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  • 90
    Thermo Fisher tetra acetoxymethyl ester
    Tetra Acetoxymethyl Ester, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tetra acetoxymethyl ester/product/Thermo Fisher
    Average 90 stars, based on 7 article reviews
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    tetra acetoxymethyl ester - by Bioz Stars, 2020-02
    90/100 stars
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    N/A
    Infuse only dual syringe pump Accomodates syringes 0 5 muL to 10 mL User definable flow rates with selectable target volume or time values to control the total infusion volume
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    96
    Boston BioProducts egta
    Egta, supplied by Boston BioProducts, used in various techniques. Bioz Stars score: 96/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Boston BioProducts
    Average 96 stars, based on 155 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    96
    Carl Roth GmbH egta
    Effect of PF-IgGs on binding of Dsg1-coated beads to <t>HaCaT</t> cells. Beads were allowed to settle on the surface of HaCaT cells for 30 minutes (control). Number of bound beads was reduced by simultaneous incubation of <t>EGTA</t> (5 mM, 30 minutes). Incubation of monolayers with attached beads for an additional 30 minutes with IgG fractions from 2 patients (PF1- and PF2-IgG, 35 μg/ml each) as well as with a monoclonal antibody (1:50) directed against the extracellular domain of human Dsg1 significantly reduced the number of bound beads (label in white box and white bars). Immunoabsorption using Dsg1-coated beads but not control absorption using VE-cadherin–labeled beads completely abolished the effect of PF-IgGs on bead adhesion. Preincubation of HaCaT cells with PF-IgGs prior to bead settlement also reduced bead binding (label in gray box and gray bars) whereas preincubation of beads with PF-IgGs did not inhibit bead binding. In contrast, the monoclonal Dsg1 antibody reduced bead binding also when applied for preincubation with beads, indicating a different mechanism underlying the reduction of Dsg1 adhesion ( n = 6 for each condition).
    Egta, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 96/100, based on 107 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 107 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    94
    Dojindo Labs egta
    ATP's antimicrobial effect is attributable to its iron-chelating activity. (a) Blocking effects of MgCl 2 and FeCl 3 on ATP's anti- M. intracellulare antimicrobial activity. (b) Anti- M. intracellulare activities of ATP and various metal-chelating agents (pyrophosphate (PPi), <t>EDTA,</t> <t>EGTA)</t> and blocking effects of MgCl 2 . (c) Anti- S. aureus activities of ATP and various metal-chelating agents. (d) Ferric ion-chelating activities of ATP and EDTA measured by CAS assay. (e) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against M. intracellulare . (f) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against P. aeruginosa . (g) Siderophore production by ATP-resistant E. coli during the course of cultivation. (h) Siderophore production by two ATP-resistant K. pneumoniae strains during 48-h cultivation in the presence of ATP. (i) Siderophore production by ATP-susceptible S. aureus and ATP-resistant E. coli during 24-h cultivation in the presence of ATP (5 mM). (j) Siderophore production by ATP-susceptible and ATP-resistant strains of P. aeruginosa during 24-h cultivation.
    Egta, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 94/100, based on 81 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 81 article reviews
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    egta - by Bioz Stars, 2020-02
    94/100 stars
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    96
    Fisher Scientific egta
    Typical force versus relative deformation profiles for <t>MDA-MB-468</t> cells labeled with various dyes: a control cell (1, red), a <t>EGTA</t> treated cell (2, gray), and cells labeled with 5 μM CFDA-SE (3, blue), CMFDA (4, green), CMTMR (5, orange) and Calcein
    Egta, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 96/100, based on 216 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 216 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    92
    Fluka Chemical egta
    Typical force versus relative deformation profiles for <t>MDA-MB-468</t> cells labeled with various dyes: a control cell (1, red), a <t>EGTA</t> treated cell (2, gray), and cells labeled with 5 μM CFDA-SE (3, blue), CMFDA (4, green), CMTMR (5, orange) and Calcein
    Egta, supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 3 article reviews
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    egta - by Bioz Stars, 2020-02
    92/100 stars
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    99
    Millipore egta
    Activation of CaMKK β mediates the inhibition of calcium-dependent IL-2 production in RAW 264.7 cells. (a) Cells were treated with LPS in normal <t>DMEM,</t> calcium-free DMEM, calcium-free DMEM with 2 mM CaCl 2 , or DMEM with 5 mM <t>EGTA</t> for 30 min. Protein levels of CaMKK β and pCaMKK β were detected by western blot. Comparison was made for pCaMKK β /CaMKK β . ∗∗ P
    Egta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 17392 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 17392 article reviews
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    egta - by Bioz Stars, 2020-02
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    93
    Amresco egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    Egta, supplied by Amresco, used in various techniques. Bioz Stars score: 93/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 93 stars, based on 54 article reviews
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    egta - by Bioz Stars, 2020-02
    93/100 stars
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    96
    Euromedex egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    Egta, supplied by Euromedex, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 9 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    92
    RPI Inc egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    Egta, supplied by RPI Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/RPI Inc
    Average 92 stars, based on 1 article reviews
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    egta - by Bioz Stars, 2020-02
    92/100 stars
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    95
    Valiant egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    Egta, supplied by Valiant, used in various techniques. Bioz Stars score: 95/100, based on 52 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 95 stars, based on 52 article reviews
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    egta - by Bioz Stars, 2020-02
    95/100 stars
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    92
    Applichem egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    Egta, supplied by Applichem, used in various techniques. Bioz Stars score: 92/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2020-02
    92/100 stars
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    96
    Boehringer Mannheim egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    Egta, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 96/100, based on 778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 778 article reviews
    Price from $9.99 to $1999.99
    egta - by Bioz Stars, 2020-02
    96/100 stars
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    96
    FUJIFILM egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    Egta, supplied by FUJIFILM, used in various techniques. Bioz Stars score: 96/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    80
    Millipore • egta
    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of <t>BAPTA-AM</t> (20 μM), <t>EGTA</t> (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)
    • Egta, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 10 article reviews
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    • egta - by Bioz Stars, 2020-02
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    96
    Nacalai egta
    Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and <t>EGTA</t> or <t>EDTA</t> was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the
    Egta, supplied by Nacalai, used in various techniques. Bioz Stars score: 96/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 168 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    96
    Promega egta
    Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and <t>EGTA</t> or <t>EDTA</t> was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the
    Egta, supplied by Promega, used in various techniques. Bioz Stars score: 96/100, based on 276 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Promega
    Average 96 stars, based on 276 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    96
    Cell Signaling Technology Inc egta
    Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and <t>EGTA</t> or <t>EDTA</t> was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the
    Egta, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 830 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 830 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    96
    Merck & Co egta
    Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM <t>EGTA,</t> 5 mM Ni 2+ , 125 μM verapamil (VPL), or control <t>HBSS.</t> Results are means ± SEM for four to six experiments. ∗, P
    Egta, supplied by Merck & Co, used in various techniques. Bioz Stars score: 96/100, based on 112 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 96 stars, based on 112 article reviews
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    egta - by Bioz Stars, 2020-02
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    92
    Trion Research egta
    Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM <t>EGTA,</t> 5 mM Ni 2+ , 125 μM verapamil (VPL), or control <t>HBSS.</t> Results are means ± SEM for four to six experiments. ∗, P
    Egta, supplied by Trion Research, used in various techniques. Bioz Stars score: 92/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 92 stars, based on 20 article reviews
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    egta - by Bioz Stars, 2020-02
    92/100 stars
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    96
    Merck KGaA egta
    Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM <t>EGTA,</t> 5 mM Ni 2+ , 125 μM verapamil (VPL), or control <t>HBSS.</t> Results are means ± SEM for four to six experiments. ∗, P
    Egta, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 96/100, based on 129 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Merck KGaA
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    96
    AAT Bioquest egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by AAT Bioquest, used in various techniques. Bioz Stars score: 96/100, based on 48 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/AAT Bioquest
    Average 96 stars, based on 48 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    92
    Fluka Chemie egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by Fluka Chemie, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Fluka Chemie
    Average 92 stars, based on 7 article reviews
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    egta - by Bioz Stars, 2020-02
    92/100 stars
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    92
    ICN Biomedicals egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by ICN Biomedicals, used in various techniques. Bioz Stars score: 92/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/ICN Biomedicals
    Average 92 stars, based on 28 article reviews
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    egta - by Bioz Stars, 2020-02
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    egta  (Roche)
    99
    Roche egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 16065 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Roche
    Average 99 stars, based on 16065 article reviews
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    egta - by Bioz Stars, 2020-02
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    93
    BioShop egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by BioShop, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    egta - by Bioz Stars, 2020-02
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    92
    Fisher Bioreagents egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by Fisher Bioreagents, used in various techniques. Bioz Stars score: 92/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/Fisher Bioreagents
    Average 92 stars, based on 17 article reviews
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    egta - by Bioz Stars, 2020-02
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    egta  (TaKaRa)
    96
    TaKaRa egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/egta/product/TaKaRa
    Average 96 stars, based on 97 article reviews
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    egta - by Bioz Stars, 2020-02
    96/100 stars
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    92
    Avantor egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 54 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    egta  (Lonza)
    92
    Lonza egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by Lonza, used in various techniques. Bioz Stars score: 92/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Santa Cruz Biotechnology egta
    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with <t>EGTA/EGTA,AM,</t> and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .
    Egta, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 304 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Effect of PF-IgGs on binding of Dsg1-coated beads to HaCaT cells. Beads were allowed to settle on the surface of HaCaT cells for 30 minutes (control). Number of bound beads was reduced by simultaneous incubation of EGTA (5 mM, 30 minutes). Incubation of monolayers with attached beads for an additional 30 minutes with IgG fractions from 2 patients (PF1- and PF2-IgG, 35 μg/ml each) as well as with a monoclonal antibody (1:50) directed against the extracellular domain of human Dsg1 significantly reduced the number of bound beads (label in white box and white bars). Immunoabsorption using Dsg1-coated beads but not control absorption using VE-cadherin–labeled beads completely abolished the effect of PF-IgGs on bead adhesion. Preincubation of HaCaT cells with PF-IgGs prior to bead settlement also reduced bead binding (label in gray box and gray bars) whereas preincubation of beads with PF-IgGs did not inhibit bead binding. In contrast, the monoclonal Dsg1 antibody reduced bead binding also when applied for preincubation with beads, indicating a different mechanism underlying the reduction of Dsg1 adhesion ( n = 6 for each condition).

    Journal: Journal of Clinical Investigation

    Article Title: Pemphigus foliaceus IgG causes dissociation of desmoglein 1-containing junctions without blocking desmoglein 1 transinteraction

    doi: 10.1172/JCI23475

    Figure Lengend Snippet: Effect of PF-IgGs on binding of Dsg1-coated beads to HaCaT cells. Beads were allowed to settle on the surface of HaCaT cells for 30 minutes (control). Number of bound beads was reduced by simultaneous incubation of EGTA (5 mM, 30 minutes). Incubation of monolayers with attached beads for an additional 30 minutes with IgG fractions from 2 patients (PF1- and PF2-IgG, 35 μg/ml each) as well as with a monoclonal antibody (1:50) directed against the extracellular domain of human Dsg1 significantly reduced the number of bound beads (label in white box and white bars). Immunoabsorption using Dsg1-coated beads but not control absorption using VE-cadherin–labeled beads completely abolished the effect of PF-IgGs on bead adhesion. Preincubation of HaCaT cells with PF-IgGs prior to bead settlement also reduced bead binding (label in gray box and gray bars) whereas preincubation of beads with PF-IgGs did not inhibit bead binding. In contrast, the monoclonal Dsg1 antibody reduced bead binding also when applied for preincubation with beads, indicating a different mechanism underlying the reduction of Dsg1 adhesion ( n = 6 for each condition).

    Article Snippet: Negative controls were performed using Dsg1-coated beads incubated on the surface of HaCaT cells in the presence of 5 mM EGTA (Roth) or in the presence of monoclonal mouse IgG antibody (1:50 in HBSS) directed against Dsg-1 (clone p124; Progen Industries Ltd.).

    Techniques: Binding Assay, Incubation, Labeling

    ATP's antimicrobial effect is attributable to its iron-chelating activity. (a) Blocking effects of MgCl 2 and FeCl 3 on ATP's anti- M. intracellulare antimicrobial activity. (b) Anti- M. intracellulare activities of ATP and various metal-chelating agents (pyrophosphate (PPi), EDTA, EGTA) and blocking effects of MgCl 2 . (c) Anti- S. aureus activities of ATP and various metal-chelating agents. (d) Ferric ion-chelating activities of ATP and EDTA measured by CAS assay. (e) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against M. intracellulare . (f) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against P. aeruginosa . (g) Siderophore production by ATP-resistant E. coli during the course of cultivation. (h) Siderophore production by two ATP-resistant K. pneumoniae strains during 48-h cultivation in the presence of ATP. (i) Siderophore production by ATP-susceptible S. aureus and ATP-resistant E. coli during 24-h cultivation in the presence of ATP (5 mM). (j) Siderophore production by ATP-susceptible and ATP-resistant strains of P. aeruginosa during 24-h cultivation.

    Journal: Scientific Reports

    Article Title: ATP Exhibits Antimicrobial Action by Inhibiting Bacterial Utilization of Ferric Ions

    doi: 10.1038/srep08610

    Figure Lengend Snippet: ATP's antimicrobial effect is attributable to its iron-chelating activity. (a) Blocking effects of MgCl 2 and FeCl 3 on ATP's anti- M. intracellulare antimicrobial activity. (b) Anti- M. intracellulare activities of ATP and various metal-chelating agents (pyrophosphate (PPi), EDTA, EGTA) and blocking effects of MgCl 2 . (c) Anti- S. aureus activities of ATP and various metal-chelating agents. (d) Ferric ion-chelating activities of ATP and EDTA measured by CAS assay. (e) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against M. intracellulare . (f) Ferric ion-mediated reduction of antimicrobial effects of ATP and EDTA against P. aeruginosa . (g) Siderophore production by ATP-resistant E. coli during the course of cultivation. (h) Siderophore production by two ATP-resistant K. pneumoniae strains during 48-h cultivation in the presence of ATP. (i) Siderophore production by ATP-susceptible S. aureus and ATP-resistant E. coli during 24-h cultivation in the presence of ATP (5 mM). (j) Siderophore production by ATP-susceptible and ATP-resistant strains of P. aeruginosa during 24-h cultivation.

    Article Snippet: Special agents The following agents were used: ATP (Sigma Aldrich Co., St. Louis, MO; MP Biomedicals, Solon, OH, USA, Calbiochem Co., La Jolla, CA, and Roche Diagnostic Co., Indianapolis, IN), ADP (Sigma), AMP (Sigma), adenosine (Sigma), benzoylbenzoyl ATP (Sigma), oxidized ATP (Sigma), suramin (Wako, Tokyo, Japan), MIA (methyl isobutyl amiloride, Wako), DIDS (4,4′- Diisothiocyanatostilbene-2,2′-disulfonic acid, Sigma), EDTA (Dojindo, Tokyo, Japan), EGTA (Dojindo), dipyridyl (Sigma), CAS (Chrome Azurol S, Sigma), E. coli S17-1 and pK18mobSacB (kindly provided by Dr. F. Taguchi, Okayama University), Instagene matrix (Bio-Rad, Hercules, CA), KOD-Plus (Toyobo, Osaka, Japan), Wizard SV Gel and PCR cleanup system (Promeg, Madison, WI), Ligation High (Toyobo), Protein Assay Rapid Kit (Wako), [14 C] isoleucine (Moravek Biochemicals, Inc, Brea, CA), [14 C]uracil (Moravek Biochemicals, Inc.), α-defensin-1 (Peptide institute, Inc, Osaka, Japan.), cathepsin G (Sigma), vancomycin (Wako), clarithromycin (Taisho-Toyama Pharmaceutical Co., Tokyo), rifampin (Daiichi Sankyo Co., Tokyo), and ethambutol (Sigma), gatifloxacin (Wako), FLUOS (Sigma), LB medium (Invitrogen, San Diego, CA), M9 medium (prepared by our laboratory), Heart infusion agar (Eiken Chemical Co., Tokyo, Japan), Middlebrook 7H9 medium (Becton Dickinson, Cockeysville, MD), and Middlebrook 7H11 medium (Becton Dickinson).

    Techniques: Activity Assay, Blocking Assay

    Typical force versus relative deformation profiles for MDA-MB-468 cells labeled with various dyes: a control cell (1, red), a EGTA treated cell (2, gray), and cells labeled with 5 μM CFDA-SE (3, blue), CMFDA (4, green), CMTMR (5, orange) and Calcein

    Journal: The journal of physical chemistry. B

    Article Title: Cell tracing dyes significantly change single cell mechanics

    doi: 10.1021/jp8103358

    Figure Lengend Snippet: Typical force versus relative deformation profiles for MDA-MB-468 cells labeled with various dyes: a control cell (1, red), a EGTA treated cell (2, gray), and cells labeled with 5 μM CFDA-SE (3, blue), CMFDA (4, green), CMTMR (5, orange) and Calcein

    Article Snippet: To investigate role of calcium ions in cell stiffening MDA-MB-468 cells were incubated with 5 mM ethylene glycol tetraacetic acid, EGTA (Fisher Scientific, Fairlawn, NJ) for 30 min before compression.

    Techniques: Multiple Displacement Amplification, Labeling

    Activation of CaMKK β mediates the inhibition of calcium-dependent IL-2 production in RAW 264.7 cells. (a) Cells were treated with LPS in normal DMEM, calcium-free DMEM, calcium-free DMEM with 2 mM CaCl 2 , or DMEM with 5 mM EGTA for 30 min. Protein levels of CaMKK β and pCaMKK β were detected by western blot. Comparison was made for pCaMKK β /CaMKK β . ∗∗ P

    Journal: Mediators of Inflammation

    Article Title: Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKK β-AMPK-SIRT1 Signaling Pathway

    doi: 10.1155/2016/6152713

    Figure Lengend Snippet: Activation of CaMKK β mediates the inhibition of calcium-dependent IL-2 production in RAW 264.7 cells. (a) Cells were treated with LPS in normal DMEM, calcium-free DMEM, calcium-free DMEM with 2 mM CaCl 2 , or DMEM with 5 mM EGTA for 30 min. Protein levels of CaMKK β and pCaMKK β were detected by western blot. Comparison was made for pCaMKK β /CaMKK β . ∗∗ P

    Article Snippet: Cell Treatment For calcium modulation, macrophages were treated in calcium-free DMEM (GIBCO) or DMEM with EGTA (Sigma, USA) for calcium deprivation.

    Techniques: Activation Assay, Inhibition, Western Blot

    AMPK is activated downstream of CaMKK β for the negative control of the LPS induced IL-12 production in RAW 264.7 cells. (a) Cells were treated with LPS alone or with calcium-free DMEM, 2 mM CaCl 2 , 5 mM EGTA, or 1 μ M STO-609 for 30 min. Protein levels of AMPK α and pAMPK α were detected by western blot. ∗∗ P

    Journal: Mediators of Inflammation

    Article Title: Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKK β-AMPK-SIRT1 Signaling Pathway

    doi: 10.1155/2016/6152713

    Figure Lengend Snippet: AMPK is activated downstream of CaMKK β for the negative control of the LPS induced IL-12 production in RAW 264.7 cells. (a) Cells were treated with LPS alone or with calcium-free DMEM, 2 mM CaCl 2 , 5 mM EGTA, or 1 μ M STO-609 for 30 min. Protein levels of AMPK α and pAMPK α were detected by western blot. ∗∗ P

    Article Snippet: Cell Treatment For calcium modulation, macrophages were treated in calcium-free DMEM (GIBCO) or DMEM with EGTA (Sigma, USA) for calcium deprivation.

    Techniques: Negative Control, Western Blot

    LPS induced extracellular calcium influx and modulation of calcium influx affects IL-12 production in murine peritoneal macrophages. (a, b) Macrophages were incubated with LPS (1 μ g/mL) or LPS plus calcium-free DMEM, 5 mM EGTA, 5 μ M SKF96365, or 100 μ M ATP for 6 min. The fluorescence ratio (340 nm/380 nm) of Fura-2AM was captured by using the plate reader assay every 10 s ( n = 4). (c) Macrophages were treated with 5 μ M SKF96365 (SKF) or 100 μ M ATP for 1 h and then stimulated with 100 ng/mL LPS. Supernatants were collected 24 h later and IL-12 p40 and IL-12 p70 levels were detected by ELISA. ∗∗ P

    Journal: Mediators of Inflammation

    Article Title: Inhibition of Extracellular Calcium Influx Results in Enhanced IL-12 Production in LPS-Treated Murine Macrophages by Downregulation of the CaMKK β-AMPK-SIRT1 Signaling Pathway

    doi: 10.1155/2016/6152713

    Figure Lengend Snippet: LPS induced extracellular calcium influx and modulation of calcium influx affects IL-12 production in murine peritoneal macrophages. (a, b) Macrophages were incubated with LPS (1 μ g/mL) or LPS plus calcium-free DMEM, 5 mM EGTA, 5 μ M SKF96365, or 100 μ M ATP for 6 min. The fluorescence ratio (340 nm/380 nm) of Fura-2AM was captured by using the plate reader assay every 10 s ( n = 4). (c) Macrophages were treated with 5 μ M SKF96365 (SKF) or 100 μ M ATP for 1 h and then stimulated with 100 ng/mL LPS. Supernatants were collected 24 h later and IL-12 p40 and IL-12 p70 levels were detected by ELISA. ∗∗ P

    Article Snippet: Cell Treatment For calcium modulation, macrophages were treated in calcium-free DMEM (GIBCO) or DMEM with EGTA (Sigma, USA) for calcium deprivation.

    Techniques: Incubation, Fluorescence, Enzyme-linked Immunosorbent Assay

    Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)

    Journal: Molecules and Cells

    Article Title: Doxorubicin Induces the Persistent Activation of Intracellular Transglutaminase 2 That Protects from Cell Death

    doi: 10.1007/s10059-012-2201-9

    Figure Lengend Snippet: Doxorubicin-induced TG2 activation is inhibited by calcium chelators and caffeine. (A, B) The effect of BAPTA-AM (20 μM), EGTA (2 mM), or TLR2-neutralizing antibody (10 μg/ml) on TG activity after exposure to doxorubicin (1 μg/ml)

    Article Snippet: To identify the mediators of doxorubicin-induced TG2 activation, the cells were pre-treated for 1 h with 1 mM of N-acetylcysteine (NAC; Sigma), 20 μM BAPTA-AM (Molecular probes), 2 mM EGTA (Amresco), 30 μg/ml transforming growth factor β (TGFβ)-neutralizing antibody (R & D Systems), 10 μg/ml toll-like receptor 2 (TLR2)-neutralizing antibody (R & D Systems) or 0–20 mM caffeine (Sigma) before the doxorubicin treatment.

    Techniques: Activation Assay, Activity Assay

    Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and EGTA or EDTA was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the

    Journal: Infection and Immunity

    Article Title: Listeriolysin O-Dependent Bacterial Entry into the Cytoplasm Is Required for Calpain Activation and Interleukin-1? Secretion in Macrophages Infected with Listeria monocytogenes ▿

    doi: 10.1128/IAI.01143-09

    Figure Lengend Snippet: Involvement of intracellular calcium elevation in IL-1α secretion. Macrophages were infected with L. monocytogenes for 1 h, and EGTA or EDTA was added to the cultures at 3 h after infection (A). Alternatively, macrophages were infected with the

    Article Snippet: EDTA and EGTA were purchased from Nacalai Tesque Inc. (Kyoto, Japan).

    Techniques: Infection

    Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM EGTA, 5 mM Ni 2+ , 125 μM verapamil (VPL), or control HBSS. Results are means ± SEM for four to six experiments. ∗, P

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Interactions between HMR 3647, a New Ketolide, and Human Polymorphonuclear Neutrophils

    doi:

    Figure Lengend Snippet: Effect of Ca 2+ chelation and Ca 2+ channel inhibitors on HMR 3647 uptake. (A) PMNs were incubated for 5 to 60 min in the presence of 1 mM EGTA, 5 mM Ni 2+ , 125 μM verapamil (VPL), or control HBSS. Results are means ± SEM for four to six experiments. ∗, P

    Article Snippet: The uptake kinetics of macrolides was assessed first in Ca2+ -depleted HBSS (Gibco), supplemented with 1 mM EGTA (Merck), 1 mM magnesium chloride (Merck), and 4.2 mM sodium bicarbonate (NaHCO3 ) (Diagnostic Pasteur).

    Techniques: Incubation

    Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with EGTA/EGTA,AM, and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .

    Journal: Cell reports

    Article Title: Distortion of the Actin A-Triad Results in Contractile Disinhibition and Cardiomyopathy

    doi: 10.1016/j.celrep.2017.08.070

    Figure Lengend Snippet: Excessive Ca 2+ -Independent Actomyosin Associations during Diastole Promote Enhanced Myocyte Shortening and Incomplete Relaxation of Hand > Act57B A295S Cardiomyocytes (A) M modes generated from the same region of a three-week-old Hand > Act57B WT heart reveal graded responses of cardiac diameters following exposure to distinct small-molecule compounds. Red arrowheads indicate the position of the heart wall edges during diastole, upon incubation with EGTA/EGTA,AM, and finally, following the addition of blebbistatin. Incubation with EGTA/EGTA,AM resulted in complete cessation of wall motion. Relative to the diameter during diastole, each treatment induced a slight increase in diameter across the heart tube. (B) Significant, incremental increases in cardiac diameters were verified for all genotypes following extra- and intracellular Ca 2+ chelation and upon blebbistatin incubation. (C) The average change in diameter across the heart wall in response to EGTA/EGTA,AM was similar among all lines. (D) Blebbistatin treatment prompted a significantly greater response across the wall of Hand > Act57B A295S hearts relative to that observed for Hand × yw and Hand > Act57B WT hearts. Data are presented as mean ± SEM (n = 21). ***p ≤ 0.001. See also Figure S3 .

    Article Snippet: After initial filming, the AH was replaced with AH containing 10 mM EGTA and 100 μM EGTA,AM (AAT Bioquest).

    Techniques: Generated, Incubation