e-cadherin Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Millipore n cadherin
    EVI1 knockdown in epithelial-like COLO205 colon cancer cells induces EMT a Control siRNA and siRNA EVI1 transfected COLO205 cell images after invasion are shown after 1 day (upper panel) and 9 days (lower panel). b The protein expression of EVI1, SLUG, <t>E-CADHERIN,</t> and N-CADHERIN in the EMT induced COLO205 cells are shown. ACTIN was used as a loading control. c RQ-PCR analysis of EVI1 and EMT-related genes in the EMT induced COLO205 cells.
    N Cadherin, supplied by Millipore, used in various techniques. Bioz Stars score: 95/100, based on 827 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin/product/Millipore
    Average 95 stars, based on 827 article reviews
    Price from $9.99 to $1999.99
    n cadherin - by Bioz Stars, 2020-08
    95/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc e cadherin e cadherin
    Expression of FBP1 was suppressed during Snail-induced EMT. a The expression of Snail in HCC cell lines; Snail was highest in MHCC-97H but lowest in SMMC-7721. b Quantitative real-time PCR analysis showed significantly higher expression of Snail in SMMC-7721-Snail than control SMMC-7721 cells. c Transwell migration assay showed Snail overexpression significantly promoted SMMC-7721 cell migration. d CCK8 assay showed Snail overexpression did not significantly affect SMMC-7721 cell proliferation. e Quantitative real-time PCR analysis showed Snail overexpression significantly suppressed <t>E-cadherin</t> in SMMC-7721 cells. f Quantitative real-time PCR analysis showed Snail overexpression significantly induced Vimentin in SMMC-7721 cells. g Quantitative real-time PCR analysis showed Snail overexpression significantly inhibited FBP1 in SMMC-7721 cells. h Western blot analysis showed Snail overexpression induced Vimentin but suppressed E-cadherin and FBP1 protein in SMMC-7721. * P
    E Cadherin E Cadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 746 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin e cadherin/product/Cell Signaling Technology Inc
    Average 99 stars, based on 746 article reviews
    Price from $9.99 to $1999.99
    e cadherin e cadherin - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    88
    Cell Signaling Technology Inc anti ecadherin
    Expression of FBP1 was suppressed during Snail-induced EMT. a The expression of Snail in HCC cell lines; Snail was highest in MHCC-97H but lowest in SMMC-7721. b Quantitative real-time PCR analysis showed significantly higher expression of Snail in SMMC-7721-Snail than control SMMC-7721 cells. c Transwell migration assay showed Snail overexpression significantly promoted SMMC-7721 cell migration. d CCK8 assay showed Snail overexpression did not significantly affect SMMC-7721 cell proliferation. e Quantitative real-time PCR analysis showed Snail overexpression significantly suppressed <t>E-cadherin</t> in SMMC-7721 cells. f Quantitative real-time PCR analysis showed Snail overexpression significantly induced Vimentin in SMMC-7721 cells. g Quantitative real-time PCR analysis showed Snail overexpression significantly inhibited FBP1 in SMMC-7721 cells. h Western blot analysis showed Snail overexpression induced Vimentin but suppressed E-cadherin and FBP1 protein in SMMC-7721. * P
    Anti Ecadherin, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 88/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti ecadherin/product/Cell Signaling Technology Inc
    Average 88 stars, based on 19 article reviews
    Price from $9.99 to $1999.99
    anti ecadherin - by Bioz Stars, 2020-08
    88/100 stars
      Buy from Supplier

    99
    Cell Signaling Technology Inc n cadherin antibodies
    c‐Src inhibitor could not prevent epithelial‐mesenchymal transition ( EMT ) after knockdown of vimentin. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. Then, they were treated with different concentrations of PP 2 for 48 h to detect A, invasion and B, cell migration. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. C, Next, cells were treated with different concentrations of PP 2 for 24 h to measure wound healing. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. Next, cells were treated with different concentrations of PP 2 for 48 h. D, Cell lysates were harvested for immunoblotting with specific antibodies against vimentin, E‐cadherin, <t>N‐cadherin,</t> and Snail. ** P
    N Cadherin Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin antibodies/product/Cell Signaling Technology Inc
    Average 99 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    n cadherin antibodies - by Bioz Stars, 2020-08
    99/100 stars
      Buy from Supplier

    92
    Abcam e cadherin
    APE 1 promotes the metastatic potential of xenograft lung cancer cells in nude mice. One million HCC 827/ IR cells were inoculated subcutaneously into nude mice which were then treated with icotinib at 0 mg/kg, 70 mg/kg alone, and 70 mg/kg together with E3330 at 25 mg/kg (n = 6 mice or 12 xenografts/group). Xenograft growth was measured in two dimensions, and volume was recorded (tumor size (mm 3 ) = (maximum diameter ×minimum diameter 2 )/2) (A). After daily treatment for 12 d, the nude mice were sacrificed, and histologically intact xenografts were collected. The expression of APE 1, <t>E‐cadherin,</t> and vimentin in the xenograft extracts was detected by Western blot (B). EMT analyses of tissue slides were performed via IHC staining of E‐cadherin and vimentin (C). The mean values of the 12 xenografts of each group are shown as the mean ± SD , * Statistically significant difference when compared with the control group ( P
    E Cadherin, supplied by Abcam, used in various techniques. Bioz Stars score: 92/100, based on 6901 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Abcam
    Average 92 stars, based on 6901 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Agilent technologies e cadherin
    Representative example of immunopositivity grade 3 for <t>E-cadherin</t> (original magnification x400).
    E Cadherin, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 92/100, based on 1187 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Agilent technologies
    Average 92 stars, based on 1187 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Becton Dickinson e cadherin
    SNHG20 knockdown regulated several genes related to proliferation and metastasis ( A , B ) The mRNA level of P21, Cyclin D1, Vimentin, and <t>E-cadherin</t> was detected by qRT-PCR in A2780 and CAOV-3 cells transfecting with si-SNHG20-1 or -2 (* P
    E Cadherin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 94/100, based on 9399 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Becton Dickinson
    Average 94 stars, based on 9399 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    BioLegend e cadherin
    IL-33 decreases the expression of integrin α4β1 and CD62L on JEG3 cells. Following stimulation with 1 ng/ml rhIL-33 for 48 h, the adhesion and invasion-related molecules (integrins, <t>E-cadherin,</t> CD62L and CD44) on JEG3 cells were analyzed by flow cytometry, with the vehicle as the negative control. (A) Quantification of flow cytometry data. (B) Scatter plots of flow cytometry data. The data are expressed as the mean ± standard error. ***P
    E Cadherin, supplied by BioLegend, used in various techniques. Bioz Stars score: 92/100, based on 139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/BioLegend
    Average 92 stars, based on 139 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Santa Cruz Biotechnology e cadherin
    Effects of NF-κB specific inhibitor BAY-11-7082 on migration and EMT of HLEC-h3 cells. ( A ) Effects of BAY-11-7082 treatment on NF-κB activity in EMT cell model. ( B ) Effects of BAY-11-7082 treatment on cell migration. ( C ) Effects of BAY-11-7082 treatment on <t>E-cadherin</t> expression. ( D ) Effects of BAY-11-7082 treatment on α-SMA expression. Control group was TGF-β-induced transdifferentiated cells. Each experiment was independently repeated 3 times. ** Compared with control group, p
    E Cadherin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 7703 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Santa Cruz Biotechnology
    Average 92 stars, based on 7703 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    R&D Systems n cadherin
    <t>N-cadherin</t> determines the site of first neurite growth and subsequently recruits Golgi and centrosome. ( A ) Examples of neurons immunolabelled with anti-βIII-tubulin developing on coverslips coated with alternating stripes of the indicated substrate and PLL. Quantification of neurons with the soma on the interface of the two substrates shows that neurons grow the first neurite preferentially at the N-cadherin facing pole (experimental n =4). Asymmetric contact with either laminin ( n =4) or Tenascin C ( n =3) does not trigger outgrowth of the first neurite (⩾9 neurons/experiment). ( B ) Examples of centrosome position in hippocampal neurons immunolabelled with a neuron-specific antibody (anti-βIII-tubulin) and with an antibody recognizing the centrosome (anti-γ-tubulin) with their soma on the PLL/N-cadherin interface. The centrosome position of neurons was quantified at different developmental stages (round neurons n =5, one neurite n =7, stage 2 neurons n =9, ⩾8 cells/experiment). The centrosome is recruited towards the N-cadherin substrate. ( A , B ) t -Test, * P
    N Cadherin, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin/product/R&D Systems
    Average 92 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    n cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    94
    Cloud-Clone e cadherin
    Immunofluorescent profile of BST2 and <t>E-cadherin</t> in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (20,000/well) were seeded on the coverslips in 24-well cell culture plates and cultured overnight at 37°C in 5% CO 2 and 70% relative humidity. The next day, cells were treated with and without IFN-γ for 24 h followed by immunolocalization of BST2, E-cadherin, and actin as described in Materials and Methods . Panel a shows the expression of actin (red) and BST2 (green) in control and IFN-γ-treated HTR-8/SVneo cells. Panel b shows the expression of actin (red) and E-cadherin (green) in control and IFN-γ-treated cells. The nuclei were stained using Hoechst nuclear staining dye. Scale bar shows 20 μm.
    E Cadherin, supplied by Cloud-Clone, used in various techniques. Bioz Stars score: 94/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Cloud-Clone
    Average 94 stars, based on 14 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    94/100 stars
      Buy from Supplier

    92
    Epitomics n cadherin
    Defining characteristics of (A-F) epithelial and (G-L) epithelial-mesenchymal transition (EMT) tumors. (A) Large blood vessels and solid nests of tumor cells separated by connective tissue are observed in the microscopic H E sections. (G) The EMT tumor architecture is characterized by a heterogeneous population of cells, including loosely packed, elongated (spindle) tumor cells (B-F, H-L, M). During EMT there is a significant reduction in <t>E-cadherin,</t> CK8/18 and CK19 expression, with a concomitant upregulation of vimentin. (N) Without ultrasound, the EMT phenotype is also associated with lower liposomal accumulation than epithelial tumors, although the rate of accumulation was higher for EMT. (*P
    N Cadherin, supplied by Epitomics, used in various techniques. Bioz Stars score: 92/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin/product/Epitomics
    Average 92 stars, based on 133 article reviews
    Price from $9.99 to $1999.99
    n cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Guangzhou RiboBio e cadherin
    Slit2/Robo1 signaling activates the Src-mediated inhibition of <t>E-cadherin</t> IHC analysis of the expression of total Src and pSrc (Tyr416) in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (A). Inactivation of Slit2/Robo1 significantly reduced the expression of pSrc (Tyr 416) in SW620 and SW480 cells, and activation of Slit2/Robo1 signaling through overexpressing Slit2 or Robo1 expression promotes pSrc (Tyr 416) expression in HCT-116 cells (B). IHC analysis of the expression of E-cadherin in the tumor tissues of the Apc Min/+ and Apc Min/+ ; Slit2 mice (C). Inactivation of Src signaling significantly enhances the expression of E-cadherin but inhibits the expression of β-cateninin in SW620 and SW480 cells (D). Src inactivation in SW620 and SW480 cells also led to a reduced nuclear translocation of β-catenin (E). Inhibition of E-cadherin expression through siRNA technique could promote the expression of β-cateninin and vimentin in SW620 and SW480 cells (F). IHC analysis of the expression of vimentin in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (G). The data in IHC staining are representative of 11 mice per group (All mice were 24-week-old). The results of IHC were determined using IPP software, and the data were expressed as the mean ±S.D. *: P
    E Cadherin, supplied by Guangzhou RiboBio, used in various techniques. Bioz Stars score: 92/100, based on 26 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Guangzhou RiboBio
    Average 92 stars, based on 26 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Leica Biosystems e cadherin
    Pooled transcript expression corrected to β actin micro-dissected archival FFPE diagnostic biopsies of men treated by EBRT or PADT and stratified by early biochemical relapse or no-relapse. A . Expression of MSMB B . Expression of E <t>Cadherin</t> (*p = 0.02, **p
    E Cadherin, supplied by Leica Biosystems, used in various techniques. Bioz Stars score: 92/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e cadherin/product/Leica Biosystems
    Average 92 stars, based on 45 article reviews
    Price from $9.99 to $1999.99
    e cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    92
    Roche n cadherin
    <t>E-cadherin</t> is only expressed in ameloblasts capable of expressing p120. In the same K14-Cre p120-cKO mosaic incisor shown in Figure 8 , E-cadherin (top) and p120 catenin (bottom) was immunolocalized in adjacent cross-sections. E-cadherin is expressed exclusively in normal appearing ameloblasts (brackets) that also express p120. However, in flattened, malformed ameloblasts where p120 was ablated, immunostaining for E-cadherin was not detectable. EO, enamel organ; PO, pulp organ.
    N Cadherin, supplied by Roche, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/n cadherin/product/Roche
    Average 92 stars, based on 24 article reviews
    Price from $9.99 to $1999.99
    n cadherin - by Bioz Stars, 2020-08
    92/100 stars
      Buy from Supplier

    Image Search Results


    EVI1 knockdown in epithelial-like COLO205 colon cancer cells induces EMT a Control siRNA and siRNA EVI1 transfected COLO205 cell images after invasion are shown after 1 day (upper panel) and 9 days (lower panel). b The protein expression of EVI1, SLUG, E-CADHERIN, and N-CADHERIN in the EMT induced COLO205 cells are shown. ACTIN was used as a loading control. c RQ-PCR analysis of EVI1 and EMT-related genes in the EMT induced COLO205 cells.

    Journal: Cell Death & Disease

    Article Title: Ecotropic viral integration site 1 promotes metastasis independent of epithelial mesenchymal transition in colon cancer cells

    doi: 10.1038/s41419-017-0036-1

    Figure Lengend Snippet: EVI1 knockdown in epithelial-like COLO205 colon cancer cells induces EMT a Control siRNA and siRNA EVI1 transfected COLO205 cell images after invasion are shown after 1 day (upper panel) and 9 days (lower panel). b The protein expression of EVI1, SLUG, E-CADHERIN, and N-CADHERIN in the EMT induced COLO205 cells are shown. ACTIN was used as a loading control. c RQ-PCR analysis of EVI1 and EMT-related genes in the EMT induced COLO205 cells.

    Article Snippet: N-CADHERIN (EMD Millipore, USA), SLUG (mouse polyclonal) and HRP-conjugated rabbit/mouse secondary antibodies (Santa Cruz Biotechnology, USA) were used.

    Techniques: Transfection, Expressing, Polymerase Chain Reaction

    EVI1 expression correlates with E-CADHERIN, N-CADHERIN, and CD44 expression in colon cancer patient samples a A positive correlation between the levels of expression of EVI1 and E-CADHERIN was observed for stage IV samples ( n = 61). b A negative correlation between the levels of expression of EVI1 and N-CADHERIN was observed for stage IV samples ( n = 61). c A positive correlation between the levels of expression of EVI1 and CD44 was observed for stage IV samples that showed both EVI1 + /CD44 + samples ( n = 17). d A positive correlation between the levels of expression of EVI1 and CD44 was observed for stage IV samples that showed both EVI1 − /CD44 − samples ( n = 18).

    Journal: Cell Death & Disease

    Article Title: Ecotropic viral integration site 1 promotes metastasis independent of epithelial mesenchymal transition in colon cancer cells

    doi: 10.1038/s41419-017-0036-1

    Figure Lengend Snippet: EVI1 expression correlates with E-CADHERIN, N-CADHERIN, and CD44 expression in colon cancer patient samples a A positive correlation between the levels of expression of EVI1 and E-CADHERIN was observed for stage IV samples ( n = 61). b A negative correlation between the levels of expression of EVI1 and N-CADHERIN was observed for stage IV samples ( n = 61). c A positive correlation between the levels of expression of EVI1 and CD44 was observed for stage IV samples that showed both EVI1 + /CD44 + samples ( n = 17). d A positive correlation between the levels of expression of EVI1 and CD44 was observed for stage IV samples that showed both EVI1 − /CD44 − samples ( n = 18).

    Article Snippet: N-CADHERIN (EMD Millipore, USA), SLUG (mouse polyclonal) and HRP-conjugated rabbit/mouse secondary antibodies (Santa Cruz Biotechnology, USA) were used.

    Techniques: Expressing

    Expression of FBP1 was suppressed during Snail-induced EMT. a The expression of Snail in HCC cell lines; Snail was highest in MHCC-97H but lowest in SMMC-7721. b Quantitative real-time PCR analysis showed significantly higher expression of Snail in SMMC-7721-Snail than control SMMC-7721 cells. c Transwell migration assay showed Snail overexpression significantly promoted SMMC-7721 cell migration. d CCK8 assay showed Snail overexpression did not significantly affect SMMC-7721 cell proliferation. e Quantitative real-time PCR analysis showed Snail overexpression significantly suppressed E-cadherin in SMMC-7721 cells. f Quantitative real-time PCR analysis showed Snail overexpression significantly induced Vimentin in SMMC-7721 cells. g Quantitative real-time PCR analysis showed Snail overexpression significantly inhibited FBP1 in SMMC-7721 cells. h Western blot analysis showed Snail overexpression induced Vimentin but suppressed E-cadherin and FBP1 protein in SMMC-7721. * P

    Journal: Cell Death & Disease

    Article Title: Restoration of FBP1 suppressed Snail-induced epithelial to mesenchymal transition in hepatocellular carcinoma

    doi: 10.1038/s41419-018-1165-x

    Figure Lengend Snippet: Expression of FBP1 was suppressed during Snail-induced EMT. a The expression of Snail in HCC cell lines; Snail was highest in MHCC-97H but lowest in SMMC-7721. b Quantitative real-time PCR analysis showed significantly higher expression of Snail in SMMC-7721-Snail than control SMMC-7721 cells. c Transwell migration assay showed Snail overexpression significantly promoted SMMC-7721 cell migration. d CCK8 assay showed Snail overexpression did not significantly affect SMMC-7721 cell proliferation. e Quantitative real-time PCR analysis showed Snail overexpression significantly suppressed E-cadherin in SMMC-7721 cells. f Quantitative real-time PCR analysis showed Snail overexpression significantly induced Vimentin in SMMC-7721 cells. g Quantitative real-time PCR analysis showed Snail overexpression significantly inhibited FBP1 in SMMC-7721 cells. h Western blot analysis showed Snail overexpression induced Vimentin but suppressed E-cadherin and FBP1 protein in SMMC-7721. * P

    Article Snippet: Snail overexpression induced Vimentin but suppressed E-cadherin, indicating the induction of EMT (Fig. ).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Transwell Migration Assay, Over Expression, Migration, CCK-8 Assay, Western Blot

    Expression of FBP1 and EMT markers in five HCC cell lines. a Quantitative real-time PCR analysis of FBP1 in MHCC-97H, MHCC-97L, HepG2, Hep3B and SMMC-7721 cells. b Western blot analysis of FBP1, E-cadherin, Vimentin and Snail in MHCC-97H, MHCC-97L, HepG2, Hep3B and SMMC-7721 cells. All data are based on three independent repeats. MW molecular weight

    Journal: Cell Death & Disease

    Article Title: Restoration of FBP1 suppressed Snail-induced epithelial to mesenchymal transition in hepatocellular carcinoma

    doi: 10.1038/s41419-018-1165-x

    Figure Lengend Snippet: Expression of FBP1 and EMT markers in five HCC cell lines. a Quantitative real-time PCR analysis of FBP1 in MHCC-97H, MHCC-97L, HepG2, Hep3B and SMMC-7721 cells. b Western blot analysis of FBP1, E-cadherin, Vimentin and Snail in MHCC-97H, MHCC-97L, HepG2, Hep3B and SMMC-7721 cells. All data are based on three independent repeats. MW molecular weight

    Article Snippet: Snail overexpression induced Vimentin but suppressed E-cadherin, indicating the induction of EMT (Fig. ).

    Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot, Molecular Weight

    Ectopic FBP1 suppressed Snail-induced EMT and tumour growth in SMMC-7721 cells. a Western blot analysis showed expression changes of E-cadherin and Vimentin induced by Snail expression were hindered by forced expression of FBP1 in SMMC-7721 cells. Ectopic FBP1 expression did not significantly affect Snail, Slug, ZEB1 and Twist1 expression. b Immunofluorescence assay showed SMMC-7721-snail-FBP1 cells expressed higher E-cadherin but lower Vimentin levels and appeared more like epithelial cells than SMMC-7721-Snail cells. The magnifications used were ×200. c Transwell migration analyses showed FBP1 expression significantly inhibited cell migration induced by Snail overexpression in SMMC-7721 cells. d Representative images of day 42 tumours in mice transplanted with SMMC-7721, SMMC-7721-Snail and SMMC-7721-Snail-FBP1. e , f The mean tumour diameters and weights in each group are shown. g The representative images of Snail, FBP1 and E-cadherin expression in transplanted tumours. The magnifications used were ×200. * P

    Journal: Cell Death & Disease

    Article Title: Restoration of FBP1 suppressed Snail-induced epithelial to mesenchymal transition in hepatocellular carcinoma

    doi: 10.1038/s41419-018-1165-x

    Figure Lengend Snippet: Ectopic FBP1 suppressed Snail-induced EMT and tumour growth in SMMC-7721 cells. a Western blot analysis showed expression changes of E-cadherin and Vimentin induced by Snail expression were hindered by forced expression of FBP1 in SMMC-7721 cells. Ectopic FBP1 expression did not significantly affect Snail, Slug, ZEB1 and Twist1 expression. b Immunofluorescence assay showed SMMC-7721-snail-FBP1 cells expressed higher E-cadherin but lower Vimentin levels and appeared more like epithelial cells than SMMC-7721-Snail cells. The magnifications used were ×200. c Transwell migration analyses showed FBP1 expression significantly inhibited cell migration induced by Snail overexpression in SMMC-7721 cells. d Representative images of day 42 tumours in mice transplanted with SMMC-7721, SMMC-7721-Snail and SMMC-7721-Snail-FBP1. e , f The mean tumour diameters and weights in each group are shown. g The representative images of Snail, FBP1 and E-cadherin expression in transplanted tumours. The magnifications used were ×200. * P

    Article Snippet: Snail overexpression induced Vimentin but suppressed E-cadherin, indicating the induction of EMT (Fig. ).

    Techniques: Western Blot, Expressing, Immunofluorescence, Migration, Over Expression, Mouse Assay

    Extracellular matrix (ECM) architecture influences cell morphology and intracellular stiffness of HNSCC cell lines (FaDu, CAL‐27, SAS and OEC‐M1). A, Western blot of E‐cadherin and vimentin in four head and neck cancer cell lines FaDu, CAL‐27, SAS and OEC‐M1. β‐actin was used as a loading control. B, Phase contrast images of HNSCC cell lines cultured in 2D, 2.5D, and 3D environments. Scale bar = 10 μm. C‐E, The intracellular stiffness (at frequency f = 10 Hz) of HNSCC cell lines culture in 2D, 2.5D, and 3D environments. The numbers of cells are indicated in each panel. Data represent mean ± SEM ** P

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Epithelial‐mesenchymal transition softens head and neck cancer cells to facilitate migration in 3D environments. Epithelial‐mesenchymal transition softens head and neck cancer cells to facilitate migration in 3D environments

    doi: 10.1111/jcmm.13656

    Figure Lengend Snippet: Extracellular matrix (ECM) architecture influences cell morphology and intracellular stiffness of HNSCC cell lines (FaDu, CAL‐27, SAS and OEC‐M1). A, Western blot of E‐cadherin and vimentin in four head and neck cancer cell lines FaDu, CAL‐27, SAS and OEC‐M1. β‐actin was used as a loading control. B, Phase contrast images of HNSCC cell lines cultured in 2D, 2.5D, and 3D environments. Scale bar = 10 μm. C‐E, The intracellular stiffness (at frequency f = 10 Hz) of HNSCC cell lines culture in 2D, 2.5D, and 3D environments. The numbers of cells are indicated in each panel. Data represent mean ± SEM ** P

    Article Snippet: The antibodies used in this study include the antibodies against E‐cadherin (#4065, Cell Signaling Technology, Inc. Danvers, MA), vimentin (V6630, Sigma‐Aldrich Corp., St. Louis, MO), Snail (#17732, Abcam plc., Cambridge, UK) and Twist1 (#50581, Abcam plc., Cambridge, UK); and β‐actin (MAB1501, Chemicon International Inc., Billerica, MA) was used as a loading control.

    Techniques: Western Blot, Cell Culture

    E-cadherin staining is reduced in the epithelial cells of colon mucosa in UC, and returns to normal level after anti-inflammatory therapy. Immunostaining with anti-E-cadherin antibodies, revealed by a peroxidase/DAB enzymatic reaction. In normal mucosa, all the epithelial cells of the crypts and the surface exhibited an intense staining at their boundaries (scale bars=80 μm ( a ), 20 μm ( c )). In UC mucosa, the staining intensity appeared to be strongly reduced in some zones (scale bars=80 μm ( b ), 20 μm ( d )). Arrows indicate epithelial cells with low or nil E-cadherin staining. After anti-inflammatory therapy E-cadherin immunostaining was superimposable to that of normal colon mucosa (scale bar=80 μm ( e )). Hematoxylin counterstaining.

    Journal: Oncogene

    Article Title: Interleukin 6 downregulates p53 expression and activity by stimulating ribosome biogenesis: a new pathway connecting inflammation to cancer

    doi: 10.1038/onc.2014.1

    Figure Lengend Snippet: E-cadherin staining is reduced in the epithelial cells of colon mucosa in UC, and returns to normal level after anti-inflammatory therapy. Immunostaining with anti-E-cadherin antibodies, revealed by a peroxidase/DAB enzymatic reaction. In normal mucosa, all the epithelial cells of the crypts and the surface exhibited an intense staining at their boundaries (scale bars=80 μm ( a ), 20 μm ( c )). In UC mucosa, the staining intensity appeared to be strongly reduced in some zones (scale bars=80 μm ( b ), 20 μm ( d )). Arrows indicate epithelial cells with low or nil E-cadherin staining. After anti-inflammatory therapy E-cadherin immunostaining was superimposable to that of normal colon mucosa (scale bar=80 μm ( e )). Hematoxylin counterstaining.

    Article Snippet: The sections were subsequently incubated with primary anti-E-cadherin (clone 32A8, Cell Signaling Technology, Beverly, MA, USA), anti-IL-6 (Sigma-Aldrich) and anti-p53 (Novocastra) antibodies diluted in 1% bovine serum albumin in Tris buffer saline overnight at 4 °C using the appropriate dilutions.

    Techniques: Staining, Immunostaining

    X4 tropic virus disrupts cell-cell adhesion and increases pro-inflammatory signaling leading to increased barrier permeability. A. The airway epithelium is formed by aggregates of ciliated cells that are joined together by adhesive proteins like E-cadherin. B. Our data indicates that X4 tropic viruses are able to bind to the CXCR4 receptor on the airway epithelial cells and enter these cells. Binding of X4 virus to the CXCR4 receptor leads to increase in pERK. C. Binding X4 tropic virus to the CXCR4 receptor decreases E-cadherin, increases monolayer permeability, and increases the expression of ICAM-1 contributing to tissue remodeling.

    Journal: PLoS ONE

    Article Title: HIV Impairs Lung Epithelial Integrity and Enters the Epithelium to Promote Chronic Lung Inflammation

    doi: 10.1371/journal.pone.0149679

    Figure Lengend Snippet: X4 tropic virus disrupts cell-cell adhesion and increases pro-inflammatory signaling leading to increased barrier permeability. A. The airway epithelium is formed by aggregates of ciliated cells that are joined together by adhesive proteins like E-cadherin. B. Our data indicates that X4 tropic viruses are able to bind to the CXCR4 receptor on the airway epithelial cells and enter these cells. Binding of X4 virus to the CXCR4 receptor leads to increase in pERK. C. Binding X4 tropic virus to the CXCR4 receptor decreases E-cadherin, increases monolayer permeability, and increases the expression of ICAM-1 contributing to tissue remodeling.

    Article Snippet: Following transfer, blot was blocked in 5% milk then incubated in E-Cadherin primary antibody (Cell Signaling Technology) diluted to a concentration of 1:1000.

    Techniques: Permeability, Binding Assay, Expressing

    HIV reduces the abundance of E-Cadherin. A. Immunofluorescence shows a reduction in E-Cadherin along the basolateral membrane (white arrow) after exposure to X4 tropic virus IIIB at a concentration of p24 (5ng/ml) which was not seen after exposure to R5 tropic virus BaL at the same concentration. B. Western blot probing for E-Cadherin at a dilution of 1:1000 shows marked reduction in abundance of E-Cadherin following four hour basolateral exposure to X4 tropic virus but not R5 tropic virus. (n = 6 BaL and 10 IIIB samples, *p

    Journal: PLoS ONE

    Article Title: HIV Impairs Lung Epithelial Integrity and Enters the Epithelium to Promote Chronic Lung Inflammation

    doi: 10.1371/journal.pone.0149679

    Figure Lengend Snippet: HIV reduces the abundance of E-Cadherin. A. Immunofluorescence shows a reduction in E-Cadherin along the basolateral membrane (white arrow) after exposure to X4 tropic virus IIIB at a concentration of p24 (5ng/ml) which was not seen after exposure to R5 tropic virus BaL at the same concentration. B. Western blot probing for E-Cadherin at a dilution of 1:1000 shows marked reduction in abundance of E-Cadherin following four hour basolateral exposure to X4 tropic virus but not R5 tropic virus. (n = 6 BaL and 10 IIIB samples, *p

    Article Snippet: Following transfer, blot was blocked in 5% milk then incubated in E-Cadherin primary antibody (Cell Signaling Technology) diluted to a concentration of 1:1000.

