e. faecium strains Search Results


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  • 96
    ATCC e faecium strains
    PCR-HRMA difference plots of VRE and VSE E faecalis and E <t>faecium</t> generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.
    E Faecium Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Millipore e faecium l50 14
    PCR-HRMA difference plots of VRE and VSE E faecalis and E <t>faecium</t> generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.
    E Faecium L50 14, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    ATCC e faecium strains ge 1
    PCR-HRMA difference plots of VRE and VSE E faecalis and E <t>faecium</t> generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.
    E Faecium Strains Ge 1, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e faecium strains atcc 700221
    a 16S rRNA nucleotide sequence alignment of Lactococcus lactis isolates S2 and S6 with the sequence of Lactococcus lactis subsp. lactic ATCC 19435 (upper and middle panels) and with Lactococcus lactis subsp. lactis IL1403 (lower panel) (nucleotide differences are highlighted). b 16S rRNA nucleotide sequence alignment of Enterococcus <t>faecium</t> isolate S11 with the sequence of Enterococcus faecium type strains ATCC 700221, ATCC 19434, CECT 410 T and DSM 20477 (nucleotide differences are highlighted)
    E Faecium Strains Atcc 700221, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    BEI Resources e faecium strain 513
    a 16S rRNA nucleotide sequence alignment of Lactococcus lactis isolates S2 and S6 with the sequence of Lactococcus lactis subsp. lactic ATCC 19435 (upper and middle panels) and with Lactococcus lactis subsp. lactis IL1403 (lower panel) (nucleotide differences are highlighted). b 16S rRNA nucleotide sequence alignment of Enterococcus <t>faecium</t> isolate S11 with the sequence of Enterococcus faecium type strains ATCC 700221, ATCC 19434, CECT 410 T and DSM 20477 (nucleotide differences are highlighted)
    E Faecium Strain 513, supplied by BEI Resources, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    BGI Shenzhen e faecium strain lct ef301
    a 16S rRNA nucleotide sequence alignment of Lactococcus lactis isolates S2 and S6 with the sequence of Lactococcus lactis subsp. lactic ATCC 19435 (upper and middle panels) and with Lactococcus lactis subsp. lactis IL1403 (lower panel) (nucleotide differences are highlighted). b 16S rRNA nucleotide sequence alignment of Enterococcus <t>faecium</t> isolate S11 with the sequence of Enterococcus faecium type strains ATCC 700221, ATCC 19434, CECT 410 T and DSM 20477 (nucleotide differences are highlighted)
    E Faecium Strain Lct Ef301, supplied by BGI Shenzhen, used in various techniques. Bioz Stars score: 86/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    84
    ATCC e faecium atcc 6569 strain
    Effect of the antibiotic activity of Fluopsin C on the ultra-structural of the Gram-positive bacteria Enterococcus <t>faecium</t> strain ATCC 6569. Scanning electron microscopy: (A,B) control (non-treated) – (A) 8,000× and (B) (40,000×); (C,D) cells treated with Fluopsin C – (C) (8,000×) and (D) (40,000×).
    E Faecium Atcc 6569 Strain, supplied by ATCC, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Roche probiotic strain e faecium ncimb 10415
    Effect of the antibiotic activity of Fluopsin C on the ultra-structural of the Gram-positive bacteria Enterococcus <t>faecium</t> strain ATCC 6569. Scanning electron microscopy: (A,B) control (non-treated) – (A) 8,000× and (B) (40,000×); (C,D) cells treated with Fluopsin C – (C) (8,000×) and (D) (40,000×).
    Probiotic Strain E Faecium Ncimb 10415, supplied by Roche, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    DSM NV probiotic strain e faecium ncimb 10415
    Effect of the antibiotic activity of Fluopsin C on the ultra-structural of the Gram-positive bacteria Enterococcus <t>faecium</t> strain ATCC 6569. Scanning electron microscopy: (A,B) control (non-treated) – (A) 8,000× and (B) (40,000×); (C,D) cells treated with Fluopsin C – (C) (8,000×) and (D) (40,000×).
    Probiotic Strain E Faecium Ncimb 10415, supplied by DSM NV, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC e faecium atcc 35667 strains
    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus <t>faecium</t> ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min
    E Faecium Atcc 35667 Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    95
    ATCC e faecium atcc 51559 strains
    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus <t>faecium</t> ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min
    E Faecium Atcc 51559 Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC e faecium atcc 27270 strains
    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus <t>faecium</t> ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min
    E Faecium Atcc 27270 Strains, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    ATCC target strain e faecium atcc 19443
    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus <t>faecium</t> ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min
    Target Strain E Faecium Atcc 19443, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    ATCC antibiotic susceptible e faecium strain atcc 49624
    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus <t>faecium</t> ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min
    Antibiotic Susceptible E Faecium Strain Atcc 49624, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    ATCC vancomycin susceptible e faecium vse strain atcc 19950
    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus <t>faecium</t> ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min
    Vancomycin Susceptible E Faecium Vse Strain Atcc 19950, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains ( E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains ( E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Polymerase Chain Reaction, Generated

    PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains ( E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains ( E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control

    E. faecium in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P

    Journal: Genome Biology

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host

    doi: 10.1186/s13059-019-1879-9

    Figure Lengend Snippet: E. faecium in the gut causes colitis in Il10 −/− mice. Fecal transplantation from selected subjects (HD55 and IBD36) and inoculation of E. faecium strain ATCC 19434 was performed in microbiota-depleted Il10 −/− mice. The control group was treated with antibiotics (VCM/DRPM) in the absence of transplantation. a Changes in body weight (%) throughout the course of the experiment and b on day 28. c Representative histological sections of the murine colon at the time of euthanasia. Bars, 100 μm. d Mean pathology scores for each group of mice. †, average pathology score of 0. e mRNA expression levels of inflammatory cytokines in the colon as analyzed by real-time PCR. Values shown in a , b , d , and e are the mean ± SE. Statistical differences between two values were analyzed using a Mann-Whitney U test. * P

    Article Snippet: Unlike isolates derived from healthy donors, E. faecium isolates from the feces of UC patients, along with E. faecium strain ATCC 19434, promotes colitis and colonic cytokine expression.

    Techniques: Mouse Assay, Transplantation Assay, Expressing, Real-time Polymerase Chain Reaction, MANN-WHITNEY

    Genome analysis of 10 E. faecium strains reveals inflammatory and probiotic clusters. a Three (HD26a, HD50a, and HD59a) and 4 (IB6a, IB18a, IB44a, and IB50a) E. faecium strains were isolated from the feces of HD subjects and UC patients, respectively. The genotypes of 10 E. faecium strains, including the 3 HD-derived and 4 UC-derived strains, inflammatory strain ATCC 19434, and probiotic strains NCIMB 11181 and SF68, were examined by sequencing. All 1683 identified genes (except for those coding for hypothetical proteins) were used for hierarchical clustering analysis of the 10 E. faecium strains. b LDA was performed using LEfSe to identify significant differences in KEGG-based metabolic pathways in the genomes of the 10 strains to compare between the inflammatory cluster in which ATCC 19434 was included and the probiotic cluster in which NCIMB 11181 and SF68 were included. Differentially abundant pathways for which the corresponding LDA scores indicate P

    Journal: Genome Biology

    Article Title: Gut-derived Enterococcus faecium from ulcerative colitis patients promotes colitis in a genetically susceptible mouse host

    doi: 10.1186/s13059-019-1879-9

    Figure Lengend Snippet: Genome analysis of 10 E. faecium strains reveals inflammatory and probiotic clusters. a Three (HD26a, HD50a, and HD59a) and 4 (IB6a, IB18a, IB44a, and IB50a) E. faecium strains were isolated from the feces of HD subjects and UC patients, respectively. The genotypes of 10 E. faecium strains, including the 3 HD-derived and 4 UC-derived strains, inflammatory strain ATCC 19434, and probiotic strains NCIMB 11181 and SF68, were examined by sequencing. All 1683 identified genes (except for those coding for hypothetical proteins) were used for hierarchical clustering analysis of the 10 E. faecium strains. b LDA was performed using LEfSe to identify significant differences in KEGG-based metabolic pathways in the genomes of the 10 strains to compare between the inflammatory cluster in which ATCC 19434 was included and the probiotic cluster in which NCIMB 11181 and SF68 were included. Differentially abundant pathways for which the corresponding LDA scores indicate P

    Article Snippet: Unlike isolates derived from healthy donors, E. faecium isolates from the feces of UC patients, along with E. faecium strain ATCC 19434, promotes colitis and colonic cytokine expression.

    Techniques: Isolation, Derivative Assay, Sequencing

    (a) Representative comparison of the average spectra of the vanB -positive E. faecium isolates (green) and the vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (red) between 5,916 Da and 6072 Da; (b) corresponding gel view representation (gray-scale color scheme) of the same region. vanB -positive E. faecium isolates (bottom) and vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (top). The peak with a mass of 5,945 Da is peak 28 used in the classification model. arb. u., arbitrary units; Sp. #, spectrum number.

    Journal: Journal of Clinical Microbiology

    Article Title: Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Identify Vancomycin-Resistant Enterococci and Investigate the Epidemiology of an Outbreak

    doi: 10.1128/JCM.01000-12

    Figure Lengend Snippet: (a) Representative comparison of the average spectra of the vanB -positive E. faecium isolates (green) and the vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (red) between 5,916 Da and 6072 Da; (b) corresponding gel view representation (gray-scale color scheme) of the same region. vanB -positive E. faecium isolates (bottom) and vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (top). The peak with a mass of 5,945 Da is peak 28 used in the classification model. arb. u., arbitrary units; Sp. #, spectrum number.

    Article Snippet: The VRE-negative E. faecium control strain ATCC 19434 was identified as Enterococcus faecium 11037_CHB 10 out of 11 times analyzed (91%) and as Enterococcus faecium 20218_1 CHB once (9%), with an average score of 2.40.

    Techniques: Polymerase Chain Reaction

    (a) Representative comparison of the average spectra of the vanB -positive E. faecium isolates (green) and the vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (red) between 2,160 Da and 2,720 Da; (b) corresponding gel view representation (rainbow-scale color scheme) of the same region. The peak with a mass of 2,211 Da is peak 2 used in the classification model. arb. u., arbitrary units; Sp. #, spectrum number.

    Journal: Journal of Clinical Microbiology

    Article Title: Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Identify Vancomycin-Resistant Enterococci and Investigate the Epidemiology of an Outbreak

    doi: 10.1128/JCM.01000-12

    Figure Lengend Snippet: (a) Representative comparison of the average spectra of the vanB -positive E. faecium isolates (green) and the vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (red) between 2,160 Da and 2,720 Da; (b) corresponding gel view representation (rainbow-scale color scheme) of the same region. The peak with a mass of 2,211 Da is peak 2 used in the classification model. arb. u., arbitrary units; Sp. #, spectrum number.

    Article Snippet: The VRE-negative E. faecium control strain ATCC 19434 was identified as Enterococcus faecium 11037_CHB 10 out of 11 times analyzed (91%) and as Enterococcus faecium 20218_1 CHB once (9%), with an average score of 2.40.

    Techniques: Polymerase Chain Reaction

    (a) Representative comparison of the average spectra of the vanB -positive E. faecium isolates (green) and the vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (red) between 5,000 Da and 5,240 Da; (b) corresponding gel view representation (blue-scale color scheme) of the same region. vanB -positive E. faecium isolates (bottom) and vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (top). The peak with a mass of 5,095 Da is peak 23 used in the classification model and is essentially not present in the control samples. arb. u., arbitrary units; Sp. #, spectrum number.

    Journal: Journal of Clinical Microbiology

    Article Title: Use of Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry To Identify Vancomycin-Resistant Enterococci and Investigate the Epidemiology of an Outbreak

    doi: 10.1128/JCM.01000-12

    Figure Lengend Snippet: (a) Representative comparison of the average spectra of the vanB -positive E. faecium isolates (green) and the vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (red) between 5,000 Da and 5,240 Da; (b) corresponding gel view representation (blue-scale color scheme) of the same region. vanB -positive E. faecium isolates (bottom) and vanB -negative E. faecium isolates (isolates phenotypically suspected of being VRE yet PCR negative and control strain ATCC 19434) (top). The peak with a mass of 5,095 Da is peak 23 used in the classification model and is essentially not present in the control samples. arb. u., arbitrary units; Sp. #, spectrum number.

    Article Snippet: The VRE-negative E. faecium control strain ATCC 19434 was identified as Enterococcus faecium 11037_CHB 10 out of 11 times analyzed (91%) and as Enterococcus faecium 20218_1 CHB once (9%), with an average score of 2.40.

    Techniques: Polymerase Chain Reaction

    a 16S rRNA nucleotide sequence alignment of Lactococcus lactis isolates S2 and S6 with the sequence of Lactococcus lactis subsp. lactic ATCC 19435 (upper and middle panels) and with Lactococcus lactis subsp. lactis IL1403 (lower panel) (nucleotide differences are highlighted). b 16S rRNA nucleotide sequence alignment of Enterococcus faecium isolate S11 with the sequence of Enterococcus faecium type strains ATCC 700221, ATCC 19434, CECT 410 T and DSM 20477 (nucleotide differences are highlighted)

    Journal: BMC Microbiology

    Article Title: Diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on Listeria monocytogenes and Salmonella enterica serovar enteritidis in a co-culture model

    doi: 10.1186/s12866-017-0944-3

    Figure Lengend Snippet: a 16S rRNA nucleotide sequence alignment of Lactococcus lactis isolates S2 and S6 with the sequence of Lactococcus lactis subsp. lactic ATCC 19435 (upper and middle panels) and with Lactococcus lactis subsp. lactis IL1403 (lower panel) (nucleotide differences are highlighted). b 16S rRNA nucleotide sequence alignment of Enterococcus faecium isolate S11 with the sequence of Enterococcus faecium type strains ATCC 700221, ATCC 19434, CECT 410 T and DSM 20477 (nucleotide differences are highlighted)

    Article Snippet: Its sequences showed 99% identity to the 16S rRNA gene sequences of E. faecium strains ATCC 700221, ATCC 19434, CECT410T and DSM 20477.

    Techniques: Sequencing

    Effect of the antibiotic activity of Fluopsin C on the ultra-structural of the Gram-positive bacteria Enterococcus faecium strain ATCC 6569. Scanning electron microscopy: (A,B) control (non-treated) – (A) 8,000× and (B) (40,000×); (C,D) cells treated with Fluopsin C – (C) (8,000×) and (D) (40,000×).

    Journal: Frontiers in Microbiology

    Article Title: Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria

    doi: 10.3389/fmicb.2020.01076

    Figure Lengend Snippet: Effect of the antibiotic activity of Fluopsin C on the ultra-structural of the Gram-positive bacteria Enterococcus faecium strain ATCC 6569. Scanning electron microscopy: (A,B) control (non-treated) – (A) 8,000× and (B) (40,000×); (C,D) cells treated with Fluopsin C – (C) (8,000×) and (D) (40,000×).

    Article Snippet: In E. faecium ATCC 6569 strain, despite the decrease in cell numbers after treatment with Fluopsin C, no damage was detected in its cell membrane and wall.

    Techniques: Activity Assay, Electron Microscopy

    Percentage of Enterococcus faecium ATCC 6569 and Staphylococcus aureus ATCC 29213 cells with permeabilized membrane caused by the exposure to F4A and fluopsin. Negative control consists of untreated cells, and positive control consists of cells permeabilized by nisin.

    Journal: Frontiers in Microbiology

    Article Title: Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria

    doi: 10.3389/fmicb.2020.01076

    Figure Lengend Snippet: Percentage of Enterococcus faecium ATCC 6569 and Staphylococcus aureus ATCC 29213 cells with permeabilized membrane caused by the exposure to F4A and fluopsin. Negative control consists of untreated cells, and positive control consists of cells permeabilized by nisin.

    Article Snippet: In E. faecium ATCC 6569 strain, despite the decrease in cell numbers after treatment with Fluopsin C, no damage was detected in its cell membrane and wall.

    Techniques: Negative Control, Positive Control

    F4A and Fluopsin C permeabilized the membrane of E. faecium ATCC 6569. Cells were exposed to the compounds at their respective IC 90 values for 15 min, and after staining with DAPI/IP before microscope observation. Cells with intact membrane are shown in blue, while cells with disrupted membrane are in red. (A) non-treated cells; (B) positive control, cells treated with nisin at 2.5 μg/mL; (C) cells treated with Fluopsin C; (D) cells treated with F4A fraction. Images are the overlay of phase-contrast microscopy and fluorescence. The filters Tx Red and DAPI Blue were applied together and used to visualize IP and DAPI. The bars correspond to 5 μm; magnification of 100X.

