e. coli dna polymerase Thermo Fisher Search Results


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  • 99
    Thermo Fisher total rna
    Total Rna, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 498778 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher e coli dna polymerase
    E Coli Dna Polymerase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 636 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher dna polymerase i
    Dna Polymerase I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 2890 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent top10 e coli cells
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    Thermo Fisher escherichia coli
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    Thermo Fisher ta cloning kit
    Ta Cloning Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 15413 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher competent e coli
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    Thermo Fisher e coli dna polymerase 1
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    Thermo Fisher e coli top10 cell
    Competition assays and G. mellonella killing models in HLCRMs. a Fitness of full-length mcr-1 , calalytically inactivated mcr-1 (E246A), and mcr-1 soluble domain. Fitness of blaTEM-1b as a negative control. b Relative fitness of wild-type mcr-1 -positive strains and their derivatives. c Relative fitness of mcr-1/ pBAD-positive E.coli <t>TOP10</t> strains and their derivatives. In all fitness figures, error bars represent the SD ( n = 6). The differences in fitness were tested using non-parametric Mann–Whitney test, * indicates 0.01
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    Thermo Fisher top10 escherichia coli
    DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into <t>TOP10</t> <t>Escherichia</t> coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).
    Top10 Escherichia Coli, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 89/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Competition assays and G. mellonella killing models in HLCRMs. a Fitness of full-length mcr-1 , calalytically inactivated mcr-1 (E246A), and mcr-1 soluble domain. Fitness of blaTEM-1b as a negative control. b Relative fitness of wild-type mcr-1 -positive strains and their derivatives. c Relative fitness of mcr-1/ pBAD-positive E.coli TOP10 strains and their derivatives. In all fitness figures, error bars represent the SD ( n = 6). The differences in fitness were tested using non-parametric Mann–Whitney test, * indicates 0.01

    Journal: Nature Communications

    Article Title: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms

    doi: 10.1038/s41467-017-02149-0

    Figure Lengend Snippet: Competition assays and G. mellonella killing models in HLCRMs. a Fitness of full-length mcr-1 , calalytically inactivated mcr-1 (E246A), and mcr-1 soluble domain. Fitness of blaTEM-1b as a negative control. b Relative fitness of wild-type mcr-1 -positive strains and their derivatives. c Relative fitness of mcr-1/ pBAD-positive E.coli TOP10 strains and their derivatives. In all fitness figures, error bars represent the SD ( n = 6). The differences in fitness were tested using non-parametric Mann–Whitney test, * indicates 0.01

    Article Snippet: All resultant plasmids were transformed into E. coli TOP10 cell (Invitrogen, UK), purified and its integrity confirmed by PCR, double restriction digestion and DNA sequencing.

    Techniques: Negative Control, MANN-WHITNEY

    TEM micrographs of untreated and treated E.coli . In a and b , TEM micrographs of untreated control cells ( E. coli TOP10 with pBAD minus mcr-1 , and E. coli TOP10 ( mcr-1 /pBAD) without l -arabinose induction, respectively); both cells are intact with a well-defined inner and outer membrane, and showed a highly homogeneous electron density in cytoplasm region ( d and e ). c TEM micrographs of mcr-1 overproducing cells; the damaging outer membrane and some completely lysed cells were observed ( f )

    Journal: Nature Communications

    Article Title: Balancing mcr-1 expression and bacterial survival is a delicate equilibrium between essential cellular defence mechanisms

    doi: 10.1038/s41467-017-02149-0

    Figure Lengend Snippet: TEM micrographs of untreated and treated E.coli . In a and b , TEM micrographs of untreated control cells ( E. coli TOP10 with pBAD minus mcr-1 , and E. coli TOP10 ( mcr-1 /pBAD) without l -arabinose induction, respectively); both cells are intact with a well-defined inner and outer membrane, and showed a highly homogeneous electron density in cytoplasm region ( d and e ). c TEM micrographs of mcr-1 overproducing cells; the damaging outer membrane and some completely lysed cells were observed ( f )

    Article Snippet: All resultant plasmids were transformed into E. coli TOP10 cell (Invitrogen, UK), purified and its integrity confirmed by PCR, double restriction digestion and DNA sequencing.

    Techniques: Transmission Electron Microscopy

    DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into TOP10 Escherichia coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).

    Journal: Clinical and Experimental Immunology

    Article Title: Tissue‐resident memory T cells are epigenetically cytotoxic with signs of exhaustion in human urinary bladder cancer

    doi: 10.1111/cei.13183

    Figure Lengend Snippet: DNA methylation in the enhancer region regulates perforin transcription. (a) Promoter region sequence containing enhancer in the PRF1 gene was aligned between human and mouse using the VISTA tool. The conservation degree is indicated by the peaks, with coloured peaks (blue for exon and light red for the non‐coding sequence) indicating > 70% conservation. Ten cytosine–phosphate–guanine sites (CpGs) in the enhancer are displayed upstream from the transcription start site (TSS). (b) Sorted CD8 + T cells from healthy donors were cultured initially in the presence of 200 IU/ml recombinant human interleukin (IL)‐2, 5 μg/ml plate‐coated anti‐CD3 and 1 μg/ml anti‐CD28 stimulating antibodies in vitro . 5‐Azacytidine (5‐aza) was added to the culture at a final concentration of 2·5 μM/ml for 48 h, followed by culture medium replacement and incubation for another 48 h. Flow cytometry data demonstrating perforin expression pre‐ and post‐treatment are shown. (c) Perforin – and perforin + CD8 + T cells were sorted. DNA was extracted and bisulfite‐converted from each cell subset, TA‐cloned to pCR4‐TOPO vector and transformed into TOP10 Escherichia coli . Colonies were picked from each cell subset and their DNA was sequenced to measure DNA methylation status of the 10 CpGs in the enhancer. The data display rows represent individually sequenced clone. (d) The bar graphs display total DNA methylation percentage in each CpG from clones in (c).

    Article Snippet: Next, the PCR amplicons were TA‐cloned into pCR4‐TOPO vector (ThermoFisher) and transformed into TOP10 Escherichia coli (ThermoFisher) by heat shock, according to the manufacturer’s protocol.

    Techniques: DNA Methylation Assay, Sequencing, Cell Culture, Recombinant, In Vitro, Concentration Assay, Incubation, Flow Cytometry, Cytometry, Expressing, Clone Assay, Plasmid Preparation, Transformation Assay