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  • 90
    Thermo Fisher standard enhanced chemiluminescence ecl detection kit
    Standard Enhanced Chemiluminescence Ecl Detection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore ecl detection kit
    Ecl Detection Kit, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 846 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare ecl enhanced chemiluminescence detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Ecl Enhanced Chemiluminescence Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1356 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher enhanced chemiluminescence ecl detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Enhanced Chemiluminescence Ecl Detection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare ecl enhanced chemiluminescent detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Ecl Enhanced Chemiluminescent Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bio-Rad ecl enhanced chemiluminescence detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Ecl Enhanced Chemiluminescence Detection Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio ecl reagent
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Ecl Reagent, supplied by Boster Bio, used in various techniques. Bioz Stars score: 93/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biological Industries Inc ez enhanced chemiluminescence ecl detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Ez Enhanced Chemiluminescence Ecl Detection Kit, supplied by Biological Industries Inc, used in various techniques. Bioz Stars score: 85/100, based on 28 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare super enhanced chemiluminescence ecl detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Super Enhanced Chemiluminescence Ecl Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Advansta westernbright enhanced chemiluminescence ecl detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Westernbright Enhanced Chemiluminescence Ecl Detection Kit, supplied by Advansta, used in various techniques. Bioz Stars score: 91/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Boster Bio enhanced chemiluminescent ecl detection kit rabbit igg
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Enhanced Chemiluminescent Ecl Detection Kit Rabbit Igg, supplied by Boster Bio, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare ecl enhanced chemiluminescence western blotting detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Ecl Enhanced Chemiluminescence Western Blotting Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vazyme Biotech Co high sensitivity enhanced chemiluminescence ecl detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    High Sensitivity Enhanced Chemiluminescence Ecl Detection Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Fisher Scientific enhanced chemiluminescence 2 detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Enhanced Chemiluminescence 2 Detection Kit, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher supersignal ecl detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Supersignal Ecl Detection Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare hrp substrate enhanced chemiluminescence ecl detection kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Hrp Substrate Enhanced Chemiluminescence Ecl Detection Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    GE Healthcare enhanced chemiluminiscence ecl kit
    Top , Western blot analysis of human <t>TRX</t> expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an <t>ECL</t> detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.
    Enhanced Chemiluminiscence Ecl Kit, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Top , Western blot analysis of human TRX expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an ECL detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.

    Journal: The Journal of Experimental Medicine

    Article Title: Pancreatic ? Cell-specific Expression of Thioredoxin, an Antioxidative and Antiapoptotic Protein, Prevents Autoimmune and Streptozotocin-induced Diabetes

    doi:

    Figure Lengend Snippet: Top , Western blot analysis of human TRX expression in pancreatic islets. Pancreatic islets were isolated from 8-wk-old (NOD × B6) F1 mice and their nontransgenic littermates. Approximately 15 μg of islet protein was loaded in each well. The amounts of human TRX in the lysates of transgenic islets from lines 90 and 21 mice were densitometrically estimated to be 25 ng on the imaging film exposed using an ECL detection kit. Prolonged exposure of a film revealed a faint band of human TRX in line 48 (data not shown). Bottom , Immunoblot analysis of the endogenous TRX expression in islets and pancreas. The lysates of islets were prepared from (NOD × B6) F1 transgenic mice (line 90), their TRX-negative littermates, and BALB/c mice. The lysates of the pancreas were prepared from a (NOD × B6) F1 transgenic mouse. These lysates were subjected to Western blot analysis using mouse TRX–specific serum. 2.5 μg ( a ) and 5 μg ( b ) of protein were loaded for each sample. The imaging film was exposed overnight.

    Article Snippet: Human and murine TRX were visualized using an ECL detection kit ( Amersham Pharmacia Biotech ).

    Techniques: Western Blot, Expressing, Isolation, Mouse Assay, Transgenic Assay, Imaging