edta Millipore Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99
    Millipore edta
    Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 32373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Millipore
    Average 99 stars, based on 32373 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    98
    Thermo Fisher edta
    Edta, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 16222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Thermo Fisher
    Average 98 stars, based on 16222 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2020-01
    98/100 stars
      Buy from Supplier

    81
    Millipore edta edta
    PCT concentration in healthy volunteers. Average PCT concentration in human <t>K2</t> EDTA plasma and serum was 15.8 pg·mL −1 and 10.6 pg·mL −1 , respectively. The PCT concentration of all 86 analyzed samples was above the LLoQ of the Simoa PCT assay. Data are presented as scatter plots. The middle line represents the mean value of a group, the dotted line the LLoQ of the B·R·A·H·M·S PCT-sensitive Kryptor assay, and the dashed line the LLoQ of the Simoa PCT assay.
    Edta Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 81/100, based on 700 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta edta/product/Millipore
    Average 81 stars, based on 700 article reviews
    Price from $9.99 to $1999.99
    edta edta - by Bioz Stars, 2020-01
    81/100 stars
      Buy from Supplier

    97
    Merck KGaA edta
    PCT concentration in healthy volunteers. Average PCT concentration in human <t>K2</t> EDTA plasma and serum was 15.8 pg·mL −1 and 10.6 pg·mL −1 , respectively. The PCT concentration of all 86 analyzed samples was above the LLoQ of the Simoa PCT assay. Data are presented as scatter plots. The middle line represents the mean value of a group, the dotted line the LLoQ of the B·R·A·H·M·S PCT-sensitive Kryptor assay, and the dashed line the LLoQ of the Simoa PCT assay.
    Edta, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 729 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta/product/Merck KGaA
    Average 97 stars, based on 729 article reviews
    Price from $9.99 to $1999.99
    edta - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    77
    Millipore edta disodium salt edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Disodium Salt Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta disodium salt edta/product/Millipore
    Average 77 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    edta disodium salt edta - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    79
    Millipore edta free complete
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Free Complete, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta free complete/product/Millipore
    Average 79 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    edta free complete - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    98
    Millipore trypsin edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Trypsin Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 98/100, based on 7066 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta/product/Millipore
    Average 98 stars, based on 7066 article reviews
    Price from $9.99 to $1999.99
    trypsin edta - by Bioz Stars, 2020-01
    98/100 stars
      Buy from Supplier

    97
    Merck KGaA trypsin edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Trypsin Edta, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 97/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta/product/Merck KGaA
    Average 97 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
    trypsin edta - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    89
    Millipore 1 mm edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    1 Mm Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 89/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1 mm edta/product/Millipore
    Average 89 stars, based on 51 article reviews
    Price from $9.99 to $1999.99
    1 mm edta - by Bioz Stars, 2020-01
    89/100 stars
      Buy from Supplier

    87
    Millipore edta dipotassium salt
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Dipotassium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 87/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta dipotassium salt/product/Millipore
    Average 87 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    edta dipotassium salt - by Bioz Stars, 2020-01
    87/100 stars
      Buy from Supplier

    77
    Millipore ethylenediaminetriacetic acid edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Ethylenediaminetriacetic Acid Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 77/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ethylenediaminetriacetic acid edta/product/Millipore
    Average 77 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ethylenediaminetriacetic acid edta - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    79
    Millipore ca2 chelator edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Ca2 Chelator Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ca2 chelator edta/product/Millipore
    Average 79 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    ca2 chelator edta - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    84
    Millipore edta free acid
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Free Acid, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta free acid/product/Millipore
    Average 84 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    edta free acid - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    91
    Millipore edta tetrasodium salt
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Tetrasodium Salt, supplied by Millipore, used in various techniques. Bioz Stars score: 91/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta tetrasodium salt/product/Millipore
    Average 91 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    edta tetrasodium salt - by Bioz Stars, 2020-01
    91/100 stars
      Buy from Supplier

    78
    Millipore m fe edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    M Fe Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/m fe edta/product/Millipore
    Average 78 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    m fe edta - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    79
    Millipore trypsin edta 4
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Trypsin Edta 4, supplied by Millipore, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta 4/product/Millipore
    Average 79 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    trypsin edta 4 - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    82
    Millipore edta free trypsin
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Free Trypsin, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta free trypsin/product/Millipore
    Average 82 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    edta free trypsin - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

