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  • 99
    Thermo Fisher edta
    Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 16224 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore protease inhibitor cocktail edta free
    Protease Inhibitor Cocktail Edta Free, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Roche edta free buffer
    Edta Free Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche complete edta free buffer
    Complete Edta Free Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche edta free lysis buffer
    Edta Free Lysis Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    89
    Millipore enzyme free edta disassociation buffer
    Enzyme Free Edta Disassociation Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche edta free protease inhibitor buffer
    Edta Free Protease Inhibitor Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 89/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson enzyme free edta buffer
    Enzyme Free Edta Buffer, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche edta free complete lysis m buffer
    Edta Free Complete Lysis M Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche completetm lysis m edta free buffer
    Completetm Lysis M Edta Free Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Millipore nuclease free tris edta te buffer
    Nuclease Free Tris Edta Te Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Roche complete mini edta free buffer
    Complete Mini Edta Free Buffer, supplied by Roche, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Thermo Fisher t per edta free lysis buffer
    T Per Edta Free Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher rnase free tris edta te buffer
    Rnase Free Tris Edta Te Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    Thermo Fisher tper edta free lysis buffer
    Tper Edta Free Lysis Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 84/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Roche ripa buffer plus protease inhibitor cocktail edta free
    Ripa Buffer Plus Protease Inhibitor Cocktail Edta Free, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Roche cytoskeletal csk buffer buffer containing edta free protease inhibitor tablets
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Cytoskeletal Csk Buffer Buffer Containing Edta Free Protease Inhibitor Tablets, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Millipore binding buffer edta
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Binding Buffer Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 207 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher protease free phosphate buffered saline edetic acid solution
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Protease Free Phosphate Buffered Saline Edetic Acid Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Abcam protease inhibitor free buffer
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Protease Inhibitor Free Buffer, supplied by Abcam, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Roche edta free
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Edta Free, supplied by Roche, used in various techniques. Bioz Stars score: 99/100, based on 2401 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    Thermo Fisher free ringer buffer
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Free Ringer Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    87
    Thermo Fisher pbs edta enzyme free dissociation buffer
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Pbs Edta Enzyme Free Dissociation Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 87/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    80
    Millipore sterile rnase free tris edta buffer
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Sterile Rnase Free Tris Edta Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    97
    Roche complete edta free
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Complete Edta Free, supplied by Roche, used in various techniques. Bioz Stars score: 97/100, based on 744 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Millipore dnase rnase free tris edta buffer
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    Dnase Rnase Free Tris Edta Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 86/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    96
    Roche l buffer edta free complete protease inhibitor cocktail
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
    L Buffer Edta Free Complete Protease Inhibitor Cocktail, supplied by Roche, used in various techniques. Bioz Stars score: 96/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Covidien edta solution
    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
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    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
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    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing <t>cytoskeletal</t> buffer <t>(CSK),</t> 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.
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    Image Search Results


    Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing cytoskeletal buffer (CSK), 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.

    Journal: Cell Cycle

    Article Title: CIZ1-F, an alternatively spliced variant of the DNA replication protein CIZ1 with distinct expression and localisation, is overrepresented in early stage common solid tumours

    doi: 10.1080/15384101.2018.1526600

    Figure Lengend Snippet: Nuclear CIZ1-F resists extraction of chromatin but not RNA. ]. Immunofluorescence with purified antibody raised to the CIZ1-F ARF (red) shows absence of Xi-localization. DNA is stained with Hoechst33258 (blue). Nuclei in white boxes are enlarged in (B). (C) Overview of nuclear matrix (NM) extraction procedure. MCF-7 cells were serially extracted with 1) detergent-containing cytoskeletal buffer (CSK), 2) detergent-containing CSK supplemented with 0.5 M NaCl (mock extraction), 3) DNase 1, or 4) RNase, or 5) both enzymes (see methods). (D) MCF-7 cells subjected to the treatments described under (C) were counterstained with Hoechst33258 to control for removal of chromatin (blue), and probed with purified polyclonal anti-CIZ1-F or replication domain (CIZ1-RD) antibody 1794 (red). CIZ1-RD resists all treatments, while CIZ1-F is sensitive to extraction of RNA, and is also dramatically revealed by removal of chromatin. Bar is 10 microns. (E) Quantification of the fluorescence intensities in (D), shown after subtraction of background signal and expressed relative to detergent treated cells. Number of cells quantified ≥ 70 per condition. Numbers refer to the treatment conditions listed above. (F) Enhanced and enlarged images of the indicated nuclei i-iv from (D), showing detergent and DNase-resistant nuclear fractions. (G) Interpretation of the data showing the dependency of CIZ1-F on RNA for nuclear retention, compared to full resistance of CIZ1-RD.

    Article Snippet: For immunofluorescence, cells grown on glass coverslips were transferred into PBS, washed with cytoskeletal (CSK) buffer (containing EDTA-free protease inhibitor tablets [cat. 05056489001; F Hoffmann-La Roche Ltd, Sigma Aldrich] and 1 mM DTT) with or without 0.1% Triton X-100 [ ] for 30 s, then transferred to PBS and fixed for 20 min in 4% paraformaldehyde in PBS.

    Techniques: Immunofluorescence, Purification, Staining, Fluorescence