ecl anti-rabbit igg Search Results


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  • 93
    Millipore ecl anti rabbit igg
    Ecl Anti Rabbit Igg, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl anti rabbit igg/product/Millipore
    Average 93 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
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    89
    GE Healthcare ecl anti rabbit igg
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Ecl Anti Rabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 89/100, based on 391 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ecl anti rabbit igg/product/GE Healthcare
    Average 89 stars, based on 391 article reviews
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    89/100 stars
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    85
    GE Healthcare ecl anti rabbit igg na9340v
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Ecl Anti Rabbit Igg Na9340v, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 5 article reviews
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    85
    GE Healthcare anti rabbit igg ecl antidody
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Anti Rabbit Igg Ecl Antidody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare ecl donkey anti rabbit igg
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Ecl Donkey Anti Rabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 62 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare ecl anti rabbit hrp linked igg
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Ecl Anti Rabbit Hrp Linked Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 85 stars, based on 15 article reviews
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    86
    GE Healthcare ecl anti rabbit igg horseradish peroxidase
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Ecl Anti Rabbit Igg Horseradish Peroxidase, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    Santa Cruz Biotechnology igg ecl anti rabbit
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Igg Ecl Anti Rabbit, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    GE Healthcare ecl plex anti rabbit igg cy5
    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the <t>ECL</t> anti-rabbit <t>IgG,</t> horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).
    Ecl Plex Anti Rabbit Igg Cy5, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare hrp conjugated ecl anti rabbit igg
    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit <t>IgG</t> (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding <t>HRP-conjugated</t> secondary anti-rabbit and anti-goat IgG antibodies were used together with <t>ECL</t> reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .
    Hrp Conjugated Ecl Anti Rabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    GE Healthcare peroxidase linked ecl anti rabbit igg
    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit <t>IgG</t> (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding <t>HRP-conjugated</t> secondary anti-rabbit and anti-goat IgG antibodies were used together with <t>ECL</t> reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .
    Peroxidase Linked Ecl Anti Rabbit Igg, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Santa Cruz Biotechnology ecl hrp linked rabbit igg
    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit <t>IgG</t> (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding <t>HRP-conjugated</t> secondary anti-rabbit and anti-goat IgG antibodies were used together with <t>ECL</t> reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .
    Ecl Hrp Linked Rabbit Igg, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare ecl peroxidase labeled anti rabbit igg antibody
    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit <t>IgG</t> (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding <t>HRP-conjugated</t> secondary anti-rabbit and anti-goat IgG antibodies were used together with <t>ECL</t> reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .
    Ecl Peroxidase Labeled Anti Rabbit Igg Antibody, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    GE Healthcare ecl donkey anti rabbit igg hrp
    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit <t>IgG</t> (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding <t>HRP-conjugated</t> secondary anti-rabbit and anti-goat IgG antibodies were used together with <t>ECL</t> reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .
    Ecl Donkey Anti Rabbit Igg Hrp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    GE Healthcare ecl anti rabbit igg hrp na9340v
    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit <t>IgG</t> (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding <t>HRP-conjugated</t> secondary anti-rabbit and anti-goat IgG antibodies were used together with <t>ECL</t> reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .
    Ecl Anti Rabbit Igg Hrp Na9340v, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Avantor ecl donkey anti rabbit igg hrp
    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit <t>IgG</t> (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding <t>HRP-conjugated</t> secondary anti-rabbit and anti-goat IgG antibodies were used together with <t>ECL</t> reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .
    Ecl Donkey Anti Rabbit Igg Hrp, supplied by Avantor, used in various techniques. Bioz Stars score: 88/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the ECL anti-rabbit IgG, horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).

