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  • 91
    STEMCELL Technologies Inc easysep magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Magnet, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 91/100, based on 845 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Becton Dickinson easysep magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Magnet, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep magnet purification
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Magnet Purification, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    STEMCELL Technologies Inc purple easysep magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Purple Easysep Magnet, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 88/100, based on 63 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc silver easysep magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Silver Easysep Magnet, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    STEMCELL Technologies Inc easysep magnet stand
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Magnet Stand, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easyeight easysep magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easyeight Easysep Magnet, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep magnet nanoparticles
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Magnet Nanoparticles, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep column free magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Column Free Magnet, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Thermo Fisher easysep magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Magnet, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Ocean NanoTech easysep magnet
    Expansion of VSELs and their characterization. a The <t>EasySep™</t> purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P
    Easysep Magnet, supplied by Ocean NanoTech, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep magnet kit
    CD34 + CD31 + Endothelial Progenitors Are Multipotent 19-9-11 iPSCs were differentiated as illustrated in Figure 3 A using LaSR basal medium. (A) At day 5, CD34 + cells were enriched with the <t>EasySep</t> Human CD34 Positive Selection Kit and purification quantified by flow cytometry for CD34 expression. (B–D) Sorted CD34 + cells were cultured in a combined medium (B), a smooth muscle medium (C), or an endothelial medium (D) at a density of one cell per well in a 48-well plate for another 10 days. Sample immunofluorescence images for smooth muscle and endothelial markers were shown. In (B), arrows point to SMMHC + VE-cadherin − cells. See also Figure S3 .
    Easysep Magnet Kit, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 86/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep magnet activated cell sorting
    CD34 + CD31 + Endothelial Progenitors Are Multipotent 19-9-11 iPSCs were differentiated as illustrated in Figure 3 A using LaSR basal medium. (A) At day 5, CD34 + cells were enriched with the <t>EasySep</t> Human CD34 Positive Selection Kit and purification quantified by flow cytometry for CD34 expression. (B–D) Sorted CD34 + cells were cultured in a combined medium (B), a smooth muscle medium (C), or an endothelial medium (D) at a density of one cell per well in a 48-well plate for another 10 days. Sample immunofluorescence images for smooth muscle and endothelial markers were shown. In (B), arrows point to SMMHC + VE-cadherin − cells. See also Figure S3 .
    Easysep Magnet Activated Cell Sorting, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 93/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep magnet device
    CD34 + CD31 + Endothelial Progenitors Are Multipotent 19-9-11 iPSCs were differentiated as illustrated in Figure 3 A using LaSR basal medium. (A) At day 5, CD34 + cells were enriched with the <t>EasySep</t> Human CD34 Positive Selection Kit and purification quantified by flow cytometry for CD34 expression. (B–D) Sorted CD34 + cells were cultured in a combined medium (B), a smooth muscle medium (C), or an endothelial medium (D) at a density of one cell per well in a 48-well plate for another 10 days. Sample immunofluorescence images for smooth muscle and endothelial markers were shown. In (B), arrows point to SMMHC + VE-cadherin − cells. See also Figure S3 .
    Easysep Magnet Device, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc swing bucket centrifuge incubated shaker easysep magnet
    CD34 + CD31 + Endothelial Progenitors Are Multipotent 19-9-11 iPSCs were differentiated as illustrated in Figure 3 A using LaSR basal medium. (A) At day 5, CD34 + cells were enriched with the <t>EasySep</t> Human CD34 Positive Selection Kit and purification quantified by flow cytometry for CD34 expression. (B–D) Sorted CD34 + cells were cultured in a combined medium (B), a smooth muscle medium (C), or an endothelial medium (D) at a density of one cell per well in a 48-well plate for another 10 days. Sample immunofluorescence images for smooth muscle and endothelial markers were shown. In (B), arrows point to SMMHC + VE-cadherin − cells. See also Figure S3 .
    Swing Bucket Centrifuge Incubated Shaker Easysep Magnet, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep magnetic beads
    CD34 + CD31 + Endothelial Progenitors Are Multipotent 19-9-11 iPSCs were differentiated as illustrated in Figure 3 A using LaSR basal medium. (A) At day 5, CD34 + cells were enriched with the <t>EasySep</t> Human CD34 Positive Selection Kit and purification quantified by flow cytometry for CD34 expression. (B–D) Sorted CD34 + cells were cultured in a combined medium (B), a smooth muscle medium (C), or an endothelial medium (D) at a density of one cell per well in a 48-well plate for another 10 days. Sample immunofluorescence images for smooth muscle and endothelial markers were shown. In (B), arrows point to SMMHC + VE-cadherin − cells. See also Figure S3 .
    Easysep Magnetic Beads, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 88/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    STEMCELL Technologies Inc easysep magnetic particles
    Dot plot, 3A, and histograms of the settling velocity and magnetic velocity, 3B and 3C, respectively, of <t>EasySep</t> D magnetic particles (Stem Cell Technologies Inc. 19250) using the electromagnet CTV (0.2A; S m = 0.84 TA/mm 3 ).
    Easysep Magnetic Particles, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 88/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Expansion of VSELs and their characterization. a The EasySep™ purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P

    Journal: Stem Cell Reviews

    Article Title: VSELs Maintain their Pluripotency and Competence to Differentiate after Enhanced Ex Vivo Expansion

    doi: 10.1007/s12015-018-9821-1

    Figure Lengend Snippet: Expansion of VSELs and their characterization. a The EasySep™ purified Lin-CD34 + CD45- cord blood cells morphology as observed by optical microscopy (upper panels) or their May-Grünwald Giemsa cytospin preparations (lower panels) at day 9 cultures in media supplemented with DMSO as control or UM171. Annexin V staining of VSELs and total nucleated cells (TNC) after 14 days culture in control media or supplemented with UM171 are shown on the bottom. b The 7AAD + Lin-CD34 + CD45-CD133+ VSELs cell cycle determination by vybrant labeling after 24 h expansion in StemSpan™ containing UM171, in comparison to VSELs cultured in minimal media (conditioned media CM). In the right panels are represented the cell cycles determined from the total nucleated cells (TNCs) in the same conditions of culture than VSELs (representative experiment). C The percentages of CD34+ stem cells and phenotypically defined VSELs subsets NANOG+ and CD133+ calculated after 12 days expansion of Lin-CD34 + CD45- cells. Bars represent average ± SD, n = 4 (* P

    Article Snippet: Cells were then incubated with a cocktail of lineage specific antibodies directed against CD2, CD3, CD11b, CD11c, CD14, CD16, CD19, CD24, CD56, CD61, CD66b, and GlyA; from an EasySep™ progenitor cell enrichment kit with platelet depletion and human CD45 depletion kit (STEMCELL Technologies) for immuno-magnetic negative selection of Lin-CD45- cells using an EasySep™ magnet (STEMCELL Technologies).

    Techniques: Purification, Microscopy, Staining, Labeling, Cell Culture

    Enrichment for nonhaematopoietic cells. Cultured malignant cells were added to 4 ml of whole blood and cells were incubated with tetrameric antibody complexes against CD45 and dextran‐coated magnetic particles for 1 hr and placed in an EasySep Big Easy magnet. Cells not attracted to the magnet were recovered. The number of leukocytes was counted with a haemocytometer prior to and after depletion. Erythrocytes were removed by lysis and platelets by centrifugation at 250 g.

    Journal: International Journal of Cancer

    Article Title: High‐resolution imaging for the detection and characterisation of circulating tumour cells from patients with oesophageal, hepatocellular, thyroid and ovarian cancers

    doi: 10.1002/ijc.29680

    Figure Lengend Snippet: Enrichment for nonhaematopoietic cells. Cultured malignant cells were added to 4 ml of whole blood and cells were incubated with tetrameric antibody complexes against CD45 and dextran‐coated magnetic particles for 1 hr and placed in an EasySep Big Easy magnet. Cells not attracted to the magnet were recovered. The number of leukocytes was counted with a haemocytometer prior to and after depletion. Erythrocytes were removed by lysis and platelets by centrifugation at 250 g.

    Article Snippet: The cell suspension was diluted in 5 ml of Robosep buffer and placed in an EasySep™ big easy magnet (Stemcell Technologies, UK) for 10 min at room temperature.

    Techniques: Cell Culture, Incubation, Lysis, Centrifugation

    Discrimination of the malignant cell population in whole blood from the residual leukocytes after positive depletion of blood cells. Cultured malignant SK‐GT‐4 cells were added to 4 ml of whole blood. Erythrocytes were removed by ammonium chloride lysis and platelets by centrifugation at 250 g. The cells in the supernatant were incubated with tetrameric antibody complexes against CD45 and dextran‐coated magnetic particles for 1 hr and placed in an EasySep Big Easy magnet. The cells not attracted to the magnet were recovered and analysed in an ImageStream X flow cytometer as described in the legend to Figure 1. The number of images at each DAPI fluorescence intensity is shown ( a ). The intensity of fluorescence for the CD45 antibody is compared with the intensity of fluorescence of the EpCAM antibody for all cells distinguished ( b ).

    Journal: International Journal of Cancer

    Article Title: High‐resolution imaging for the detection and characterisation of circulating tumour cells from patients with oesophageal, hepatocellular, thyroid and ovarian cancers

    doi: 10.1002/ijc.29680

    Figure Lengend Snippet: Discrimination of the malignant cell population in whole blood from the residual leukocytes after positive depletion of blood cells. Cultured malignant SK‐GT‐4 cells were added to 4 ml of whole blood. Erythrocytes were removed by ammonium chloride lysis and platelets by centrifugation at 250 g. The cells in the supernatant were incubated with tetrameric antibody complexes against CD45 and dextran‐coated magnetic particles for 1 hr and placed in an EasySep Big Easy magnet. The cells not attracted to the magnet were recovered and analysed in an ImageStream X flow cytometer as described in the legend to Figure 1. The number of images at each DAPI fluorescence intensity is shown ( a ). The intensity of fluorescence for the CD45 antibody is compared with the intensity of fluorescence of the EpCAM antibody for all cells distinguished ( b ).

    Article Snippet: The cell suspension was diluted in 5 ml of Robosep buffer and placed in an EasySep™ big easy magnet (Stemcell Technologies, UK) for 10 min at room temperature.

    Techniques: Cell Culture, Lysis, Centrifugation, Incubation, Flow Cytometry, Cytometry, Fluorescence

    CD34 + CD31 + Endothelial Progenitors Are Multipotent 19-9-11 iPSCs were differentiated as illustrated in Figure 3 A using LaSR basal medium. (A) At day 5, CD34 + cells were enriched with the EasySep Human CD34 Positive Selection Kit and purification quantified by flow cytometry for CD34 expression. (B–D) Sorted CD34 + cells were cultured in a combined medium (B), a smooth muscle medium (C), or an endothelial medium (D) at a density of one cell per well in a 48-well plate for another 10 days. Sample immunofluorescence images for smooth muscle and endothelial markers were shown. In (B), arrows point to SMMHC + VE-cadherin − cells. See also Figure S3 .

    Journal: Stem Cell Reports

    Article Title: Efficient Differentiation of Human Pluripotent Stem Cells to Endothelial Progenitors via Small-Molecule Activation of WNT Signaling

    doi: 10.1016/j.stemcr.2014.09.005

    Figure Lengend Snippet: CD34 + CD31 + Endothelial Progenitors Are Multipotent 19-9-11 iPSCs were differentiated as illustrated in Figure 3 A using LaSR basal medium. (A) At day 5, CD34 + cells were enriched with the EasySep Human CD34 Positive Selection Kit and purification quantified by flow cytometry for CD34 expression. (B–D) Sorted CD34 + cells were cultured in a combined medium (B), a smooth muscle medium (C), or an endothelial medium (D) at a density of one cell per well in a 48-well plate for another 10 days. Sample immunofluorescence images for smooth muscle and endothelial markers were shown. In (B), arrows point to SMMHC + VE-cadherin − cells. See also Figure S3 .

    Article Snippet: Differentiation of CD34+ Cells to Smooth Muscle Cells Day 5 differentiated populations were dissociated with Accutase for 10 min and purified with an EasySep Magnet kit (STEMCELL Technologies) using a CD34 antibody according to the manufacturer’s instructions.

    Techniques: Selection, Purification, Flow Cytometry, Cytometry, Expressing, Cell Culture, Immunofluorescence

    Dot plot, 3A, and histograms of the settling velocity and magnetic velocity, 3B and 3C, respectively, of EasySep D magnetic particles (Stem Cell Technologies Inc. 19250) using the electromagnet CTV (0.2A; S m = 0.84 TA/mm 3 ).

    Journal: Journal of magnetism and magnetic materials

    Article Title: Simultaneous, single particle, magnetization and size measurements of micron sized, magnetic particles

    doi: 10.1016/j.jmmm.2012.07.039

    Figure Lengend Snippet: Dot plot, 3A, and histograms of the settling velocity and magnetic velocity, 3B and 3C, respectively, of EasySep D magnetic particles (Stem Cell Technologies Inc. 19250) using the electromagnet CTV (0.2A; S m = 0.84 TA/mm 3 ).

    Article Snippet: To provide independent measurements of particle diameter (relative to the CTV system), a microscopic, digital imaging system (Nikon 80i microscope and the NIS Elements BR research software) was used. (It should be noted that a significant number of particles are tracked by the CTV system that are out of focus; hence, independent particle size measurements were conducted in the Nikon microscope which placed the particles on a slide in the same focal plane). is a histogram of the diameter, in microns, of the Stem Cell Technologies EasySep™ magnetic particles (sample taken from same vial as used to make ).

    Techniques: