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  • 99
    New England Biolabs single eco ri site ecori hf
    Single Eco Ri Site Ecori Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher eco ri site
    Eco Ri Site, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1324 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene eco ri site
    Eco Ri Site, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 841 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher eco ri restriction sites
    Eco Ri Restriction Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 306 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sangon Biotech eco ri restriction sites
    Eco Ri Restriction Sites, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 95/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Eco Ri Site, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 365 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega eco ri site
    Eco Ri Site, supplied by Promega, used in various techniques. Bioz Stars score: 92/100, based on 574 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa eco ri site
    Eco Ri Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 679 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenScript eco ri sites
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    Biomatik eco ri restriction sites
    Eco Ri Restriction Sites, supplied by Biomatik, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene eco ri restriction site
    Eco Ri Restriction Site, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenScript eco ri restriction sites
    Eco Ri Restriction Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 92/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Sage Science eco ri cut site
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    Glaxo Smith eco ri restriction sites
    Eco Ri Restriction Sites, supplied by Glaxo Smith, used in various techniques. Bioz Stars score: 81/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biomers.net eco ri restriction sites
    Eco Ri Restriction Sites, supplied by biomers.net, used in various techniques. Bioz Stars score: 90/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega restriction sites eco ri
    Restriction Sites Eco Ri, supplied by Promega, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher eco ri recognition site
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    Millipore eco ri restriction sites
    Eco Ri Restriction Sites, supplied by Millipore, used in various techniques. Bioz Stars score: 92/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore eco ri bamhi sites
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    Millipore eco ri bgl ii sites
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    Millipore hind iii eco ri site
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Hind Iii Eco Ri Site, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    79
    TaKaRa eco ri cloning site
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Eco Ri Cloning Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 79/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher bam hi eco ri sites
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Bam Hi Eco Ri Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 199 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GenScript eco ri cloning sites
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Eco Ri Cloning Sites, supplied by GenScript, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    TaKaRa eco ri restriction enzyme site
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Eco Ri Restriction Enzyme Site, supplied by TaKaRa, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher eco ri restriction enzyme sites
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Eco Ri Restriction Enzyme Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Midland Certified Reagent eco ri cloning sites
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
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    TaKaRa bgl ii eco ri sites underlined
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Bgl Ii Eco Ri Sites Underlined, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher eco ri n41890 restriction sites
    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
    Eco Ri N41890 Restriction Sites, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD <t>III)</t> were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, <t>SAP102,</t> and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.
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    Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD III) were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, SAP102, and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.

    Journal: The Journal of Neuroscience

    Article Title: Regulation of Dendritic Spine Morphogenesis by Insulin Receptor Substrate 53, a Downstream Effector of Rac1 and Cdc42 Small GTPases

    doi: 10.1523/JNEUROSCI.3212-04.2005

    Figure Lengend Snippet: Similar spatiotemporal expression patterns and complex formation of IRSp53 and PSD-95 in the brain. A ). Cb, Cerebellum; Ctx, cortex; Hc, hippocampus; St, striatum; R, the rest of the brain. B , Similar developmental expression patterns of IRSp53 and PSD-95. Membranes of rat brain at the indicated developmental stages were immunoblotted for IRSp53, PSD-95, and β-tubulin (control). E, Embryonic day; P, postnatal day; wk, week. C , Cofractionation of IRSp53 and PSD-95 in subcellular fractions of adult rat brain. Synaptophysin (SynPhy) was probed as a control. H, Homogenates; P2, crude synaptosomes; S2, supernatant after P2 precipitation; S3, cytosol; P3, light membranes; LP1, synaptosomal membranes; LS2, synaptosomal cytosol; LP2, synaptic vesicle-enriched fraction. D , Coenrichment of IRSp53 and PSD-95 in PSD fractions. PSD fractions of adult rat brain extracted with Triton X-100 once (PSD I), twice (PSD II), or with Triton X-100 and the strong detergent Sarcosyl (PSD III) were immunoblotted with IRSp53, PSD-95, and synaptophysin antibodies. E , DOC extracts of the crude synaptosomal fraction of adult rat brain were immunoprecipitated with IRSp53 antibodies or rabbit IgG (negative control) and immunoblotted with the indicated antibodies. Rb, Rabbit. F-J , The same extracts were immunoprecipitated with PSD-95, chapsyn-110, SAP97, SAP102, and S-SCAM antibodies or control IgGs and immunoblotted with the indicated antibodies. NR2B, NMDA glutamate receptor subunit 2 (positive control); Gp, guinea pig.

    Article Snippet: Mouse SAP102 (full length; aa 1-835) was subcloned into the Hind III- Eco RI site of the pFLAG-CMV2 vector (Sigma).

    Techniques: Expressing, Immunoprecipitation, Negative Control, Positive Control