    Techniques: Immunofluorescence, Concentration Assay, Western Blot

    Patients with HIV have less E-cadherin in their lung epithelium. A. There is a slight decrease in steady state E-cadherin mRNA as detected by RT-qPCR. (n = 5, *p

    Journal: PLoS ONE

    Article Title: HIV Impairs Lung Epithelial Integrity and Enters the Epithelium to Promote Chronic Lung Inflammation

    doi: 10.1371/journal.pone.0149679

    Figure Lengend Snippet: Patients with HIV have less E-cadherin in their lung epithelium. A. There is a slight decrease in steady state E-cadherin mRNA as detected by RT-qPCR. (n = 5, *p

    Article Snippet: Following transfer, blot was blocked in 5% milk then incubated in E-Cadherin primary antibody (Cell Signaling Technology) diluted to a concentration of 1:1000.

    Techniques: Quantitative RT-PCR

    c‐Src inhibitor could not prevent epithelial‐mesenchymal transition ( EMT ) after knockdown of vimentin. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. Then, they were treated with different concentrations of PP 2 for 48 h to detect A, invasion and B, cell migration. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. C, Next, cells were treated with different concentrations of PP 2 for 24 h to measure wound healing. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. Next, cells were treated with different concentrations of PP 2 for 48 h. D, Cell lysates were harvested for immunoblotting with specific antibodies against vimentin, E‐cadherin, N‐cadherin, and Snail. ** P

    Journal: Cancer Science

    Article Title: c‐Src inhibitor selectively inhibits triple‐negative breast cancer overexpressed Vimentin in vitro and in vivo. c‐Src inhibitor selectively inhibits triple‐negative breast cancer overexpressed Vimentin in vitro and in vivo

    doi: 10.1111/cas.13572

    Figure Lengend Snippet: c‐Src inhibitor could not prevent epithelial‐mesenchymal transition ( EMT ) after knockdown of vimentin. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. Then, they were treated with different concentrations of PP 2 for 48 h to detect A, invasion and B, cell migration. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. C, Next, cells were treated with different concentrations of PP 2 for 24 h to measure wound healing. SUM 1315 MO 2 cells and MDA ‐ MB ‐231 cells were transfected with control sh RNA and vimentin sh RNA 1 for 72 h. Next, cells were treated with different concentrations of PP 2 for 48 h. D, Cell lysates were harvested for immunoblotting with specific antibodies against vimentin, E‐cadherin, N‐cadherin, and Snail. ** P

    Article Snippet: Anti‐human Src, p‐Src (Tyr416), Akt, p‐Akt (Thr308), GAPDH, CDK4, cyclin D1, cyclin E1, vimentin, E‐cadherin, and N‐cadherin antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA).

    Techniques: Multiple Displacement Amplification, Transfection, Migration

    APE 1 promotes the metastatic potential of xenograft lung cancer cells in nude mice. One million HCC 827/ IR cells were inoculated subcutaneously into nude mice which were then treated with icotinib at 0 mg/kg, 70 mg/kg alone, and 70 mg/kg together with E3330 at 25 mg/kg (n = 6 mice or 12 xenografts/group). Xenograft growth was measured in two dimensions, and volume was recorded (tumor size (mm 3 ) = (maximum diameter ×minimum diameter 2 )/2) (A). After daily treatment for 12 d, the nude mice were sacrificed, and histologically intact xenografts were collected. The expression of APE 1, E‐cadherin, and vimentin in the xenograft extracts was detected by Western blot (B). EMT analyses of tissue slides were performed via IHC staining of E‐cadherin and vimentin (C). The mean values of the 12 xenografts of each group are shown as the mean ± SD , * Statistically significant difference when compared with the control group ( P

    Journal: Cancer Medicine

    Article Title: The regulatory role of APE1 in epithelial‐to‐mesenchymal transition and in determining EGFR‐TKI responsiveness in non‐small‐cell lung cancer, et al. The regulatory role of APE1 in epithelial‐to‐mesenchymal transition and in determining EGFR‐TKI responsiveness in non‐small‐cell lung cancer

    doi: 10.1002/cam4.1717

    Figure Lengend Snippet: APE 1 promotes the metastatic potential of xenograft lung cancer cells in nude mice. One million HCC 827/ IR cells were inoculated subcutaneously into nude mice which were then treated with icotinib at 0 mg/kg, 70 mg/kg alone, and 70 mg/kg together with E3330 at 25 mg/kg (n = 6 mice or 12 xenografts/group). Xenograft growth was measured in two dimensions, and volume was recorded (tumor size (mm 3 ) = (maximum diameter ×minimum diameter 2 )/2) (A). After daily treatment for 12 d, the nude mice were sacrificed, and histologically intact xenografts were collected. The expression of APE 1, E‐cadherin, and vimentin in the xenograft extracts was detected by Western blot (B). EMT analyses of tissue slides were performed via IHC staining of E‐cadherin and vimentin (C). The mean values of the 12 xenografts of each group are shown as the mean ± SD , * Statistically significant difference when compared with the control group ( P

    Article Snippet: In agreement with APE1 level, vimentin was upregulated in the rebiopsy tissue whereas E‐cadherin was downregulated.

    Techniques: Mouse Assay, Expressing, Western Blot, Immunohistochemistry, Staining

    Manipulations of APE 1 regulate the EMT process. The EMT markers in both cell lines with different APE 1 status were measured by the epithelial marker E‐cadherin or the mesenchymal marker vimentin using Western blot (A), phase contrast microscopy (B), and immunofluorescent staining (C). Following knockdown of APE 1 in HCC 827/ IR and PC ‐9/ ER cells via si RNA transfection, the expression of E‐cadherin, vimentin, and APE 1 was determined by Western blot (D). Following overexpression of APE 1 in EGFR ‐ TKI ‐sensitive cells via lentiviral particles, relative expression levels of E‐cadherin, vimentin, and APE 1 were determined by Western blot (E). Representative images or blots from at least three individual experimental repeats are shown in this figure

    Journal: Cancer Medicine

    Article Title: The regulatory role of APE1 in epithelial‐to‐mesenchymal transition and in determining EGFR‐TKI responsiveness in non‐small‐cell lung cancer, et al. The regulatory role of APE1 in epithelial‐to‐mesenchymal transition and in determining EGFR‐TKI responsiveness in non‐small‐cell lung cancer

    doi: 10.1002/cam4.1717

    Figure Lengend Snippet: Manipulations of APE 1 regulate the EMT process. The EMT markers in both cell lines with different APE 1 status were measured by the epithelial marker E‐cadherin or the mesenchymal marker vimentin using Western blot (A), phase contrast microscopy (B), and immunofluorescent staining (C). Following knockdown of APE 1 in HCC 827/ IR and PC ‐9/ ER cells via si RNA transfection, the expression of E‐cadherin, vimentin, and APE 1 was determined by Western blot (D). Following overexpression of APE 1 in EGFR ‐ TKI ‐sensitive cells via lentiviral particles, relative expression levels of E‐cadherin, vimentin, and APE 1 were determined by Western blot (E). Representative images or blots from at least three individual experimental repeats are shown in this figure

    Article Snippet: In agreement with APE1 level, vimentin was upregulated in the rebiopsy tissue whereas E‐cadherin was downregulated.

    Techniques: Marker, Western Blot, Microscopy, Staining, Transfection, Expressing, Over Expression

    APE 1 is elevated in T790M‐negative EGFR ‐ TKI ‐resistant patients. APE 1 and the EMT biomarkers E‐cadherin and vimentin were assayed by IHC in the tumors of two patients receiving rebiopsy after progression following treatment with first‐generation EGFR ‐ TKI , and these markers were compared with pretreatment biopsy tissue. Both patients underwent rebiopsy at disease progression with core needle, and the tumor was determined to be T790M negative by the Cobas method

    Journal: Cancer Medicine

    Article Title: The regulatory role of APE1 in epithelial‐to‐mesenchymal transition and in determining EGFR‐TKI responsiveness in non‐small‐cell lung cancer, et al. The regulatory role of APE1 in epithelial‐to‐mesenchymal transition and in determining EGFR‐TKI responsiveness in non‐small‐cell lung cancer

    doi: 10.1002/cam4.1717

    Figure Lengend Snippet: APE 1 is elevated in T790M‐negative EGFR ‐ TKI ‐resistant patients. APE 1 and the EMT biomarkers E‐cadherin and vimentin were assayed by IHC in the tumors of two patients receiving rebiopsy after progression following treatment with first‐generation EGFR ‐ TKI , and these markers were compared with pretreatment biopsy tissue. Both patients underwent rebiopsy at disease progression with core needle, and the tumor was determined to be T790M negative by the Cobas method

    Article Snippet: In agreement with APE1 level, vimentin was upregulated in the rebiopsy tissue whereas E‐cadherin was downregulated.

    Techniques: Immunohistochemistry

    Representative example of immunopositivity grade 3 for E-cadherin (original magnification x400).

