e coli dh10b Thermo Fisher Search Results


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  • 99
    Thermo Fisher electromax e coli dh10b
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    Thermo Fisher e coli dh10b
    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli <t>DH10B.</t> A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p
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    Thermo Fisher competent escherichia coli
    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli <t>DH10B.</t> A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p
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    Thermo Fisher electrocompetent e coli
    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli <t>DH10B.</t> A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p
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    Thermo Fisher electrocompetent escherichia coli dh10b
    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli <t>DH10B.</t> A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p
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    Thermo Fisher competent escherichia coli dh10b
    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli <t>DH10B.</t> A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p
    Competent Escherichia Coli Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 88/100, based on 44 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher cultivation escherichia coli dh10b
    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli <t>DH10B.</t> A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p
    Cultivation Escherichia Coli Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 85/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher e coli strain dh10b
    Strategy for constructing a self-excisable RhCMV BAC. (A) Cloning of the full-length RhCMV genome into a BAC vector by Cre/lox recombination. The RhCMV genome structure with expansion of the US1-to-US3 region of recombinant viruses is diagrammed. The open box represents the internal junction between L and S components of the RhCMV genome. Cre/lox recombination was performed in Telo-RF cells. Recombinant clones containing the BAC vector in the viral genome (vRhCMV/BAC-EGFP) were plaque purified, and circular-form viral DNA was transformed into E. coli strain <t>DH10B</t> for plasmid isolation. (B) Substitution of the cre ORF for the egfp ORF by allelic exchange in E. coli . Only a portion of each plasmid is illustrated. The homologous flanking regions and the cre ORF of the recombination vector, pWC212, are illustrated. UL, unique sequences of the L component; US, unique sequences of the S component; PSV40, SV40 promoter; poly A, SV40 polyadenylation signal.
    E Coli Strain Dh10b, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 379 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Thermo Fisher e coli dh10b cells
    Stability of the BAC pBACBHV37 over multiple generations in E. coli <t>DH10B.</t> DH10B cells containing pBACBHV37 were serially grown for 5, 10, and 20 days. BAC DNA was prepared from these cultures, and the restriction enzyme profiles were compared by FIGE with 1% agarose. DNA fragments were stained with ethidium bromide and photographed. Standard molecular sizes (in base pairs) are given.
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    Thermo Fisher escherichia coli dh10b bacteria
    Stability of the BAC pBACBHV37 over multiple generations in E. coli <t>DH10B.</t> DH10B cells containing pBACBHV37 were serially grown for 5, 10, and 20 days. BAC DNA was prepared from these cultures, and the restriction enzyme profiles were compared by FIGE with 1% agarose. DNA fragments were stained with ethidium bromide and photographed. Standard molecular sizes (in base pairs) are given.
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    Stability of the BAC pBACBHV37 over multiple generations in E. coli <t>DH10B.</t> DH10B cells containing pBACBHV37 were serially grown for 5, 10, and 20 days. BAC DNA was prepared from these cultures, and the restriction enzyme profiles were compared by FIGE with 1% agarose. DNA fragments were stained with ethidium bromide and photographed. Standard molecular sizes (in base pairs) are given.
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    Thermo Fisher megax dh10b e coli
    Stability of the BAC pBACBHV37 over multiple generations in E. coli <t>DH10B.</t> DH10B cells containing pBACBHV37 were serially grown for 5, 10, and 20 days. BAC DNA was prepared from these cultures, and the restriction enzyme profiles were compared by FIGE with 1% agarose. DNA fragments were stained with ethidium bromide and photographed. Standard molecular sizes (in base pairs) are given.
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    Image Search Results


    mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p

    Journal: BMC Microbiology

    Article Title: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

    doi: 10.1186/s12866-018-1319-0

    Figure Lengend Snippet: mTn5 transposon mutagenesis using an IPTG-controlled conditional suicide plasmid. a Diagram of plasmid pSNC-mTn5. pSNC-mTn5 is a derivative of pMMB-repF that contains a Kan R -tagged mTn5, a lac promoter-controlled hyperactive transposase gene ( tnp H ), and a sacB counter selection marker (with its own promoter). OE and IE are outside and inside ends of the mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. A “+” symbol for IPTG indicates that the inducer was added to the liquid culture, and a “+” symbol for Suc, Cam, and Kan indicates that the chemicals were added to the plates. Black columns represent plasmid retention frequencies, and the blue column represents Tn5 retention frequency. Results were average of three independent experiments, and bars represent mean ± SD (*p

