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  • 93
    SCIEX electrospray ionization
    Increased CSF levels of ceramide and 4-hydroxynoneal (4-HNE) are associated with active dementia CSF levels of sphingolipids, sterols, triglyceride C52, reactive aldehyde, and tocopherol were determined by <t>electrospray</t> ionization tandem mass spectrometry (ESI/MS/MS). (A) Levels of sphingomyelin C16:0, C18:0, C20:0, C22:0, and C24:0 were increased in patients with inactive dementia (HIV-ID) compared with patients with no dementia (HIV-ND) or patients with active dementia (HIV-AD). (B) The ceramides C22:0, C24:0, and C24:1 and sulfatide were increased in HIV-AD patients compared with HIV-ND and HIV-ID patients. (C) Sphingomyelinase activity was increased in HIV-AD compared with HIV-ND or HIV-ID patients. (D) Levels of free cholesterol and the cholesterol esters C16:0 and C18:1 were similar in all HIV patient groups. (E) Levels of 25-hydroxycholesterol in CSF were decreased in HIV-AD patients compared with HIV-ND and HIV-ID groups. 25-OH = 25-hydroxy. (F) Triglyceride C52 levels were similar in all patient groups. (G) CSF levels of the reactive aldehydes 2-pentilpyrrole histidine-hydroxynoneal and 2-pentilpyrrole lysine-hydroxynoneal were increased in HIV-AD patients compared with HIV-ND and HIV-ID patients. (H) Vitamin E levels were decreased in patients with HIV-AD compared with HIV-ND and HIV-ID patient groups. ** p
    Electrospray Ionization, supplied by SCIEX, used in various techniques. Bioz Stars score: 93/100, based on 484 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Agilent technologies electrospray ionization
    Annotations of lipids by MS2Analyzer. (a) QTOF MS/MS of digalactosyldiacylglycerol 18:3/16:0 in positive <t>electrospray</t> mode, indicating the neutral loss of the DGDG headgroup from the [M + NH 4 ] + adduct precursor ion as well as m / z differences for product ions indicating both acyl side chains. Note that positional isomers of acyl groups cannot be determined with this method. (b) QTOF MS/MS of phosphatidylglycerol 16:0/16:0 in negative electrospray mode, indicating the characteristic product ions of the phosphatidylglycerol headgroup ( m / z 152.995) and the acyl side chains ( m / z 255.233).
    Electrospray Ionization, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 94/100, based on 1316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Waters Corporation electrospray ionization
    Isolation and identification of HEETA from the mouse aorta. Aortae were incubated with [ 14 C]AA in the presence of Indo (10 μM) and the presence and absence of the soluble epoxide hydrolase inhibitor 12-(3-adamantan-1-yl-ureido)dodecanoic acid (AUDA; 1 μM). A and B : samples were acidified in 25-fold excess of methanol, extracted, and analyzed by reverse-phase HPLC solvent system I . Migration times of known standards are indicated. C : methoxydihydroxyeicosatrienoic acid (MDHE) fractions were collected and analyzed by <t>electrospray</t> ionization with a Fourier transform ion cyclotron resonance MS. D : structure of MDHE with the major fragmentation site.
    Electrospray Ionization, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 94/100, based on 1125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher electrospray ionization
    ARDS plasma induces endothelial activation, which is attenuated by RTR ( A ) Plasma was collected from patients with ARDS and normal control, and plasma PGP and N-α-PGP levels were measured via <t>electrospray</t> ionization–liquid chromatography–tandem mass spectrometry. * P
    Electrospray Ionization, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 2601 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Shimadzu Corporation electrospray ionization
    Time-of-flight mass spectra of the disaccharides of HA/CS digested by HCLase. The main final products obtained from HA ( A ) and CS-A ( B ) digested by HCLase were identified by <t>electrospray</t> ionization MS on an ion trap TOF hybrid mass spectrometer as described under “Experimental Procedures.” Inten. , intensity.
    Electrospray Ionization, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 92/100, based on 83 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bruker Corporation electrospray ionization
    Experimental setup showing the arrangement of the <t>electrospray</t> ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate
    Electrospray Ionization, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 93/100, based on 445 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New Objective Inc electrospray ionization
    Experimental setup showing the arrangement of the <t>electrospray</t> ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate
    Electrospray Ionization, supplied by New Objective Inc, used in various techniques. Bioz Stars score: 92/100, based on 282 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    JEOL electrospray ionization
    Experimental setup showing the arrangement of the <t>electrospray</t> ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate
    Electrospray Ionization, supplied by JEOL, used in various techniques. Bioz Stars score: 92/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Micromass UK Limited electrospray ionization esi
    Experimental setup showing the arrangement of the <t>electrospray</t> ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate
    Electrospray Ionization Esi, supplied by Micromass UK Limited, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Bruker Corporation electrospray ionization probe
    Experimental setup showing the arrangement of the <t>electrospray</t> ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate
    Electrospray Ionization Probe, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Waters Corporation electrospray ionization probe
    Experimental setup showing the arrangement of the <t>electrospray</t> ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate
    Electrospray Ionization Probe, supplied by Waters Corporation, used in various techniques. Bioz Stars score: 89/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Shimadzu Corporation electrospray ionization probe
    Experimental setup showing the arrangement of the <t>electrospray</t> ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate
    Electrospray Ionization Probe, supplied by Shimadzu Corporation, used in various techniques. Bioz Stars score: 89/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Increased CSF levels of ceramide and 4-hydroxynoneal (4-HNE) are associated with active dementia CSF levels of sphingolipids, sterols, triglyceride C52, reactive aldehyde, and tocopherol were determined by electrospray ionization tandem mass spectrometry (ESI/MS/MS). (A) Levels of sphingomyelin C16:0, C18:0, C20:0, C22:0, and C24:0 were increased in patients with inactive dementia (HIV-ID) compared with patients with no dementia (HIV-ND) or patients with active dementia (HIV-AD). (B) The ceramides C22:0, C24:0, and C24:1 and sulfatide were increased in HIV-AD patients compared with HIV-ND and HIV-ID patients. (C) Sphingomyelinase activity was increased in HIV-AD compared with HIV-ND or HIV-ID patients. (D) Levels of free cholesterol and the cholesterol esters C16:0 and C18:1 were similar in all HIV patient groups. (E) Levels of 25-hydroxycholesterol in CSF were decreased in HIV-AD patients compared with HIV-ND and HIV-ID groups. 25-OH = 25-hydroxy. (F) Triglyceride C52 levels were similar in all patient groups. (G) CSF levels of the reactive aldehydes 2-pentilpyrrole histidine-hydroxynoneal and 2-pentilpyrrole lysine-hydroxynoneal were increased in HIV-AD patients compared with HIV-ND and HIV-ID patients. (H) Vitamin E levels were decreased in patients with HIV-AD compared with HIV-ND and HIV-ID patient groups. ** p

