e. coli strains xl1-blue Stratagene Search Results


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  • 91
    Stratagene e coli strain xl1 blue
    SDS-PAGE analysis of the PhaP-rPA fusion protein. Lane M, protein marker; lane 1, control; lane 2, lysate of E. coli <t>XL1-Blue</t> (pHBS01/pSPAr); lane 3, PHB granules isolated from E. coli XL1-Blue (pHBS01/pSPAr).
    E Coli Strain Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 91/100, based on 605 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene e coli xl1 blue
    Immunoblot showing OmpL1 expression at different IPTG concentrations. A culture of E. coli <t>XL1-Blue</t> containing plasmid pMMB66-OmpL1 was grown to log phase, separated into four equal volumes, and treated with various concentrations of IPTG. Cells were incubated for an additional 3 h in a shaker incubator at 37°C, pelleted in a microcentrifuge, resuspended in final sample buffer, and analyzed by SDS-PAGE and immunoblotting. A companion SDS-polyacrylamide gel loaded with the same four samples was stained with Coomassie brilliant blue to verify that all four lanes contained comparable amounts of material (data not shown). The locations of molecular size standards are shown (in kilodaltons) on the left. Even though use of the tac promoter typically results in relatively high background expression levels, expression using plasmid pMMB66-OmpL1 was not detectable without IPTG induction.
    E Coli Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 1750 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Stratagene e coli k 12 strain xl1 blue
    Immunoblot showing OmpL1 expression at different IPTG concentrations. A culture of E. coli <t>XL1-Blue</t> containing plasmid pMMB66-OmpL1 was grown to log phase, separated into four equal volumes, and treated with various concentrations of IPTG. Cells were incubated for an additional 3 h in a shaker incubator at 37°C, pelleted in a microcentrifuge, resuspended in final sample buffer, and analyzed by SDS-PAGE and immunoblotting. A companion SDS-polyacrylamide gel loaded with the same four samples was stained with Coomassie brilliant blue to verify that all four lanes contained comparable amounts of material (data not shown). The locations of molecular size standards are shown (in kilodaltons) on the left. Even though use of the tac promoter typically results in relatively high background expression levels, expression using plasmid pMMB66-OmpL1 was not detectable without IPTG induction.
    E Coli K 12 Strain Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene escherichia coli
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Escherichia Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 1525 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    Stratagene plasmids e coli xl1 blue
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Plasmids E Coli Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Stratagene bacterial strains escherichia coli strain xl1 blue
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Bacterial Strains Escherichia Coli Strain Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Stratagene bacterial strains escherichia coli xl1 blue mrf
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Bacterial Strains Escherichia Coli Xl1 Blue Mrf, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    88
    Stratagene e coli strain xl1 blue reca1 enda1 gyra96 thi 1 hsdr17 supe44 rela1 lac
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    E Coli Strain Xl1 Blue Reca1 Enda1 Gyra96 Thi 1 Hsdr17 Supe44 Rela1 Lac, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Stratagene plasmids escherichia coli strain xl1 blue
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Plasmids Escherichia Coli Strain Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene escherichia coli k12 strain xl1 blue
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Escherichia Coli K12 Strain Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Stratagene plasmid dna e coli xl1 blue
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Plasmid Dna E Coli Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    85
    Stratagene recombinant bacterial strains escherichia coli xl1 blue
    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with <t>Escherichia</t> coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P
    Recombinant Bacterial Strains Escherichia Coli Xl1 Blue, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SDS-PAGE analysis of the PhaP-rPA fusion protein. Lane M, protein marker; lane 1, control; lane 2, lysate of E. coli XL1-Blue (pHBS01/pSPAr); lane 3, PHB granules isolated from E. coli XL1-Blue (pHBS01/pSPAr).

    Journal: Applied and Environmental Microbiology

    Article Title: Expression of Active Recombinant Human Tissue-Type Plasminogen Activator by Using In Vivo Polyhydroxybutyrate Granule Display ▿

    doi: 10.1128/AEM.01543-10

    Figure Lengend Snippet: SDS-PAGE analysis of the PhaP-rPA fusion protein. Lane M, protein marker; lane 1, control; lane 2, lysate of E. coli XL1-Blue (pHBS01/pSPAr); lane 3, PHB granules isolated from E. coli XL1-Blue (pHBS01/pSPAr).

    Article Snippet: E. coli strain XL1-Blue [ recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac F′ proAB lacI q Z ΔM15 Tn 10 (Tetr )] from Stratagene was used for recombinant protein expression.

    Techniques: SDS Page, Recombinase Polymerase Amplification, Marker, Isolation

    Cell growth and PHB accumulation of recombinant E. coli . The strains were cultivated in LB medium supplemented with 0.5% glucose. (A) Comparison of PHB content and cell mass in E. coli BL21/pHBS01 and XL1-Blue/pHBS01. (B) Fluorescence micrograph

    Journal: Applied and Environmental Microbiology

    Article Title: Expression of Active Recombinant Human Tissue-Type Plasminogen Activator by Using In Vivo Polyhydroxybutyrate Granule Display ▿

    doi: 10.1128/AEM.01543-10

    Figure Lengend Snippet: Cell growth and PHB accumulation of recombinant E. coli . The strains were cultivated in LB medium supplemented with 0.5% glucose. (A) Comparison of PHB content and cell mass in E. coli BL21/pHBS01 and XL1-Blue/pHBS01. (B) Fluorescence micrograph

    Article Snippet: E. coli strain XL1-Blue [ recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac F′ proAB lacI q Z ΔM15 Tn 10 (Tetr )] from Stratagene was used for recombinant protein expression.

    Techniques: Recombinant, Fluorescence

    Western blot analysis of the PhaP-rPA fusion protein. Lane M, protein marker; lane 1, control; lane 2, PHB granules isolated from E. coli XL1-Blue (pHBS01/pSPAr).

    Journal: Applied and Environmental Microbiology

    Article Title: Expression of Active Recombinant Human Tissue-Type Plasminogen Activator by Using In Vivo Polyhydroxybutyrate Granule Display ▿

    doi: 10.1128/AEM.01543-10

    Figure Lengend Snippet: Western blot analysis of the PhaP-rPA fusion protein. Lane M, protein marker; lane 1, control; lane 2, PHB granules isolated from E. coli XL1-Blue (pHBS01/pSPAr).

    Article Snippet: E. coli strain XL1-Blue [ recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac F′ proAB lacI q Z ΔM15 Tn 10 (Tetr )] from Stratagene was used for recombinant protein expression.

    Techniques: Western Blot, Recombinase Polymerase Amplification, Marker, Isolation

    Fibrin plate analysis of rPA activity. Equal amounts of the samples were spotted on the fibrin plate. (A) Cell debris from E. coli XL1-Blue (pSCP/pSPAr). (B) Isolated PHB granules from E. coli XL1-Blue (pHBS01/pSPAr).

    Journal: Applied and Environmental Microbiology

    Article Title: Expression of Active Recombinant Human Tissue-Type Plasminogen Activator by Using In Vivo Polyhydroxybutyrate Granule Display ▿

    doi: 10.1128/AEM.01543-10

    Figure Lengend Snippet: Fibrin plate analysis of rPA activity. Equal amounts of the samples were spotted on the fibrin plate. (A) Cell debris from E. coli XL1-Blue (pSCP/pSPAr). (B) Isolated PHB granules from E. coli XL1-Blue (pHBS01/pSPAr).

    Article Snippet: E. coli strain XL1-Blue [ recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac F′ proAB lacI q Z ΔM15 Tn 10 (Tetr )] from Stratagene was used for recombinant protein expression.

    Techniques: Recombinase Polymerase Amplification, Activity Assay, Isolation

    Growth of E. coli strains on (GlcNAc) 2 and cellobiose. E. coli strains XL1-Blue MR (wild type, •), Xm1.4 (transposon mutant, ▴), and Xm1.4:pES1 (transposon mutant harboring the 7.3-kb clone, ○) were tested for their ability to grow on (GlcNAc) 2 (solid lines) and cellobiose (dashed lines) as sole carbon sources. Minimal media (M9 salts) supplemented with 0.5 mM thiamine and 0.05% Casamino acids containing either 10 mM (GlcNAc) 2 or 10 mM cellobiose were inoculated using a 1:100 dilution of overnight cultures grown in LB. Growth was monitored over the indicated time course by using a Klett photoelectric colorimeter with a no. 54 green filter (550 nm). At the arrow, an aliquot of Xm1.4:pES1 cells [preinduced by growth to mid-logarithmic phase on (GlcNAc) 2 ] was harvested and washed three times with an equal volume of minimal medium and then resuspended in minimal medium containing 10 mM cellobiose.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Wild-type Escherichia coli grows on the chitin disaccharide, N,N?-diacetylchitobiose, by expressing the cel operon

    doi:

    Figure Lengend Snippet: Growth of E. coli strains on (GlcNAc) 2 and cellobiose. E. coli strains XL1-Blue MR (wild type, •), Xm1.4 (transposon mutant, ▴), and Xm1.4:pES1 (transposon mutant harboring the 7.3-kb clone, ○) were tested for their ability to grow on (GlcNAc) 2 (solid lines) and cellobiose (dashed lines) as sole carbon sources. Minimal media (M9 salts) supplemented with 0.5 mM thiamine and 0.05% Casamino acids containing either 10 mM (GlcNAc) 2 or 10 mM cellobiose were inoculated using a 1:100 dilution of overnight cultures grown in LB. Growth was monitored over the indicated time course by using a Klett photoelectric colorimeter with a no. 54 green filter (550 nm). At the arrow, an aliquot of Xm1.4:pES1 cells [preinduced by growth to mid-logarithmic phase on (GlcNAc) 2 ] was harvested and washed three times with an equal volume of minimal medium and then resuspended in minimal medium containing 10 mM cellobiose.

    Article Snippet: E. coli strain XL1-Blue MR was purchased from Stratagene, and E. coli strain LR-175 , Salmonella typhimurium strain LT2 , and other strains were obtained from the American Type Culture Collection.

    Techniques: Mutagenesis

    (A) Recombinant expression of GST-tagged BPS in E. coli XL 1-Blue MRF

    Journal: Infection and Immunity

    Article Title: Molecular Analysis of Group B Protective Surface Protein, a New Cell Surface Protective Antigen of Group B Streptococci

    doi:

    Figure Lengend Snippet: (A) Recombinant expression of GST-tagged BPS in E. coli XL 1-Blue MRF" and purification by glutathione-agarose affinity chromatography. The Coomassie-stained gel shows E. coli whole-cell extracts prior to induction (lane 1) and after induction (lane 2) and purified recombinant BPS fusion protein (lane 3). (B) Western blot analysis of whole-cell lysates of GBS strain Compton R (lane 1), E. coli XL 1-Blue MRF" harboring plasmid pGEX2T (lane 2), and E. coli XL 1-Blue MRF" harboring plasmid pSE4 (lane 3) using a polyclonal antiserum raised against purified recombinant GST-BPS fusion protein. (C) Western blot analysis of purified recombinant BPS protein as well as whole-cell extracts of GBS and E. coli using BPS antiserum. Lane 1, purified recombinant BPS; lane 2, Compton R; lane 3, 71-735 (serotype III/R1); lane 4, H4A-0126 (type Ia/R1, BPS); lane 5, 76-043 (type III/R4); lane 6, E. coli XL1-Blue expressing BPS; lane 7, E. coli XL1-Blue control.

    Article Snippet: The Escherichia coli strains XL1-Blue MRF and XLOLR were obtained from a commercial source (Stratagene).

    Techniques: Recombinant, Expressing, Purification, Affinity Chromatography, Staining, Western Blot, Plasmid Preparation, IA

    Immunoblot showing OmpL1 expression at different IPTG concentrations. A culture of E. coli XL1-Blue containing plasmid pMMB66-OmpL1 was grown to log phase, separated into four equal volumes, and treated with various concentrations of IPTG. Cells were incubated for an additional 3 h in a shaker incubator at 37°C, pelleted in a microcentrifuge, resuspended in final sample buffer, and analyzed by SDS-PAGE and immunoblotting. A companion SDS-polyacrylamide gel loaded with the same four samples was stained with Coomassie brilliant blue to verify that all four lanes contained comparable amounts of material (data not shown). The locations of molecular size standards are shown (in kilodaltons) on the left. Even though use of the tac promoter typically results in relatively high background expression levels, expression using plasmid pMMB66-OmpL1 was not detectable without IPTG induction.

    Journal: Infection and Immunity

    Article Title: Leptospiral Outer Membrane Proteins OmpL1 and LipL41 Exhibit Synergistic Immunoprotection

    doi:

    Figure Lengend Snippet: Immunoblot showing OmpL1 expression at different IPTG concentrations. A culture of E. coli XL1-Blue containing plasmid pMMB66-OmpL1 was grown to log phase, separated into four equal volumes, and treated with various concentrations of IPTG. Cells were incubated for an additional 3 h in a shaker incubator at 37°C, pelleted in a microcentrifuge, resuspended in final sample buffer, and analyzed by SDS-PAGE and immunoblotting. A companion SDS-polyacrylamide gel loaded with the same four samples was stained with Coomassie brilliant blue to verify that all four lanes contained comparable amounts of material (data not shown). The locations of molecular size standards are shown (in kilodaltons) on the left. Even though use of the tac promoter typically results in relatively high background expression levels, expression using plasmid pMMB66-OmpL1 was not detectable without IPTG induction.

    Article Snippet: Expression of OmpL1-M was studied in assays using a variety of different host strains, and E. coli XL1-Blue was found to produce the most consistent results.

    Techniques: Expressing, Plasmid Preparation, Incubation, SDS Page, Staining

    Coomassie brilliant blue-stained SDS-polyacrylamide gel showing material representative of E. coli membrane fractions used to immunize hamsters. Lanes: 1 and 2, E. coli XL1-Blue with plasmids pMMB66 and pMMB66-OmpL1, respectively; 3 and 4, E. coli JM109(DE3) with plasmids pET15b and pET15b-LipL41*, respectively. Locations of the OmpL1 doublet in lane 2 and of LipL41 in lane 4 are shown (arrowheads). Positions of molecular size markers (M) are given in kilodaltons.

    Journal: Infection and Immunity

    Article Title: Leptospiral Outer Membrane Proteins OmpL1 and LipL41 Exhibit Synergistic Immunoprotection

    doi:

    Figure Lengend Snippet: Coomassie brilliant blue-stained SDS-polyacrylamide gel showing material representative of E. coli membrane fractions used to immunize hamsters. Lanes: 1 and 2, E. coli XL1-Blue with plasmids pMMB66 and pMMB66-OmpL1, respectively; 3 and 4, E. coli JM109(DE3) with plasmids pET15b and pET15b-LipL41*, respectively. Locations of the OmpL1 doublet in lane 2 and of LipL41 in lane 4 are shown (arrowheads). Positions of molecular size markers (M) are given in kilodaltons.

    Article Snippet: Expression of OmpL1-M was studied in assays using a variety of different host strains, and E. coli XL1-Blue was found to produce the most consistent results.

    Techniques: Staining

    Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with Escherichia coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P

    Journal: Fish & Shellfish Immunology

    Article Title: Differential modulations of two glyceraldehyde 3-phosphate dehydrogenase mRNAs in response to bacterial and viral challenges in a marine teleost Oplegnathus fasciatus (Perciformes)

    doi: 10.1016/j.fsi.2008.07.007

    Figure Lengend Snippet: Real-time RT-PCR assays of Oplegnathus fasciatus GAPDH mRNA isoforms in response to bacterial challenges (A) and viral infection (B). Bacterial challenges were performed with Escherichia coli (EC), Edwardsiella tarda (ET), Vibrio anguillarum (VA) or Streptococcus iniae (SI), and tissue samples were obtained 48 h post-challenge for expression assays. On the other hand, viral challenge was conducted with rockbream iridovirus (RBIV) and expression assays were carried out 9 days post-challenge. Relative mRNA expressions of bacteria- or RBIV-injected groups to the PBS-injected control were determined by the comparative Ct method considering PCR efficiency based on the normalization against the expression of 18S rRNA in each sample. Fold increases (mean ± SDs based on triplicate assays) were represented in histograms (open bars for rbGAPDH1 and closed bars for rbGAPDH2). Significant differences from the control expression were noted by asterisks based on Student's t -tests ( P

    Article Snippet: Bacterial species tested were Escherichia coli (XL1 blue MRF′ strain; Stratagene), Edwardsiella tarda (Gram−; FSW910410), Vibrio anguillarum (Gram−; KFCC11377P) and Streptococcus iniae (Gram+; JSL0108).

    Techniques: Quantitative RT-PCR, Infection, Expressing, Injection, Polymerase Chain Reaction