCAECs were stimulated with 100 ng/ml of TNF-α, or 10 ng/ml of IL-1α or IL-1β, for 48 h in the presence and absence of 10 mg/ml IgG and 1000 nM DEX, alone or in combination. Protein concentrations of IL-1α in HCAEC culture supernatants ( a ) and mRNA levels of IL-1α in HCAECs ( b ) were measured by ELISA and qPCR, respectively. Whole-cell lysates of HCAECs were subjected to WB analysis of the expression of IL-1α and heat shock protein 90 (HSP90; as a loading control) ( c ). Data shown in a and b are the mean ± SD of triplicate samples. All data are representative of two individual experiments using HCAEC lots from different donors. ** P

    Journal: Pediatric Rheumatology Online Journal

    Article Title: Functional benefits of corticosteroid and IVIG combination therapy in a coronary artery endothelial cell model of Kawasaki disease

    doi: 10.1186/s12969-020-00461-6

    Figure Lengend Snippet: DEX inhibits expression and release of IL-1α by HCAECs in response to inflammatory stimuli. HCAECs were stimulated with 100 ng/ml of TNF-α, or 10 ng/ml of IL-1α or IL-1β, for 48 h in the presence and absence of 10 mg/ml IgG and 1000 nM DEX, alone or in combination. Protein concentrations of IL-1α in HCAEC culture supernatants ( a ) and mRNA levels of IL-1α in HCAECs ( b ) were measured by ELISA and qPCR, respectively. Whole-cell lysates of HCAECs were subjected to WB analysis of the expression of IL-1α and heat shock protein 90 (HSP90; as a loading control) ( c ). Data shown in a and b are the mean ± SD of triplicate samples. All data are representative of two individual experiments using HCAEC lots from different donors. ** P

    Article Snippet: ElisaThe concentrations of HMGB1, IL-1α, IL-6 and G-CSF proteins in cell-free supernatants were measured with specific ELISA kits (R & D Systems; Minneapolis, MN, USA) in accordance with the manufacturer’s instructions.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Western Blot

    Inhibitory kinetics of DEX and high-dose IgG on cytokine-induced production/release of IL-6, G-CSF and IL-1α by HCAECs. HCAECs were stimulated with 100 ng/ml of TNF-α or 10 ng/ml of IL-1β alone (without drugs) for 72 h and then treated with 1000 nM of DEX and/or 10 mg/ml of IgG at 0, 12 and 24 h after stimulation. The cell supernatants were collected at 72 h after cytokine stimulation. The protein concentrations of IL-6, G-CSF and IL-1α in the culture supernatants were measured by ELISA. Data are shown as the mean ± SD of triplicate samples and are representative of two individual experiments using HCAEC lots from two different donors. ** P

    Journal: Pediatric Rheumatology Online Journal

    Article Title: Functional benefits of corticosteroid and IVIG combination therapy in a coronary artery endothelial cell model of Kawasaki disease

    doi: 10.1186/s12969-020-00461-6

    Figure Lengend Snippet: Inhibitory kinetics of DEX and high-dose IgG on cytokine-induced production/release of IL-6, G-CSF and IL-1α by HCAECs. HCAECs were stimulated with 100 ng/ml of TNF-α or 10 ng/ml of IL-1β alone (without drugs) for 72 h and then treated with 1000 nM of DEX and/or 10 mg/ml of IgG at 0, 12 and 24 h after stimulation. The cell supernatants were collected at 72 h after cytokine stimulation. The protein concentrations of IL-6, G-CSF and IL-1α in the culture supernatants were measured by ELISA. Data are shown as the mean ± SD of triplicate samples and are representative of two individual experiments using HCAEC lots from two different donors. ** P

    Article Snippet: ElisaThe concentrations of HMGB1, IL-1α, IL-6 and G-CSF proteins in cell-free supernatants were measured with specific ELISA kits (R & D Systems; Minneapolis, MN, USA) in accordance with the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay

    Effects of DEX and high-dose IgG on inflammatory cytokine-induced expression of IL-6 and G-CSF in HCAECs. HCAECs were stimulated with 100 ng/ml of TNF-α, or 10 ng/ml of IL-1α or IL-1β, for 48 h in the presence and absence of 10 mg/ml IgG and 1000 nM DEX, alone or in combination. Protein concentrations of IL-6 and G-CSF in the culture supernatants ( a ) and mRNA levels of IL-6 and G-CSF ( b ) in HCAECs were measured by ELISA and qPCR, respectively. Data are shown as the mean ± SD of triplicate samples and are representative of two individual experiments using HCAEC lots from two different donors. ** P

    Journal: Pediatric Rheumatology Online Journal

    Article Title: Functional benefits of corticosteroid and IVIG combination therapy in a coronary artery endothelial cell model of Kawasaki disease

    doi: 10.1186/s12969-020-00461-6

    Figure Lengend Snippet: Effects of DEX and high-dose IgG on inflammatory cytokine-induced expression of IL-6 and G-CSF in HCAECs. HCAECs were stimulated with 100 ng/ml of TNF-α, or 10 ng/ml of IL-1α or IL-1β, for 48 h in the presence and absence of 10 mg/ml IgG and 1000 nM DEX, alone or in combination. Protein concentrations of IL-6 and G-CSF in the culture supernatants ( a ) and mRNA levels of IL-6 and G-CSF ( b ) in HCAECs were measured by ELISA and qPCR, respectively. Data are shown as the mean ± SD of triplicate samples and are representative of two individual experiments using HCAEC lots from two different donors. ** P

    Article Snippet: ElisaThe concentrations of HMGB1, IL-1α, IL-6 and G-CSF proteins in cell-free supernatants were measured with specific ELISA kits (R & D Systems; Minneapolis, MN, USA) in accordance with the manufacturer’s instructions.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction

    DEX, but not high-dose IgG, inhibits cellular damage to, and HMGB1 protein release by, HCAECs in response to inflammatory stimuli. HCAECs were stimulated with 100 ng/ml of TNF-α, or 10 ng/ml of IL-1α or IL-1β, for 24 h in the presence and absence of 10 mg/ml IgG and 1000 nM DEX, alone or in combination. Protein concentrations of HMGB1 in the culture supernatants ( a ), and HMGB1 mRNA levels ( b ) and caspase 3/7 activities in HCAECs ( c ) were measured by ELISA, qPCR and the Caspase-Glo 3/7 Assay System, respectively. Data are shown as the mean ± SD of triplicate samples and are representative of two individual experiments using HCAEC lots from different donors. ** P

    Journal: Pediatric Rheumatology Online Journal

    Article Title: Functional benefits of corticosteroid and IVIG combination therapy in a coronary artery endothelial cell model of Kawasaki disease

    doi: 10.1186/s12969-020-00461-6

    Figure Lengend Snippet: DEX, but not high-dose IgG, inhibits cellular damage to, and HMGB1 protein release by, HCAECs in response to inflammatory stimuli. HCAECs were stimulated with 100 ng/ml of TNF-α, or 10 ng/ml of IL-1α or IL-1β, for 24 h in the presence and absence of 10 mg/ml IgG and 1000 nM DEX, alone or in combination. Protein concentrations of HMGB1 in the culture supernatants ( a ), and HMGB1 mRNA levels ( b ) and caspase 3/7 activities in HCAECs ( c ) were measured by ELISA, qPCR and the Caspase-Glo 3/7 Assay System, respectively. Data are shown as the mean ± SD of triplicate samples and are representative of two individual experiments using HCAEC lots from different donors. ** P

    Article Snippet: ElisaThe concentrations of HMGB1, IL-1α, IL-6 and G-CSF proteins in cell-free supernatants were measured with specific ELISA kits (R & D Systems; Minneapolis, MN, USA) in accordance with the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Real-time Polymerase Chain Reaction, Caspase-Glo Assay

    The levels of MBD2, miR-301a-5p, CXCL12, and CXCR4 expression in blood samples from COPD patients. A qRT-PCR analysis was performed to measure the levels of ( A ) MBD2, ( B ) miR-301a-5p, ( C ) CXCL12, and ( D ) CXCR4 expression in PBMCs isolated from control (n = 20), R-COPD (n = 20), and AE-COPD (n = 20) subjects. ( E ) Serum miR-301a-5p expression levels in the above three groups were analyzed using qRT-PCR. ( F ) The relative levels of MBD2, CXCL12, and CXCR4 protein expression in serum samples from the above three groups were examined by Western blotting. ( G ) ELISA assays were conducted to analyze serum CXCL12 concentration levels. Data are expressed as the mean ± SD. *P

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: Mechanisms by Which the MBD2/miR-301a-5p/CXCL12/CXCR4 Pathway Regulates Acute Exacerbations of Chronic Obstructive Pulmonary Disease

    doi: 10.2147/COPD.S261522

    Figure Lengend Snippet: The levels of MBD2, miR-301a-5p, CXCL12, and CXCR4 expression in blood samples from COPD patients. A qRT-PCR analysis was performed to measure the levels of ( A ) MBD2, ( B ) miR-301a-5p, ( C ) CXCL12, and ( D ) CXCR4 expression in PBMCs isolated from control (n = 20), R-COPD (n = 20), and AE-COPD (n = 20) subjects. ( E ) Serum miR-301a-5p expression levels in the above three groups were analyzed using qRT-PCR. ( F ) The relative levels of MBD2, CXCL12, and CXCR4 protein expression in serum samples from the above three groups were examined by Western blotting. ( G ) ELISA assays were conducted to analyze serum CXCL12 concentration levels. Data are expressed as the mean ± SD. *P

    Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA)The concentrations of CXCL12 in serum and cell culture supernatant were determined using a commercially available ELISA kit (human CXCL12; R & D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, Quantitative RT-PCR, Isolation, Western Blot, Enzyme-linked Immunosorbent Assay, Concentration Assay

    MiR-301a-5p repressed CXCL12 expression by targeting the 3ʹUTR. ( A ) The predicted miR-301a-5p target sequence in the 3ʹUTR of CXCL12 mRNA. ( B ) Luciferase reporter assays were performed using HULEC-5a and HBE cells that had been co-transfected with miR-301a-5p or the NC together with WT or MUT CXCL12. Each treatment was performed in triplicate in three independent experiments. Results are expressed as relative luciferase activity (Firefly LUC/Renilla LUC) and were analyzed by Student’s t -test. HULEC-5a or HBE cells were transfected with miR-301a-5p mimics, the inhibitor or scramble, and then used for an analysis of miR-301a-5p and CXCL12 expression by qRT-PCR ( C-D ), as well as for an analysis of CXCL12 protein expression by Western blotting ( E ) and ELISA ( F ). Data are expressed as the mean ± SD. *** P

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: Mechanisms by Which the MBD2/miR-301a-5p/CXCL12/CXCR4 Pathway Regulates Acute Exacerbations of Chronic Obstructive Pulmonary Disease

    doi: 10.2147/COPD.S261522

    Figure Lengend Snippet: MiR-301a-5p repressed CXCL12 expression by targeting the 3ʹUTR. ( A ) The predicted miR-301a-5p target sequence in the 3ʹUTR of CXCL12 mRNA. ( B ) Luciferase reporter assays were performed using HULEC-5a and HBE cells that had been co-transfected with miR-301a-5p or the NC together with WT or MUT CXCL12. Each treatment was performed in triplicate in three independent experiments. Results are expressed as relative luciferase activity (Firefly LUC/Renilla LUC) and were analyzed by Student’s t -test. HULEC-5a or HBE cells were transfected with miR-301a-5p mimics, the inhibitor or scramble, and then used for an analysis of miR-301a-5p and CXCL12 expression by qRT-PCR ( C-D ), as well as for an analysis of CXCL12 protein expression by Western blotting ( E ) and ELISA ( F ). Data are expressed as the mean ± SD. *** P

    Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA)The concentrations of CXCL12 in serum and cell culture supernatant were determined using a commercially available ELISA kit (human CXCL12; R & D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, Sequencing, Luciferase, Transfection, Activity Assay, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    The regulatory effects of MBD2 on miR-301a-5p and CXCL12 expression in vitro. HULEC-5a or HBE cells were transfected with MBD2 or sh-MBD2, followed by CSE treatment. The levels of MBD2, miR-301a-5p, and CXCL12 expression in transfected ( A ) HULEC-5a and ( B ) HBE cells were measured by the qRT-PCR. ( C ) The relative levels of MBD2 and CXCL12 protein expression in transfected HULEC-5a and HBE cells were examined by Western blotting. ( D ) The extracellular CXCL12 concentrations in media from transfected HULEC-5a and HBE cells were determined by ELISA. Data are expressed as the mean ± SD. *** P

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: Mechanisms by Which the MBD2/miR-301a-5p/CXCL12/CXCR4 Pathway Regulates Acute Exacerbations of Chronic Obstructive Pulmonary Disease

    doi: 10.2147/COPD.S261522

    Figure Lengend Snippet: The regulatory effects of MBD2 on miR-301a-5p and CXCL12 expression in vitro. HULEC-5a or HBE cells were transfected with MBD2 or sh-MBD2, followed by CSE treatment. The levels of MBD2, miR-301a-5p, and CXCL12 expression in transfected ( A ) HULEC-5a and ( B ) HBE cells were measured by the qRT-PCR. ( C ) The relative levels of MBD2 and CXCL12 protein expression in transfected HULEC-5a and HBE cells were examined by Western blotting. ( D ) The extracellular CXCL12 concentrations in media from transfected HULEC-5a and HBE cells were determined by ELISA. Data are expressed as the mean ± SD. *** P

    Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA)The concentrations of CXCL12 in serum and cell culture supernatant were determined using a commercially available ELISA kit (human CXCL12; R & D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, In Vitro, Transfection, Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay

    The effects of CSE on MBD2, miR-301a-5p, CXCL12, and CXCR4 expression in vitro. HULEC-5a or HBE cells were treated with CSE. The levels of MBD2, miR-301a-5p, and CXCL12 expression in ( A ) HULEC-5a and ( B ) HBE cells were measured by the qRT-PCR. The extracellular CXCL12 concentration in medium was determined by ELISA Data are expressed as the mean ± SD. *** P

    Journal: International Journal of Chronic Obstructive Pulmonary Disease

    Article Title: Mechanisms by Which the MBD2/miR-301a-5p/CXCL12/CXCR4 Pathway Regulates Acute Exacerbations of Chronic Obstructive Pulmonary Disease

    doi: 10.2147/COPD.S261522

    Figure Lengend Snippet: The effects of CSE on MBD2, miR-301a-5p, CXCL12, and CXCR4 expression in vitro. HULEC-5a or HBE cells were treated with CSE. The levels of MBD2, miR-301a-5p, and CXCL12 expression in ( A ) HULEC-5a and ( B ) HBE cells were measured by the qRT-PCR. The extracellular CXCL12 concentration in medium was determined by ELISA Data are expressed as the mean ± SD. *** P

    Article Snippet: Enzyme-Linked Immunosorbent Assay (ELISA)The concentrations of CXCL12 in serum and cell culture supernatant were determined using a commercially available ELISA kit (human CXCL12; R & D Systems, Minneapolis, MN, USA) according to the manufacturer’s instructions.

    Techniques: Expressing, In Vitro, Quantitative RT-PCR, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The sdAb of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect ELISA. PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.

    Journal: International Journal of Nanomedicine

    Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

    doi: 10.2147/IJN.S218458

    Figure Lengend Snippet: Evaluation of specificity and reactivity of six representative sdAbs against SIV-NP. Notes: ( A ) The sdAb of one representative member of each family extracted from IPTG-induced E. coli TG1 colonies was checked for their specific binding to the immobilized SIV-NP using an indirect ELISA. PEDV-N was used as an irrelevant protein control. ( B ) The reactivity of six representative sdAbs against SIV-NP was detected by indirect ELISA. Abbreviations: sdAb, single-domain antibody; IPTG, isopropyl-β-D-thiogalactopyranoside; SIV, swine influenza virus; NP, nucleoprotein; PEDV, porcine epidemic diarrhea virus; N, nucleocapsid.

    Article Snippet: Evaluation of the Testing Consistency Among sdAb-ELISA, Commercial ELISA Kit, and Western Blot To compare consistency, sdAb-ELISA, the commercial ELISA kit (IDEXX Influenza A Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA) and Western blot were used to detect 120 clinical swine serum samples at dilution of 1:10.

    Techniques: Binding Assay, Indirect ELISA

    Schematic representation of isolating sdAbs from an immunized camel and development of sdAb-based blocking ELISA. Abbreviation: sdAb, single-domain antibody.

    Journal: International Journal of Nanomedicine

    Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

    doi: 10.2147/IJN.S218458

    Figure Lengend Snippet: Schematic representation of isolating sdAbs from an immunized camel and development of sdAb-based blocking ELISA. Abbreviation: sdAb, single-domain antibody.

    Article Snippet: Evaluation of the Testing Consistency Among sdAb-ELISA, Commercial ELISA Kit, and Western Blot To compare consistency, sdAb-ELISA, the commercial ELISA kit (IDEXX Influenza A Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA) and Western blot were used to detect 120 clinical swine serum samples at dilution of 1:10.

    Techniques: Blocking Assay, Enzyme-linked Immunosorbent Assay

    Consistency between sdAb-ELISA and commercial ELISA kit. Notes: The 120 swine serum samples were detected using (A) the commercial ELISA kit and (B) sdAb-ELISA, respectively. Abbreviation: sdAb, single-domain antibody.

    Journal: International Journal of Nanomedicine

    Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

    doi: 10.2147/IJN.S218458

    Figure Lengend Snippet: Consistency between sdAb-ELISA and commercial ELISA kit. Notes: The 120 swine serum samples were detected using (A) the commercial ELISA kit and (B) sdAb-ELISA, respectively. Abbreviation: sdAb, single-domain antibody.

    Article Snippet: Evaluation of the Testing Consistency Among sdAb-ELISA, Commercial ELISA Kit, and Western Blot To compare consistency, sdAb-ELISA, the commercial ELISA kit (IDEXX Influenza A Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA) and Western blot were used to detect 120 clinical swine serum samples at dilution of 1:10.

    Techniques: Enzyme-linked Immunosorbent Assay

    Evaluation of the discrepancy of testing results between sdAb-ELISA and the commercial ELISA kit by Western blot. Notes: ( A ) Western blot was performed to detect swine serum samples with discrepancy between sdAb-ELISA and the commercial ELISA kit. ( B ) The detection results of three indicated methods. Abbreviation: sdAb, single-domain antibody.

    Journal: International Journal of Nanomedicine

    Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

    doi: 10.2147/IJN.S218458

    Figure Lengend Snippet: Evaluation of the discrepancy of testing results between sdAb-ELISA and the commercial ELISA kit by Western blot. Notes: ( A ) Western blot was performed to detect swine serum samples with discrepancy between sdAb-ELISA and the commercial ELISA kit. ( B ) The detection results of three indicated methods. Abbreviation: sdAb, single-domain antibody.

    Article Snippet: Evaluation of the Testing Consistency Among sdAb-ELISA, Commercial ELISA Kit, and Western Blot To compare consistency, sdAb-ELISA, the commercial ELISA kit (IDEXX Influenza A Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA) and Western blot were used to detect 120 clinical swine serum samples at dilution of 1:10.

    Techniques: Enzyme-linked Immunosorbent Assay, Western Blot

    Determination of the optimal conditions of sdAb-ELISA. Notes: ( A ) A checkerboard titration was used to test the optimal concentration range of biotinylated sdAb5 for each dilution of SIV-NP. ( B ) After the optimal concentration of biotinylated sdAb5 for each concentration SIV-NP was determined using another checkboard titration, the optimal dilution of serum was determined using sdAb-ELISA. Abbreviations: sdAb-ELISA, single-domain antibody-based blocking ELISA; SIV, swine influenza virus; NP, nucleoprotein.

    Journal: International Journal of Nanomedicine

    Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

    doi: 10.2147/IJN.S218458

    Figure Lengend Snippet: Determination of the optimal conditions of sdAb-ELISA. Notes: ( A ) A checkerboard titration was used to test the optimal concentration range of biotinylated sdAb5 for each dilution of SIV-NP. ( B ) After the optimal concentration of biotinylated sdAb5 for each concentration SIV-NP was determined using another checkboard titration, the optimal dilution of serum was determined using sdAb-ELISA. Abbreviations: sdAb-ELISA, single-domain antibody-based blocking ELISA; SIV, swine influenza virus; NP, nucleoprotein.

