ef1a qrt-pcrs Search Results


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  • 99
    Thermo Fisher maxima sybr green rox qpcr master mix 2×
    Maxima Sybr Green Rox Qpcr Master Mix 2×, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 612 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co qrt pcr
    Qrt Pcr, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche qrt pcr
    TaFT3 homoeolog-specific <t>qRT-PCR</t> gene expression in hexaploid wheat cultivars Cadenza (A–C) and Chinese Spring (D–F) and tetraploid wheat cultivar Kronos (G,H) grown under LD (white circles) and SD (black circles) photoperiods . Leaf samples for expression analyses were harvested at 0, 1, 2, 3 and 4 weeks. qRT-PCR TaFT3 expression data is normalized against four control genes ( ACTIN, UBIQUITIN, GAPDH, EF1A ). ± 1 standard error of the mean (SEM) indicated.
    Qrt Pcr, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 4253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    MJ Research qrt pcr
    TaFT3 homoeolog-specific <t>qRT-PCR</t> gene expression in hexaploid wheat cultivars Cadenza (A–C) and Chinese Spring (D–F) and tetraploid wheat cultivar Kronos (G,H) grown under LD (white circles) and SD (black circles) photoperiods . Leaf samples for expression analyses were harvested at 0, 1, 2, 3 and 4 weeks. qRT-PCR TaFT3 expression data is normalized against four control genes ( ACTIN, UBIQUITIN, GAPDH, EF1A ). ± 1 standard error of the mean (SEM) indicated.
    Qrt Pcr, supplied by MJ Research, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa qrt pcr
    TaFT3 homoeolog-specific <t>qRT-PCR</t> gene expression in hexaploid wheat cultivars Cadenza (A–C) and Chinese Spring (D–F) and tetraploid wheat cultivar Kronos (G,H) grown under LD (white circles) and SD (black circles) photoperiods . Leaf samples for expression analyses were harvested at 0, 1, 2, 3 and 4 weeks. qRT-PCR TaFT3 expression data is normalized against four control genes ( ACTIN, UBIQUITIN, GAPDH, EF1A ). ± 1 standard error of the mean (SEM) indicated.
    Qrt Pcr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 97/100, based on 4523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene qrt pcr
    TaFT3 homoeolog-specific <t>qRT-PCR</t> gene expression in hexaploid wheat cultivars Cadenza (A–C) and Chinese Spring (D–F) and tetraploid wheat cultivar Kronos (G,H) grown under LD (white circles) and SD (black circles) photoperiods . Leaf samples for expression analyses were harvested at 0, 1, 2, 3 and 4 weeks. qRT-PCR TaFT3 expression data is normalized against four control genes ( ACTIN, UBIQUITIN, GAPDH, EF1A ). ± 1 standard error of the mean (SEM) indicated.
    Qrt Pcr, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad qrt pcr
    Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for <t>qRT-PCR</t> analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01
    Qrt Pcr, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 97/100, based on 7399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech qrt pcr
    Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for <t>qRT-PCR</t> analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01
    Qrt Pcr, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 92/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Toyobo qrt pcr
    Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for <t>qRT-PCR</t> analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01
    Qrt Pcr, supplied by Toyobo, used in various techniques. Bioz Stars score: 94/100, based on 494 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche lightcycler 480 qrt pcr instrument
    Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for <t>qRT-PCR</t> analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01
    Lightcycler 480 Qrt Pcr Instrument, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TransGen biotech co transstart green qrt pcr supermix
    Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for <t>qRT-PCR</t> analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01
    Transstart Green Qrt Pcr Supermix, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Kapa Biosystems qrt pcr reaction
    Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for <t>qRT-PCR</t> analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01
    Qrt Pcr Reaction, supplied by Kapa Biosystems, used in various techniques. Bioz Stars score: 90/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher qrt pcr analysis
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Qrt Pcr Analysis, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 11244 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    TaKaRa real time quantitative pcr qrt pcr assays
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Real Time Quantitative Pcr Qrt Pcr Assays, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher superscript iii first strand synthesis supermix for qrt pcr
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Superscript Iii First Strand Synthesis Supermix For Qrt Pcr, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher quantstudio dx
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Quantstudio Dx, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher luminaris color higreen qpcr master mix
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Luminaris Color Higreen Qpcr Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 523 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche light cycler detection system
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Light Cycler Detection System, supplied by Roche, used in various techniques. Bioz Stars score: 90/100, based on 1151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr select master mix
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Sybr Select Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 10306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Qiagen quantitect sybr green rt pcr kit
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Quantitect Sybr Green Rt Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 8359 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher sybr green master mix
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Sybr Green Master Mix, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 33260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher oligo dt 18 primer
    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through <t>qRT-PCR</t> was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.
    Oligo Dt 18 Primer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1030 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    TaFT3 homoeolog-specific qRT-PCR gene expression in hexaploid wheat cultivars Cadenza (A–C) and Chinese Spring (D–F) and tetraploid wheat cultivar Kronos (G,H) grown under LD (white circles) and SD (black circles) photoperiods . Leaf samples for expression analyses were harvested at 0, 1, 2, 3 and 4 weeks. qRT-PCR TaFT3 expression data is normalized against four control genes ( ACTIN, UBIQUITIN, GAPDH, EF1A ). ± 1 standard error of the mean (SEM) indicated.

    Journal: Frontiers in Plant Science

    Article Title: Systematic Investigation of FLOWERING LOCUS T-Like Poaceae Gene Families Identifies the Short-Day Expressed Flowering Pathway Gene, TaFT3 in Wheat (Triticum aestivum L.)

    doi: 10.3389/fpls.2016.00857

    Figure Lengend Snippet: TaFT3 homoeolog-specific qRT-PCR gene expression in hexaploid wheat cultivars Cadenza (A–C) and Chinese Spring (D–F) and tetraploid wheat cultivar Kronos (G,H) grown under LD (white circles) and SD (black circles) photoperiods . Leaf samples for expression analyses were harvested at 0, 1, 2, 3 and 4 weeks. qRT-PCR TaFT3 expression data is normalized against four control genes ( ACTIN, UBIQUITIN, GAPDH, EF1A ). ± 1 standard error of the mean (SEM) indicated.

    Article Snippet: All qRT-PCR reactions were undertaken using SYBR MM (Roche), and carried out in total reaction volumes of 10 μl using a Light Cycler 480 (Roche) with the following cycling conditions: 95°C 5 mins; 45 cycles of 95°C for 10 s, 60°C for 15 s, 72°C for 30 s, cooling 60°C; melt curve was carried out by ramping 0.11°C/s from 60 to 95°C, with 5 readings taken per second.

    Techniques: Quantitative RT-PCR, Expressing

    Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for qRT-PCR analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01

    Journal: Frontiers in Plant Science

    Article Title: Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

    doi: 10.3389/fpls.2017.01436

    Figure Lengend Snippet: Expression profiles of GmNRAMP genes under nitrogen, phosphorus or potassium deficiency conditions. Ten-day-old soybean seedlings were subjected to nitrogen (LN), phosphorous (LP), or potassium (LK) deficiency conditions for 14 days, during which time obvious nutrient deficiency symptoms developed. Leaves and roots were separately harvested for qRT-PCR analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2 −ΔCt values. Differential expression was determined for four biological replicates in FDR adjusted t -tests of 2 −ΔCt values from nutrient deficient tissues vs. corresponding control tissues. The heat map displays 2 −ΔΔCt values to show relative expression of nutrient deficient samples vs. controls, with significant differences between treatment and control marked as follows, * 0.01

    Article Snippet: In addition, the specific primers for nutrient deficiency responsive genes were also designed and listed in Table . qRT-PCR reactions were carried out in a CFX connect Real-Time PCR Detection System (Bio-Rad, Hercules, USA) with SYBR® Premix Ex Taq™ II (TaKaRa, Tokyo, Japan).

    Techniques: Expressing, Quantitative RT-PCR

    Expression of GmNRAMPs in response to Fe or S deficiency. Ten-day-old soybean seedlings were subjected to Fe or S deficiency for 14 days. Leaves and roots were separately harvested for qRT-PCR analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2- ΔCt values. Each bar is the mean of four biological replicates with standard error. “ * ” indicates significant differences between soybean without and with rhizobia inoculation in young leaves, stems, and roots in one-way analysis of variance, * P

    Journal: Frontiers in Plant Science

    Article Title: Genome-Wide Identification and Expression Analysis of NRAMP Family Genes in Soybean (Glycine Max L.)

    doi: 10.3389/fpls.2017.01436

    Figure Lengend Snippet: Expression of GmNRAMPs in response to Fe or S deficiency. Ten-day-old soybean seedlings were subjected to Fe or S deficiency for 14 days. Leaves and roots were separately harvested for qRT-PCR analysis. Fold-changes of GmNRAMP gene expression were normalized against the reference gene TefS1 using 2- ΔCt values. Each bar is the mean of four biological replicates with standard error. “ * ” indicates significant differences between soybean without and with rhizobia inoculation in young leaves, stems, and roots in one-way analysis of variance, * P

    Article Snippet: In addition, the specific primers for nutrient deficiency responsive genes were also designed and listed in Table . qRT-PCR reactions were carried out in a CFX connect Real-Time PCR Detection System (Bio-Rad, Hercules, USA) with SYBR® Premix Ex Taq™ II (TaKaRa, Tokyo, Japan).

    Techniques: Expressing, Quantitative RT-PCR

    Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through qRT-PCR was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.

    Journal: Journal of Insect Science

    Article Title: Effect of Winning Experience on Aggression Involving Dangerous Fighting Behavior in Anastatus disparis (Hymenoptera: Eupelmidae)

    doi: 10.1093/jisesa/ieaa038

    Figure Lengend Snippet: Expression of annotated genes involved in sugar transporter (A and B) and dopamine synthesis (C). The expression of genes determined through qRT-PCR was calculated by the 2 −ΔΔCt method using the housekeeping gene EF1A as a reference to eliminate sample-to-sample variations in the initial cDNA samples. The error bars indicate standard errors. The same letter on the column indicates no significant difference; however, different letters indicate significant difference.

    Article Snippet: SYBR Green Real-Time PCR Master Mix (TaKaRa) was used for qRT-PCR analysis (ABI StepOne Plus; USA) following the manufacturer’s protocols.

    Techniques: Expressing, Quantitative RT-PCR