ecorv enzyme Search Results


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  • 99
    New England Biolabs enzymes ecor i
    Identification of the recombinant plasmid. (A) Detection of the housekeeping gene GAPDH. (B) Identification of the target gene by RT-PCR. (C) PCR product of the Fyn gene was subcloned into pMD18-T-Fyn and pEGFP-N1-Fyn. (D) Identification of pMD18-T-Fyn fragments produced by restriction enzyme digestion with Eco R I and Sma I. (E) Identification of pEGFP-N1-Fyn fragments generated by restriction enzyme digestion with Eco R I and Sma I. Lane 1, RT-PCR GAPDH product; Lane 2, negative control; Lane 3, RT-PCR Fyn product from brain; Lane 4, positive control; Lane 5, PCR product of pMD18-T-Fyn; Lane 6, PCR product of pEGFP-N1-Fyn; Lanes 7 and 9, positive control; Lanes 8 and 10, recombinant plasmid identification by digestion with <t>EcoR</t> I and Sma I (pMD18-T-Fyn, 2700 bp; pEGFP-N1, 4700 bp; Fyn, 1611 bp); Lane M, DNA marker.
    Enzymes Ecor I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymes ecor i/product/New England Biolabs
    Average 99 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    enzymes ecor i - by Bioz Stars, 2020-08
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    97
    Thermo Fisher restriction enzymes ecor i
    Identification of the recombinant plasmid. (A) Detection of the housekeeping gene GAPDH. (B) Identification of the target gene by RT-PCR. (C) PCR product of the Fyn gene was subcloned into pMD18-T-Fyn and pEGFP-N1-Fyn. (D) Identification of pMD18-T-Fyn fragments produced by restriction enzyme digestion with Eco R I and Sma I. (E) Identification of pEGFP-N1-Fyn fragments generated by restriction enzyme digestion with Eco R I and Sma I. Lane 1, RT-PCR GAPDH product; Lane 2, negative control; Lane 3, RT-PCR Fyn product from brain; Lane 4, positive control; Lane 5, PCR product of pMD18-T-Fyn; Lane 6, PCR product of pEGFP-N1-Fyn; Lanes 7 and 9, positive control; Lanes 8 and 10, recombinant plasmid identification by digestion with <t>EcoR</t> I and Sma I (pMD18-T-Fyn, 2700 bp; pEGFP-N1, 4700 bp; Fyn, 1611 bp); Lane M, DNA marker.
    Restriction Enzymes Ecor I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes ecor i/product/Thermo Fisher
    Average 97 stars, based on 33 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes ecor i - by Bioz Stars, 2020-08
    97/100 stars
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    86
    Thermo Fisher enzyme ecorv
    Electrophoresis of <t>RFLP</t> products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the <t>EcoRV</t> enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker
    Enzyme Ecorv, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme ecorv/product/Thermo Fisher
    Average 86 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    enzyme ecorv - by Bioz Stars, 2020-08
    86/100 stars
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    99
    TaKaRa ecorv enzymes
    Electrophoresis of <t>RFLP</t> products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the <t>EcoRV</t> enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker
    Ecorv Enzymes, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv enzymes/product/TaKaRa
    Average 99 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    ecorv enzymes - by Bioz Stars, 2020-08
    99/100 stars
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    88
    SibEnzyme restriction enzyme ecorv
    Electrophoresis of <t>RFLP</t> products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the <t>EcoRV</t> enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker
    Restriction Enzyme Ecorv, supplied by SibEnzyme, used in various techniques. Bioz Stars score: 88/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzyme ecorv/product/SibEnzyme
    Average 88 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    restriction enzyme ecorv - by Bioz Stars, 2020-08
    88/100 stars
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    88
    Promega ecori enzyme
    Electrophoresis of <t>RFLP</t> products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the <t>EcoRV</t> enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker
    Ecori Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecori enzyme/product/Promega
    Average 88 stars, based on 34 article reviews
    Price from $9.99 to $1999.99
    ecori enzyme - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    88
    Thermo Fisher ecorv restriction enzymes
    Electrophoresis of <t>RFLP</t> products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the <t>EcoRV</t> enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker
    Ecorv Restriction Enzymes, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecorv restriction enzymes/product/Thermo Fisher
    Average 88 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    ecorv restriction enzymes - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    Image Search Results


