electrospray ionization mass spectrometry esi-ms Search Results


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  • 99
    Millipore tmz
    Luciferase reporter assays for transcriptional activity from the TERT core promoter with −124 C > T or −146 C > T mutations compared to wild-type promoter in U87 cell lines. Cells were treated with <t>TMZ,</t> or cultured under hypoxic conditions through CoCl 2 treatment or exposure to 1% O 2 . WT, wild-type; 124T, −124 C > T mutation; 146T, −146 C > T mutation. (A) Compared to wild-type, 124T and 146T displayed significantly higher TERT promoter activity in the presence of TMZ. No significant difference was observed between the <t>DMSO</t> (control) and TMZ (50 µM) treatment groups. (B) Compared to wild-type, 124T and 146T displayed significantly higher TERT promoter activity under hypoxic conditions. No significant difference was observed between control and CoCl 2 treatment groups (B); similar results were observed when hypoxia was induced by exposure to 1% O 2 (C). The means of four measurements per experimental group are shown; error bars indicate standard deviation. * P
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    94
    Agilent technologies esi source
    <t>HPLC-electrospray</t> <t>(ESI)-negative</t> mass spectrum of metabolites of SKN dependent on NADPH, UDPGA, or both cofactors. Mass spectrum of SKN ( A ), mass spectrum of M1 ( B ), mass spectrum of M2 ( C ), mass spectrum of M3 ( D ), mass spectrum of M4 ( E ), mass spectrum of M5 ( F ), and mass spectrum of M6 ( G ) are shown. The metabolites are identified in Table 3 .
    Esi Source, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore guo
    The ESI+ mass spectra (recorded on fresh solutions and after 12 h) for the stability in methanol/water mixture, and for the interaction of the representative complex 2 with the mixture of cysteine and reduced glutathione (cys+GSH), and guanosine <t>5'-monophosphate</t> (GMP), given together with the spectra of the fresh solutions of the individual reactants, i.e. , the complex 2 , cys+GSH mixture and GMP.
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    94
    Thermo Fisher electrospray ionization source
    Δc hp1 transports SL 1278 to cell surface. <t>Electrospray</t> ionization Fourier transform ion cyclotron resonance MS analysis of lipid extracts from M. tuberculosis wild-type, Δ sap , Δ chp1 , and Δ mmpL8 strains showed that the three knock-out strains lack SL-1 and accumulate SL 1278 . (To aid comparison, the m / z 2460 ion belonging to the SL-1 series is marked with a plus sign , and the m / z 1277.9, 1278.9, and 1279.9 ions belonging to the SL 1278 series are marked with asterisks to distinguish them from other isobaric compounds in the lipid extracts). In addition, SL 1278 was found in the surface lipid fraction only in Δ chp1 , and both SL-1 production and the SL 1278 transport phenotype were restored by complementation with chp1 .
    Electrospray Ionization Source, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Agilent technologies jet stream esi source
    Δc hp1 transports SL 1278 to cell surface. <t>Electrospray</t> ionization Fourier transform ion cyclotron resonance MS analysis of lipid extracts from M. tuberculosis wild-type, Δ sap , Δ chp1 , and Δ mmpL8 strains showed that the three knock-out strains lack SL-1 and accumulate SL 1278 . (To aid comparison, the m / z 2460 ion belonging to the SL-1 series is marked with a plus sign , and the m / z 1277.9, 1278.9, and 1279.9 ions belonging to the SL 1278 series are marked with asterisks to distinguish them from other isobaric compounds in the lipid extracts). In addition, SL 1278 was found in the surface lipid fraction only in Δ chp1 , and both SL-1 production and the SL 1278 transport phenotype were restored by complementation with chp1 .
    Jet Stream Esi Source, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Waters Corporation electrospray ionization mass spectrometry
    Purification and identification of rLatroeggtoxin-V. ( A ) Chromatogram of RP-HPLC purification of rLatroeggtoxin-V. ( B ) Molecular weight determination of rLatroeggtoxin-V by <t>electrospray</t> ionization mass spectrometry. The m / z value and the number of charges for each rLatroeggtoxin-V ion peak were labeled.
    Electrospray Ionization Mass Spectrometry, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 246 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Waters Corporation electrospray ionization mass spectrometry esi ms
    <t>Electrospray</t> ionization-tandem mass spectrum of moanachelin gly-D (C14:0).
    Electrospray Ionization Mass Spectrometry Esi Ms, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 92/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation electrospray ionization
    6- O -Methylglucose lipopolysaccharide (mGLP), a derivative or similar product thereof, is responsible for expansion of mycobacterium inhibition-specific γ 9 δ 2 T cells. The 100% MeOH fraction eluted from silica was further analyzed by 1 H NMR and MALDI-TOF. (A) 1 H NMR analysis of the 100% MeOH eluate was performed on 4.0 mg of material. NMR spectra revealed a significant presence of O -methyl groups and α-anomeric protons corresponding to hexosyl residues. A representative 100% MeOH fraction (1 μl) from a silica gel column loaded with H37Rv total lipid from the chloroform-methanol-water (10:10:3) extraction and eluted with an increasing methanol gradient was mixed with DHB matrix (1 μl) and analyzed in negative <t>electrospray</t> mode. Spectra revealed a high-molecular-mass product in the m/z range of 3,600 to 4,000 (B), with peaks separated by 14 amu (C). (D) GC-MS profile of silica gel column fractions from the chloroform-methanol-water (10:10:3) extract: (1) neutral monosaccharide standard, (2) 100% MeOH, (3) 80% MeOH-CHCl 3 , (4) 60% MeOH-CHCl 3 , and (5) 40% MeOH-CHCl 3 .
    Electrospray Ionization, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1013 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore gm1 ganglioside
    Purification of three LT types and competitive binding assays carried out with the <t>GM1</t> ganglioside. (A) Representatives of LT1, LT2, and LT4 purified by galactose affinity chromatography and sorted in a Coomassie blue-stained polyacrylamide gel. Samples
    Gm1 Ganglioside, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Agilent technologies electrospray ionization mass spectrometry
    Analysis of purified recombinant cecropin AD by <t>electrospray</t> ionization mass spectrometry.
    Electrospray Ionization Mass Spectrometry, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 93/100, based on 315 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Agilent technologies g1607a ce esi ms sprayer kit
    Analysis of purified recombinant cecropin AD by <t>electrospray</t> ionization mass spectrometry.
    G1607a Ce Esi Ms Sprayer Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 91/100, based on 368 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Agilent technologies electrospray ionization mass spectrometry esi ms
    Analysis of purified recombinant cecropin AD by <t>electrospray</t> ionization mass spectrometry.
    Electrospray Ionization Mass Spectrometry Esi Ms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher electrospray ionization mass spectrometry
    Analysis of purified recombinant cecropin AD by <t>electrospray</t> ionization mass spectrometry.
    Electrospray Ionization Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 752 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    Millipore lutein
    Analysis of purified recombinant cecropin AD by <t>electrospray</t> ionization mass spectrometry.
    Lutein, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Luciferase reporter assays for transcriptional activity from the TERT core promoter with −124 C > T or −146 C > T mutations compared to wild-type promoter in U87 cell lines. Cells were treated with TMZ, or cultured under hypoxic conditions through CoCl 2 treatment or exposure to 1% O 2 . WT, wild-type; 124T, −124 C > T mutation; 146T, −146 C > T mutation. (A) Compared to wild-type, 124T and 146T displayed significantly higher TERT promoter activity in the presence of TMZ. No significant difference was observed between the DMSO (control) and TMZ (50 µM) treatment groups. (B) Compared to wild-type, 124T and 146T displayed significantly higher TERT promoter activity under hypoxic conditions. No significant difference was observed between control and CoCl 2 treatment groups (B); similar results were observed when hypoxia was induced by exposure to 1% O 2 (C). The means of four measurements per experimental group are shown; error bars indicate standard deviation. * P

