egf dependent transcriptome Search Results


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  • 99
    Qiagen sensiscript reverse transcriptase kit
    Sensiscript Reverse Transcriptase Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 174 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs m mulv reverse transcriptase
    M Mulv Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3547 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore epidermal growth factor receptor egfr
    A hypothetical model for the proposed signaling network involved in E 2 -BSA-induced ESC migration. E 2 -BSA increases <t>FAK</t> activity as well as <t>EGFR</t> phosphorylation via c-Src. Membrane ER-dependent FAK and EGFR-dependent N-WASP/cdc42/TOCA-1 signaling pathways stimulated profilin-1 expression and cofilin-1 phosphorylation, which increased F-actin expression levels. E 2 -BSA is estradiol-6- O -carboxymethyloxime-BSA.
    Epidermal Growth Factor Receptor Egfr, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore anti egf
    Modulation of the expression of SOCS-3, IRF-1, EGFR, and <t>STAT-3</t> proteins in HGEC infected with P. gingivalis and activated by LPS- Pg . (a) HGEC were infected for 2 h with P. gingivalis at a MOI of 100 and with heat-killed P. gingivalis (Ina). Uninfected cells served as control (C). (b) HGEC were activated 2 and 24 h by 1 µg/mL of purified LPS- Pg . Non-activated cells served as controls (C). In both experiments, cellular extracts were prepared and analyzed by immunoblotting with antibodies to SOCS-3, IRF-1, EGFR, and STAT-3. No detection was obtained using an antibody raised against <t>EGF</t> (not shown). An antibody to GAPDH was used as an internal control to verify equal loading of total proteins in all wells. Histograms indicated the relative protein expression level during infection and activation. Levels were determined by pixel intensity of a protein band normalized to the intensity of the internal control GAPDH within the same assay. Differences (*) between a given ratio and the one obtained with control cells were analyzed with Student’s t -test ( p
    Anti Egf, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Novoprotein egf
    Modulation of the expression of SOCS-3, IRF-1, EGFR, and <t>STAT-3</t> proteins in HGEC infected with P. gingivalis and activated by LPS- Pg . (a) HGEC were infected for 2 h with P. gingivalis at a MOI of 100 and with heat-killed P. gingivalis (Ina). Uninfected cells served as control (C). (b) HGEC were activated 2 and 24 h by 1 µg/mL of purified LPS- Pg . Non-activated cells served as controls (C). In both experiments, cellular extracts were prepared and analyzed by immunoblotting with antibodies to SOCS-3, IRF-1, EGFR, and STAT-3. No detection was obtained using an antibody raised against <t>EGF</t> (not shown). An antibody to GAPDH was used as an internal control to verify equal loading of total proteins in all wells. Histograms indicated the relative protein expression level during infection and activation. Levels were determined by pixel intensity of a protein band normalized to the intensity of the internal control GAPDH within the same assay. Differences (*) between a given ratio and the one obtained with control cells were analyzed with Student’s t -test ( p
    Egf, supplied by Novoprotein, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Abcam epidermal growth factor receptor egfr
    Modulation of the expression of SOCS-3, IRF-1, EGFR, and <t>STAT-3</t> proteins in HGEC infected with P. gingivalis and activated by LPS- Pg . (a) HGEC were infected for 2 h with P. gingivalis at a MOI of 100 and with heat-killed P. gingivalis (Ina). Uninfected cells served as control (C). (b) HGEC were activated 2 and 24 h by 1 µg/mL of purified LPS- Pg . Non-activated cells served as controls (C). In both experiments, cellular extracts were prepared and analyzed by immunoblotting with antibodies to SOCS-3, IRF-1, EGFR, and STAT-3. No detection was obtained using an antibody raised against <t>EGF</t> (not shown). An antibody to GAPDH was used as an internal control to verify equal loading of total proteins in all wells. Histograms indicated the relative protein expression level during infection and activation. Levels were determined by pixel intensity of a protein band normalized to the intensity of the internal control GAPDH within the same assay. Differences (*) between a given ratio and the one obtained with control cells were analyzed with Student’s t -test ( p
    Epidermal Growth Factor Receptor Egfr, supplied by Abcam, used in various techniques. Bioz Stars score: 94/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    BioCarta biocarta egf pathway
    Modulation of the expression of SOCS-3, IRF-1, EGFR, and <t>STAT-3</t> proteins in HGEC infected with P. gingivalis and activated by LPS- Pg . (a) HGEC were infected for 2 h with P. gingivalis at a MOI of 100 and with heat-killed P. gingivalis (Ina). Uninfected cells served as control (C). (b) HGEC were activated 2 and 24 h by 1 µg/mL of purified LPS- Pg . Non-activated cells served as controls (C). In both experiments, cellular extracts were prepared and analyzed by immunoblotting with antibodies to SOCS-3, IRF-1, EGFR, and STAT-3. No detection was obtained using an antibody raised against <t>EGF</t> (not shown). An antibody to GAPDH was used as an internal control to verify equal loading of total proteins in all wells. Histograms indicated the relative protein expression level during infection and activation. Levels were determined by pixel intensity of a protein band normalized to the intensity of the internal control GAPDH within the same assay. Differences (*) between a given ratio and the one obtained with control cells were analyzed with Student’s t -test ( p