    Article Snippet: E. coli DH10B was purchased from Invitrogen.

    Techniques: Mutagenesis, Plasmid Preparation, Selection, Marker, Chick Chorioallantoic Membrane Assay

    Generation of a P. aeruginosa insertion library with pSNC-mTn5ME. a Diagram of pSNC-mTn5ME, a derivative of pSNC-mTn5 that has MEs instead of OE and IE at the termini of mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. Results were average of three independent experiments, and bars represent mean ± SD (* p

    Journal: BMC Microbiology

    Article Title: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

    doi: 10.1186/s12866-018-1319-0

    Figure Lengend Snippet: Generation of a P. aeruginosa insertion library with pSNC-mTn5ME. a Diagram of pSNC-mTn5ME, a derivative of pSNC-mTn5 that has MEs instead of OE and IE at the termini of mTn5. b Plasmid and transposon retention frequencies in E. coli DH10B. Results were average of three independent experiments, and bars represent mean ± SD (* p

    Article Snippet: E. coli DH10B was purchased from Invitrogen.

    Techniques: Plasmid Preparation

    IPTG-controlled conditional suicide plasmids. a Plasmid pMMB208 and its conditional-suicide derivatives. pMMB208 contains an RSF1010 oriV for replication and an oriT for conjugation. Genes repA , mobA / repB and repC encode proteins required for plasmid replication, and repF encodes a transcription repressor that binds promoter P4. pMMB208 also has Cam R and lacI Q genes and a P tac promoter. Plasmid pMMB- repF is a derivative of pMMB208 that has a second copy of the repF gene inserted downstream of P tac . Plasmids pMMB- repA K42A and pMMB- repA D139A have a dominant-negative repA mutant gene, either K42A or D139A, inserted downstream of P tac . b Amount of E. coli DH10B cells retaining the indicated plasmids after 24 h growth in the absence of antibiotics, either with or without IPTG induction. Results were average of three independent experiments, and bars represent mean ± SD (standard deviation). * p

    Journal: BMC Microbiology

    Article Title: Efficient transposon mutagenesis mediated by an IPTG-controlled conditional suicide plasmid

    doi: 10.1186/s12866-018-1319-0

    Figure Lengend Snippet: IPTG-controlled conditional suicide plasmids. a Plasmid pMMB208 and its conditional-suicide derivatives. pMMB208 contains an RSF1010 oriV for replication and an oriT for conjugation. Genes repA , mobA / repB and repC encode proteins required for plasmid replication, and repF encodes a transcription repressor that binds promoter P4. pMMB208 also has Cam R and lacI Q genes and a P tac promoter. Plasmid pMMB- repF is a derivative of pMMB208 that has a second copy of the repF gene inserted downstream of P tac . Plasmids pMMB- repA K42A and pMMB- repA D139A have a dominant-negative repA mutant gene, either K42A or D139A, inserted downstream of P tac . b Amount of E. coli DH10B cells retaining the indicated plasmids after 24 h growth in the absence of antibiotics, either with or without IPTG induction. Results were average of three independent experiments, and bars represent mean ± SD (standard deviation). * p

    Article Snippet: E. coli DH10B was purchased from Invitrogen.

