e. faecalis Search Results


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  • 99
    ATCC e faecalis
    SEM images of E. <t>faecalis</t> biofilm on the root canal walls and inside the dentinal tubules (a) ×2000 maginification, (b) ×3000 magnification, (c) ×5000 magnification, (d) ×8000 magnification
    E Faecalis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2789 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Difco e faecalis
    In vitro analysis of Ef -OAD stability as a function of pH. E. <t>faecalis</t> cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4
    E Faecalis, supplied by Difco, used in various techniques. Bioz Stars score: 92/100, based on 156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Bio-Rad e faecalis
    Effect of the deletion of the ef_3196/7 operon on virulence. Deletion mutant of the in vivo induced genes encoding a two-component system ( ef_3196/7 operon) has been constructed. Surviving G. mellonella larvae are counted from 16 to 24 hours after infection with E. <t>faecalis</t> V19 (black bar) and E. faecalis ΔTCS (white bar). Bars indicated by an asterisk show a significant difference in comparison with wild type (*, p≤0.05). Experiments were repeated at least three times.
    E Faecalis, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 92/100, based on 159 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    ATCC vancomycin resistant e faecalis
    Effect of the deletion of the ef_3196/7 operon on virulence. Deletion mutant of the in vivo induced genes encoding a two-component system ( ef_3196/7 operon) has been constructed. Surviving G. mellonella larvae are counted from 16 to 24 hours after infection with E. <t>faecalis</t> V19 (black bar) and E. faecalis ΔTCS (white bar). Bars indicated by an asterisk show a significant difference in comparison with wild type (*, p≤0.05). Experiments were repeated at least three times.
    Vancomycin Resistant E Faecalis, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 82 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    HiMedia Laboratories e faecalis
    Comparison of inhibition zones of control group A vs . Experimental groups B, C, and D. (A) For all tested organisms; (B) for E. <t>faecalis</t> ; (C) for S. aureus ; (D) for C. albicans. Group A (control), sodium hypochlorite; group B (OT), obicure tea extract; group C (LG), lemon grass oil; group D (BO), basil oil.
    E Faecalis, supplied by HiMedia Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Clinical and Laboratory Standards Institute e faecalis
    In vitro antibiofilm and antibacterial activity of Enterococcus <t>faecalis</t> : ( A ) BA and derivatives; ( B ) UA and derivatives. The results represent the mean ± standard deviation of five experiments. * Represents a significant difference in relation to the biofilm formation control ( p
    E Faecalis, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 91/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Merck KGaA e faecalis
    Genetic organization of ORFs in the contig harboring the tetracycline resistance gene tet (S) from the genome sequence of S. thermophilus St-9 (A) and in the contig harboring the erythromycin resistance gene ermB from the genome of S. thermophilus St-5 (B) . Color code of the ORFs: in yellow, ORFs complete and/or incomplete ORFs related to transposases, invertases, and topoisomerases; in purple, ORFs involved in antibiotic resistance; in light blue, ORFs related to plasmid segregation and stability; in green, ORF involved in conjugation/mobilization; in white, ORFs encoding hypothetical proteins or proteins coding for other systems. Specific features of (B) : (i) the position of oriT is also indicated; (ii) arrowheads represent a region of direct repeats (DR) in front of the replication module, which showed no significant homology to those in pRE25 and pSM19035; (iii) long segments of the contig (black bars) highly similar ( > 99% nucleotide identity) or identical to plasmid sequences of pRE25 from Enterococcus <t>faecalis</t> and pSM19035 from Streptococcus pyogenes , as indicated; (iv) numbering in plasmids indicates start and end positions of regions of homology to sequences in the contig; (v) purple bars, constituted by truncated insertion sequence (IS) elements, separate non-colinear regions of homology; (vi) blue bars indicate regions of low homology to those found in pRE25 and pSM19035; (vii) the broken line indicates sequences not present in pSM19035.
    E Faecalis, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 92/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC e faecalis atcc 51299
    PCR-HRMA amplification curves of vancomycin-resistant <t>(VRE)</t> and -sensitive (VSE) E <t>faecalis</t> and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis <t>ATCC</t> 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).
    E Faecalis Atcc 51299, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Symbiopharm e faecalis
    PCR-HRMA amplification curves of vancomycin-resistant <t>(VRE)</t> and -sensitive (VSE) E <t>faecalis</t> and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis <t>ATCC</t> 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).
    E Faecalis, supplied by Symbiopharm, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    99
    ATCC host e faecalis atcc 19433
    PCR-HRMA amplification curves of vancomycin-resistant <t>(VRE)</t> and -sensitive (VSE) E <t>faecalis</t> and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis <t>ATCC</t> 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).
    Host E Faecalis Atcc 19433, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Thermo Fisher custom e faecalis affymetrix genechip
    PCR-HRMA amplification curves of vancomycin-resistant <t>(VRE)</t> and -sensitive (VSE) E <t>faecalis</t> and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis <t>ATCC</t> 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).
    Custom E Faecalis Affymetrix Genechip, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    98
    ATCC enterococcus faecalis v583
    PCR-HRMA amplification curves of vancomycin-resistant <t>(VRE)</t> and -sensitive (VSE) E <t>faecalis</t> and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis <t>ATCC</t> 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).
    Enterococcus Faecalis V583, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 65 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SEM images of E. faecalis biofilm on the root canal walls and inside the dentinal tubules (a) ×2000 maginification, (b) ×3000 magnification, (c) ×5000 magnification, (d) ×8000 magnification

    Journal: Journal of Dentistry (Tehran, Iran)

    Article Title: Minimum Intracanal Dressing Time of Triple Antibiotic Paste to Eliminate Enterococcus Faecalis (ATCC 29212) and Determination of Minimum Inhibitory Concentration and Minimum Bactericidal Concentration: An Ex Vivo Study

    doi:

    Figure Lengend Snippet: SEM images of E. faecalis biofilm on the root canal walls and inside the dentinal tubules (a) ×2000 maginification, (b) ×3000 magnification, (c) ×5000 magnification, (d) ×8000 magnification

    Article Snippet: For this purpose, first 0.5 McFarland standard suspension (containing 1.5×106 CFUs/mL) of E. faecalis (ATCC 29212) was prepared using TSB culture medium and then the following steps were performed: Preparing 1 mg/mL concentration of TAP in cation-adjusted Mueller Hinton broth medium Transferring 50 μL of cation-adjusted Mueller Hinton broth medium to wells #2 to #11 of a microplate under sterile conditions (well #11 served as the negative control) Adding 100 μL of the TAP solution with 1 mg/mL concentration to well #1.

    Techniques:

    Surface hydrophobicity * of the wild type (WT) ( a) Listeria monocytogenes ATCC 53135, ( b ) L. monocytogenes MTCC 657 ( c ) E. faecium DSMZ 20477 , ( d ) E. faecium VRE, ( e ) E. faecalis ATCC 29212 and their nisin resistant (Nr), Pediocin 34 resistant (Pr) and Enterocin FH99 resistant (Er) variants. *Values are presented as mean±SEM; n = 3. ¥, ψ Values with different superscripts differ significantly at the level of p

    Journal: Journal of Food Science and Technology

    Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

    doi: 10.1007/s13197-011-0500-3

    Figure Lengend Snippet: Surface hydrophobicity * of the wild type (WT) ( a) Listeria monocytogenes ATCC 53135, ( b ) L. monocytogenes MTCC 657 ( c ) E. faecium DSMZ 20477 , ( d ) E. faecium VRE, ( e ) E. faecalis ATCC 29212 and their nisin resistant (Nr), Pediocin 34 resistant (Pr) and Enterocin FH99 resistant (Er) variants. *Values are presented as mean±SEM; n = 3. ¥, ψ Values with different superscripts differ significantly at the level of p

    Article Snippet: The nisin resistance phenotype in L.monocytogenes MTCC 657, E. faecium DSMZ 20477, E. faecium VRE and E.faecalis ATCC 29212 was stable during at 50, 40, 20 and 30 successive cultures, respectively, without nisin.

    Techniques:

    Representative colonial pictures of the materials against planktonic E.faecalis . Notes: ( A ) negative control group; ( B ) CH group; ( C ) MCSNs group; ( D ) Ag-MCSNs group; ( E ) Ag/Zn(9:1)-MCSNs group; ( F ) Ag/Zn(1:1)-MCSNs group; ( G ) Ag/Zn(1:9)-MCSNs group; ( H ) Zn-MCSNs group. Abbreviations: CH, calcium hydroxide; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Journal: International Journal of Nanomedicine

    Article Title: The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles

    doi: 10.2147/IJN.S244686

    Figure Lengend Snippet: Representative colonial pictures of the materials against planktonic E.faecalis . Notes: ( A ) negative control group; ( B ) CH group; ( C ) MCSNs group; ( D ) Ag-MCSNs group; ( E ) Ag/Zn(9:1)-MCSNs group; ( F ) Ag/Zn(1:1)-MCSNs group; ( G ) Ag/Zn(1:9)-MCSNs group; ( H ) Zn-MCSNs group. Abbreviations: CH, calcium hydroxide; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Article Snippet: Antimicrobial Effects of the Nanoparticles E. faecalis (ATCC 29212, Manassas, VA, USA) suspension was diluted to 1×104 colony forming units (CFUs)/mL.

    Techniques: Negative Control

    Antibacterial effects of the materials against planktonic E. faecalis. Notes: Comparisons of CFUs count among the groups, including Negative, CH, MCSNs, Ag-MCSNs, Ag/Zn(9:1)-MCSNs, Ag/Zn(1:1)-MCSNs, Ag/Zn(1:9)-MCSNs and Zn/MCSNs. * P

    Journal: International Journal of Nanomedicine

    Article Title: The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles

    doi: 10.2147/IJN.S244686

    Figure Lengend Snippet: Antibacterial effects of the materials against planktonic E. faecalis. Notes: Comparisons of CFUs count among the groups, including Negative, CH, MCSNs, Ag-MCSNs, Ag/Zn(9:1)-MCSNs, Ag/Zn(1:1)-MCSNs, Ag/Zn(1:9)-MCSNs and Zn/MCSNs. * P

    Article Snippet: Antimicrobial Effects of the Nanoparticles E. faecalis (ATCC 29212, Manassas, VA, USA) suspension was diluted to 1×104 colony forming units (CFUs)/mL.