    78
    Millipore contaminant free edta powder
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Contaminant Free Edta Powder, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/contaminant free edta powder/product/Millipore
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    contaminant free edta powder - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    97
    Millipore tris edta buffer
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Tris Edta Buffer, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1021 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris edta buffer/product/Millipore
    Average 97 stars, based on 1021 article reviews
    Price from $9.99 to $1999.99
    tris edta buffer - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    84
    Millipore acid edta solution
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Acid Edta Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/acid edta solution/product/Millipore
    Average 84 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    acid edta solution - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    82
    Millipore edta aqueous solution
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Aqueous Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 82/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta aqueous solution/product/Millipore
    Average 82 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    edta aqueous solution - by Bioz Stars, 2020-01
    82/100 stars
      Buy from Supplier

    85
    Millipore edta free protease cocktail
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Edta Free Protease Cocktail, supplied by Millipore, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/edta free protease cocktail/product/Millipore
    Average 85 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    edta free protease cocktail - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    92
    Millipore tris acetate edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Tris Acetate Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris acetate edta/product/Millipore
    Average 92 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    tris acetate edta - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    84
    Millipore trypsin edta 1×
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Trypsin Edta 1×, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta 1×/product/Millipore
    Average 84 stars, based on 28 article reviews
    Price from $9.99 to $1999.99
    trypsin edta 1× - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    83
    Millipore na2 edta 2h2 o
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Na2 Edta 2h2 O, supplied by Millipore, used in various techniques. Bioz Stars score: 83/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/na2 edta 2h2 o/product/Millipore
    Average 83 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    na2 edta 2h2 o - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    88
    Millipore trypin edta solution
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Trypin Edta Solution, supplied by Millipore, used in various techniques. Bioz Stars score: 88/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypin edta solution/product/Millipore
    Average 88 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    trypin edta solution - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    84
    Millipore disodium edta 2h2 o
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Disodium Edta 2h2 O, supplied by Millipore, used in various techniques. Bioz Stars score: 84/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/disodium edta 2h2 o/product/Millipore
    Average 84 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    disodium edta 2h2 o - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    92
    Millipore 1x trypsin edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    1x Trypsin Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/1x trypsin edta/product/Millipore
    Average 92 stars, based on 25 article reviews
    Price from $9.99 to $1999.99
    1x trypsin edta - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    78
    Millipore trypsin edta 9x
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Trypsin Edta 9x, supplied by Millipore, used in various techniques. Bioz Stars score: 78/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trypsin edta 9x/product/Millipore
    Average 78 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    trypsin edta 9x - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    99
    Millipore tris borate edta
    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM <t>DTT,</t> and 1 mM <t>EDTA</t> (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.
    Tris Borate Edta, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris borate edta/product/Millipore
    Average 99 stars, based on 159 article reviews
    Price from $9.99 to $1999.99
    tris borate edta - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    Image Search Results


    PCT concentration in healthy volunteers. Average PCT concentration in human K2 EDTA plasma and serum was 15.8 pg·mL −1 and 10.6 pg·mL −1 , respectively. The PCT concentration of all 86 analyzed samples was above the LLoQ of the Simoa PCT assay. Data are presented as scatter plots. The middle line represents the mean value of a group, the dotted line the LLoQ of the B·R·A·H·M·S PCT-sensitive Kryptor assay, and the dashed line the LLoQ of the Simoa PCT assay.

    Journal: High-Throughput

    Article Title: Development and Validation of an Ultrasensitive Procalcitonin Sandwich Immunoassay

    doi: 10.3390/ht6040018

    Figure Lengend Snippet: PCT concentration in healthy volunteers. Average PCT concentration in human K2 EDTA plasma and serum was 15.8 pg·mL −1 and 10.6 pg·mL −1 , respectively. The PCT concentration of all 86 analyzed samples was above the LLoQ of the Simoa PCT assay. Data are presented as scatter plots. The middle line represents the mean value of a group, the dotted line the LLoQ of the B·R·A·H·M·S PCT-sensitive Kryptor assay, and the dashed line the LLoQ of the Simoa PCT assay.

