e. faecalis Search Results


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  • 99
    ATCC e faecalis
    SEM images of E. <t>faecalis</t> biofilm on the root canal walls and inside the dentinal tubules (a) ×2000 maginification, (b) ×3000 magnification, (c) ×5000 magnification, (d) ×8000 magnification
    E Faecalis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2170 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore polyclonal rabbit anti e faecalis antibodies
    SEM images of E. <t>faecalis</t> biofilm on the root canal walls and inside the dentinal tubules (a) ×2000 maginification, (b) ×3000 magnification, (c) ×5000 magnification, (d) ×8000 magnification
    Polyclonal Rabbit Anti E Faecalis Antibodies, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories e faecalis
    Comparison of inhibition zones of control group A vs . Experimental groups B, C, and D. (A) For all tested organisms; (B) for E. <t>faecalis</t> ; (C) for S. aureus ; (D) for C. albicans. Group A (control), sodium hypochlorite; group B (OT), obicure tea extract; group C (LG), lemon grass oil; group D (BO), basil oil.
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    Difco e faecalis
    In vitro analysis of Ef -OAD stability as a function of pH. E. <t>faecalis</t> cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4
    E Faecalis, supplied by Difco, used in various techniques. Bioz Stars score: 93/100, based on 106 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Symbiopharm e faecalis
    In vitro analysis of Ef -OAD stability as a function of pH. E. <t>faecalis</t> cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4
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    Bio-Rad e faecalis
    Drosophila -health by E. <t>faecalis</t> -load curve. Source data used to construct this figure was obtained from results on Figure 2 , only considering time points at which enough flies alive were available. All strains show two different slopes corresponding to different tolerance values, revealing that at some point (pathogen load value) there is a huge decrease in tolerance to E. faecalis . This inflection point corresponds to a lower pathogen load for the wild type strain (10 5 ), when compared to the mutant strains (10 6 ). For 10 6 value of pathogen load, the wild type induced only 10% survival in the Drosophila population, as opposed to 90% survival of the Drosophila population infected with the triple mutant.
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    93
    Merck KGaA e faecalis
    Genetic organization of ORFs in the contig harboring the tetracycline resistance gene tet (S) from the genome sequence of S. thermophilus St-9 (A) and in the contig harboring the erythromycin resistance gene ermB from the genome of S. thermophilus St-5 (B) . Color code of the ORFs: in yellow, ORFs complete and/or incomplete ORFs related to transposases, invertases, and topoisomerases; in purple, ORFs involved in antibiotic resistance; in light blue, ORFs related to plasmid segregation and stability; in green, ORF involved in conjugation/mobilization; in white, ORFs encoding hypothetical proteins or proteins coding for other systems. Specific features of (B) : (i) the position of oriT is also indicated; (ii) arrowheads represent a region of direct repeats (DR) in front of the replication module, which showed no significant homology to those in pRE25 and pSM19035; (iii) long segments of the contig (black bars) highly similar ( > 99% nucleotide identity) or identical to plasmid sequences of pRE25 from Enterococcus <t>faecalis</t> and pSM19035 from Streptococcus pyogenes , as indicated; (iv) numbering in plasmids indicates start and end positions of regions of homology to sequences in the contig; (v) purple bars, constituted by truncated insertion sequence (IS) elements, separate non-colinear regions of homology; (vi) blue bars indicate regions of low homology to those found in pRE25 and pSM19035; (vii) the broken line indicates sequences not present in pSM19035.
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    Clinical and Laboratory Standards Institute e faecalis
    In vitro antibiofilm and antibacterial activity of Enterococcus <t>faecalis</t> : ( A ) BA and derivatives; ( B ) UA and derivatives. The results represent the mean ± standard deviation of five experiments. * Represents a significant difference in relation to the biofilm formation control ( p
    E Faecalis, supplied by Clinical and Laboratory Standards Institute, used in various techniques. Bioz Stars score: 94/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Inserm Transfert e faecalis
    Psh is activated by bacterial proteolytic activities in the hemolymph Toll pathway activation was monitored in adult flies by RNA hybridization analysis of drs messenger expression. Rp49 messenger was used as loading control and for normalization of drs expression. drs mRNA abundance ( drs/Rp49 ) in wild-type ( WT ) flies was set to 100 as a control and values obtained with mutant flies were expressed as percentage of this value. This result is representative of 3 independent experiments and histograms represent the mean of these results. Error bars are SD. ( a,b ) drs expression 24 hours after injection of E. <t>faecalis</t> peptidoglycan is not significantly affected in psh mutant flies ( P = 0.24) but is reduced in grass Herrade ( hrd ) ( P = 2.3 × 10 -6 ) or psh , hrd double mutant flies ( P = 0.009) at a very comparable level ( P = 0.45). ( c,d ) drs expression 24 hours after injection of heat-killed E. faecalis is not significantly affected in psh mutant flies ( P = 0.05) but is highly reduced in hrd single ( P = 1.6 × 10 -5 ) or psh, hrd double mutant flies ( P = 6 × 10 -5 ) at a very comparable level ( P = 0.45). ( e ) drs expression 24 hours after infection of WT flies with living or heat-killed E. faecalis is significantly reduced in flies challenged with dead bacteria ( P = 2.7 × 10 -5 ). ( f,g ) drs expression 24 hours after injection of bacterial subtilisin is not affected in hrd mutant ( P = 0.8) but is highly reduced in psh mutant flies ( P = 1,9 × 10 -8 ). The reduction in drs expression observed in psh is identical to that observed in the hemizygous SPE Pasteur mutant ( pstr /Df1) ( P = 0.58) and is not further enhanced in psh , hrd double mutant flies ( P = 0.1).
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    96
    Millipore e faecalis
    The ebp operon in E. <t>faecalis</t> OG1RF encodes the Ebp pilus structural subunits EbpA (blue), EbpB (purple), and EbpC (orange) and the pilus-associated sortase SrtC (green). The housekeeping sortase SrtA (brown) is encoded elsewhere in the genome (A). Sortase recognition motif amino acid sequences (LPETG for EbpA, LPKTN for EbpB, and LPSTG for EbpC), pilin motif Lys residues (K), and the sortase catalytic Cys residues (C) are shown by the one-letter code on their respective subunits. Predicted Srt subunit thioacyl intermediates are faded (A to E). EbpA incorporation relies on cleavage of its sortase recognition motif by SrtC and resolution of the resultant intermediate by the EbpC pilin motif Lys residue (B). EbpC polymerization requires SrtC transpeptidation between the sortase recognition motif of the most recently incorporated EbpC subunit of a growing fiber and the pilin motif Lys residue of an incoming EbpC subunit (C). EbpB is incorporated into a growing fiber via its pilin motif Lys residue (D). SrtA participates in anchoring fully polymerized pilus fibers to the cell wall, likely via transpeptidation of the EbpB sortase recognition motif with a cell wall precursor molecule.
    E Faecalis, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 123 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    86
    DSMZ e faecalis
    Time-course profile of growth, glucose consumption and lactate production by E . <t>faecalis</t> CBRD01 under anaerobic batch fermentation.A. Glucose consumption, biomass and lactate production.B. Specific rates of glucose uptake and lactate production.C. Volumetric productivity of lactate.
    E Faecalis, supplied by DSMZ, used in various techniques. Bioz Stars score: 86/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Bruker Corporation e faecalis
    Time-course profile of growth, glucose consumption and lactate production by E . <t>faecalis</t> CBRD01 under anaerobic batch fermentation.A. Glucose consumption, biomass and lactate production.B. Specific rates of glucose uptake and lactate production.C. Volumetric productivity of lactate.
    E Faecalis, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 94/100, based on 17 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    AnaSpec e faecalis
    (A and B) MCs derived from wild-type TLR2 −/− or MyD88 −/− mice were impaired in their capacity to degranulate (A) and to inhibit growth of E. <t>faecalis</t> (120 min of infection) (B). Each bar represents the mean ± SD
    E Faecalis, supplied by AnaSpec, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Olympus e faecalis suspension
    Schematic diagram of root canal sample preparation. (A) Single rooted teeth; the area from which the dentin block is prepared marked with red. (B) Standardized 4 mm root dentin block. (C) Standardized root canal diameter preparation with size 6 Gates Glidden (1.5 mm diameter). (D) The vertical direction of block splitting into to semi cylindrical halves. (E) The outer surfaces of the semicylindrical halves (the cemental side) were ground to achieve a standard thickness of 2 mm and to remove the root surface cement. (F) The specimen was inoculated with an overnight suspension of E. <t>faecalis</t> in BHI (2 ml) adjusted spectrophotometrically to OD 600 of 0.5, which was grown for 3 weeks. (G) Disinfecting the sample with different control and test irrigants. (H) Splitting the canal surface to label freshly exposed DT with LIVE/DEAD BacLight staining and examine them under CLSM.
    E Faecalis Suspension, supplied by Olympus, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    91
    Collaborative Drug Discovery Inc e faecalis assays
    LAMP assays of 10 5 E. <t>faecalis</t> non-lysed cells (with and without PMA treatment) and PMA treated lysed cells on a commercial PCR instrument.
    E Faecalis Assays, supplied by Collaborative Drug Discovery Inc, used in various techniques. Bioz Stars score: 91/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Solexa e faecalis strains
    Phylogenetic relationship among selected E. <t>faecalis</t> strains based on whole genome alignments. The alignment was calculated with Mugsy ( http://mugsy.sourceforge.net/ [last access 16.07.2014] [ 57 ]) and only aligned regions present in all analyzed strains were extracted. These regions were concatenated and positions with gaps removed. The resulting core alignment was used to infer a Maximum Likelihood tree with RAxML. The GTRGAMMA model for nucleotide substitution and rate heterogeneity was utilized, bootstrap support values of 1000 replicates are shown at the nodes. Names of the ST40 isolates and their origin are indicated at the end of the branches. The highly related ST40 isolates were further zoomed in exemplified by the dotted line and the different scale bar. Metadata are given as follows: Strain no., year of isolation, origin, country: AC, animal colonizer; AI, animal infection; HC, human colonizer; HI, human infection; CU, Cuba; D, Germany; DK, Denmark; ES, Spain; IS, Island; PL, Poland; USA.
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    94
    Clinical and Laboratory Standards Institute effect against e faecalis
    Phylogenetic relationship among selected E. <t>faecalis</t> strains based on whole genome alignments. The alignment was calculated with Mugsy ( http://mugsy.sourceforge.net/ [last access 16.07.2014] [ 57 ]) and only aligned regions present in all analyzed strains were extracted. These regions were concatenated and positions with gaps removed. The resulting core alignment was used to infer a Maximum Likelihood tree with RAxML. The GTRGAMMA model for nucleotide substitution and rate heterogeneity was utilized, bootstrap support values of 1000 replicates are shown at the nodes. Names of the ST40 isolates and their origin are indicated at the end of the branches. The highly related ST40 isolates were further zoomed in exemplified by the dotted line and the different scale bar. Metadata are given as follows: Strain no., year of isolation, origin, country: AC, animal colonizer; AI, animal infection; HC, human colonizer; HI, human infection; CU, Cuba; D, Germany; DK, Denmark; ES, Spain; IS, Island; PL, Poland; USA.
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    Covance e faecalis fusion protein
    E. <t>faecalis</t> ).
    E Faecalis Fusion Protein, supplied by Covance, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Biopure Corporation mtad against e faecalis
    E. <t>faecalis</t> ).
    Mtad Against E Faecalis, supplied by Biopure Corporation, used in various techniques. Bioz Stars score: 91/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher e faecalis hrm r
    E. <t>faecalis</t> ).
    E Faecalis Hrm R, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC pure e faecalis
    E. <t>faecalis</t> ).
    Pure E Faecalis, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Thermo Fisher e faecalis specific medium
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
    E Faecalis Specific Medium, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Germfree Laboratories Inc acute e faecalis infection
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
    Acute E Faecalis Infection, supplied by Germfree Laboratories Inc, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    HiMedia Laboratories e faecalis atcc29212 spores
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
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    ATCC 108 e faecalis
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
    108 E Faecalis, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Cytoskeleton Inc e faecalis ftsz proteins
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
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    Symbiopharm probiotic strain e faecalis symbioflor
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
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    ATCC activity against e faecalis
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
    Activity Against E Faecalis, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 22 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher custom e faecalis affymetrix genechip
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
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    SymBio e faecalis symbio bacterial lysate
    Agar experimentation showing that all specimens were contaminated by E. <t>Faecalis</t> .
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    Image Search Results


    SEM images of E. faecalis biofilm on the root canal walls and inside the dentinal tubules (a) ×2000 maginification, (b) ×3000 magnification, (c) ×5000 magnification, (d) ×8000 magnification

    Journal: Journal of Dentistry (Tehran, Iran)

    Article Title: Minimum Intracanal Dressing Time of Triple Antibiotic Paste to Eliminate Enterococcus Faecalis (ATCC 29212) and Determination of Minimum Inhibitory Concentration and Minimum Bactericidal Concentration: An Ex Vivo Study

    doi:

    Figure Lengend Snippet: SEM images of E. faecalis biofilm on the root canal walls and inside the dentinal tubules (a) ×2000 maginification, (b) ×3000 magnification, (c) ×5000 magnification, (d) ×8000 magnification

    Article Snippet: RESULTS and show the E. faecalis (ATCC 29212) colony count in the TAP group and the positive control group.

    Techniques:

    Effects of root canal sealers on biofilms from two Enterococcus faecalis strains. Microtiter-plate crystal violet antibiofilm assay

    Journal: Contemporary Clinical Dentistry

    Article Title: Comparative Evaluation of Antibiofilm Efficacy of Chitosan Nanoparticle- and Zinc Oxide Nanoparticle-Incorporated Calcium Hydroxide-Based Sealer: An In vitro Study

    doi: 10.4103/ccd.ccd_225_18

    Figure Lengend Snippet: Effects of root canal sealers on biofilms from two Enterococcus faecalis strains. Microtiter-plate crystal violet antibiofilm assay

    Article Snippet: Two E. faecalis strains (ATCC 29212, OG1RF) were used for the study.

    Techniques: Antibiofilm Assay

    The three-dimensional CLSM reconstruction of Enterococcus faecalis strain ATCC 29212 (a) and OG1RF (b), after treatment with antibacterial zinc oxide nanoparticles against ATCC 29212 (c) and OG1RF (d), after treatment with chitosan nanoparticles against ATCC 29212 (e) and OG1RF (f)

    Journal: Contemporary Clinical Dentistry

    Article Title: Comparative Evaluation of Antibiofilm Efficacy of Chitosan Nanoparticle- and Zinc Oxide Nanoparticle-Incorporated Calcium Hydroxide-Based Sealer: An In vitro Study

    doi: 10.4103/ccd.ccd_225_18

    Figure Lengend Snippet: The three-dimensional CLSM reconstruction of Enterococcus faecalis strain ATCC 29212 (a) and OG1RF (b), after treatment with antibacterial zinc oxide nanoparticles against ATCC 29212 (c) and OG1RF (d), after treatment with chitosan nanoparticles against ATCC 29212 (e) and OG1RF (f)

    Article Snippet: Two E. faecalis strains (ATCC 29212, OG1RF) were used for the study.

    Techniques: Confocal Laser Scanning Microscopy

    Effects of sealers on bacterial cell viability and biofilm thickness from two Enterococcus faecalis strains. CLSM study

    Journal: Contemporary Clinical Dentistry

    Article Title: Comparative Evaluation of Antibiofilm Efficacy of Chitosan Nanoparticle- and Zinc Oxide Nanoparticle-Incorporated Calcium Hydroxide-Based Sealer: An In vitro Study

    doi: 10.4103/ccd.ccd_225_18

    Figure Lengend Snippet: Effects of sealers on bacterial cell viability and biofilm thickness from two Enterococcus faecalis strains. CLSM study

    Article Snippet: Two E. faecalis strains (ATCC 29212, OG1RF) were used for the study.

    Techniques: Confocal Laser Scanning Microscopy

    Bacterial-stimulation index (bacteria-induced cytokine secretion) by macrophages from Crohn's disease (CD) and CD- infliximab (IFX) patients after infection with Escherichia coli (EC), E. faecalis (EC), Mycobacterium avium subsp. paratuberculosis (MAP)

    Journal: Clinical and Experimental Immunology

    Article Title: Infliximab therapy increases the frequency of circulating CD16+ monocytes and modifies macrophage cytokine response to bacterial infection

    doi: 10.1111/cei.12375

    Figure Lengend Snippet: Bacterial-stimulation index (bacteria-induced cytokine secretion) by macrophages from Crohn's disease (CD) and CD- infliximab (IFX) patients after infection with Escherichia coli (EC), E. faecalis (EC), Mycobacterium avium subsp. paratuberculosis (MAP)

    Article Snippet: Macrophage cultures were either left uninfected or were infected with M. avium subsp. paratuberculosis (MAP) (ATCC 43015, an isolate from a Crohn's patient), M. avium subsp. avium (MA) (strain 101, an isolate from an AIDS patient), E. coli (EC) (ATCC 25922) or E. faecalis (EC) (ATCC 29212) at a multiplicity of infection (MOI) of 10 bacteria : 1 cell.

    Techniques: Infection

    UPGMA dendrogram derived from RAPD ‐ PCR ‐fingerprinting patterns of all the 35 isolates using the primer 1254. Code and source of the isolates are indicated on the right‐hand side of the figure. The vertical dotted line indicates the 60% similarity level that delineates the species E. mundtii (cluster I), E. faecalis (cluster II ) and E. faecium (cluster III ). Isolates marked with * were identified by phenylalanyl‐ tRNA synthase α‐subunit ( pheS ) gene sequence analysis. G: maize grain; GS: maize grain silage; LS: lucerne silage; M: whole crop maize; MS: whole crop maize silage; R: ryegrass; RS: ryegrass silage; SC: starter cultures for silage.

    Journal: Microbial Biotechnology

    Article Title: Tyrosine decarboxylase activity of Enterococcus mundtii: new insights into phenotypic and genetic aspects

    doi: 10.1111/1751-7915.12402

    Figure Lengend Snippet: UPGMA dendrogram derived from RAPD ‐ PCR ‐fingerprinting patterns of all the 35 isolates using the primer 1254. Code and source of the isolates are indicated on the right‐hand side of the figure. The vertical dotted line indicates the 60% similarity level that delineates the species E. mundtii (cluster I), E. faecalis (cluster II ) and E. faecium (cluster III ). Isolates marked with * were identified by phenylalanyl‐ tRNA synthase α‐subunit ( pheS ) gene sequence analysis. G: maize grain; GS: maize grain silage; LS: lucerne silage; M: whole crop maize; MS: whole crop maize silage; R: ryegrass; RS: ryegrass silage; SC: starter cultures for silage.

    Article Snippet: On the contrary, lower sequence identity (76%) was achieved for strains belonging to the species E. faecalis (e.g. ATCC 29212 and V583).

    Techniques: Derivative Assay, Polymerase Chain Reaction, Sequencing, Mass Spectrometry

    Surface hydrophobicity * of the wild type (WT) ( a) Listeria monocytogenes ATCC 53135, ( b ) L. monocytogenes MTCC 657 ( c ) E. faecium DSMZ 20477 , ( d ) E. faecium VRE, ( e ) E. faecalis ATCC 29212 and their nisin resistant (Nr), Pediocin 34 resistant (Pr) and Enterocin FH99 resistant (Er) variants. *Values are presented as mean±SEM; n = 3. ¥, ψ Values with different superscripts differ significantly at the level of p

    Journal: Journal of Food Science and Technology

    Article Title: Antibacterial efficacy of nisin, pediocin 34 and enterocin FH99 against L. monocytogenes, E. faecium and E. faecalis and bacteriocin cross resistance and antibiotic susceptibility of their bacteriocin resistant variants

    doi: 10.1007/s13197-011-0500-3

    Figure Lengend Snippet: Surface hydrophobicity * of the wild type (WT) ( a) Listeria monocytogenes ATCC 53135, ( b ) L. monocytogenes MTCC 657 ( c ) E. faecium DSMZ 20477 , ( d ) E. faecium VRE, ( e ) E. faecalis ATCC 29212 and their nisin resistant (Nr), Pediocin 34 resistant (Pr) and Enterocin FH99 resistant (Er) variants. *Values are presented as mean±SEM; n = 3. ¥, ψ Values with different superscripts differ significantly at the level of p

    Article Snippet: The nisin resistance phenotype in L.monocytogenes MTCC 657, E. faecium DSMZ 20477, E. faecium VRE and E.faecalis ATCC 29212 was stable during at 50, 40, 20 and 30 successive cultures, respectively, without nisin.

    Techniques:

    Comparison of inhibition zones of control group A vs . Experimental groups B, C, and D. (A) For all tested organisms; (B) for E. faecalis ; (C) for S. aureus ; (D) for C. albicans. Group A (control), sodium hypochlorite; group B (OT), obicure tea extract; group C (LG), lemon grass oil; group D (BO), basil oil.

    Journal: Restorative Dentistry & Endodontics

    Article Title: Evaluation of antimicrobial activity and efficacy of herbal oils and extracts in disinfection of gutta percha cones before obturation

    doi: 10.5395/rde.2017.42.4.264

    Figure Lengend Snippet: Comparison of inhibition zones of control group A vs . Experimental groups B, C, and D. (A) For all tested organisms; (B) for E. faecalis ; (C) for S. aureus ; (D) for C. albicans. Group A (control), sodium hypochlorite; group B (OT), obicure tea extract; group C (LG), lemon grass oil; group D (BO), basil oil.

    Article Snippet: Evaluation of antimicrobial efficacy of herbal extracts The inoculums of S. aureus and E. faecalis were incubated overnight in nutrient broth (Hi-media Laboratories, Mumbai, MH, India) to collect sufficient number of microbial colonies.

    Techniques: Inhibition

    In vitro analysis of Ef -OAD stability as a function of pH. E. faecalis cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4

    Journal: Applied and Environmental Microbiology

    Article Title: Biochemical and Genetic Characterization of the Enterococcus faecalis Oxaloacetate Decarboxylase Complex

    doi: 10.1128/AEM.03980-12

    Figure Lengend Snippet: In vitro analysis of Ef -OAD stability as a function of pH. E. faecalis cell extracts were first incubated in batch with a neutravidin resin. After the binding step, the resin was washed (W; lane 3) with 1 ml 150 mM citrate, pH 7.0, and eluted (lanes 4

    Article Snippet: Cultures of E. faecalis were grown at 37°C, without shaking, in Luria-Bertani medium (LB; Difco, NJ), initial pH 7.0, and supplemented with 33 mM trisodium citrate (LBC).

    Techniques: In Vitro, Incubation, Binding Assay

    Physiological characterization of oad mutants. (A) Growth curves of wild-type E. faecalis (●) and oadA (■), oadB (▼), and oadD (⧫) derivative strains in LBC. (B) Cytoplasmic pH variation (pH int ) of E. faecalis wild-type

    Journal: Applied and Environmental Microbiology

    Article Title: Biochemical and Genetic Characterization of the Enterococcus faecalis Oxaloacetate Decarboxylase Complex

    doi: 10.1128/AEM.03980-12

    Figure Lengend Snippet: Physiological characterization of oad mutants. (A) Growth curves of wild-type E. faecalis (●) and oadA (■), oadB (▼), and oadD (⧫) derivative strains in LBC. (B) Cytoplasmic pH variation (pH int ) of E. faecalis wild-type

    Article Snippet: Cultures of E. faecalis were grown at 37°C, without shaking, in Luria-Bertani medium (LB; Difco, NJ), initial pH 7.0, and supplemented with 33 mM trisodium citrate (LBC).

    Techniques:

    Drosophila -health by E. faecalis -load curve. Source data used to construct this figure was obtained from results on Figure 2 , only considering time points at which enough flies alive were available. All strains show two different slopes corresponding to different tolerance values, revealing that at some point (pathogen load value) there is a huge decrease in tolerance to E. faecalis . This inflection point corresponds to a lower pathogen load for the wild type strain (10 5 ), when compared to the mutant strains (10 6 ). For 10 6 value of pathogen load, the wild type induced only 10% survival in the Drosophila population, as opposed to 90% survival of the Drosophila population infected with the triple mutant.

    Journal: PLoS ONE

    Article Title: Drosophila Host Model Reveals New Enterococcus faecalis Quorum-Sensing Associated Virulence Factors

    doi: 10.1371/journal.pone.0064740

    Figure Lengend Snippet: Drosophila -health by E. faecalis -load curve. Source data used to construct this figure was obtained from results on Figure 2 , only considering time points at which enough flies alive were available. All strains show two different slopes corresponding to different tolerance values, revealing that at some point (pathogen load value) there is a huge decrease in tolerance to E. faecalis . This inflection point corresponds to a lower pathogen load for the wild type strain (10 5 ), when compared to the mutant strains (10 6 ). For 10 6 value of pathogen load, the wild type induced only 10% survival in the Drosophila population, as opposed to 90% survival of the Drosophila population infected with the triple mutant.

    Article Snippet: Electrotransformation of E. coli and E. faecalis was carried out as described by Dower et al . (1988) and Dunny et al. (1991), using a Gene Pulser apparatus (Bio-Rad) , .

    Techniques: Construct, Mutagenesis, Infection

    Drosophila survival rates upon infection with E. faecalis strains. 75 Oregon R (5- to 7-day-old) male adult flies, raised at 25°C, were divided in tubes of 25 flies each, and infected, by septic injury onto the thorax with a thin needle, with V583 (A, B, D) and VE14089 derived strains (C). Data is representative of three independent experiments (225 flies per strain). Curves assigned with an * are significantly different (p

    Journal: PLoS ONE

    Article Title: Drosophila Host Model Reveals New Enterococcus faecalis Quorum-Sensing Associated Virulence Factors

    doi: 10.1371/journal.pone.0064740

    Figure Lengend Snippet: Drosophila survival rates upon infection with E. faecalis strains. 75 Oregon R (5- to 7-day-old) male adult flies, raised at 25°C, were divided in tubes of 25 flies each, and infected, by septic injury onto the thorax with a thin needle, with V583 (A, B, D) and VE14089 derived strains (C). Data is representative of three independent experiments (225 flies per strain). Curves assigned with an * are significantly different (p

    Article Snippet: Electrotransformation of E. coli and E. faecalis was carried out as described by Dower et al . (1988) and Dunny et al. (1991), using a Gene Pulser apparatus (Bio-Rad) , .

    Techniques: Infection, Derivative Assay

    Genetic organization of ORFs in the contig harboring the tetracycline resistance gene tet (S) from the genome sequence of S. thermophilus St-9 (A) and in the contig harboring the erythromycin resistance gene ermB from the genome of S. thermophilus St-5 (B) . Color code of the ORFs: in yellow, ORFs complete and/or incomplete ORFs related to transposases, invertases, and topoisomerases; in purple, ORFs involved in antibiotic resistance; in light blue, ORFs related to plasmid segregation and stability; in green, ORF involved in conjugation/mobilization; in white, ORFs encoding hypothetical proteins or proteins coding for other systems. Specific features of (B) : (i) the position of oriT is also indicated; (ii) arrowheads represent a region of direct repeats (DR) in front of the replication module, which showed no significant homology to those in pRE25 and pSM19035; (iii) long segments of the contig (black bars) highly similar ( > 99% nucleotide identity) or identical to plasmid sequences of pRE25 from Enterococcus faecalis and pSM19035 from Streptococcus pyogenes , as indicated; (iv) numbering in plasmids indicates start and end positions of regions of homology to sequences in the contig; (v) purple bars, constituted by truncated insertion sequence (IS) elements, separate non-colinear regions of homology; (vi) blue bars indicate regions of low homology to those found in pRE25 and pSM19035; (vii) the broken line indicates sequences not present in pSM19035.

    Journal: Frontiers in Microbiology

    Article Title: Antibiotic Resistance-Susceptibility Profiles of Streptococcus thermophilus Isolated from Raw Milk and Genome Analysis of the Genetic Basis of Acquired Resistances

    doi: 10.3389/fmicb.2017.02608

    Figure Lengend Snippet: Genetic organization of ORFs in the contig harboring the tetracycline resistance gene tet (S) from the genome sequence of S. thermophilus St-9 (A) and in the contig harboring the erythromycin resistance gene ermB from the genome of S. thermophilus St-5 (B) . Color code of the ORFs: in yellow, ORFs complete and/or incomplete ORFs related to transposases, invertases, and topoisomerases; in purple, ORFs involved in antibiotic resistance; in light blue, ORFs related to plasmid segregation and stability; in green, ORF involved in conjugation/mobilization; in white, ORFs encoding hypothetical proteins or proteins coding for other systems. Specific features of (B) : (i) the position of oriT is also indicated; (ii) arrowheads represent a region of direct repeats (DR) in front of the replication module, which showed no significant homology to those in pRE25 and pSM19035; (iii) long segments of the contig (black bars) highly similar ( > 99% nucleotide identity) or identical to plasmid sequences of pRE25 from Enterococcus faecalis and pSM19035 from Streptococcus pyogenes , as indicated; (iv) numbering in plasmids indicates start and end positions of regions of homology to sequences in the contig; (v) purple bars, constituted by truncated insertion sequence (IS) elements, separate non-colinear regions of homology; (vi) blue bars indicate regions of low homology to those found in pRE25 and pSM19035; (vii) the broken line indicates sequences not present in pSM19035.

    Article Snippet: Unless otherwise stated, S. thermophilus strains were grown statically under anaerobic conditions in M17 (Oxoid, Basingstoke, UK) with 0.5% lactose (LM17) or Mueller-Hinton (Oxoid) at 37°C for 24–48 h. L. plantarum and E. faecalis were grown under aerobic conditions at 30°C in de Man Rogosa and Sharpe (MRS) broth (Merck, Darmstad, Germany).

    Techniques: Sequencing, Plasmid Preparation, Conjugation Assay

    In vitro antibiofilm and antibacterial activity of Enterococcus faecalis : ( A ) BA and derivatives; ( B ) UA and derivatives. The results represent the mean ± standard deviation of five experiments. * Represents a significant difference in relation to the biofilm formation control ( p

    Journal: Biomolecules

    Article Title: Triterpene Derivatives as Relevant Scaffold for New Antibiofilm Drugs

    doi: 10.3390/biom9020058

    Figure Lengend Snippet: In vitro antibiofilm and antibacterial activity of Enterococcus faecalis : ( A ) BA and derivatives; ( B ) UA and derivatives. The results represent the mean ± standard deviation of five experiments. * Represents a significant difference in relation to the biofilm formation control ( p

    Article Snippet: Antibiofilm and Antibacterial Activity Assays A planktonic susceptibility testing of S. epidermidis, S. aureus and E. faecalis was performed by the reference broth microdilution assay according to Clinical and Laboratory Standards Institute (CLSI) guidelines [ ].

    Techniques: In Vitro, Activity Assay, Standard Deviation

    Psh is activated by bacterial proteolytic activities in the hemolymph Toll pathway activation was monitored in adult flies by RNA hybridization analysis of drs messenger expression. Rp49 messenger was used as loading control and for normalization of drs expression. drs mRNA abundance ( drs/Rp49 ) in wild-type ( WT ) flies was set to 100 as a control and values obtained with mutant flies were expressed as percentage of this value. This result is representative of 3 independent experiments and histograms represent the mean of these results. Error bars are SD. ( a,b ) drs expression 24 hours after injection of E. faecalis peptidoglycan is not significantly affected in psh mutant flies ( P = 0.24) but is reduced in grass Herrade ( hrd ) ( P = 2.3 × 10 -6 ) or psh , hrd double mutant flies ( P = 0.009) at a very comparable level ( P = 0.45). ( c,d ) drs expression 24 hours after injection of heat-killed E. faecalis is not significantly affected in psh mutant flies ( P = 0.05) but is highly reduced in hrd single ( P = 1.6 × 10 -5 ) or psh, hrd double mutant flies ( P = 6 × 10 -5 ) at a very comparable level ( P = 0.45). ( e ) drs expression 24 hours after infection of WT flies with living or heat-killed E. faecalis is significantly reduced in flies challenged with dead bacteria ( P = 2.7 × 10 -5 ). ( f,g ) drs expression 24 hours after injection of bacterial subtilisin is not affected in hrd mutant ( P = 0.8) but is highly reduced in psh mutant flies ( P = 1,9 × 10 -8 ). The reduction in drs expression observed in psh is identical to that observed in the hemizygous SPE Pasteur mutant ( pstr /Df1) ( P = 0.58) and is not further enhanced in psh , hrd double mutant flies ( P = 0.1).

    Journal: Nature immunology

    Article Title: Danger signal and PAMP sensing define binary signaling pathways upstream of Toll

    doi: 10.1038/ni.1643

    Figure Lengend Snippet: Psh is activated by bacterial proteolytic activities in the hemolymph Toll pathway activation was monitored in adult flies by RNA hybridization analysis of drs messenger expression. Rp49 messenger was used as loading control and for normalization of drs expression. drs mRNA abundance ( drs/Rp49 ) in wild-type ( WT ) flies was set to 100 as a control and values obtained with mutant flies were expressed as percentage of this value. This result is representative of 3 independent experiments and histograms represent the mean of these results. Error bars are SD. ( a,b ) drs expression 24 hours after injection of E. faecalis peptidoglycan is not significantly affected in psh mutant flies ( P = 0.24) but is reduced in grass Herrade ( hrd ) ( P = 2.3 × 10 -6 ) or psh , hrd double mutant flies ( P = 0.009) at a very comparable level ( P = 0.45). ( c,d ) drs expression 24 hours after injection of heat-killed E. faecalis is not significantly affected in psh mutant flies ( P = 0.05) but is highly reduced in hrd single ( P = 1.6 × 10 -5 ) or psh, hrd double mutant flies ( P = 6 × 10 -5 ) at a very comparable level ( P = 0.45). ( e ) drs expression 24 hours after infection of WT flies with living or heat-killed E. faecalis is significantly reduced in flies challenged with dead bacteria ( P = 2.7 × 10 -5 ). ( f,g ) drs expression 24 hours after injection of bacterial subtilisin is not affected in hrd mutant ( P = 0.8) but is highly reduced in psh mutant flies ( P = 1,9 × 10 -8 ). The reduction in drs expression observed in psh is identical to that observed in the hemizygous SPE Pasteur mutant ( pstr /Df1) ( P = 0.58) and is not further enhanced in psh , hrd double mutant flies ( P = 0.1).

    Article Snippet: Peptidoglycan injection: 9.2 nl of a suspension at 5 mg/ml of sonicated peptidoglycan from M. luteus (Sigma-Aldrich) or E. faecalis (a generous gift from L. Gutmann, Inserm U872 Paris) were injected in the fly body cavity.

    Techniques: Activation Assay, Hybridization, Expressing, Mutagenesis, Injection, Infection

    Grass is involved in sensing both fungal and Gram-positive bacterial infections ( a ) Quantification of RNA hybridization analysis of drosomycin ( drs ) gene expression 24 h after infection with the Gram-positive bacterium M. luteus . Ribosomal protein 49 ( Rp49 ) messenger was used for normalization. drs mRNA expression ( drs/Rp49 ) in wild-type (WT) flies was set to 100 as a control and values obtained with mutant flies were expressed as percentage of this value. Spaetzle (spz) mutant flies were used as Toll pathway mutant control. Each bar represents the mean of 4 independent experiments, error bars are SD. drs expression was reduced in grass Herrade mutant flies ( hrd ) ( P = 1.3 × 10 -9 ). ( b ) Survival of adult flies infected with E. faecalis . This result is representative of three independent experiments. ( c ) Quantification of RNA hybridization analysis of drs gene expression 48 h after infection with the fungus B. bassiana . Each bar represents the mean of 6 independent experiments, error bars are SD. drs expression is reduced in grass Herrade mutant flies ( hrd ) ( P = 0.001). ( d ) Survival of adult flies infected with B. bassiana . This result is representative of three independent experiments.

    Journal: Nature immunology

    Article Title: Danger signal and PAMP sensing define binary signaling pathways upstream of Toll

    doi: 10.1038/ni.1643

    Figure Lengend Snippet: Grass is involved in sensing both fungal and Gram-positive bacterial infections ( a ) Quantification of RNA hybridization analysis of drosomycin ( drs ) gene expression 24 h after infection with the Gram-positive bacterium M. luteus . Ribosomal protein 49 ( Rp49 ) messenger was used for normalization. drs mRNA expression ( drs/Rp49 ) in wild-type (WT) flies was set to 100 as a control and values obtained with mutant flies were expressed as percentage of this value. Spaetzle (spz) mutant flies were used as Toll pathway mutant control. Each bar represents the mean of 4 independent experiments, error bars are SD. drs expression was reduced in grass Herrade mutant flies ( hrd ) ( P = 1.3 × 10 -9 ). ( b ) Survival of adult flies infected with E. faecalis . This result is representative of three independent experiments. ( c ) Quantification of RNA hybridization analysis of drs gene expression 48 h after infection with the fungus B. bassiana . Each bar represents the mean of 6 independent experiments, error bars are SD. drs expression is reduced in grass Herrade mutant flies ( hrd ) ( P = 0.001). ( d ) Survival of adult flies infected with B. bassiana . This result is representative of three independent experiments.

    Article Snippet: Peptidoglycan injection: 9.2 nl of a suspension at 5 mg/ml of sonicated peptidoglycan from M. luteus (Sigma-Aldrich) or E. faecalis (a generous gift from L. Gutmann, Inserm U872 Paris) were injected in the fly body cavity.

    Techniques: Hybridization, Expressing, Infection, Mutagenesis

    Grass and Psh define two parallel pathways that activate Toll cooperatively ( a-f ) Toll pathway activation was monitored by RNA hybridization analysis of drs messenger expression. Rp49 messenger was used as loading control and for normalization of drosomycin ( drs) expression. drs mRNA abundance ( drs/Rp49 ) in wild-type (WT) flies was set to 100 as a control and values obtained with mutant flies were expressed as percentage of this value. RNA hybridizations are representative of 3 independent experiments and histograms represent the mean of these results. Error bars are SD. ( a,b ) drs expression is reduced in grass Herrade ( hrd ) and psh ( P = 0.002 and P = 9 × 10 -4 respectively) 36 h after infection by the fungus B. bassiana. drs expression is reduced in psh , hrd double mutant flies compared to both single mutants ( P = 7 × 10 -4 and P = 0.003 respectively) and is similar to the expression in spz mutants ( P = 0.9). drs expressi on is not significantly different in GNBP3 Hades , hrd ( GNBP3; hrd ) double mutants compared to hrd single mutants ( P = 0.65). ( c ) Survival curves of adult flies infected with B. bassiana. Results are representative of 3 independent experiments. ( d,e ) drs expression 24 hours after infection by the Gram-positive bacterium M. luteus is significantly reduced in psh, hrd double mutant compared to hrd single mutant flies ( p = 0.003) and is similar to that detected in spz mutants ( P = 0.45). drs expression is significantly reduced in psh mutants ( P = 0.002). ( f,g ) drs expression 24 hours after infection by the Gram-positive bacterium E. faecalis is significantly reduced in psh, hrd double mutant flies ( P = 1.5 10 -7 ) and only slightly reduced in hrd, psh or GNBP1 Osiris ( GNBP1 ) single mutant flies ( P = 0.18, P = 0.16 and P = 0.08 espectively). drs expression in psh , hrd double mutant is similar to that in spz mutants ( P = 0.12) and not significantly different in GNBP1 Osiris , hrd ( GNBP1; hrd ) double mutant compared to hrd single mutant ( P = 0.6). (h) Survival curves of adult flies infected with E. faecalis . Results are representative of 3 independent experiments.

    Journal: Nature immunology

    Article Title: Danger signal and PAMP sensing define binary signaling pathways upstream of Toll

    doi: 10.1038/ni.1643

    Figure Lengend Snippet: Grass and Psh define two parallel pathways that activate Toll cooperatively ( a-f ) Toll pathway activation was monitored by RNA hybridization analysis of drs messenger expression. Rp49 messenger was used as loading control and for normalization of drosomycin ( drs) expression. drs mRNA abundance ( drs/Rp49 ) in wild-type (WT) flies was set to 100 as a control and values obtained with mutant flies were expressed as percentage of this value. RNA hybridizations are representative of 3 independent experiments and histograms represent the mean of these results. Error bars are SD. ( a,b ) drs expression is reduced in grass Herrade ( hrd ) and psh ( P = 0.002 and P = 9 × 10 -4 respectively) 36 h after infection by the fungus B. bassiana. drs expression is reduced in psh , hrd double mutant flies compared to both single mutants ( P = 7 × 10 -4 and P = 0.003 respectively) and is similar to the expression in spz mutants ( P = 0.9). drs expressi on is not significantly different in GNBP3 Hades , hrd ( GNBP3; hrd ) double mutants compared to hrd single mutants ( P = 0.65). ( c ) Survival curves of adult flies infected with B. bassiana. Results are representative of 3 independent experiments. ( d,e ) drs expression 24 hours after infection by the Gram-positive bacterium M. luteus is significantly reduced in psh, hrd double mutant compared to hrd single mutant flies ( p = 0.003) and is similar to that detected in spz mutants ( P = 0.45). drs expression is significantly reduced in psh mutants ( P = 0.002). ( f,g ) drs expression 24 hours after infection by the Gram-positive bacterium E. faecalis is significantly reduced in psh, hrd double mutant flies ( P = 1.5 10 -7 ) and only slightly reduced in hrd, psh or GNBP1 Osiris ( GNBP1 ) single mutant flies ( P = 0.18, P = 0.16 and P = 0.08 espectively). drs expression in psh , hrd double mutant is similar to that in spz mutants ( P = 0.12) and not significantly different in GNBP1 Osiris , hrd ( GNBP1; hrd ) double mutant compared to hrd single mutant ( P = 0.6). (h) Survival curves of adult flies infected with E. faecalis . Results are representative of 3 independent experiments.

    Article Snippet: Peptidoglycan injection: 9.2 nl of a suspension at 5 mg/ml of sonicated peptidoglycan from M. luteus (Sigma-Aldrich) or E. faecalis (a generous gift from L. Gutmann, Inserm U872 Paris) were injected in the fly body cavity.

    Techniques: Activation Assay, Hybridization, Expressing, Mutagenesis, Infection

    The ebp operon in E. faecalis OG1RF encodes the Ebp pilus structural subunits EbpA (blue), EbpB (purple), and EbpC (orange) and the pilus-associated sortase SrtC (green). The housekeeping sortase SrtA (brown) is encoded elsewhere in the genome (A). Sortase recognition motif amino acid sequences (LPETG for EbpA, LPKTN for EbpB, and LPSTG for EbpC), pilin motif Lys residues (K), and the sortase catalytic Cys residues (C) are shown by the one-letter code on their respective subunits. Predicted Srt subunit thioacyl intermediates are faded (A to E). EbpA incorporation relies on cleavage of its sortase recognition motif by SrtC and resolution of the resultant intermediate by the EbpC pilin motif Lys residue (B). EbpC polymerization requires SrtC transpeptidation between the sortase recognition motif of the most recently incorporated EbpC subunit of a growing fiber and the pilin motif Lys residue of an incoming EbpC subunit (C). EbpB is incorporated into a growing fiber via its pilin motif Lys residue (D). SrtA participates in anchoring fully polymerized pilus fibers to the cell wall, likely via transpeptidation of the EbpB sortase recognition motif with a cell wall precursor molecule.

    Journal: Journal of Bacteriology

    Article Title: Pilin and Sortase Residues Critical for Endocarditis- and Biofilm-Associated Pilus Biogenesis in Enterococcus faecalis

    doi: 10.1128/JB.00451-13

    Figure Lengend Snippet: The ebp operon in E. faecalis OG1RF encodes the Ebp pilus structural subunits EbpA (blue), EbpB (purple), and EbpC (orange) and the pilus-associated sortase SrtC (green). The housekeeping sortase SrtA (brown) is encoded elsewhere in the genome (A). Sortase recognition motif amino acid sequences (LPETG for EbpA, LPKTN for EbpB, and LPSTG for EbpC), pilin motif Lys residues (K), and the sortase catalytic Cys residues (C) are shown by the one-letter code on their respective subunits. Predicted Srt subunit thioacyl intermediates are faded (A to E). EbpA incorporation relies on cleavage of its sortase recognition motif by SrtC and resolution of the resultant intermediate by the EbpC pilin motif Lys residue (B). EbpC polymerization requires SrtC transpeptidation between the sortase recognition motif of the most recently incorporated EbpC subunit of a growing fiber and the pilin motif Lys residue of an incoming EbpC subunit (C). EbpB is incorporated into a growing fiber via its pilin motif Lys residue (D). SrtA participates in anchoring fully polymerized pilus fibers to the cell wall, likely via transpeptidation of the EbpB sortase recognition motif with a cell wall precursor molecule.

    Article Snippet: Expression plasmids were maintained in E. faecalis by supplementation of growth media with 500 μg ml−1 Kan. All antibiotics were purchased from Sigma-Aldrich Corporation (St. Louis, MO).

    Techniques:

    Time-course profile of growth, glucose consumption and lactate production by E . faecalis CBRD01 under anaerobic batch fermentation.A. Glucose consumption, biomass and lactate production.B. Specific rates of glucose uptake and lactate production.C. Volumetric productivity of lactate.

    Journal: Microbial Biotechnology

    Article Title: Production of lactic acid using a new homofermentative Enterococcus faecalis isolate

    doi: 10.1111/1751-7915.12133

    Figure Lengend Snippet: Time-course profile of growth, glucose consumption and lactate production by E . faecalis CBRD01 under anaerobic batch fermentation.A. Glucose consumption, biomass and lactate production.B. Specific rates of glucose uptake and lactate production.C. Volumetric productivity of lactate.

    Article Snippet: The strain CBRD01 was biochemically characterized (Table ) and identified as E. faecalis by DSMZ-Germany ( http://www.dsmz.de/ ).

    Techniques:

    Time-course profile of growth, glucose consumption and lactate production by E . faecalis CBRD01 under anaerobic fed-batch fermentation. Arrows indicate addition of yeast extract at 5 g l −1 . The numbers 1–14 in grey indicate glucose addition at different time intervals and different concentrations (1–86.4 mM; 2–83.74 mM; 3–42.07 mM; 4–14.63 mM; 5–46.04 mM; 6–83.59 mM; 7–33.82 mM; 8–33.17 mM; 9–45.71 mM; 10–46.98 mM; 11–82.34 mM; 12–82.80 mM; 13–73.24 mM; and 14–70.75 mM).

    Journal: Microbial Biotechnology

    Article Title: Production of lactic acid using a new homofermentative Enterococcus faecalis isolate

    doi: 10.1111/1751-7915.12133

    Figure Lengend Snippet: Time-course profile of growth, glucose consumption and lactate production by E . faecalis CBRD01 under anaerobic fed-batch fermentation. Arrows indicate addition of yeast extract at 5 g l −1 . The numbers 1–14 in grey indicate glucose addition at different time intervals and different concentrations (1–86.4 mM; 2–83.74 mM; 3–42.07 mM; 4–14.63 mM; 5–46.04 mM; 6–83.59 mM; 7–33.82 mM; 8–33.17 mM; 9–45.71 mM; 10–46.98 mM; 11–82.34 mM; 12–82.80 mM; 13–73.24 mM; and 14–70.75 mM).

