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  • 79
    Bio-Rad electroporation cuvettes bio rad
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Electroporation Cuvettes Bio Rad, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 79/100, based on 598 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 598 article reviews
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    99
    Bio-Rad electroporation cuvettes
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Electroporation Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 858 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    82
    Bio-Rad electroporation cuvet
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Electroporation Cuvet, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 82/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 18 article reviews
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    electroporation cuvet - by Bioz Stars, 2020-01
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    93
    Bio-Rad bio rad cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Bio Rad Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 41 article reviews
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    90
    Bio-Rad bio rad cuvettes
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Bio Rad Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 46 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 46 article reviews
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    99
    Bio-Rad electroporation
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Electroporation, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 11266 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Bio-Rad generic electroporation cuvettes
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Generic Electroporation Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Bio-Rad wide electroporation cuvettes
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Wide Electroporation Cuvettes, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    Bio-Rad square wave electroporation
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Square Wave Electroporation, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Bio-Rad sterile electroporation cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Sterile Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Bio-Rad genepulser electroporation cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Genepulser Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Bio-Rad cold electroporation cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Cold Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Bio-Rad gene pulser electroporation cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Gene Pulser Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 86/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    78
    Bio-Rad electrode gap electroporation cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Electrode Gap Electroporation Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 78/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    70
    Bio-Rad 1 mm path length electroporation cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad bio rad gene pulser cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad electroporator
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad electroporator chamber
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad gene pulser xcell electroporation system
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad gene pulser xcell microbial electroporation system
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad gene pulser electroporator ii
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad protein assay kit
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Bio-Rad cuvette chamber
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    Cuvette Chamber, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 81/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Bio-Rad 0 4 mm cuvette
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
    0 4 Mm Cuvette, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 80/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad genepulser xcell
    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to <t>electroporation</t> number/colony number.
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    Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to electroporation number/colony number.

    Journal: Molecular Plant Pathology

    Article Title: Pectate lyase affects pathogenicity in natural isolates of Colletotrichum coccodes and in pelA gene‐disrupted and gene‐overexpressing mutant lines

    doi: 10.1111/j.1364-3703.2011.00740.x

    Figure Lengend Snippet: Targeted gene disruption of CcpelA . (A–C) Schematic diagram of the strategy used for targeted gene disruption of CcpelA encoding pectate lyase (PL). (A) CcpelA in wild‐type (WT) genome. (B) Linearized pGH1‐1240PL plasmid containing CcpelA sequence. (C) Genome organization of CcpelA disrupted via homologous recombination. Box colours: dark and light grey, CcpelA gene sequence (sequence marked in dark grey was included in the disruption construct); black, sequences derived from pGEM vector; white shading, hygromycin resistance gene; white, other vector sequences. Arrows indicate the positions and orientations of the polymerase chain reaction (PCR) primers used for verification of the transformants: 1, For‐new‐PL4; 2, Rev‐new‐PL4; 3, For‐pGEM; 4, Rev‐pGEM. (D–F) PCR analysis of CcpelA gene‐disrupted transformants. Genomic DNA isolated from wild‐type (WT) isolates US‐41 and Si‐72, CcpelA gene‐disrupted transformants US‐41/7.1 and Si‐72/2.1, and representatives of ectopic transformants US‐41/5.1 and Si‐72/5.1 were analysed using the following primers sets: (D) For‐new‐PL4 and Rev‐new‐PL4 (1 and 2 in A and C); (E) For‐new‐PL4 and For‐pGEM (1 and 3 in C); (F) Rev‐new‐PL4 and Rev‐pGEM (2 and 4 in C). All PCR products were run on a 1% agarose gel and stained with ethidium bromide. Mutants were numbered according to electroporation number/colony number.

    Article Snippet: Aliquots (0.1 mL) were placed into cold 0.2‐cm electroporation cuvettes (Bio‐Rad) and linearized DNA plasmid (5–10 µg) was added.

    Techniques: Plasmid Preparation, Sequencing, Homologous Recombination, Construct, Derivative Assay, Polymerase Chain Reaction, Isolation, Agarose Gel Electrophoresis, Staining, Electroporation

    GR site - specific phosphorylation , auto - induction , and potency in Dex - sensitive and – resistant CEM cells. A . GR ser211 phosphorylation and auto-induction. Naturally Dex-sensitive CEM-C7-14 (C7-14), Dex-resistant CEM-C1-15 (C1-15), and two resistant-to-sensitive revertant clones; CEM-C1-6 (spontaneous revertant, C1-6), and CEM-IV B9 (demethylated revertant, IV B9) were treated in triplicate for 16 hours with 100 nM Dex (D) or an equivalent volume of ethanol vehicle (C). Cell lysates were then analyzed for phospho-GR S211, total GR, and actin by immunoblot. Depicted is a representative blot from 3 independent samples done, all with nearly identical results. n = 3. The data shown are from a single gel, with irrelevant sections removed, for clarity. B . Dex/GR-dependent transcriptional activity. CEM; C1-15, C1-6, and IV B9 cells were transfected by electroporation with a GRE- dependent luciferase reporter plasmid. Cells were then divided into triplicate replicates and exposed to either ethanol vehicle (control) or 1 μM Dex for 24 hours after which time extracts were made and luciferase activity analyzed. A representative experiment is shown of average RLUs normalized to μg of protein in the cellular lysate. Error bars = 1 standard deviation from the mean. Total biological replicates, n = 2 for C1-15; 5 for C1-6; and 7 for IV B9.

    Journal: Cancer Cell International

    Article Title: Epigenetic alteration by DNA-demethylating treatment restores apoptotic response to glucocorticoids in dexamethasone-resistant human malignant lymphoid cells

    doi: 10.1186/1475-2867-14-35

    Figure Lengend Snippet: GR site - specific phosphorylation , auto - induction , and potency in Dex - sensitive and – resistant CEM cells. A . GR ser211 phosphorylation and auto-induction. Naturally Dex-sensitive CEM-C7-14 (C7-14), Dex-resistant CEM-C1-15 (C1-15), and two resistant-to-sensitive revertant clones; CEM-C1-6 (spontaneous revertant, C1-6), and CEM-IV B9 (demethylated revertant, IV B9) were treated in triplicate for 16 hours with 100 nM Dex (D) or an equivalent volume of ethanol vehicle (C). Cell lysates were then analyzed for phospho-GR S211, total GR, and actin by immunoblot. Depicted is a representative blot from 3 independent samples done, all with nearly identical results. n = 3. The data shown are from a single gel, with irrelevant sections removed, for clarity. B . Dex/GR-dependent transcriptional activity. CEM; C1-15, C1-6, and IV B9 cells were transfected by electroporation with a GRE- dependent luciferase reporter plasmid. Cells were then divided into triplicate replicates and exposed to either ethanol vehicle (control) or 1 μM Dex for 24 hours after which time extracts were made and luciferase activity analyzed. A representative experiment is shown of average RLUs normalized to μg of protein in the cellular lysate. Error bars = 1 standard deviation from the mean. Total biological replicates, n = 2 for C1-15; 5 for C1-6; and 7 for IV B9.

    Article Snippet: Aliquots (400 μl) of the suspension were placed into 0.4 cm gap electroporation cuvettes (Bio-Rad, Hercules, CA) containing 15 μg of GRE-dependant mouse mammary tumor virus (MMTV) luciferase reporter vector phh-Luc (ATCC) prepared using a Qiagen maxi-prep kit (Qiagen).

    Techniques: Clone Assay, Activity Assay, Transfection, Electroporation, Luciferase, Plasmid Preparation, Standard Deviation

    Fluorescent microscopy analysis of the transient permeabilization of BY-2 cells by electroporation with the lipophilic styryl fluorescent dye FM and ERD14-C186-Alexa Fluor 647 . Normal BY-2 cells before being electroporated (A) are not permeable to FM (green). BY-2 cells in which the plasma membrane is disrupted by electroporation (B–D) display incorporation of FM that is mainly located at the nucleus. ERD14-C186-Alexa Fluor 647 penetrates the inner space of BY-2 cells after electroporation (E–G) .

    Journal: Frontiers in Plant Science

    Article Title: Protein Delivery into Plant Cells: Toward In vivo Structural Biology

    doi: 10.3389/fpls.2017.00519

    Figure Lengend Snippet: Fluorescent microscopy analysis of the transient permeabilization of BY-2 cells by electroporation with the lipophilic styryl fluorescent dye FM and ERD14-C186-Alexa Fluor 647 . Normal BY-2 cells before being electroporated (A) are not permeable to FM (green). BY-2 cells in which the plasma membrane is disrupted by electroporation (B–D) display incorporation of FM that is mainly located at the nucleus. ERD14-C186-Alexa Fluor 647 penetrates the inner space of BY-2 cells after electroporation (E–G) .

    Article Snippet: Transformation of agrobacterium tumefaciens with the appropriate DNA constructs of ERD14/10 An aliquot of electrocompetent A. tumefaciens LBA4404 cells (50 μL) was transferred into a pre-cooled electroporation cuvette (Bio-Rad Laboratories Inc., Cat No. 165-2086) and mixed with 2 μL of DNA (pK7WGR2.0-ERD14-RFP or pK7WGR2.0-ERD10-RFP) ensuring that no air bubbles were present.

    Techniques: Microscopy, Electroporation