    Techniques:

    Representative FE-SEM images showing colonization of E. faecalis on the pretreated root canal walls (capital letters: ×3000 magnification; small letters: ×10,000 magnification). Notes: ( A and a ) Negative control group; ( B and b ) CH group; ( C and c ) MCSNs group; ( D and d ) Ag-MCSNs group; ( E and e ) Zn-MCSNs group; ( F and f ) Ag/Zn(1:9)-MCSNs group; ( G and g ) Ag/Zn(1:1)-MCSNs group; ( H and h ) Ag/Zn(9:1)-MCSNs group. Abbreviations: FE-SEM, field emission scanning electron microscopy; CH, calcium hydroxide; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Journal: International Journal of Nanomedicine

    Article Title: The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles

    doi: 10.2147/IJN.S244686

    Figure Lengend Snippet: Representative FE-SEM images showing colonization of E. faecalis on the pretreated root canal walls (capital letters: ×3000 magnification; small letters: ×10,000 magnification). Notes: ( A and a ) Negative control group; ( B and b ) CH group; ( C and c ) MCSNs group; ( D and d ) Ag-MCSNs group; ( E and e ) Zn-MCSNs group; ( F and f ) Ag/Zn(1:9)-MCSNs group; ( G and g ) Ag/Zn(1:1)-MCSNs group; ( H and h ) Ag/Zn(9:1)-MCSNs group. Abbreviations: FE-SEM, field emission scanning electron microscopy; CH, calcium hydroxide; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Article Snippet: Antimicrobial Effects of the Nanoparticles E. faecalis (ATCC 29212, Manassas, VA, USA) suspension was diluted to 1×104 colony forming units (CFUs)/mL.

    Techniques: Negative Control, Electron Microscopy

    Representative TEM images of endocytosis of nanoparticles by E. faecalis. Notes: ( A ) E. faecalis (×30,000); ( B – F ) E. faecalis cocultured with Ag/Zn(1:1)-MCSNs. ×30,000 ( B ); ×100,000 ( C and E ); ×200,000 ( D and F ). Abbreviations: TEM, transmission electron microscopy; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated mesoporous calcium-silicate nanoparticles.

    Journal: International Journal of Nanomedicine

    Article Title: The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles

    doi: 10.2147/IJN.S244686

    Figure Lengend Snippet: Representative TEM images of endocytosis of nanoparticles by E. faecalis. Notes: ( A ) E. faecalis (×30,000); ( B – F ) E. faecalis cocultured with Ag/Zn(1:1)-MCSNs. ×30,000 ( B ); ×100,000 ( C and E ); ×200,000 ( D and F ). Abbreviations: TEM, transmission electron microscopy; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated mesoporous calcium-silicate nanoparticles.

    Article Snippet: Antimicrobial Effects of the Nanoparticles E. faecalis (ATCC 29212, Manassas, VA, USA) suspension was diluted to 1×104 colony forming units (CFUs)/mL.

    Techniques: Transmission Electron Microscopy, Transmission Assay, Electron Microscopy

    Representative E. faecalis biofilms (4-week old) on dentin surface after medication were scanned by SEM or FE-SEM (capital letters: ×3000 magnification; small letters: ×10,000 magnification). Notes: ( A and a ) Negative control group; ( B and b ) CH group; ( C and c ) MTA group; ( D and d ) MCSNs group; ( E and e ) Ag-MCSNs group; ( F and f ) Zn-MCSNs group; ( G and g ) Ag/Zn(1:9)-MCSNs group; ( H and h ) Ag/Zn(1:1)-MCSNs group; ( I and i ) Ag/Zn(9:1)-MCSNs group. Abbreviations: SEM, scanning electron microscopy; FE-SEM, field emission scanning electron microscopy; CH, calcium hydroxide; MTA, mineral trioxide aggregate; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Journal: International Journal of Nanomedicine

    Article Title: The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles

    doi: 10.2147/IJN.S244686

    Figure Lengend Snippet: Representative E. faecalis biofilms (4-week old) on dentin surface after medication were scanned by SEM or FE-SEM (capital letters: ×3000 magnification; small letters: ×10,000 magnification). Notes: ( A and a ) Negative control group; ( B and b ) CH group; ( C and c ) MTA group; ( D and d ) MCSNs group; ( E and e ) Ag-MCSNs group; ( F and f ) Zn-MCSNs group; ( G and g ) Ag/Zn(1:9)-MCSNs group; ( H and h ) Ag/Zn(1:1)-MCSNs group; ( I and i ) Ag/Zn(9:1)-MCSNs group. Abbreviations: SEM, scanning electron microscopy; FE-SEM, field emission scanning electron microscopy; CH, calcium hydroxide; MTA, mineral trioxide aggregate; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Article Snippet: Antimicrobial Effects of the Nanoparticles E. faecalis (ATCC 29212, Manassas, VA, USA) suspension was diluted to 1×104 colony forming units (CFUs)/mL.

