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  • 93
    New England Biolabs nebuffer ecor i
    Nebuffer Ecor I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nebuffer ecor i/product/New England Biolabs
    Average 93 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
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    99
    Millipore ecor i
    Replication of transfected <t>DNA</t> in VAC-infected cells. (A) Southern blot of replicated circular plasmid and linear minichromosome. B-SC-1 cells were infected with VAC and 1 h later transfected with equal molar amounts (20 fmol) of super coiled pUC13 (sc pUC), pUC13 linearized by digestion with <t>EcoR</t> I (lin pUC), linear minichromosome containing pUC13 and 1.3 kbp viral telomeric sequences (lin mc). As a control, cells were mock infected and transfected with 20 fmol of linear minichromosome or 10 times that amount (200 fmol) of super coiled pUC13. At 24 h after infection or mock infection, cells were collected and total DNA extracted. Total DNA (2 μg) was digested with Dpn I subjected to agarose gel electrophoresis and analyzed by Southern blot hybridization using a 32 P-labeled pUC13 probe. Samples (0.5 fmol of lin pUC, 0.5 fmol of lin mc, 1 fmol sc pUC) of the DNA used for transfections (input DNA) were also analyzed. The positions of marker DNA (kbp) are shown on the left. (B) Real-time PCR of replicated plasmid. BS-C-1 cells were transfected with the plasmid p716 at 24 h prior to infection with VAC. At indicated hours post infection (hpi), cells were harvested and total DNA extracted. DNA was untreated or treated with Dpn I or Mbo I and analyzed by real-time PCR using primers specific to plasmid DNA. (C) Quantification of Southern blot. DNA described in panel (B) was digested with EcoR I prior to Mbo I or Dpn I treatment. The digested DNA samples were subjected to gel electrophoresis, transferred to a Nylon membrane, hybridized to a 32 P-labeled p716 probe, and the radioactivity quantified with a phosphoImager.
    Ecor I, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 451 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor i/product/Millipore
    Average 99 stars, based on 451 article reviews
    Price from $9.99 to $1999.99
    ecor i - by Bioz Stars, 2020-08
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    99
    TaKaRa ecor i
    Identification of the recombinant plasmid. (A) Detection of the housekeeping gene GAPDH. (B) Identification of the target gene by <t>RT-PCR.</t> (C) PCR product of the Fyn gene was subcloned into pMD18-T-Fyn and pEGFP-N1-Fyn. (D) Identification of pMD18-T-Fyn fragments produced by restriction enzyme digestion with Eco R I and Sma I. (E) Identification of pEGFP-N1-Fyn fragments generated by restriction enzyme digestion with Eco R I and Sma I. Lane 1, RT-PCR GAPDH product; Lane 2, negative control; Lane 3, RT-PCR Fyn product from brain; Lane 4, positive control; Lane 5, PCR product of pMD18-T-Fyn; Lane 6, PCR product of pEGFP-N1-Fyn; Lanes 7 and 9, positive control; Lanes 8 and 10, recombinant plasmid identification by digestion with <t>EcoR</t> I and Sma I (pMD18-T-Fyn, 2700 bp; pEGFP-N1, 4700 bp; Fyn, 1611 bp); Lane M, DNA marker.
    Ecor I, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1213 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor i/product/TaKaRa
    Average 99 stars, based on 1213 article reviews
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    94
    TaKaRa ecor v
    Electrophoresis of PCR product and pUCm-T-ZNRD1 digested with enzymes. Lane 1: PCR product; Lane 2, 3: Marker (200 bp); Lane 4: recombinant of pUCm-T-ZNRD1 cleaved by <t>EcoR</t> V and Xba I; Lane 5: recombinant of pUCm-T-ZNRD1.
    Ecor V, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 97 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v/product/TaKaRa
    Average 94 stars, based on 97 article reviews
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    93
    Millipore ecor v
    Electrophoresis of PCR product and pUCm-T-ZNRD1 digested with enzymes. Lane 1: PCR product; Lane 2, 3: Marker (200 bp); Lane 4: recombinant of pUCm-T-ZNRD1 cleaved by <t>EcoR</t> V and Xba I; Lane 5: recombinant of pUCm-T-ZNRD1.
    Ecor V, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 50 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor v/product/Millipore
    Average 93 stars, based on 50 article reviews
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    92
    Roche ecor i
    Electrophoresis of PCR product and pUCm-T-ZNRD1 digested with enzymes. Lane 1: PCR product; Lane 2, 3: Marker (200 bp); Lane 4: recombinant of pUCm-T-ZNRD1 cleaved by <t>EcoR</t> V and Xba I; Lane 5: recombinant of pUCm-T-ZNRD1.
    Ecor I, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 131 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor i/product/Roche
    Average 92 stars, based on 131 article reviews
    Price from $9.99 to $1999.99
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    93
    Promega ecor i
    Isolation of a segment of the gene np and construction of the expression vector. (A) Cloning representation of a gene coding for a segment of the protein NP from avian influenza virus subtype H7N1 comprising the aminoacids 49-375 (np 49-375 ) in the plasmid <t>pUC-18</t> and in the expression vector pET-28a. (B) Isolation of the gene np 49-375 by PCR. 1- Molecular weight marker (MWM) (pAdEasy digested with the enzyme Apa I), 2- DNA segment corresponding to the gene np 49-375 , 3- PCR reaction with primers and without template. (C) Electrophoresis in agarose gel (1%) of the restriction analysis for the plasmid pUC-np 49-375 . 1- MWM, 2- Plasmid pUC-np 49-375 undigested, 3- Plasmid pUC18 digested with the enzymes Nhe I/EcoR I, 4- Plasmid pUC-np 49-375 digested with the enzymes Nhe I/EcoR I. (D) Electrophoresis in agarose gel (1%) of the restriction analysis for the plasmid pET-28a-np 49-375 . 1- MWM, 2- Plasmid pET-28a-np 49-375 undigested, 3- Plasmid pET-28a digested with the enzymes Nhe I/EcoR I, 4- Plasmid pET-28a-np 49-375 digested with the enzymes Nhe I/EcoR I.
    Ecor I, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor i/product/Promega
    Average 93 stars, based on 391 article reviews
    Price from $9.99 to $1999.99
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    90
    Toyobo ecor i
    Genetic mapping and molecular analysis of the opsX mutant strain. (A) The solid triangle indicates the site of the Tn5 transposon insertion. Restriction sites for <t>EcoR</t> I (E) are also indicated. (B, C) PCR and Southern hybridization analysis for confirmation of transposon insertion in opsX : M1, 1-kb ladder; M2, lambda <t>DNA/</t> Hind III; lane 1, wild type; lanes 2 and 4, opsX mutant. The arrowhead indicates a single positive signal from the transposon (2,513-bp).
    Ecor I, supplied by Toyobo, used in various techniques. Bioz Stars score: 90/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor i/product/Toyobo
    Average 90 stars, based on 19 article reviews
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    ecor i - by Bioz Stars, 2020-08
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    90
    Thermo Fisher ecor 1
    Genetic mapping and molecular analysis of the opsX mutant strain. (A) The solid triangle indicates the site of the Tn5 transposon insertion. Restriction sites for <t>EcoR</t> I (E) are also indicated. (B, C) PCR and Southern hybridization analysis for confirmation of transposon insertion in opsX : M1, 1-kb ladder; M2, lambda <t>DNA/</t> Hind III; lane 1, wild type; lanes 2 and 4, opsX mutant. The arrowhead indicates a single positive signal from the transposon (2,513-bp).
    Ecor 1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecor 1/product/Thermo Fisher
    Average 90 stars, based on 41 article reviews
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    ecor 1 - by Bioz Stars, 2020-08
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    Image Search Results