    Journal: Gastroenterology Research and Practice

    Article Title: The Prognostic Impact of Protein Expression of E-Cadherin-Catenin Complexes Differs between Rectal and Colon Carcinoma

    doi: 10.1155/2010/616023

    Figure Lengend Snippet: Representative example of immunopositivity grade 3 for E-cadherin (original magnification x400).

    Article Snippet: Sections for α -catenin, p120-catenin, and E-cadherin were dried overnight at 50–60°C before pretreatment with Dako PT link (20 minutes in 98°C), and staining in Dako Autostainer (Dako Corporation, Carpinteria, CA), using Dako's EnVision Flex-system, with mouse-linker in p120-catenin and E-cadherin.

    Techniques:

    SNHG20 knockdown regulated several genes related to proliferation and metastasis ( A , B ) The mRNA level of P21, Cyclin D1, Vimentin, and E-cadherin was detected by qRT-PCR in A2780 and CAOV-3 cells transfecting with si-SNHG20-1 or -2 (* P

    Journal: Bioscience Reports

    Article Title: LncRNA SNHG20 predicts a poor prognosis and promotes cell progression in epithelial ovarian cancer

    doi: 10.1042/BSR20182186

    Figure Lengend Snippet: SNHG20 knockdown regulated several genes related to proliferation and metastasis ( A , B ) The mRNA level of P21, Cyclin D1, Vimentin, and E-cadherin was detected by qRT-PCR in A2780 and CAOV-3 cells transfecting with si-SNHG20-1 or -2 (* P

    Article Snippet: As shown in A,B, we found that the mRNAs of P21 and E-cadherin were significantly increased, while Cyclin D1 and Vimentin were down-regulated at post-transfection with SNHG20 siRNA-1 and -2.

    Techniques: Quantitative RT-PCR

    IL-33 decreases the expression of integrin α4β1 and CD62L on JEG3 cells. Following stimulation with 1 ng/ml rhIL-33 for 48 h, the adhesion and invasion-related molecules (integrins, E-cadherin, CD62L and CD44) on JEG3 cells were analyzed by flow cytometry, with the vehicle as the negative control. (A) Quantification of flow cytometry data. (B) Scatter plots of flow cytometry data. The data are expressed as the mean ± standard error. ***P

    Journal: Molecular Medicine Reports

    Article Title: IL-33 restricts invasion and adhesion of trophoblast cell line JEG3 by downregulation of integrin α4β1 and CD62L

    doi: 10.3892/mmr.2017.7085

    Figure Lengend Snippet: IL-33 decreases the expression of integrin α4β1 and CD62L on JEG3 cells. Following stimulation with 1 ng/ml rhIL-33 for 48 h, the adhesion and invasion-related molecules (integrins, E-cadherin, CD62L and CD44) on JEG3 cells were analyzed by flow cytometry, with the vehicle as the negative control. (A) Quantification of flow cytometry data. (B) Scatter plots of flow cytometry data. The data are expressed as the mean ± standard error. ***P

    Article Snippet: Almost 100% of JEG3 cells expressed integrin α3β1, integrin α5β1, integrin α6β1 and E-cadherin ( ).

    Techniques: Expressing, Flow Cytometry, Cytometry, Negative Control

    Effects of NF-κB specific inhibitor BAY-11-7082 on migration and EMT of HLEC-h3 cells. ( A ) Effects of BAY-11-7082 treatment on NF-κB activity in EMT cell model. ( B ) Effects of BAY-11-7082 treatment on cell migration. ( C ) Effects of BAY-11-7082 treatment on E-cadherin expression. ( D ) Effects of BAY-11-7082 treatment on α-SMA expression. Control group was TGF-β-induced transdifferentiated cells. Each experiment was independently repeated 3 times. ** Compared with control group, p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Transforming Growth Factor β1 (TGF-β1)-Stimulated Integrin-Linked Kinase (ILK) Regulates Migration and Epithelial-Mesenchymal Transition (EMT) of Human Lens Epithelial Cells via Nuclear Factor κB (NF-κB)

    doi: 10.12659/MSM.910601

    Figure Lengend Snippet: Effects of NF-κB specific inhibitor BAY-11-7082 on migration and EMT of HLEC-h3 cells. ( A ) Effects of BAY-11-7082 treatment on NF-κB activity in EMT cell model. ( B ) Effects of BAY-11-7082 treatment on cell migration. ( C ) Effects of BAY-11-7082 treatment on E-cadherin expression. ( D ) Effects of BAY-11-7082 treatment on α-SMA expression. Control group was TGF-β-induced transdifferentiated cells. Each experiment was independently repeated 3 times. ** Compared with control group, p

    Article Snippet: In addition, treatment with ILK siRNA and inhibitor significantly inhibited the migration of LECs, increased the expression level of E-cadherin, and decreased the expression level of α-SMA.

    Techniques: Migration, Activity Assay, Expressing

    Effects of TGF-β treatment on migration and EMT of HLEC-h3 cells. ( A ) Effects of TGF-β treatment on ILK expression. ( B ) Effects of TGF-β treatment on cell migration. ( C ) Effects of TGF-β treatment on E-cadherin expression. ( D ) Effects of TGF-β treatment on α-SMA expression. Each experiment was independently repeated 3 times. ** Compared with control group, p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Transforming Growth Factor β1 (TGF-β1)-Stimulated Integrin-Linked Kinase (ILK) Regulates Migration and Epithelial-Mesenchymal Transition (EMT) of Human Lens Epithelial Cells via Nuclear Factor κB (NF-κB)

    doi: 10.12659/MSM.910601

    Figure Lengend Snippet: Effects of TGF-β treatment on migration and EMT of HLEC-h3 cells. ( A ) Effects of TGF-β treatment on ILK expression. ( B ) Effects of TGF-β treatment on cell migration. ( C ) Effects of TGF-β treatment on E-cadherin expression. ( D ) Effects of TGF-β treatment on α-SMA expression. Each experiment was independently repeated 3 times. ** Compared with control group, p

    Article Snippet: In addition, treatment with ILK siRNA and inhibitor significantly inhibited the migration of LECs, increased the expression level of E-cadherin, and decreased the expression level of α-SMA.

    Techniques: Migration, Expressing

    Effects of ILK expression downregulation on NF-κB activity. ( A ) Effects of ILK siRNA treatment on NF-κB activity in EMT cell model. ( B ) Effects of ILK siRNA treatment on cell migration. ( C ) Effects of ILK siRNA treatment on E-cadherin expression. ( D ) Effects of ILK siRNA treatment on α-SMA expression. Each experiment was independently repeated 3 times. Control group was TGF-β-induced transdifferentiated cells. ** Compared with control group, p

    Journal: Medical Science Monitor : International Medical Journal of Experimental and Clinical Research

    Article Title: Transforming Growth Factor β1 (TGF-β1)-Stimulated Integrin-Linked Kinase (ILK) Regulates Migration and Epithelial-Mesenchymal Transition (EMT) of Human Lens Epithelial Cells via Nuclear Factor κB (NF-κB)

    doi: 10.12659/MSM.910601

    Figure Lengend Snippet: Effects of ILK expression downregulation on NF-κB activity. ( A ) Effects of ILK siRNA treatment on NF-κB activity in EMT cell model. ( B ) Effects of ILK siRNA treatment on cell migration. ( C ) Effects of ILK siRNA treatment on E-cadherin expression. ( D ) Effects of ILK siRNA treatment on α-SMA expression. Each experiment was independently repeated 3 times. Control group was TGF-β-induced transdifferentiated cells. ** Compared with control group, p

    Article Snippet: In addition, treatment with ILK siRNA and inhibitor significantly inhibited the migration of LECs, increased the expression level of E-cadherin, and decreased the expression level of α-SMA.

    Techniques: Expressing, Activity Assay, Migration

    N-cadherin determines the site of first neurite growth and subsequently recruits Golgi and centrosome. ( A ) Examples of neurons immunolabelled with anti-βIII-tubulin developing on coverslips coated with alternating stripes of the indicated substrate and PLL. Quantification of neurons with the soma on the interface of the two substrates shows that neurons grow the first neurite preferentially at the N-cadherin facing pole (experimental n =4). Asymmetric contact with either laminin ( n =4) or Tenascin C ( n =3) does not trigger outgrowth of the first neurite (⩾9 neurons/experiment). ( B ) Examples of centrosome position in hippocampal neurons immunolabelled with a neuron-specific antibody (anti-βIII-tubulin) and with an antibody recognizing the centrosome (anti-γ-tubulin) with their soma on the PLL/N-cadherin interface. The centrosome position of neurons was quantified at different developmental stages (round neurons n =5, one neurite n =7, stage 2 neurons n =9, ⩾8 cells/experiment). The centrosome is recruited towards the N-cadherin substrate. ( A , B ) t -Test, * P

    Journal: The EMBO Journal

    Article Title: N-cadherin specifies first asymmetry in developing neurons

    doi: 10.1038/emboj.2012.41

    Figure Lengend Snippet: N-cadherin determines the site of first neurite growth and subsequently recruits Golgi and centrosome. ( A ) Examples of neurons immunolabelled with anti-βIII-tubulin developing on coverslips coated with alternating stripes of the indicated substrate and PLL. Quantification of neurons with the soma on the interface of the two substrates shows that neurons grow the first neurite preferentially at the N-cadherin facing pole (experimental n =4). Asymmetric contact with either laminin ( n =4) or Tenascin C ( n =3) does not trigger outgrowth of the first neurite (⩾9 neurons/experiment). ( B ) Examples of centrosome position in hippocampal neurons immunolabelled with a neuron-specific antibody (anti-βIII-tubulin) and with an antibody recognizing the centrosome (anti-γ-tubulin) with their soma on the PLL/N-cadherin interface. The centrosome position of neurons was quantified at different developmental stages (round neurons n =5, one neurite n =7, stage 2 neurons n =9, ⩾8 cells/experiment). The centrosome is recruited towards the N-cadherin substrate. ( A , B ) t -Test, * P

    Article Snippet: J Neurosci 30 : 10885–10898 [ ] Jossin Y, Cooper JA (2011) Reelin, Rap1 and N-cadherin orient the migration of multipolar neurons in the developing neocortex .