    Journal: Frontiers in Microbiology

    Article Title: Determining the Targets of Fluopsin C Action on Gram-Negative and Gram-Positive Bacteria

    doi: 10.3389/fmicb.2020.01076

    Figure Lengend Snippet: F4A and Fluopsin C permeabilized the membrane of E. faecium ATCC 6569. Cells were exposed to the compounds at their respective IC 90 values for 15 min, and after staining with DAPI/IP before microscope observation. Cells with intact membrane are shown in blue, while cells with disrupted membrane are in red. (A) non-treated cells; (B) positive control, cells treated with nisin at 2.5 μg/mL; (C) cells treated with Fluopsin C; (D) cells treated with F4A fraction. Images are the overlay of phase-contrast microscopy and fluorescence. The filters Tx Red and DAPI Blue were applied together and used to visualize IP and DAPI. The bars correspond to 5 μm; magnification of 100X.

    Article Snippet: In E. faecium ATCC 6569 strain, despite the decrease in cell numbers after treatment with Fluopsin C, no damage was detected in its cell membrane and wall.

    Techniques: Staining, Microscopy, Positive Control, Fluorescence

    Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min

    Journal: BMC Microbiology

    Article Title: Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains

    doi: 10.1186/s12866-018-1321-6

    Figure Lengend Snippet: Assessment of metabolic activity of biofilm cells by resazurin. Quantification of biofilm production of six bacterial strains, using three concentrations of resazurin solution (2, 4 and 8 μg/mL): ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212, ( F ) Enterococcus faecalis VRE ATCC 51575, incubated at 25 °C and 37 °C. Relative fluorescence measurements were taken at 20, 40, 60 and 80 min

    Article Snippet: It was observed that supplementing TS broth with glucose increased the biofilm production of S. aureus and E. faecium ATCC 35667 strains (Fig. ), although a significant difference was only observed for the methicillin-resistant S. aureus (MRSA) strain (P < 0.05) (Fig. ).

    Techniques: Activity Assay, Incubation, Fluorescence

    Assessment of biofilm biomass by crystal violet staining and cell enumeration by colony count. Biofilm production analysis through crystal violet staining (OD 570 nm, bars) and cell enumeration (log 10 CFU/mL, lines) of six bacterial strains: ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212 and ( f ) Enterococcus faecalis VRE ATCC 51575, incubated in six different media: Mueller Hinton (MH), Tryptic Soy (TS), Tryptic Soy supplemented with 1% glucose (TSG), Tryptic Soy supplemented with 2% glucose (TS2G), Brain Heart Infusion (BHI) and Brain Heart Infusion supplemented with 1% glucose (BHIG). Error bars represent standard deviation of two independent experiments in triplicate. Lowercase letters indicate significant differences amongst media used for biofilm production. Similar letters denote no significant difference ( P > 0.05)

    Journal: BMC Microbiology

    Article Title: Defining conditions for biofilm inhibition and eradication assays for Gram-positive clinical reference strains

    doi: 10.1186/s12866-018-1321-6

    Figure Lengend Snippet: Assessment of biofilm biomass by crystal violet staining and cell enumeration by colony count. Biofilm production analysis through crystal violet staining (OD 570 nm, bars) and cell enumeration (log 10 CFU/mL, lines) of six bacterial strains: ( a ) Staphylococcus aureus ATCC 29213, ( b ) Staphylococcus aureus MRSA ATCC 43300, ( c ) Enterococcus faecium ATCC 35667, ( d ) Enterococcus faecium VRE ATCC 700221, ( e ) Enterococcus faecalis ATCC 29212 and ( f ) Enterococcus faecalis VRE ATCC 51575, incubated in six different media: Mueller Hinton (MH), Tryptic Soy (TS), Tryptic Soy supplemented with 1% glucose (TSG), Tryptic Soy supplemented with 2% glucose (TS2G), Brain Heart Infusion (BHI) and Brain Heart Infusion supplemented with 1% glucose (BHIG). Error bars represent standard deviation of two independent experiments in triplicate. Lowercase letters indicate significant differences amongst media used for biofilm production. Similar letters denote no significant difference ( P > 0.05)

    Article Snippet: It was observed that supplementing TS broth with glucose increased the biofilm production of S. aureus and E. faecium ATCC 35667 strains (Fig. ), although a significant difference was only observed for the methicillin-resistant S. aureus (MRSA) strain (P < 0.05) (Fig. ).

    Techniques: Staining, Incubation, Standard Deviation