    Identification of the recombinant plasmid. (A) Detection of the housekeeping gene GAPDH. (B) Identification of the target gene by RT-PCR. (C) PCR product of the Fyn gene was subcloned into pMD18-T-Fyn and pEGFP-N1-Fyn. (D) Identification of pMD18-T-Fyn fragments produced by restriction enzyme digestion with Eco R I and Sma I. (E) Identification of pEGFP-N1-Fyn fragments generated by restriction enzyme digestion with Eco R I and Sma I. Lane 1, RT-PCR GAPDH product; Lane 2, negative control; Lane 3, RT-PCR Fyn product from brain; Lane 4, positive control; Lane 5, PCR product of pMD18-T-Fyn; Lane 6, PCR product of pEGFP-N1-Fyn; Lanes 7 and 9, positive control; Lanes 8 and 10, recombinant plasmid identification by digestion with EcoR I and Sma I (pMD18-T-Fyn, 2700 bp; pEGFP-N1, 4700 bp; Fyn, 1611 bp); Lane M, DNA marker.

    Journal: Journal of Veterinary Science

    Article Title: Mouse Fyn induces pseudopodium formation in Chinese hamster ovary cells

    doi: 10.4142/jvs.2014.15.1.111

    Figure Lengend Snippet: Identification of the recombinant plasmid. (A) Detection of the housekeeping gene GAPDH. (B) Identification of the target gene by RT-PCR. (C) PCR product of the Fyn gene was subcloned into pMD18-T-Fyn and pEGFP-N1-Fyn. (D) Identification of pMD18-T-Fyn fragments produced by restriction enzyme digestion with Eco R I and Sma I. (E) Identification of pEGFP-N1-Fyn fragments generated by restriction enzyme digestion with Eco R I and Sma I. Lane 1, RT-PCR GAPDH product; Lane 2, negative control; Lane 3, RT-PCR Fyn product from brain; Lane 4, positive control; Lane 5, PCR product of pMD18-T-Fyn; Lane 6, PCR product of pEGFP-N1-Fyn; Lanes 7 and 9, positive control; Lanes 8 and 10, recombinant plasmid identification by digestion with EcoR I and Sma I (pMD18-T-Fyn, 2700 bp; pEGFP-N1, 4700 bp; Fyn, 1611 bp); Lane M, DNA marker.

    Article Snippet: Restriction enzymes EcoR I and Sma I were purchased from New England Biolabs (USA).

    Techniques: Recombinant, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Produced, Generated, Negative Control, Positive Control, Marker

    Absence of activating Flt3 mutations in astrocytic gliomas. ( A ) Examples of GeneScan analyses of 6-FAM-labeled polymerase chain reaction fragments spanning the Flt3 region, in which internal tandem duplications can occur. The tumor samples analyzed show the wild-type peak at a mean fragment length of 330.07 ± 0.37 bp. No sample contained additional peaks around 400 bp or of any size compatible with internal tandem duplications up to a size of 500 bp. The double peak in the GBM sample merely reflects an overlap between two peaks with a distance of 0.85 bp, which can be interpreted as a polymerase chain reaction artifact. ( B ) Diffuse astrocytomas (A), anaplastic astrocytomas (AA), and glioblastomas (GBM) were screened for the D835 point mutation. Notes: An undigested product of the polymerase chain reaction (237 bp) served as a control (C) to indicate the expected fragment size and intensity in the event of a mutation. It comigrated with the indicated 234 bp fragment of the marker (M). It can be seen clearly that no tumor DNA contained a major fragment comigrating with the control, while two smaller fragments of identical size were observed in all tumor samples. Faint bands of higher molecular weight became visible by the highly sensitive silver stain. These bands most likely reflect annealing artifacts of the primers, given that the electrophoretic mobility was lower than that of the control. Alternatively, these fragments may represent traces of undigested DNA due to incomplete digestion of wild-type DNA by EcoRV. They do not indicate a heterozygous state (mutant and wild-type) of tumor cells, because 50% of mutated tumor DNA would have yielded band intensities similar to that observed in lane C. Abbreviations: GBM, glioblastoma multiforme; Flt3, fms-like tyrosine kinase-3; Flt3L, fms-like tyrosine kinase-3 ligand.