    Journal: PLoS ONE

    Article Title: TERT Promoter Mutations Lead to High Transcriptional Activity under Hypoxia and Temozolomide Treatment and Predict Poor Prognosis in Gliomas

    doi: 10.1371/journal.pone.0100297

    Figure Lengend Snippet: Luciferase reporter assays for transcriptional activity from the TERT core promoter with −124 C > T or −146 C > T mutations compared to wild-type promoter in U87 cell lines. Cells were treated with TMZ, or cultured under hypoxic conditions through CoCl 2 treatment or exposure to 1% O 2 . WT, wild-type; 124T, −124 C > T mutation; 146T, −146 C > T mutation. (A) Compared to wild-type, 124T and 146T displayed significantly higher TERT promoter activity in the presence of TMZ. No significant difference was observed between the DMSO (control) and TMZ (50 µM) treatment groups. (B) Compared to wild-type, 124T and 146T displayed significantly higher TERT promoter activity under hypoxic conditions. No significant difference was observed between control and CoCl 2 treatment groups (B); similar results were observed when hypoxia was induced by exposure to 1% O 2 (C). The means of four measurements per experimental group are shown; error bars indicate standard deviation. * P

    Article Snippet: TMZ was dissolved in dimethyl sulfoxide (DMSO; Sigma-Aldrich, Saint Louis, USA); cells were treated with 50 µM TMZ for 24 h, at a final DMSO concentration of less than 0.1% (v/v).

    Techniques: Luciferase, Activity Assay, Cell Culture, Mutagenesis, Standard Deviation

    Pharmacological inhibition of Wnt/β-catenin signalling augments the cytotoxic effects of temozolomide. ( a ) Cell viability of cancer cell lines treated with increasing concentrations of different Wnt inhibitors and temozolomide (TMZ). Cell growth was assessed by FMCA or WST-1 after 72 h. All drugs were combined with a fixed molar ratio: TMZ:Celecoxib, 33:1; TMZ:G007-LK, 50:1; TMZ:LGK974, 20:1; TMZ:Wnt-C59, 20:1; TMZ:Salinomycin, 40:1; and TMZ:XAV-939, 20:1. Combination index (CI) at IC 70 was calculated by the median-effect method. Synergism and antagonism are defined as a CI mean significantly lower/higher than 1 with one-sample t -test ( P

    Journal: Nature Communications

    Article Title: Wnt/β-catenin pathway regulates MGMT gene expression in cancer and inhibition of Wnt signalling prevents chemoresistance

    doi: 10.1038/ncomms9904

    Figure Lengend Snippet: Pharmacological inhibition of Wnt/β-catenin signalling augments the cytotoxic effects of temozolomide. ( a ) Cell viability of cancer cell lines treated with increasing concentrations of different Wnt inhibitors and temozolomide (TMZ). Cell growth was assessed by FMCA or WST-1 after 72 h. All drugs were combined with a fixed molar ratio: TMZ:Celecoxib, 33:1; TMZ:G007-LK, 50:1; TMZ:LGK974, 20:1; TMZ:Wnt-C59, 20:1; TMZ:Salinomycin, 40:1; and TMZ:XAV-939, 20:1. Combination index (CI) at IC 70 was calculated by the median-effect method. Synergism and antagonism are defined as a CI mean significantly lower/higher than 1 with one-sample t -test ( P

    Article Snippet: Chemicals Celecoxib (Pfizer, Täby, Sweden), dimethyl-PGE2 (dmPGE2 ), cyclophosphamide (given as the active metabolite 4-hydroxycyclophosphamide), temozolomide, doxycycline, XAV-939, salinomycin (all from Sigma-Aldrich, Solna, Sweden), O6-BG, Wnt-C59 and LGK974 (Cayman Chemical, Ann Arbor, MI, USA) were dissolved in dimethyl sulfoxide (Sigma-Aldrich).

    Techniques: Inhibition

    Genetical inhibition of Wnt/β-catenin signalling augments the cytotoxic effects of temozolomide. ( a , b ) Inhibition of β-catenin expression by shRNA or siRNA sensitizes LS174T ( a ) and SW480 ( b ) colon carcinoma cells to temozolomide. LS174T cells were grown in medium containing ±1 mg ml −1 doxycycline (doxy) and treated with increasing concentrations of temozolomide (TMZ) twice (at 0 and 48 h) for totally 96 h. Doxy-induced shRNA knockdown of β-catenin expression significantly augment the cytotoxic effect of TMZ ( t -test on log IC 50 , P =0.0003). Overexpression of MGMT reversed the cytotoxic effect of TMZ caused by β-catenin knockdown ( t -test on log IC 50 , P =0.0491). Each concentration was tested in triplicate and the experiments were repeated twice. Values are mean±s.e.m. and presented as the percentage of matched control cells. Only depletion of β-catenin inhibited cell growth to 46±5.8% (mean±s.e.m.) of untreated control. ( b ) SW480 cells were transiently transfected with siRNA against β-catenin and subsequently treated with 100 μM TMZ for 48 h. Significant growth inhibition was observed in cells treated with a combination of siRNA against β-catenin and TMZ compared with only TMZ ( t -test, P =0.002). The experiment was repeated twice. Values are mean±s.e.m. ( c ) Clonogenic capacity of LS174T cells ±1 μg ml −1 doxy to induce shRNA against β-catenin and treated with 50 μM TMZ, as a single treatment or repeated treatment. The TMZ treatment was significantly more efficient in the shRNA-induced LS174T cells ( t -test, P =0.032). Each experimental point was performed in triplicate. The experiment was repeated with similar results. Mean±s.d. are displayed. ( d ) β-catenin knockdown significantly increases TMZ-induced apoptosis in LS174T cells. LS174T cells were incubated with ±1 μg ml −1 doxy to induce shRNA against β-catenin and treated with 50 μM TMZ for 48 h ( t -test, P =0.036). Apoptosis was analysed by flow cytometry measurement of cells in sub-G0 phase. The experiment was repeated three times. Values are mean±s.d.

    Journal: Nature Communications

    Article Title: Wnt/β-catenin pathway regulates MGMT gene expression in cancer and inhibition of Wnt signalling prevents chemoresistance

    doi: 10.1038/ncomms9904

    Figure Lengend Snippet: Genetical inhibition of Wnt/β-catenin signalling augments the cytotoxic effects of temozolomide. ( a , b ) Inhibition of β-catenin expression by shRNA or siRNA sensitizes LS174T ( a ) and SW480 ( b ) colon carcinoma cells to temozolomide. LS174T cells were grown in medium containing ±1 mg ml −1 doxycycline (doxy) and treated with increasing concentrations of temozolomide (TMZ) twice (at 0 and 48 h) for totally 96 h. Doxy-induced shRNA knockdown of β-catenin expression significantly augment the cytotoxic effect of TMZ ( t -test on log IC 50 , P =0.0003). Overexpression of MGMT reversed the cytotoxic effect of TMZ caused by β-catenin knockdown ( t -test on log IC 50 , P =0.0491). Each concentration was tested in triplicate and the experiments were repeated twice. Values are mean±s.e.m. and presented as the percentage of matched control cells. Only depletion of β-catenin inhibited cell growth to 46±5.8% (mean±s.e.m.) of untreated control. ( b ) SW480 cells were transiently transfected with siRNA against β-catenin and subsequently treated with 100 μM TMZ for 48 h. Significant growth inhibition was observed in cells treated with a combination of siRNA against β-catenin and TMZ compared with only TMZ ( t -test, P =0.002). The experiment was repeated twice. Values are mean±s.e.m. ( c ) Clonogenic capacity of LS174T cells ±1 μg ml −1 doxy to induce shRNA against β-catenin and treated with 50 μM TMZ, as a single treatment or repeated treatment. The TMZ treatment was significantly more efficient in the shRNA-induced LS174T cells ( t -test, P =0.032). Each experimental point was performed in triplicate. The experiment was repeated with similar results. Mean±s.d. are displayed. ( d ) β-catenin knockdown significantly increases TMZ-induced apoptosis in LS174T cells. LS174T cells were incubated with ±1 μg ml −1 doxy to induce shRNA against β-catenin and treated with 50 μM TMZ for 48 h ( t -test, P =0.036). Apoptosis was analysed by flow cytometry measurement of cells in sub-G0 phase. The experiment was repeated three times. Values are mean±s.d.

    Article Snippet: Chemicals Celecoxib (Pfizer, Täby, Sweden), dimethyl-PGE2 (dmPGE2 ), cyclophosphamide (given as the active metabolite 4-hydroxycyclophosphamide), temozolomide, doxycycline, XAV-939, salinomycin (all from Sigma-Aldrich, Solna, Sweden), O6-BG, Wnt-C59 and LGK974 (Cayman Chemical, Ann Arbor, MI, USA) were dissolved in dimethyl sulfoxide (Sigma-Aldrich).

    Techniques: Inhibition, Expressing, shRNA, Over Expression, Concentration Assay, Transfection, Incubation, Flow Cytometry, Cytometry

    Inhibition of Wnt/β-catenin in combination with temozolomide reduces tumour growth in vivo . ( a ) A combination of temozolomide and celecoxib significantly impairs the growth of established human medulloblastoma xenografts in NMRI nu/nu mice. Mice were engrafted with 7 × 10 6 D283 MED cells subcutaneously and randomized to receive either celecoxib (90 mg kg −1 ; n =12) through daily oral gastric feeding, temozolomide (7.5 mg kg −1 ; n =9; days 1–5), a combination of celecoxib and temozolomide ( n =9) or no treatment ( n =10), starting at the appearance of palpable tumours of approximately 0.10 ml (mean 0.13 ml). Celecoxib augments the inhibitory effect of temozolomide on medulloblastoma growth in vivo , as shown by the TVI (at day 12, celecoxib: TVI=6.2, P

    Journal: Nature Communications

    Article Title: Wnt/β-catenin pathway regulates MGMT gene expression in cancer and inhibition of Wnt signalling prevents chemoresistance

    doi: 10.1038/ncomms9904

    Figure Lengend Snippet: Inhibition of Wnt/β-catenin in combination with temozolomide reduces tumour growth in vivo . ( a ) A combination of temozolomide and celecoxib significantly impairs the growth of established human medulloblastoma xenografts in NMRI nu/nu mice. Mice were engrafted with 7 × 10 6 D283 MED cells subcutaneously and randomized to receive either celecoxib (90 mg kg −1 ; n =12) through daily oral gastric feeding, temozolomide (7.5 mg kg −1 ; n =9; days 1–5), a combination of celecoxib and temozolomide ( n =9) or no treatment ( n =10), starting at the appearance of palpable tumours of approximately 0.10 ml (mean 0.13 ml). Celecoxib augments the inhibitory effect of temozolomide on medulloblastoma growth in vivo , as shown by the TVI (at day 12, celecoxib: TVI=6.2, P

    Article Snippet: Chemicals Celecoxib (Pfizer, Täby, Sweden), dimethyl-PGE2 (dmPGE2 ), cyclophosphamide (given as the active metabolite 4-hydroxycyclophosphamide), temozolomide, doxycycline, XAV-939, salinomycin (all from Sigma-Aldrich, Solna, Sweden), O6-BG, Wnt-C59 and LGK974 (Cayman Chemical, Ann Arbor, MI, USA) were dissolved in dimethyl sulfoxide (Sigma-Aldrich).

    Techniques: Inhibition, In Vivo, Mouse Assay

    Pediatric brain tumors were more sensitive to carmofur than temozolomide Cell survival studies using MTT assays of various pediatric brain tumors are shown for control cells vs cells treated with 50 μM of carmofur and 100 μM of TMZ. Results are expressed as means and ± s.e.m ( N = 3).

    Journal: Oncotarget

    Article Title: Acid ceramidase is a novel drug target for pediatric brain tumors

    doi: 10.18632/oncotarget.15800

    Figure Lengend Snippet: Pediatric brain tumors were more sensitive to carmofur than temozolomide Cell survival studies using MTT assays of various pediatric brain tumors are shown for control cells vs cells treated with 50 μM of carmofur and 100 μM of TMZ. Results are expressed as means and ± s.e.m ( N = 3).

    Article Snippet: Anti-actin, carmofur, temozolomide (TMZ), and N-oleoylethanolamine (OE), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma Aldrich (St. Louis, MO).

    Techniques: MTT Assay

    HPLC-electrospray (ESI)-negative mass spectrum of metabolites of SKN dependent on NADPH, UDPGA, or both cofactors. Mass spectrum of SKN ( A ), mass spectrum of M1 ( B ), mass spectrum of M2 ( C ), mass spectrum of M3 ( D ), mass spectrum of M4 ( E ), mass spectrum of M5 ( F ), and mass spectrum of M6 ( G ) are shown. The metabolites are identified in Table 3 .