    Article Snippet: The strain CBRD01 was biochemically characterized (Table ) and identified as E. faecalis by DSMZ-Germany ( http://www.dsmz.de/ ).

    Techniques:

    Proposed metabolic pathway for lactate production from glucose in E . faecalis CBRD01.

    Journal: Microbial Biotechnology

    Article Title: Production of lactic acid using a new homofermentative Enterococcus faecalis isolate

    doi: 10.1111/1751-7915.12133

    Figure Lengend Snippet: Proposed metabolic pathway for lactate production from glucose in E . faecalis CBRD01.

    Article Snippet: The strain CBRD01 was biochemically characterized (Table ) and identified as E. faecalis by DSMZ-Germany ( http://www.dsmz.de/ ).

    Techniques:

    Time-course profile of growth, glucose consumption and lactate production by E . faecalis CBRD01under anaerobic fed-batch fermentation with initial cell density of 22 g l −1 . Arrow indicates glucose addition.

    Journal: Microbial Biotechnology

    Article Title: Production of lactic acid using a new homofermentative Enterococcus faecalis isolate

    doi: 10.1111/1751-7915.12133

    Figure Lengend Snippet: Time-course profile of growth, glucose consumption and lactate production by E . faecalis CBRD01under anaerobic fed-batch fermentation with initial cell density of 22 g l −1 . Arrow indicates glucose addition.

    Article Snippet: The strain CBRD01 was biochemically characterized (Table ) and identified as E. faecalis by DSMZ-Germany ( http://www.dsmz.de/ ).

    Techniques:

    (A and B) MCs derived from wild-type TLR2 −/− or MyD88 −/− mice were impaired in their capacity to degranulate (A) and to inhibit growth of E. faecalis (120 min of infection) (B). Each bar represents the mean ± SD

    Journal: Infection and Immunity

    Article Title: New Insights into the Antimicrobial Effect of Mast Cells against Enterococcus faecalis

    doi: 10.1128/IAI.02114-14

    Figure Lengend Snippet: (A and B) MCs derived from wild-type TLR2 −/− or MyD88 −/− mice were impaired in their capacity to degranulate (A) and to inhibit growth of E. faecalis (120 min of infection) (B). Each bar represents the mean ± SD

    Article Snippet: For some experiments, E. faecalis was cocultivated with LL-37 (AnaSpec, San Jose, CA) added for 90 min.

    Techniques: Derivative Assay, Mouse Assay, Infection

    (A and B) MCs derived from either wild-type, TLR2 −/− , or MyD88 −/− mice were altered in their capacity to release IL-6 (A) and TNF-α (B) in response to E. faecalis . Each bar represents the mean ± SD of three

    Journal: Infection and Immunity

    Article Title: New Insights into the Antimicrobial Effect of Mast Cells against Enterococcus faecalis

    doi: 10.1128/IAI.02114-14

    Figure Lengend Snippet: (A and B) MCs derived from either wild-type, TLR2 −/− , or MyD88 −/− mice were altered in their capacity to release IL-6 (A) and TNF-α (B) in response to E. faecalis . Each bar represents the mean ± SD of three

    Article Snippet: For some experiments, E. faecalis was cocultivated with LL-37 (AnaSpec, San Jose, CA) added for 90 min.

    Techniques: Derivative Assay, Mouse Assay

    Contribution of granule components released by MCs to the antimicrobial effect against E. faecalis . (A and B) Uninfected MCs (A) or MCs infected for 3 h with E. faecalis (B) were fixed and stained with toluidine blue. (C to E) Scanning electron photographs

    Journal: Infection and Immunity

    Article Title: New Insights into the Antimicrobial Effect of Mast Cells against Enterococcus faecalis

    doi: 10.1128/IAI.02114-14

    Figure Lengend Snippet: Contribution of granule components released by MCs to the antimicrobial effect against E. faecalis . (A and B) Uninfected MCs (A) or MCs infected for 3 h with E. faecalis (B) were fixed and stained with toluidine blue. (C to E) Scanning electron photographs

    Article Snippet: For some experiments, E. faecalis was cocultivated with LL-37 (AnaSpec, San Jose, CA) added for 90 min.

    Techniques: Infection, Staining

    Double-immunofluorescence staining of E. faecalis -infected MCs (A) and MC released extracellular traps after exposure to E. faecalis . MCs were infected with E. faecalis at an MOI of 10 to 1 for 90 min and stained for double immunofluorescence. Extracellular

    Journal: Infection and Immunity

    Article Title: New Insights into the Antimicrobial Effect of Mast Cells against Enterococcus faecalis

    doi: 10.1128/IAI.02114-14

    Figure Lengend Snippet: Double-immunofluorescence staining of E. faecalis -infected MCs (A) and MC released extracellular traps after exposure to E. faecalis . MCs were infected with E. faecalis at an MOI of 10 to 1 for 90 min and stained for double immunofluorescence. Extracellular

    Article Snippet: For some experiments, E. faecalis was cocultivated with LL-37 (AnaSpec, San Jose, CA) added for 90 min.

    Techniques: Double Immunofluorescence Staining, Infection, Staining, Immunofluorescence

    Association of E. faecalis with MCs. (A) Scanning microscopy picture of E. faecalis attached to the surface of MCs (bacteria are indicated by white arrows). (B) High magnification of an MC harboring E. faecalis (white arrow). Bars represent 3 μm

    Journal: Infection and Immunity

    Article Title: New Insights into the Antimicrobial Effect of Mast Cells against Enterococcus faecalis

    doi: 10.1128/IAI.02114-14

    Figure Lengend Snippet: Association of E. faecalis with MCs. (A) Scanning microscopy picture of E. faecalis attached to the surface of MCs (bacteria are indicated by white arrows). (B) High magnification of an MC harboring E. faecalis (white arrow). Bars represent 3 μm

    Article Snippet: For some experiments, E. faecalis was cocultivated with LL-37 (AnaSpec, San Jose, CA) added for 90 min.

    Techniques: Microscopy

    Antimicrobial effect of LL-37 against E. faecalis . (A) Growth of E. faecalis in the presence of different concentrations of cathelicidin LL-37 for 120 min. Each bar represents the mean ± SD of quadruplicates from two independent experiments. *,

    Journal: Infection and Immunity

    Article Title: New Insights into the Antimicrobial Effect of Mast Cells against Enterococcus faecalis

    doi: 10.1128/IAI.02114-14

    Figure Lengend Snippet: Antimicrobial effect of LL-37 against E. faecalis . (A) Growth of E. faecalis in the presence of different concentrations of cathelicidin LL-37 for 120 min. Each bar represents the mean ± SD of quadruplicates from two independent experiments. *,

    Article Snippet: For some experiments, E. faecalis was cocultivated with LL-37 (AnaSpec, San Jose, CA) added for 90 min.

    Techniques:

    LIVE/DEAD staining of MCs infected with E. faecalis . (A and B) MCs were seeded on poly- l -lysine coated glass slides, then infected with E. faecalis for 3 h at an MOI of 10:1, fixed with 4% paraformaldehyde, and examined by immunofluorescence microscopy

    Journal: Infection and Immunity

    Article Title: New Insights into the Antimicrobial Effect of Mast Cells against Enterococcus faecalis

    doi: 10.1128/IAI.02114-14

    Figure Lengend Snippet: LIVE/DEAD staining of MCs infected with E. faecalis . (A and B) MCs were seeded on poly- l -lysine coated glass slides, then infected with E. faecalis for 3 h at an MOI of 10:1, fixed with 4% paraformaldehyde, and examined by immunofluorescence microscopy

    Article Snippet: For some experiments, E. faecalis was cocultivated with LL-37 (AnaSpec, San Jose, CA) added for 90 min.

    Techniques: Staining, Infection, Immunofluorescence, Microscopy

    Schematic diagram of root canal sample preparation. (A) Single rooted teeth; the area from which the dentin block is prepared marked with red. (B) Standardized 4 mm root dentin block. (C) Standardized root canal diameter preparation with size 6 Gates Glidden (1.5 mm diameter). (D) The vertical direction of block splitting into to semi cylindrical halves. (E) The outer surfaces of the semicylindrical halves (the cemental side) were ground to achieve a standard thickness of 2 mm and to remove the root surface cement. (F) The specimen was inoculated with an overnight suspension of E. faecalis in BHI (2 ml) adjusted spectrophotometrically to OD 600 of 0.5, which was grown for 3 weeks. (G) Disinfecting the sample with different control and test irrigants. (H) Splitting the canal surface to label freshly exposed DT with LIVE/DEAD BacLight staining and examine them under CLSM.

    Journal: Journal of endodontics

    Article Title: Novel Endodontic Disinfection Approach Using Catalytic Nanoparticles.

    doi: 10.1016/j.joen.2017.12.003

    Figure Lengend Snippet: Schematic diagram of root canal sample preparation. (A) Single rooted teeth; the area from which the dentin block is prepared marked with red. (B) Standardized 4 mm root dentin block. (C) Standardized root canal diameter preparation with size 6 Gates Glidden (1.5 mm diameter). (D) The vertical direction of block splitting into to semi cylindrical halves. (E) The outer surfaces of the semicylindrical halves (the cemental side) were ground to achieve a standard thickness of 2 mm and to remove the root surface cement. (F) The specimen was inoculated with an overnight suspension of E. faecalis in BHI (2 ml) adjusted spectrophotometrically to OD 600 of 0.5, which was grown for 3 weeks. (G) Disinfecting the sample with different control and test irrigants. (H) Splitting the canal surface to label freshly exposed DT with LIVE/DEAD BacLight staining and examine them under CLSM.

    Article Snippet: Five hundred microliters of the adjusted E. faecalis suspension was then added to each well (Olympus 24 well plate, 3.5 ml, Genesee Scientific.

    Techniques: Sample Prep, Blocking Assay, Staining, Confocal Laser Scanning Microscopy

    LAMP assays of 10 5 E. faecalis non-lysed cells (with and without PMA treatment) and PMA treated lysed cells on a commercial PCR instrument.

    Journal: Journal of microbiological methods

    Article Title: Most Probable Number - Loop Mediated Isothermal Amplification (MPN-LAMP) for Quantifying Waterborne Pathogens in Less Than 25 Minutes

    doi: 10.1016/j.mimet.2016.11.010

    Figure Lengend Snippet: LAMP assays of 10 5 E. faecalis non-lysed cells (with and without PMA treatment) and PMA treated lysed cells on a commercial PCR instrument.

    Article Snippet: Additionally, Tt values for E. coli and E. faecalis assays on the microchip (5 s CCD exposure) was higher by 2.3 min and 4.2 min, respectively, than the assays on commercial PCR instrument.

    Techniques: Polymerase Chain Reaction

    MPN-LAMP image for (a) E. coli ( gadA gene) and (b) E. faecalis ( gelE gene). Cell number per well (left) and number of positive reaction (right) were shown in the image.

    Journal: Journal of microbiological methods

    Article Title: Most Probable Number - Loop Mediated Isothermal Amplification (MPN-LAMP) for Quantifying Waterborne Pathogens in Less Than 25 Minutes

    doi: 10.1016/j.mimet.2016.11.010

    Figure Lengend Snippet: MPN-LAMP image for (a) E. coli ( gadA gene) and (b) E. faecalis ( gelE gene). Cell number per well (left) and number of positive reaction (right) were shown in the image.

    Article Snippet: Additionally, Tt values for E. coli and E. faecalis assays on the microchip (5 s CCD exposure) was higher by 2.3 min and 4.2 min, respectively, than the assays on commercial PCR instrument.

    Techniques:

    (b) Standard curves of RT f -LAMP assays of E. coli ( uidA gene) and E. faecalis ( gelE gene) cells on a commercial PCR instrument.

    Journal: Journal of microbiological methods

    Article Title: Most Probable Number - Loop Mediated Isothermal Amplification (MPN-LAMP) for Quantifying Waterborne Pathogens in Less Than 25 Minutes

    doi: 10.1016/j.mimet.2016.11.010

    Figure Lengend Snippet: (b) Standard curves of RT f -LAMP assays of E. coli ( uidA gene) and E. faecalis ( gelE gene) cells on a commercial PCR instrument.

    Article Snippet: Additionally, Tt values for E. coli and E. faecalis assays on the microchip (5 s CCD exposure) was higher by 2.3 min and 4.2 min, respectively, than the assays on commercial PCR instrument.

    Techniques: Polymerase Chain Reaction

    MPN-LAMP assays of single digit CFU of E. coli ( uidA gene) and E. faecalis ( gelE gene). Inset showed the CCD time lapse images of microchip with 10 sample replicates and 10 negative controls. The error bars represented the standard deviations of the mean from 5 and 8 positive replicates (amplified) and 10 negative replicates for E. coli and E. faecalis, respectively.

    Journal: Journal of microbiological methods

    Article Title: Most Probable Number - Loop Mediated Isothermal Amplification (MPN-LAMP) for Quantifying Waterborne Pathogens in Less Than 25 Minutes

    doi: 10.1016/j.mimet.2016.11.010

    Figure Lengend Snippet: MPN-LAMP assays of single digit CFU of E. coli ( uidA gene) and E. faecalis ( gelE gene). Inset showed the CCD time lapse images of microchip with 10 sample replicates and 10 negative controls. The error bars represented the standard deviations of the mean from 5 and 8 positive replicates (amplified) and 10 negative replicates for E. coli and E. faecalis, respectively.

    Article Snippet: Additionally, Tt values for E. coli and E. faecalis assays on the microchip (5 s CCD exposure) was higher by 2.3 min and 4.2 min, respectively, than the assays on commercial PCR instrument.

    Techniques: MicroChIP Assay, Amplification

    Phylogenetic relationship among selected E. faecalis strains based on whole genome alignments. The alignment was calculated with Mugsy ( http://mugsy.sourceforge.net/ [last access 16.07.2014] [ 57 ]) and only aligned regions present in all analyzed strains were extracted. These regions were concatenated and positions with gaps removed. The resulting core alignment was used to infer a Maximum Likelihood tree with RAxML. The GTRGAMMA model for nucleotide substitution and rate heterogeneity was utilized, bootstrap support values of 1000 replicates are shown at the nodes. Names of the ST40 isolates and their origin are indicated at the end of the branches. The highly related ST40 isolates were further zoomed in exemplified by the dotted line and the different scale bar. Metadata are given as follows: Strain no., year of isolation, origin, country: AC, animal colonizer; AI, animal infection; HC, human colonizer; HI, human infection; CU, Cuba; D, Germany; DK, Denmark; ES, Spain; IS, Island; PL, Poland; USA.

    Journal: BMC Genomics

    Article Title: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

    doi: 10.1186/s12864-015-1367-x

    Figure Lengend Snippet: Phylogenetic relationship among selected E. faecalis strains based on whole genome alignments. The alignment was calculated with Mugsy ( http://mugsy.sourceforge.net/ [last access 16.07.2014] [ 57 ]) and only aligned regions present in all analyzed strains were extracted. These regions were concatenated and positions with gaps removed. The resulting core alignment was used to infer a Maximum Likelihood tree with RAxML. The GTRGAMMA model for nucleotide substitution and rate heterogeneity was utilized, bootstrap support values of 1000 replicates are shown at the nodes. Names of the ST40 isolates and their origin are indicated at the end of the branches. The highly related ST40 isolates were further zoomed in exemplified by the dotted line and the different scale bar. Metadata are given as follows: Strain no., year of isolation, origin, country: AC, animal colonizer; AI, animal infection; HC, human colonizer; HI, human infection; CU, Cuba; D, Germany; DK, Denmark; ES, Spain; IS, Island; PL, Poland; USA.

    Article Snippet: Conclusion Our detailed molecular and phenotypic analyses of 42 E. faecalis strains of MLST type ST40 and the genomic analyses of a subset of 15 isolates revealed a minor level of genomic diversity.

    Techniques: Isolation, Infection

    Comparative analysis of the finished and publicly available E. faecalis genomes. EDGAR generated Venn diagram facilitates visualizing core and strain-specific (“unique”) genes. This comparative analysis only exploits CDS of the chromosomes without considering of plasmid genes, whereby all strains shared 2173 CDS; 1, E. faecalis strain 62 (CP002491); 2, E. faecalis strain OG1RF (CP002621); 3, E. faecalis V583 (NC_004668); 4, E. faecalis probiotic strain Symbioflor 1 Clone DSM 16431 (NC_019770); 5, E. faecalis strain D32 (CP003726).

    Journal: BMC Genomics

    Article Title: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

    doi: 10.1186/s12864-015-1367-x

    Figure Lengend Snippet: Comparative analysis of the finished and publicly available E. faecalis genomes. EDGAR generated Venn diagram facilitates visualizing core and strain-specific (“unique”) genes. This comparative analysis only exploits CDS of the chromosomes without considering of plasmid genes, whereby all strains shared 2173 CDS; 1, E. faecalis strain 62 (CP002491); 2, E. faecalis strain OG1RF (CP002621); 3, E. faecalis V583 (NC_004668); 4, E. faecalis probiotic strain Symbioflor 1 Clone DSM 16431 (NC_019770); 5, E. faecalis strain D32 (CP003726).

    Article Snippet: Conclusion Our detailed molecular and phenotypic analyses of 42 E. faecalis strains of MLST type ST40 and the genomic analyses of a subset of 15 isolates revealed a minor level of genomic diversity.

    Techniques: Generated, Plasmid Preparation

    Pathogenicity of E. faecalis D32 and UW7709 in a Galleria mellonella model. Death rates of G. mellonella larvae after injection with E. faecalis strains D32 (real infectious dose: 1.7 × 10 5 CFU per larvae) and UW7709 (real infectious dose: 2.8 × 10 5 CFU per larvae), respectively. PBS injection served as a negative control. One representative experiment of three independent experiments is shown. Data are displayed by as Kaplan-Meier plot survival curves. Statistical significance (p

    Journal: BMC Genomics

    Article Title: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

    doi: 10.1186/s12864-015-1367-x

    Figure Lengend Snippet: Pathogenicity of E. faecalis D32 and UW7709 in a Galleria mellonella model. Death rates of G. mellonella larvae after injection with E. faecalis strains D32 (real infectious dose: 1.7 × 10 5 CFU per larvae) and UW7709 (real infectious dose: 2.8 × 10 5 CFU per larvae), respectively. PBS injection served as a negative control. One representative experiment of three independent experiments is shown. Data are displayed by as Kaplan-Meier plot survival curves. Statistical significance (p

    Article Snippet: Conclusion Our detailed molecular and phenotypic analyses of 42 E. faecalis strains of MLST type ST40 and the genomic analyses of a subset of 15 isolates revealed a minor level of genomic diversity.

    Techniques: Injection, Negative Control

    Growth rates of E. faecalis UW7709 and D32 in a mouse bacteremia model. Eight female BALB/c mice were infected via the tail vein with E. faecalis strains UW7709 or D32 (5 × 10 8 CFU). After 48 hours, mice were sacrificed and bacterial counts in (A) liver, kidneys and spleen, as well as, in (B) blood were determined. Data represent the individual bacterial burdens and the geometric mean values. Asterisks indicate significant P values calculated by using Mann–Whitney test (* P

    Journal: BMC Genomics

    Article Title: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

    doi: 10.1186/s12864-015-1367-x

    Figure Lengend Snippet: Growth rates of E. faecalis UW7709 and D32 in a mouse bacteremia model. Eight female BALB/c mice were infected via the tail vein with E. faecalis strains UW7709 or D32 (5 × 10 8 CFU). After 48 hours, mice were sacrificed and bacterial counts in (A) liver, kidneys and spleen, as well as, in (B) blood were determined. Data represent the individual bacterial burdens and the geometric mean values. Asterisks indicate significant P values calculated by using Mann–Whitney test (* P

    Article Snippet: Conclusion Our detailed molecular and phenotypic analyses of 42 E. faecalis strains of MLST type ST40 and the genomic analyses of a subset of 15 isolates revealed a minor level of genomic diversity.

    Techniques: Mouse Assay, Infection, MANN-WHITNEY

    E. faecalis ST40 genome comparison against the D32 reference genome. Generated by BRIG ( http://brig.sourceforge.net/ [last access 16.07.2014] [ 56 ]), the circular map illustrates the whole genome comparison of D32 against the other 14 sequenced ST40 isolates and the probiotic isolate Symbioflor 1 Clone DSM 16431. The outer cycle (dark grey) represents the complete genome of the reference strain D32. The shade of color is geared to similarities in origin of the strains (green: isolate from bovine mastitis; blue: animal and human commensals; violet: isolates from human infections; red: human blood culture isolates; turquoise: strain Symbiolfor 1). The inner cycle illustrates the GC content of D32. Location of the PAI is illustrated by a blue colored box, while the red box indicates the presence of an uncharacterized and large genomic island (GI; 138 kb). Additionally, black labels highlighted four identified prophages of D32; A, animal; B, blood culture; C, colonizer; E, endocarditis; H, human; M, bovine mastitis; U, urine.

    Journal: BMC Genomics

    Article Title: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

    doi: 10.1186/s12864-015-1367-x

    Figure Lengend Snippet: E. faecalis ST40 genome comparison against the D32 reference genome. Generated by BRIG ( http://brig.sourceforge.net/ [last access 16.07.2014] [ 56 ]), the circular map illustrates the whole genome comparison of D32 against the other 14 sequenced ST40 isolates and the probiotic isolate Symbioflor 1 Clone DSM 16431. The outer cycle (dark grey) represents the complete genome of the reference strain D32. The shade of color is geared to similarities in origin of the strains (green: isolate from bovine mastitis; blue: animal and human commensals; violet: isolates from human infections; red: human blood culture isolates; turquoise: strain Symbiolfor 1). The inner cycle illustrates the GC content of D32. Location of the PAI is illustrated by a blue colored box, while the red box indicates the presence of an uncharacterized and large genomic island (GI; 138 kb). Additionally, black labels highlighted four identified prophages of D32; A, animal; B, blood culture; C, colonizer; E, endocarditis; H, human; M, bovine mastitis; U, urine.

    Article Snippet: Conclusion Our detailed molecular and phenotypic analyses of 42 E. faecalis strains of MLST type ST40 and the genomic analyses of a subset of 15 isolates revealed a minor level of genomic diversity.

    Techniques: Generated

    In vitro biofilm formation. Biofilm formation of E. faecalis strains D32, UW7709 and the internal controls V583, OG1RF and E. faecium strain 64/3 on a synthetic surface was investigated by using polystyrene plates. After incubation in TSB for 24 hours, produced biofilms of adherent bacteria were stained with crystal violet. Bars represent the mean values of six or three (D32) replicates ± SEM. *** significant P -value

    Journal: BMC Genomics

    Article Title: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

    doi: 10.1186/s12864-015-1367-x

    Figure Lengend Snippet: In vitro biofilm formation. Biofilm formation of E. faecalis strains D32, UW7709 and the internal controls V583, OG1RF and E. faecium strain 64/3 on a synthetic surface was investigated by using polystyrene plates. After incubation in TSB for 24 hours, produced biofilms of adherent bacteria were stained with crystal violet. Bars represent the mean values of six or three (D32) replicates ± SEM. *** significant P -value

    Article Snippet: Conclusion Our detailed molecular and phenotypic analyses of 42 E. faecalis strains of MLST type ST40 and the genomic analyses of a subset of 15 isolates revealed a minor level of genomic diversity.

    Techniques: In Vitro, Incubation, Produced, Staining

    Sma I macrorestriction patterns in PFGE of 42 E. faecalis ST40 isolates. The phylogenetic analysis was generated with BioNumerics 6.0. The scale bar represents the level of similiarity in % using default settings (tolerance 1.0; optimization 0.5). The dotted vertical line at 82% similarity delineates the breakpoint for defining related fragment patterns/strains according to an international agreement. Certain sub-clusters indicated a comparably high level of clonal relatedness. Sequenced isolates are marked with a black square. Legend: H, human; A, animal; F, food; 1 reference strain; B, blood culture; CSF, cerebrospinal fluid; C, colonizer; E, endocarditis; F, food; M, bovine mastitis; PF, peritoneal fluid; U, urine; W, wound. Origin: D, Germany; DK, Denmark; PL, Poland; IS, Island; GR, Greece; ESP, Spain.

    Journal: BMC Genomics

    Article Title: Comprehensive molecular, genomic and phenotypic analysis of a major clone of Enterococcus faecalis MLST ST40

    doi: 10.1186/s12864-015-1367-x

    Figure Lengend Snippet: Sma I macrorestriction patterns in PFGE of 42 E. faecalis ST40 isolates. The phylogenetic analysis was generated with BioNumerics 6.0. The scale bar represents the level of similiarity in % using default settings (tolerance 1.0; optimization 0.5). The dotted vertical line at 82% similarity delineates the breakpoint for defining related fragment patterns/strains according to an international agreement. Certain sub-clusters indicated a comparably high level of clonal relatedness. Sequenced isolates are marked with a black square. Legend: H, human; A, animal; F, food; 1 reference strain; B, blood culture; CSF, cerebrospinal fluid; C, colonizer; E, endocarditis; F, food; M, bovine mastitis; PF, peritoneal fluid; U, urine; W, wound. Origin: D, Germany; DK, Denmark; PL, Poland; IS, Island; GR, Greece; ESP, Spain.

    Article Snippet: Conclusion Our detailed molecular and phenotypic analyses of 42 E. faecalis strains of MLST type ST40 and the genomic analyses of a subset of 15 isolates revealed a minor level of genomic diversity.

    Techniques: Generated, End-sequence Profiling

    E. faecalis ).

    Journal: Journal of Bacteriology

    Article Title: Enterococcus faecalis Acetoacetyl-Coenzyme A Thiolase/3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase, a Dual-Function Protein of Isopentenyl Diphosphate Biosynthesis †

    doi: 10.1128/JB.184.8.2116-2122.2002

    Figure Lengend Snippet: E. faecalis ).

    Article Snippet: Antibodies against the E. faecalis fusion protein were raised in New Zealand white rabbits by Covance Research Products, Denver, Pa. Western blotting employed the NOVEX NuPAGE System (Invitrogen Corp.) and the ECL Western blotting system (Amersham Pharmacia Biotech).

    Techniques:

    SDS-PAGE of the expressed mvaE gene product purified by nickel affinity chromatography. S, standards of the indicated mass. Lane 1, early fractions of E. faecalis acetoacetyl-CoA thiolase/HMG-CoA reductase. Numbers indicate molecular sizes in kilodaltons.

    Journal: Journal of Bacteriology

    Article Title: Enterococcus faecalis Acetoacetyl-Coenzyme A Thiolase/3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase, a Dual-Function Protein of Isopentenyl Diphosphate Biosynthesis †

    doi: 10.1128/JB.184.8.2116-2122.2002

    Figure Lengend Snippet: SDS-PAGE of the expressed mvaE gene product purified by nickel affinity chromatography. S, standards of the indicated mass. Lane 1, early fractions of E. faecalis acetoacetyl-CoA thiolase/HMG-CoA reductase. Numbers indicate molecular sizes in kilodaltons.

    Article Snippet: Antibodies against the E. faecalis fusion protein were raised in New Zealand white rabbits by Covance Research Products, Denver, Pa. Western blotting employed the NOVEX NuPAGE System (Invitrogen Corp.) and the ECL Western blotting system (Amersham Pharmacia Biotech).

    Techniques: SDS Page, Purification, Affinity Chromatography

    Agar experimentation showing that all specimens were contaminated by E. Faecalis .

    Journal: BioMed Research International

    Article Title: The Repair of Furcal Perforations in Different Diameters with Biodentine, MTA, and IRM Repair Materials: A Laboratory Study Using an E. Faecalis Leakage Model

    doi: 10.1155/2018/5478796

    Figure Lengend Snippet: Agar experimentation showing that all specimens were contaminated by E. Faecalis .

    Article Snippet: An E. faecalis specific medium (Azide Dextrose Broth, (Oxoid, Ogdensburg, New York, USA.) was placed into the hub to detect the leakage.

    Techniques:

    The blurred colour of the medium indicating the presence of contamination with E. Faecalis .

    Journal: BioMed Research International

    Article Title: The Repair of Furcal Perforations in Different Diameters with Biodentine, MTA, and IRM Repair Materials: A Laboratory Study Using an E. Faecalis Leakage Model

    doi: 10.1155/2018/5478796

    Figure Lengend Snippet: The blurred colour of the medium indicating the presence of contamination with E. Faecalis .

    Article Snippet: An E. faecalis specific medium (Azide Dextrose Broth, (Oxoid, Ogdensburg, New York, USA.) was placed into the hub to detect the leakage.

    Techniques: