e coli bl21 Stratagene Search Results


  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 76
    Stratagene escherichia coli e coli bl21 codonplus
    Escherichia Coli E Coli Bl21 Codonplus, supplied by Stratagene, used in various techniques. Bioz Stars score: 76/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli e coli bl21 codonplus/product/Stratagene
    Average 76 stars, based on 37 article reviews
    Price from $9.99 to $1999.99
    escherichia coli e coli bl21 codonplus - by Bioz Stars, 2020-01
    76/100 stars
      Buy from Supplier

    97
    Stratagene escherichia coli
    Escherichia Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 97/100, based on 1258 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli/product/Stratagene
    Average 97 stars, based on 1258 article reviews
    Price from $9.99 to $1999.99
    escherichia coli - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    95
    Stratagene escherichia coli bl21
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 95/100, based on 1417 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21/product/Stratagene
    Average 95 stars, based on 1417 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 - by Bioz Stars, 2020-01
    95/100 stars
      Buy from Supplier

    88
    Stratagene competent escherichia coli
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Competent Escherichia Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli/product/Stratagene
    Average 88 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    93
    Stratagene escherichia coli bl21 de3
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 278 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3/product/Stratagene
    Average 93 stars, based on 278 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    83
    Stratagene bl21 gold escherichia coli
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Bl21 Gold Escherichia Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 83/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 gold escherichia coli/product/Stratagene
    Average 83 stars, based on 68 article reviews
    Price from $9.99 to $1999.99
    bl21 gold escherichia coli - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    80
    Stratagene escherichia coli bl21 star
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 Star, supplied by Stratagene, used in various techniques. Bioz Stars score: 80/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 star/product/Stratagene
    Average 80 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 star - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    90
    Stratagene escherichia coli bl21 ril
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 Ril, supplied by Stratagene, used in various techniques. Bioz Stars score: 90/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 ril/product/Stratagene
    Average 90 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 ril - by Bioz Stars, 2020-01
    90/100 stars
      Buy from Supplier

    92
    Stratagene escherichia coli bl21 de3 ril
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3 Ril, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3 ril/product/Stratagene
    Average 92 stars, based on 111 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 ril - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    88
    Stratagene escherichia coli bl21 cells
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 88/100, based on 134 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 cells/product/Stratagene
    Average 88 stars, based on 134 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 cells - by Bioz Stars, 2020-01
    88/100 stars
      Buy from Supplier

    78
    Stratagene bl21 de3 codonplus escherichia coli
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Bl21 De3 Codonplus Escherichia Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 codonplus escherichia coli/product/Stratagene
    Average 78 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 codonplus escherichia coli - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    94
    Stratagene escherichia coli strain bl21
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Strain Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 94/100, based on 614 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain bl21/product/Stratagene
    Average 94 stars, based on 614 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain bl21 - by Bioz Stars, 2020-01
    94/100 stars
      Buy from Supplier

    80
    Stratagene bl21 codonplus ril escherichia coli
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Bl21 Codonplus Ril Escherichia Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 80/100, based on 38 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 codonplus ril escherichia coli/product/Stratagene
    Average 80 stars, based on 38 article reviews
    Price from $9.99 to $1999.99
    bl21 codonplus ril escherichia coli - by Bioz Stars, 2020-01
    80/100 stars
      Buy from Supplier

    85
    Stratagene escherichia coli bl21 de3 ripl
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3 Ripl, supplied by Stratagene, used in various techniques. Bioz Stars score: 85/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3 ripl/product/Stratagene
    Average 85 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 ripl - by Bioz Stars, 2020-01
    85/100 stars
      Buy from Supplier

    86
    Stratagene escherichia coli bl21 de3 plyss
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3 Plyss, supplied by Stratagene, used in various techniques. Bioz Stars score: 86/100, based on 78 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3 plyss/product/Stratagene
    Average 86 stars, based on 78 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 plyss - by Bioz Stars, 2020-01
    86/100 stars
      Buy from Supplier

    97
    Stratagene escherichia coli bl21 de3 gold
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3 Gold, supplied by Stratagene, used in various techniques. Bioz Stars score: 97/100, based on 108 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3 gold/product/Stratagene
    Average 97 stars, based on 108 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 gold - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    95
    Stratagene escherichia coli bl21 codon
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 Codon, supplied by Stratagene, used in various techniques. Bioz Stars score: 95/100, based on 115 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 codon/product/Stratagene
    Average 95 stars, based on 115 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 codon - by Bioz Stars, 2020-01
    95/100 stars
      Buy from Supplier

    76
    Stratagene e coli bl21 pril
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    E Coli Bl21 Pril, supplied by Stratagene, used in various techniques. Bioz Stars score: 76/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 pril/product/Stratagene
    Average 76 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 pril - by Bioz Stars, 2020-01
    76/100 stars
      Buy from Supplier

    77
    Stratagene competent escherichia coli bl21 de3
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Competent Escherichia Coli Bl21 De3, supplied by Stratagene, used in various techniques. Bioz Stars score: 77/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent escherichia coli bl21 de3/product/Stratagene
    Average 77 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    competent escherichia coli bl21 de3 - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    84
    Stratagene e coli bl21 λde3
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    E Coli Bl21 λde3, supplied by Stratagene, used in various techniques. Bioz Stars score: 84/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 λde3/product/Stratagene
    Average 84 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 λde3 - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    79
    Stratagene e coli bl21 plus
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    E Coli Bl21 Plus, supplied by Stratagene, used in various techniques. Bioz Stars score: 79/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/e coli bl21 plus/product/Stratagene
    Average 79 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    e coli bl21 plus - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    83
    Stratagene bl21 ripl e coli
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Bl21 Ripl E Coli, supplied by Stratagene, used in various techniques. Bioz Stars score: 83/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 ripl e coli/product/Stratagene
    Average 83 stars, based on 15 article reviews
    Price from $9.99 to $1999.99
    bl21 ripl e coli - by Bioz Stars, 2020-01
    83/100 stars
      Buy from Supplier

    97
    Stratagene competent e coli bl21
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Competent E Coli Bl21, supplied by Stratagene, used in various techniques. Bioz Stars score: 97/100, based on 84 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/competent e coli bl21/product/Stratagene
    Average 97 stars, based on 84 article reviews
    Price from $9.99 to $1999.99
    competent e coli bl21 - by Bioz Stars, 2020-01
    97/100 stars
      Buy from Supplier

    77
    Stratagene escherichia coli bl21 rilp cells
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 Rilp Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 77/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 rilp cells/product/Stratagene
    Average 77 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 rilp cells - by Bioz Stars, 2020-01
    77/100 stars
      Buy from Supplier

    84
    Stratagene bl21 de3 escherichia coli cells
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Bl21 De3 Escherichia Coli Cells, supplied by Stratagene, used in various techniques. Bioz Stars score: 84/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bl21 de3 escherichia coli cells/product/Stratagene
    Average 84 stars, based on 71 article reviews
    Price from $9.99 to $1999.99
    bl21 de3 escherichia coli cells - by Bioz Stars, 2020-01
    84/100 stars
      Buy from Supplier

    95
    Stratagene escherichia coli strain bl21 gold
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Strain Bl21 Gold, supplied by Stratagene, used in various techniques. Bioz Stars score: 95/100, based on 47 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain bl21 gold/product/Stratagene
    Average 95 stars, based on 47 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain bl21 gold - by Bioz Stars, 2020-01
    95/100 stars
      Buy from Supplier

    92
    Stratagene escherichia coli bl21 de3 codon
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3 Codon, supplied by Stratagene, used in various techniques. Bioz Stars score: 92/100, based on 88 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3 codon/product/Stratagene
    Average 92 stars, based on 88 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 codon - by Bioz Stars, 2020-01
    92/100 stars
      Buy from Supplier

    93
    Stratagene escherichia coli bl21 de3 codonplus ril
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3 Codonplus Ril, supplied by Stratagene, used in various techniques. Bioz Stars score: 93/100, based on 109 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3 codonplus ril/product/Stratagene
    Average 93 stars, based on 109 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 codonplus ril - by Bioz Stars, 2020-01
    93/100 stars
      Buy from Supplier

    99
    Stratagene escherichia coli strain bl21 codonplus
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Strain Bl21 Codonplus, supplied by Stratagene, used in various techniques. Bioz Stars score: 99/100, based on 168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain bl21 codonplus/product/Stratagene
    Average 99 stars, based on 168 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain bl21 codonplus - by Bioz Stars, 2020-01
    99/100 stars
      Buy from Supplier

    94
    Stratagene escherichia coli bl21 de3 strain
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 De3 Strain, supplied by Stratagene, used in various techniques. Bioz Stars score: 94/100, based on 66 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 de3 strain/product/Stratagene
    Average 94 stars, based on 66 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 de3 strain - by Bioz Stars, 2020-01
    94/100 stars
      Buy from Supplier

    78
    Stratagene escherichia coli bl21 codonplus bacteria
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Bl21 Codonplus Bacteria, supplied by Stratagene, used in various techniques. Bioz Stars score: 78/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli bl21 codonplus bacteria/product/Stratagene
    Average 78 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    escherichia coli bl21 codonplus bacteria - by Bioz Stars, 2020-01
    78/100 stars
      Buy from Supplier

    79
    Stratagene escherichia coli strain bl21 ril
    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli <t>BL21*CP</t> cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)
    Escherichia Coli Strain Bl21 Ril, supplied by Stratagene, used in various techniques. Bioz Stars score: 79/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/escherichia coli strain bl21 ril/product/Stratagene
    Average 79 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    escherichia coli strain bl21 ril - by Bioz Stars, 2020-01
    79/100 stars
      Buy from Supplier

    Image Search Results


    Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli BL21*CP cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Journal: FEBS Open Bio

    Article Title: Soluble cysteine-rich tick saliva proteins Salp15 and Iric-1 from E. coli

    doi: 10.1016/j.fob.2014.12.002

    Figure Lengend Snippet: Impact of fusion partner and E. coli expression strain on Salp15/Iric-1 solubility. (A) General structure of the expression constructs used. The Salp15 and Iric-1 sequences starting with A20 were fused to various solubility-enhancing domains (SEDs) carrying an N terminal His6-tag; in some constructs, a TEV protease recognition sequence was inserted in-between. ( B) Differential solubility enhancement by different protein tags. Total SDS-lysates versus the soluble fraction of native lysates from E. coli BL21*CP cultures expressing the indicated Salp15 fusion proteins were analyzed by SDS–PAGE followed by Coomassie Blue staining. (C) Impact of expression strain and Salp15 protein sequence. Total protein (T) in native lysates, and in the insoluble pellet (P) versus soluble supernatant (S) after centrifugation were analyzed by SDS–PAGE. Left panel: DsbA-Salp15 expressed in BL21*CP versus SHexpCP cells. Note the lower level of fusion protein in the insoluble pellet from SHexpCP cells. Right panel: DsbA-Salp15 versus DsbA-Iric-1 expressed in SHexpCP cells. Note the lower level of DsbA-Iric-1 in the insoluble pellet. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

    Article Snippet: 4.2 Recombinant tick protein and OspC expression in E. coli The respective Salp15 and Iric-1 expression vectors were transformed into E. coli BL21*CP (Stratagene) or T7 SHuffle express (NEB) cells carrying the CP rare tRNA plasmid.

    Techniques: Expressing, Solubility, Construct, Sequencing, SDS Page, Staining, Centrifugation