eb2 fraction Search Results


eb2  (Absea Inc)
86
Absea Inc eb2
(A) NHDFs or SK-N-SHs treated with non-targeting (ctrl), EB1, or CLIP-170 (CLIP) siRNAs were mock-infected or infected with HSV-1 at MOI 10 for 5 h and analyzed by WB. (B) SK-N-SHs treated with the indicated siRNAs were infected, as in (A). (C) SK-N-SHs treated with independent <t>EB2</t> siRNAs (I or II) were infected, as in (A). (D) SK-N-SHs were treated with 100 nM BafA or DMSO and infected at MOI 10 with VSV for 4 h or HSV-1 for 5 h. (E) NHDFs or SK-N-SHs analyzed by WB using the indicated antibodies. (F) NHDFs or SK-N-SHs stained for tyrosinated (Tyr), detyrosinated (Detyr), and acetylated (Ac) tubulin. Nuclei were stained with Hoechst. (G) NHDFs or SK-N-SHs treated with 500 nM DMSO or 10 μM nocodazole were infected at MOI 20 with HSV-1 for 4 h in the presence of 1 μg/mL actinomycin D. Fixed cells were stained for VP5 and with Hoechst. Assessed for the accumulation of VP5 over 2 biological replicates were ≥ 165 NHDF or ≥ 190 SK-N-SH nuclei; error bars, SEMs; *p < 0.05, **p < 0.01, N.S., not significant; unpaired 2-tailed t test. (H) Cells treated as in (G) were infected at MOI 10 with HSV-1 for 5 h. All of the experiments represent ≥3 replicates unless indicated. See also and .
Eb2, supplied by Absea Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eb2/product/Absea Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
eb2 - by Bioz Stars, 2025-01
86/100 stars
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eb2 extrabond  (Scharlau Group)
86
Scharlau Group eb2 extrabond
(A) NHDFs or SK-N-SHs treated with non-targeting (ctrl), EB1, or CLIP-170 (CLIP) siRNAs were mock-infected or infected with HSV-1 at MOI 10 for 5 h and analyzed by WB. (B) SK-N-SHs treated with the indicated siRNAs were infected, as in (A). (C) SK-N-SHs treated with independent <t>EB2</t> siRNAs (I or II) were infected, as in (A). (D) SK-N-SHs were treated with 100 nM BafA or DMSO and infected at MOI 10 with VSV for 4 h or HSV-1 for 5 h. (E) NHDFs or SK-N-SHs analyzed by WB using the indicated antibodies. (F) NHDFs or SK-N-SHs stained for tyrosinated (Tyr), detyrosinated (Detyr), and acetylated (Ac) tubulin. Nuclei were stained with Hoechst. (G) NHDFs or SK-N-SHs treated with 500 nM DMSO or 10 μM nocodazole were infected at MOI 20 with HSV-1 for 4 h in the presence of 1 μg/mL actinomycin D. Fixed cells were stained for VP5 and with Hoechst. Assessed for the accumulation of VP5 over 2 biological replicates were ≥ 165 NHDF or ≥ 190 SK-N-SH nuclei; error bars, SEMs; *p < 0.05, **p < 0.01, N.S., not significant; unpaired 2-tailed t test. (H) Cells treated as in (G) were infected at MOI 10 with HSV-1 for 5 h. All of the experiments represent ≥3 replicates unless indicated. See also and .
Eb2 Extrabond, supplied by Scharlau Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eb2 extrabond/product/Scharlau Group
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
eb2 extrabond - by Bioz Stars, 2025-01
86/100 stars
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eb2 cartridges  (Scharlau Group)
86
Scharlau Group eb2 cartridges
(A) NHDFs or SK-N-SHs treated with non-targeting (ctrl), EB1, or CLIP-170 (CLIP) siRNAs were mock-infected or infected with HSV-1 at MOI 10 for 5 h and analyzed by WB. (B) SK-N-SHs treated with the indicated siRNAs were infected, as in (A). (C) SK-N-SHs treated with independent <t>EB2</t> siRNAs (I or II) were infected, as in (A). (D) SK-N-SHs were treated with 100 nM BafA or DMSO and infected at MOI 10 with VSV for 4 h or HSV-1 for 5 h. (E) NHDFs or SK-N-SHs analyzed by WB using the indicated antibodies. (F) NHDFs or SK-N-SHs stained for tyrosinated (Tyr), detyrosinated (Detyr), and acetylated (Ac) tubulin. Nuclei were stained with Hoechst. (G) NHDFs or SK-N-SHs treated with 500 nM DMSO or 10 μM nocodazole were infected at MOI 20 with HSV-1 for 4 h in the presence of 1 μg/mL actinomycin D. Fixed cells were stained for VP5 and with Hoechst. Assessed for the accumulation of VP5 over 2 biological replicates were ≥ 165 NHDF or ≥ 190 SK-N-SH nuclei; error bars, SEMs; *p < 0.05, **p < 0.01, N.S., not significant; unpaired 2-tailed t test. (H) Cells treated as in (G) were infected at MOI 10 with HSV-1 for 5 h. All of the experiments represent ≥3 replicates unless indicated. See also and .
Eb2 Cartridges, supplied by Scharlau Group, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/eb2 cartridges/product/Scharlau Group
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
eb2 cartridges - by Bioz Stars, 2025-01
86/100 stars
  Buy from Supplier
total eb2  (Abcam)
86
Abcam total eb2
(A) NHDFs or SK-N-SHs treated with non-targeting (ctrl), EB1, or CLIP-170 (CLIP) siRNAs were mock-infected or infected with HSV-1 at MOI 10 for 5 h and analyzed by WB. (B) SK-N-SHs treated with the indicated siRNAs were infected, as in (A). (C) SK-N-SHs treated with independent <t>EB2</t> siRNAs (I or II) were infected, as in (A). (D) SK-N-SHs were treated with 100 nM BafA or DMSO and infected at MOI 10 with VSV for 4 h or HSV-1 for 5 h. (E) NHDFs or SK-N-SHs analyzed by WB using the indicated antibodies. (F) NHDFs or SK-N-SHs stained for tyrosinated (Tyr), detyrosinated (Detyr), and acetylated (Ac) tubulin. Nuclei were stained with Hoechst. (G) NHDFs or SK-N-SHs treated with 500 nM DMSO or 10 μM nocodazole were infected at MOI 20 with HSV-1 for 4 h in the presence of 1 μg/mL actinomycin D. Fixed cells were stained for VP5 and with Hoechst. Assessed for the accumulation of VP5 over 2 biological replicates were ≥ 165 NHDF or ≥ 190 SK-N-SH nuclei; error bars, SEMs; *p < 0.05, **p < 0.01, N.S., not significant; unpaired 2-tailed t test. (H) Cells treated as in (G) were infected at MOI 10 with HSV-1 for 5 h. All of the experiments represent ≥3 replicates unless indicated. See also and .
Total Eb2, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total eb2/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
total eb2 - by Bioz Stars, 2025-01
86/100 stars
  Buy from Supplier
p eb2 s222  (Covalab Inc)
86
Covalab Inc p eb2 s222
Induced Neurons Model Molecular Consequences of CDKL5 Variants but Display NoTranscriptional, Morphological or Functional Changes. A) Normalized log fold gene expression for iN samples. B) Representative western blots for <t>EB2</t> and phosphorylation of EB2 S221 with TUJ1/Beta-3-tubulin as a loading control. C) Paired t-test of phosphorylation of EB2 fold change relative to EB2 between isogenic control and CDD patient-derived neurons (N=3 for 2 donors). D) Pearson correlation coefficients for gene expression for the 5000 most variable genes between all samples from iNs. E) Volcano plot of differentially expressed genes between pooled isogenic control and CDD patient-derived samples (N=6 per genotype). F) Representative images of iN neurites immunolabeled with TUJ1 and MAP2 in both patient and isogenic control lines. A paired t-test was performed for changes in neurite length normalized to the cell body area (μm/μm 2 ) for G) MAP2 immunofluorescence (N=3 batches). H) Synchrony index and I) weighted mean firing rate for INs during the first 30 days of maturation post-plating (N=4 differentiation batches, 2 donors). Error bars represent the standard error of the mean.
P Eb2 S222, supplied by Covalab Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/p eb2 s222/product/Covalab Inc
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
p eb2 s222 - by Bioz Stars, 2025-01
86/100 stars
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Image Search Results


