Journal: Journal of the American Society for Mass Spectrometry
Article Title: Monitoring Copopulated Conformational States During Protein Folding Events Using Electrospray Ionization-Ion Mobility Spectrometry-Mass Spectrometry
Figure Lengend Snippet: ( a )–( c ): ESI-IMS-MS Driftscope plots showing drift time (x axis) versus m/z (y axis) for β 2 m and variants at pH 6.8. ( a ) wild-type β 2 m, ( b ) single mutant I7A, and ( c ) double mutant I7A/P32G. Insets at the right hand side: the summed, full scan m/z spectra of each protein showing the charge state ions detected.
Article Snippet: ESI-IMS-MS Analyses Samples were infused into the ESI source of a Synapt HDMS, a hybrid quadrupole-IMS-orthogonal acceleration time-of-flight mass spectrometer (oa-TOF) [ ] (Waters UK Ltd, Manchester, UK) using a Harvard syringe pump (model 22; Harvard Apparatus, Holliston, MA) with a flow rate of 10 μL min−1 .
Techniques: Mass Spectrometry, Mutagenesis