Journal: PLoS ONE
Article Title: Self DNA from Lymphocytes That Have Undergone Activation-Induced Cell Death Enhances Murine B Cell Proliferation and Antibody Production
Figure Lengend Snippet: Promotion of plasma cell differentiation and Ig production in lupus B cells by ALD-DNA. Splenic B cells from 16- to 20-week-old MRL/lpr mice and 12-week-old MRL +/+ mice were cultured in media alone, or in the presence of ALD-DNA (50 µg/ml), LPS (100 ng/ml), or both for different periods as indicated below. ( A ) After 72 h stimulation, viable cells were analyzed for B220 and CD138 surface expression by flow cytometry. Representative dot plots showed the frequencies of plasmablasts (B220 + CD138 hi , lower panels, MRL/lpr, n = 6) or plasma cells (B220 − CD138 + , upper panels, MRL +/+ , n = 6) generated after each treatment. ( B ) Expression of Blimp-1 and XBP1 mRNA from lupus B cells cultured for 96 h was examined by real-time PCR. Results are expressed as the fold induction of gene transcription relative to their untreated controls (means ±SEM, n = 6). ( C ) Ig production in cell culture supernatants harvested on day 6 was determined by ELISA. Bars represent the means ±SEM of three independent experiments (n = 6). * P
Article Snippet: CFSE-labeled B cells stimulated with ALD-DNA, E. coli DNA, LPS, anti-CD40, or different combinations of these stimuli for 3 days were stained with B220-APC (RA3-6B2, eBioscience) or CD138-APC (281-2, BD Biosciences).
Techniques: Cell Differentiation, Mouse Assay, Cell Culture, Expressing, Flow Cytometry, Cytometry, Generated, Real-time Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay