Journal: PLoS ONE

Article Title: Evaluation of Peripheral Blood Mononuclear Cell Processing and Analysis for Survival Motor Neuron Protein

doi: 10.1371/journal.pone.0050763

Figure Lengend Snippet: Study 3: Comparison of PBMC isolation methods and SMN measures from various blood fractions. Due to the variability in SMN signal and protein concentrations seen with CPT tube PBMC isolation, other methods were explored using the four subjects from Study 2. A : PBMC yield was greatest in samples collected with EDTA tubes and subsequent Lymphoprep gradient separation, and showed no statistically significant changes with isolation delays of up to 24 h. B : Total soluble protein tended to increase with 24 h delays in samples collected by EDTA tube and isolated by CPT tubes, while there was no obvious change in protein concentrations in EDTA/Lymphoprep processing. C : SMN as measured by total protein tended to decrease with isolation delays with EDTA/CPT processing. SMN signals were similar with delays up to 24 h with EDTA/Lymphoprep processing. D : SMN by PBMC counts was variable for both EDTA/CPT and EDTA/Lymphoprep processing methods. However, the EDTA/Lymphoprep values were generally overlapping and did not appear to decrease from the no-delay timepoint (t = 0). In Figure 3 body of the boxplots indicate the first and third quartiles, while the horizontal bar indicates the median.

Article Snippet: Whole blood samples at the Jasper Clinic were collected in BD CPT tubes (#362761, Studies 1–3), EDTA tubes (#22-040-67, Fisher, Pittsburgh, PA in Studies 3–5, and SMAF-001) or ACD tubes (#363083, BD, Franklin Lakes, NJ in Study 5).

Techniques: Isolation, Cycling Probe Technology

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: Aptamer-functionalized hybrid nanoparticle for the treatment of breast cancer

doi: 10.1016/j.ejpb.2017.01.011

Figure Lengend Snippet: Knockdown of P-gp in 4T1-R (top panel), SKBR-3 (middle panel) and MCF-7 (bottom panel) breast cancer cells with/without aptamer-labeled F31 nanoparticles compared to lipofectamine transfection. In both cases of lipofectamine and aptamer-labeled nanoparticle transfection, the cells were transfected with 100 pmol siRNA for 24h, and then the cells were scraped by using trypsin-EDTA, washed with PBS, pelleted and the expression of P-gp was measured by Western blot analysis.

Article Snippet: After treatment, the cells were scraped by using trypsin-EDTA, washed and resuspended in PBS and kept on ice until they were used to quantify the uptake of the nanoparticles by those cells by FACS (BD FACSCalibur).

Techniques: Labeling, Transfection, Expressing, Western Blot

Journal: European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V

Article Title: Aptamer-functionalized hybrid nanoparticle for the treatment of breast cancer

doi: 10.1016/j.ejpb.2017.01.011

Figure Lengend Snippet: Measurement of the expression of Her-2 (A.) and P-gp (B.) in different mouse ( i.e. 4T1-R and 4T1-S) and human breast cancer cells ( i.e. SKBR-3, MCF-7 and MDA MB-231) and human liver cancer cells ( i.e. Huh-7.5 and HepG2) by Western blot analysis. The cells were cultured for 24h and then they were scraped by using trypsin-EDTA, washed with PBS, pelleted and then equal amount of proteins was loaded to measure the expression of Her-2 (1:1000 dilution) and P-gp (1:1000 dilution) with β-actin (1:20,000 dilution) running as the loading control.

Article Snippet: After treatment, the cells were scraped by using trypsin-EDTA, washed and resuspended in PBS and kept on ice until they were used to quantify the uptake of the nanoparticles by those cells by FACS (BD FACSCalibur).

Techniques: Expressing, Multiple Displacement Amplification, Western Blot, Cell Culture

Journal: Nanoscale

Article Title: Antioxidant Chemistry of Graphene-Based Materials and its Role in Oxidation Protection Technology

doi: 10.1039/c4nr03275f

Figure Lengend Snippet: GO as a hydroxyl-radical scavenger. A: Addition of 100 ppm GO protects phenol red from oxidation by Fenton-generated · OH over 8 hrs. B: Addition of GO protects the spin-trap DMPO from oxidation by Fenton-generated · OH detected by EPR spectroscopy. The protective effect is concentration dependent over the range 0.1-90 ppm GO. C: Both phenol red monitoring and EPR were used to test if the GO-mediated protective effect is due to radical scavenging or Fenton suppression by Fe-binding. The protective effect of GO is still present when GO-Fe binding is suppressed by EDTA, confirming the importance of radical scavenging. D: GO also protects DMPO when · OH is generated sonochemically, in a non-Fenton assay, providing additional evidence for true radical scavenging. All experiments were done in PBS (pH 3.5).

Article Snippet: Hydrogen peroxide (H2 O2 ), phosphate buffered saline (PBS) (pH 7.4), and EDTA were purchased from Fisher Scientific Inc. (Pittsburgh, PA).

Techniques: Generated, Electron Paramagnetic Resonance, Spectroscopy, Concentration Assay, Binding Assay

Journal: Journal of Virology

Article Title: PB1-F2 Expression by the 2009 Pandemic H1N1 Influenza Virus Has Minimal Impact on Virulence in Animal Models ▿

doi: 10.1128/JVI.02717-09

Figure Lengend Snippet: Analysis of circulating lymphocytes following influenza virus infection. For each virus, three to five ferrets were inoculated i.n. with 1 × 10 6 PFU. Blood was collected on days 3 (A), 7 (B), and 19 (C) p.i. into EDTA Vacutainer tubes and was

Article Snippet: On days zero, 3, 7, and 19 p.i., blood was collected in EDTA Vacutainer tubes (Becton Dickinson, Franklin Lakes, NJ) from 3 to 5 inoculated ferrets from each virus group.

Techniques: Infection