(A) NHDFs or SK-N-SHs treated with non-targeting (ctrl), EB1, or CLIP-170 (CLIP) siRNAs were mock-infected or infected with HSV-1 at MOI 10 for 5 h and analyzed by WB. (B) SK-N-SHs treated with the indicated siRNAs were infected, as in (A). (C) SK-N-SHs treated with independent EB2 siRNAs (I or II) were infected, as in (A). (D) SK-N-SHs were treated with 100 nM BafA or DMSO and infected at MOI 10 with VSV for 4 h or HSV-1 for 5 h. (E) NHDFs or SK-N-SHs analyzed by WB using the indicated antibodies. (F) NHDFs or SK-N-SHs stained for tyrosinated (Tyr), detyrosinated (Detyr), and acetylated (Ac) tubulin. Nuclei were stained with Hoechst. (G) NHDFs or SK-N-SHs treated with 500 nM DMSO or 10 μM nocodazole were infected at MOI 20 with HSV-1 for 4 h in the presence of 1 μg/mL actinomycin D. Fixed cells were stained for VP5 and with Hoechst. Assessed for the accumulation of VP5 over 2 biological replicates were ≥ 165 NHDF or ≥ 190 SK-N-SH nuclei; error bars, SEMs; *p < 0.05, **p < 0.01, N.S., not significant; unpaired 2-tailed t test. (H) Cells treated as in (G) were infected at MOI 10 with HSV-1 for 5 h. All of the experiments represent ≥3 replicates unless indicated. See also and .

Journal: Cell reports

Article Title: TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells

doi: 10.1016/j.celrep.2019.12.025

Figure Lengend Snippet: (A) NHDFs or SK-N-SHs treated with non-targeting (ctrl), EB1, or CLIP-170 (CLIP) siRNAs were mock-infected or infected with HSV-1 at MOI 10 for 5 h and analyzed by WB. (B) SK-N-SHs treated with the indicated siRNAs were infected, as in (A). (C) SK-N-SHs treated with independent EB2 siRNAs (I or II) were infected, as in (A). (D) SK-N-SHs were treated with 100 nM BafA or DMSO and infected at MOI 10 with VSV for 4 h or HSV-1 for 5 h. (E) NHDFs or SK-N-SHs analyzed by WB using the indicated antibodies. (F) NHDFs or SK-N-SHs stained for tyrosinated (Tyr), detyrosinated (Detyr), and acetylated (Ac) tubulin. Nuclei were stained with Hoechst. (G) NHDFs or SK-N-SHs treated with 500 nM DMSO or 10 μM nocodazole were infected at MOI 20 with HSV-1 for 4 h in the presence of 1 μg/mL actinomycin D. Fixed cells were stained for VP5 and with Hoechst. Assessed for the accumulation of VP5 over 2 biological replicates were ≥ 165 NHDF or ≥ 190 SK-N-SH nuclei; error bars, SEMs; *p < 0.05, **p < 0.01, N.S., not significant; unpaired 2-tailed t test. (H) Cells treated as in (G) were infected at MOI 10 with HSV-1 for 5 h. All of the experiments represent ≥3 replicates unless indicated. See also and .

Article Snippet: The following antibodies were used for Western Blotting or IF analysis; Abcam: ICP4 (ab6514), ICP0 (ab6513), VSV-G (ab50549), TACC3 for IF (ab134154), Kif5A (ab5628), Kif5B (ab25715), Kif5C (ab5630), α-tubulin (ab18251), γ-tubulin (ab27074), de-tyrosinated-tubulin (ab48389); Cell signaling technologies: β-actin (3700), TACC3 for western (8069S), Flag for IF (15009), Millipore-Sigma: Flag M2 for western and 1 IF (F3165), TACC2 (07–228), acetylated tubulin (T6793), EB3 (AB6033); Life Technologies: EB1 (412100); Santa Cruz Biotechnology, Inc: CLIP-170 (sc-25613); Absea: EB2 (010614A11); Bethyl: Pericentrin (IHC-00264); Bio-Legend: chTOG (620401); Novus Biologicals: TACC1 (NBP189447); Bio-Rad: TGN46 (AHP500GT); Virusys: VP5 (HA018).

Techniques: Infection, Staining

Journal: Cell reports

Article Title: TACC3 Regulates Microtubule Plus-End Dynamics and Cargo Transport in Interphase Cells

doi: 10.1016/j.celrep.2019.12.025

Figure Lengend Snippet:

Article Snippet: The following antibodies were used for Western Blotting or IF analysis; Abcam: ICP4 (ab6514), ICP0 (ab6513), VSV-G (ab50549), TACC3 for IF (ab134154), Kif5A (ab5628), Kif5B (ab25715), Kif5C (ab5630), α-tubulin (ab18251), γ-tubulin (ab27074), de-tyrosinated-tubulin (ab48389); Cell signaling technologies: β-actin (3700), TACC3 for western (8069S), Flag for IF (15009), Millipore-Sigma: Flag M2 for western and 1 IF (F3165), TACC2 (07–228), acetylated tubulin (T6793), EB3 (AB6033); Life Technologies: EB1 (412100); Santa Cruz Biotechnology, Inc: CLIP-170 (sc-25613); Absea: EB2 (010614A11); Bethyl: Pericentrin (IHC-00264); Bio-Legend: chTOG (620401); Novus Biologicals: TACC1 (NBP189447); Bio-Rad: TGN46 (AHP500GT); Virusys: VP5 (HA018).

Techniques: Western Blot, Plasmid Preparation, Recombinant, Transfection, Negative Control, Amplification, Software, Microscopy

Induced Neurons Model Molecular Consequences of CDKL5 Variants but Display NoTranscriptional, Morphological or Functional Changes. A) Normalized log fold gene expression for iN samples. B) Representative western blots for EB2 and phosphorylation of EB2 S221 with TUJ1/Beta-3-tubulin as a loading control. C) Paired t-test of phosphorylation of EB2 fold change relative to EB2 between isogenic control and CDD patient-derived neurons (N=3 for 2 donors). D) Pearson correlation coefficients for gene expression for the 5000 most variable genes between all samples from iNs. E) Volcano plot of differentially expressed genes between pooled isogenic control and CDD patient-derived samples (N=6 per genotype). F) Representative images of iN neurites immunolabeled with TUJ1 and MAP2 in both patient and isogenic control lines. A paired t-test was performed for changes in neurite length normalized to the cell body area (μm/μm 2 ) for G) MAP2 immunofluorescence (N=3 batches). H) Synchrony index and I) weighted mean firing rate for INs during the first 30 days of maturation post-plating (N=4 differentiation batches, 2 donors). Error bars represent the standard error of the mean.

Journal: bioRxiv

Article Title: Excitatory Cortical Neurons from CDKL5 Deficiency Disorder Patient-Derived Organoids Show Early Hyperexcitability Not Identified in Neurogenin2 Induced Neurons

doi: 10.1101/2024.11.11.622878

Figure Lengend Snippet: Induced Neurons Model Molecular Consequences of CDKL5 Variants but Display NoTranscriptional, Morphological or Functional Changes. A) Normalized log fold gene expression for iN samples. B) Representative western blots for EB2 and phosphorylation of EB2 S221 with TUJ1/Beta-3-tubulin as a loading control. C) Paired t-test of phosphorylation of EB2 fold change relative to EB2 between isogenic control and CDD patient-derived neurons (N=3 for 2 donors). D) Pearson correlation coefficients for gene expression for the 5000 most variable genes between all samples from iNs. E) Volcano plot of differentially expressed genes between pooled isogenic control and CDD patient-derived samples (N=6 per genotype). F) Representative images of iN neurites immunolabeled with TUJ1 and MAP2 in both patient and isogenic control lines. A paired t-test was performed for changes in neurite length normalized to the cell body area (μm/μm 2 ) for G) MAP2 immunofluorescence (N=3 batches). H) Synchrony index and I) weighted mean firing rate for INs during the first 30 days of maturation post-plating (N=4 differentiation batches, 2 donors). Error bars represent the standard error of the mean.

Article Snippet: Membranes were then incubated for one hour at room temperature with EB2 1:500 (Abcam ab234843), 1:500 p-EB2 S222 (CovalAb pab01032-P) Signaling Technologies #5364), 1:500 S6 (Santa Cruz #sc-74459), 1:500 p-AKT (Sigma #05-1003), 1:500 AKT (Cell Signaling Technologies #4691S), 1:500 p-GSK (Cell Signaling Technologies #5558), 1:500 GSK (Cell Signaling Technologies #5676S) 1:4000 Acetylated tubulin (Sigma #T7451), 1:4000 TUJ1 (Sigma #T8660), 1:4000 Tyrosinated tubulin (Millipore #MAB 1864), 1:2000 GAPDH (Invitrogen #AM4300), washed by TBS-T, incubated for one hour at room temperature with secondary antibodies at 1:10000 (Alexa Fluor488, ThermoFisher #A28175; Alexa Fluor568, ThermoFisher #A-11011) and imaged using the Odyssey imaging system (LI-COR).

Techniques: Functional Assay, Expressing, Western Blot, Control, Derivative Assay, Immunolabeling, Immunofluorescence

No Altered mTOR Signaling in iNs. A) Representative Western blots for phosphorylation of different mTOR-signaling associated proteins. pEB2 and EB2 as shown in for reference. B) Quantification of normalized protein abundance for each tested protein (N=3). Each shape represents a differentiation batch.

