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  • 85
    ATCC e coli dh5α
    Southern blot analysis of traC in E. coli and P. stewartii subsp. stewartii . (A) Five micrograms of DNA from E. coli <t>DH5α</t> (lane 1) and E. coli HB101 (lane 2) was digested with BglII/NotI. Lane 3 was loaded with P. stewartii subsp. stewartii SW2
    E Coli Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 331 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Millipore e coli dh5α
    Comparison of bioluminescence from S. aureus and E. coli containing the native luxCDABE operon versus that in strains containing the modified luxABCDE operon. Exponential cultures of S. aureus RN4220 pMK4 luxABCDE P1 (-■-), S. aureus RN4220 pMK4 luxCDABE P1 (-▴-), E. coli <t>DH5α</t> pMK4 luxABCDE P1 (‥■‥) and E. coli DH5α pMK4 luxCDABE P1 (‥▴‥) were diluted across black 96-well microtiter plates in doubling dilutions (−0.3 log) and monitored for light over a period of 30 min using the ICCD camera. The content of each well was then plated to allow the number of CFU to be compared to the level of bioluminescence (RLU).
    E Coli Dh5α, supplied by Millipore, used in various techniques. Bioz Stars score: 99/100, based on 742 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli e coli dh5α
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    Escherichia Coli E Coli Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 91/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli dh5α f
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    E Coli Dh5α F, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli dh5α noninvasive
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    E Coli Dh5α Noninvasive, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli strain dh5α
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    E Coli Strain Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC laboratory e coli dh5α
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    Laboratory E Coli Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa e coli anaerobic broth escherichia coli dh5α
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    E Coli Anaerobic Broth Escherichia Coli Dh5α, supplied by TaKaRa, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC bacteria e coli dh5α
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    Bacteria E Coli Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    ATCC e coli k12 dh5α
    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli <t>DH5α;</t> panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.
    E Coli K12 Dh5α, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 9 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Stratagene e coli dh5α
    Strategy for gene replacement in Streptomyces . ( A )IV (Apra R ), oriT of RK2, and two FRT sites was amplified by PCR by using primers containing 39-nt 5′ homology extensions corresponding to regions flanking the S. coelicolor target gene (dotted lines a and b) and 20-nt 3′ homology to the unique priming sites P1 and P2 of the disruption cassette. ( B ) The PCR product from A was used to transform E. coli BW25113/pIJ790 (expressing the λ-Red recombination functions), containing the Supercos-1 cosmid with the cloned target gene ( cyc2 ). ( C ) Apra R transformants were selected, and the recombinant cosmid was identified by PCR and restriction analysis. ( D ) The potent methyl-specific restriction system of S. coelicolor was circumvented by introducing the recombinant cosmid into the methylation-deficient E. coli host ET12567/pUZ8002. The cosmid was mobilized in trans by pUZ8002 to Streptomyces by conjugation. ( E ) Selected exconjugants (Apra R ) were screened for Kan S (loss of the Kan resistance gene neo ), and the double cross-over allelic exchange was confirmed by PCR and Southern blot analysis. ( F ) E. coli <t>DH5α</t> cells containing pCP20 were transformed with the mutagenized cosmid DNA, and FLP synthesis was induced. ( G) The loss of the disruption resistance marker was tested, and the cosmid was introduced by polyethylene glycol (PEG)-mediated transformation into the S. coelicolor mutant carrying a marked deletion within the gene of interest. ( H ) Kan R transformants arising from single cross-over events were restreaked nonselectively and screened for the loss of both Kan R and Apra R , indicating a successful replacement by the in-frame deletion marked only by a “scar” sequence of 81 bp containing priming sites P1 and P2 (underlined), and one FRT site.
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    TransGen biotech co escherichia coli dh5α