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Hepatic Metabolism of Sakuranetin and Its Modulating Effects on Cytochrome P450s and UDP-Glucuronosyltransferases

    doi: 10.3390/molecules23071542

    Figure Lengend Snippet: HPLC-electrospray (ESI)-negative mass spectrum of metabolites of SKN dependent on NADPH, UDPGA, or both cofactors. Mass spectrum of SKN ( A ), mass spectrum of M1 ( B ), mass spectrum of M2 ( C ), mass spectrum of M3 ( D ), mass spectrum of M4 ( E ), mass spectrum of M5 ( F ), and mass spectrum of M6 ( G ) are shown. The metabolites are identified in Table 3 .

    Article Snippet: LC-ESI-MS was performed with an LTQ linear ion trap mass spectrometer (Thermo Scientific, Waltham, MA, USA) with an ESI source, which was coupled with the Agilent HPLC system.

    Techniques: High Performance Liquid Chromatography

    Ion structures and their m / z values formed during mass spectrometry with electrospray ionization of BA-a benzoxazines obtained according to the Scheme 1 .: iminium ion derived from phenylaminomethylene fragment ( a ); fragments with one ( b , c ) and two ( d–f ) bisphenol moieties.

    Journal: Polymers

    Article Title: Synthesis of Phosphazene-Containing, Bisphenol A-Based Benzoxazines and Properties of Corresponding Polybenzoxazines

    doi: 10.3390/polym12061225

    Figure Lengend Snippet: Ion structures and their m / z values formed during mass spectrometry with electrospray ionization of BA-a benzoxazines obtained according to the Scheme 1 .: iminium ion derived from phenylaminomethylene fragment ( a ); fragments with one ( b , c ) and two ( d–f ) bisphenol moieties.

    Article Snippet: Liquid chromatography-mass spectrometry (LC-MS) with electrospray ionization (ESI+) was performed on an Agilent 1100 instrument (Agilent Technologies, Santa Clara, California, United States) using gradient elution in an acetonitrile-water system on a 4.6 mm Reprosil-Pur Basic C18 250 column with an ion trap LC-MSD-Trap-SL.

    Techniques: Mass Spectrometry, Derivative Assay

    The ESI+ mass spectra (recorded on fresh solutions and after 12 h) for the stability in methanol/water mixture, and for the interaction of the representative complex 2 with the mixture of cysteine and reduced glutathione (cys+GSH), and guanosine 5'-monophosphate (GMP), given together with the spectra of the fresh solutions of the individual reactants, i.e. , the complex 2 , cys+GSH mixture and GMP.

    Journal: Molecules

    Article Title: Synthesis, Characterization and in Vitro Antitumor Activity of Platinum(II) Oxalato Complexes Involving 7-Azaindole Derivatives as Coligands

    doi: 10.3390/molecules190810832

    Figure Lengend Snippet: The ESI+ mass spectra (recorded on fresh solutions and after 12 h) for the stability in methanol/water mixture, and for the interaction of the representative complex 2 with the mixture of cysteine and reduced glutathione (cys+GSH), and guanosine 5'-monophosphate (GMP), given together with the spectra of the fresh solutions of the individual reactants, i.e. , the complex 2 , cys+GSH mixture and GMP.

    Article Snippet: Materials and Methods Potassium tetrachloridoplatinate(II) (K2 [PtCl4 ]), potassium oxalate monohydrate (K2 (ox)∙H2 O), 4-chloro-7-azaindole (4Cl aza), 3-bromo-7-azaindole (3Br aza), 4-bromo-7-azaindole (4Br aza), cisplatin , oxaliplatin , cysteine (cys), reduced glutathione (GSH), guanosine 5'-monophosphate disodium salt (GMP) and solvents were purchased from Sigma-Aldrich Co. (Prague, Czech Republic) and Acros Organics Co. (Pardubice, Czech Republic) and used as received.

    Techniques:

    RASIP1 is a RAP1 effector that controls EC junction morphology. (A) Pull-down assay using purified RAP1A, RHOA, and RAC1 GST fusion proteins with control (c) or RASIP1 shRNA (kd) HUVEC lysates. RASIP1 interacts weakly with RAP1A-GST loaded with GDP, and

    Journal: Blood

    Article Title: Rasip1 regulates vertebrate vascular endothelial junction stability through Epac1-Rap1 signaling

    doi: 10.1182/blood-2013-02-483156

    Figure Lengend Snippet: RASIP1 is a RAP1 effector that controls EC junction morphology. (A) Pull-down assay using purified RAP1A, RHOA, and RAC1 GST fusion proteins with control (c) or RASIP1 shRNA (kd) HUVEC lysates. RASIP1 interacts weakly with RAP1A-GST loaded with GDP, and

    Article Snippet: Purified RAP1A–glutathione S-transferase (GST) (Sigma-Aldrich), Ras homolog gene family member A (RHOA)-GST, and RAC1A-GST (Novus) were incubated in cell lysis buffer with 10 μM guanosine diphosphate (GDP) or GTPγS (Millipore) for 30 minutes at 37°C.

    Techniques: Pull Down Assay, Purification, shRNA

    Δc hp1 transports SL 1278 to cell surface. Electrospray ionization Fourier transform ion cyclotron resonance MS analysis of lipid extracts from M. tuberculosis wild-type, Δ sap , Δ chp1 , and Δ mmpL8 strains showed that the three knock-out strains lack SL-1 and accumulate SL 1278 . (To aid comparison, the m / z 2460 ion belonging to the SL-1 series is marked with a plus sign , and the m / z 1277.9, 1278.9, and 1279.9 ions belonging to the SL 1278 series are marked with asterisks to distinguish them from other isobaric compounds in the lipid extracts). In addition, SL 1278 was found in the surface lipid fraction only in Δ chp1 , and both SL-1 production and the SL 1278 transport phenotype were restored by complementation with chp1 .

    Journal: The Journal of Biological Chemistry

    Article Title: Elucidation and Chemical Modulation of Sulfolipid-1 Biosynthesis in Mycobacterium tuberculosis *

    doi: 10.1074/jbc.M111.315473

    Figure Lengend Snippet: Δc hp1 transports SL 1278 to cell surface. Electrospray ionization Fourier transform ion cyclotron resonance MS analysis of lipid extracts from M. tuberculosis wild-type, Δ sap , Δ chp1 , and Δ mmpL8 strains showed that the three knock-out strains lack SL-1 and accumulate SL 1278 . (To aid comparison, the m / z 2460 ion belonging to the SL-1 series is marked with a plus sign , and the m / z 1277.9, 1278.9, and 1279.9 ions belonging to the SL 1278 series are marked with asterisks to distinguish them from other isobaric compounds in the lipid extracts). In addition, SL 1278 was found in the surface lipid fraction only in Δ chp1 , and both SL-1 production and the SL 1278 transport phenotype were restored by complementation with chp1 .

    Article Snippet: For the mass range m /z 300–1000, the capillary voltage was set to 4.5 kV, the capillary exit voltage was set to −300 V, the skimmer 1 voltage was set to −20 V, and the skimmer 2 voltage was set to −7 V. For the mass range m /z 1000–3000, the skimmer 2 voltage was lowered to approximately −1 to −3 V. Additional MSn spectra were obtained on an LTQ mass spectrometer equipped with an electrospray ionization source (Thermo Finnigan) operating in the negative ion mode.

    Techniques: Mass Spectrometry, Knock-Out

    ARDS plasma induces endothelial activation, which is attenuated by RTR ( A ) Plasma was collected from patients with ARDS and normal control, and plasma PGP and N-α-PGP levels were measured via electrospray ionization–liquid chromatography–tandem mass spectrometry. * P

    Journal: Science advances

    Article Title: The matrikine N-α-PGP couples extracellular matrix fragmentation to endothelial permeability

    doi: 10.1126/sciadv.1500175

    Figure Lengend Snippet: ARDS plasma induces endothelial activation, which is attenuated by RTR ( A ) Plasma was collected from patients with ARDS and normal control, and plasma PGP and N-α-PGP levels were measured via electrospray ionization–liquid chromatography–tandem mass spectrometry. * P

    Article Snippet: For plasma, a Finnigan TSQ Quantum Discovery Max triple quadrupole mass spectrometer was used with electrospray ionization (Thermo Fisher Scientific) on an Atlantis dC18 column [100 mm × 2.1 mm, particle diameter (dp) = 3 μm; Waters Chromatography] with an Atlantis dC18 precolumn (10 × 2.1 mm, dp = 3 μm; Waters), also coupled to a Shimadzu LC system.

    Techniques: Activation Assay, Liquid Chromatography, Mass Spectrometry

    Purification and identification of rLatroeggtoxin-V. ( A ) Chromatogram of RP-HPLC purification of rLatroeggtoxin-V. ( B ) Molecular weight determination of rLatroeggtoxin-V by electrospray ionization mass spectrometry. The m / z value and the number of charges for each rLatroeggtoxin-V ion peak were labeled.

    Journal: Toxins

    Article Title: Anti-Breast Cancer Activity of Latroeggtoxin-V Mined from the Transcriptome of Spider Latrodectus tredecimguttatus Eggs

    doi: 10.3390/toxins10110451

    Figure Lengend Snippet: Purification and identification of rLatroeggtoxin-V. ( A ) Chromatogram of RP-HPLC purification of rLatroeggtoxin-V. ( B ) Molecular weight determination of rLatroeggtoxin-V by electrospray ionization mass spectrometry. The m / z value and the number of charges for each rLatroeggtoxin-V ion peak were labeled.

    Article Snippet: The molecular weight of the components in each elution peak was determined by electrospray ionization mass spectrometry (Waters ACQUITY UPLC/Xevo G2 QTOF, Waters, Milford, MA, USA) and thus the peak containing recombinant Latroeggtoxin-V (rLatroeggtoxin-V) was selected and lyophilized.

    Techniques: Purification, High Performance Liquid Chromatography, Molecular Weight, Mass Spectrometry, Labeling

    Electrospray ionization-tandem mass spectrum of moanachelin gly-D (C14:0).

    Journal: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry

    Article Title: Amino acid variability in the peptide composition of a suite of amphiphilic peptide siderophores from an open ocean Vibrio species

    doi: 10.1007/s00775-013-0995-3

    Figure Lengend Snippet: Electrospray ionization-tandem mass spectrum of moanachelin gly-D (C14:0).

    Article Snippet: The masses of the siderophores and the siderophore fragments were determined by electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry on a Micromass Q-TOF2 (Waters Corp.).

    Techniques:

    Electrospray ionization-tandem mass spectrum of moanachelin ala-D (C14:0).

    Journal: Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry

    Article Title: Amino acid variability in the peptide composition of a suite of amphiphilic peptide siderophores from an open ocean Vibrio species

    doi: 10.1007/s00775-013-0995-3

    Figure Lengend Snippet: Electrospray ionization-tandem mass spectrum of moanachelin ala-D (C14:0).

    Article Snippet: The masses of the siderophores and the siderophore fragments were determined by electrospray ionization mass spectrometry (ESI-MS) and tandem mass spectrometry on a Micromass Q-TOF2 (Waters Corp.).

    Techniques:

    6- O -Methylglucose lipopolysaccharide (mGLP), a derivative or similar product thereof, is responsible for expansion of mycobacterium inhibition-specific γ 9 δ 2 T cells. The 100% MeOH fraction eluted from silica was further analyzed by 1 H NMR and MALDI-TOF. (A) 1 H NMR analysis of the 100% MeOH eluate was performed on 4.0 mg of material. NMR spectra revealed a significant presence of O -methyl groups and α-anomeric protons corresponding to hexosyl residues. A representative 100% MeOH fraction (1 μl) from a silica gel column loaded with H37Rv total lipid from the chloroform-methanol-water (10:10:3) extraction and eluted with an increasing methanol gradient was mixed with DHB matrix (1 μl) and analyzed in negative electrospray mode. Spectra revealed a high-molecular-mass product in the m/z range of 3,600 to 4,000 (B), with peaks separated by 14 amu (C). (D) GC-MS profile of silica gel column fractions from the chloroform-methanol-water (10:10:3) extract: (1) neutral monosaccharide standard, (2) 100% MeOH, (3) 80% MeOH-CHCl 3 , (4) 60% MeOH-CHCl 3 , and (5) 40% MeOH-CHCl 3 .

    Journal: Infection and Immunity

    Article Title: A Subset of Protective γ9δ2 T Cells Is Activated by Novel Mycobacterial Glycolipid Components

    doi: 10.1128/IAI.01322-15

    Figure Lengend Snippet: 6- O -Methylglucose lipopolysaccharide (mGLP), a derivative or similar product thereof, is responsible for expansion of mycobacterium inhibition-specific γ 9 δ 2 T cells. The 100% MeOH fraction eluted from silica was further analyzed by 1 H NMR and MALDI-TOF. (A) 1 H NMR analysis of the 100% MeOH eluate was performed on 4.0 mg of material. NMR spectra revealed a significant presence of O -methyl groups and α-anomeric protons corresponding to hexosyl residues. A representative 100% MeOH fraction (1 μl) from a silica gel column loaded with H37Rv total lipid from the chloroform-methanol-water (10:10:3) extraction and eluted with an increasing methanol gradient was mixed with DHB matrix (1 μl) and analyzed in negative electrospray mode. Spectra revealed a high-molecular-mass product in the m/z range of 3,600 to 4,000 (B), with peaks separated by 14 amu (C). (D) GC-MS profile of silica gel column fractions from the chloroform-methanol-water (10:10:3) extract: (1) neutral monosaccharide standard, (2) 100% MeOH, (3) 80% MeOH-CHCl 3 , (4) 60% MeOH-CHCl 3 , and (5) 40% MeOH-CHCl 3 .

    Article Snippet: A single-reaction monitoring (SRM) assay was developed for detection and quantification of HMBPP using a Waters Xevo TQ-S triple quadrapole mass spectrometer (MS) with electrospray ionization coupled to a Waters Acquity ultraperformance liquid chromatography instrument (UPLC).

    Techniques: Inhibition, Nuclear Magnetic Resonance, Gas Chromatography-Mass Spectrometry

    Purification of three LT types and competitive binding assays carried out with the GM1 ganglioside. (A) Representatives of LT1, LT2, and LT4 purified by galactose affinity chromatography and sorted in a Coomassie blue-stained polyacrylamide gel. Samples

    Journal: Journal of Bacteriology

    Article Title: Genetic Diversity of Heat-Labile Toxin Expressed by Enterotoxigenic Escherichia coli Strains Isolated from Humans

    doi: 10.1128/JB.00988-07

    Figure Lengend Snippet: Purification of three LT types and competitive binding assays carried out with the GM1 ganglioside. (A) Representatives of LT1, LT2, and LT4 purified by galactose affinity chromatography and sorted in a Coomassie blue-stained polyacrylamide gel. Samples

    Article Snippet: Briefly, wells of a polystyrene 96-well microtiter plate (Nalge Nunc) were coated with phosphate-buffered saline (PBS)-diluted GM1 ganglioside (0.05 μg ml−1 ; Sigma-Aldrich) and incubated overnight at room temperature.

    Techniques: Purification, Binding Assay, Affinity Chromatography, Staining

    (a) Predicted structure of vaccine constructs. Wild-type LT binds to GM1 receptors upon pentamerization of LT-B and translocates the toxic LT-A subunit into host cells. LTA2B-GH is predicted to pentamerize in a similar fashion to the wild-type toxin, thereby, presenting five copies of the G-H loop to the host. ntPE-LTA2B-GH additionally contains ntPE in place of LT-A1 in order to expand receptor repertoire of the vaccine. (b) Amino acid sequence of the “TCA” peptide. The N-terminus consists of a promiscuous T-helper cells epitope from VP4 of FMDV (bold), followed by linking amino acids GG (underlined), “site C” from VP1 (bold), another linker sequence (PPS, underlined), and site A (the G-H loop). (c) GM1 ELISA to illustrate the pentamerization of LTA2B-GH and ntPE-LTA2B-GH. Microtiter plates were coated with GM1 and incubated with CTB, TCA peptide, ntPE, LTA2B-GH, and ntPE-LTA2B-GH. Then they were tested with anti-G-H, anti-CTB and anti-ntPE antibodies to illustrate the GM1-binding capacity of fusion proteins. *** P value

    Journal: Advances in Virology

    Article Title: Bacterial Toxin Fusion Proteins Elicit Mucosal Immunity against a Foot-and-Mouth Disease Virus Antigen When Administered Intranasally to Guinea Pigs

    doi: 10.1155/2011/713769

    Figure Lengend Snippet: (a) Predicted structure of vaccine constructs. Wild-type LT binds to GM1 receptors upon pentamerization of LT-B and translocates the toxic LT-A subunit into host cells. LTA2B-GH is predicted to pentamerize in a similar fashion to the wild-type toxin, thereby, presenting five copies of the G-H loop to the host. ntPE-LTA2B-GH additionally contains ntPE in place of LT-A1 in order to expand receptor repertoire of the vaccine. (b) Amino acid sequence of the “TCA” peptide. The N-terminus consists of a promiscuous T-helper cells epitope from VP4 of FMDV (bold), followed by linking amino acids GG (underlined), “site C” from VP1 (bold), another linker sequence (PPS, underlined), and site A (the G-H loop). (c) GM1 ELISA to illustrate the pentamerization of LTA2B-GH and ntPE-LTA2B-GH. Microtiter plates were coated with GM1 and incubated with CTB, TCA peptide, ntPE, LTA2B-GH, and ntPE-LTA2B-GH. Then they were tested with anti-G-H, anti-CTB and anti-ntPE antibodies to illustrate the GM1-binding capacity of fusion proteins. *** P value

    Article Snippet: GM1 ELISA 96-well polystyrene Immulon-4 HBX microtiter plates (Dynex Technologies, Chantilly, VA) were coated with 50 μ L of 10 μ g/mL GM1 (monosialotetrahexosylganglioside, Sigma ) or BSA (negative control), diluted in coating buffer (0.05 M Na2 CO3 buffer, pH 9.6).

    Techniques: Construct, Sequencing, Enzyme-linked Immunosorbent Assay, Incubation, CtB Assay, Binding Assay

    PBA does not block the dissociation of CTA1 from CTA2/CTB 5 . After appending CT to a GM1-coated SPR sensor slide, a baseline measurement corresponding to the mass of the holotoxin was recorded. Reduced PDI was then perfused over the slide in the presence of 100 µM PBA. PDI was present in the perfusion buffer until approximately 500 sec into the experiment, after which it was replaced with sequential additions of an anti-PDI antibody, an anti-CTA antibody, and an anti-CTB antibody (arrowheads). One of two representative experiments is shown.

    Journal: PLoS ONE

    Article Title: A Therapeutic Chemical Chaperone Inhibits Cholera Intoxication and Unfolding/Translocation of the Cholera Toxin A1 Subunit

    doi: 10.1371/journal.pone.0018825

    Figure Lengend Snippet: PBA does not block the dissociation of CTA1 from CTA2/CTB 5 . After appending CT to a GM1-coated SPR sensor slide, a baseline measurement corresponding to the mass of the holotoxin was recorded. Reduced PDI was then perfused over the slide in the presence of 100 µM PBA. PDI was present in the perfusion buffer until approximately 500 sec into the experiment, after which it was replaced with sequential additions of an anti-PDI antibody, an anti-CTA antibody, and an anti-CTB antibody (arrowheads). One of two representative experiments is shown.

    Article Snippet: Materials The rabbit anti-CTA antibody, rabbit anti-CTB antibody, BfA, and ganglioside GM1 were purchased from Sigma-Aldrich (St. Louis, MO).

    Techniques: Blocking Assay, CtB Assay, SPR Assay, Size-exclusion Chromatography

    Analysis of purified recombinant cecropin AD by electrospray ionization mass spectrometry.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Novel Expression Vector for Secretion of Cecropin AD in Bacillus subtilis with Enhanced Antimicrobial Activity

    doi: 10.1128/AAC.00251-09

    Figure Lengend Snippet: Analysis of purified recombinant cecropin AD by electrospray ionization mass spectrometry.

    Article Snippet: Tricine-SDS-PAGE analysis of purified cecropin AD revealed that the molecular mass of cecropin AD was about 3.8 kDa, consistent with the theoretical molecular mass of 3.866 kDa obtained by electrospray ionization mass spectrometry.

    Techniques: Purification, Recombinant, Mass Spectrometry