    Journal: Neurology

    Article Title: Associative and predictive biomarkers of dementia in HIV-1–infected patients

    doi: 10.1212/01.wnl.0000260610.79853.47

    Figure Lengend Snippet: Increased CSF levels of ceramide and 4-hydroxynoneal (4-HNE) are associated with active dementia CSF levels of sphingolipids, sterols, triglyceride C52, reactive aldehyde, and tocopherol were determined by electrospray ionization tandem mass spectrometry (ESI/MS/MS). (A) Levels of sphingomyelin C16:0, C18:0, C20:0, C22:0, and C24:0 were increased in patients with inactive dementia (HIV-ID) compared with patients with no dementia (HIV-ND) or patients with active dementia (HIV-AD). (B) The ceramides C22:0, C24:0, and C24:1 and sulfatide were increased in HIV-AD patients compared with HIV-ND and HIV-ID patients. (C) Sphingomyelinase activity was increased in HIV-AD compared with HIV-ND or HIV-ID patients. (D) Levels of free cholesterol and the cholesterol esters C16:0 and C18:1 were similar in all HIV patient groups. (E) Levels of 25-hydroxycholesterol in CSF were decreased in HIV-AD patients compared with HIV-ND and HIV-ID groups. 25-OH = 25-hydroxy. (F) Triglyceride C52 levels were similar in all patient groups. (G) CSF levels of the reactive aldehydes 2-pentilpyrrole histidine-hydroxynoneal and 2-pentilpyrrole lysine-hydroxynoneal were increased in HIV-AD patients compared with HIV-ND and HIV-ID patients. (H) Vitamin E levels were decreased in patients with HIV-AD compared with HIV-ND and HIV-ID patient groups. ** p

    Article Snippet: Electrospray ionization tandem mass-spectrometry (ESI/MS/MS) analyses were performed using methods similar to those used in our previous studies., Samples were injected using a Harvard Apparatus pump at 15 μ L/min into an electrospray ionization (i.e., Turbo Ion Spray module) Sciex API 3000 triple-stage quadruple tandem mass spectrometer from Sciex Inc. (Thornhill, Ontario, Canada) operated in the positive mode.

    Techniques: Mass Spectrometry, Activity Assay

    Elution profiles of m/z 732 in an untreated (blue trace) and m/z 650 in an ozonized (red trace) BAL sample in positive mode. The ozonized sample was exposed to 300 ppb ozone for 60 min. then subjected to LC-MS analysis (positive electrospray ionization)

    Journal: Analytical biochemistry

    Article Title: Identification of oxidized phospholipids in bronchoalveolar lavage exposed to low ozone levels using multivariate analysis

    doi: 10.1016/j.ab.2014.12.018

    Figure Lengend Snippet: Elution profiles of m/z 732 in an untreated (blue trace) and m/z 650 in an ozonized (red trace) BAL sample in positive mode. The ozonized sample was exposed to 300 ppb ozone for 60 min. then subjected to LC-MS analysis (positive electrospray ionization)

    Article Snippet: Reversed phase liquid chromatography (LC) and MS was performed on an AB Sciex API 3200 triple quadrupole mass spectrometer with an electrospray ionization source (AB Sciex, Concord, Canada).

    Techniques: Liquid Chromatography with Mass Spectroscopy

    Annotations of lipids by MS2Analyzer. (a) QTOF MS/MS of digalactosyldiacylglycerol 18:3/16:0 in positive electrospray mode, indicating the neutral loss of the DGDG headgroup from the [M + NH 4 ] + adduct precursor ion as well as m / z differences for product ions indicating both acyl side chains. Note that positional isomers of acyl groups cannot be determined with this method. (b) QTOF MS/MS of phosphatidylglycerol 16:0/16:0 in negative electrospray mode, indicating the characteristic product ions of the phosphatidylglycerol headgroup ( m / z 152.995) and the acyl side chains ( m / z 255.233).

    Journal: Analytical Chemistry

    Article Title: MS2Analyzer: A Software for Small Molecule Substructure Annotations from Accurate Tandem Mass Spectra

    doi: 10.1021/ac502818e

    Figure Lengend Snippet: Annotations of lipids by MS2Analyzer. (a) QTOF MS/MS of digalactosyldiacylglycerol 18:3/16:0 in positive electrospray mode, indicating the neutral loss of the DGDG headgroup from the [M + NH 4 ] + adduct precursor ion as well as m / z differences for product ions indicating both acyl side chains. Note that positional isomers of acyl groups cannot be determined with this method. (b) QTOF MS/MS of phosphatidylglycerol 16:0/16:0 in negative electrospray mode, indicating the characteristic product ions of the phosphatidylglycerol headgroup ( m / z 152.995) and the acyl side chains ( m / z 255.233).

    Article Snippet: In order to reduce the impact from different instruments, ionizations, and adducts, only [M + H]+ spectra acquired under positive electrospray ionization from Agilent QTOF 6530 mass spectrometers were used.

    Techniques: Mass Spectrometry

    HPLC-electrospray (ESI)-negative mass spectrum of metabolites of SKN dependent on NADPH, UDPGA, or both cofactors. Mass spectrum of SKN ( A ), mass spectrum of M1 ( B ), mass spectrum of M2 ( C ), mass spectrum of M3 ( D ), mass spectrum of M4 ( E ), mass spectrum of M5 ( F ), and mass spectrum of M6 ( G ) are shown. The metabolites are identified in Table 3 .

    Journal: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry

    Article Title: Hepatic Metabolism of Sakuranetin and Its Modulating Effects on Cytochrome P450s and UDP-Glucuronosyltransferases

    doi: 10.3390/molecules23071542

    Figure Lengend Snippet: HPLC-electrospray (ESI)-negative mass spectrum of metabolites of SKN dependent on NADPH, UDPGA, or both cofactors. Mass spectrum of SKN ( A ), mass spectrum of M1 ( B ), mass spectrum of M2 ( C ), mass spectrum of M3 ( D ), mass spectrum of M4 ( E ), mass spectrum of M5 ( F ), and mass spectrum of M6 ( G ) are shown. The metabolites are identified in Table 3 .

    Article Snippet: LC-ESI-MS was performed with an LTQ linear ion trap mass spectrometer (Thermo Scientific, Waltham, MA, USA) with an ESI source, which was coupled with the Agilent HPLC system.

    Techniques: High Performance Liquid Chromatography

    Isolation and identification of HEETA from the mouse aorta. Aortae were incubated with [ 14 C]AA in the presence of Indo (10 μM) and the presence and absence of the soluble epoxide hydrolase inhibitor 12-(3-adamantan-1-yl-ureido)dodecanoic acid (AUDA; 1 μM). A and B : samples were acidified in 25-fold excess of methanol, extracted, and analyzed by reverse-phase HPLC solvent system I . Migration times of known standards are indicated. C : methoxydihydroxyeicosatrienoic acid (MDHE) fractions were collected and analyzed by electrospray ionization with a Fourier transform ion cyclotron resonance MS. D : structure of MDHE with the major fragmentation site.

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    Article Title: Role of arachidonic acid lipoxygenase metabolites in acetylcholine-induced relaxations of mouse arteries

    doi: 10.1152/ajpheart.00696.2009

    Figure Lengend Snippet: Isolation and identification of HEETA from the mouse aorta. Aortae were incubated with [ 14 C]AA in the presence of Indo (10 μM) and the presence and absence of the soluble epoxide hydrolase inhibitor 12-(3-adamantan-1-yl-ureido)dodecanoic acid (AUDA; 1 μM). A and B : samples were acidified in 25-fold excess of methanol, extracted, and analyzed by reverse-phase HPLC solvent system I . Migration times of known standards are indicated. C : methoxydihydroxyeicosatrienoic acid (MDHE) fractions were collected and analyzed by electrospray ionization with a Fourier transform ion cyclotron resonance MS. D : structure of MDHE with the major fragmentation site.

    Article Snippet: THETA fractions were analyzed using electrospray ionization with a Fourier transform ion cyclotron resonance mass spectrometer (ESI-FT/MS, IonSpec 7.0-tesla FTICR high-resolution mass spectrometer with a Waters Z spray ESI source) ( ).

    Techniques: Isolation, Incubation, High Performance Liquid Chromatography, Migration, Mass Spectrometry

    Annotated mass spectral profile identifying green tea metabolites used in this study. (A) Positive electrospray ionization mode. (B) Negative electrospray ionization mode.

    Journal: Journal of Natural Products

    Article Title: Comparison of Metabolomics Approaches for Evaluating the Variability of Complex Botanical Preparations: Green Tea (Camellia sinensis) as a Case Study

    doi: 10.1021/acs.jnatprod.6b01156

    Figure Lengend Snippet: Annotated mass spectral profile identifying green tea metabolites used in this study. (A) Positive electrospray ionization mode. (B) Negative electrospray ionization mode.

    Article Snippet: Mass Spectrometry Analysis Ultraperformance (UP) LC-MS data were acquired using a Q Exactive Plus quadrupole-orbitrap mass spectrometer (Thermo Scientific, Waltham, MA, USA) with an electrospray ionization source coupled to an Acquity UPLC system (Waters, Milford, MA, USA).

    Techniques:

    ARDS plasma induces endothelial activation, which is attenuated by RTR ( A ) Plasma was collected from patients with ARDS and normal control, and plasma PGP and N-α-PGP levels were measured via electrospray ionization–liquid chromatography–tandem mass spectrometry. * P

    Journal: Science advances

    Article Title: The matrikine N-α-PGP couples extracellular matrix fragmentation to endothelial permeability

    doi: 10.1126/sciadv.1500175

    Figure Lengend Snippet: ARDS plasma induces endothelial activation, which is attenuated by RTR ( A ) Plasma was collected from patients with ARDS and normal control, and plasma PGP and N-α-PGP levels were measured via electrospray ionization–liquid chromatography–tandem mass spectrometry. * P

    Article Snippet: For plasma, a Finnigan TSQ Quantum Discovery Max triple quadrupole mass spectrometer was used with electrospray ionization (Thermo Fisher Scientific) on an Atlantis dC18 column [100 mm × 2.1 mm, particle diameter (dp) = 3 μm; Waters Chromatography] with an Atlantis dC18 precolumn (10 × 2.1 mm, dp = 3 μm; Waters), also coupled to a Shimadzu LC system.

    Techniques: Activation Assay, Liquid Chromatography, Mass Spectrometry

    Liquid chromatography negative electrospray ionisation tandem mass spectrometry analysis of serum, showing the m/z 464 MS2 data ( a ) eluting at 9.12 min ( b )

    Journal: Metabolomics

    Article Title: Serum metabolomics using ultra performance liquid chromatography coupled to mass spectrometry in lactating dairy cows following a single dose of sporidesmin

    doi: 10.1007/s11306-018-1358-4

    Figure Lengend Snippet: Liquid chromatography negative electrospray ionisation tandem mass spectrometry analysis of serum, showing the m/z 464 MS2 data ( a ) eluting at 9.12 min ( b )

    Article Snippet: The UPLC system consisted of an Accela 1250 quaternary UPLC pump, a PAL autosampler fitted with a 15,000 psi injection valve (CTC Analytics AG., Zwingen, Switzerland), a 20 µL injection loop, and a Q Exactive Orbitrap mass spectrometer with electrospray ionization source (Thermo Fisher Scientific, Waltham, MA, USA).

    Techniques: Liquid Chromatography, Mass Spectrometry

    Time-of-flight mass spectra of the disaccharides of HA/CS digested by HCLase. The main final products obtained from HA ( A ) and CS-A ( B ) digested by HCLase were identified by electrospray ionization MS on an ion trap TOF hybrid mass spectrometer as described under “Experimental Procedures.” Inten. , intensity.

    Journal: The Journal of Biological Chemistry

    Article Title: A Novel Eliminase from a Marine Bacterium That Degrades Hyaluronan and Chondroitin Sulfate *

    doi: 10.1074/jbc.M114.590752

    Figure Lengend Snippet: Time-of-flight mass spectra of the disaccharides of HA/CS digested by HCLase. The main final products obtained from HA ( A ) and CS-A ( B ) digested by HCLase were identified by electrospray ionization MS on an ion trap TOF hybrid mass spectrometer as described under “Experimental Procedures.” Inten. , intensity.

    Article Snippet: The major disaccharide fractions purified from HA and CS-A were further identified by electrospray ionization MS on an ion trap TOF hybrid mass spectrometer (LCMS-IT-TOF, Shimadzu, Japan).

    Techniques: Mass Spectrometry

    Experimental setup showing the arrangement of the electrospray ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate

    Journal: The Journal of Chemical Physics

    Article Title: Effective doping of low energy ions into superfluid helium droplets

    doi: 10.1063/1.4928689

    Figure Lengend Snippet: Experimental setup showing the arrangement of the electrospray ionization source, the superfluid helium droplet source, and the analysis region of the doped droplets including the Daly dynode detector. The cesium source was temporarily used to calibrate

    Article Snippet: The ion source was adapted from a reflectron mass spectrometer with electrospray ionization (Bruker Daltonics, BioTOF), and the reflectron component was removed for this experiment.

    Techniques: