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  • 86
    Becton Dickinson edta tubes
    Study 3: Comparison of PBMC isolation methods and SMN measures from various blood fractions. Due to the variability in SMN signal and protein concentrations seen with <t>CPT</t> tube PBMC isolation, other methods were explored using the four subjects from Study 2. A : PBMC yield was greatest in samples collected with <t>EDTA</t> tubes and subsequent Lymphoprep gradient separation, and showed no statistically significant changes with isolation delays of up to 24 h. B : Total soluble protein tended to increase with 24 h delays in samples collected by EDTA tube and isolated by CPT tubes, while there was no obvious change in protein concentrations in EDTA/Lymphoprep processing. C : SMN as measured by total protein tended to decrease with isolation delays with EDTA/CPT processing. SMN signals were similar with delays up to 24 h with EDTA/Lymphoprep processing. D : SMN by PBMC counts was variable for both EDTA/CPT and EDTA/Lymphoprep processing methods. However, the EDTA/Lymphoprep values were generally overlapping and did not appear to decrease from the no-delay timepoint (t = 0). In Figure 3 body of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median.
    Edta Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta tubes/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta tubes - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson edta vacutainer tubes
    Analysis of circulating lymphocytes following influenza virus infection. For each virus, three to five ferrets were inoculated i.n. with 1 × 10 6 PFU. Blood was collected on days 3 (A), 7 (B), and 19 (C) p.i. into <t>EDTA</t> <t>Vacutainer</t> tubes and was
    Edta Vacutainer Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta vacutainer tubes/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta vacutainer tubes - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Sarstedt edta tubes
    Analysis of circulating lymphocytes following influenza virus infection. For each virus, three to five ferrets were inoculated i.n. with 1 × 10 6 PFU. Blood was collected on days 3 (A), 7 (B), and 19 (C) p.i. into <t>EDTA</t> <t>Vacutainer</t> tubes and was
    Edta Tubes, supplied by Sarstedt, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta tubes/product/Sarstedt
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta tubes - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

    86
    Becton Dickinson edta coated tubes
    Analysis of circulating lymphocytes following influenza virus infection. For each virus, three to five ferrets were inoculated i.n. with 1 × 10 6 PFU. Blood was collected on days 3 (A), 7 (B), and 19 (C) p.i. into <t>EDTA</t> <t>Vacutainer</t> tubes and was
    Edta Coated Tubes, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta coated tubes/product/Becton Dickinson
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    edta coated tubes - by Bioz Stars, 2021-05
    86/100 stars
      Buy from Supplier

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    Study 3: Comparison of PBMC isolation methods and SMN measures from various blood fractions. Due to the variability in SMN signal and protein concentrations seen with CPT tube PBMC isolation, other methods were explored using the four subjects from Study 2. A : PBMC yield was greatest in samples collected with EDTA tubes and subsequent Lymphoprep gradient separation, and showed no statistically significant changes with isolation delays of up to 24 h. B : Total soluble protein tended to increase with 24 h delays in samples collected by EDTA tube and isolated by CPT tubes, while there was no obvious change in protein concentrations in EDTA/Lymphoprep processing. C : SMN as measured by total protein tended to decrease with isolation delays with EDTA/CPT processing. SMN signals were similar with delays up to 24 h with EDTA/Lymphoprep processing. D : SMN by PBMC counts was variable for both EDTA/CPT and EDTA/Lymphoprep processing methods. However, the EDTA/Lymphoprep values were generally overlapping and did not appear to decrease from the no-delay timepoint (t = 0). In Figure 3 body of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median.

    Journal: PLoS ONE

    Article Title: Evaluation of Peripheral Blood Mononuclear Cell Processing and Analysis for Survival Motor Neuron Protein

    doi: 10.1371/journal.pone.0050763

    Figure Lengend Snippet: Study 3: Comparison of PBMC isolation methods and SMN measures from various blood fractions. Due to the variability in SMN signal and protein concentrations seen with CPT tube PBMC isolation, other methods were explored using the four subjects from Study 2. A : PBMC yield was greatest in samples collected with EDTA tubes and subsequent Lymphoprep gradient separation, and showed no statistically significant changes with isolation delays of up to 24 h. B : Total soluble protein tended to increase with 24 h delays in samples collected by EDTA tube and isolated by CPT tubes, while there was no obvious change in protein concentrations in EDTA/Lymphoprep processing. C : SMN as measured by total protein tended to decrease with isolation delays with EDTA/CPT processing. SMN signals were similar with delays up to 24 h with EDTA/Lymphoprep processing. D : SMN by PBMC counts was variable for both EDTA/CPT and EDTA/Lymphoprep processing methods. However, the EDTA/Lymphoprep values were generally overlapping and did not appear to decrease from the no-delay timepoint (t = 0). In Figure 3 body of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median.

    Article Snippet: Whole blood samples at the Jasper Clinic were collected in BD CPT tubes (#362761, Studies 1–3), EDTA tubes (#22-040-67, Fisher, Pittsburgh, PA in Studies 3–5, and SMAF-001) or ACD tubes (#363083, BD, Franklin Lakes, NJ in Study 5).

    Techniques: Isolation, Cycling Probe Technology

    Analysis of circulating lymphocytes following influenza virus infection. For each virus, three to five ferrets were inoculated i.n. with 1 × 10 6 PFU. Blood was collected on days 3 (A), 7 (B), and 19 (C) p.i. into EDTA Vacutainer tubes and was

    Journal: Journal of Virology

    Article Title: PB1-F2 Expression by the 2009 Pandemic H1N1 Influenza Virus Has Minimal Impact on Virulence in Animal Models ▿

    doi: 10.1128/JVI.02717-09

    Figure Lengend Snippet: Analysis of circulating lymphocytes following influenza virus infection. For each virus, three to five ferrets were inoculated i.n. with 1 × 10 6 PFU. Blood was collected on days 3 (A), 7 (B), and 19 (C) p.i. into EDTA Vacutainer tubes and was

    Article Snippet: On days zero, 3, 7, and 19 p.i., blood was collected in EDTA Vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) from 3 to 5 inoculated ferrets from each virus group.

    Techniques: Infection