Article Title: Epigallocatechin-3-gallate and 6-OH-11-O-Hydroxyphenanthrene Limit BE(2)-C Neuroblastoma Cell Growth and Neurosphere Formation In Vitro
Figure Lengend Snippet: Downregulation of EGFR and p1068EGFR expression by EGCG and IIF treatments in BE(2)-C neuroblastoma cells. Cells were treated with 20 μg/mL EGCG and 10 μM IIF, individually and in combination for 24 h. ( A ) Proteins (50 μg) from total cell lysate were subjected to SDS–PAGE and Western blot analysis of EGFR and p1068EGFR expression after 24 h treatments. Actin was used as a loading control. RT-PCR analysis of EGFR ( B ) and NDRG1 ( C ) in control and treated cells. β-actin was used as a control. The values were normalized to the untreated controls. The results are expressed as the average ± SE of three independent experiments. * p
Article Snippet: Antibodies: anti-RARα and anti-RXRγ (Tema Ricerca, Bologna, Italy), anti-EGFR (Thermo Scientific, Waltham, MA, USA), anti-p1068EGFR (Novex, Life Technologies, Carlsbad, CA, USA), anti-Bcl-2 (Sigma-Aldrich, St. Louis, MO, USA), anti-Bax (Applied Biosystem, Monza, Italy) anti-PARP (Santa Cruz Biotechnology, Dallas, TX, USA), anti-COX-2 (Sigma-Aldrich, St. Louis, MO, USA), anti-N-MYC, anti MMP-2, MMP-9, and anti-TIMP-1 (all from Santa Cruz Biotechnology, Dallas, TX, USA), anti-β-tubulin (Sigma-Aldrich, St. Louis, MO, USA), anti-rabbit, and anti-mouse peroxidase conjugated antibodies (GE Healthcare, Milan, Italy).
Techniques: Expressing, SDS Page, Western Blot, Reverse Transcription Polymerase Chain Reaction