Journal: bioRxiv

Article Title: Excitatory Cortical Neurons from CDKL5 Deficiency Disorder Patient-Derived Organoids Show Early Hyperexcitability Not Identified in Neurogenin2 Induced Neurons

doi: 10.1101/2024.11.11.622878

Figure Lengend Snippet: No Altered mTOR Signaling in iNs. A) Representative Western blots for phosphorylation of different mTOR-signaling associated proteins. pEB2 and EB2 as shown in for reference. B) Quantification of normalized protein abundance for each tested protein (N=3). Each shape represents a differentiation batch.

Article Snippet: Membranes were then incubated for one hour at room temperature with EB2 1:500 (Abcam ab234843), 1:500 p-EB2 S222 (CovalAb pab01032-P) Signaling Technologies #5364), 1:500 S6 (Santa Cruz #sc-74459), 1:500 p-AKT (Sigma #05-1003), 1:500 AKT (Cell Signaling Technologies #4691S), 1:500 p-GSK (Cell Signaling Technologies #5558), 1:500 GSK (Cell Signaling Technologies #5676S) 1:4000 Acetylated tubulin (Sigma #T7451), 1:4000 TUJ1 (Sigma #T8660), 1:4000 Tyrosinated tubulin (Millipore #MAB 1864), 1:2000 GAPDH (Invitrogen #AM4300), washed by TBS-T, incubated for one hour at room temperature with secondary antibodies at 1:10000 (Alexa Fluor488, ThermoFisher #A28175; Alexa Fluor568, ThermoFisher #A-11011) and imaged using the Odyssey imaging system (LI-COR).

Techniques: Western Blot

Cortical ACTX Neurons with CDKL5 Variants Have Reduced pEB2 and Decreased Expression of CDKL5 . A) Representative western blot of EB2 and pEB2 from ACTX neurons. B) Fold change quantification of western blots for phosphorylation of EB2 relative to EB2 across 3 donors (N = 4 donors). C) Pearson correlations across ACTX neuronal differentiations. D) Differentially expressed genes between pooled isogenic controls and CDD proband-derived neurons (N=7 sample per genotype across 3 donors). E) log2 fold change of CDKL5 and HS3ST1 in ACTX neurons of patient cell lines relative to isogenic controls (N=3, paired t-test). EIF2A is the house-keeping gene.

Journal: bioRxiv

Article Title: Excitatory Cortical Neurons from CDKL5 Deficiency Disorder Patient-Derived Organoids Show Early Hyperexcitability Not Identified in Neurogenin2 Induced Neurons

doi: 10.1101/2024.11.11.622878

Figure Lengend Snippet: Cortical ACTX Neurons with CDKL5 Variants Have Reduced pEB2 and Decreased Expression of CDKL5 . A) Representative western blot of EB2 and pEB2 from ACTX neurons. B) Fold change quantification of western blots for phosphorylation of EB2 relative to EB2 across 3 donors (N = 4 donors). C) Pearson correlations across ACTX neuronal differentiations. D) Differentially expressed genes between pooled isogenic controls and CDD proband-derived neurons (N=7 sample per genotype across 3 donors). E) log2 fold change of CDKL5 and HS3ST1 in ACTX neurons of patient cell lines relative to isogenic controls (N=3, paired t-test). EIF2A is the house-keeping gene.

Article Snippet: Membranes were then incubated for one hour at room temperature with EB2 1:500 (Abcam ab234843), 1:500 p-EB2 S222 (CovalAb pab01032-P) Signaling Technologies #5364), 1:500 S6 (Santa Cruz #sc-74459), 1:500 p-AKT (Sigma #05-1003), 1:500 AKT (Cell Signaling Technologies #4691S), 1:500 p-GSK (Cell Signaling Technologies #5558), 1:500 GSK (Cell Signaling Technologies #5676S) 1:4000 Acetylated tubulin (Sigma #T7451), 1:4000 TUJ1 (Sigma #T8660), 1:4000 Tyrosinated tubulin (Millipore #MAB 1864), 1:2000 GAPDH (Invitrogen #AM4300), washed by TBS-T, incubated for one hour at room temperature with secondary antibodies at 1:10000 (Alexa Fluor488, ThermoFisher #A28175; Alexa Fluor568, ThermoFisher #A-11011) and imaged using the Odyssey imaging system (LI-COR).

Techniques: Expressing, Western Blot, Derivative Assay