    Replication of transfected DNA in VAC-infected cells. (A) Southern blot of replicated circular plasmid and linear minichromosome. B-SC-1 cells were infected with VAC and 1 h later transfected with equal molar amounts (20 fmol) of super coiled pUC13 (sc pUC), pUC13 linearized by digestion with EcoR I (lin pUC), linear minichromosome containing pUC13 and 1.3 kbp viral telomeric sequences (lin mc). As a control, cells were mock infected and transfected with 20 fmol of linear minichromosome or 10 times that amount (200 fmol) of super coiled pUC13. At 24 h after infection or mock infection, cells were collected and total DNA extracted. Total DNA (2 μg) was digested with Dpn I subjected to agarose gel electrophoresis and analyzed by Southern blot hybridization using a 32 P-labeled pUC13 probe. Samples (0.5 fmol of lin pUC, 0.5 fmol of lin mc, 1 fmol sc pUC) of the DNA used for transfections (input DNA) were also analyzed. The positions of marker DNA (kbp) are shown on the left. (B) Real-time PCR of replicated plasmid. BS-C-1 cells were transfected with the plasmid p716 at 24 h prior to infection with VAC. At indicated hours post infection (hpi), cells were harvested and total DNA extracted. DNA was untreated or treated with Dpn I or Mbo I and analyzed by real-time PCR using primers specific to plasmid DNA. (C) Quantification of Southern blot. DNA described in panel (B) was digested with EcoR I prior to Mbo I or Dpn I treatment. The digested DNA samples were subjected to gel electrophoresis, transferred to a Nylon membrane, hybridized to a 32 P-labeled p716 probe, and the radioactivity quantified with a phosphoImager.