    Journal: OncoTargets and therapy

    Article Title: Abundance of Flt3 and its ligand in astrocytic tumors

    doi: 10.2147/OTT.S43114

    Figure Lengend Snippet: Absence of activating Flt3 mutations in astrocytic gliomas. ( A ) Examples of GeneScan analyses of 6-FAM-labeled polymerase chain reaction fragments spanning the Flt3 region, in which internal tandem duplications can occur. The tumor samples analyzed show the wild-type peak at a mean fragment length of 330.07 ± 0.37 bp. No sample contained additional peaks around 400 bp or of any size compatible with internal tandem duplications up to a size of 500 bp. The double peak in the GBM sample merely reflects an overlap between two peaks with a distance of 0.85 bp, which can be interpreted as a polymerase chain reaction artifact. ( B ) Diffuse astrocytomas (A), anaplastic astrocytomas (AA), and glioblastomas (GBM) were screened for the D835 point mutation. Notes: An undigested product of the polymerase chain reaction (237 bp) served as a control (C) to indicate the expected fragment size and intensity in the event of a mutation. It comigrated with the indicated 234 bp fragment of the marker (M). It can be seen clearly that no tumor DNA contained a major fragment comigrating with the control, while two smaller fragments of identical size were observed in all tumor samples. Faint bands of higher molecular weight became visible by the highly sensitive silver stain. These bands most likely reflect annealing artifacts of the primers, given that the electrophoretic mobility was lower than that of the control. Alternatively, these fragments may represent traces of undigested DNA due to incomplete digestion of wild-type DNA by EcoRV. They do not indicate a heterozygous state (mutant and wild-type) of tumor cells, because 50% of mutated tumor DNA would have yielded band intensities similar to that observed in lane C. Abbreviations: GBM, glioblastoma multiforme; Flt3, fms-like tyrosine kinase-3; Flt3L, fms-like tyrosine kinase-3 ligand.

    Article Snippet: The amplified DNA was cut using the EcoRV enzyme (New England Biolabs, Beverly, MA, USA) according to the manufacturer’s instructions.

    Techniques: Labeling, Polymerase Chain Reaction, Mutagenesis, Marker, Molecular Weight, Silver Staining

    Diagram of the portion of mitochondrial Cytochrome Oxidase I ( COI ) gene used to identify strain and the individual corn-strain (CS) haplotypes. The putative translational start site of the COI gene was arbitrarily designated as coordinate 0. Short block arrows indicate location and direction of the COI-893F and COI-1303R primers used for PCR amplification and DNA sequencing. Vertical lines within the COI gene identify polymorphic sites with the rice-strain (RS) specific EcoRV site identified. All polymorphic sites except 1287 were used to identify or confirm strain identity. The CS-h haplotypes were determined by the polymorphisms at site 1164 and 1287.

    Journal: Ecology and Evolution

    Article Title: Inferring the annual migration patterns of fall armyworm (Lepidoptera: Noctuidae) in the United States from mitochondrial haplotypes

    doi: 10.1002/ece3.268

    Figure Lengend Snippet: Diagram of the portion of mitochondrial Cytochrome Oxidase I ( COI ) gene used to identify strain and the individual corn-strain (CS) haplotypes. The putative translational start site of the COI gene was arbitrarily designated as coordinate 0. Short block arrows indicate location and direction of the COI-893F and COI-1303R primers used for PCR amplification and DNA sequencing. Vertical lines within the COI gene identify polymorphic sites with the rice-strain (RS) specific EcoRV site identified. All polymorphic sites except 1287 were used to identify or confirm strain identity. The CS-h haplotypes were determined by the polymorphisms at site 1164 and 1287.

    Article Snippet: Strain identification and DNA sequence analysis In each PCR, reaction mix (30 µL) was added 5 units of the restriction enzyme EcoRV (New England Biolabs) and 4 µL of the manufacturer recommended 10× restriction enzyme buffer (final volume taken to 40 µL with water).

    Techniques: Blocking Assay, Polymerase Chain Reaction, Amplification, DNA Sequencing

    In vitro analysis of NHEJ and HDR genome modification (arrows) mediated by sgRNA-Cas9 system in chicken cell lines. ( a ) Frequency (%) of NHEJ mutation mediated by KIAA1279, Cdkn1b and Mbd3 -targeting sgRNA-Cas9 system in chicken DF-1 cells by PCR and T7E1 assay. 1kM- 1 kbp DNA ladder, M- 100 bp DNA ladder. ( b ) Frequency (%) of NHEJ mutation mediated by KIAA1279 and Cdkn1b -targeting sgRNA-CRISPR/Cas9 system in chicken lymphoma B DT40 cells by PCR and T7E1 assay. ( c ) Representative gel from DF-1 cells transfected with the RET-targeting sgRNA-Cas9 and the ssODN showing efficient integration of the HDR-based BamHI and EcoRV sequence. The frequency of HDR is represented in percentages. 1-No sgRNA, 2- MEN2B sgRNA #1 plus ssODN, 3- MEN2B sgRNA #1 and #2 plus ssODN and 4- MEN2A/HSCR sgRNA 1 plus ssODN. ( d ) Representative gel for single cell clones derived from DF-1 cells transfected with the RET-targeting sgRNA-Cas9 and the ssODN for the MEN2B and MEN2A/HSCR HDR modifications respectively. The table shows the ratio of the monoallelic and biallelic HDR-based mutations detected with single cell clones and the overall efficiency in percentage: N = 19 for MEN2B and N = 12 for MEN2A/HSCR.

    Journal: Scientific Reports

    Article Title: CRISPR/Cas9 Targets Chicken Embryonic Somatic Cells In Vitro and In Vivo and generates Phenotypic Abnormalities

    doi: 10.1038/srep34524

    Figure Lengend Snippet: In vitro analysis of NHEJ and HDR genome modification (arrows) mediated by sgRNA-Cas9 system in chicken cell lines. ( a ) Frequency (%) of NHEJ mutation mediated by KIAA1279, Cdkn1b and Mbd3 -targeting sgRNA-Cas9 system in chicken DF-1 cells by PCR and T7E1 assay. 1kM- 1 kbp DNA ladder, M- 100 bp DNA ladder. ( b ) Frequency (%) of NHEJ mutation mediated by KIAA1279 and Cdkn1b -targeting sgRNA-CRISPR/Cas9 system in chicken lymphoma B DT40 cells by PCR and T7E1 assay. ( c ) Representative gel from DF-1 cells transfected with the RET-targeting sgRNA-Cas9 and the ssODN showing efficient integration of the HDR-based BamHI and EcoRV sequence. The frequency of HDR is represented in percentages. 1-No sgRNA, 2- MEN2B sgRNA #1 plus ssODN, 3- MEN2B sgRNA #1 and #2 plus ssODN and 4- MEN2A/HSCR sgRNA 1 plus ssODN. ( d ) Representative gel for single cell clones derived from DF-1 cells transfected with the RET-targeting sgRNA-Cas9 and the ssODN for the MEN2B and MEN2A/HSCR HDR modifications respectively. The table shows the ratio of the monoallelic and biallelic HDR-based mutations detected with single cell clones and the overall efficiency in percentage: N = 19 for MEN2B and N = 12 for MEN2A/HSCR.

    Article Snippet: For MEN2B HDR templates, the PCR products were digested in BamHI restriction enzyme (New England Biolabs, Ipswich, MA) and in EcoRV restriction enzyme (New England Biolabs, Ipswich, MA) for MEN2A/HSCR HDR experiments.

    Techniques: In Vitro, Non-Homologous End Joining, Modification, Mutagenesis, Polymerase Chain Reaction, CRISPR, Transfection, Sequencing, Clone Assay, Derivative Assay

    Electrophoresis of RFLP products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the EcoRV enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Lack of FLT3-TKD835 gene mutation in toxicity of sulfur mustard in Iranian veterans

    doi:

    Figure Lengend Snippet: Electrophoresis of RFLP products in patients who were exposed to sulfur mustard during the Iran-Iraq conflict. None of our patients (1, 2… 9) revealed FLT3-TKD mutation, because the EcoRV enzyme could digest the FLT3 gene sequences to two 68 bp and 46 bp pieces. In the positive control sample (uncut or P) the enzyme could not digest this and the sequence remained 114 bp. M: marker

    Article Snippet: After PCR and obtaining 114 bp sequences, for detecting D835 mutation based on RFLP procedure, products were incubated overnight at 37 °C with the EcoRV restriction enzyme (Fermentas).

    Techniques: Electrophoresis, Mutagenesis, Positive Control, Sequencing, Marker