    Article Snippet: The calibrator/sample diluent was PBS supplemented with 4% (w/v ) bovine serum albumin (Merck), 0.25% Tween-20 (Sigma Aldrich), and 0.3% (w/v ) K2 EDTA (Sigma Aldrich).

    Techniques: Concentration Assay

    Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.

    Journal: Biochemistry

    Article Title: Acetylation of Gly1 and Lys2 Promotes Aggregation of Human γD-Crystallin

    doi: 10.1021/bi501004y

    Figure Lengend Snippet: Acetylation exposes additional hydrophobic sites at the surface of γD-crystallin. The concentrations of the protein samples and bis-ANS were 2.5 μM and 10 μM, respectively. All samples were prepared in 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0). The fluorescence spectrum of bis-ANS bound to different samples was recorded from 450 to 600 nm at 25 °C. The excitation wavelength was 390 nm.

    Article Snippet: Materials and Methods Dithiothreitol (DTT); 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt (bis-ANS); guanidium hydrochloride (GdnHCl); and ethylenediaminetetraacetic acid, disodium salt dehydrate (EDTA) were obtained from Sigma Chemical Co. (St. Louis, MO).

    Techniques: Fluorescence

    Acetylation perturbed only the tertiary structure of γD-crystallin. (A) Far-UV CD spectra of nonacetylated and acetylated human γD-crystallin. (B) Near-UV CD spectra of nonacetylated and acetylated human γD-crystallin. The concentrations of the protein samples used in far- and near-UV CD were 0.2 and 1.0 mg/mL, respectively. (C) Intrinsic tryptophan fluorescence spectra of nonacetylated and acetylated human γD-crystallin (0.025 mg/mL) were recorded from 310 to 400 nm. The excitation wavelength was 295 nm. Excitation and emission slit widths were 5 nm each. The data were collected at a 0.5 nm wavelength resolution. All assays were performed in 10 mM phosphate buffer containing 1 mM EDTA and 5 mM DTT (pH 7.0) at 25 °C.

    Journal: Biochemistry

    Article Title: Acetylation of Gly1 and Lys2 Promotes Aggregation of Human γD-Crystallin

    doi: 10.1021/bi501004y

    Figure Lengend Snippet: Acetylation perturbed only the tertiary structure of γD-crystallin. (A) Far-UV CD spectra of nonacetylated and acetylated human γD-crystallin. (B) Near-UV CD spectra of nonacetylated and acetylated human γD-crystallin. The concentrations of the protein samples used in far- and near-UV CD were 0.2 and 1.0 mg/mL, respectively. (C) Intrinsic tryptophan fluorescence spectra of nonacetylated and acetylated human γD-crystallin (0.025 mg/mL) were recorded from 310 to 400 nm. The excitation wavelength was 295 nm. Excitation and emission slit widths were 5 nm each. The data were collected at a 0.5 nm wavelength resolution. All assays were performed in 10 mM phosphate buffer containing 1 mM EDTA and 5 mM DTT (pH 7.0) at 25 °C.

    Article Snippet: Materials and Methods Dithiothreitol (DTT); 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt (bis-ANS); guanidium hydrochloride (GdnHCl); and ethylenediaminetetraacetic acid, disodium salt dehydrate (EDTA) were obtained from Sigma Chemical Co. (St. Louis, MO).

    Techniques: Fluorescence

    Acetylation alters the unfolding and refolding of γD-crystallin. Productive kinetic unfolding data of nonacetylated and acetylated human γD-crystallin (A). For unfolding, nonacetylated proteins (0.1 mg/mL) were diluted into 5.5 M GdnHCl, 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0) at 25 °C. Changes in the fluorescence intensity at 355 nm were monitored over time using an excitation wavelength of 295 nm. The final protein concentration in the unfolding buffer was 0.01 mg/mL. Unfolding time-course profiles (B) of both proteins were fitted with double and single exponentials, respectively, as indicated by solid lines. Productive kinetic refolding data of nonacetylated and acetylated human γD-crystallin (C). The protein (0.1 mg/mL) was initially unfolded in 5.5 M GdnHCl and diluted into 10 mM phosphate, 5 mM DTT, and 1 mM EDTA (pH 7.0) at 25 °C to yield a final GdnHCl concentration of 1.0 M. The final protein concentration was 0.01 mg/mL. Changes in the fluorescence intensity at 355 nm were monitored over time using an excitation wavelength of 295 nm. Both refolding time-course profiles were fitted with double exponentials, as indicated by solid lines (D).

