e. coli bl21-de3 Stratagene Search Results


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  • 99
    Millipore bl21
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    Stratagene e coli bl21 de3 gold stratagene
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    Stratagene e coli bl21 de3 ril stratagene cells
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    Stratagene e coli bl21
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
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    Stratagene e coli bl21 de3
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
    E Coli Bl21 De3, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 867 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene competent e coli bl21
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
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    88
    Stratagene recombinant e coli bl21
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
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    Stratagene virus strains e coli bl21 gold de3 competent cells stratagene
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
    Virus Strains E Coli Bl21 Gold De3 Competent Cells Stratagene, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene circular dichroism e coli bl21
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
    Circular Dichroism E Coli Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene expression host e coli bl21
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
    Expression Host E Coli Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene strain escherichia coli bl21 de3
    SDS-PAGE analysis of purified protein recombinant SllB in E. coli <t>BL21</t> cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.
    Strain Escherichia Coli Bl21 De3, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli bl21 codonplus de3 ril
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    E Coli Bl21 Codonplus De3 Ril, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 373 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene bl21 de3 plyss e coli
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    Bl21 De3 Plyss E Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene escherichia coli bl21 de3 ril
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    Escherichia Coli Bl21 De3 Ril, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 137 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli bl21 codonplus de3 riltm
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    E Coli Bl21 Codonplus De3 Riltm, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli bl21 de3 ripl
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    E Coli Bl21 De3 Ripl, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene escherichia coli bl21 codonplus de3 ripl
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    Escherichia Coli Bl21 Codonplus De3 Ripl, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 69 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli epicurian bl21 codonplus de3 ril
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    E Coli Epicurian Bl21 Codonplus De3 Ril, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene escherichia coli bl21 codonplus de3 ril cells
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    Escherichia Coli Bl21 Codonplus De3 Ril Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 89/100, based on 490 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli bl21 codonplus de3 rp
    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli <t>BL21-Codonplus-(DE3)-RIL</t> carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.
    E Coli Bl21 Codonplus De3 Rp, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 64 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    SDS-PAGE analysis of purified protein recombinant SllB in E. coli BL21 cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.

    Journal: International Journal of Molecular Medicine

    Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification

    doi: 10.3892/ijmm.2012.890

    Figure Lengend Snippet: SDS-PAGE analysis of purified protein recombinant SllB in E. coli BL21 cells. Lane M: Takara Protein Marker; lane 1, SDS-PAGE analysis of the recombinant S-layer protein before purification; lane 2, SDS-PAGE analysis of the purified recombinant protein.

    Article Snippet: 2.0 (Takara, no. DV801A), from which 0.5 μl was used to transform 100 μl of E. coli BL21 (DE3, Stratagene).

    Techniques: SDS Page, Purification, Recombinant, Marker

    A transmission electron microscopic observation on the E. coli BL21 with recombinant protein. (A) Normal E. coli BL21 was treated as control. (B) The E. coli BL21 cells recombinant S-layer protein. (C) Crystal lattice structures on surface of the E. coli BL21 cells recombinant S-layer protein.

    Journal: International Journal of Molecular Medicine

    Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification

    doi: 10.3892/ijmm.2012.890

    Figure Lengend Snippet: A transmission electron microscopic observation on the E. coli BL21 with recombinant protein. (A) Normal E. coli BL21 was treated as control. (B) The E. coli BL21 cells recombinant S-layer protein. (C) Crystal lattice structures on surface of the E. coli BL21 cells recombinant S-layer protein.

    Article Snippet: 2.0 (Takara, no. DV801A), from which 0.5 μl was used to transform 100 μl of E. coli BL21 (DE3, Stratagene).

    Techniques: Transmission Assay, Recombinant

    Expression of sllB in E. coli BL21 cells. (A) SDS-PAGE analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; lane M represent Takara Protein Marker (Broad). (B) Western blot analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; M, Takara Protein Marker (Broad). Lane M1, Precision plus protein standards; lane M2, perfect protein marker. Note the band pointed with arrows is the recombinant S-layer protein.

    Journal: International Journal of Molecular Medicine

    Article Title: Cloning of the surface layer gene sllB from Bacillus sphaericus ATCC 14577 and its heterologous expression and purification

    doi: 10.3892/ijmm.2012.890

    Figure Lengend Snippet: Expression of sllB in E. coli BL21 cells. (A) SDS-PAGE analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; lane M represent Takara Protein Marker (Broad). (B) Western blot analysis of rSllB protein; lane 1, the whole cell lysate of E. coli BL21 cells containing pET28a(+); lane 2, the supernatant of cells containing pET28a(+); lane 3, the pellet of cells containing pET28a(+); lane 4, the whole cell lysate of E. coli BL21 cells containing pET28a(+)- sllB ; lane 5, the supernatant of cell containing pET28a(+)- sllB ; lane 6, the pellet of cells containing pET28a(+)- sllB ; M, Takara Protein Marker (Broad). Lane M1, Precision plus protein standards; lane M2, perfect protein marker. Note the band pointed with arrows is the recombinant S-layer protein.

    Article Snippet: 2.0 (Takara, no. DV801A), from which 0.5 μl was used to transform 100 μl of E. coli BL21 (DE3, Stratagene).

    Techniques: Expressing, SDS Page, Marker, Western Blot, Recombinant

    Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli BL21-Codonplus-(DE3)-RIL carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.

    Journal: Journal of Bacteriology

    Article Title: The Alternative Sigma Factor ?B of Bacillus cereus: Response to Stress and Role in Heat Adaptation

    doi: 10.1128/JB.186.2.316-325.2004

    Figure Lengend Snippet: Overproduction and purification of σ B . (Left panel) SDS-PAGE of protein extracts from E. coli BL21-Codonplus-(DE3)-RIL carrying either pET28-b or pMT01. (Right panel) Immunodetection of σ B with anti-σ B antiserum. Ten micrograms of protein was loaded for each sample. Lane 1, crude protein extract from E. coli carrying pET28-b; lane 2, crude protein extract from E. coli carrying pMT01; lane 3, crude protein extract from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 4, inclusion bodies isolated from E. coli carrying pMT01 after induction with 1 mM IPTG for 2 h; lane 5, representative fraction of purified σ B after elution from an Ni 2+ affinity column. The arrow indicates the position of the overproduced σ B protein.

    Article Snippet: E. coli BL21-Codonplus-(DE3)-RIL (Stratagene, La Jolla, Calif.) was used as the host for SigB overproduction.

    Techniques: Purification, SDS Page, Immunodetection, Isolation, Affinity Column