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  • 75
    GE Healthcare ecl prime ge healthcare
    Ecl Prime Ge Healthcare, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 75/100, based on 476 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime ge healthcare/product/GE Healthcare
    Average 75 stars, based on 476 article reviews
    Price from $9.99 to $1999.99
    ecl prime ge healthcare - by Bioz Stars, 2020-02
    75/100 stars
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    80
    GE Healthcare ecl prime enhanced chemiluminescence kit
    Ecl Prime Enhanced Chemiluminescence Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 172 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime enhanced chemiluminescence kit/product/GE Healthcare
    Average 80 stars, based on 172 article reviews
    Price from $9.99 to $1999.99
    ecl prime enhanced chemiluminescence kit - by Bioz Stars, 2020-02
    80/100 stars
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    78
    GE Healthcare enhanced chemiluminescence prime reagents
    Enhanced Chemiluminescence Prime Reagents, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 76 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 78 stars, based on 76 article reviews
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    GE Healthcare ecl prime enhanced chemiluminescence system
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Enhanced Chemiluminescence System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 77 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    ecl prime enhanced chemiluminescence system - by Bioz Stars, 2020-02
    77/100 stars
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    78
    GE Healthcare enhanced chemiluminescence ecl prime western blotting detection reagent
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Enhanced Chemiluminescence Ecl Prime Western Blotting Detection Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enhanced chemiluminescence ecl prime western blotting detection reagent/product/GE Healthcare
    Average 78 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
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    82
    GE Healthcare horseradish peroxidase hrp enhanced chemiluminescence reagent
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Horseradish Peroxidase Hrp Enhanced Chemiluminescence Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 82/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 82 stars, based on 10 article reviews
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    99
    GE Healthcare ecl detection
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Detection, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 2561 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl detection/product/GE Healthcare
    Average 99 stars, based on 2561 article reviews
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    GE Healthcare ecl kit
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 16147 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl kit/product/GE Healthcare
    Average 99 stars, based on 16147 article reviews
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    GE Healthcare ecl reaction
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Reaction, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 279 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 279 article reviews
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    ecl reaction - by Bioz Stars, 2020-02
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    GE Healthcare ecl reagent
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 8420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl reagent/product/GE Healthcare
    Average 99 stars, based on 8420 article reviews
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    ecl reagent - by Bioz Stars, 2020-02
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    GE Healthcare amersham ecl prime
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Amersham Ecl Prime, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 642 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/amersham ecl prime/product/GE Healthcare
    Average 99 stars, based on 642 article reviews
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    amersham ecl prime - by Bioz Stars, 2020-02
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    GE Healthcare ecl prime reagent
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 646 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime reagent/product/GE Healthcare
    Average 99 stars, based on 646 article reviews
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    GE Healthcare ecl chemoluminescence revelation
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Chemoluminescence Revelation, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 77 stars, based on 2 article reviews
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    ecl chemoluminescence revelation - by Bioz Stars, 2020-02
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    80
    GE Healthcare ecl prime buffer
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Buffer, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 80/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 80 stars, based on 5 article reviews
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    81
    GE Healthcare ecl prime chemiluminescnece
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Chemiluminescnece, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 81/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime chemiluminescnece/product/GE Healthcare
    Average 81 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    ecl prime chemiluminescnece - by Bioz Stars, 2020-02
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    79
    GE Healthcare ecl prima reagens
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prima Reagens, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prima reagens/product/GE Healthcare
    Average 79 stars, based on 6 article reviews
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    GE Healthcare ecl prime chemistry
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Chemistry, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 77/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime chemistry/product/GE Healthcare
    Average 77 stars, based on 4 article reviews
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    ecl prime chemistry - by Bioz Stars, 2020-02
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    99
    GE Healthcare ecl detection system
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Detection System, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 591 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl detection system/product/GE Healthcare
    Average 99 stars, based on 591 article reviews
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    ecl detection system - by Bioz Stars, 2020-02
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    GE Healthcare ecl solution
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 1145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl solution/product/GE Healthcare
    Average 99 stars, based on 1145 article reviews
    Price from $9.99 to $1999.99
    ecl solution - by Bioz Stars, 2020-02
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    GE Healthcare ecl prime chemiluminescent substrate
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Chemiluminescent Substrate, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 93 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime chemiluminescent substrate/product/GE Healthcare
    Average 99 stars, based on 93 article reviews
    Price from $9.99 to $1999.99
    ecl prime chemiluminescent substrate - by Bioz Stars, 2020-02
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    GE Healthcare ecl prime blocking agent
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Blocking Agent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 320 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 320 article reviews
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    79
    GE Healthcare ecl prime chemiluminescent development
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Chemiluminescent Development, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 79 stars, based on 12 article reviews
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    92
    GE Healthcare chemiluminescence ecl prime
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Chemiluminescence Ecl Prime, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    chemiluminescence ecl prime - by Bioz Stars, 2020-02
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    99
    GE Healthcare ecl prime blocking reagent
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Blocking Reagent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 316 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 99 stars, based on 316 article reviews
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    ecl prime blocking reagent - by Bioz Stars, 2020-02
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    GE Healthcare ecl prime solution
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Prime Solution, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 99/100, based on 94 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl prime solution/product/GE Healthcare
    Average 99 stars, based on 94 article reviews
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    89
    GE Healthcare ecl primer blocking agent
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Ecl Primer Blocking Agent, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 89 stars, based on 19 article reviews
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    78
    GE Healthcare enhance chemiluminescense ecl prime
    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Enhance Chemiluminescense Ecl Prime, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 78/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
    Chemiluminescent Ecl Prime Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
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    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
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    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
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    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system <t>(ECL,</t> <t>Amersham</t> Biosciences) and visualized using a G:BOX Chemi Syngene System.
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    Image Search Results


    PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized using a G:BOX Chemi Syngene System.

    Journal: PLoS Pathogens

    Article Title: Synthetic prions with novel strain-specified properties

    doi: 10.1371/journal.ppat.1005354

    Figure Lengend Snippet: PMCA analysis of raw fibrils and cell lysates from infected N2a and GT1 cell lines. Seeding ability of amyloid #4 and #28 by means of PMCA using brain homogenates of CD1 mice as substrates for amplification (A). PMCA analysis of GT1 and N2a cell lysates (infected with preparations #4 and #28) and collected at passage six (P6) after the infection (B). Black arrow indicates PK-resistant PrP. Asterisk indicates unspecific binding. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized using a G:BOX Chemi Syngene System.

    Article Snippet: Blots were developed using the ECL Prime detection system (Amersham) and visualized using a G:BOX Chemi Syngene system.

    Techniques: Infection, Mouse Assay, Amplification, Binding Assay, Western Blot

    Western blot analysis of amyloid fibrils performed under reducing and non-reducing SDS-PAGE. Monomeric (indicated by blue arrow) recMoPrP(23–231) was converted into amyloid forms by intermolecular disulfide linkage following the REDOX process. Western blotting of non-reducing Sodium Dodecyl Sulphate—PolyAcrylamide Gel Electrophoresis (SDS-PAGE) showing the conversion of recMoPrP(23–231) to amyloids is indicated by dimer (green arrow), trimer (red arrow), and more complex structures in both processes (A). Western blotting of reducing SDS-PAGE after treatment of amyloid with Dithiothreitol reducing agent (DTT) shows the decrease in signals of dimer, trimer and more complicated structures in all lanes of amyloid samples from REDOX-process (B). Western blotting of reducing SDS-PAGE of amyloid after a 3-day treatment with denaturant (6M Gdn-HCl), and subsequently with reducing agent DTT shows only monomeric recMoPrP(23–231) bands and the disappearance of more complicated structures in all lanes of amyloid samples in REDOX process (C). Western blots were performed using Fab D18 monoclonal antibody (1μg/mL). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences)

    Journal: PLoS Pathogens

    Article Title: Synthetic prions with novel strain-specified properties

    doi: 10.1371/journal.ppat.1005354

    Figure Lengend Snippet: Western blot analysis of amyloid fibrils performed under reducing and non-reducing SDS-PAGE. Monomeric (indicated by blue arrow) recMoPrP(23–231) was converted into amyloid forms by intermolecular disulfide linkage following the REDOX process. Western blotting of non-reducing Sodium Dodecyl Sulphate—PolyAcrylamide Gel Electrophoresis (SDS-PAGE) showing the conversion of recMoPrP(23–231) to amyloids is indicated by dimer (green arrow), trimer (red arrow), and more complex structures in both processes (A). Western blotting of reducing SDS-PAGE after treatment of amyloid with Dithiothreitol reducing agent (DTT) shows the decrease in signals of dimer, trimer and more complicated structures in all lanes of amyloid samples from REDOX-process (B). Western blotting of reducing SDS-PAGE of amyloid after a 3-day treatment with denaturant (6M Gdn-HCl), and subsequently with reducing agent DTT shows only monomeric recMoPrP(23–231) bands and the disappearance of more complicated structures in all lanes of amyloid samples in REDOX process (C). Western blots were performed using Fab D18 monoclonal antibody (1μg/mL). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences)

    Article Snippet: Blots were developed using the ECL Prime detection system (Amersham) and visualized using a G:BOX Chemi Syngene system.

    Techniques: Western Blot, SDS Page, Polyacrylamide Gel Electrophoresis

    PK digestion assay of amyloid #4, #19, #28 and #32. Western blotting of PK digestion assay (amyloids #4, #19, #28, #32) showed partial protease K (PK) resistance of recMoPrP(23–231) (amyloids #4 and #28). RecMoPrP(23–231) amyloids (PK- lanes) were digested with PK at ratio 1:10 (w/w) (PK+ lanes) and 1:1 (w/w) (PK++ lanes). Western blots were performed using Fab D18 monoclonal antibody (1μg/mL). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences)

    Journal: PLoS Pathogens

    Article Title: Synthetic prions with novel strain-specified properties

    doi: 10.1371/journal.ppat.1005354

    Figure Lengend Snippet: PK digestion assay of amyloid #4, #19, #28 and #32. Western blotting of PK digestion assay (amyloids #4, #19, #28, #32) showed partial protease K (PK) resistance of recMoPrP(23–231) (amyloids #4 and #28). RecMoPrP(23–231) amyloids (PK- lanes) were digested with PK at ratio 1:10 (w/w) (PK+ lanes) and 1:1 (w/w) (PK++ lanes). Western blots were performed using Fab D18 monoclonal antibody (1μg/mL). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences)

    Article Snippet: Blots were developed using the ECL Prime detection system (Amersham) and visualized using a G:BOX Chemi Syngene system.

    Techniques: Western Blot

    Detection of PMCA-#4 in brain and blood of infected animals and related immunohistochemical/biochemical characterization. Neuropathological analysis of PMCA-#4 and PMCA-PBS injected animals and comparison to that of RML infected mice. Animals injected with PMCA-#4 showed widespread deposition of PrP Res in the hippocampus with focal plaque-like deposits found in the cerebral cortex. Cerebellum is completely spared by PrP Res accumulation. RML injected animals showed the typical pattern of widespread, synaptic and diffuse PrP Res accumulation in the whole brain with major involvement of thalamus and hippocampus. Spongiform changes were mainly found in the hippocampus of PMCA-#4 injected animals. Few vacuoles were detected in the cerebral cortex, while cerebellum did not show any vacuolation. RML injected animals showed severe vacuolation in the thalamus and hippocampus. Mild alterations were found in cerebellum and septum. Brain of PMCA-PBS injected animal was used as control (A). Higher magnification of the red square in panel A (see asterisk) and Thioflavin-S staining showing the lack of amyloid properties of the deposits found in the submeningeal level of the cerebral cortex (B). PMCA of blood collected at 140 day post infection (d.p.i.) from a symptomatic animal injected with PMCA-#4. RML dilutions (10 −10 and 10 −11 ) were used in PMCA to estimate the concentration of circulating infectious PrP (C). Biochemical analysis of the brains harvested from the first two animals injected with PMCA-#4 (sacrificed at terminal stage of the disease) were performed and compared to that of RML injected mice (D). Scale bar in A is 10 μm; scale bar in B is 5 μm. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized using a G:BOX Chemi Syngene system.

    Journal: PLoS Pathogens

    Article Title: Synthetic prions with novel strain-specified properties

    doi: 10.1371/journal.ppat.1005354

    Figure Lengend Snippet: Detection of PMCA-#4 in brain and blood of infected animals and related immunohistochemical/biochemical characterization. Neuropathological analysis of PMCA-#4 and PMCA-PBS injected animals and comparison to that of RML infected mice. Animals injected with PMCA-#4 showed widespread deposition of PrP Res in the hippocampus with focal plaque-like deposits found in the cerebral cortex. Cerebellum is completely spared by PrP Res accumulation. RML injected animals showed the typical pattern of widespread, synaptic and diffuse PrP Res accumulation in the whole brain with major involvement of thalamus and hippocampus. Spongiform changes were mainly found in the hippocampus of PMCA-#4 injected animals. Few vacuoles were detected in the cerebral cortex, while cerebellum did not show any vacuolation. RML injected animals showed severe vacuolation in the thalamus and hippocampus. Mild alterations were found in cerebellum and septum. Brain of PMCA-PBS injected animal was used as control (A). Higher magnification of the red square in panel A (see asterisk) and Thioflavin-S staining showing the lack of amyloid properties of the deposits found in the submeningeal level of the cerebral cortex (B). PMCA of blood collected at 140 day post infection (d.p.i.) from a symptomatic animal injected with PMCA-#4. RML dilutions (10 −10 and 10 −11 ) were used in PMCA to estimate the concentration of circulating infectious PrP (C). Biochemical analysis of the brains harvested from the first two animals injected with PMCA-#4 (sacrificed at terminal stage of the disease) were performed and compared to that of RML injected mice (D). Scale bar in A is 10 μm; scale bar in B is 5 μm. Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized using a G:BOX Chemi Syngene system.

    Article Snippet: Blots were developed using the ECL Prime detection system (Amersham) and visualized using a G:BOX Chemi Syngene system.

    Techniques: Infection, Immunohistochemistry, Injection, Mouse Assay, Staining, Concentration Assay, Western Blot

    PK digestion assay of GT1 and N2a cells collected at different passages after infection with amyloid fibrils. Western blotting of GT1 and N2a cell lines infected with PrP amyloid #4 was observed throughout, from first passage (P1) to sixth passage (P6) and after treatment with proteinase K at ratio 1:500 (w/w). Western blot was performed using Fab D18 monoclonal antibody (1μg/mL). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences).

    Journal: PLoS Pathogens

    Article Title: Synthetic prions with novel strain-specified properties

    doi: 10.1371/journal.ppat.1005354

    Figure Lengend Snippet: PK digestion assay of GT1 and N2a cells collected at different passages after infection with amyloid fibrils. Western blotting of GT1 and N2a cell lines infected with PrP amyloid #4 was observed throughout, from first passage (P1) to sixth passage (P6) and after treatment with proteinase K at ratio 1:500 (w/w). Western blot was performed using Fab D18 monoclonal antibody (1μg/mL). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences).

    Article Snippet: Blots were developed using the ECL Prime detection system (Amersham) and visualized using a G:BOX Chemi Syngene system.

    Techniques: Infection, Western Blot

    Seeding assay of N2a and GT1 cell cultures with synthetic amyloid fibrils. Seeding of recMoPrP(23–231) amyloid preparations induced the conversion of endogenous PrP C to mildly PK resistant forms (A) and accumulation (B) in mouse neuroblastoma N2a and mouse hypothalamic GT1 amyloid-infected cell lines analyzed six passages after the infection (P6). Western blotting shows the partial protease K (PK) resistance of N2a and GT1 amyloid fibril-infected cell lysates. Fibril-infected cell lysates (PK-lanes) were digested with PK at ratio 1:500 (w/w) (PK+ lanes). Western blots were performed using Fab D18 monoclonal antibody (1μg/mL) for GT1 infected cells and Clone-P (1μg/mL) for N2a infected cells. Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences) (A). Immunofluorescence imaging shows the accumulations of PrP in N2a and GT1 amyloid fibril-infected cell lines. The deposition and level of PrP (green) in amyloid fibril-infected cell lines after six passages were detected by Fab D18 monoclonal antibody (10 μg/mL final concentration). The nuclei (blue) were stained with DAPI. Scale bar is 20μm (B).

    Journal: PLoS Pathogens

    Article Title: Synthetic prions with novel strain-specified properties

    doi: 10.1371/journal.ppat.1005354

    Figure Lengend Snippet: Seeding assay of N2a and GT1 cell cultures with synthetic amyloid fibrils. Seeding of recMoPrP(23–231) amyloid preparations induced the conversion of endogenous PrP C to mildly PK resistant forms (A) and accumulation (B) in mouse neuroblastoma N2a and mouse hypothalamic GT1 amyloid-infected cell lines analyzed six passages after the infection (P6). Western blotting shows the partial protease K (PK) resistance of N2a and GT1 amyloid fibril-infected cell lysates. Fibril-infected cell lysates (PK-lanes) were digested with PK at ratio 1:500 (w/w) (PK+ lanes). Western blots were performed using Fab D18 monoclonal antibody (1μg/mL) for GT1 infected cells and Clone-P (1μg/mL) for N2a infected cells. Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized on Hyperfilm (Amersham Biosciences) (A). Immunofluorescence imaging shows the accumulations of PrP in N2a and GT1 amyloid fibril-infected cell lines. The deposition and level of PrP (green) in amyloid fibril-infected cell lines after six passages were detected by Fab D18 monoclonal antibody (10 μg/mL final concentration). The nuclei (blue) were stained with DAPI. Scale bar is 20μm (B).

    Article Snippet: Blots were developed using the ECL Prime detection system (Amersham) and visualized using a G:BOX Chemi Syngene system.

    Techniques: Infection, Western Blot, Immunofluorescence, Imaging, Concentration Assay, Staining

    Amplification and characterization of a new prion isolate obtained from the brain of amyloid-infected animals. PMCA assessment using the brain homogenate of injected animals (with amyloid #4 or #28) as seed and the brain of wild type CD1 animals as substrate. Serial dilution of RML prion strain were used as internal control for PMCA efficiency (A). Western blot (B) and PNGase comparison (C) of amplified clone #4 (PMCA-#4) with known prion strains (RML, mouse adapted vCJD and ME7). Read arrows in B and C indicate the different electrophoretic mobility of the unglycosylated PrP band of PMCA-#4 (migrating at around 20kDa) compared to that of known prion strains migrating at 19 kDa or 21 kDa (black arrows). Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized using a G:BOX Chemi Syngene system.

    Journal: PLoS Pathogens

    Article Title: Synthetic prions with novel strain-specified properties

    doi: 10.1371/journal.ppat.1005354

    Figure Lengend Snippet: Amplification and characterization of a new prion isolate obtained from the brain of amyloid-infected animals. PMCA assessment using the brain homogenate of injected animals (with amyloid #4 or #28) as seed and the brain of wild type CD1 animals as substrate. Serial dilution of RML prion strain were used as internal control for PMCA efficiency (A). Western blot (B) and PNGase comparison (C) of amplified clone #4 (PMCA-#4) with known prion strains (RML, mouse adapted vCJD and ME7). Read arrows in B and C indicate the different electrophoretic mobility of the unglycosylated PrP band of PMCA-#4 (migrating at around 20kDa) compared to that of known prion strains migrating at 19 kDa or 21 kDa (black arrows). Western blots were performed using 6D11 monoclonal antibody to PrP (0.2 μg/mL, Covance). Blots were developed with the enhanced chemiluminescent system (ECL, Amersham Biosciences) and visualized using a G:BOX Chemi Syngene system.

    Article Snippet: Blots were developed using the ECL Prime detection system (Amersham) and visualized using a G:BOX Chemi Syngene system.

    Techniques: Amplification, Infection, Injection, Serial Dilution, Western Blot