Journal: Biomolecules

Article Title: Crinamine Induces Apoptosis and Inhibits Proliferation, Migration, and Angiogenesis in Cervical Cancer SiHa Cells

doi: 10.3390/biom9090494

Figure Lengend Snippet: Crinamine-mediated apoptosis activation in cervical cancer cells. ( a ) Annexin V and PI staining of SiHa cells treated with cisplatin, crinamine, or DMSO for 16 h. ( b ) Fluorescent intensity of Annexin V staining from ( a ); results are shown as means ± SD from triplicate measurements. ( c ) Caspase-3/7 activity of crinamine in SiHa cells non-treated or treated with the indicated concentrations of cisplatin or crinamine for 24 h. Caspase activity is normalized to the DMSO control and is reported as means ± SD from triplicate experiments. ( d ) Immunofluorescence staining of histone γ-H2AX in SiHa cells treated with the indicated concentrations of cisplatin, crinamine, or DMSO for 4 and 24 h. Panels represent phase contrast (PhL) images, DAPI nuclear staining (blue), γ-H2AX foci (red), and merged images. ( e ) Percentage of γ-H2AX foci-positive cells quantified from five fields per treatment obtained from duplicate measurements. Results are representative of data observed on two separate occasions. * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: SiHa cells were seeded in 24-well plates and treated with the indicated concentration of cisplatin, crinamine, or 0.16% DMSO for 16 h. Cells were stained with Annexin V-fluorescein isothiocyanate (FITC) using the Annexin V-FITC Early Apoptosis Detection Kit (#6592; Cell Signaling Technology, Danvers, MA, USA).

Techniques: Activation Assay, Staining, Activity Assay, Immunofluorescence

Journal: Cancer Science

Article Title: Interleukin‐6 production mediated by the IRE 1‐ XBP 1 pathway confers radioresistance in human papillomavirus‐negative oropharyngeal carcinoma

doi: 10.1111/cas.14094

Figure Lengend Snippet: Schematic model of IRE 1‐ XBP 1‐induced radioresistance via interleukin‐6 ( IL ‐6) upregulation. Exposure to X‐ray irradiation causes endoplasmic reticulum stress (ERS) , and increased levels of IRE 1 promote the splicing of XBP 1 mRNA . The activated transcription factor XBP 1s translocates to the nucleus and induces the expression of IL ‐6. Concurrently, the formation of DNA double‐strand breaks (DSB) is inhibited and helps decrease apoptosis, which is related to radioresistance

Article Snippet: Experiments were performed according to the protocol supplied with the Annexin‐Green Apoptosis cell detection reagent kit (Cell Signaling Technology).

Techniques: Irradiation, Expressing

Journal: Drug Delivery

Article Title: A recombinant scFv antibody-based fusion protein that targets EGFR associated with IMPDH2 downregulation and its drug conjugate show therapeutic efficacy against esophageal cancer

doi: 10.1080/10717544.2022.2063454

Figure Lengend Snippet: DNA damage, cell cycle arrest analysis, and apoptosis of EC cells. (A,B) Flow cytometry analyses of apoptosis in KYSE150 and Eca109 cells treated with Fv-LDP-D3 and Fv-LDP-D3-AE, respectively. (C) Western blot analysis of apoptosis-related proteins in KYSE150 cells treated with Fv-LDP-D3. (D) Western blot analysis of apoptosis- and DNA damage-related protein expression in KYSE150 and Eca109 cells treated with Fv-LDP-D3-AE. (E) Cell cycle arrest in KYSE150 and Eca109 cells treated with Fv-LDP-D3-AE was determined by flow cytometry. Results are reported as means ± standard deviation (SD) ( n = 3). ** p < 0.01, *** p < 0.001 versus control group.

Article Snippet: Cells were collected according to the instructions of the Annexin V FITC Apoptosis Detection Kit (Dojindo).

Techniques: Flow Cytometry, Western Blot, Expressing, Standard Deviation