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  • 99
    Thermo Fisher elisa kits
    Elisa Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/Thermo Fisher
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2021-04
    99/100 stars
      Buy from Supplier

    86
    R&D Systems elisa kits
    Effects of the LOX-1, CD36 and SR-A1 siRNAs on oxLDL-induced <t>IL-1β</t> productions and effects of oxLDL and Tan IIA on LOX-1 expression and LOX-1-mediated caspase-1activation/IL-1β release. ( A – C ) Effects of lentivirus-mediated LOX-1, CD36 and SR-A1 siRNAs (MOI = 30) on oxLDL-induced IL-1β productions in mouse macrophages measured by <t>ELISA.</t> ( D , E ) Effects of oxLDL on LOX-1 expression in macrophages measured by qRT-PCR and Western blot, respectively. ( F , G ) Effects of Tan IIA on oxLDL-induced LOX-1 expression determined by qRT-PCR and Western blot, respectively. ( H ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated IL-1β expression in macrophages. ( I ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated caspase-1 activation in macrophages.
    Elisa Kits, supplied by R&D Systems, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kits/product/R&D Systems
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kits - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

    94
    R&D Systems elisa kit
    Effects of the LOX-1, CD36 and SR-A1 siRNAs on oxLDL-induced <t>IL-1β</t> productions and effects of oxLDL and Tan IIA on LOX-1 expression and LOX-1-mediated caspase-1activation/IL-1β release. ( A – C ) Effects of lentivirus-mediated LOX-1, CD36 and SR-A1 siRNAs (MOI = 30) on oxLDL-induced IL-1β productions in mouse macrophages measured by <t>ELISA.</t> ( D , E ) Effects of oxLDL on LOX-1 expression in macrophages measured by qRT-PCR and Western blot, respectively. ( F , G ) Effects of Tan IIA on oxLDL-induced LOX-1 expression determined by qRT-PCR and Western blot, respectively. ( H ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated IL-1β expression in macrophages. ( I ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated caspase-1 activation in macrophages.
    Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/R&D Systems
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kit - by Bioz Stars, 2021-04
    94/100 stars
      Buy from Supplier

    92
    Thermo Fisher elisa kit
    Effects of the LOX-1, CD36 and SR-A1 siRNAs on oxLDL-induced <t>IL-1β</t> productions and effects of oxLDL and Tan IIA on LOX-1 expression and LOX-1-mediated caspase-1activation/IL-1β release. ( A – C ) Effects of lentivirus-mediated LOX-1, CD36 and SR-A1 siRNAs (MOI = 30) on oxLDL-induced IL-1β productions in mouse macrophages measured by <t>ELISA.</t> ( D , E ) Effects of oxLDL on LOX-1 expression in macrophages measured by qRT-PCR and Western blot, respectively. ( F , G ) Effects of Tan IIA on oxLDL-induced LOX-1 expression determined by qRT-PCR and Western blot, respectively. ( H ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated IL-1β expression in macrophages. ( I ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated caspase-1 activation in macrophages.
    Elisa Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/elisa kit/product/Thermo Fisher
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    elisa kit - by Bioz Stars, 2021-04
    92/100 stars
      Buy from Supplier

    N/A
    ELISA kit for detection of Human VEGFR1 in the research laboratory
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    ELISA kit for the detection of ssDNA in the research laboratory
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    ELISA kit for detection of Vaspin in the research laboratory
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    N/A
    A Protein Conformational Array PCA in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence
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    Effects of the LOX-1, CD36 and SR-A1 siRNAs on oxLDL-induced IL-1β productions and effects of oxLDL and Tan IIA on LOX-1 expression and LOX-1-mediated caspase-1activation/IL-1β release. ( A – C ) Effects of lentivirus-mediated LOX-1, CD36 and SR-A1 siRNAs (MOI = 30) on oxLDL-induced IL-1β productions in mouse macrophages measured by ELISA. ( D , E ) Effects of oxLDL on LOX-1 expression in macrophages measured by qRT-PCR and Western blot, respectively. ( F , G ) Effects of Tan IIA on oxLDL-induced LOX-1 expression determined by qRT-PCR and Western blot, respectively. ( H ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated IL-1β expression in macrophages. ( I ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated caspase-1 activation in macrophages.

    Journal: Aging (Albany NY)

    Article Title: Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

    doi: 10.18632/aging.202202

    Figure Lengend Snippet: Effects of the LOX-1, CD36 and SR-A1 siRNAs on oxLDL-induced IL-1β productions and effects of oxLDL and Tan IIA on LOX-1 expression and LOX-1-mediated caspase-1activation/IL-1β release. ( A – C ) Effects of lentivirus-mediated LOX-1, CD36 and SR-A1 siRNAs (MOI = 30) on oxLDL-induced IL-1β productions in mouse macrophages measured by ELISA. ( D , E ) Effects of oxLDL on LOX-1 expression in macrophages measured by qRT-PCR and Western blot, respectively. ( F , G ) Effects of Tan IIA on oxLDL-induced LOX-1 expression determined by qRT-PCR and Western blot, respectively. ( H ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated IL-1β expression in macrophages. ( I ) Effects of Tan IIA on oxLDL-induced LOX-1-mediated caspase-1 activation in macrophages.

    Article Snippet: The measurements of all the above cytokines, except for IL-1β, were performed using commercially available ELISA kits (R & D Systems) according to the manufactures’ instructions.

    Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Western Blot, Activation Assay

    Tan IIA inhibits oxLDL-induced CD36 expression by inhibiting Nrf2 expression and PPARγ activity. ( A , B ) Effects of oxLDL on CD36 expressions at mRNA levels measured by qRT-PCR and protein levels detected by Western blot in macrophage, respectively; GAPDH, glyceraldehyde-3-phosphate dehydrogenase as an internal control. ( C , D ) Effects of Tan IIA on oxLDL-induced CD36 expressions at mRNA levels and protein levels in macrophages, respectively. ( E ) Effects of Tan IIA on oxLDL-induced caspase-1 activation in the cells detected by Western blot. ( F ) Effects of Tan IIA on oxLDL-induced IL-1β productions measured by ELISA in macrophages. ( G , H ) Effects of Nrf2 and PPARγ gene knockdown by siRNA on CD36 expression on the cell membrane, respectively, detected by flow cytometry, presented by mean fluorescence intensity (MFI). ( I , J ) Effects of Tan IIA on oxLDL-mediated Nrf2 expression measured by RT-PCR and oxLDL-mediated PPARγ activity measured by a commercial kit in the cells.

    Journal: Aging (Albany NY)

    Article Title: Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

    doi: 10.18632/aging.202202

    Figure Lengend Snippet: Tan IIA inhibits oxLDL-induced CD36 expression by inhibiting Nrf2 expression and PPARγ activity. ( A , B ) Effects of oxLDL on CD36 expressions at mRNA levels measured by qRT-PCR and protein levels detected by Western blot in macrophage, respectively; GAPDH, glyceraldehyde-3-phosphate dehydrogenase as an internal control. ( C , D ) Effects of Tan IIA on oxLDL-induced CD36 expressions at mRNA levels and protein levels in macrophages, respectively. ( E ) Effects of Tan IIA on oxLDL-induced caspase-1 activation in the cells detected by Western blot. ( F ) Effects of Tan IIA on oxLDL-induced IL-1β productions measured by ELISA in macrophages. ( G , H ) Effects of Nrf2 and PPARγ gene knockdown by siRNA on CD36 expression on the cell membrane, respectively, detected by flow cytometry, presented by mean fluorescence intensity (MFI). ( I , J ) Effects of Tan IIA on oxLDL-mediated Nrf2 expression measured by RT-PCR and oxLDL-mediated PPARγ activity measured by a commercial kit in the cells.

    Article Snippet: The measurements of all the above cytokines, except for IL-1β, were performed using commercially available ELISA kits (R & D Systems) according to the manufactures’ instructions.

    Techniques: Expressing, Activity Assay, Quantitative RT-PCR, Western Blot, Activation Assay, Enzyme-linked Immunosorbent Assay, Flow Cytometry, Fluorescence, Reverse Transcription Polymerase Chain Reaction

    Tan IIA inhibits LPS- and oxLDL-induced pro-inflammatory cytokine and NLRP3 production in macrophages. ( A, B ) Effects of Tan IIA on LPS- or oxLDL-induced NF-κB P56 activity in the cytoplasm and nucleus of mouse B6 macrophages. ( C ) Effects of Tan IIA at the different doses (2.5, 5 and 10 μg/mL) on pro-IL-1β expressions by qRT-PCR detection in LPS- and oxLDL-induced mouse B6 macrophages. ( D , E ) Effects of Tan IIA on pro-IL-1β expressions detected by Western blot in LPS- and oxLDL-induced B6 macrophages, respectively. ( F , G ) Effects of Tan IIA on IL-6 expression in the cells by RT-PCR and in the culture medium by ELISA, respectively, in LPS- and oxLDL-induced B6 macrophages. ( H , I ) Effects of Tan IIA on TNFα expression in the cells by qRT-PCR and in the culture medium by ELISA, respectively, in LPS- and oxLDL-induced B6 macrophages. ( J ) Effects of Tan IIA on NLRP3 expressions by qRT-PCR detection in LPS- and oxLDL-induced mouse B6 macrophages. ( K , L ) Effects of Tan IIA on NLRP3 expressions in the culture medium by Western blot in LPS- and oxLDL-induced B6 macrophages, respectively.

    Journal: Aging (Albany NY)

    Article Title: Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

    doi: 10.18632/aging.202202

    Figure Lengend Snippet: Tan IIA inhibits LPS- and oxLDL-induced pro-inflammatory cytokine and NLRP3 production in macrophages. ( A, B ) Effects of Tan IIA on LPS- or oxLDL-induced NF-κB P56 activity in the cytoplasm and nucleus of mouse B6 macrophages. ( C ) Effects of Tan IIA at the different doses (2.5, 5 and 10 μg/mL) on pro-IL-1β expressions by qRT-PCR detection in LPS- and oxLDL-induced mouse B6 macrophages. ( D , E ) Effects of Tan IIA on pro-IL-1β expressions detected by Western blot in LPS- and oxLDL-induced B6 macrophages, respectively. ( F , G ) Effects of Tan IIA on IL-6 expression in the cells by RT-PCR and in the culture medium by ELISA, respectively, in LPS- and oxLDL-induced B6 macrophages. ( H , I ) Effects of Tan IIA on TNFα expression in the cells by qRT-PCR and in the culture medium by ELISA, respectively, in LPS- and oxLDL-induced B6 macrophages. ( J ) Effects of Tan IIA on NLRP3 expressions by qRT-PCR detection in LPS- and oxLDL-induced mouse B6 macrophages. ( K , L ) Effects of Tan IIA on NLRP3 expressions in the culture medium by Western blot in LPS- and oxLDL-induced B6 macrophages, respectively.

    Article Snippet: The measurements of all the above cytokines, except for IL-1β, were performed using commercially available ELISA kits (R & D Systems) according to the manufactures’ instructions.

    Techniques: Activity Assay, Quantitative RT-PCR, Western Blot, Expressing, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay

    Tan IIA inhibits oxLDL-induced macrophage lysosomal damage and cathepsin B release. ( A ) Fluorescence confocal assay for oxLDL-induced cathepsin B release from lysosome of mouse B6 macrophages or cathepsin B -/- B6 macrophages under the different treatments, respectively. The cells were treated with oxLDL (50 μg/mL) alone (2), oxLDL plus 2.5 μg/mL Tan IIA (3), oxLDL plus 10 μg/mL Tan IIA (4) and oxLDL plus Cathepsin B inhibitor CA-074-Me (5). The Cathepsin B -/- mouse macrophages (6) were treated with oxLDL alone. The small interfering RNA-mediated CD36 silenced-mouse B6 macrophages (7) were treated with oxLDL alone. All the cells were stained with Alexa 647-conjugated cholera toxin B (membrane staining, red), and then with DQ ovalbumin (cathepsin B staining, green) and Hoechst dye (nuclei staining, blue) after saponin treatment. ( B ) Western blot detection for Cathepsin B releases from cytosol of oxLDL-induced B6 macrophages or cathepsin B -/- B6 macrophages treated with digitonin and phenylmethylsulfonyl fluoride (PMSF). ( C ) ELISA assay for oxLDL-induced Il-1β releases from mouse B6 macrophages or cathepsin B -/- B6 macrophages under the different treatments.

    Journal: Aging (Albany NY)

    Article Title: Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

    doi: 10.18632/aging.202202

    Figure Lengend Snippet: Tan IIA inhibits oxLDL-induced macrophage lysosomal damage and cathepsin B release. ( A ) Fluorescence confocal assay for oxLDL-induced cathepsin B release from lysosome of mouse B6 macrophages or cathepsin B -/- B6 macrophages under the different treatments, respectively. The cells were treated with oxLDL (50 μg/mL) alone (2), oxLDL plus 2.5 μg/mL Tan IIA (3), oxLDL plus 10 μg/mL Tan IIA (4) and oxLDL plus Cathepsin B inhibitor CA-074-Me (5). The Cathepsin B -/- mouse macrophages (6) were treated with oxLDL alone. The small interfering RNA-mediated CD36 silenced-mouse B6 macrophages (7) were treated with oxLDL alone. All the cells were stained with Alexa 647-conjugated cholera toxin B (membrane staining, red), and then with DQ ovalbumin (cathepsin B staining, green) and Hoechst dye (nuclei staining, blue) after saponin treatment. ( B ) Western blot detection for Cathepsin B releases from cytosol of oxLDL-induced B6 macrophages or cathepsin B -/- B6 macrophages treated with digitonin and phenylmethylsulfonyl fluoride (PMSF). ( C ) ELISA assay for oxLDL-induced Il-1β releases from mouse B6 macrophages or cathepsin B -/- B6 macrophages under the different treatments.

    Article Snippet: The measurements of all the above cytokines, except for IL-1β, were performed using commercially available ELISA kits (R & D Systems) according to the manufactures’ instructions.

    Techniques: Fluorescence, Confocal Assay, Small Interfering RNA, Staining, Western Blot, Enzyme-linked Immunosorbent Assay

    Effects of Tan IIA on HCh diet-induced AS lesion formation, serum lipid levels and cytokine productions in ApoE-/- mice. ( H – I ) Levels of LDL-Ch and TG in the sera of ApoE-deficient mice fed with different diets for 16 weeks. Oxidized LDL-induced and Tan IIA inhibited cytokine (IL-1β, TNFα, IL-6 and IL-18) productions at mRNA levels measured by qRT-PCR in the aortic tissues ( J – M ) and at protein levels determined by ELISA in the sera ( N – Q ). ( R , S ) Effects of Tan IIA (2.5, 5, 10 μg/mL) on oxLDL-induced IL-1β productions in the culture media of LPS-primed and un-primed mouse B6 macrophages, respectively. ( T , U ) Effects of Tan IIA on oxLDL-induced caspase-1 activation (10 kDa) and IL-1β maturation (17 kDa) in mouse B6 macrophages.

    Journal: Aging (Albany NY)

    Article Title: Tanshinone IIA attenuates atherosclerosis via inhibiting NLRP3 inflammasome activation

    doi: 10.18632/aging.202202

    Figure Lengend Snippet: Effects of Tan IIA on HCh diet-induced AS lesion formation, serum lipid levels and cytokine productions in ApoE-/- mice. ( H – I ) Levels of LDL-Ch and TG in the sera of ApoE-deficient mice fed with different diets for 16 weeks. Oxidized LDL-induced and Tan IIA inhibited cytokine (IL-1β, TNFα, IL-6 and IL-18) productions at mRNA levels measured by qRT-PCR in the aortic tissues ( J – M ) and at protein levels determined by ELISA in the sera ( N – Q ). ( R , S ) Effects of Tan IIA (2.5, 5, 10 μg/mL) on oxLDL-induced IL-1β productions in the culture media of LPS-primed and un-primed mouse B6 macrophages, respectively. ( T , U ) Effects of Tan IIA on oxLDL-induced caspase-1 activation (10 kDa) and IL-1β maturation (17 kDa) in mouse B6 macrophages.

    Article Snippet: The measurements of all the above cytokines, except for IL-1β, were performed using commercially available ELISA kits (R & D Systems) according to the manufactures’ instructions.

    Techniques: Mouse Assay, Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Activation Assay