    Journal: The Journal of Biological Chemistry

    Article Title: Heat Shock Protein gp96 Interacts with Protein Phosphatase 5 and Controls Toll-like Receptor 2 (TLR2)-mediated Activation of Extracellular Signal-regulated Kinase (ERK) 1/2 in Post-hypoxic Kidney Cells *Heat Shock Protein gp96 Interacts with Protein Phosphatase 5 and Controls Toll-like Receptor 2 (TLR2)-mediated Activation of Extracellular Signal-regulated Kinase (ERK) 1/2 in Post-hypoxic Kidney Cells * S⃞

    doi: 10.1074/jbc.M808376200

    Figure Lengend Snippet: PP5 interacts with gp96. Lysates from non-hypoxic PKSV-PR cells ( A ) and wild-type or Tlr2 -/- RTECs ( B, C ) were subjected to IP using an antibody against gp96. The IP material was then subjected to Western blot analysis, and proteins were detected with anti-PP5 or anti-gp96 antibodies, then revealed with the ECL anti-rabbit IgG, horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) ( A, lanes 1-4 and C ) or the Rabbit IgG TrueBlot™ (eBioscience) ( A, lanes 5 and 6, and B ). A, untreated PKSV-PR cells lysates were immunoprecipitated (IP) with the anti-gp96 antibody, then blotted for PP5 ( lane 1 ). As controls, immunoprecipitated cell lysates were blotted for gp96 ( lane 2 ) and non-immunoprecipitated PKSV-PR cell lysates ( None ) were blotted for PP5 ( lane 3 ) or gp96 ( lane 4 ). Western blot identification of PP5 in non-hypoxic PKSV-PR cell lysates from co-IP ( lane 5 ) and non-immunoprecipitated ( None, lane 6 ) cell lysates using the secondary IgG TrueBlot™ antibody. B and C , untreated control ( C ) and post-hypoxic ( H ) WT or Tlr2 -/- RTECs were immunoprecipitated with the anti-gp96 antibody, then blotted for PP5 ( B ) or gp96 ( C ). As controls, non-immunoprecipitated cell lysates were blotted either for PP5 ( B ) or gp96 ( C ).

    Article Snippet: After being transferred onto nitrocellulose, the filters were incubated with antibodies directed against gp96 (1:2000), or PP5 (1:1000), and then with the ECL anti-rabbit IgG, horseradish peroxidase-linked species-specific whole antibody (GE Healthcare) or the Rabbit IgG TrueBlot™ (eBioscience).

    Techniques: Western Blot, Immunoprecipitation, Co-Immunoprecipitation Assay

    Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit IgG (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding HRP-conjugated secondary anti-rabbit and anti-goat IgG antibodies were used together with ECL reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .

    Journal: Scientific Reports

    Article Title: Epitopes of anti-RIFIN antibodies and characterization of rif-expressing Plasmodium falciparum parasites by RNA sequencing

    doi: 10.1038/srep43190

    Figure Lengend Snippet: Western blots of RIFINs in multiple parasite strains. SDS-extracted parasite lysates of S1.2R, S1.2NR, FCR3CSA, NF54CSA, 3D7CD36ICAM1 and IT4CD36ICAM1 were run on SDS-PAGE (lanes 1-6 respectively), transferred to nitrocellulose membrane and blotted with rabbit anti-PfHsp70 (1:2000), non-immune rabbit IgG (10 μg/ml), RαRIF C (rabbit anti-A-RIFIN C-terminus) IgG (10 μg/ml), RαRIF I (rabbit anti-A-RIFIN indel) IgG (10 μg/ml), non-immune goat IgG (10 μg/ml) and GαRIF (goat anti-A-RIFIN full length) IgG (10 μg/ml). Corresponding HRP-conjugated secondary anti-rabbit and anti-goat IgG antibodies were used together with ECL reagent for detection. Description of the antigens used for immunizations of RαRIF C , RαRIF I and GαRIF are described in Supplementary Table S1 and diagrammatically illustrated in Fig. 3D .

    Article Snippet: After 1 hr incubation at RT, membranes were washed three times in TBST and incubated with the corresponding HRP-conjugated ECL anti-rabbit IgG (GE Healthcare) or anti-goat IgG (Life Technologies) antibodies (both 1:5000 dilution) for a further 1 hr.

    Techniques: Western Blot, SDS Page