    Strategy for gene replacement in Streptomyces . ( A )IV (Apra R ), oriT of RK2, and two FRT sites was amplified by PCR by using primers containing 39-nt 5′ homology extensions corresponding to regions flanking the S. coelicolor target gene (dotted lines a and b) and 20-nt 3′ homology to the unique priming sites P1 and P2 of the disruption cassette. ( B ) The PCR product from A was used to transform E. coli BW25113/pIJ790 (expressing the λ-Red recombination functions), containing the Supercos-1 cosmid with the cloned target gene ( cyc2 ). ( C ) Apra R transformants were selected, and the recombinant cosmid was identified by PCR and restriction analysis. ( D ) The potent methyl-specific restriction system of S. coelicolor was circumvented by introducing the recombinant cosmid into the methylation-deficient E. coli host ET12567/pUZ8002. The cosmid was mobilized in trans by pUZ8002 to Streptomyces by conjugation. ( E ) Selected exconjugants (Apra R ) were screened for Kan S (loss of the Kan resistance gene neo ), and the double cross-over allelic exchange was confirmed by PCR and Southern blot analysis. ( F ) E. coli <t>DH5α</t> cells containing pCP20 were transformed with the mutagenized cosmid DNA, and FLP synthesis was induced. ( G) The loss of the disruption resistance marker was tested, and the cosmid was introduced by polyethylene glycol (PEG)-mediated transformation into the S. coelicolor mutant carrying a marked deletion within the gene of interest. ( H ) Kan R transformants arising from single cross-over events were restreaked nonselectively and screened for the loss of both Kan R and Apra R , indicating a successful replacement by the in-frame deletion marked only by a “scar” sequence of 81 bp containing priming sites P1 and P2 (underlined), and one FRT site.
    Escherichia Coli Dh5α, supplied by TransGen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 155 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    TaKaRa e coli dh5α
    Western blot analysis of urease extracts by using anti-UreB. Cytosolic protein extracts (10 μg) were electrophoresed through an SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with anti-UreB as described in the Materials and Methods section. (A) Extracts from <t>DH5α</t> (pHP8080/pBluescript) and DH5α (pHP8080/pUEFs). (B) Extracts from SE5000 (pHP8080/pBluescript) and SE5000 (pHP8080/pUDFs). Density values are shown below individual blots. Results are representative of three experiments performed by using extracts prepared on 3 separate days. pBS, pBluescript; 808, pHP808; 8080, pHP8080.
    E Coli Dh5α, supplied by TaKaRa, used in various techniques. Bioz Stars score: 92/100, based on 760 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Promega escherichia coli e coli strain dh5α
    Western blot analysis of urease extracts by using anti-UreB. Cytosolic protein extracts (10 μg) were electrophoresed through an SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with anti-UreB as described in the Materials and Methods section. (A) Extracts from <t>DH5α</t> (pHP8080/pBluescript) and DH5α (pHP8080/pUEFs). (B) Extracts from SE5000 (pHP8080/pBluescript) and SE5000 (pHP8080/pUDFs). Density values are shown below individual blots. Results are representative of three experiments performed by using extracts prepared on 3 separate days. pBS, pBluescript; 808, pHP808; 8080, pHP8080.
    Escherichia Coli E Coli Strain Dh5α, supplied by Promega, used in various techniques. Bioz Stars score: 88/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher escherichia coli strains e coli strain dh5α
    Western blot analysis of urease extracts by using anti-UreB. Cytosolic protein extracts (10 μg) were electrophoresed through an SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with anti-UreB as described in the Materials and Methods section. (A) Extracts from <t>DH5α</t> (pHP8080/pBluescript) and DH5α (pHP8080/pUEFs). (B) Extracts from SE5000 (pHP8080/pBluescript) and SE5000 (pHP8080/pUDFs). Density values are shown below individual blots. Results are representative of three experiments performed by using extracts prepared on 3 separate days. pBS, pBluescript; 808, pHP808; 8080, pHP8080.
    Escherichia Coli Strains E Coli Strain Dh5α, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    tiangen biotech co e coli dh5α
    a , b , c Response surface 3D model (left) and contour plot (right) to assessthe effects of the three variables on the yield of plasmid DNA vaccine pcDNA-CCOL2A1 produced by the engineered E. coli <t>DH5α.</t> a response surface plot showing the mutual effect of peptone and mannitol on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; b response surface plot showing the mutual effect of peptone and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; c response surface plot showing the mutual effect of mannitol and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1
    E Coli Dh5α, supplied by tiangen biotech co, used in various techniques. Bioz Stars score: 93/100, based on 223 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Promega e coli dh5α
    a , b , c Response surface 3D model (left) and contour plot (right) to assessthe effects of the three variables on the yield of plasmid DNA vaccine pcDNA-CCOL2A1 produced by the engineered E. coli <t>DH5α.</t> a response surface plot showing the mutual effect of peptone and mannitol on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; b response surface plot showing the mutual effect of peptone and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; c response surface plot showing the mutual effect of mannitol and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1
    E Coli Dh5α, supplied by Promega, used in various techniques. Bioz Stars score: 93/100, based on 351 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli dh5α/product/Promega
    Average 93 stars, based on 351 article reviews
    Price from $9.99 to $1999.99
    e coli dh5α - by Bioz Stars, 2020-08
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    Image Search Results


    Southern blot analysis of traC in E. coli and P. stewartii subsp. stewartii . (A) Five micrograms of DNA from E. coli DH5α (lane 1) and E. coli HB101 (lane 2) was digested with BglII/NotI. Lane 3 was loaded with P. stewartii subsp. stewartii SW2

    Journal: Journal of Bacteriology

    Article Title: Stabilization of pSW100 from Pantoea stewartii by the F Conjugation System

    doi: 10.1128/JB.00846-07

    Figure Lengend Snippet: Southern blot analysis of traC in E. coli and P. stewartii subsp. stewartii . (A) Five micrograms of DNA from E. coli DH5α (lane 1) and E. coli HB101 (lane 2) was digested with BglII/NotI. Lane 3 was loaded with P. stewartii subsp. stewartii SW2

    Article Snippet: However, pSW140K was extremely unstable in E. coli DH5α, E. coli ATCC 23744, and E. coli ATCC 23846; 95%, 94%, and 77% of the population, respectively, had lost the plasmid after 84 generations of culturing (Fig. ).

    Techniques: Southern Blot

    Comparison of bioluminescence from S. aureus and E. coli containing the native luxCDABE operon versus that in strains containing the modified luxABCDE operon. Exponential cultures of S. aureus RN4220 pMK4 luxABCDE P1 (-■-), S. aureus RN4220 pMK4 luxCDABE P1 (-▴-), E. coli DH5α pMK4 luxABCDE P1 (‥■‥) and E. coli DH5α pMK4 luxCDABE P1 (‥▴‥) were diluted across black 96-well microtiter plates in doubling dilutions (−0.3 log) and monitored for light over a period of 30 min using the ICCD camera. The content of each well was then plated to allow the number of CFU to be compared to the level of bioluminescence (RLU).

    Journal: Infection and Immunity

    Article Title: Monitoring Bioluminescent Staphylococcus aureus Infections in Living Mice Using a Novel luxABCDE Construct

    doi:

    Figure Lengend Snippet: Comparison of bioluminescence from S. aureus and E. coli containing the native luxCDABE operon versus that in strains containing the modified luxABCDE operon. Exponential cultures of S. aureus RN4220 pMK4 luxABCDE P1 (-■-), S. aureus RN4220 pMK4 luxCDABE P1 (-▴-), E. coli DH5α pMK4 luxABCDE P1 (‥■‥) and E. coli DH5α pMK4 luxCDABE P1 (‥▴‥) were diluted across black 96-well microtiter plates in doubling dilutions (−0.3 log) and monitored for light over a period of 30 min using the ICCD camera. The content of each well was then plated to allow the number of CFU to be compared to the level of bioluminescence (RLU).

    Article Snippet: This ligation was again electroporated into E. coli DH5α, and luxAB clones and selected by screening for bioluminescence in the presence of exogenously added n -decyl aldehyde (Sigma, St. Louis, Mo.).

