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  • 94
    Millipore derivatization reagents methyl chloroformate mcf
    Derivatization Reagents Methyl Chloroformate Mcf, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/derivatization reagents methyl chloroformate mcf/product/Millipore
    Average 94 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    derivatization reagents methyl chloroformate mcf - by Bioz Stars, 2020-08
    94/100 stars
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    85
    BOC Sciences electrophilic reagent
    Electrophilic Reagent, supplied by BOC Sciences, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrophilic reagent/product/BOC Sciences
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    electrophilic reagent - by Bioz Stars, 2020-08
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    90
    Xenobiotics electrophiles
    Electrophiles, supplied by Xenobiotics, used in various techniques. Bioz Stars score: 90/100, based on 274 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrophiles/product/Xenobiotics
    Average 90 stars, based on 274 article reviews
    Price from $9.99 to $1999.99
    electrophiles - by Bioz Stars, 2020-08
    90/100 stars
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    85
    Millipore electrophilic reagent 7 chloro 4 nitrobenzo 2 oxa 1
    Electrophilic Reagent 7 Chloro 4 Nitrobenzo 2 Oxa 1, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/electrophilic reagent 7 chloro 4 nitrobenzo 2 oxa 1/product/Millipore
    Average 85 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    electrophilic reagent 7 chloro 4 nitrobenzo 2 oxa 1 - by Bioz Stars, 2020-08
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    94
    Millipore lithium aluminum hydride powder
    Lithium Aluminum Hydride Powder, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lithium aluminum hydride powder/product/Millipore
    Average 94 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    lithium aluminum hydride powder - by Bioz Stars, 2020-08
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    93
    Millipore mbth
    Mbth, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mbth/product/Millipore
    Average 93 stars, based on 44 article reviews
    Price from $9.99 to $1999.99
    mbth - by Bioz Stars, 2020-08
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    99
    Millipore 10 phenanthroline phenanthroline
    Uni- and bi-dimensional electrophoretic analysis of B. lanceolatus venom, its cross-reaction with bothropic antivenom, and the presence of glycosylated proteins in the venom. ( A , C , D ) Samples of B. lanceolatus venom were submitted to electrophoretic SDS-PAGE separation (12% of acrylamide) in non-reducing ( a ) and reducing ( b ) conditions. ( A ) The gel on which 30 µg of venom was separated was stained with Coomassie Blue R-250. ( B ) Strips with pH gradient (3 to 10) were rehydrated with buffer containing B. lanceolatus venom (100 µg) before isofocalization. After washing with reducing and chelating buffers, the focalized strips were submitted to SDS-PAGE electrophoresis (12% of acrylamide). The resulting gels were silver stained. ( C , D ) Venom samples (15 µg) were separated by electrophoresis and electrotransferred to nitrocellulose membranes. The membranes were incubated with the peroxidase-conjugated lectins, WGA and Con A. Recognized bands were visualized with DAB. ( E ) After electrophoresis in non-reducing conditions, samples (5 µg) of B. jararaca (Bj) and B. lanceolatus (Bl) venoms were electrotransferred to nitrocellulose membranes and incubated with bothropic antivenom diluted <t>1:10,000</t> followed by GAH/IgG-AP (1:7500). Cross-reacting bands were visualized with NBT-BCIP. ( F ) ELISA plates were coated with B. lanceolatus venom (1 µg/well). They were incubated with serial dilutions of bothropic antivenom or botulinum toxin antiserum, as negative control, followed by GAH/IgG-AP (1:3000). The results showed are representative of two experiments, realized in duplicates. The titer was determined as the highest antivenom dilution, which produced an absorbance eight times greater than the absorbance determined for the control serum. This absorbance value is represented by a dotted line.
    10 Phenanthroline Phenanthroline, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/10 phenanthroline phenanthroline/product/Millipore
    Average 99 stars, based on 95 article reviews
    Price from $9.99 to $1999.99
    10 phenanthroline phenanthroline - by Bioz Stars, 2020-08
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    Image Search Results


    Uni- and bi-dimensional electrophoretic analysis of B. lanceolatus venom, its cross-reaction with bothropic antivenom, and the presence of glycosylated proteins in the venom. ( A , C , D ) Samples of B. lanceolatus venom were submitted to electrophoretic SDS-PAGE separation (12% of acrylamide) in non-reducing ( a ) and reducing ( b ) conditions. ( A ) The gel on which 30 µg of venom was separated was stained with Coomassie Blue R-250. ( B ) Strips with pH gradient (3 to 10) were rehydrated with buffer containing B. lanceolatus venom (100 µg) before isofocalization. After washing with reducing and chelating buffers, the focalized strips were submitted to SDS-PAGE electrophoresis (12% of acrylamide). The resulting gels were silver stained. ( C , D ) Venom samples (15 µg) were separated by electrophoresis and electrotransferred to nitrocellulose membranes. The membranes were incubated with the peroxidase-conjugated lectins, WGA and Con A. Recognized bands were visualized with DAB. ( E ) After electrophoresis in non-reducing conditions, samples (5 µg) of B. jararaca (Bj) and B. lanceolatus (Bl) venoms were electrotransferred to nitrocellulose membranes and incubated with bothropic antivenom diluted 1:10,000 followed by GAH/IgG-AP (1:7500). Cross-reacting bands were visualized with NBT-BCIP. ( F ) ELISA plates were coated with B. lanceolatus venom (1 µg/well). They were incubated with serial dilutions of bothropic antivenom or botulinum toxin antiserum, as negative control, followed by GAH/IgG-AP (1:3000). The results showed are representative of two experiments, realized in duplicates. The titer was determined as the highest antivenom dilution, which produced an absorbance eight times greater than the absorbance determined for the control serum. This absorbance value is represented by a dotted line.

    Journal: Toxins

    Article Title: Enzymatic and Pro-Inflammatory Activities of Bothrops lanceolatus Venom: Relevance for Envenomation

    doi: 10.3390/toxins9080244

    Figure Lengend Snippet: Uni- and bi-dimensional electrophoretic analysis of B. lanceolatus venom, its cross-reaction with bothropic antivenom, and the presence of glycosylated proteins in the venom. ( A , C , D ) Samples of B. lanceolatus venom were submitted to electrophoretic SDS-PAGE separation (12% of acrylamide) in non-reducing ( a ) and reducing ( b ) conditions. ( A ) The gel on which 30 µg of venom was separated was stained with Coomassie Blue R-250. ( B ) Strips with pH gradient (3 to 10) were rehydrated with buffer containing B. lanceolatus venom (100 µg) before isofocalization. After washing with reducing and chelating buffers, the focalized strips were submitted to SDS-PAGE electrophoresis (12% of acrylamide). The resulting gels were silver stained. ( C , D ) Venom samples (15 µg) were separated by electrophoresis and electrotransferred to nitrocellulose membranes. The membranes were incubated with the peroxidase-conjugated lectins, WGA and Con A. Recognized bands were visualized with DAB. ( E ) After electrophoresis in non-reducing conditions, samples (5 µg) of B. jararaca (Bj) and B. lanceolatus (Bl) venoms were electrotransferred to nitrocellulose membranes and incubated with bothropic antivenom diluted 1:10,000 followed by GAH/IgG-AP (1:7500). Cross-reacting bands were visualized with NBT-BCIP. ( F ) ELISA plates were coated with B. lanceolatus venom (1 µg/well). They were incubated with serial dilutions of bothropic antivenom or botulinum toxin antiserum, as negative control, followed by GAH/IgG-AP (1:3000). The results showed are representative of two experiments, realized in duplicates. The titer was determined as the highest antivenom dilution, which produced an absorbance eight times greater than the absorbance determined for the control serum. This absorbance value is represented by a dotted line.

    Article Snippet: Chemicals and Reagents Triton X-100, Tween 20, Brij-35, EDTA, N -Cetyl-N ,N ,N -trimethylammonium bromide (CTAB), ortho-phenylenediamine (OPD), Coomassie Blue R-250, hyaluronic acid, diaminobenzidine (DAB), phenylmethanesulfonyl fluoride (PMSF), 1,10-phenanthroline, bovine serum albumin (BSA), fibrinogen from human plasma, gelatin type A, goat anti-horse (GAH) IgG horseradish peroxidase (IgGHRPO), concanavalin A (Con A), and Wheat Germ Agglutinin (WGA) labelled with peroxidase, were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: SDS Page, Staining, Electrophoresis, Incubation, Whole Genome Amplification, Enzyme-linked Immunosorbent Assay, Negative Control, Produced

    Gelatinolytic and fibrinogenolytic activities of B. lanceolatus venom. ( A , B ) Samples of B. lanceolatus venom (25 µg) were separated by SDS-PAGE in 12% acrylamide gels containing ( A ) 10% gelatin or ( B ) 10% fibrinogen under non-reducing conditions at 4 °C. The gels were then incubated overnight at 37 °C in substrate buffer (pH 8.3) and stained with Coomassie Blue R250. ( C ) Samples of fibrinogen (30 µg) were incubated with B. lanceolatus venom (0.5 µg) for 1 h at 37 °C in the absence or presence of proteases inhibitors (20 mM), EDTA, 1,10-phenanthroline (Phen), or PMSF. Samples were then separated by SDS-PAGE electrophoresis in reducing conditions before Coomassie Blue R250 staining.

    Journal: Toxins

    Article Title: Enzymatic and Pro-Inflammatory Activities of Bothrops lanceolatus Venom: Relevance for Envenomation

    doi: 10.3390/toxins9080244

    Figure Lengend Snippet: Gelatinolytic and fibrinogenolytic activities of B. lanceolatus venom. ( A , B ) Samples of B. lanceolatus venom (25 µg) were separated by SDS-PAGE in 12% acrylamide gels containing ( A ) 10% gelatin or ( B ) 10% fibrinogen under non-reducing conditions at 4 °C. The gels were then incubated overnight at 37 °C in substrate buffer (pH 8.3) and stained with Coomassie Blue R250. ( C ) Samples of fibrinogen (30 µg) were incubated with B. lanceolatus venom (0.5 µg) for 1 h at 37 °C in the absence or presence of proteases inhibitors (20 mM), EDTA, 1,10-phenanthroline (Phen), or PMSF. Samples were then separated by SDS-PAGE electrophoresis in reducing conditions before Coomassie Blue R250 staining.

    Article Snippet: Chemicals and Reagents Triton X-100, Tween 20, Brij-35, EDTA, N -Cetyl-N ,N ,N -trimethylammonium bromide (CTAB), ortho-phenylenediamine (OPD), Coomassie Blue R-250, hyaluronic acid, diaminobenzidine (DAB), phenylmethanesulfonyl fluoride (PMSF), 1,10-phenanthroline, bovine serum albumin (BSA), fibrinogen from human plasma, gelatin type A, goat anti-horse (GAH) IgG horseradish peroxidase (IgGHRPO), concanavalin A (Con A), and Wheat Germ Agglutinin (WGA) labelled with peroxidase, were purchased from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: SDS Page, Incubation, Staining, Electrophoresis