    Techniques: Plasmid Preparation, Conjugation Assay, Chick Chorioallantoic Membrane Assay, Dominant Negative Mutation, Mutagenesis, Standard Deviation

    Strategy for constructing a self-excisable RhCMV BAC. (A) Cloning of the full-length RhCMV genome into a BAC vector by Cre/lox recombination. The RhCMV genome structure with expansion of the US1-to-US3 region of recombinant viruses is diagrammed. The open box represents the internal junction between L and S components of the RhCMV genome. Cre/lox recombination was performed in Telo-RF cells. Recombinant clones containing the BAC vector in the viral genome (vRhCMV/BAC-EGFP) were plaque purified, and circular-form viral DNA was transformed into E. coli strain DH10B for plasmid isolation. (B) Substitution of the cre ORF for the egfp ORF by allelic exchange in E. coli . Only a portion of each plasmid is illustrated. The homologous flanking regions and the cre ORF of the recombination vector, pWC212, are illustrated. UL, unique sequences of the L component; US, unique sequences of the S component; PSV40, SV40 promoter; poly A, SV40 polyadenylation signal.

    Journal: Journal of Virology

    Article Title: Cloning of the Full-Length Rhesus Cytomegalovirus Genome as an Infectious and Self-Excisable Bacterial Artificial Chromosome for Analysis of Viral Pathogenesis

    doi: 10.1128/JVI.77.9.5073-5083.2003

    Figure Lengend Snippet: Strategy for constructing a self-excisable RhCMV BAC. (A) Cloning of the full-length RhCMV genome into a BAC vector by Cre/lox recombination. The RhCMV genome structure with expansion of the US1-to-US3 region of recombinant viruses is diagrammed. The open box represents the internal junction between L and S components of the RhCMV genome. Cre/lox recombination was performed in Telo-RF cells. Recombinant clones containing the BAC vector in the viral genome (vRhCMV/BAC-EGFP) were plaque purified, and circular-form viral DNA was transformed into E. coli strain DH10B for plasmid isolation. (B) Substitution of the cre ORF for the egfp ORF by allelic exchange in E. coli . Only a portion of each plasmid is illustrated. The homologous flanking regions and the cre ORF of the recombination vector, pWC212, are illustrated. UL, unique sequences of the L component; US, unique sequences of the S component; PSV40, SV40 promoter; poly A, SV40 polyadenylation signal.

    Article Snippet: After incubation at 4°C for 24 h, cellular DNA and proteins were precipitated by centrifugation at 15,000 × g and 4°C for 30 min. DNA in the supernatant was extracted three times with phenol-chloroform, ethanol precipitated, and transformed into E. coli strain DH10B (Invitrogen) by electroporation according to published methods ( ).

    Techniques: BAC Assay, Clone Assay, Plasmid Preparation, Recombinant, Purification, Transformation Assay, Isolation

    Stability of the BAC pBACBHV37 over multiple generations in E. coli DH10B. DH10B cells containing pBACBHV37 were serially grown for 5, 10, and 20 days. BAC DNA was prepared from these cultures, and the restriction enzyme profiles were compared by FIGE with 1% agarose. DNA fragments were stained with ethidium bromide and photographed. Standard molecular sizes (in base pairs) are given.

    Journal: Journal of Virology

    Article Title: Construction and Manipulation of an Infectious Clone of the Bovine Herpesvirus 1 Genome Maintained as a Bacterial Artificial Chromosome

    doi: 10.1128/JVI.76.13.6660-6668.2002

    Figure Lengend Snippet: Stability of the BAC pBACBHV37 over multiple generations in E. coli DH10B. DH10B cells containing pBACBHV37 were serially grown for 5, 10, and 20 days. BAC DNA was prepared from these cultures, and the restriction enzyme profiles were compared by FIGE with 1% agarose. DNA fragments were stained with ethidium bromide and photographed. Standard molecular sizes (in base pairs) are given.

    Article Snippet: Aliquots of the ligation mixtures were electroporated into E. coli DH10B cells (1.5 kV, 100 Ω, 25 μF; Electroporator II; Invitrogen, San Diego, Calif.).

    Techniques: BAC Assay, Staining