    Article Snippet: Evaluation of the Testing Consistency Among sdAb-ELISA, Commercial ELISA Kit, and Western Blot To compare consistency, sdAb-ELISA, the commercial ELISA kit (IDEXX Influenza A Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA) and Western blot were used to detect 120 clinical swine serum samples at dilution of 1:10.

    Techniques: Enzyme-linked Immunosorbent Assay, Titration, Concentration Assay, Blocking Assay

    Specificity and sensitivity of sdAb-ELISA. Notes: ( A ) The sera against SIV, PCV2, PEDV, PRRSV, PRV, and CSFV were used to evaluate specificity of the sdAb-ELISA. ( B ) The detection limit of the commercial ELISA kit (left) and sdAb-ELISA (right) were determined for an anti-SIV reference swine positive serum. ( C ) Serum samples were diluted 1/80 and used to evaluate and compare the sensitivity of the commercial ELISA kit (top) and sdAb-ELISA (bottom). Abbreviations: SIV, swine influenza virus; PCV2, porcine circovirus type 2; PEDV, porcine epidemic diarrhea virus; PRRSV, porcine reproductive and respiratory syndrome virus; PRV, pseudorabies virus; CSFV, classical swine fever virus.

    Journal: International Journal of Nanomedicine

    Article Title: Biotinylated Single-Domain Antibody-Based Blocking ELISA for Detection of Antibodies Against Swine Influenza Virus

    doi: 10.2147/IJN.S218458

    Figure Lengend Snippet: Specificity and sensitivity of sdAb-ELISA. Notes: ( A ) The sera against SIV, PCV2, PEDV, PRRSV, PRV, and CSFV were used to evaluate specificity of the sdAb-ELISA. ( B ) The detection limit of the commercial ELISA kit (left) and sdAb-ELISA (right) were determined for an anti-SIV reference swine positive serum. ( C ) Serum samples were diluted 1/80 and used to evaluate and compare the sensitivity of the commercial ELISA kit (top) and sdAb-ELISA (bottom). Abbreviations: SIV, swine influenza virus; PCV2, porcine circovirus type 2; PEDV, porcine epidemic diarrhea virus; PRRSV, porcine reproductive and respiratory syndrome virus; PRV, pseudorabies virus; CSFV, classical swine fever virus.

    Article Snippet: Evaluation of the Testing Consistency Among sdAb-ELISA, Commercial ELISA Kit, and Western Blot To compare consistency, sdAb-ELISA, the commercial ELISA kit (IDEXX Influenza A Ab Test, IDEXX Laboratories, Inc., Westbrook, ME, USA) and Western blot were used to detect 120 clinical swine serum samples at dilution of 1:10.

    Techniques: Enzyme-linked Immunosorbent Assay

    IL‐6 serum concentration in COVID‐19 patients before and 48 hours after treatment with itolizumab. (a) Median IL‐6 levels in the 3 groups of COVID‐19 patients. All experiments were performed in duplicate (Human IL‐6 Quantikine ELISA Kit). (b) ROC curves of IL‐6 predictive value for COVID‐19 severity. The asterisks indicate statistically significant differences among the groups (** P

    Journal: Clinical & Translational Immunology

    Article Title: Treatment of COVID‐19 patients with the anti‐CD6 antibody itolizumab

    doi: 10.1002/cti2.1218

    Figure Lengend Snippet: IL‐6 serum concentration in COVID‐19 patients before and 48 hours after treatment with itolizumab. (a) Median IL‐6 levels in the 3 groups of COVID‐19 patients. All experiments were performed in duplicate (Human IL‐6 Quantikine ELISA Kit). (b) ROC curves of IL‐6 predictive value for COVID‐19 severity. The asterisks indicate statistically significant differences among the groups (** P

    Article Snippet: IL‐6 concentration was measured using a Quantikine ELISA Kit (S6050) from R & D Systems (Minneapolis, USA).

    Techniques: Concentration Assay, Enzyme-linked Immunosorbent Assay