    Journal: Biochemistry

    Article Title: Acetylation of Gly1 and Lys2 Promotes Aggregation of Human γD-Crystallin

    doi: 10.1021/bi501004y

    Figure Lengend Snippet: Acetylation alters the unfolding and refolding of γD-crystallin. Productive kinetic unfolding data of nonacetylated and acetylated human γD-crystallin (A). For unfolding, nonacetylated proteins (0.1 mg/mL) were diluted into 5.5 M GdnHCl, 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0) at 25 °C. Changes in the fluorescence intensity at 355 nm were monitored over time using an excitation wavelength of 295 nm. The final protein concentration in the unfolding buffer was 0.01 mg/mL. Unfolding time-course profiles (B) of both proteins were fitted with double and single exponentials, respectively, as indicated by solid lines. Productive kinetic refolding data of nonacetylated and acetylated human γD-crystallin (C). The protein (0.1 mg/mL) was initially unfolded in 5.5 M GdnHCl and diluted into 10 mM phosphate, 5 mM DTT, and 1 mM EDTA (pH 7.0) at 25 °C to yield a final GdnHCl concentration of 1.0 M. The final protein concentration was 0.01 mg/mL. Changes in the fluorescence intensity at 355 nm were monitored over time using an excitation wavelength of 295 nm. Both refolding time-course profiles were fitted with double exponentials, as indicated by solid lines (D).

    Article Snippet: Materials and Methods Dithiothreitol (DTT); 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt (bis-ANS); guanidium hydrochloride (GdnHCl); and ethylenediaminetetraacetic acid, disodium salt dehydrate (EDTA) were obtained from Sigma Chemical Co. (St. Louis, MO).

    Techniques: Fluorescence, Protein Concentration, Concentration Assay

    Acetylated γD-crystallin is structurally less stable than the nonacetylated γD-crystallin. Equilibrium GdnHCl unfolding profile for 0.025 mg/mL of nonacetylated and acetylated human γD-crystallin at 37 °C (A). The profile was normalized to a scale of 0–1. Symbols represent the experimental data points, and the solid lines represent the best fit according to the three-state model. The thermal stability of both proteins was evaluated by far-UV CD measurements (B). The temperature was controlled by a water bath, and the data were recorded at a given temperature after a 2 min equilibration. A protein concentration of 0.1 mg/mL in 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0) was used. The raw data were fitted to a two-state model, and the fitting results are represented by solid lines. The thermal stability of both proteins was evaluated by monitoring the intrinsic tryptophan fluorescence (C). The temperature was controlled by a water bath, and the data were recorded at a given temperature after a 2 min equilibration. A protein concentration of 0.025 mg/mL in 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0) was used. The raw data were fitted to a two-state model, and the fitting results are represented by solid lines. Potential energy estimation computed from the molecular dynamics simulation (D). The relative difference in the potential energy profiles indicate the stability of the macro-models in the order nonacetylated > K2-acetylated > G1- and K2-acetylated.

    Journal: Biochemistry

    Article Title: Acetylation of Gly1 and Lys2 Promotes Aggregation of Human γD-Crystallin

    doi: 10.1021/bi501004y

    Figure Lengend Snippet: Acetylated γD-crystallin is structurally less stable than the nonacetylated γD-crystallin. Equilibrium GdnHCl unfolding profile for 0.025 mg/mL of nonacetylated and acetylated human γD-crystallin at 37 °C (A). The profile was normalized to a scale of 0–1. Symbols represent the experimental data points, and the solid lines represent the best fit according to the three-state model. The thermal stability of both proteins was evaluated by far-UV CD measurements (B). The temperature was controlled by a water bath, and the data were recorded at a given temperature after a 2 min equilibration. A protein concentration of 0.1 mg/mL in 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0) was used. The raw data were fitted to a two-state model, and the fitting results are represented by solid lines. The thermal stability of both proteins was evaluated by monitoring the intrinsic tryptophan fluorescence (C). The temperature was controlled by a water bath, and the data were recorded at a given temperature after a 2 min equilibration. A protein concentration of 0.025 mg/mL in 10 mM phosphate buffer, 5 mM DTT, and 1 mM EDTA (pH 7.0) was used. The raw data were fitted to a two-state model, and the fitting results are represented by solid lines. Potential energy estimation computed from the molecular dynamics simulation (D). The relative difference in the potential energy profiles indicate the stability of the macro-models in the order nonacetylated > K2-acetylated > G1- and K2-acetylated.