    Techniques:

    N-cadherin distribution in vivo . ( A ) A pair of neurons just generated in the SVZ (note the adjacent Tbr2-positive basal progenitors) show a crescent of N-cadherin GFP towards the CP. ( B ) Paraffin sections from Tubb3–mGFP mice were labelled with N-cadherin. Two examples are shown: the left picture shows the co-labelling of mGFP (new neuron), N-cadherin and DAPI (nucleus). The middle picture shows N-cadherin and DAPI with the outline of the GFP-positive cell drawn and the right picture the intensity of N-cadherin only in this GFP positive selected neuron. Scale bars=5 μm.

    Journal: The EMBO Journal

    Article Title: N-cadherin specifies first asymmetry in developing neurons

    doi: 10.1038/emboj.2012.41

    Figure Lengend Snippet: N-cadherin distribution in vivo . ( A ) A pair of neurons just generated in the SVZ (note the adjacent Tbr2-positive basal progenitors) show a crescent of N-cadherin GFP towards the CP. ( B ) Paraffin sections from Tubb3–mGFP mice were labelled with N-cadherin. Two examples are shown: the left picture shows the co-labelling of mGFP (new neuron), N-cadherin and DAPI (nucleus). The middle picture shows N-cadherin and DAPI with the outline of the GFP-positive cell drawn and the right picture the intensity of N-cadherin only in this GFP positive selected neuron. Scale bars=5 μm.

    Article Snippet: J Neurosci 30 : 10885–10898 [ ] Jossin Y, Cooper JA (2011) Reelin, Rap1 and N-cadherin orient the migration of multipolar neurons in the developing neocortex .

    Techniques: In Vivo, Generated, Mouse Assay

    N-cadherin accumulates in one pole and mediates centrosome repositioning via PI3K and actin. ( A ) Labelling of surface N-cadherin and alignment of maxima of N-cadherin surface fluorescence in round neurons shows the presence of an N-cadherin accumulation (circular graph represents the mean fluorescence at the cell surface respect the maximum: experimental n =3, 15 cells/experiment). ( B ) This N-cadherin accumulation is not always correlated with the Golgi pole as shown by a frequency distribution of the angles between the Golgi and N-cadherin maxima in individual neurons in the circular frequency plot. ( C ) Beads (1 μm diameter) coated with N-cadherin (green) are recruited to the site from which the first neurite is growing (upper panel), while Tenascin C coated control beads (red) are not recruited to any specific site of the cell surface (lower panel). ( D ) N-cadherin is still concentrated at the site from which the first neurite grows, here demonstrated by the polarized surface distribution of N-cadherin and ( E ): the stable attachment of N-cadherin-coated beads. All scale bars=5 μm. ( F , G ) Hippocampal neurons grown in the presence of specific inhibitors on coverslips coated with alternating stripes of N-cadherin and PLL were fixed after different times and immunolabelled with an antibody recognizing the centrosome or the Golgi and the neuron-specific antibody anti-βIII-tubulin. ( F ) Fraction of neurons recruiting the Golgi/centrosome towards N-cadherin. ( G ) Fraction of neurons orienting the first neurite towards N-cadherin. ( H ) Hippocampal neurons were grown on homogeneous PLL in the presence of toxins, fixed and immunolabelled as in ( F , G ). Fraction of neurons grown only on PLL in which the first bud is located at the Golgi/centrosome pole. ( F – H ) ⩾10 neurons/experiment. Experimental n =3. ANOVA followed by Dunnett's test versus control, ** P

    Journal: The EMBO Journal

    Article Title: N-cadherin specifies first asymmetry in developing neurons

    doi: 10.1038/emboj.2012.41

    Figure Lengend Snippet: N-cadherin accumulates in one pole and mediates centrosome repositioning via PI3K and actin. ( A ) Labelling of surface N-cadherin and alignment of maxima of N-cadherin surface fluorescence in round neurons shows the presence of an N-cadherin accumulation (circular graph represents the mean fluorescence at the cell surface respect the maximum: experimental n =3, 15 cells/experiment). ( B ) This N-cadherin accumulation is not always correlated with the Golgi pole as shown by a frequency distribution of the angles between the Golgi and N-cadherin maxima in individual neurons in the circular frequency plot. ( C ) Beads (1 μm diameter) coated with N-cadherin (green) are recruited to the site from which the first neurite is growing (upper panel), while Tenascin C coated control beads (red) are not recruited to any specific site of the cell surface (lower panel). ( D ) N-cadherin is still concentrated at the site from which the first neurite grows, here demonstrated by the polarized surface distribution of N-cadherin and ( E ): the stable attachment of N-cadherin-coated beads. All scale bars=5 μm. ( F , G ) Hippocampal neurons grown in the presence of specific inhibitors on coverslips coated with alternating stripes of N-cadherin and PLL were fixed after different times and immunolabelled with an antibody recognizing the centrosome or the Golgi and the neuron-specific antibody anti-βIII-tubulin. ( F ) Fraction of neurons recruiting the Golgi/centrosome towards N-cadherin. ( G ) Fraction of neurons orienting the first neurite towards N-cadherin. ( H ) Hippocampal neurons were grown on homogeneous PLL in the presence of toxins, fixed and immunolabelled as in ( F , G ). Fraction of neurons grown only on PLL in which the first bud is located at the Golgi/centrosome pole. ( F – H ) ⩾10 neurons/experiment. Experimental n =3. ANOVA followed by Dunnett's test versus control, ** P

    Article Snippet: J Neurosci 30 : 10885–10898 [ ] Jossin Y, Cooper JA (2011) Reelin, Rap1 and N-cadherin orient the migration of multipolar neurons in the developing neocortex .

    Techniques: Fluorescence

    N-cadherin orients the cell axis and influences polarity in vivo . ( A ) Schematic representation of our measurement of cell axis orientation in the developing cortex. Radial direction is perpendicular to the VZ. The angle deviating from radial direction (θ=0) has been quantified in all experiments and displayed as frequency distributions. ( B ) In utero electroporation of the lateral ventricles of E14.5 mice with GFP control vectors, wt N-cadherin–GFP or dominant negative Δ390N-cadherin–GFP. Neurons exiting the SVZ are analysed. ( C ) Representative z -stack projections of neurons analysed in ( B ). The right upper panel shows neurons transfected with Δ390N-cadherin–GFP (E14.5–E16.5), which were pulse labelled for 11 h with BrDU. ( D ) Golgi orientation with respect to the radial direction of the same neurons as in ( B ). ( E ) Quantification of embryonic cortical neurons transfected with wt N-cadherin–GFP, dominant negative Δ390 N-cadherin–GFP or dt-Tomato (internal control) were grown on coronal cortical sections. ( B , D , E ) All insets: population distribution ( *** P

    Journal: The EMBO Journal

    Article Title: N-cadherin specifies first asymmetry in developing neurons

    doi: 10.1038/emboj.2012.41

    Figure Lengend Snippet: N-cadherin orients the cell axis and influences polarity in vivo . ( A ) Schematic representation of our measurement of cell axis orientation in the developing cortex. Radial direction is perpendicular to the VZ. The angle deviating from radial direction (θ=0) has been quantified in all experiments and displayed as frequency distributions. ( B ) In utero electroporation of the lateral ventricles of E14.5 mice with GFP control vectors, wt N-cadherin–GFP or dominant negative Δ390N-cadherin–GFP. Neurons exiting the SVZ are analysed. ( C ) Representative z -stack projections of neurons analysed in ( B ). The right upper panel shows neurons transfected with Δ390N-cadherin–GFP (E14.5–E16.5), which were pulse labelled for 11 h with BrDU. ( D ) Golgi orientation with respect to the radial direction of the same neurons as in ( B ). ( E ) Quantification of embryonic cortical neurons transfected with wt N-cadherin–GFP, dominant negative Δ390 N-cadherin–GFP or dt-Tomato (internal control) were grown on coronal cortical sections. ( B , D , E ) All insets: population distribution ( *** P

    Article Snippet: J Neurosci 30 : 10885–10898 [ ] Jossin Y, Cooper JA (2011) Reelin, Rap1 and N-cadherin orient the migration of multipolar neurons in the developing neocortex .

    Techniques: In Vivo, In Utero, Electroporation, Mouse Assay, Dominant Negative Mutation, Transfection

    Immunofluorescent profile of BST2 and E-cadherin in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (20,000/well) were seeded on the coverslips in 24-well cell culture plates and cultured overnight at 37°C in 5% CO 2 and 70% relative humidity. The next day, cells were treated with and without IFN-γ for 24 h followed by immunolocalization of BST2, E-cadherin, and actin as described in Materials and Methods . Panel a shows the expression of actin (red) and BST2 (green) in control and IFN-γ-treated HTR-8/SVneo cells. Panel b shows the expression of actin (red) and E-cadherin (green) in control and IFN-γ-treated cells. The nuclei were stained using Hoechst nuclear staining dye. Scale bar shows 20 μm.

    Journal: Cell Adhesion & Migration

    Article Title: BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin

    doi: 10.1080/19336918.2019.1710024

    Figure Lengend Snippet: Immunofluorescent profile of BST2 and E-cadherin in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (20,000/well) were seeded on the coverslips in 24-well cell culture plates and cultured overnight at 37°C in 5% CO 2 and 70% relative humidity. The next day, cells were treated with and without IFN-γ for 24 h followed by immunolocalization of BST2, E-cadherin, and actin as described in Materials and Methods . Panel a shows the expression of actin (red) and BST2 (green) in control and IFN-γ-treated HTR-8/SVneo cells. Panel b shows the expression of actin (red) and E-cadherin (green) in control and IFN-γ-treated cells. The nuclei were stained using Hoechst nuclear staining dye. Scale bar shows 20 μm.

    Article Snippet: Further, cells were incubated with 1:50 dilution of primary antibody against either BST2 (Santa Cruz Biotechnology Inc., # sc-390719) or E-cadherin (Cloud-Clone Corp., #MAA017Hu22) in 3% BSA in PBS for 2 h at RT.

    Techniques: Cell Culture, Expressing, Staining

    Role of BST2, E-cadherin, and signaling pathways in IFN-γ-mediated decrease in invasion of HTR-8/SVneo cells. Treatment of HTR-8/SVneo cells with IFN-γ leads to upregulation in the expression of BST2 and concomitantly activates JAK/STAT and AKT signaling pathways. Silencing or inhibition of BST2 and AKT signaling pathway abrogates the effect of IFN-γ on the decreased invasion of HTR-8/SVneo cells. Further, silencing of STAT1 and inhibition of AKT signaling pathways also inhibit the expression of BST2. Interestingly, the expression of E-cadherin was regulated by STAT1 and AKT signaling pathways and also by levels of BST2, which suggest their role in IFN-γ-mediated decrease in invasion of HTR-8/SVneo cells.

    Journal: Cell Adhesion & Migration

    Article Title: BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin

    doi: 10.1080/19336918.2019.1710024

    Figure Lengend Snippet: Role of BST2, E-cadherin, and signaling pathways in IFN-γ-mediated decrease in invasion of HTR-8/SVneo cells. Treatment of HTR-8/SVneo cells with IFN-γ leads to upregulation in the expression of BST2 and concomitantly activates JAK/STAT and AKT signaling pathways. Silencing or inhibition of BST2 and AKT signaling pathway abrogates the effect of IFN-γ on the decreased invasion of HTR-8/SVneo cells. Further, silencing of STAT1 and inhibition of AKT signaling pathways also inhibit the expression of BST2. Interestingly, the expression of E-cadherin was regulated by STAT1 and AKT signaling pathways and also by levels of BST2, which suggest their role in IFN-γ-mediated decrease in invasion of HTR-8/SVneo cells.

    Article Snippet: Further, cells were incubated with 1:50 dilution of primary antibody against either BST2 (Santa Cruz Biotechnology Inc., # sc-390719) or E-cadherin (Cloud-Clone Corp., #MAA017Hu22) in 3% BSA in PBS for 2 h at RT.

    Techniques: Expressing, Inhibition

    Expression of BST2 and E-cadherin after AKT inhibition in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10 6 /well) were cultured overnight in 6-well cell culture plate at 37°C in 5% CO 2 and 70% relative humidity. The next day, cells were pretreated in the presence and absence of LY294002 (50 μM) for 2 h followed by treatment with IFN-γ (10 ng/mL) for 24 h and used to study the expression of BST2 and E-cadherin by Western blotting as described in Materials and Methods . Panel a shows the bar graph of BST2 at the protein level in untreated and pretreated LY294002 cells, respectively, on treatment with and without IFN-γ. Panel b shows the expression of E-cadherin at the protein level in untreated and LY294002 pretreated cells, respectively, on subsequent treatment with and without IFN-γ. Each bar represents relative expression after normalization with GAPDH with respect to LY294002 untreated control cells and without treatment of IFN-γ. Values are expressed as mean ± SEM of three independent experiments. Representative blots of BST2, E-cadherin, and GAPDH from one of the three independent experiments are appended with respective panels.

    Journal: Cell Adhesion & Migration

    Article Title: BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin

    doi: 10.1080/19336918.2019.1710024

    Figure Lengend Snippet: Expression of BST2 and E-cadherin after AKT inhibition in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10 6 /well) were cultured overnight in 6-well cell culture plate at 37°C in 5% CO 2 and 70% relative humidity. The next day, cells were pretreated in the presence and absence of LY294002 (50 μM) for 2 h followed by treatment with IFN-γ (10 ng/mL) for 24 h and used to study the expression of BST2 and E-cadherin by Western blotting as described in Materials and Methods . Panel a shows the bar graph of BST2 at the protein level in untreated and pretreated LY294002 cells, respectively, on treatment with and without IFN-γ. Panel b shows the expression of E-cadherin at the protein level in untreated and LY294002 pretreated cells, respectively, on subsequent treatment with and without IFN-γ. Each bar represents relative expression after normalization with GAPDH with respect to LY294002 untreated control cells and without treatment of IFN-γ. Values are expressed as mean ± SEM of three independent experiments. Representative blots of BST2, E-cadherin, and GAPDH from one of the three independent experiments are appended with respective panels.

    Article Snippet: Further, cells were incubated with 1:50 dilution of primary antibody against either BST2 (Santa Cruz Biotechnology Inc., # sc-390719) or E-cadherin (Cloud-Clone Corp., #MAA017Hu22) in 3% BSA in PBS for 2 h at RT.

    Techniques: Expressing, Inhibition, Cell Culture, Western Blot

    Expression of E-cadherin after STAT1 silencing in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10 6 /well) were grown overnight in 6-well culture plate at 37°C in 5% CO 2 and 70% relative humidity. Subsequently, cells were transfected with STAT1 and scrambled siRNA and used to study the protein level of E-cadherin by Western blotting as described in Materials and Methods . The bar graph shows the expression of E-cadherin at the protein level in scrambled siRNA and STAT1 siRNA-transfected cells, respectively, on treatment with and without IFN-γ (10 ng/mL). Representative blots of E-cadherin and GAPDH from one of the three independent experiments are appended. Each bar represents the relative expression of E-cadherin after normalization with GAPDH with respect to untreated scrambled siRNA-transfected cells. Values are expressed as mean ± SEM of three independent experiments.

    Journal: Cell Adhesion & Migration

    Article Title: BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin

    doi: 10.1080/19336918.2019.1710024

    Figure Lengend Snippet: Expression of E-cadherin after STAT1 silencing in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10 6 /well) were grown overnight in 6-well culture plate at 37°C in 5% CO 2 and 70% relative humidity. Subsequently, cells were transfected with STAT1 and scrambled siRNA and used to study the protein level of E-cadherin by Western blotting as described in Materials and Methods . The bar graph shows the expression of E-cadherin at the protein level in scrambled siRNA and STAT1 siRNA-transfected cells, respectively, on treatment with and without IFN-γ (10 ng/mL). Representative blots of E-cadherin and GAPDH from one of the three independent experiments are appended. Each bar represents the relative expression of E-cadherin after normalization with GAPDH with respect to untreated scrambled siRNA-transfected cells. Values are expressed as mean ± SEM of three independent experiments.

    Article Snippet: Further, cells were incubated with 1:50 dilution of primary antibody against either BST2 (Santa Cruz Biotechnology Inc., # sc-390719) or E-cadherin (Cloud-Clone Corp., #MAA017Hu22) in 3% BSA in PBS for 2 h at RT.

    Techniques: Expressing, Transfection, Western Blot

    Expression of E-cadherin after BST2 silencing in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10 6 /well) were cultured overnight in 6-well culture plate at 37°C in 5% CO 2 and 70% relative humidity and subsequently transfected with BST2 and scrambled siRNA. The scrambled siRNA- and BST2 siRNA-transfected cells were used to study the expression of E-cadherin by Western blotting using a mouse monoclonal antibody against E-cadherin as described in Materials and Methods . The bar graph shows the expression of E-cadherin at protein level in scrambled siRNA-transfected and BST2-silenced cells, respectively, on treatment with and without IFN-γ (10 ng/mL) and the representative blots of E-cadherin and GAPDH from one of the three experiments are appended. Each bar represents relative expression after normalization with GAPDH with respect to untreated scrambled siRNA-transfected cells. Values are expressed as mean ± SEM of three independent experiments.

    Journal: Cell Adhesion & Migration

    Article Title: BST2 regulates interferon gamma-dependent decrease in invasion of HTR-8/SVneo cells via STAT1 and AKT signaling pathways and expression of E-cadherin

    doi: 10.1080/19336918.2019.1710024

    Figure Lengend Snippet: Expression of E-cadherin after BST2 silencing in HTR-8/SVneo cells treated with IFN-γ. HTR-8/SVneo cells (0.1 × 10 6 /well) were cultured overnight in 6-well culture plate at 37°C in 5% CO 2 and 70% relative humidity and subsequently transfected with BST2 and scrambled siRNA. The scrambled siRNA- and BST2 siRNA-transfected cells were used to study the expression of E-cadherin by Western blotting using a mouse monoclonal antibody against E-cadherin as described in Materials and Methods . The bar graph shows the expression of E-cadherin at protein level in scrambled siRNA-transfected and BST2-silenced cells, respectively, on treatment with and without IFN-γ (10 ng/mL) and the representative blots of E-cadherin and GAPDH from one of the three experiments are appended. Each bar represents relative expression after normalization with GAPDH with respect to untreated scrambled siRNA-transfected cells. Values are expressed as mean ± SEM of three independent experiments.

    Article Snippet: Further, cells were incubated with 1:50 dilution of primary antibody against either BST2 (Santa Cruz Biotechnology Inc., # sc-390719) or E-cadherin (Cloud-Clone Corp., #MAA017Hu22) in 3% BSA in PBS for 2 h at RT.

    Techniques: Expressing, Cell Culture, Transfection, Western Blot

    Defining characteristics of (A-F) epithelial and (G-L) epithelial-mesenchymal transition (EMT) tumors. (A) Large blood vessels and solid nests of tumor cells separated by connective tissue are observed in the microscopic H E sections. (G) The EMT tumor architecture is characterized by a heterogeneous population of cells, including loosely packed, elongated (spindle) tumor cells (B-F, H-L, M). During EMT there is a significant reduction in E-cadherin, CK8/18 and CK19 expression, with a concomitant upregulation of vimentin. (N) Without ultrasound, the EMT phenotype is also associated with lower liposomal accumulation than epithelial tumors, although the rate of accumulation was higher for EMT. (*P

    Journal: Cancer Research

    Article Title: Ultrasound increases nanoparticle delivery by reducing intratumoral pressure and increasing transport in epithelial and epithelial-mesenchymal transition tumors

    doi: 10.1158/0008-5472.CAN-11-3232

    Figure Lengend Snippet: Defining characteristics of (A-F) epithelial and (G-L) epithelial-mesenchymal transition (EMT) tumors. (A) Large blood vessels and solid nests of tumor cells separated by connective tissue are observed in the microscopic H E sections. (G) The EMT tumor architecture is characterized by a heterogeneous population of cells, including loosely packed, elongated (spindle) tumor cells (B-F, H-L, M). During EMT there is a significant reduction in E-cadherin, CK8/18 and CK19 expression, with a concomitant upregulation of vimentin. (N) Without ultrasound, the EMT phenotype is also associated with lower liposomal accumulation than epithelial tumors, although the rate of accumulation was higher for EMT. (*P

    Article Snippet: N-cadherin was highly expressed in both tumor phenotypes (0.68±0.02 and 0.64±0.02), but was bound to the plasma membrane in epithelial tumors versus cytoplasmic in EMT tumors.

    Techniques: Expressing

    Slit2/Robo1 signaling activates the Src-mediated inhibition of E-cadherin IHC analysis of the expression of total Src and pSrc (Tyr416) in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (A). Inactivation of Slit2/Robo1 significantly reduced the expression of pSrc (Tyr 416) in SW620 and SW480 cells, and activation of Slit2/Robo1 signaling through overexpressing Slit2 or Robo1 expression promotes pSrc (Tyr 416) expression in HCT-116 cells (B). IHC analysis of the expression of E-cadherin in the tumor tissues of the Apc Min/+ and Apc Min/+ ; Slit2 mice (C). Inactivation of Src signaling significantly enhances the expression of E-cadherin but inhibits the expression of β-cateninin in SW620 and SW480 cells (D). Src inactivation in SW620 and SW480 cells also led to a reduced nuclear translocation of β-catenin (E). Inhibition of E-cadherin expression through siRNA technique could promote the expression of β-cateninin and vimentin in SW620 and SW480 cells (F). IHC analysis of the expression of vimentin in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (G). The data in IHC staining are representative of 11 mice per group (All mice were 24-week-old). The results of IHC were determined using IPP software, and the data were expressed as the mean ±S.D. *: P

    Journal: Oncotarget

    Article Title: Slit2/Robo1 signaling promotes intestinal tumorigenesis through Src-mediated activation of the Wnt/β-catenin pathway

    doi:

    Figure Lengend Snippet: Slit2/Robo1 signaling activates the Src-mediated inhibition of E-cadherin IHC analysis of the expression of total Src and pSrc (Tyr416) in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (A). Inactivation of Slit2/Robo1 significantly reduced the expression of pSrc (Tyr 416) in SW620 and SW480 cells, and activation of Slit2/Robo1 signaling through overexpressing Slit2 or Robo1 expression promotes pSrc (Tyr 416) expression in HCT-116 cells (B). IHC analysis of the expression of E-cadherin in the tumor tissues of the Apc Min/+ and Apc Min/+ ; Slit2 mice (C). Inactivation of Src signaling significantly enhances the expression of E-cadherin but inhibits the expression of β-cateninin in SW620 and SW480 cells (D). Src inactivation in SW620 and SW480 cells also led to a reduced nuclear translocation of β-catenin (E). Inhibition of E-cadherin expression through siRNA technique could promote the expression of β-cateninin and vimentin in SW620 and SW480 cells (F). IHC analysis of the expression of vimentin in the tumor tissues of Apc Min/+ and Apc Min/+ ; Slit2 mice (G). The data in IHC staining are representative of 11 mice per group (All mice were 24-week-old). The results of IHC were determined using IPP software, and the data were expressed as the mean ±S.D. *: P

    Article Snippet: The siRNA duplex for Slit2 was obtained from Santa Cruz Biotechnology Inc. (Santa Cruz, California, USA), and the siRNA duplexes for Robo1, Src and E-cadherin were obtained from Guangzhou RiboBio Co., Ltd. (Guangzhou, China).

    Techniques: Inhibition, Immunohistochemistry, Expressing, Mouse Assay, Activation Assay, Translocation Assay, Staining, Software

    Pooled transcript expression corrected to β actin micro-dissected archival FFPE diagnostic biopsies of men treated by EBRT or PADT and stratified by early biochemical relapse or no-relapse. A . Expression of MSMB B . Expression of E Cadherin (*p = 0.02, **p

    Journal: BMC Cancer

    Article Title: Multi-transcript profiling in archival diagnostic prostate cancer needle biopsies to evaluate biomarkers in non-surgically treated men

    doi: 10.1186/1471-2407-14-673

    Figure Lengend Snippet: Pooled transcript expression corrected to β actin micro-dissected archival FFPE diagnostic biopsies of men treated by EBRT or PADT and stratified by early biochemical relapse or no-relapse. A . Expression of MSMB B . Expression of E Cadherin (*p = 0.02, **p

    Article Snippet: Immunoreactivity signals for E Cadherin were assessed as being absent or weak (0/+) and moderate or strong (++/+++).

    Techniques: Expressing, Formalin-fixed Paraffin-Embedded, Diagnostic Assay

    Immunohistochemistry in diagnostic biopsies from EBRT treated men. A . Low Ki67 expression B . High Ki67 expression. C . Low E Cadherin expression. D . High E Cadherin expression (X40 magnification for all images).

    Journal: BMC Cancer

    Article Title: Multi-transcript profiling in archival diagnostic prostate cancer needle biopsies to evaluate biomarkers in non-surgically treated men

    doi: 10.1186/1471-2407-14-673

    Figure Lengend Snippet: Immunohistochemistry in diagnostic biopsies from EBRT treated men. A . Low Ki67 expression B . High Ki67 expression. C . Low E Cadherin expression. D . High E Cadherin expression (X40 magnification for all images).

    Article Snippet: Immunoreactivity signals for E Cadherin were assessed as being absent or weak (0/+) and moderate or strong (++/+++).

    Techniques: Immunohistochemistry, Diagnostic Assay, Expressing

    E-cadherin is only expressed in ameloblasts capable of expressing p120. In the same K14-Cre p120-cKO mosaic incisor shown in Figure 8 , E-cadherin (top) and p120 catenin (bottom) was immunolocalized in adjacent cross-sections. E-cadherin is expressed exclusively in normal appearing ameloblasts (brackets) that also express p120. However, in flattened, malformed ameloblasts where p120 was ablated, immunostaining for E-cadherin was not detectable. EO, enamel organ; PO, pulp organ.

    Journal: PLoS ONE

    Article Title: Targeted p120-Catenin Ablation Disrupts Dental Enamel Development

    doi: 10.1371/journal.pone.0012703

    Figure Lengend Snippet: E-cadherin is only expressed in ameloblasts capable of expressing p120. In the same K14-Cre p120-cKO mosaic incisor shown in Figure 8 , E-cadherin (top) and p120 catenin (bottom) was immunolocalized in adjacent cross-sections. E-cadherin is expressed exclusively in normal appearing ameloblasts (brackets) that also express p120. However, in flattened, malformed ameloblasts where p120 was ablated, immunostaining for E-cadherin was not detectable. EO, enamel organ; PO, pulp organ.

    Article Snippet: E-cadherin and N-cadherin were designed as listed in the Roche Universal Probe Library Assay Design Center ( http://qpcr.probefinder.com/organism.jsp ).

    Techniques: Expressing, Immunostaining

    N-cadherin is expressed in wild-type secretory stage ameloblasts, but not in p120 ablated ameloblasts. In the less mature second molar (M2), N-cadherin was not expressed (A) in the enamel organ (EO) or pulp organ (PO) of three day-old mice. In the more mature first molar (M1), N-cadherin was expressed (B, C). After dentin matrix deposition, odontoblasts (Od) and ameloblasts (Am) showed lateral membrane immunostaining for N-cadherin, and the apical and basal terminal web apparatus of ameloblasts were also stained positively (B). After enamel matrix deposition, the odontoblasts stain intensely (C). A developing cusp tip from the first molar of a P3 K14-Cre p120-cKO mouse stained for N-cadherin (D). N-cadherin expression was detected in odontoblasts, but not in the ameloblasts from this molar. Note that the dentin appears rough and mildly dysplastic.

    Journal: PLoS ONE

    Article Title: Targeted p120-Catenin Ablation Disrupts Dental Enamel Development

    doi: 10.1371/journal.pone.0012703

    Figure Lengend Snippet: N-cadherin is expressed in wild-type secretory stage ameloblasts, but not in p120 ablated ameloblasts. In the less mature second molar (M2), N-cadherin was not expressed (A) in the enamel organ (EO) or pulp organ (PO) of three day-old mice. In the more mature first molar (M1), N-cadherin was expressed (B, C). After dentin matrix deposition, odontoblasts (Od) and ameloblasts (Am) showed lateral membrane immunostaining for N-cadherin, and the apical and basal terminal web apparatus of ameloblasts were also stained positively (B). After enamel matrix deposition, the odontoblasts stain intensely (C). A developing cusp tip from the first molar of a P3 K14-Cre p120-cKO mouse stained for N-cadherin (D). N-cadherin expression was detected in odontoblasts, but not in the ameloblasts from this molar. Note that the dentin appears rough and mildly dysplastic.

    Article Snippet: E-cadherin and N-cadherin were designed as listed in the Roche Universal Probe Library Assay Design Center ( http://qpcr.probefinder.com/organism.jsp ).

    Techniques: Mouse Assay, Immunostaining, Staining, Expressing