    Journal: Virology Journal

    Article Title: Origin-independent plasmid replication occurs in vaccinia virus cytoplasmic factories and requires all five known poxvirus replication factors

    doi: 10.1186/1743-422X-2-23

    Figure Lengend Snippet: Replication of transfected DNA in VAC-infected cells. (A) Southern blot of replicated circular plasmid and linear minichromosome. B-SC-1 cells were infected with VAC and 1 h later transfected with equal molar amounts (20 fmol) of super coiled pUC13 (sc pUC), pUC13 linearized by digestion with EcoR I (lin pUC), linear minichromosome containing pUC13 and 1.3 kbp viral telomeric sequences (lin mc). As a control, cells were mock infected and transfected with 20 fmol of linear minichromosome or 10 times that amount (200 fmol) of super coiled pUC13. At 24 h after infection or mock infection, cells were collected and total DNA extracted. Total DNA (2 μg) was digested with Dpn I subjected to agarose gel electrophoresis and analyzed by Southern blot hybridization using a 32 P-labeled pUC13 probe. Samples (0.5 fmol of lin pUC, 0.5 fmol of lin mc, 1 fmol sc pUC) of the DNA used for transfections (input DNA) were also analyzed. The positions of marker DNA (kbp) are shown on the left. (B) Real-time PCR of replicated plasmid. BS-C-1 cells were transfected with the plasmid p716 at 24 h prior to infection with VAC. At indicated hours post infection (hpi), cells were harvested and total DNA extracted. DNA was untreated or treated with Dpn I or Mbo I and analyzed by real-time PCR using primers specific to plasmid DNA. (C) Quantification of Southern blot. DNA described in panel (B) was digested with EcoR I prior to Mbo I or Dpn I treatment. The digested DNA samples were subjected to gel electrophoresis, transferred to a Nylon membrane, hybridized to a 32 P-labeled p716 probe, and the radioactivity quantified with a phosphoImager.

    Article Snippet: Southern blotting DNA (2 μg) was digested with EcoR I and Dpn I or Mbo I, resolved on a 0.8% agarose gel, and transferred to Immobilon-Ny+ (Millipore) transfer membrane.

    Techniques: Transfection, Infection, Southern Blot, Plasmid Preparation, Agarose Gel Electrophoresis, Hybridization, Labeling, Marker, Real-time Polymerase Chain Reaction, Nucleic Acid Electrophoresis, Radioactivity

    Identification of the recombinant plasmid. (A) Detection of the housekeeping gene GAPDH. (B) Identification of the target gene by RT-PCR. (C) PCR product of the Fyn gene was subcloned into pMD18-T-Fyn and pEGFP-N1-Fyn. (D) Identification of pMD18-T-Fyn fragments produced by restriction enzyme digestion with Eco R I and Sma I. (E) Identification of pEGFP-N1-Fyn fragments generated by restriction enzyme digestion with Eco R I and Sma I. Lane 1, RT-PCR GAPDH product; Lane 2, negative control; Lane 3, RT-PCR Fyn product from brain; Lane 4, positive control; Lane 5, PCR product of pMD18-T-Fyn; Lane 6, PCR product of pEGFP-N1-Fyn; Lanes 7 and 9, positive control; Lanes 8 and 10, recombinant plasmid identification by digestion with EcoR I and Sma I (pMD18-T-Fyn, 2700 bp; pEGFP-N1, 4700 bp; Fyn, 1611 bp); Lane M, DNA marker.

    Journal: Journal of Veterinary Science

    Article Title: Mouse Fyn induces pseudopodium formation in Chinese hamster ovary cells

    doi: 10.4142/jvs.2014.15.1.111

    Figure Lengend Snippet: Identification of the recombinant plasmid. (A) Detection of the housekeeping gene GAPDH. (B) Identification of the target gene by RT-PCR. (C) PCR product of the Fyn gene was subcloned into pMD18-T-Fyn and pEGFP-N1-Fyn. (D) Identification of pMD18-T-Fyn fragments produced by restriction enzyme digestion with Eco R I and Sma I. (E) Identification of pEGFP-N1-Fyn fragments generated by restriction enzyme digestion with Eco R I and Sma I. Lane 1, RT-PCR GAPDH product; Lane 2, negative control; Lane 3, RT-PCR Fyn product from brain; Lane 4, positive control; Lane 5, PCR product of pMD18-T-Fyn; Lane 6, PCR product of pEGFP-N1-Fyn; Lanes 7 and 9, positive control; Lanes 8 and 10, recombinant plasmid identification by digestion with EcoR I and Sma I (pMD18-T-Fyn, 2700 bp; pEGFP-N1, 4700 bp; Fyn, 1611 bp); Lane M, DNA marker.

    Article Snippet: The PCR product was digested with EcoR I and Sma I, and ligated into a pEGFP-N1 vector (Clontech; Takara Bio) to produce the recombinant expression vector pEGFP-N1-Fyn.

    Techniques: Recombinant, Plasmid Preparation, Reverse Transcription Polymerase Chain Reaction, Polymerase Chain Reaction, Produced, Generated, Negative Control, Positive Control, Marker

    Circular map of cross-genus consensus chloroplast genome sequences of three Primulina species and Boea hygrometrica. The outermost circle is positions (in Kb) of consensus cp genome sequences. a Annotation of consensus cp genome sequences. Genes shown inside and outside of the circle are transcribed clockwise and counterclockwise, respectively, and their gene names are marked as black and red, respectively. Genes belonging to different groups are marked with different color, with the bar shown in the center. b Distribution of conserved regions of the four cp genomes. The grey columns represent the Con_Islands, which were defined as the regions containing over 50 continuous conserved sites in cross-genus consensus cp genome sequences. c Distribution of intrageneric and intergeneric polymorphism. The red and blue lines represent average intrageneric and intergeneric PICs in a 100 bp windows with a step of 25 bp, respectively. The PICs were counted by the sum of SNP and Indel between two cp genomes. d Distribution of restriction enzyme site of EcoRI . The outer to inner circles represent the distribution of EcoR I in P. eburnea, P. huaijiensis, P. linearifolia and B. hygrometrica in turns

    Journal: BMC Evolutionary Biology

    Article Title: The complete chloroplast genome of Primulina and two novel strategies for development of high polymorphic loci for population genetic and phylogenetic studies

    doi: 10.1186/s12862-017-1067-z

    Figure Lengend Snippet: Circular map of cross-genus consensus chloroplast genome sequences of three Primulina species and Boea hygrometrica. The outermost circle is positions (in Kb) of consensus cp genome sequences. a Annotation of consensus cp genome sequences. Genes shown inside and outside of the circle are transcribed clockwise and counterclockwise, respectively, and their gene names are marked as black and red, respectively. Genes belonging to different groups are marked with different color, with the bar shown in the center. b Distribution of conserved regions of the four cp genomes. The grey columns represent the Con_Islands, which were defined as the regions containing over 50 continuous conserved sites in cross-genus consensus cp genome sequences. c Distribution of intrageneric and intergeneric polymorphism. The red and blue lines represent average intrageneric and intergeneric PICs in a 100 bp windows with a step of 25 bp, respectively. The PICs were counted by the sum of SNP and Indel between two cp genomes. d Distribution of restriction enzyme site of EcoRI . The outer to inner circles represent the distribution of EcoR I in P. eburnea, P. huaijiensis, P. linearifolia and B. hygrometrica in turns

    Article Snippet: For RAD-Seq of the three populations, DNA was first treated with restriction enzyme EcoR I (Takara, China), and then several standard steps were performed, from the addition of sequencing adapters, interruption of enzyme digestion products, to break into smaller random pieces, and repairing the end based on existing protocols [ ].

    Techniques:

    Restriction enzyme digestion of the recombinant plasmid pET32a-CtCVNH by EcoR I and Hind III. M 1 : λ- H ind III digest DNA marker. 1, pET32a (+); 2, pET32a-CtCVNH; M 2 , 100 bp DNA ladder marker.

    Journal: International Journal of Molecular Sciences

    Article Title: Expression, Purification and Identification of CtCVNH, a Novel Anti-HIV (Human Immunodeficiency Virus) Protein from Ceratopteris thalictroides

    doi: 10.3390/ijms14047506

    Figure Lengend Snippet: Restriction enzyme digestion of the recombinant plasmid pET32a-CtCVNH by EcoR I and Hind III. M 1 : λ- H ind III digest DNA marker. 1, pET32a (+); 2, pET32a-CtCVNH; M 2 , 100 bp DNA ladder marker.

    Article Snippet: Experimental Materials Plasmid pET32a (+) and E. coli strain Rosetta 2 (DE3) were used as hosts for the expression of CVNH. λ-Hind III digest DNA marker, 100 bp DNA ladder marker, protein molecular weight marker (low) and restriction enzymes EcoR I and Hind III were supplied by TaKaRa (Dalian, China).

    Techniques: Recombinant, Plasmid Preparation, Marker

    Electrophoresis of PCR product and pUCm-T-ZNRD1 digested with enzymes. Lane 1: PCR product; Lane 2, 3: Marker (200 bp); Lane 4: recombinant of pUCm-T-ZNRD1 cleaved by EcoR V and Xba I; Lane 5: recombinant of pUCm-T-ZNRD1.

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells

    doi: 10.3748/wjg.v9.i5.894

    Figure Lengend Snippet: Electrophoresis of PCR product and pUCm-T-ZNRD1 digested with enzymes. Lane 1: PCR product; Lane 2, 3: Marker (200 bp); Lane 4: recombinant of pUCm-T-ZNRD1 cleaved by EcoR V and Xba I; Lane 5: recombinant of pUCm-T-ZNRD1.

    Article Snippet: EcoR V, Xba I, BamH I, cloning vector pUCm-T and T4 DNA ligase were purchased from Takara; MTT, DEPC from Sigma.

    Techniques: Electrophoresis, Polymerase Chain Reaction, Marker, Recombinant

    Electrophoresis identification of pcDNA3.1-anti ZNRD1. Lane 1: pcDNA3.1-anti ZNRD1/ EcoR V + BamH I; Lane 2: pcDNA3.1-anti ZNRD1; Lane 3: Marker (200 bp).

    Journal: World Journal of Gastroenterology : WJG

    Article Title: Effect of ZNRD1 gene antisense RNA on drug resistant gastric cancer cells

    doi: 10.3748/wjg.v9.i5.894

    Figure Lengend Snippet: Electrophoresis identification of pcDNA3.1-anti ZNRD1. Lane 1: pcDNA3.1-anti ZNRD1/ EcoR V + BamH I; Lane 2: pcDNA3.1-anti ZNRD1; Lane 3: Marker (200 bp).

    Article Snippet: EcoR V, Xba I, BamH I, cloning vector pUCm-T and T4 DNA ligase were purchased from Takara; MTT, DEPC from Sigma.

    Techniques: Electrophoresis, Marker

    Isolation of a segment of the gene np and construction of the expression vector. (A) Cloning representation of a gene coding for a segment of the protein NP from avian influenza virus subtype H7N1 comprising the aminoacids 49-375 (np 49-375 ) in the plasmid pUC-18 and in the expression vector pET-28a. (B) Isolation of the gene np 49-375 by PCR. 1- Molecular weight marker (MWM) (pAdEasy digested with the enzyme Apa I), 2- DNA segment corresponding to the gene np 49-375 , 3- PCR reaction with primers and without template. (C) Electrophoresis in agarose gel (1%) of the restriction analysis for the plasmid pUC-np 49-375 . 1- MWM, 2- Plasmid pUC-np 49-375 undigested, 3- Plasmid pUC18 digested with the enzymes Nhe I/EcoR I, 4- Plasmid pUC-np 49-375 digested with the enzymes Nhe I/EcoR I. (D) Electrophoresis in agarose gel (1%) of the restriction analysis for the plasmid pET-28a-np 49-375 . 1- MWM, 2- Plasmid pET-28a-np 49-375 undigested, 3- Plasmid pET-28a digested with the enzymes Nhe I/EcoR I, 4- Plasmid pET-28a-np 49-375 digested with the enzymes Nhe I/EcoR I.

    Journal: Open Veterinary Journal

    Article Title: Dual function of the hemagglutinin H5 fused to chicken CD154 in a potential strategy of DIVA against avian influenza disease: preliminary study

    doi:

    Figure Lengend Snippet: Isolation of a segment of the gene np and construction of the expression vector. (A) Cloning representation of a gene coding for a segment of the protein NP from avian influenza virus subtype H7N1 comprising the aminoacids 49-375 (np 49-375 ) in the plasmid pUC-18 and in the expression vector pET-28a. (B) Isolation of the gene np 49-375 by PCR. 1- Molecular weight marker (MWM) (pAdEasy digested with the enzyme Apa I), 2- DNA segment corresponding to the gene np 49-375 , 3- PCR reaction with primers and without template. (C) Electrophoresis in agarose gel (1%) of the restriction analysis for the plasmid pUC-np 49-375 . 1- MWM, 2- Plasmid pUC-np 49-375 undigested, 3- Plasmid pUC18 digested with the enzymes Nhe I/EcoR I, 4- Plasmid pUC-np 49-375 digested with the enzymes Nhe I/EcoR I. (D) Electrophoresis in agarose gel (1%) of the restriction analysis for the plasmid pET-28a-np 49-375 . 1- MWM, 2- Plasmid pET-28a-np 49-375 undigested, 3- Plasmid pET-28a digested with the enzymes Nhe I/EcoR I, 4- Plasmid pET-28a-np 49-375 digested with the enzymes Nhe I/EcoR I.

    Article Snippet: Subsequently, the DNA segment coding the protein NP49-375 was removed from the plasmid pUC-np49-375 by digestion with the enzymes Nhe I and EcoR I (Promega, USA) and inserted into the prokaryotic expression vector pET-28a (Invitrogen, USA), previously digested with the same enzymes, to obtain the final construction.

    Techniques: Isolation, Expressing, Plasmid Preparation, Clone Assay, Positron Emission Tomography, Polymerase Chain Reaction, Molecular Weight, Marker, Electrophoresis, Agarose Gel Electrophoresis

    Genetic mapping and molecular analysis of the opsX mutant strain. (A) The solid triangle indicates the site of the Tn5 transposon insertion. Restriction sites for EcoR I (E) are also indicated. (B, C) PCR and Southern hybridization analysis for confirmation of transposon insertion in opsX : M1, 1-kb ladder; M2, lambda DNA/ Hind III; lane 1, wild type; lanes 2 and 4, opsX mutant. The arrowhead indicates a single positive signal from the transposon (2,513-bp).

    Journal: The Plant Pathology Journal

    Article Title: DNA Microarray and Gene Ontology Enrichment Analysis Reveals That a Mutation in opsX Affects Virulence and Chemotaxis in Xanthomonas oryzae pv. oryzae

    doi: 10.5423/PPJ.OA.10.2015.0208

    Figure Lengend Snippet: Genetic mapping and molecular analysis of the opsX mutant strain. (A) The solid triangle indicates the site of the Tn5 transposon insertion. Restriction sites for EcoR I (E) are also indicated. (B, C) PCR and Southern hybridization analysis for confirmation of transposon insertion in opsX : M1, 1-kb ladder; M2, lambda DNA/ Hind III; lane 1, wild type; lanes 2 and 4, opsX mutant. The arrowhead indicates a single positive signal from the transposon (2,513-bp).

    Article Snippet: For Southern hybridization, genomic DNA was digested with EcoR I (Toyobo, Seoul, Korea), electrophoresed, and transferred to a Hybond-N+ membrane (GE Healthcare, Seoul, Korea).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Hybridization, Lambda DNA Preparation