    Article Snippet: Materials and Methods Dithiothreitol (DTT); 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt (bis-ANS); guanidium hydrochloride (GdnHCl); and ethylenediaminetetraacetic acid, disodium salt dehydrate (EDTA) were obtained from Sigma Chemical Co. (St. Louis, MO).

    Techniques: Protein Concentration, Fluorescence

    Acetylated γD-crystallin is more prone to thermal aggregation and less protected by α L -crystallin. (A) Time-course aggregation of nonacetylated and acetylated human γD-crystallin at 80 °C. The samples were prepared in 10 mM phosphate buffer containing 1 mM EDTA (pH 7.0). 1: Nonacetylated; 2: acetylated; 3: nonacetylated with 5 mM DTT; and 4: acetylated with 5 mM DTT. The protein concentration was 0.1 mg/mL. (B) Scattering values of both proteins during thermal aggregation after 1 h. (C) Time-course aggregation of nonacetylated and acetylated γD-crystallin at 80 °C in the presence/absence of α L -crystallin. The samples were prepared in 10 mM phosphate buffer containing 5 mM DTT and 1 mM EDTA (pH 7). The concentration of both γD-crystallin proteins was 0.1 mg/mL. 1: Nonacetylated; 2: acetylated; 3: α L -crystallin alone; 4: nonacetylated + α L -crystallin (1:1) (w/w); 5: acetylated + α L -crystallin (1:1) (w/w); 6: nonacetylated + α L -crystallin (1:0.75) (w/w); and 7: acetylated + α L -crystallin (1:0.75)(w/w). (D) Percent protection of nonacetylated and acetylated γD-crystallin by α L -crystallin during thermal aggregation after 1 h. Bars represent the means ± SD of three independent experiments. * p

    Journal: Biochemistry

    Article Title: Acetylation of Gly1 and Lys2 Promotes Aggregation of Human γD-Crystallin

    doi: 10.1021/bi501004y

    Figure Lengend Snippet: Acetylated γD-crystallin is more prone to thermal aggregation and less protected by α L -crystallin. (A) Time-course aggregation of nonacetylated and acetylated human γD-crystallin at 80 °C. The samples were prepared in 10 mM phosphate buffer containing 1 mM EDTA (pH 7.0). 1: Nonacetylated; 2: acetylated; 3: nonacetylated with 5 mM DTT; and 4: acetylated with 5 mM DTT. The protein concentration was 0.1 mg/mL. (B) Scattering values of both proteins during thermal aggregation after 1 h. (C) Time-course aggregation of nonacetylated and acetylated γD-crystallin at 80 °C in the presence/absence of α L -crystallin. The samples were prepared in 10 mM phosphate buffer containing 5 mM DTT and 1 mM EDTA (pH 7). The concentration of both γD-crystallin proteins was 0.1 mg/mL. 1: Nonacetylated; 2: acetylated; 3: α L -crystallin alone; 4: nonacetylated + α L -crystallin (1:1) (w/w); 5: acetylated + α L -crystallin (1:1) (w/w); 6: nonacetylated + α L -crystallin (1:0.75) (w/w); and 7: acetylated + α L -crystallin (1:0.75)(w/w). (D) Percent protection of nonacetylated and acetylated γD-crystallin by α L -crystallin during thermal aggregation after 1 h. Bars represent the means ± SD of three independent experiments. * p

    Article Snippet: Materials and Methods Dithiothreitol (DTT); 4,4′-dianilino-1,1′-binaphthyl-5,5′-disulfonic acid, dipotassium salt (bis-ANS); guanidium hydrochloride (GdnHCl); and ethylenediaminetetraacetic acid, disodium salt dehydrate (EDTA) were obtained from Sigma Chemical Co. (St. Louis, MO).

    Techniques: Protein Concentration, Concentration Assay