    Techniques: Negative Control, Electron Microscopy

    CLSM 3-dimensional reconstructions of E. faecalis biofilms (4-week old) after medication (green: live cells; red: dead cells). Notes: ( A ) Negative control group; ( B ) CH group; ( C ) MTA group; ( D ) MCSNs group; ( E ) Ag-MCSNs group; ( F ) Zn-MCSNs group; ( G ) Ag/Zn(1:9)-MCSNs group; ( H ) Ag/Zn(1:1)-MCSNs group; ( I ) Ag/Zn(9:1)-MCSNs group. Abbreviations: CLSM, confocal laser scanning microscope; CH, calcium hydroxide; MTA, mineral trioxide aggregate; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Journal: International Journal of Nanomedicine

    Article Title: The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles

    doi: 10.2147/IJN.S244686

    Figure Lengend Snippet: CLSM 3-dimensional reconstructions of E. faecalis biofilms (4-week old) after medication (green: live cells; red: dead cells). Notes: ( A ) Negative control group; ( B ) CH group; ( C ) MTA group; ( D ) MCSNs group; ( E ) Ag-MCSNs group; ( F ) Zn-MCSNs group; ( G ) Ag/Zn(1:9)-MCSNs group; ( H ) Ag/Zn(1:1)-MCSNs group; ( I ) Ag/Zn(9:1)-MCSNs group. Abbreviations: CLSM, confocal laser scanning microscope; CH, calcium hydroxide; MTA, mineral trioxide aggregate; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Article Snippet: Antimicrobial Effects of the Nanoparticles E. faecalis (ATCC 29212, Manassas, VA, USA) suspension was diluted to 1×104 colony forming units (CFUs)/mL.

    Techniques: Confocal Laser Scanning Microscopy, Negative Control, Laser-Scanning Microscopy

    E. faecalis adherence and colonization on the pretreated root canal walls observed by CLSM (green: live cells; red: dead cells). Notes: ( A ) Blank control group (no background fluorescence produced by dentin); ( B ) Negative group; ( C ) CH group; ( D ) MCSNs group; ( E ) Ag-MCSNs group; ( F ) Zn-MCSNs group; ( G ) Ag/Zn(1:9)-MCSNs group; ( H ) Ag/Zn(1:1)-MCSNs group; ( I ) Ag/Zn(9:1)-MCSNs group. Abbreviations: CLSM, confocal laser scanning microscope; CH, calcium hydroxide; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Journal: International Journal of Nanomedicine

    Article Title: The Antibiofilm Activity and Mechanism of Nanosilver- and Nanozinc-Incorporated Mesoporous Calcium-Silicate Nanoparticles

    doi: 10.2147/IJN.S244686

    Figure Lengend Snippet: E. faecalis adherence and colonization on the pretreated root canal walls observed by CLSM (green: live cells; red: dead cells). Notes: ( A ) Blank control group (no background fluorescence produced by dentin); ( B ) Negative group; ( C ) CH group; ( D ) MCSNs group; ( E ) Ag-MCSNs group; ( F ) Zn-MCSNs group; ( G ) Ag/Zn(1:9)-MCSNs group; ( H ) Ag/Zn(1:1)-MCSNs group; ( I ) Ag/Zn(9:1)-MCSNs group. Abbreviations: CLSM, confocal laser scanning microscope; CH, calcium hydroxide; MCSNs, mesoporous calcium-silicate nanoparticles; Ag-MCSNs, nanosilver-incorporated MCSNs; Ag/Zn-MCSNs, nanosilver- and nanozinc-incorporated MCSNs; Zn-MCSNs, nanozinc-incorporated MCSNs.

    Article Snippet: Antimicrobial Effects of the Nanoparticles E. faecalis (ATCC 29212, Manassas, VA, USA) suspension was diluted to 1×104 colony forming units (CFUs)/mL.

    Techniques: Confocal Laser Scanning Microscopy, Fluorescence, Produced, Laser-Scanning Microscopy

    Effects of root canal sealers on biofilms from two Enterococcus faecalis strains. Microtiter-plate crystal violet antibiofilm assay

    Journal: Contemporary Clinical Dentistry

    Article Title: Comparative Evaluation of Antibiofilm Efficacy of Chitosan Nanoparticle- and Zinc Oxide Nanoparticle-Incorporated Calcium Hydroxide-Based Sealer: An In vitro Study

    doi: 10.4103/ccd.ccd_225_18

    Figure Lengend Snippet: Effects of root canal sealers on biofilms from two Enterococcus faecalis strains. Microtiter-plate crystal violet antibiofilm assay

    Article Snippet: Two E. faecalis strains (ATCC 29212, OG1RF) were used for the study.

    Techniques: Antibiofilm Assay

    The three-dimensional CLSM reconstruction of Enterococcus faecalis strain ATCC 29212 (a) and OG1RF (b), after treatment with antibacterial zinc oxide nanoparticles against ATCC 29212 (c) and OG1RF (d), after treatment with chitosan nanoparticles against ATCC 29212 (e) and OG1RF (f)

    Journal: Contemporary Clinical Dentistry

    Article Title: Comparative Evaluation of Antibiofilm Efficacy of Chitosan Nanoparticle- and Zinc Oxide Nanoparticle-Incorporated Calcium Hydroxide-Based Sealer: An In vitro Study

    doi: 10.4103/ccd.ccd_225_18

    Figure Lengend Snippet: The three-dimensional CLSM reconstruction of Enterococcus faecalis strain ATCC 29212 (a) and OG1RF (b), after treatment with antibacterial zinc oxide nanoparticles against ATCC 29212 (c) and OG1RF (d), after treatment with chitosan nanoparticles against ATCC 29212 (e) and OG1RF (f)

    Article Snippet: Two E. faecalis strains (ATCC 29212, OG1RF) were used for the study.

    Techniques: Confocal Laser Scanning Microscopy

    Effects of sealers on bacterial cell viability and biofilm thickness from two Enterococcus faecalis strains. CLSM study

    Journal: Contemporary Clinical Dentistry

    Article Title: Comparative Evaluation of Antibiofilm Efficacy of Chitosan Nanoparticle- and Zinc Oxide Nanoparticle-Incorporated Calcium Hydroxide-Based Sealer: An In vitro Study

    doi: 10.4103/ccd.ccd_225_18

    Figure Lengend Snippet: Effects of sealers on bacterial cell viability and biofilm thickness from two Enterococcus faecalis strains. CLSM study

    Article Snippet: Two E. faecalis strains (ATCC 29212, OG1RF) were used for the study.

    Techniques: Confocal Laser Scanning Microscopy

    PCR screening for the presence of virulence genes in E. faecium FL31. Lanes 1, 3, 5, 7, and 9: amplification products of corresponding genes obtained from genomic DNA of the positive control E. faecalis ATCC 29212; lanes 2, 4, 6, 8, and 10: amplification products of corresponding genes obtained from genomic DNA of E. faecium FL31.

    Journal: BioMed Research International

    Article Title: Safety Aspect of Enterococcus faecium FL31 Strain and Antibacterial Mechanism of Its Hydroxylated Bacteriocin BacFL31 against Listeria monocytogenes

    doi: 10.1155/2018/5308464

    Figure Lengend Snippet: PCR screening for the presence of virulence genes in E. faecium FL31. Lanes 1, 3, 5, 7, and 9: amplification products of corresponding genes obtained from genomic DNA of the positive control E. faecalis ATCC 29212; lanes 2, 4, 6, 8, and 10: amplification products of corresponding genes obtained from genomic DNA of E. faecium FL31.

    Article Snippet: Amplification products of these virulent genes were observed only for the positive control strain E. faecalis ATCC 29212 ( ).

    Techniques: Polymerase Chain Reaction, Amplification, Positive Control

    In vitro analysis of Ef -OAD stability as a function of pH. E. faecalis cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4

    Journal: Applied and Environmental Microbiology

    Article Title: Biochemical and Genetic Characterization of the Enterococcus faecalis Oxaloacetate Decarboxylase Complex

    doi: 10.1128/AEM.03980-12

    Figure Lengend Snippet: In vitro analysis of Ef -OAD stability as a function of pH. E. faecalis cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4

    Article Snippet: Cultures of E. faecalis were grown at 37°C, without shaking, in Luria-Bertani medium (LB; Difco, NJ), initial pH 7.0, and supplemented with 33 mM trisodium citrate (LBC).

    Techniques: In Vitro, Incubation, Binding Assay

    Physiological characterization of oad mutants. (A) Growth curves of wild-type E. faecalis (●) and oadA (■), oadB (▼), and oadD (⧫) derivative strains in LBC. (B) Cytoplasmic pH variation (pH int ) of E. faecalis wild-type

    Journal: Applied and Environmental Microbiology

    Article Title: Biochemical and Genetic Characterization of the Enterococcus faecalis Oxaloacetate Decarboxylase Complex

    doi: 10.1128/AEM.03980-12

    Figure Lengend Snippet: Physiological characterization of oad mutants. (A) Growth curves of wild-type E. faecalis (●) and oadA (■), oadB (▼), and oadD (⧫) derivative strains in LBC. (B) Cytoplasmic pH variation (pH int ) of E. faecalis wild-type

    Article Snippet: Cultures of E. faecalis were grown at 37°C, without shaking, in Luria-Bertani medium (LB; Difco, NJ), initial pH 7.0, and supplemented with 33 mM trisodium citrate (LBC).

    Techniques:

    Effect of the deletion of the ef_3196/7 operon on virulence. Deletion mutant of the in vivo induced genes encoding a two-component system ( ef_3196/7 operon) has been constructed. Surviving G. mellonella larvae are counted from 16 to 24 hours after infection with E. faecalis V19 (black bar) and E. faecalis ΔTCS (white bar). Bars indicated by an asterisk show a significant difference in comparison with wild type (*, p≤0.05). Experiments were repeated at least three times.

    Journal: PLoS ONE

    Article Title: Screening of In Vivo Activated Genes in Enterococcus faecalis during Insect and Mouse Infections and Growth in Urine

    doi: 10.1371/journal.pone.0011879

    Figure Lengend Snippet: Effect of the deletion of the ef_3196/7 operon on virulence. Deletion mutant of the in vivo induced genes encoding a two-component system ( ef_3196/7 operon) has been constructed. Surviving G. mellonella larvae are counted from 16 to 24 hours after infection with E. faecalis V19 (black bar) and E. faecalis ΔTCS (white bar). Bars indicated by an asterisk show a significant difference in comparison with wild type (*, p≤0.05). Experiments were repeated at least three times.

    Article Snippet: E. coli and E. faecalis were transformed by electroporation with the Gene Pulser Apparatus (Bio-Rad), as described by Dower et al and Holo & Nes , respectively.

    Techniques: Mutagenesis, In Vivo, Construct, Infection

    R-IVET strategy employed in E. faecalis to identify promoters that are induced in vivo . A: Modification of Lox2 strain occurring when a promoter is active . Chromosome of V583 strain was modified by integrating ermB gene and a promoterless copy of tetM gene, first of which is flanked by two copies of site-specific recombination sequences ( loxP ). A V583 genomic library was constructed by cloning DNA fragments in plasmid pCre2 upstream from cre gene. If an active promoter is thus cloned, the recombinase Cre would be expressed resulting in a homologous recombination event between both loxP sites. This triggers the irreversible excision of the ermB gene and tetM becomes functional. B: In vivo screening of E. faecalis V583 genomic library . After elimination of cells containing an active promoter under in vitro conditions, the resulting library is administrated to the animal host model. After infection, changes in the antibiotic resistance phenotype allow to select resolved bacteria containing an in vivo induced promoter.

    Journal: PLoS ONE

    Article Title: Screening of In Vivo Activated Genes in Enterococcus faecalis during Insect and Mouse Infections and Growth in Urine

    doi: 10.1371/journal.pone.0011879

    Figure Lengend Snippet: R-IVET strategy employed in E. faecalis to identify promoters that are induced in vivo . A: Modification of Lox2 strain occurring when a promoter is active . Chromosome of V583 strain was modified by integrating ermB gene and a promoterless copy of tetM gene, first of which is flanked by two copies of site-specific recombination sequences ( loxP ). A V583 genomic library was constructed by cloning DNA fragments in plasmid pCre2 upstream from cre gene. If an active promoter is thus cloned, the recombinase Cre would be expressed resulting in a homologous recombination event between both loxP sites. This triggers the irreversible excision of the ermB gene and tetM becomes functional. B: In vivo screening of E. faecalis V583 genomic library . After elimination of cells containing an active promoter under in vitro conditions, the resulting library is administrated to the animal host model. After infection, changes in the antibiotic resistance phenotype allow to select resolved bacteria containing an in vivo induced promoter.

    Article Snippet: E. coli and E. faecalis were transformed by electroporation with the Gene Pulser Apparatus (Bio-Rad), as described by Dower et al and Holo & Nes , respectively.

    Techniques: In Vivo, Modification, Construct, Clone Assay, Plasmid Preparation, Homologous Recombination, Functional Assay, In Vitro, Infection

    Locations of in vivo induced promoters on E. faecalis chromosome. Promoters that were shown to be induced during persistence in Galleria mellonella larvae are located in open boxes and arrows below the boxes indicate the orientation in which promoters were cloned upstream from the recombinase gene in plasmid pCre2. Thick arrows represent Open Reading Frames located in the region neighboring these promoters. A : Pivi2 is located within a 1.2 kb-long region overlapping ef_0040 , ef_0041 and the untranslated region between them. Pivi2 probably regulates ef_0041 expression. B : Pivi3 is located within a 1.1 kb-long fragment overlapping ef_3282 and ef_3283 and should control ef_3282 expression. C : Pivi4 is included in a 550 pb-long region overlapping ef_3198 and a small part of untranslated region between ef_3197 and ef_3198 . This suggests that Pivi4 regulates the expression of the two-component system encoding operon ef_3196/7 . D : Pivi5 is located in a 1.2 kb-long fragment overlapping ef_0091 , ef_0092 and the untranslated region between them. Pivi5 could regulate ef_0092 and/or ef_0093 expression. E : Pivi6 is included in a 620 pb-long region overlapping ef_0376 and the untranslated fragment between this gene and ef_0377 . So Pivi6 could control expression of ef_0377 and ef_0378 which seem to be co-transcribed.

    Journal: PLoS ONE

    Article Title: Screening of In Vivo Activated Genes in Enterococcus faecalis during Insect and Mouse Infections and Growth in Urine

    doi: 10.1371/journal.pone.0011879

    Figure Lengend Snippet: Locations of in vivo induced promoters on E. faecalis chromosome. Promoters that were shown to be induced during persistence in Galleria mellonella larvae are located in open boxes and arrows below the boxes indicate the orientation in which promoters were cloned upstream from the recombinase gene in plasmid pCre2. Thick arrows represent Open Reading Frames located in the region neighboring these promoters. A : Pivi2 is located within a 1.2 kb-long region overlapping ef_0040 , ef_0041 and the untranslated region between them. Pivi2 probably regulates ef_0041 expression. B : Pivi3 is located within a 1.1 kb-long fragment overlapping ef_3282 and ef_3283 and should control ef_3282 expression. C : Pivi4 is included in a 550 pb-long region overlapping ef_3198 and a small part of untranslated region between ef_3197 and ef_3198 . This suggests that Pivi4 regulates the expression of the two-component system encoding operon ef_3196/7 . D : Pivi5 is located in a 1.2 kb-long fragment overlapping ef_0091 , ef_0092 and the untranslated region between them. Pivi5 could regulate ef_0092 and/or ef_0093 expression. E : Pivi6 is included in a 620 pb-long region overlapping ef_0376 and the untranslated fragment between this gene and ef_0377 . So Pivi6 could control expression of ef_0377 and ef_0378 which seem to be co-transcribed.

    Article Snippet: E. coli and E. faecalis were transformed by electroporation with the Gene Pulser Apparatus (Bio-Rad), as described by Dower et al and Holo & Nes , respectively.

    Techniques: In Vivo, Clone Assay, Plasmid Preparation, Expressing

    Effect of the deletion of the ef_0377 gene on virulence. Deletion mutant of the in vivo induced genes encoding an ankyrin repeat protein ( ef_0377 ) has been constructed. Surviving insects are counted from 16 to 24 hours after infection with E. faecalis V19 (black bar) and E. faecalis Δ377 (white bar). Bars indicated by an asterisk show a significant difference in comparison with wild type (*, p≤0.05; **, p≤0.01). Experiments were repeated at least three times.

    Journal: PLoS ONE

    Article Title: Screening of In Vivo Activated Genes in Enterococcus faecalis during Insect and Mouse Infections and Growth in Urine

    doi: 10.1371/journal.pone.0011879

    Figure Lengend Snippet: Effect of the deletion of the ef_0377 gene on virulence. Deletion mutant of the in vivo induced genes encoding an ankyrin repeat protein ( ef_0377 ) has been constructed. Surviving insects are counted from 16 to 24 hours after infection with E. faecalis V19 (black bar) and E. faecalis Δ377 (white bar). Bars indicated by an asterisk show a significant difference in comparison with wild type (*, p≤0.05; **, p≤0.01). Experiments were repeated at least three times.

    Article Snippet: E. coli and E. faecalis were transformed by electroporation with the Gene Pulser Apparatus (Bio-Rad), as described by Dower et al and Holo & Nes , respectively.

    Techniques: Mutagenesis, In Vivo, Construct, Infection

    Comparison of inhibition zones of control group A vs . Experimental groups B, C, and D. (A) For all tested organisms; (B) for E. faecalis ; (C) for S. aureus ; (D) for C. albicans. Group A (control), sodium hypochlorite; group B (OT), obicure tea extract; group C (LG), lemon grass oil; group D (BO), basil oil.

    Journal: Restorative Dentistry & Endodontics

    Article Title: Evaluation of antimicrobial activity and efficacy of herbal oils and extracts in disinfection of gutta percha cones before obturation

    doi: 10.5395/rde.2017.42.4.264

    Figure Lengend Snippet: Comparison of inhibition zones of control group A vs . Experimental groups B, C, and D. (A) For all tested organisms; (B) for E. faecalis ; (C) for S. aureus ; (D) for C. albicans. Group A (control), sodium hypochlorite; group B (OT), obicure tea extract; group C (LG), lemon grass oil; group D (BO), basil oil.

    Article Snippet: Evaluation of antimicrobial efficacy of herbal extracts The inoculums of S. aureus and E. faecalis were incubated overnight in nutrient broth (Hi-media Laboratories, Mumbai, MH, India) to collect sufficient number of microbial colonies.

    Techniques: Inhibition

    In vitro antibiofilm and antibacterial activity of Enterococcus faecalis : ( A ) BA and derivatives; ( B ) UA and derivatives. The results represent the mean ± standard deviation of five experiments. * Represents a significant difference in relation to the biofilm formation control ( p

    Journal: Biomolecules

    Article Title: Triterpene Derivatives as Relevant Scaffold for New Antibiofilm Drugs

    doi: 10.3390/biom9020058

    Figure Lengend Snippet: In vitro antibiofilm and antibacterial activity of Enterococcus faecalis : ( A ) BA and derivatives; ( B ) UA and derivatives. The results represent the mean ± standard deviation of five experiments. * Represents a significant difference in relation to the biofilm formation control ( p

    Article Snippet: Antibiofilm and Antibacterial Activity Assays A planktonic susceptibility testing of S. epidermidis, S. aureus and E. faecalis was performed by the reference broth microdilution assay according to Clinical and Laboratory Standards Institute (CLSI) guidelines [ ].

    Techniques: In Vitro, Activity Assay, Standard Deviation

    Genetic organization of ORFs in the contig harboring the tetracycline resistance gene tet (S) from the genome sequence of S. thermophilus St-9 (A) and in the contig harboring the erythromycin resistance gene ermB from the genome of S. thermophilus St-5 (B) . Color code of the ORFs: in yellow, ORFs complete and/or incomplete ORFs related to transposases, invertases, and topoisomerases; in purple, ORFs involved in antibiotic resistance; in light blue, ORFs related to plasmid segregation and stability; in green, ORF involved in conjugation/mobilization; in white, ORFs encoding hypothetical proteins or proteins coding for other systems. Specific features of (B) : (i) the position of oriT is also indicated; (ii) arrowheads represent a region of direct repeats (DR) in front of the replication module, which showed no significant homology to those in pRE25 and pSM19035; (iii) long segments of the contig (black bars) highly similar ( > 99% nucleotide identity) or identical to plasmid sequences of pRE25 from Enterococcus faecalis and pSM19035 from Streptococcus pyogenes , as indicated; (iv) numbering in plasmids indicates start and end positions of regions of homology to sequences in the contig; (v) purple bars, constituted by truncated insertion sequence (IS) elements, separate non-colinear regions of homology; (vi) blue bars indicate regions of low homology to those found in pRE25 and pSM19035; (vii) the broken line indicates sequences not present in pSM19035.

    Journal: Frontiers in Microbiology

    Article Title: Antibiotic Resistance-Susceptibility Profiles of Streptococcus thermophilus Isolated from Raw Milk and Genome Analysis of the Genetic Basis of Acquired Resistances

    doi: 10.3389/fmicb.2017.02608

    Figure Lengend Snippet: Genetic organization of ORFs in the contig harboring the tetracycline resistance gene tet (S) from the genome sequence of S. thermophilus St-9 (A) and in the contig harboring the erythromycin resistance gene ermB from the genome of S. thermophilus St-5 (B) . Color code of the ORFs: in yellow, ORFs complete and/or incomplete ORFs related to transposases, invertases, and topoisomerases; in purple, ORFs involved in antibiotic resistance; in light blue, ORFs related to plasmid segregation and stability; in green, ORF involved in conjugation/mobilization; in white, ORFs encoding hypothetical proteins or proteins coding for other systems. Specific features of (B) : (i) the position of oriT is also indicated; (ii) arrowheads represent a region of direct repeats (DR) in front of the replication module, which showed no significant homology to those in pRE25 and pSM19035; (iii) long segments of the contig (black bars) highly similar ( > 99% nucleotide identity) or identical to plasmid sequences of pRE25 from Enterococcus faecalis and pSM19035 from Streptococcus pyogenes , as indicated; (iv) numbering in plasmids indicates start and end positions of regions of homology to sequences in the contig; (v) purple bars, constituted by truncated insertion sequence (IS) elements, separate non-colinear regions of homology; (vi) blue bars indicate regions of low homology to those found in pRE25 and pSM19035; (vii) the broken line indicates sequences not present in pSM19035.

    Article Snippet: Unless otherwise stated, S. thermophilus strains were grown statically under anaerobic conditions in M17 (Oxoid, Basingstoke, UK) with 0.5% lactose (LM17) or Mueller-Hinton (Oxoid) at 37°C for 24–48 h. L. plantarum and E. faecalis were grown under aerobic conditions at 30°C in de Man Rogosa and Sharpe (MRS) broth (Merck, Darmstad, Germany).

    Techniques: Sequencing, Plasmid Preparation, Conjugation Assay

    PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA amplification curves of vancomycin-resistant (VRE) and -sensitive (VSE) E faecalis and E faecium . Amplification curves for VRE ( E faecium ATCC 19434, E faecalis ATCC 51299 and E faecalis ATCC 29212) and the VSE standard strains ( E faecalis NCTC 77 and E faecium NCIMB 2699). Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves. B fragilis amplifies with the same efficiency as the Enterococcus strains. Nuclease-free water was used as a negative control (NC).

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains (E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains (E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Polymerase Chain Reaction, Amplification, Negative Control

    Concentration dependence of RT-PCR-HRMA using the V1 primer set: Amplification curves for different quantities of DNA from VRE E faecalis ATCC 29212 (250 fg to 250 ng) using the V1 primer pair.

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: Concentration dependence of RT-PCR-HRMA using the V1 primer set: Amplification curves for different quantities of DNA from VRE E faecalis ATCC 29212 (250 fg to 250 ng) using the V1 primer pair.

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains (E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains (E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Concentration Assay, Reverse Transcription Polymerase Chain Reaction, Amplification

    Analytical sensitivity of VRE PCR-HRMA assay using the V1 primer set. Data show that the characteristic difference curve shape for VRE (using DNA from VRE E faecalis strain ATCC 29212) is maintained over a 6-log concentration range (250 fg to 250 ng DNA).

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: Analytical sensitivity of VRE PCR-HRMA assay using the V1 primer set. Data show that the characteristic difference curve shape for VRE (using DNA from VRE E faecalis strain ATCC 29212) is maintained over a 6-log concentration range (250 fg to 250 ng DNA).

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains (E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains (E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Polymerase Chain Reaction, Concentration Assay

    PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Journal: Annals of Saudi Medicine

    Article Title: A novel high-resolution melting analysis approach for rapid detection of vancomycin-resistant enterococci

    doi: 10.5144/0256-4947.2018.200

    Figure Lengend Snippet: PCR-HRMA difference plots of VRE and VSE E faecalis and E faecium generated against B fragilis using V1 primer set. Difference curve plots for (A) VSE (NCTC 77, NCIMB 2699) and (B) vanA/B VRE (ATCC 19434, ATCC 51299, ATCC 29212) standard strains of E faecalis and E faecium . Each assay contained 250 pg of bacterial DNA. B fragilis was also included as the reference organism for generating VRE/VSE difference curves (plots). B fragilis amplifies with the same efficiency as the E faecalis and E faecium strains.

    Article Snippet: This study was conducted on five reference strains that belong to two groups: vancomycin-resistant E faecalis and E faecium strains (E faecium ATCC 19434 (VRE), E faecalis ATCC 29212 (VRE) and E faecalis ATCC 51299 (VRE)), and vancomycin-sensitive E faecalis and E faecium strains (E faecalis NCTC 77 (VSE), E faecium NCIMB 2699 (VSE)).

    Techniques: Polymerase Chain Reaction, Generated

    Time-killkinetics of uperin 3.6 against the following quality control strains: MS S. aureus ATCC 29213, MR S. aureus ATCC 43300, VS E. faecalis ATCC 29212, VR E. faecalis ATCC 51299, R. equi ATCC 6939, and S. pyogenes ATCC 19615.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: In Vitro Activity and Killing Effect of Uperin 3.6 against Gram-Positive Cocci Isolated from Immunocompromised Patients

    doi: 10.1128/AAC.49.9.3933-3936.2005

    Figure Lengend Snippet: Time-killkinetics of uperin 3.6 against the following quality control strains: MS S. aureus ATCC 29213, MR S. aureus ATCC 43300, VS E. faecalis ATCC 29212, VR E. faecalis ATCC 51299, R. equi ATCC 6939, and S. pyogenes ATCC 19615.

    Article Snippet: The quality control strains included methicillin-susceptible (MS) Staphylococcus aureus ATCC 29213, MR S. aureus ATCC 43300, vancomycin-susceptible (VS) Enterococcus faecalis ATCC 29212, VR E. faecalis ATCC 51299, R. equi ATCC 6939, and Streptococcus pyogenes ATCC 19615.

    Techniques: Mass Spectrometry