    Techniques: Modification

    Temperature stability of the modified luxABCDE operon. Exponential cultures of S. aureus RN4220 pMK4 luxABCDE P1 (-■-), E. coli DH5α pMK4 luxABCDE P1 (‥■‥), and E. coli DH5α pMK4 luxCDABE P1 (‥▴‥) were grown to approximately 1 × 10 7 CFU/ml at 30°C and 1-ml volumes of each were placed in heating blocks set at 31, 33, 35, 37, 39, 41, 43, 45, and 47°C. After 1 h at each of these elevated temperatures, the nine heating blocks were sequentially placed inside a dark chamber and light from each of the three cultures was recorded for a period of 1 min using the ICCD camera. Shown are the number of RLU at each of the temperatures, with this data expressed as a percentage of the maximum (Max) bioluminescence attained and adjusted for variation in the number of CFU.

    Journal: Infection and Immunity

    Article Title: Monitoring Bioluminescent Staphylococcus aureus Infections in Living Mice Using a Novel luxABCDE Construct

    doi:

    Figure Lengend Snippet: Temperature stability of the modified luxABCDE operon. Exponential cultures of S. aureus RN4220 pMK4 luxABCDE P1 (-■-), E. coli DH5α pMK4 luxABCDE P1 (‥■‥), and E. coli DH5α pMK4 luxCDABE P1 (‥▴‥) were grown to approximately 1 × 10 7 CFU/ml at 30°C and 1-ml volumes of each were placed in heating blocks set at 31, 33, 35, 37, 39, 41, 43, 45, and 47°C. After 1 h at each of these elevated temperatures, the nine heating blocks were sequentially placed inside a dark chamber and light from each of the three cultures was recorded for a period of 1 min using the ICCD camera. Shown are the number of RLU at each of the temperatures, with this data expressed as a percentage of the maximum (Max) bioluminescence attained and adjusted for variation in the number of CFU.

    Article Snippet: This ligation was again electroporated into E. coli DH5α, and luxAB clones and selected by screening for bioluminescence in the presence of exogenously added n -decyl aldehyde (Sigma, St. Louis, Mo.).

    Techniques: Modification

    (A to C) Resistance of E. coli K12 strain DH5α (unshaded bars) with and without the espC -containing plasmid pJLM174 to lysozyme (A), lactoferrin (B), and human β-defensin 2 (hBD-2) (C). Transcription of espC from this pBAD construct is

    Journal: Applied and Environmental Microbiology

    Article Title: The Plasmid-Encoded Regulator Activates Factors Conferring Lysozyme Resistance on Enteropathogenic Escherichia coli Strains ▿

    doi: 10.1128/AEM.01734-08

    Figure Lengend Snippet: (A to C) Resistance of E. coli K12 strain DH5α (unshaded bars) with and without the espC -containing plasmid pJLM174 to lysozyme (A), lactoferrin (B), and human β-defensin 2 (hBD-2) (C). Transcription of espC from this pBAD construct is

    Article Snippet: We incubated bacterial supernatants of EPEC strain E2348/69 and of induced and uninduced E. coli DH5α(pJLM174), as well as of DH5α, with biotinylated lysozyme (Sigma).

    Techniques: Plasmid Preparation, Construct

    (A) Sequence of lysozyme with adjacent basic residues, hypothetical EspC target cleavage sites, underlined and in boldface. Lysozyme was incubated with DH5α carrying the espC expression clone pJLM174 activated with arabinose or repressed with

    Journal: Applied and Environmental Microbiology

    Article Title: The Plasmid-Encoded Regulator Activates Factors Conferring Lysozyme Resistance on Enteropathogenic Escherichia coli Strains ▿

    doi: 10.1128/AEM.01734-08

    Figure Lengend Snippet: (A) Sequence of lysozyme with adjacent basic residues, hypothetical EspC target cleavage sites, underlined and in boldface. Lysozyme was incubated with DH5α carrying the espC expression clone pJLM174 activated with arabinose or repressed with

    Article Snippet: We incubated bacterial supernatants of EPEC strain E2348/69 and of induced and uninduced E. coli DH5α(pJLM174), as well as of DH5α, with biotinylated lysozyme (Sigma).

    Techniques: Sequencing, Incubation, Expressing

    Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli DH5α; panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.

    Journal: Microbial Cell Factories

    Article Title: Bacillus thuringiensis subsp. kurstaki HD1 as a factory to synthesize alkali-labile ChiA74?sp chitinase inclusions, Cry crystals and spores for applied use

    doi: 10.1186/1475-2859-13-15

    Figure Lengend Snippet: Confirmation of the intracellular location of ChiA74∆s p in Escherichia coli and Bacillus thuringiensis 4Q7 using a chimeric construct with the green fluorescent protein (GFP) gene. (A) Schematic illustration that shows the fusion of chiA74 ∆s p with gfp . Panel 1, two constructs were developed, under regulation of the chiA74 wildtype promoter (wp) or under control of the cytA - p/ STAB-SD system. Lollipop indicates transcriptional terminator. Oligonucleotides used to make chimeric construct with gfp are shown in Table 1 . Panel 2, confirmation of chimeric constructs by PCR, primers used for amplification are showed in parenthesis and in Table 1 . L, 1 kb (kilobase) DNA Ladder (New England Biolabs); Lane 1, pEHchiA74∆ sp - gfp (primers: chiA74-1, chiA74-3); lane 2, pEHchiA74∆ sp - gfp (primers: chiA74-1, gfp-3); lane 3, pEHchiA74∆ sp - gfp (primers: gfp-1, chiA74-4); lane 4, pEB chi A74∆sp- gfp (primers: cytSTAB-1, chiA74-4); Lane 5, pEB chi A74∆sp- gfp (primers: cytSTAB-1, gfp-3); lane 6, pEB chi A74∆sp- gfp (primers: gfp-1, chiA74-4). (B) Phase contrast (left) and fluorescence micrographs (right) of recombinant strains of E. coli and B. thuringiensis strain 4Q7 expressing the chimeric protein ChiA74∆sp-GFP. Panel 1, E. coli- pEHchiA74∆ sp - gfp ; panel 2, 4Q7 - pEHchiA74∆ sp - gfp ; panel 3, E. coli- pEBchiA74∆ sp - gfp ; panel 4, 4Q7 - pEBchiA74∆ sp - gfp ; panel 5, E. coli DH5α; panel 6, 4Q7. Samples of recombinant strains of B. thuringiensis were collected at ~ 72 h.

    Article Snippet: All constructs (Figure A) were propagated in E. coli DH5α [supE44, DlacU169 (F80lacZDM15 ) hsdR17 recA1 end A1 gyrA96 thi-1 relA1 ] (Invitrogen, Carlsbad CA, USA) and then used for transforming the acrystalliferous strain of B. thuringiensis subsp. israelensis 4Q7, (hereafter 4Q7), and B. thuringiensis subsp. kurstaki HD1 (hereafter HD1) (Bacillus Genetic Stock Center, Columbus, OH).

    Techniques: Construct, Polymerase Chain Reaction, Amplification, Fluorescence, Recombinant, Expressing

    Expression of ChiA74Δsp in Escherichia coli DH5α and Bacillus thuringiensis 4Q7. (A) Schematic illustration of the strategy used to delete the secretion signal peptide-encoding sequence (shown as a rectangle inside the open reading frame) of chiA74 to generate chiA74∆ s p. Two constructs were developed, the first under regulation of wildtype promoter (wp) and the second under control of the strong cytA-p /STAB-SD promoter system. Lollipop indicates the putative transcriptional terminator site. Oligonucleotide sequences used to delete the signal peptide-coding sequence are shown in Table 1 . (B) Evaluation of endochitinase activity using solubilized intracellular proteins produced in E. coli. Panel 1: Zymogram using 4-MU-(GlcNAc) 3 for detection. Lane 1, E. coli- pEHchiA74∆s p ; lane 2, without sample; lane 3, E. coli- pEBchiA74∆s p; lane 4, E. coli DH5α . Black arrow indicates the position of ChiA74∆s p in recombinant E. coli strains . Panel 2: (a) E. coli- pEHchiA74∆s p , (b) E. coli- pEBchiA74∆s p. No endochitinase activity was observed with E. coli DH5α . (C) Phase contrast microscopy of recombinant strains of B. thuringiensis . Panel 1, 4Q7; panel 2, 4Q7 - pEHchiA74∆s p ; panel 3, 4Q7 - pEBchiA74∆s p . Black arrows indicate the positions of ChiA74∆s p inclusions. (D) Endochitinase activity determined using solubilized intracellular proteins of recombinant strains of B. thuringiensis. Panel 1: lane 1, 4Q7; lane 2, 4Q7 - pEHchiA74∆s p ; lane 3, 4Q7 - pEBchiA74∆s p. Black arrow indicates the position of ChiA74∆s p in recombinant 4Q7 strains . Panel 2: (a) 4Q7 - pEHchiA74∆s p , (b) 4Q7 - pEBchiA74∆s p . Activity of recombinant bacteria was normalized with the residual intracellular endochitinase activity of 4Q7.

    Journal: Microbial Cell Factories

    Article Title: Bacillus thuringiensis subsp. kurstaki HD1 as a factory to synthesize alkali-labile ChiA74?sp chitinase inclusions, Cry crystals and spores for applied use

    doi: 10.1186/1475-2859-13-15

    Figure Lengend Snippet: Expression of ChiA74Δsp in Escherichia coli DH5α and Bacillus thuringiensis 4Q7. (A) Schematic illustration of the strategy used to delete the secretion signal peptide-encoding sequence (shown as a rectangle inside the open reading frame) of chiA74 to generate chiA74∆ s p. Two constructs were developed, the first under regulation of wildtype promoter (wp) and the second under control of the strong cytA-p /STAB-SD promoter system. Lollipop indicates the putative transcriptional terminator site. Oligonucleotide sequences used to delete the signal peptide-coding sequence are shown in Table 1 . (B) Evaluation of endochitinase activity using solubilized intracellular proteins produced in E. coli. Panel 1: Zymogram using 4-MU-(GlcNAc) 3 for detection. Lane 1, E. coli- pEHchiA74∆s p ; lane 2, without sample; lane 3, E. coli- pEBchiA74∆s p; lane 4, E. coli DH5α . Black arrow indicates the position of ChiA74∆s p in recombinant E. coli strains . Panel 2: (a) E. coli- pEHchiA74∆s p , (b) E. coli- pEBchiA74∆s p. No endochitinase activity was observed with E. coli DH5α . (C) Phase contrast microscopy of recombinant strains of B. thuringiensis . Panel 1, 4Q7; panel 2, 4Q7 - pEHchiA74∆s p ; panel 3, 4Q7 - pEBchiA74∆s p . Black arrows indicate the positions of ChiA74∆s p inclusions. (D) Endochitinase activity determined using solubilized intracellular proteins of recombinant strains of B. thuringiensis. Panel 1: lane 1, 4Q7; lane 2, 4Q7 - pEHchiA74∆s p ; lane 3, 4Q7 - pEBchiA74∆s p. Black arrow indicates the position of ChiA74∆s p in recombinant 4Q7 strains . Panel 2: (a) 4Q7 - pEHchiA74∆s p , (b) 4Q7 - pEBchiA74∆s p . Activity of recombinant bacteria was normalized with the residual intracellular endochitinase activity of 4Q7.

    Article Snippet: All constructs (Figure A) were propagated in E. coli DH5α [supE44, DlacU169 (F80lacZDM15 ) hsdR17 recA1 end A1 gyrA96 thi-1 relA1 ] (Invitrogen, Carlsbad CA, USA) and then used for transforming the acrystalliferous strain of B. thuringiensis subsp. israelensis 4Q7, (hereafter 4Q7), and B. thuringiensis subsp. kurstaki HD1 (hereafter HD1) (Bacillus Genetic Stock Center, Columbus, OH).

    Techniques: Expressing, Sequencing, Construct, Activity Assay, Produced, Recombinant, Microscopy

    Strategy for gene replacement in Streptomyces . ( A )IV (Apra R ), oriT of RK2, and two FRT sites was amplified by PCR by using primers containing 39-nt 5′ homology extensions corresponding to regions flanking the S. coelicolor target gene (dotted lines a and b) and 20-nt 3′ homology to the unique priming sites P1 and P2 of the disruption cassette. ( B ) The PCR product from A was used to transform E. coli BW25113/pIJ790 (expressing the λ-Red recombination functions), containing the Supercos-1 cosmid with the cloned target gene ( cyc2 ). ( C ) Apra R transformants were selected, and the recombinant cosmid was identified by PCR and restriction analysis. ( D ) The potent methyl-specific restriction system of S. coelicolor was circumvented by introducing the recombinant cosmid into the methylation-deficient E. coli host ET12567/pUZ8002. The cosmid was mobilized in trans by pUZ8002 to Streptomyces by conjugation. ( E ) Selected exconjugants (Apra R ) were screened for Kan S (loss of the Kan resistance gene neo ), and the double cross-over allelic exchange was confirmed by PCR and Southern blot analysis. ( F ) E. coli DH5α cells containing pCP20 were transformed with the mutagenized cosmid DNA, and FLP synthesis was induced. ( G) The loss of the disruption resistance marker was tested, and the cosmid was introduced by polyethylene glycol (PEG)-mediated transformation into the S. coelicolor mutant carrying a marked deletion within the gene of interest. ( H ) Kan R transformants arising from single cross-over events were restreaked nonselectively and screened for the loss of both Kan R and Apra R , indicating a successful replacement by the in-frame deletion marked only by a “scar” sequence of 81 bp containing priming sites P1 and P2 (underlined), and one FRT site.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: PCR-targeted Streptomyces gene replacement identifies a protein domain needed for biosynthesis of the sesquiterpene soil odor geosmin

    doi: 10.1073/pnas.0337542100

    Figure Lengend Snippet: Strategy for gene replacement in Streptomyces . ( A )IV (Apra R ), oriT of RK2, and two FRT sites was amplified by PCR by using primers containing 39-nt 5′ homology extensions corresponding to regions flanking the S. coelicolor target gene (dotted lines a and b) and 20-nt 3′ homology to the unique priming sites P1 and P2 of the disruption cassette. ( B ) The PCR product from A was used to transform E. coli BW25113/pIJ790 (expressing the λ-Red recombination functions), containing the Supercos-1 cosmid with the cloned target gene ( cyc2 ). ( C ) Apra R transformants were selected, and the recombinant cosmid was identified by PCR and restriction analysis. ( D ) The potent methyl-specific restriction system of S. coelicolor was circumvented by introducing the recombinant cosmid into the methylation-deficient E. coli host ET12567/pUZ8002. The cosmid was mobilized in trans by pUZ8002 to Streptomyces by conjugation. ( E ) Selected exconjugants (Apra R ) were screened for Kan S (loss of the Kan resistance gene neo ), and the double cross-over allelic exchange was confirmed by PCR and Southern blot analysis. ( F ) E. coli DH5α cells containing pCP20 were transformed with the mutagenized cosmid DNA, and FLP synthesis was induced. ( G) The loss of the disruption resistance marker was tested, and the cosmid was introduced by polyethylene glycol (PEG)-mediated transformation into the S. coelicolor mutant carrying a marked deletion within the gene of interest. ( H ) Kan R transformants arising from single cross-over events were restreaked nonselectively and screened for the loss of both Kan R and Apra R , indicating a successful replacement by the in-frame deletion marked only by a “scar” sequence of 81 bp containing priming sites P1 and P2 (underlined), and one FRT site.

    Article Snippet: E. coli DH5α (Stratagene) was used as a host for plasmid constructions.

    Techniques: Amplification, Polymerase Chain Reaction, Expressing, Clone Assay, Recombinant, Methylation, Conjugation Assay, Southern Blot, Transformation Assay, Cosmid DNA, Marker, Mutagenesis, Sequencing

    Western blot analysis of urease extracts by using anti-UreB. Cytosolic protein extracts (10 μg) were electrophoresed through an SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with anti-UreB as described in the Materials and Methods section. (A) Extracts from DH5α (pHP8080/pBluescript) and DH5α (pHP8080/pUEFs). (B) Extracts from SE5000 (pHP8080/pBluescript) and SE5000 (pHP8080/pUDFs). Density values are shown below individual blots. Results are representative of three experiments performed by using extracts prepared on 3 separate days. pBS, pBluescript; 808, pHP808; 8080, pHP8080.

    Journal: Journal of Bacteriology

    Article Title: Isolation of Helicobacter pylori Genes That Modulate Urease Activity

    doi:

    Figure Lengend Snippet: Western blot analysis of urease extracts by using anti-UreB. Cytosolic protein extracts (10 μg) were electrophoresed through an SDS-polyacrylamide gel, transferred to a PVDF membrane, and probed with anti-UreB as described in the Materials and Methods section. (A) Extracts from DH5α (pHP8080/pBluescript) and DH5α (pHP8080/pUEFs). (B) Extracts from SE5000 (pHP8080/pBluescript) and SE5000 (pHP8080/pUDFs). Density values are shown below individual blots. Results are representative of three experiments performed by using extracts prepared on 3 separate days. pBS, pBluescript; 808, pHP808; 8080, pHP8080.

    Article Snippet: E. coli DH5α [F− supE44 Δ lacU169 (φ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 ] (Clontech, Palo Alto, Calif.), SE5000 [F− araD193 Δ( argF lac ) U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsR recA56 ] , XL1-Blue MRF′ ( thi-1 gyrA96 relA1 recA1 endA1 supE44 hsdR17 lac F′ [ proAB lacI q Z ΔM15 Tn 10 ]) (Stratagene, La Jolla, Calif.), and SURE ( thi-1 gyrA96 relA1 recJ recB endA1 sbcC lac Δ[ mcrCB-hsdMR-mrr ] 177 uvrC umu ::Tn 5 F′ [( proAB lacI q Z ΔM15 Tn 10 ]) (Clontech) were grown in Luria (L) broth and in Luria agar plus appropriate antibiotics (ampicillin [100 μg/ml], chloramphenicol [20 μg/ml], tetracycline [10 μg/ml], and/or kanamycin [50 μg/ml]) for maintenance of plasmids.

    Techniques: Western Blot

    a , b , c Response surface 3D model (left) and contour plot (right) to assessthe effects of the three variables on the yield of plasmid DNA vaccine pcDNA-CCOL2A1 produced by the engineered E. coli DH5α. a response surface plot showing the mutual effect of peptone and mannitol on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; b response surface plot showing the mutual effect of peptone and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; c response surface plot showing the mutual effect of mannitol and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1

    Journal: Journal of Biological Engineering

    Article Title: Optimization of fermentation conditions for an Escherichia coli strain engineered using the response surface method to produce a novel therapeutic DNA vaccine for rheumatoid arthritis

    doi: 10.1186/s13036-018-0110-y

    Figure Lengend Snippet: a , b , c Response surface 3D model (left) and contour plot (right) to assessthe effects of the three variables on the yield of plasmid DNA vaccine pcDNA-CCOL2A1 produced by the engineered E. coli DH5α. a response surface plot showing the mutual effect of peptone and mannitol on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; b response surface plot showing the mutual effect of peptone and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1; c response surface plot showing the mutual effect of mannitol and inoculum concentration on the yield of plasmid DNA vaccine pcDNA-CCOL2A1

    Article Snippet: The resulting recombinant plasmid containing an ampicillin resistance gene for selection was cloned in E. coli DH5α (CB101; Tiangen, Beijing, China).

    Techniques: Plasmid Preparation, Produced, Concentration Assay