edta Search Results


edta  (Dojindo Labs)
94
Dojindo Labs edta
Edta, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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clinimacs plus pbs edta buffer  (Miltenyi Biotec)
95
Miltenyi Biotec clinimacs plus pbs edta buffer
Clinimacs Plus Pbs Edta Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trypsin solution  (Beijing Solarbio Science)
96
Beijing Solarbio Science trypsin solution
Trypsin Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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goat serum albumin  (Santa Cruz Biotechnology)
91
Santa Cruz Biotechnology goat serum albumin
Goat Serum Albumin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dcode universal mutation detection system  (Bio-Rad)
96
Bio-Rad dcode universal mutation detection system
Dcode Universal Mutation Detection System, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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edta disodium dihydrate  (Dojindo Labs)
95
Dojindo Labs edta disodium dihydrate
Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) <t>MB/ethanol/EDTA/DMSO</t> in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the <t>indicated</t> <t>bacteria</t> (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.
Edta Disodium Dihydrate, supplied by Dojindo Labs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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rat plasma conjugase  (Rockland Immunochemicals)
90
Rockland Immunochemicals rat plasma conjugase
Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) <t>MB/ethanol/EDTA/DMSO</t> in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the <t>indicated</t> <t>bacteria</t> (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.
Rat Plasma Conjugase, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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tris edta buffer  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology tris edta buffer
Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) <t>MB/ethanol/EDTA/DMSO</t> in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the <t>indicated</t> <t>bacteria</t> (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.
Tris Edta Buffer, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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trypsin solution without edta  (Beyotime)
96
Beyotime trypsin solution without edta
Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) <t>MB/ethanol/EDTA/DMSO</t> in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the <t>indicated</t> <t>bacteria</t> (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.
Trypsin Solution Without Edta, supplied by Beyotime, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
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trypsin edta solution  (Beyotime)
97
Beyotime trypsin edta solution
Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) <t>MB/ethanol/EDTA/DMSO</t> in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the <t>indicated</t> <t>bacteria</t> (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.
Trypsin Edta Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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ethylenediaminetetraacetic acid  (Thermo Fisher)
99
Thermo Fisher ethylenediaminetetraacetic acid
Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) <t>MB/ethanol/EDTA/DMSO</t> in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the <t>indicated</t> <t>bacteria</t> (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.
Ethylenediaminetetraacetic Acid, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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10x tbe  (Bio-Rad)
93
Bio-Rad 10x tbe
Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) <t>MB/ethanol/EDTA/DMSO</t> in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the <t>indicated</t> <t>bacteria</t> (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.
10x Tbe, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) MB/ethanol/EDTA/DMSO in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the indicated bacteria (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.

Journal: Journal of Biomedical Optics

Article Title: Depth distributions of bacteria for the Pseudomonas aeruginosa-infected burn wounds treated by methylene blue-mediated photodynamic therapy in rats: effects of additives to photosensitizer

doi: 10.1117/1.jbo.27.1.018001

Figure Lengend Snippet: Fig. 3 Representative Gram-stained images of P. aeruginosa-infected burned skin sections of rats after five different treatments on day 2: (a) saline only (control), (b) MB/ethanol/EDTA/DMSO in the dark (PS-only), (c) aPDT with only MB (aPDT I), (d) aPDT with MB/ethanol/EDTA (aPDT II), and (e) aPDT with MB/ethanol/EDTA/DMSO (aPDT III). Three columns of images were taken at different magnifications for each group. The magnification of the images in the first column is 200×. A depth scale with box segments with a depth of 100 μm and a width of 120 μm is displayed, and the depth origin (0 μm) is set at the base of the epidermis (black dotted line). The negative depths (0 to −200 μm) represent the epidermis, including the stratum corneum and a region above it. The regions of 0 to ∼600 μm and ∼600 to ∼1400 μm correspond to the dermis and hypodermis, respectively. Higher magnification images of three representative segments, the epidermis (−100 to 0 μm), the uppermost region of the dermis (0 to 100 μm), and the deepest region of the dermis (500 to 600 μm) are shown in the second column. The magnified images of the black dashed line boxes in the second column are shown in the third column with the indicated bacteria (blue arrows). The regions defined with blue dashed lines indicate clustered bacteria with or without biofilms.

Article Snippet: Hydrophilic cationic PSs can bind with negatively charged lipopolysaccharide molecules in the outer membrane of Gram-negative bacteria.12 To the MB solution, 10-mM EDTA disodium dihydrate [2NA(EDTA.2Na)] (345-01865; Dojindo Laboratories Co., Kumamoto, Japan), 10% ethanol (057-00451; Fujifilm Wako Pure Chemical Co., Osaka, Japan), and 25% DMSO (043-07216; Fujifilm Wako Pure Chemical Co., Osaka, Japan) were added.

Techniques: Staining, Infection, Saline, Control, Bacteria