e. coli strains xl1-blue Stratagene Search Results


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    Stratagene escherichia coli strain xl1 blue
    pVII tag-modified helper phages are structurally and functionally identical to normal helper phages, and donate a defined phenotype to pVII. ( A ) Schematic illustration of the pV, pVII, pIX genomic junctions in the M13 genome framed by the unique BsrG I/ SnaB I RE sites. The three tag modifications, their site of insertion and physical characteristics are given. Isoelectric point (pI) as well as average charge (§) was computed using ProtParam ( http://ca.expasy.org/ ). ( B ) AviTag-pVII functionality as BirA substrate assessed by virion binding to magnetic SA beads and detection with an anti-M13 Ab (BirA enzyme activity provided by E. coli <t>XL1-Blue</t> or AVB100FmkII). ( C ) FLAG-pVII functionality as assessed by virion binding to immobilized anti-FLAG M2 and M5 mAbs and detection with anti-M13 Ab. ( D ) HIS 6 -pVII functionality assessed by virion binding to magnetic IMAC beads and detection with an anti-M13 Ab. Virion binding to the beads was done without ( gray bars ) or with ( black bar ) 300 mM imidazole in the binding buffer. ( E ) HIS 6 -pVII functionality assessed as in D, employing HIS 6 -pVII tagged pVIII phagemid display (pGALD8mFN, unpublished ) virions ( left part ). Specific IMAC bead capture efficiency assessed by titration of imidazole-eluted pVIII phagemid virions after bead capture, shown as percent of in-put (% recovery = out-put (cfu ampR )/in-put (cfu ampR )×100), right part .
    Escherichia Coli Strain Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 99/100, based on 653 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain xl1 blue/product/Stratagene
    Average 99 stars, based on 653 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain xl1 blue - by Bioz Stars, 2020-02
    99/100 stars
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    78
    Stratagene escherichia coli strain epicuran coli xl1 blue
    pVII tag-modified helper phages are structurally and functionally identical to normal helper phages, and donate a defined phenotype to pVII. ( A ) Schematic illustration of the pV, pVII, pIX genomic junctions in the M13 genome framed by the unique BsrG I/ SnaB I RE sites. The three tag modifications, their site of insertion and physical characteristics are given. Isoelectric point (pI) as well as average charge (§) was computed using ProtParam ( http://ca.expasy.org/ ). ( B ) AviTag-pVII functionality as BirA substrate assessed by virion binding to magnetic SA beads and detection with an anti-M13 Ab (BirA enzyme activity provided by E. coli <t>XL1-Blue</t> or AVB100FmkII). ( C ) FLAG-pVII functionality as assessed by virion binding to immobilized anti-FLAG M2 and M5 mAbs and detection with anti-M13 Ab. ( D ) HIS 6 -pVII functionality assessed by virion binding to magnetic IMAC beads and detection with an anti-M13 Ab. Virion binding to the beads was done without ( gray bars ) or with ( black bar ) 300 mM imidazole in the binding buffer. ( E ) HIS 6 -pVII functionality assessed as in D, employing HIS 6 -pVII tagged pVIII phagemid display (pGALD8mFN, unpublished ) virions ( left part ). Specific IMAC bead capture efficiency assessed by titration of imidazole-eluted pVIII phagemid virions after bead capture, shown as percent of in-put (% recovery = out-put (cfu ampR )/in-put (cfu ampR )×100), right part .
    Escherichia Coli Strain Epicuran Coli Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain epicuran coli xl1 blue/product/Stratagene
    Average 78 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain epicuran coli xl1 blue - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier



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    Stratagene escherichia coli strain xl1 blue reca1 enda1 gyra96 thi hsdr17 supe44 rela1 lac
    (A) Recombinant expression of GST-tagged BPS in E. coli XL 1-Blue <t>MRF and purification by glutathione-agarose affinity chromatography. The Coomassie-stained gel shows E. coli whole-cell extracts prior to induction (lane 1) and after induction (lane 2) and purified recombinant BPS fusion protein (lane 3). (B) Western blot analysis of whole-cell lysates of GBS strain Compton R (lane 1), E. coli XL 1-Blue MRF" harboring plasmid pGEX2T (lane 2), and E. coli XL 1-Blue MRF" harboring plasmid pSE4 (lane 3) using a polyclonal antiserum raised against purified recombinant GST-BPS fusion protein. (C) Western blot analysis of purified recombinant BPS protein as well as whole-cell extracts of GBS and E. coli using BPS antiserum. Lane 1, purified recombinant BPS; lane 2, Compton R; lane 3, 71-735 (serotype III/R1); lane 4, H4A-0126 (type Ia/R1, BPS); lane 5, 76-043 (type III/R4); lane 6, E. coli XL1-Blue expressing BPS; lane 7, E. coli XL1-Blue control. " width="250" height="auto" />
    Escherichia Coli Strain Xl1 Blue Reca1 Enda1 Gyra96 Thi Hsdr17 Supe44 Rela1 Lac, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain xl1 blue reca1 enda1 gyra96 thi hsdr17 supe44 rela1 lac/product/Stratagene
    Average 78 stars, based on 9 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain xl1 blue reca1 enda1 gyra96 thi hsdr17 supe44 rela1 lac - by Bioz Stars, 2020-02
    78/100 stars
      Buy from Supplier

    Image Search Results


    pVII tag-modified helper phages are structurally and functionally identical to normal helper phages, and donate a defined phenotype to pVII. ( A ) Schematic illustration of the pV, pVII, pIX genomic junctions in the M13 genome framed by the unique BsrG I/ SnaB I RE sites. The three tag modifications, their site of insertion and physical characteristics are given. Isoelectric point (pI) as well as average charge (§) was computed using ProtParam ( http://ca.expasy.org/ ). ( B ) AviTag-pVII functionality as BirA substrate assessed by virion binding to magnetic SA beads and detection with an anti-M13 Ab (BirA enzyme activity provided by E. coli XL1-Blue or AVB100FmkII). ( C ) FLAG-pVII functionality as assessed by virion binding to immobilized anti-FLAG M2 and M5 mAbs and detection with anti-M13 Ab. ( D ) HIS 6 -pVII functionality assessed by virion binding to magnetic IMAC beads and detection with an anti-M13 Ab. Virion binding to the beads was done without ( gray bars ) or with ( black bar ) 300 mM imidazole in the binding buffer. ( E ) HIS 6 -pVII functionality assessed as in D, employing HIS 6 -pVII tagged pVIII phagemid display (pGALD8mFN, unpublished ) virions ( left part ). Specific IMAC bead capture efficiency assessed by titration of imidazole-eluted pVIII phagemid virions after bead capture, shown as percent of in-put (% recovery = out-put (cfu ampR )/in-put (cfu ampR )×100), right part .

    Journal: PLoS ONE

    Article Title: Expanding the Versatility of Phage Display I: Efficient Display of Peptide-Tags on Protein VII of the Filamentous Phage

    doi: 10.1371/journal.pone.0014702

    Figure Lengend Snippet: pVII tag-modified helper phages are structurally and functionally identical to normal helper phages, and donate a defined phenotype to pVII. ( A ) Schematic illustration of the pV, pVII, pIX genomic junctions in the M13 genome framed by the unique BsrG I/ SnaB I RE sites. The three tag modifications, their site of insertion and physical characteristics are given. Isoelectric point (pI) as well as average charge (§) was computed using ProtParam ( http://ca.expasy.org/ ). ( B ) AviTag-pVII functionality as BirA substrate assessed by virion binding to magnetic SA beads and detection with an anti-M13 Ab (BirA enzyme activity provided by E. coli XL1-Blue or AVB100FmkII). ( C ) FLAG-pVII functionality as assessed by virion binding to immobilized anti-FLAG M2 and M5 mAbs and detection with anti-M13 Ab. ( D ) HIS 6 -pVII functionality assessed by virion binding to magnetic IMAC beads and detection with an anti-M13 Ab. Virion binding to the beads was done without ( gray bars ) or with ( black bar ) 300 mM imidazole in the binding buffer. ( E ) HIS 6 -pVII functionality assessed as in D, employing HIS 6 -pVII tagged pVIII phagemid display (pGALD8mFN, unpublished ) virions ( left part ). Specific IMAC bead capture efficiency assessed by titration of imidazole-eluted pVIII phagemid virions after bead capture, shown as percent of in-put (% recovery = out-put (cfu ampR )/in-put (cfu ampR )×100), right part .

    Article Snippet: The E. coli strains XL1-Blue (recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F proAB lacI q ZΔM15 Tn10 (Tetr )] and CJ236 (FΔ(HindIII)::cat (Tra+Pil+Camr )/ung-1 relA1 dut-1 thi-1 spoT1 mcrA ) were purchased from Stratagene (LaJolla, CA, USA) and New England Biolabs (Ipswich, MA, USA), respectively.

    Techniques: Modification, Binding Assay, Activity Assay, Titration

    (A) Recombinant expression of GST-tagged BPS in E. coli XL 1-Blue MRF

    Journal: Infection and Immunity

    Article Title: Molecular Analysis of Group B Protective Surface Protein, a New Cell Surface Protective Antigen of Group B Streptococci

    doi:

    Figure Lengend Snippet: (A) Recombinant expression of GST-tagged BPS in E. coli XL 1-Blue MRF" and purification by glutathione-agarose affinity chromatography. The Coomassie-stained gel shows E. coli whole-cell extracts prior to induction (lane 1) and after induction (lane 2) and purified recombinant BPS fusion protein (lane 3). (B) Western blot analysis of whole-cell lysates of GBS strain Compton R (lane 1), E. coli XL 1-Blue MRF" harboring plasmid pGEX2T (lane 2), and E. coli XL 1-Blue MRF" harboring plasmid pSE4 (lane 3) using a polyclonal antiserum raised against purified recombinant GST-BPS fusion protein. (C) Western blot analysis of purified recombinant BPS protein as well as whole-cell extracts of GBS and E. coli using BPS antiserum. Lane 1, purified recombinant BPS; lane 2, Compton R; lane 3, 71-735 (serotype III/R1); lane 4, H4A-0126 (type Ia/R1, BPS); lane 5, 76-043 (type III/R4); lane 6, E. coli XL1-Blue expressing BPS; lane 7, E. coli XL1-Blue control.

    Article Snippet: The Escherichia coli strains XL1-Blue MRF and XLOLR were obtained from a commercial source (Stratagene).

    Techniques: Recombinant, Expressing, Purification, Affinity Chromatography, Staining, Western Blot, Plasmid Preparation, IA