Journal: Journal of Bacteriology
Article Title: cis-Acting Elements Responsible for Low-Temperature-Inducible Expression of the Gene Coding for the Thermolabile Isocitrate Dehydrogenase Isozyme of a Psychrophilic Bacterium, Vibrio sp. Strain ABE-1
Figure Lengend Snippet: Growth of E. coli transformants harboring 5′-deleted icdII or an icdII-icdI fusion gene. (A) E. coli DEK2004 was transformed with vector [pBluescript KS(+)] (○), pMS42 (▿), pIS202 (▴), or pMSF8 (▵). (B) E. coli DEK2004 was transformed with pTS601 (⧫), pTS602 (◊), or pSS512 (□). As a control, E. coli XL1-Blue, which has its own icd gene, was also transformed with the vector (●). Other symbols are the same as in panel A. Each transformant, precultured in LB medium at 37°C for 16 h, was inoculated in fresh LB medium and cultured at 15 or 37°C. (C) Specific activity of IDH determined with crude extract prepared from each transformant. Details of assay conditions are described in Materials and Methods. Note the thermolability of IDH-II at 37°C.
Article Snippet: E. coli DEK2004, a mutant defective in icd (a gift from Peter Thorsness), or E. coli XL1-Blue (Stratagene) was used as a host for transformation by icd genes.
Techniques: Transformation Assay, Plasmid Preparation, Cell Culture, Activity Assay