    Biocarta Egf Pathway, supplied by BioCarta, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher human recombinant epidermal growth factor 1 53
    Modulation of the expression of SOCS-3, IRF-1, EGFR, and <t>STAT-3</t> proteins in HGEC infected with P. gingivalis and activated by LPS- Pg . (a) HGEC were infected for 2 h with P. gingivalis at a MOI of 100 and with heat-killed P. gingivalis (Ina). Uninfected cells served as control (C). (b) HGEC were activated 2 and 24 h by 1 µg/mL of purified LPS- Pg . Non-activated cells served as controls (C). In both experiments, cellular extracts were prepared and analyzed by immunoblotting with antibodies to SOCS-3, IRF-1, EGFR, and STAT-3. No detection was obtained using an antibody raised against <t>EGF</t> (not shown). An antibody to GAPDH was used as an internal control to verify equal loading of total proteins in all wells. Histograms indicated the relative protein expression level during infection and activation. Levels were determined by pixel intensity of a protein band normalized to the intensity of the internal control GAPDH within the same assay. Differences (*) between a given ratio and the one obtained with control cells were analyzed with Student’s t -test ( p
    Human Recombinant Epidermal Growth Factor 1 53, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Illumina Inc gene expression profiling dge
    <t>Microarray</t> versus <t>DGE</t> analysis . (A) Overlap of unique and named genes shared among the 3 microarray platforms and genes detected by DGE. The pool of 14645 shared genes was used for further cross-platform analysis. The total numbers of genes for each platform and for all platforms combined are indicated. (B) Overlap of significantly regulated genes considering the 3 microarray platforms at 6 h after EGF treatment and the genes found regulated after assessing significance by grouping microarray and DGE data in a RankProd analysis. Left panels show up-regulated genes and right panels show down-regulated genes.
    Gene Expression Profiling Dge, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 94/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Novoprotein egf signal transduction
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Egf Signal Transduction, supplied by Novoprotein, used in various techniques. Bioz Stars score: 86/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega goscript reverse transcriptase system
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    Promega amv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Amv Reverse Transcriptase, supplied by Promega, used in various techniques. Bioz Stars score: 99/100, based on 6657 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega m mlv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    Solexa solexa based transcriptomics
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Solexa Based Transcriptomics, supplied by Solexa, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs protoscript ii reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Protoscript Ii Reverse Transcriptase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 1197 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscriptii reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Superscriptii Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2397 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher m mlv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    Thermo Fisher mulv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Mulv Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2615 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa amv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    Stratagene m mulv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    Thermo Fisher mumlv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    PerkinElmer multiscribetm reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Multiscribetm Reverse Transcriptase, supplied by PerkinElmer, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare avian myeloblastosis virus reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Avian Myeloblastosis Virus Reverse Transcriptase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 228 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript rnase h reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Superscript Rnase H Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 833 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher moloney murine leukemia virus reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
    Moloney Murine Leukemia Virus Reverse Transcriptase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12022 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega moloney murine leukemia virus reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    Cell Signaling Technology Inc snail family transcriptional repressor 2 snai2
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    GE Healthcare m mulv reverse transcriptase
    <t>E6</t> splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for <t>EGF,</t> and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.
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    Image Search Results


    A hypothetical model for the proposed signaling network involved in E 2 -BSA-induced ESC migration. E 2 -BSA increases FAK activity as well as EGFR phosphorylation via c-Src. Membrane ER-dependent FAK and EGFR-dependent N-WASP/cdc42/TOCA-1 signaling pathways stimulated profilin-1 expression and cofilin-1 phosphorylation, which increased F-actin expression levels. E 2 -BSA is estradiol-6- O -carboxymethyloxime-BSA.

    Journal: Molecular Endocrinology

    Article Title: Rapid Actions of Plasma Membrane Estrogen Receptors Regulate Motility of Mouse Embryonic Stem Cells through a Profilin-1/Cofilin-1-Directed Kinase Signaling Pathway

    doi: 10.1210/me.2012-1002

    Figure Lengend Snippet: A hypothetical model for the proposed signaling network involved in E 2 -BSA-induced ESC migration. E 2 -BSA increases FAK activity as well as EGFR phosphorylation via c-Src. Membrane ER-dependent FAK and EGFR-dependent N-WASP/cdc42/TOCA-1 signaling pathways stimulated profilin-1 expression and cofilin-1 phosphorylation, which increased F-actin expression levels. E 2 -BSA is estradiol-6- O -carboxymethyloxime-BSA.

    Article Snippet: Anti-Nanog, octamer-binding transcription factor 4 (Oct4), stage-specific embryonic antigen-1 (SSEA-1), focal adhesion kinase (FAK), ER-α, ER-β, phospho-FAK, c-Src, phospho-c-Src, epidermal growth factor receptor (EGFR), phospho-EGFR, cdc42, neural Wiskott-Aldrich syndrome protein (N-WASP), transducer of cdc42-dependent actin assembly-1 (TOCA-1), profilin-1, cofilin, phospho-cofilin-1, lamin A/C, pan-cadherin, and β-actin were purchased from Santa Cruz Biotechnology (Santa Cruz, CA).

    Techniques: Migration, Activity Assay, Expressing

    Modulation of the expression of SOCS-3, IRF-1, EGFR, and STAT-3 proteins in HGEC infected with P. gingivalis and activated by LPS- Pg . (a) HGEC were infected for 2 h with P. gingivalis at a MOI of 100 and with heat-killed P. gingivalis (Ina). Uninfected cells served as control (C). (b) HGEC were activated 2 and 24 h by 1 µg/mL of purified LPS- Pg . Non-activated cells served as controls (C). In both experiments, cellular extracts were prepared and analyzed by immunoblotting with antibodies to SOCS-3, IRF-1, EGFR, and STAT-3. No detection was obtained using an antibody raised against EGF (not shown). An antibody to GAPDH was used as an internal control to verify equal loading of total proteins in all wells. Histograms indicated the relative protein expression level during infection and activation. Levels were determined by pixel intensity of a protein band normalized to the intensity of the internal control GAPDH within the same assay. Differences (*) between a given ratio and the one obtained with control cells were analyzed with Student’s t -test ( p

    Journal: Journal of Oral Microbiology

    Article Title: Porphyromonas gingivalis and its lipopolysaccharide differently modulate epidermal growth factor–dependent signaling in human gingival epithelial cells

    doi: 10.1080/20002297.2017.1334503

    Figure Lengend Snippet: Modulation of the expression of SOCS-3, IRF-1, EGFR, and STAT-3 proteins in HGEC infected with P. gingivalis and activated by LPS- Pg . (a) HGEC were infected for 2 h with P. gingivalis at a MOI of 100 and with heat-killed P. gingivalis (Ina). Uninfected cells served as control (C). (b) HGEC were activated 2 and 24 h by 1 µg/mL of purified LPS- Pg . Non-activated cells served as controls (C). In both experiments, cellular extracts were prepared and analyzed by immunoblotting with antibodies to SOCS-3, IRF-1, EGFR, and STAT-3. No detection was obtained using an antibody raised against EGF (not shown). An antibody to GAPDH was used as an internal control to verify equal loading of total proteins in all wells. Histograms indicated the relative protein expression level during infection and activation. Levels were determined by pixel intensity of a protein band normalized to the intensity of the internal control GAPDH within the same assay. Differences (*) between a given ratio and the one obtained with control cells were analyzed with Student’s t -test ( p

    Article Snippet: Except for the anti-EGF (cat: WHOOO1950M1), the anti-STAT-3 (cat: 07–2173), and the anti-phospho-STAT-3 (pTyr705 ; cat: SAB4300033), which were obtained from Sigma–Aldrich, all other primary and secondary antibodies were purchased from Proteintech Europe (Manchester, United Kingdom) and were used at the concentrations recommended by the furnisher: anti-IRF-1 polyclonal antibody (cat: 11335–1-AP); anti-SOCS-3 polyclonal antibody (cat: 14025–1-AP); anti-EGFR (cat: 18986–1-AP); and anti-GAPDH polyclonal antibody (cat: 10494–1-AP).

    Techniques: Expressing, Infection, Purification, Activation Assay

    Modulation of the expression of microRNAs (mRNAs) encoding cytokine signaling-3 (SOCS-3), interferon regulatory factor-1 (IRF-1), epidermal growth factor (EGF), EGF-receptor (EGFR), and signal transducers and activators of transcription (STAT-3) in human gingival epithelial cells (HGEC) infected with Porphyromonas gingivalis and activated by its lipopolysaccharide (LPS- Pg ). (a) HGEC were infected for 2 h with P. gingivalis at different multiplicities of infection (MOI; 10 or 100) and with heat-killed P. gingivalis (Ina) at a MOI of 100. Uninfected cells served as control. (b) HGEC were activated for 2 h and 24 h by 0.5 µg/mL and 1 µg/mL of LPS- Pg . Unactivated cells served as controls. In both experiments, total RNA were prepared, and levels of mRNAs encoding SOCS-3, IRF-1, EGF, EGFR, and STAT-3 were determined by quantitative reverse transcription polymerase chain reaction analysis. All results were presented as the quantity relative to β-actin as a reference gene. Differences (*) between a test mRNA and the control HGEC were analyzed with Student’s t -test ( p

    Journal: Journal of Oral Microbiology

    Article Title: Porphyromonas gingivalis and its lipopolysaccharide differently modulate epidermal growth factor–dependent signaling in human gingival epithelial cells

    doi: 10.1080/20002297.2017.1334503

    Figure Lengend Snippet: Modulation of the expression of microRNAs (mRNAs) encoding cytokine signaling-3 (SOCS-3), interferon regulatory factor-1 (IRF-1), epidermal growth factor (EGF), EGF-receptor (EGFR), and signal transducers and activators of transcription (STAT-3) in human gingival epithelial cells (HGEC) infected with Porphyromonas gingivalis and activated by its lipopolysaccharide (LPS- Pg ). (a) HGEC were infected for 2 h with P. gingivalis at different multiplicities of infection (MOI; 10 or 100) and with heat-killed P. gingivalis (Ina) at a MOI of 100. Uninfected cells served as control. (b) HGEC were activated for 2 h and 24 h by 0.5 µg/mL and 1 µg/mL of LPS- Pg . Unactivated cells served as controls. In both experiments, total RNA were prepared, and levels of mRNAs encoding SOCS-3, IRF-1, EGF, EGFR, and STAT-3 were determined by quantitative reverse transcription polymerase chain reaction analysis. All results were presented as the quantity relative to β-actin as a reference gene. Differences (*) between a test mRNA and the control HGEC were analyzed with Student’s t -test ( p

    Article Snippet: Except for the anti-EGF (cat: WHOOO1950M1), the anti-STAT-3 (cat: 07–2173), and the anti-phospho-STAT-3 (pTyr705 ; cat: SAB4300033), which were obtained from Sigma–Aldrich, all other primary and secondary antibodies were purchased from Proteintech Europe (Manchester, United Kingdom) and were used at the concentrations recommended by the furnisher: anti-IRF-1 polyclonal antibody (cat: 11335–1-AP); anti-SOCS-3 polyclonal antibody (cat: 14025–1-AP); anti-EGFR (cat: 18986–1-AP); and anti-GAPDH polyclonal antibody (cat: 10494–1-AP).

    Techniques: Expressing, Infection, Reverse Transcription Polymerase Chain Reaction

    Microarray versus DGE analysis . (A) Overlap of unique and named genes shared among the 3 microarray platforms and genes detected by DGE. The pool of 14645 shared genes was used for further cross-platform analysis. The total numbers of genes for each platform and for all platforms combined are indicated. (B) Overlap of significantly regulated genes considering the 3 microarray platforms at 6 h after EGF treatment and the genes found regulated after assessing significance by grouping microarray and DGE data in a RankProd analysis. Left panels show up-regulated genes and right panels show down-regulated genes.

    Journal: BMC Genomics

    Article Title: Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis

    doi: 10.1186/1471-2164-12-326

    Figure Lengend Snippet: Microarray versus DGE analysis . (A) Overlap of unique and named genes shared among the 3 microarray platforms and genes detected by DGE. The pool of 14645 shared genes was used for further cross-platform analysis. The total numbers of genes for each platform and for all platforms combined are indicated. (B) Overlap of significantly regulated genes considering the 3 microarray platforms at 6 h after EGF treatment and the genes found regulated after assessing significance by grouping microarray and DGE data in a RankProd analysis. Left panels show up-regulated genes and right panels show down-regulated genes.

    Article Snippet: Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

    Techniques: Microarray

    Correlation between microarrays and Illumina GA-I sequencing . (A) Comparison of estimated log2ratios from DGE ( Y -axis) and the mean of all microarray platforms ( X -axis). We consider only genes that were interrogated using all platforms and genes with a mean number of counts across lanes greater than 0. Genes with counts greater than 32 reads (colored red or green) or less than (black) 32 reads in at least one sample are shown. (Red dots) Genes called differentially expressed based on DGE data at an 10% FDR by RankProd. (Green dots) Genes not called as differentially expressed but above 32 counts. (Inset box) Correlation between technologies is higher when considering genes above the 32 count detection level (0.57) than when all genes are included (0.49). (B-C) Concordance at the top (CAT) plots of the different platforms with the 500 top genes from a reference platform, shown for Agilent in (B) and DGE in (C). See inset box for color codes identifying each platforms compared to the remaining platform used as reference. (D) Correlation plots with regression lines between log2ratios of the five high content platforms measurements (Y-axis) and quantitative real time PCR results using SYBR green assays (X-axis), based on measurements for 21 genes at the 6 h time point (see Additional file 2 , Table S1).

    Journal: BMC Genomics

    Article Title: Multiple platform assessment of the EGF dependent transcriptome by microarray and deep tag sequencing analysis

    doi: 10.1186/1471-2164-12-326

    Figure Lengend Snippet: Correlation between microarrays and Illumina GA-I sequencing . (A) Comparison of estimated log2ratios from DGE ( Y -axis) and the mean of all microarray platforms ( X -axis). We consider only genes that were interrogated using all platforms and genes with a mean number of counts across lanes greater than 0. Genes with counts greater than 32 reads (colored red or green) or less than (black) 32 reads in at least one sample are shown. (Red dots) Genes called differentially expressed based on DGE data at an 10% FDR by RankProd. (Green dots) Genes not called as differentially expressed but above 32 counts. (Inset box) Correlation between technologies is higher when considering genes above the 32 count detection level (0.57) than when all genes are included (0.49). (B-C) Concordance at the top (CAT) plots of the different platforms with the 500 top genes from a reference platform, shown for Agilent in (B) and DGE in (C). See inset box for color codes identifying each platforms compared to the remaining platform used as reference. (D) Correlation plots with regression lines between log2ratios of the five high content platforms measurements (Y-axis) and quantitative real time PCR results using SYBR green assays (X-axis), based on measurements for 21 genes at the 6 h time point (see Additional file 2 , Table S1).

    Article Snippet: Here we use a combined approach to study the EGF dependent transcriptome of HeLa cells by using multiple long oligonucleotide based microarray platforms (from Agilent, Operon, and Illumina) in combination with digital gene expression profiling (DGE) with the Illumina Genome Analyzer.

    Techniques: Sequencing, Microarray, Real-time Polymerase Chain Reaction, SYBR Green Assay

    E6 splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for EGF, and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation

    doi: 10.1073/pnas.1002620107

    Figure Lengend Snippet: E6 splicing pattern is not dependent on mRNA transport, RNA stability, or promoter regulation. ( A ) “1321” cells were depleted for EGF, and nuclear RNA (N) or total RNA (T) was harvested for RT-PCR analysis. ( B ) “1321” cells were treated with actinomycin D (5 μg/mL) for indicated periods of time. Cytoplasmic RNA was harvested and E6*/E6 levels were determined with RT-PCR. c-Jun was analyzed as a control. ( C ) “1637” cells were depleted from EGF or treated with MEK1 inhibitor U0126 for 24 h. Cytoplasmic RNA was extracted for RT-PCR. ( D ) RT-PCR showing E6, E6*, and GAPDH cytoplasmic mRNA levels in FK16A cells in presence and absence of EGF.

    Article Snippet: Time-course experiments and incubation with cycloheximide demonstrated that E6 alternative splicing is a direct and reversible effect of EGF signal transduction, not depending on de novo protein synthesis.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    EGF depletion leads to HPV16 E6 exon exclusion. “1321” cells were cultured in presence or absence of EGF for 24 h. ( A ) RT-PCR showing E6, E6*, p53, and GAPDH cytoplasmic mRNA levels. ( B ) Western blot analysis of total protein levels of p53, E7, pRb, and actin.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation

    doi: 10.1073/pnas.1002620107

    Figure Lengend Snippet: EGF depletion leads to HPV16 E6 exon exclusion. “1321” cells were cultured in presence or absence of EGF for 24 h. ( A ) RT-PCR showing E6, E6*, p53, and GAPDH cytoplasmic mRNA levels. ( B ) Western blot analysis of total protein levels of p53, E7, pRb, and actin.

    Article Snippet: Time-course experiments and incubation with cycloheximide demonstrated that E6 alternative splicing is a direct and reversible effect of EGF signal transduction, not depending on de novo protein synthesis.

    Techniques: Cell Culture, Reverse Transcription Polymerase Chain Reaction, Western Blot

    HPV16 E6 alternative splicing is directly mediated by EGFR signaling. ( A ) “1321” cells were treated with neutralizing anti-EGF receptor antibody or the specific EGF receptor inhibitor AG1478 for 24 h. ( B ) “1321” cells were grown in presence of indicated amounts of EGF for 24 h. ( C and D ) Time course of E6/E6* splicing after EGF depletion ( C ) or readdition ( D ). ( E ) “1321” cells were pretreated with cycloheximide (CHX) for 1.5 h. Subsequently, EGF was depleted for additional 5 h in the presence of CHX. In each case, cytoplasmic RNA was analyzed by RT-PCR.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation

    doi: 10.1073/pnas.1002620107

    Figure Lengend Snippet: HPV16 E6 alternative splicing is directly mediated by EGFR signaling. ( A ) “1321” cells were treated with neutralizing anti-EGF receptor antibody or the specific EGF receptor inhibitor AG1478 for 24 h. ( B ) “1321” cells were grown in presence of indicated amounts of EGF for 24 h. ( C and D ) Time course of E6/E6* splicing after EGF depletion ( C ) or readdition ( D ). ( E ) “1321” cells were pretreated with cycloheximide (CHX) for 1.5 h. Subsequently, EGF was depleted for additional 5 h in the presence of CHX. In each case, cytoplasmic RNA was analyzed by RT-PCR.

    Article Snippet: Time-course experiments and incubation with cycloheximide demonstrated that E6 alternative splicing is a direct and reversible effect of EGF signal transduction, not depending on de novo protein synthesis.

    Techniques: Reverse Transcription Polymerase Chain Reaction

    siRNA knockdowns of splicing factors. “1321” keratinocytes were transfected with the indicated siRNAs as described. Cytoplasmic RNA was harvested and analyzed by RT-PCR. ( A ) Splicing factors found to promote exon inclusion were analyzed in the presence of EGF. (B) hnRNP knockdown in the presence and absence of EGF. Band intensities were quantified from three independent experiments using ImageJ and E6*/E6 ratios were calculated. Ordinate: percentage of the E6*/E6 ratio, where values for untransfected samples were set as 100%. Abscissa: (−), untransfected; (c), scramble siRNA transfection.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation

    doi: 10.1073/pnas.1002620107

    Figure Lengend Snippet: siRNA knockdowns of splicing factors. “1321” keratinocytes were transfected with the indicated siRNAs as described. Cytoplasmic RNA was harvested and analyzed by RT-PCR. ( A ) Splicing factors found to promote exon inclusion were analyzed in the presence of EGF. (B) hnRNP knockdown in the presence and absence of EGF. Band intensities were quantified from three independent experiments using ImageJ and E6*/E6 ratios were calculated. Ordinate: percentage of the E6*/E6 ratio, where values for untransfected samples were set as 100%. Abscissa: (−), untransfected; (c), scramble siRNA transfection.

    Article Snippet: Time-course experiments and incubation with cycloheximide demonstrated that E6 alternative splicing is a direct and reversible effect of EGF signal transduction, not depending on de novo protein synthesis.

    Techniques: Transfection, Reverse Transcription Polymerase Chain Reaction

    EGF-regulated E6 alternative splicing is mediated by MAP kinase signaling. ( A ) “1321” keratinocytes were depleted from EGF or treated with various inhibitors of the EGFR signaling pathways as indicated for 24 h. Cytoplasmic RNA was harvested and E6 splicing pattern analyzed by RT-PCR. ( B ) Phospho-MAPK Array (R D Systems) showing the phosphorylation status of Erk1/2 and Akt1/2/3, respectively, after 24 h in presence of absence of EGF. Whole-cell extracts were prepared and processed as described. ( C ) 293 cells were cotransfected with 2 μg pLXSN-E6 and either of 5 μg pEGFP or with a MEK1 dominant negative mutant, respectively. Twenty-four hours posttransfection, cells were harvested and cytoplasmic RNA was extracted for RT-PCR analysis.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Alternative splicing of human papillomavirus type-16 E6/E6* early mRNA is coupled to EGF signaling via Erk1/2 activation

    doi: 10.1073/pnas.1002620107

    Figure Lengend Snippet: EGF-regulated E6 alternative splicing is mediated by MAP kinase signaling. ( A ) “1321” keratinocytes were depleted from EGF or treated with various inhibitors of the EGFR signaling pathways as indicated for 24 h. Cytoplasmic RNA was harvested and E6 splicing pattern analyzed by RT-PCR. ( B ) Phospho-MAPK Array (R D Systems) showing the phosphorylation status of Erk1/2 and Akt1/2/3, respectively, after 24 h in presence of absence of EGF. Whole-cell extracts were prepared and processed as described. ( C ) 293 cells were cotransfected with 2 μg pLXSN-E6 and either of 5 μg pEGFP or with a MEK1 dominant negative mutant, respectively. Twenty-four hours posttransfection, cells were harvested and cytoplasmic RNA was extracted for RT-PCR analysis.

    Article Snippet: Time-course experiments and incubation with cycloheximide demonstrated that E6 alternative splicing is a direct and reversible effect of EGF signal transduction, not depending on de novo protein synthesis.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Dominant Negative Mutation