ea.hy926 genomic dna Search Results


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    <t>RT-PCR</t> analysis of ACE3 mRNA expression in various murine tissues . Total <t>RNA</t> (1 μg) from the indicated tissues was subjected to one step RT-PCR as described in Materials and Methods followed by electrophoresis on a 1% agarose gel. (A) mouse ACE3; (B) actin. Lanes are as follows: 1, positive control (whole mouse RNA with primers for actin); 2, negative control (no RNA); 3, brain; 4, embryo; 5, heart; 6, kidney; 7, liver; 8, lung; 9, ovary; 10, spleen; 11, testis; 12, thymus.
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    <t>RT-PCR</t> analysis of ACE3 mRNA expression in various murine tissues . Total <t>RNA</t> (1 μg) from the indicated tissues was subjected to one step RT-PCR as described in Materials and Methods followed by electrophoresis on a 1% agarose gel. (A) mouse ACE3; (B) actin. Lanes are as follows: 1, positive control (whole mouse RNA with primers for actin); 2, negative control (no RNA); 3, brain; 4, embryo; 5, heart; 6, kidney; 7, liver; 8, lung; 9, ovary; 10, spleen; 11, testis; 12, thymus.
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    Image Search Results


    RT-PCR analysis of ACE3 mRNA expression in various murine tissues . Total RNA (1 μg) from the indicated tissues was subjected to one step RT-PCR as described in Materials and Methods followed by electrophoresis on a 1% agarose gel. (A) mouse ACE3; (B) actin. Lanes are as follows: 1, positive control (whole mouse RNA with primers for actin); 2, negative control (no RNA); 3, brain; 4, embryo; 5, heart; 6, kidney; 7, liver; 8, lung; 9, ovary; 10, spleen; 11, testis; 12, thymus.

    Journal: BMC Genomics

    Article Title: Identification and characterisation of the angiotensin converting enzyme-3 (ACE3) gene: a novel mammalian homologue of ACE

    doi: 10.1186/1471-2164-8-194

    Figure Lengend Snippet: RT-PCR analysis of ACE3 mRNA expression in various murine tissues . Total RNA (1 μg) from the indicated tissues was subjected to one step RT-PCR as described in Materials and Methods followed by electrophoresis on a 1% agarose gel. (A) mouse ACE3; (B) actin. Lanes are as follows: 1, positive control (whole mouse RNA with primers for actin); 2, negative control (no RNA); 3, brain; 4, embryo; 5, heart; 6, kidney; 7, liver; 8, lung; 9, ovary; 10, spleen; 11, testis; 12, thymus.

    Article Snippet: Reverse transcription-PCR Human and murine (from Swiss Webster mice) total RNA was from Ambion (Europe) Ltd. (Huntingdon, UK).

    Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Electrophoresis, Agarose Gel Electrophoresis, Positive Control, Negative Control